Rh2BG579100

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006220
Physical Location & Seq
Reverse (-)
79647061 .. 79649836
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG579100.1

Sequence Viewer

Length: 1026 bp
ATGGCTTTGGTTCCAATATGCTACAGGATACCATACAAAGGGCATATTCAACCCATACGTAATGTAATAAGCGGACCAAGTTCATCTAAAGGACTAGAAAGCCGTCTAAGATGCAGTTTAGCACATAATTCACAAGAACACAATCGTGGGATCGTTCGGTATGCATCAGATGCTAGAATGAATGAGAAGCAGCTGAGTTCTTTAGATTCCTACTTTGAAAAACTCCAAAATAGCACAACTTTGCCTCCAGAAAATTCAAGCAAGACAACTGAGTCACTCGGTAGAAAAAATGGTCATTTGGGATTGGAGGAGGAGTTGGAGTATCTTGACGCTTATCTTGAAAAACTTGATAAAGATGCAAACCAGATTGAAAAACCAGTTGATCTTGCAACTGGAGACAATATAGTTCCAAAATCATCATCTATCATTCAACAATCCAAAAAAGGTGCTGAGAGGAAACTGGTCAAGAGCTATACAAATACAACTGATGATAATGGTCAAGATAGCGAAATCTCTGATCTCTACCTAGTAAGCATATTGGGTTCTATAAACATTGCAGTTTTTCTGTTTGAGATAGCAAGTCCAGTTAGGAATTCTGACGTGGAGCTTTTTTCTCTTCCATTGTTATATGGAGCAAAGATAAATGATTTGATCCTGGTCGGAGAATGGTGGAGGCTAGTCACACCGATGTTTCTGCATGCTGGACTTATTCATATAGCTATTGGTTCTTGGGGACTTGTTACATTTGGACCTAAGGTGTGCAGAGGCTATGGTTCATTCACATTTTTCTTGATCTATATTCTTGGAGGAATCTCTGGCAATTTGATTAGCTTTCTCCACACACCAGAACCAACTGTTGGTGGAACAGGACCAATATTTGCGATGATCGGAGCTTGGCTCATTTATGAGACCCAAAACAAAGATATAATTGCAAAGGAAGTCTCAGAGACTATGTTCCAAAAGGCAATAATAACTACAGCTCTTACCTTCACATTGAGCAGTTTTGGTCCAATTGATGACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

341

Amino Acids

37.73

Weight (kDa)

