MD17G1074600.v1.1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006220
Physical Location & Seq
Forward (+)
6057665 .. 6060761
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1074600.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGCCTTACAAAGGATATGCTCAATCGAATCACTACGTAATCGGACAAAGGGACAGCAAATTTCGATGTGATTATATTCCGACTTCTGCAAATACTGCTAGAGAACATAAGAATCTAGGGATGGTTTGGTATGCTGCACCAGATTCCGGTACGAACGGGGAGCAACTGAGATCCTTGGATTCTTACTTTGGGAAGCTCCAAAATGTCACGAATCTGCCTCTGGATAATTCAAACAAGACAAGGGAGCAGCAACTCAGTAAAAATAGTGGTCAGCTGAGATTAAAGAAGGGTTTGGAGTCTCTTGATGATTATCTTGGAAAACTTAATAATAATGGTGGAGACTCGGCGAATTCGACATCTAGCTATTTTGATCTTACTACTGAAGAAATTCCAATTGCGAAACCATCAGCTATCATTCGAGATTCTGGAAATGGTGATAAGAGGGTACTGAAGACATATAGGAATCCTATAAGTAAAAGGGAAGATCTTGGTCCAGAGAATTCTCAAGATCCGCTCGAGTATAATGACATCTCTGATCTCTACTTAATAAGCATATTGGGTTCTGTAAACATTGCAGTGTTTCTGTTTGAGATTGCGAGTCCGGTTAGGAACACTGACTTAGGGCTCTTCTCTCTTCCATTGCTATATGGAGCAAAGATAAATGATTTGATCCTGGTTGGGGAATGGTGGAGGCTAGTCACACCCATGTTTCTGCACTCAGGACTTTTCCACATAGCTGTTGGTTGCTGGGGACTTGTTGCATTTGGGCCTAAAGTGTGCAGAGGCTATGGATCATTCACATTTTTCTTGATTTACATACTTGGAGGAGTCTCCGGCAACTTGATTAGCTTTCTTCATACACCAGAGCCAACTGTTGGCGGAACGGGACCAATATTCGCTATGATCGGAGCTTGGCTCGCTTATCACGTCCAGAACAAGGACATAATTGCAAAGGATGTTTCGGAGAGTATGTTCCAAAAGGCTGTCATAACCACGCTTCTTAGCTTCGTGTTGAGCATTTTCGGTCCTATTGATGATTGGACACATTTTGGAGCAGCATTTACAGGAGTGGCATATGGTTTTTTGACATGCCCAACTCTTCAACTGGATGATGCATCATCTTCTCCAGCGAGTGGGCAAGAGGAAGGAGTCGCACTTTTTAGACGATATGCAGATCCTTGCAAATCACTGTTTTTCTTTGCCCTATTTGTTCTTGTATTAAGCTCTATACTTTTCTTTGTTGAACCTCCTCTCGATGTCATAGCATCAGATACTTCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

428

Amino Acids

46.98

Weight (kDa)

5.43

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 227 - 365 7.2e-31 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 875, 1133
AccBSI CCGCTC 1 cut(s) 514
AciI CCGC 2 cut(s) 512, 879
AclWI GGATC 5 cut(s) 165, 503, 664, 799, 1169
AcsI RAATTY 4 cut(s) 59, 349, 387, 499
AcuI CTGAAG 2 cut(s) 402, 470
AfaI GTAC 2 cut(s) 151, 447
AfiI CCNNNNNNNGG 6 cut(s) 11, 146, 679, 875, 937, 1133
AgsI TTSAA 3 cut(s) 231, 1103, 1244
AjiI CACGTC 1 cut(s) 928
AjnI CCWGG 1 cut(s) 672
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
AluBI AGCT 9 cut(s) 196, 274, 363, 410, 737, 849, 911, 1005, 1224
AluI AGCT 9 cut(s) 196, 274, 363, 410, 737, 849, 911, 1005, 1224
Alw26I GTCTC 3 cut(s) 303, 333, 835
AlwI GGATC 5 cut(s) 165, 503, 664, 799, 1169
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 3 cut(s) 134, 247, 1055
ApoI RAATTY 4 cut(s) 59, 349, 387, 499
Asp700I GAANNNNTTC 1 cut(s) 387
AspS9I GGNCC 4 cut(s) 491, 767, 887, 1025
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 3 cut(s) 491, 887, 1025
BanII GRGCYC 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 458
BbvI GCAGC 3 cut(s) 121, 259, 1067
BccI CCATC 2 cut(s) 115, 412
BciT130I CCWGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 303, 333, 835
