Rh2DG589400

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006220
Physical Location & Seq
Reverse (-)
82240854 .. 82243040
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG589400.1

Sequence Viewer

Length: 1266 bp
ATGGCTTTGGTTCCAATATGCTACAGAATACCATACAAAGGGCATATTCAATCCATACGTAATGTAATAAGCGGACCAAGTTCATCTAAAGGACTAGAAAGCCGTCTAAGATGCAGTTTAGCACATAATTCACAAGAACACAATCGTGGGATGGTTCGGTATGCATCAGATGCTAGAATGAATGAGAAGCAGCTGAGTTCTTTAGATTCCTACTTTGAAAAACTCCAAAATAGCACAACTTTGCCTCCAGAAAATTCAAGCAAGACAACTGAGCCACTCGGTAGAAAAAATGGTCATTTGGGATTGGAGGAGGAGTTGGAGTATCTTGATGCTTATCTTGAAAAACTTGATAAAGGTGCAAACCAGATTGAAAAACCAGTTGATTTTGCAACTGGAGACAATATAGTTCCAAAATCATCATCTATCATTCAACAATCCAAAAGAGGTGCTGAAAGGAAACTGGTCAAGAGCTATACAAATACAACTGATGATAATGGTCAAGATAGCGAAATCTCTGATCTCTACCTAGTAAGCATATTGGGTTCTATAAACGTTGCAGTTTTTCTGTTTGAGCTAGCAAGTCCAGTTAGGAATTCTGACTTGGAGCTTTTTTCTCTTCCATTGTTATATGGAGCAAAGATAAATGATTTGATCCTGGTCGGAGAATGGTGGAGGCTAGTCACACCGATGTTTCTGCATGCTGGACTTATTCATATAGCTATTGGTTCTTGGGGACTTGTTACATTTGGACCTAAGGTGTGCAGAGGCTATGGTTCATTCACATTTTTCTTGATCTATATACTTGGAGGAATCTCTGGCAATTTGATTAGCTTTCTCCACACACCAGAACCAACTGTTGGTGGAACAGGACCAATATTTGCGATGATCGGAGCTTGGCTCATTTATGAGACCCAAAACAAAGATATAATTGCAAAGGAAGTCTCAGAGACTATGTTCCAAAAGGCAATAATAACTACAGCTCTTACCTTCACATTGAGCAGTTTTGGTCCAATTGATGACTGGACACATTTTGGAGCTGCATTGACAGGAATAGCATATGGGTTTTTGACGTGCCCAAGTCTTCAACTGGACGAAGCATCATCATCTTCAACAAGTGGCCAAGAGGAAGGAATCACACTTGTTAGGCGTTATGCTGATCCCTGCAAATCACTTTTTCTCTTCACAGTGTTTGTTCTTGTTCTGAGTTCTCTACTTCTCTTTGTTGAACCTCCACTCAACGCCTTAGCATCAGACTCTTCTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

421

Amino Acids

46.2

Weight (kDa)

