Rmu_sc0003984.1_g000017

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003984.1
OG0006220
Physical Location & Seq
Reverse (-)
54001 .. 56922
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003984.1_g000017.1.cds

Sequence Viewer

Length: 1221 bp
atgaatgagaagcagctgagttctttagattcctactttgaaaaactccaaaatagcacaactttgcctccagaaaattcaagcaagacaactgagtcactcggtagaaaaaatggtcatttgggattggaggaggagttggagtatcttgatgcttatcttgaaaaacttgataaagatgcaaaccagattgaaaaaccagttgatcttgcaactggagacaatatagttccaaaatcatcatctatcattcaacaatccaaaagaggtgctgaaaggaaactggtcaagagctatacaaatacaactggtgataatggtcaagaaagcgaaatctctgatctctacctagtaagcatattgggttctataaacattgcagtttttctgtttgagctagcaagtccagttaggaattctgacttggagcttttttctcttccattgttatatggagcaaagataaatgatttgatcctggtcggagaatggtggaggctagtcacaccgatgtttctgcatgctggacttattcatatagctattggttcttggggacttgttacatttggacctaaggtgtgcagaggctatggttcattcacatttttcttgatctatatacttggaggaatctctggcaatttgattagctttctccacacaccagaaccaactgttggtggaacaggaccaatatttgcgatgatcggagcttggctcatttatgagacccaaaacaaagatataattgcaaaggaagtctcagagactctgttccaaaaggcaataataactacagctcttaccttcacattgagcagttttggtccaattgatgactggacacattttggagctgcattgacaggaatagcatatgggtttttgacatgcccaagtcttcaactggacgaagcatcatcatcttcaacgagtggccaagaggaaggaatcacacttgttaggcgttatgctgatccctgcaaatcactttttatcttcacagtgtgccccaaccgtcgcgttccggtccgggagaggatggtgatcagggctagcggcgtggctttatggcagtggtgtggtccagggttagagattggttgtttgatctcgagcaggcgaggagcggcggggtatggcgtgccggcagtgggttctggagtcgccattgctttcgctatctgggtgtacatacatgtacatttgcaagcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

406

Amino Acids

44.34

Weight (kDa)