BfaI CTAG 4 cut(s) 99, 116, 360, 695
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 135, 248, 1056
BlsI GCNGC 3 cut(s) 136, 249, 1057
Bme1390I CCNGG 1 cut(s) 674
Bme18I GGWCC 3 cut(s) 491, 887, 1025
BmeT110I CYCGRG 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 928
BmgT120I GGNCC 4 cut(s) 491, 767, 887, 1025
BmiI GGNNCC 1 cut(s) 888
BmrFI CCNGG 1 cut(s) 674
BmsI GCATC 3 cut(s) 1102, 1124, 1274
BpiI GAAGAC 1 cut(s) 458
BplI GAGNNNNNCTC 2 cut(s) 900, 932
BpmI CTGGAG 1 cut(s) 1110
BpuEI CTTGAG 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 2 cut(s) 146, 601
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bsc4I CCNNNNNNNGG 6 cut(s) 11, 146, 679, 875, 937, 1133
Bse1I ACTGG 1 cut(s) 1110
Bse3DI GCAATG 2 cut(s) 570, 638
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 674
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 3 cut(s) 126, 961, 1114
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 6 cut(s) 11, 146, 679, 875, 937, 1133
BseMI GCAATG 2 cut(s) 570, 638
BseMII CTCAG 4 cut(s) 158, 266, 268, 732
BseNI ACTGG 1 cut(s) 1110
BseRI GAGGAG 2 cut(s) 840, 1239
BseXI GCAGC 3 cut(s) 121, 259, 1067
BseYI CCCAGC 1 cut(s) 747
BsgI GTGCAG 3 cut(s) 120, 698, 799
BshFI GGCC 1 cut(s) 769
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 147, 602, 834
BslFI GGGAC 3 cut(s) 65, 765, 900
BslI CCNNNNNNNGG 6 cut(s) 11, 146, 679, 875, 937, 1133
BsmAI GTCTC 3 cut(s) 303, 333, 835
BsmFI GGGAC 3 cut(s) 65, 765, 900
BsnI GGCC 1 cut(s) 769
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
BspACI CCGC 2 cut(s) 512, 879
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 4 cut(s) 159, 267, 267, 731
BspLI GGNNCC 1 cut(s) 888
BspPI GGATC 5 cut(s) 165, 503, 664, 799, 1169
BspQI GCTCTTC 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 514
BsrDI GCAATG 2 cut(s) 570, 638
BsrI ACTGG 1 cut(s) 1110
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 674
Bst4CI ACNGT 2 cut(s) 874, 1191
Bst6I CTCTTC 3 cut(s) 632, 639, 1104
BstAPI GCANNNNNTGC 1 cut(s) 95
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 918
BstDEI CTNAG 6 cut(s) 167, 254, 275, 619, 718, 1001
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 126, 961, 1114
BstKTI GATC 9 cut(s) 173, 373, 487, 511, 538, 672, 794, 906, 1177
BstMAI GTCTC 3 cut(s) 303, 333, 835
BstMBI GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 95, 917
BstNI CCWGG 1 cut(s) 674
BstNSI RCATGY 1 cut(s) 1092
BstSCI CCNGG 1 cut(s) 672
BstSNI TACGTA 1 cut(s) 37
BstV1I GCAGC 3 cut(s) 121, 259, 1067
BstV2I GAAGAC 1 cut(s) 458
BstX2I RGATCY 4 cut(s) 170, 484, 508, 1174
BstYI RGATCY 4 cut(s) 170, 484, 508, 1174
BsuRI GGCC 1 cut(s) 769
BtrI CACGTC 1 cut(s) 928
BtsCI GGATG 3 cut(s) 126, 961, 1114
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 582, 612, 1187
Cac8I GCNNGC 1 cut(s) 918