5.46

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 221 - 359 1.2e-32 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 857
AciI CCGC 1 cut(s) 72
AclI AACGTT 1 cut(s) 552
AclWI GGATC 2 cut(s) 646, 1151
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 2 cut(s) 253, 592
AfiI CCNNNNNNNGG 2 cut(s) 38, 857
AgsI TTSAA 9 cut(s) 50, 218, 258, 341, 371, 431, 1085, 1110, 1226
AjiI CACGTC 1 cut(s) 1071
AjnI CCWGG 1 cut(s) 654
AjuI GAANNNNNNNTTGG 4 cut(s) 584, 616, 840, 872
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 9 cut(s) 193, 471, 574, 607, 719, 831, 893, 980, 1037
AluI AGCT 9 cut(s) 193, 471, 574, 607, 719, 831, 893, 980, 1037
Alw26I GTCTC 4 cut(s) 390, 902, 941, 946
AlwI GGATC 2 cut(s) 646, 1151
AoxI GGCC 1 cut(s) 1117
ApeKI GCWGC 2 cut(s) 190, 1037
ApoI RAATTY 2 cut(s) 253, 592
AspS9I GGNCC 4 cut(s) 74, 749, 869, 1007
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 4 cut(s) 74, 749, 869, 1007
AxyI CCTNAGG 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 1076
BalI TGGCCA 1 cut(s) 1119
BbsI GAAGAC 1 cut(s) 1073
BbvI GCAGC 2 cut(s) 202, 1024
BccI CCATC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 87
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BciT130I CCWGG 1 cut(s) 656
BcoDI GTCTC 4 cut(s) 390, 902, 941, 946
BfaI CTAG 5 cut(s) 95, 174, 527, 575, 677
BfmI CTRYAG 2 cut(s) 22, 975
BisI GCNGC 2 cut(s) 191, 1038
BlsI GCNGC 2 cut(s) 192, 1039
Bme1390I CCNGG 1 cut(s) 656
Bme18I GGWCC 4 cut(s) 74, 749, 869, 1007
BmgBI CACGTC 1 cut(s) 1071
BmgT120I GGNCC 4 cut(s) 74, 749, 869, 1007
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 656
BmsI GCATC 6 cut(s) 101, 160, 173, 319, 1106, 1256
BmtI GCTAGC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 1073
BplI GAGNNNNNCTC 2 cut(s) 882, 914
BpmI CTGGAG 2 cut(s) 231, 414
Bpu10I CCTNAGC 1 cut(s) 1243
BsaAI YACGTR 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 902
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 299, 305, 329, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 857
Bse1I ACTGG 6 cut(s) 377, 397, 465, 584, 1025, 1092
Bse21I CCTNAGG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 656
BseGI GGATG 1 cut(s) 156
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 38, 857
BseMII CTCAG 4 cut(s) 185, 261, 957, 1193
BseNI ACTGG 6 cut(s) 377, 397, 465, 584, 1025, 1092
BseRI GAGGAG 2 cut(s) 323, 326
BseSI GKGCMC 1 cut(s) 1076
BseXI GCAGC 2 cut(s) 202, 1024
BsgI GTGCAG 1 cut(s) 781
BshFI GGCC 1 cut(s) 1119
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 2 cut(s) 38, 857
BsmAI GTCTC 4 cut(s) 390, 902, 941, 946
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 1 cut(s) 1119
Bso31I GGTCTC 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 1076
Bsp143I GATC 5 cut(s) 517, 651, 792, 885, 1156
BspACI CCGC 1 cut(s) 72
BspANI GGCC 1 cut(s) 1119
BspCNI CTCAG 4 cut(s) 186, 262, 956, 1194
BspLI GGNNCC 1 cut(s) 12
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 2 cut(s) 646, 1151
BspTNI GGTCTC 1 cut(s) 902
BsrI ACTGG 6 cut(s) 377, 397, 465, 584, 1025, 1092
BssMI GATC 5 cut(s) 517, 651, 792, 885, 1156
Bst2UI CCWGG 1 cut(s) 656
Bst4CI ACNGT 2 cut(s) 856, 1186
Bst6I CTCTTC 3 cut(s) 621, 1184, 1261
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 576, 699
BstDEI CTNAG 7 cut(s) 107, 194, 270, 753, 943, 1202, 1243
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 5 cut(s) 520, 654, 795, 888, 1159
BstMAI GTCTC 4 cut(s) 390, 902, 941, 946
BstMBI GATC 5 cut(s) 517, 651, 792, 885, 1156
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 656
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 654
BstSFI CTRYAG 2 cut(s) 22, 975
BstSLI GKGCMC 1 cut(s) 1076
BstSNI TACGTA 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 202, 1024
BstV2I GAAGAC 1 cut(s) 1073
Bsu36I CCTNAGG 1 cut(s) 753
BsuRI GGCC 1 cut(s) 1119
BtgZI GCGATG 1 cut(s) 896
BtrI CACGTC 1 cut(s) 1071
BtsCI GGATG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 1191
Cac8I GCNNGC 2 cut(s) 576, 699
Cfr13I GGNCC 4 cut(s) 74, 749, 869, 1007
CviAII CATG 1 cut(s) 698
DdeI CTNAG 7 cut(s) 107, 194, 270, 753, 943, 1202, 1243