5.48

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 1133
AccII CGCG 1 cut(s) 1026
AciI CCGC 3 cut(s) 1062, 1133, 1136
AclWI GGATC 2 cut(s) 469, 974
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 2 cut(s) 76, 415
AdeI CACNNNGTG 1 cut(s) 1011
AfaI GTAC 2 cut(s) 1196, 1206
AfiI CCNNNNNNNGG 2 cut(s) 680, 1157
AflIII ACRYGT 1 cut(s) 1201
AgsI TTSAA 7 cut(s) 41, 81, 164, 194, 254, 908, 933
AjnI CCWGG 2 cut(s) 477, 1090
AjuI GAANNNNNNNTTGG 4 cut(s) 407, 439, 663, 695
AluBI AGCT 9 cut(s) 16, 294, 397, 430, 542, 654, 716, 803, 860
AluI AGCT 9 cut(s) 16, 294, 397, 430, 542, 654, 716, 803, 860
Alw26I GTCTC 4 cut(s) 213, 725, 764, 769
AlwI GGATC 2 cut(s) 469, 974
Ama87I CYCGRG 1 cut(s) 1117
AoxI GGCC 1 cut(s) 940
ApeKI GCWGC 2 cut(s) 13, 860
ApoI RAATTY 2 cut(s) 76, 415
AspS9I GGNCC 5 cut(s) 572, 692, 830, 1033, 1088
AsuC2I CCSGG 1 cut(s) 1037
AsuHPI GGTGA 2 cut(s) 323, 1060
AsuNHI GCTAGC 2 cut(s) 397, 1058
AvaI CYCGRG 1 cut(s) 1117
AvaII GGWCC 5 cut(s) 572, 692, 830, 1033, 1088
AxyI CCTNAGG 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 896
BbvI GCAGC 2 cut(s) 25, 847
BccI CCATC 1 cut(s) 1039
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 2 cut(s) 479, 1092
BclI TGATCA 1 cut(s) 1050
BcnI CCSGG 1 cut(s) 1037
BcoDI GTCTC 4 cut(s) 213, 725, 764, 769
BfaI CTAG 4 cut(s) 350, 398, 500, 1059
BfmI CTRYAG 1 cut(s) 798
BisI GCNGC 4 cut(s) 14, 861, 1063, 1134
BlsI GCNGC 4 cut(s) 15, 862, 1064, 1135
Bme1390I CCNGG 3 cut(s) 479, 1037, 1092
Bme18I GGWCC 5 cut(s) 572, 692, 830, 1033, 1088
BmeT110I CYCGRG 1 cut(s) 1117
BmgT120I GGNCC 5 cut(s) 572, 692, 830, 1033, 1088
BmrFI CCNGG 3 cut(s) 479, 1037, 1092
BmsI GCATC 3 cut(s) 142, 169, 929
BmtI GCTAGC 2 cut(s) 401, 1062
BpiI GAAGAC 1 cut(s) 896
BplI GAGNNNNNCTC 2 cut(s) 705, 737
BpmI CTGGAG 3 cut(s) 54, 237, 1185
BpuMI CCSGG 1 cut(s) 1037
BsaBI GATNNNNATC 2 cut(s) 156, 1049
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 1091
BsaWI WCCGGW 1 cut(s) 1030
BsaXI ACNNNNNCTCC 6 cut(s) 52, 82, 122, 128, 152, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 1157
Bse118I RCCGGY 1 cut(s) 1150
Bse1I ACTGG 7 cut(s) 200, 220, 288, 313, 407, 848, 915
Bse21I CCTNAGG 1 cut(s) 576
Bse3DI GCAATG 2 cut(s) 375, 1173
Bse8I GATNNNNATC 2 cut(s) 156, 1049
BseBI CCWGG 2 cut(s) 479, 1092
BseDI CCNNGG 1 cut(s) 1091
BseGI GGATG 1 cut(s) 1050
BseJI GATNNNNATC 2 cut(s) 156, 1049
BseLI CCNNNNNNNGG 2 cut(s) 680, 1157
BseMI GCAATG 2 cut(s) 375, 1173
BseMII CTCAG 3 cut(s) 8, 84, 780
BseNI ACTGG 7 cut(s) 200, 220, 288, 313, 407, 848, 915
BseRI GAGGAG 3 cut(s) 146, 149, 1143
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 2 cut(s) 25, 847
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 1026
BshFI GGCC 1 cut(s) 942
BsiHKCI CYCGRG 1 cut(s) 1117
BsiSI CCGG 3 cut(s) 1031, 1036, 1151
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 2 cut(s) 680, 1157
BsmAI GTCTC 4 cut(s) 213, 725, 764, 769
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 1 cut(s) 942
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 1117
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp1407I TGTACA 2 cut(s) 1194, 1204
Bsp143I GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
BspACI CCGC 3 cut(s) 1062, 1133, 1136
BspANI GGCC 1 cut(s) 942
BspCNI CTCAG 3 cut(s) 9, 85, 779