Cfr13I GGNCC 4 cut(s) 491, 767, 887, 1025
Csp6I GTAC 2 cut(s) 150, 446
CviAII CATG 2 cut(s) 706, 1089
CviQI GTAC 2 cut(s) 150, 446
DdeI CTNAG 6 cut(s) 167, 254, 275, 619, 718, 1001
DpnI GATC 9 cut(s) 172, 372, 486, 510, 537, 671, 793, 905, 1176
DpnII GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
Eam1104I CTCTTC 3 cut(s) 632, 639, 1104
EarI CTCTTC 3 cut(s) 632, 639, 1104
EciI GGCGGA 1 cut(s) 894
Eco105I TACGTA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 174
Eco24I GRGCYC 1 cut(s) 627
Eco47I GGWCC 3 cut(s) 491, 887, 1025
Eco57I CTGAAG 2 cut(s) 402, 470
Eco88I CYCGRG 1 cut(s) 515
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoRI GAATTC 2 cut(s) 349, 499
EcoRII CCWGG 1 cut(s) 672
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 1 cut(s) 1117
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 2 cut(s) 709, 1092
FaqI GGGAC 3 cut(s) 65, 765, 900
FatI CATG 2 cut(s) 705, 1088
FauNDI CATATG 1 cut(s) 1075
Fnu4HI GCNGC 3 cut(s) 135, 248, 1056
FokI GGATG 3 cut(s) 133, 968, 1121
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 3 cut(s) 135, 248, 1056
FspBI CTAG 4 cut(s) 99, 116, 360, 695
GluI GCNGC 3 cut(s) 135, 248, 1056
GsaI CCCAGC 1 cut(s) 751
GsuI CTGGAG 1 cut(s) 1110
HaeIII GGCC 1 cut(s) 769
HapII CCGG 3 cut(s) 147, 602, 834
Hin1II CATG 2 cut(s) 709, 1092
HpaII CCGG 3 cut(s) 147, 602, 834
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 6 cut(s) 44, 81, 535, 908, 964, 1270
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 280, 1139
HpyCH4III ACNGT 2 cut(s) 874, 1191
HpyCH4IV ACGT 2 cut(s) 36, 927
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 917
HpyF3I CTNAG 6 cut(s) 167, 254, 275, 619, 718, 1001
HpySE526I ACGT 2 cut(s) 36, 927
Hsp92II CATG 2 cut(s) 709, 1092
Kzo9I GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 6 cut(s) 160, 201, 244, 650, 908, 1052
Lsp1109I GCAGC 3 cut(s) 121, 259, 1067
LweI GCATC 3 cut(s) 1102, 1124, 1274
MaeI CTAG 4 cut(s) 99, 116, 360, 695
MaeII ACGT 2 cut(s) 36, 927
MaeIII GTNAC 2 cut(s) 205, 697
MalI GATC 9 cut(s) 172, 372, 486, 510, 537, 671, 793, 905, 1176
MbiI CCGCTC 1 cut(s) 514
MboI GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
MboII GAAGA 8 cut(s) 395, 463, 494, 619, 626, 845, 1091, 1113
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 4 cut(s) 170, 484, 508, 1174
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 7 cut(s) 59, 226, 349, 387, 393, 499, 945
MlyI GAGTC 5 cut(s) 305, 335, 607, 837, 1158
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 8 cut(s) 228, 435, 684, 776, 818, 1135, 1257, 1260
Mph1103I ATGCAT 1 cut(s) 1117
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 5 cut(s) 281, 324, 545, 1220, 1282
MslI CAYNNNNRTG 2 cut(s) 575, 704
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 3 cut(s) 147, 602, 834
MspR9I CCNGG 1 cut(s) 674