DpnI GATC 5 cut(s) 519, 653, 794, 887, 1158
DpnII GATC 5 cut(s) 517, 651, 792, 885, 1156
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 3 cut(s) 621, 1184, 1261
EarI CTCTTC 3 cut(s) 621, 1184, 1261
Eco105I TACGTA 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 4 cut(s) 74, 749, 869, 1007
Eco81I CCTNAGG 1 cut(s) 753
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 654
EcoT22I ATGCAT 1 cut(s) 166
FaeI CATG 1 cut(s) 701
FaqI GGGAC 1 cut(s) 747
FatI CATG 1 cut(s) 697
FauNDI CATATG 1 cut(s) 1057
Fnu4HI GCNGC 2 cut(s) 191, 1038
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 191, 1038
FspBI CTAG 5 cut(s) 95, 174, 527, 575, 677
GluI GCNGC 2 cut(s) 191, 1038
GsuI CTGGAG 2 cut(s) 231, 414
HaeIII GGCC 1 cut(s) 1119
Hin1II CATG 1 cut(s) 701
HinfI GANTC 4 cut(s) 206, 810, 1131, 1253
Hpy188I TCNGA 8 cut(s) 169, 517, 598, 662, 890, 946, 1203, 1252
Hpy188III TCNNGA 6 cut(s) 248, 326, 338, 466, 500, 790
HpyAV CCTTC 2 cut(s) 997, 1121
HpyCH4III ACNGT 2 cut(s) 856, 1186
HpyCH4IV ACGT 3 cut(s) 58, 552, 1070
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 7 cut(s) 107, 194, 270, 753, 943, 1202, 1243
HpySE526I ACGT 3 cut(s) 58, 552, 1070
Hsp92II CATG 1 cut(s) 701
Kzo9I GATC 5 cut(s) 517, 651, 792, 885, 1156
LmnI GCTCC 4 cut(s) 604, 632, 890, 1034
Lsp1109I GCAGC 2 cut(s) 202, 1024
LweI GCATC 6 cut(s) 101, 160, 173, 319, 1106, 1256
MaeI CTAG 5 cut(s) 95, 174, 527, 575, 677
MaeII ACGT 3 cut(s) 58, 552, 1070
MaeIII GTNAC 2 cut(s) 679, 739
MalI GATC 5 cut(s) 519, 653, 794, 887, 1158
MboI GATC 5 cut(s) 517, 651, 792, 885, 1156
MboII GAAGA 5 cut(s) 608, 1073, 1098, 1171, 1248
MfeI CAATTG 1 cut(s) 1011
MhlI GDGCHC 1 cut(s) 1076
MlsI TGGCCA 1 cut(s) 1119
MluCI AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
MluNI TGGCCA 1 cut(s) 1119
MlyI GAGTC 1 cut(s) 1247
MmeI TCCRAC 2 cut(s) 297, 640
MnlI CCTC 9 cut(s) 255, 301, 304, 437, 666, 758, 800, 1117, 1239
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 1 cut(s) 166
MscI TGGCCA 1 cut(s) 1119
MslI CAYNNNNRTG 2 cut(s) 144, 686
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 656
MunI CAATTG 1 cut(s) 1011
MvaI CCWGG 1 cut(s) 656
MwoI GCNNNNNNNGC 1 cut(s) 170
NdeI CATATG 1 cut(s) 1057
NdeII GATC 5 cut(s) 517, 651, 792, 885, 1156
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 1 cut(s) 701
NlaIV GGNNCC 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 679
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 1 cut(s) 701
OliI CACNNNNGTG 1 cut(s) 144
PaeI GCATGC 1 cut(s) 701
PfeI GAWTC 3 cut(s) 206, 810, 1131
PflMI CCANNNNNTGG 1 cut(s) 857
PkrI GCNGC 2 cut(s) 192, 1039
PleI GAGTC 1 cut(s) 1247
PpsI GAGTC 1 cut(s) 1247
Ppu21I YACGTR 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 654
PspGI CCWGG 1 cut(s) 654
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 4 cut(s) 74, 749, 869, 1007
PvuII CAGCTG 1 cut(s) 193
RseI CAYNNNNRTG 2 cut(s) 144, 686
SatI GCNGC 2 cut(s) 191, 1038
Sau3AI GATC 5 cut(s) 517, 651, 792, 885, 1156
Sau96I GGNCC 4 cut(s) 74, 749, 869, 1007
SchI GAGTC 1 cut(s) 1247
ScrFI CCNGG 1 cut(s) 656
SduI GDGCHC 1 cut(s) 1076
SfaNI GCATC 6 cut(s) 101, 160, 173, 319, 1106, 1256
SfcI CTRYAG 2 cut(s) 22, 975
SinI GGWCC 4 cut(s) 74, 749, 869, 1007
SmiMI CAYNNNNRTG 2 cut(s) 144, 686
SnaBI TACGTA 1 cut(s) 59
SphI GCATGC 1 cut(s) 701
Sse9I AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
SsiI CCGC 1 cut(s) 72
SspI AATATT 1 cut(s) 876
SspMI CTAG 5 cut(s) 95, 174, 527, 575, 677
StyD4I CCNGG 1 cut(s) 654
TaaI ACNGT 2 cut(s) 856, 1186
TaiI ACGT 3 cut(s) 61, 555, 1073
TasI AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
TfiI GAWTC 3 cut(s) 206, 810, 1131
TscAI CASTG 1 cut(s) 1191
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 2 cut(s) 190, 1037
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 4 cut(s) 72, 194, 701, 765
TspRI CASTG 1 cut(s) 1191
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 4 cut(s) 74, 749, 869, 1007
XapI RAATTY 2 cut(s) 253, 592
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 1017
XspI CTAG 5 cut(s) 95, 174, 527, 575, 677
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.