BspFNI CGCG 1 cut(s) 1026
BspOI GCTAGC 2 cut(s) 401, 1062
BspPI GGATC 2 cut(s) 469, 974
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 1 cut(s) 1133
BsrDI GCAATG 2 cut(s) 375, 1173
BsrFI RCCGGY 1 cut(s) 1150
BsrGI TGTACA 2 cut(s) 1194, 1204
BsrI ACTGG 7 cut(s) 200, 220, 288, 313, 407, 848, 915
BssAI RCCGGY 1 cut(s) 1150
BssECI CCNNGG 1 cut(s) 1091
BssMI GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
Bst2UI CCWGG 2 cut(s) 479, 1092
Bst4CI ACNGT 3 cut(s) 679, 1009, 1022
Bst6I CTCTTC 1 cut(s) 444
BstAUI TGTACA 2 cut(s) 1194, 1204
BstC8I GCNNGC 7 cut(s) 399, 522, 1060, 1124, 1148, 1152, 1215
BstDEI CTNAG 4 cut(s) 17, 93, 576, 766
BstF5I GGATG 1 cut(s) 1050
BstFNI CGCG 1 cut(s) 1026
BstKTI GATC 8 cut(s) 208, 343, 477, 618, 711, 982, 1053, 1116
BstMAI GTCTC 4 cut(s) 213, 725, 764, 769
BstMBI GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
BstNI CCWGG 2 cut(s) 479, 1092
BstNSI RCATGY 3 cut(s) 524, 897, 1205
BstSCI CCNGG 3 cut(s) 477, 1035, 1090
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 1026
BstV1I GCAGC 2 cut(s) 25, 847
BstV2I GAAGAC 1 cut(s) 896
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 1 cut(s) 942
BtgZI GCGATG 1 cut(s) 719
BtsCI GGATG 1 cut(s) 1050
BtsI GCAGTG 2 cut(s) 1085, 1161
BtsIMutI CAGTG 3 cut(s) 1014, 1085, 1161
Cac8I GCNNGC 7 cut(s) 399, 522, 1060, 1124, 1148, 1152, 1215
Cfr10I RCCGGY 1 cut(s) 1150
Cfr13I GGNCC 5 cut(s) 572, 692, 830, 1033, 1088
CpoI CGGWCCG 1 cut(s) 1033
Csp6I GTAC 2 cut(s) 1195, 1205
CspI CGGWCCG 1 cut(s) 1033
CviAII CATG 3 cut(s) 521, 894, 1202
CviQI GTAC 2 cut(s) 1195, 1205
DdeI CTNAG 4 cut(s) 17, 93, 576, 766
DpnI GATC 8 cut(s) 207, 342, 476, 617, 710, 981, 1052, 1115
DpnII GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
DraIII CACNNNGTG 1 cut(s) 1011
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 940
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 5 cut(s) 572, 692, 830, 1033, 1088
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 1117
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 477, 1090
FaeI CATG 3 cut(s) 524, 897, 1205
FaqI GGGAC 1 cut(s) 570
FatI CATG 3 cut(s) 520, 893, 1201
FauI CCCGC 1 cut(s) 1129
FauNDI CATATG 1 cut(s) 880
FbaI TGATCA 1 cut(s) 1050
Fnu4HI GCNGC 4 cut(s) 14, 861, 1063, 1134
FokI GGATG 1 cut(s) 1057
Fsp4HI GCNGC 4 cut(s) 14, 861, 1063, 1134
FspBI CTAG 4 cut(s) 350, 398, 500, 1059
GluI GCNGC 4 cut(s) 14, 861, 1063, 1134
GsuI CTGGAG 3 cut(s) 54, 237, 1185
HaeIII GGCC 1 cut(s) 942
HapII CCGG 3 cut(s) 1031, 1036, 1151
Hin1II CATG 3 cut(s) 524, 897, 1205
HinfI GANTC 6 cut(s) 29, 95, 633, 772, 954, 1167
HpaII CCGG 3 cut(s) 1031, 1036, 1151
HphI GGTGA 2 cut(s) 323, 1060
Hpy166II GTNNAC 1 cut(s) 1195
Hpy188I TCNGA 5 cut(s) 340, 421, 485, 713, 769
Hpy188III TCNNGA 8 cut(s) 71, 149, 161, 289, 323, 613, 1117, 1164
Hpy8I GTNNAC 1 cut(s) 1195
Hpy99I CGWCG 1 cut(s) 1026
HpyAV CCTTC 2 cut(s) 820, 944
HpyCH4III ACNGT 3 cut(s) 679, 1009, 1022
HpyCH4V TGCA 9 cut(s) 182, 212, 380, 520, 585, 755, 863, 987, 1213
HpyF3I CTNAG 4 cut(s) 17, 93, 576, 766
Hsp92II CATG 3 cut(s) 524, 897, 1205
KroI GCCGGC 1 cut(s) 1150
KroNI GCCGGC 1 cut(s) 1152
Ksp22I TGATCA 1 cut(s) 1050
Kzo9I GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
LmnI GCTCC 5 cut(s) 427, 455, 713, 857, 1130