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 674
MwoI GCNNNNNNNGC 2 cut(s) 95, 917
NdeI CATATG 1 cut(s) 1075
NdeII GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
NlaIII CATG 2 cut(s) 709, 1092
NlaIV GGNNCC 1 cut(s) 888
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 2 cut(s) 205, 697
NsiI ATGCAT 1 cut(s) 1117
NspI RCATGY 1 cut(s) 1092
PaeR7I CTCGAG 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 350, 924
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 7 cut(s) 28, 112, 143, 179, 211, 422, 463
PflMI CCANNNNNTGG 2 cut(s) 875, 1133
PkrI GCNGC 3 cut(s) 136, 249, 1057
PleI GAGTC 5 cut(s) 304, 335, 606, 836, 1157
PpsI GAGTC 5 cut(s) 304, 335, 606, 836, 1157
Ppu21I YACGTR 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 672
PspFI CCCAGC 1 cut(s) 747
PspGI CCWGG 1 cut(s) 672
PspN4I GGNNCC 1 cut(s) 888
PspPI GGNCC 4 cut(s) 491, 767, 887, 1025
PspXI VCTCGAGB 1 cut(s) 515
PsuI RGATCY 4 cut(s) 170, 484, 508, 1174
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 2 cut(s) 151, 447
RsaNI GTAC 2 cut(s) 150, 446
RseI CAYNNNNRTG 2 cut(s) 575, 704
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 5 cut(s) 281, 324, 545, 1220, 1282
SatI GCNGC 3 cut(s) 135, 248, 1056
Sau3AI GATC 9 cut(s) 170, 370, 484, 508, 535, 669, 791, 903, 1174
Sau96I GGNCC 4 cut(s) 491, 767, 887, 1025
SchI GAGTC 5 cut(s) 305, 335, 607, 837, 1158
ScrFI CCNGG 1 cut(s) 674
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 3 cut(s) 1102, 1124, 1274
Sfr274I CTCGAG 1 cut(s) 515
SinI GGWCC 3 cut(s) 491, 887, 1025
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 2 cut(s) 575, 704
SmlI CTYRAG 2 cut(s) 504, 515
SmoI CTYRAG 2 cut(s) 504, 515
SnaBI TACGTA 1 cut(s) 37
Sse9I AATT 7 cut(s) 59, 226, 349, 387, 393, 499, 945
SsiI CCGC 2 cut(s) 512, 879
SspI AATATT 1 cut(s) 894
SspMI CTAG 4 cut(s) 99, 116, 360, 695
StyD4I CCNGG 1 cut(s) 672
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 874, 1191
TaiI ACGT 2 cut(s) 39, 930
TaqI TCGA 6 cut(s) 26, 64, 353, 418, 516, 1254
TaqII GACCGA 1 cut(s) 1013
TasI AATT 7 cut(s) 59, 226, 349, 387, 393, 499, 945
TfiI GAWTC 7 cut(s) 28, 112, 143, 179, 211, 422, 463
Tru1I TTAA 5 cut(s) 281, 324, 545, 1220, 1282
Tru9I TTAA 5 cut(s) 281, 324, 545, 1220, 1282
TscAI CASTG 3 cut(s) 582, 619, 1194
TseFI GTSAC 2 cut(s) 205, 697
TseI GCWGC 3 cut(s) 134, 247, 1055
Tsp45I GTSAC 2 cut(s) 205, 697
TspDTI ATGAA 1 cut(s) 845
TspRI CASTG 3 cut(s) 582, 619, 1194
Van91I CCANNNNNTGG 2 cut(s) 875, 1133
VpaK11BI GGWCC 3 cut(s) 491, 887, 1025
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 4 cut(s) 59, 349, 387, 499
XceI RCATGY 1 cut(s) 1092
XcmI CCANNNNNNNNNTGG 1 cut(s) 737
XhoI CTCGAG 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 4 cut(s) 99, 116, 360, 695
Zsp2I ATGCAT 1 cut(s) 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.