Lsp1109I GCAGC 2 cut(s) 25, 847
LweI GCATC 3 cut(s) 142, 169, 929
MaeI CTAG 4 cut(s) 350, 398, 500, 1059
MaeIII GTNAC 3 cut(s) 96, 502, 562
MalI GATC 8 cut(s) 207, 342, 476, 617, 710, 981, 1052, 1115
MbiI CCGCTC 1 cut(s) 1133
MboI GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
MboII GAAGA 4 cut(s) 431, 896, 921, 994
MfeI CAATTG 1 cut(s) 834
MhlI GDGCHC 1 cut(s) 1016
MlsI TGGCCA 1 cut(s) 942
MluCI AATT 5 cut(s) 76, 415, 643, 750, 834
MluNI TGGCCA 1 cut(s) 942
MlyI GAGTC 3 cut(s) 104, 766, 1176
MmeI TCCRAC 2 cut(s) 120, 463
Mox20I TGGCCA 1 cut(s) 942
MroNI GCCGGC 1 cut(s) 1150
MscI TGGCCA 1 cut(s) 942
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 942
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 3 cut(s) 1031, 1036, 1151
MspR9I CCNGG 3 cut(s) 479, 1037, 1092
MunI CAATTG 1 cut(s) 834
MvaI CCWGG 2 cut(s) 479, 1092
MvnI CGCG 1 cut(s) 1026
NaeI GCCGGC 1 cut(s) 1152
NciI CCSGG 1 cut(s) 1037
NdeI CATATG 1 cut(s) 880
NdeII GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
NgoMIV GCCGGC 1 cut(s) 1150
NheI GCTAGC 2 cut(s) 397, 1058
NlaIII CATG 3 cut(s) 524, 897, 1205
NmuCI GTSAC 2 cut(s) 96, 502
NspI RCATGY 3 cut(s) 524, 897, 1205
PaeI GCATGC 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 1117
PciI ACATGT 1 cut(s) 1201
PdiI GCCGGC 1 cut(s) 1152
PfeI GAWTC 3 cut(s) 29, 633, 954
PflMI CCANNNNNTGG 1 cut(s) 680
PfoI TCCNGGA 1 cut(s) 1035
PkrI GCNGC 4 cut(s) 15, 862, 1064, 1135
PleI GAGTC 3 cut(s) 103, 766, 1175
PpsI GAGTC 3 cut(s) 103, 766, 1175
PscI ACATGT 1 cut(s) 1201
Psp6I CCWGG 2 cut(s) 477, 1090
PspGI CCWGG 2 cut(s) 477, 1090
PspPI GGNCC 5 cut(s) 572, 692, 830, 1033, 1088
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 2 cut(s) 1196, 1206
RsaNI GTAC 2 cut(s) 1195, 1205
RseI CAYNNNNRTG 1 cut(s) 509
Rsr2I CGGWCCG 1 cut(s) 1033
RsrII CGGWCCG 1 cut(s) 1033
SatI GCNGC 4 cut(s) 14, 861, 1063, 1134
Sau3AI GATC 8 cut(s) 205, 340, 474, 615, 708, 979, 1050, 1113
Sau96I GGNCC 5 cut(s) 572, 692, 830, 1033, 1088
SchI GAGTC 3 cut(s) 104, 766, 1176
ScrFI CCNGG 3 cut(s) 479, 1037, 1092
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 3 cut(s) 142, 169, 929
SfcI CTRYAG 1 cut(s) 798
Sfr274I CTCGAG 1 cut(s) 1117
SinI GGWCC 5 cut(s) 572, 692, 830, 1033, 1088
SlaI CTCGAG 1 cut(s) 1117
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 1117
SmoI CTYRAG 1 cut(s) 1117
SphI GCATGC 1 cut(s) 524
Sse9I AATT 5 cut(s) 76, 415, 643, 750, 834
SsiI CCGC 3 cut(s) 1062, 1133, 1136
SspI AATATT 1 cut(s) 699
SspMI CTAG 4 cut(s) 350, 398, 500, 1059
StyD4I CCNGG 3 cut(s) 477, 1035, 1090
TaaI ACNGT 3 cut(s) 679, 1009, 1022
TaqI TCGA 1 cut(s) 1118
TasI AATT 5 cut(s) 76, 415, 643, 750, 834
TatI WGTACW 2 cut(s) 1194, 1204
TauI GCSGC 2 cut(s) 1065, 1136
TfiI GAWTC 3 cut(s) 29, 633, 954
TscAI CASTG 3 cut(s) 1014, 1085, 1161
TseFI GTSAC 2 cut(s) 96, 502
TseI GCWGC 2 cut(s) 13, 860
Tsp45I GTSAC 2 cut(s) 96, 502
TspDTI ATGAA 3 cut(s) 17, 524, 588
TspRI CASTG 3 cut(s) 1014, 1085, 1161
Van91I CCANNNNNTGG 1 cut(s) 680
VpaK11BI GGWCC 5 cut(s) 572, 692, 830, 1033, 1088
XapI RAATTY 2 cut(s) 76, 415
XceI RCATGY 3 cut(s) 524, 897, 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 840
XhoI CTCGAG 1 cut(s) 1117
XspI CTAG 4 cut(s) 350, 398, 500, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.