5.7

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 221 - 332 4.9e-27 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 857
AciI CCGC 1 cut(s) 72
AclWI GGATC 2 cut(s) 158, 646
AcsI RAATTY 2 cut(s) 253, 592
AfiI CCNNNNNNNGG 2 cut(s) 38, 857
AgsI TTSAA 6 cut(s) 50, 218, 258, 341, 371, 431
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 1 cut(s) 654
AjuI GAANNNNNNNTTGG 2 cut(s) 840, 872
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 7 cut(s) 193, 471, 607, 719, 831, 893, 980
AluI AGCT 7 cut(s) 193, 471, 607, 719, 831, 893, 980
Alw26I GTCTC 4 cut(s) 390, 902, 941, 946
AlwI GGATC 2 cut(s) 158, 646
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 2 cut(s) 253, 592
AspS9I GGNCC 4 cut(s) 74, 749, 869, 1007
AvaII GGWCC 4 cut(s) 74, 749, 869, 1007
AxyI CCTNAGG 1 cut(s) 753
BbvI GCAGC 1 cut(s) 202
BceAI ACGGC 1 cut(s) 87
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BciT130I CCWGG 1 cut(s) 656
BciVI GTATCC 1 cut(s) 21
BcoDI GTCTC 4 cut(s) 390, 902, 941, 946
BfaI CTAG 4 cut(s) 95, 174, 527, 677
BfmI CTRYAG 2 cut(s) 22, 975
BfuI GTATCC 1 cut(s) 21
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 656
Bme18I GGWCC 4 cut(s) 74, 749, 869, 1007
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 4 cut(s) 74, 749, 869, 1007
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 656
BmsI GCATC 4 cut(s) 101, 160, 173, 346
BplI GAGNNNNNCTC 2 cut(s) 882, 914
BpmI CTGGAG 2 cut(s) 231, 414
BsaAI YACGTR 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 902
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 299, 305, 329, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 857
Bse1I ACTGG 5 cut(s) 377, 397, 465, 584, 1025
Bse21I CCTNAGG 1 cut(s) 753
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 1 cut(s) 656
BseLI CCNNNNNNNGG 2 cut(s) 38, 857
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 4 cut(s) 185, 261, 441, 957
BseNI ACTGG 5 cut(s) 377, 397, 465, 584, 1025
BseRI GAGGAG 2 cut(s) 323, 326
BseXI GCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 781
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 2 cut(s) 38, 857
BsmAI GTCTC 4 cut(s) 390, 902, 941, 946
BsmFI GGGAC 1 cut(s) 747
Bso31I GGTCTC 1 cut(s) 902
Bsp143I GATC 6 cut(s) 150, 382, 517, 651, 792, 885
BspACI CCGC 1 cut(s) 72
BspCNI CTCAG 4 cut(s) 186, 262, 442, 956
BspLI GGNNCC 1 cut(s) 12
BspPI GGATC 2 cut(s) 158, 646
BspTNI GGTCTC 1 cut(s) 902
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 5 cut(s) 377, 397, 465, 584, 1025
BssMI GATC 6 cut(s) 150, 382, 517, 651, 792, 885
Bst2UI CCWGG 1 cut(s) 656
Bst4CI ACNGT 1 cut(s) 856
Bst6I CTCTTC 1 cut(s) 621
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 6 cut(s) 107, 194, 270, 450, 753, 943
BstKTI GATC 6 cut(s) 153, 385, 520, 654, 795, 888
BstMAI GTCTC 4 cut(s) 390, 902, 941, 946
BstMBI GATC 6 cut(s) 150, 382, 517, 651, 792, 885
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 656
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 654
BstSFI CTRYAG 2 cut(s) 22, 975
BstSNI TACGTA 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 202
Bsu36I CCTNAGG 1 cut(s) 753
BsuI GTATCC 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 896
BtrI CACGTC 1 cut(s) 601
Cac8I GCNNGC 1 cut(s) 699
Cfr13I GGNCC 4 cut(s) 74, 749, 869, 1007
CseI GACGC 1 cut(s) 338
CviAII CATG 1 cut(s) 698
DdeI CTNAG 6 cut(s) 107, 194, 270, 450, 753, 943
DpnI GATC 6 cut(s) 152, 384, 519, 653, 794, 887
DpnII GATC 6 cut(s) 150, 382, 517, 651, 792, 885
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
Eco105I TACGTA 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 4 cut(s) 74, 749, 869, 1007
Eco81I CCTNAGG 1 cut(s) 753
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 654
EcoT22I ATGCAT 1 cut(s) 166
FaeI CATG 1 cut(s) 701
FaqI GGGAC 1 cut(s) 747
FatI CATG 1 cut(s) 697
Fnu4HI GCNGC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 191
FspBI CTAG 4 cut(s) 95, 174, 527, 677
GluI GCNGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 231, 414
HgaI GACGC 1 cut(s) 338
Hin1II CATG 1 cut(s) 701
HinfI GANTC 3 cut(s) 206, 272, 810
Hpy188I TCNGA 6 cut(s) 169, 517, 598, 662, 890, 946
Hpy188III TCNNGA 6 cut(s) 248, 326, 338, 466, 500, 790
HpyAV CCTTC 1 cut(s) 997
HpyCH4III ACNGT 1 cut(s) 856
HpyCH4IV ACGT 2 cut(s) 58, 600
HpyCH4V TGCA 8 cut(s) 114, 164, 359, 389, 557, 697, 762, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 6 cut(s) 107, 194, 270, 450, 753, 943
HpySE526I ACGT 2 cut(s) 58, 600
Hsp92II CATG 1 cut(s) 701
Kzo9I GATC 6 cut(s) 150, 382, 517, 651, 792, 885
LmnI GCTCC 3 cut(s) 604, 632, 890
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 4 cut(s) 101, 160, 173, 346
MaeI CTAG 4 cut(s) 95, 174, 527, 677
MaeII ACGT 2 cut(s) 58, 600
MaeIII GTNAC 3 cut(s) 273, 679, 739
MalI GATC 6 cut(s) 152, 384, 519, 653, 794, 887
MboI GATC 6 cut(s) 150, 382, 517, 651, 792, 885
MboII GAAGA 1 cut(s) 608
MfeI CAATTG 1 cut(s) 1011
MluCI AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 297, 640
MnlI CCTC 7 cut(s) 255, 301, 304, 447, 666, 758, 800
Mph1103I ATGCAT 1 cut(s) 166
MslI CAYNNNNRTG 2 cut(s) 144, 686
MspA1I CMGCKG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 656
MunI CAATTG 1 cut(s) 1011
MvaI CCWGG 1 cut(s) 656
MwoI GCNNNNNNNGC 1 cut(s) 170
NdeII GATC 6 cut(s) 150, 382, 517, 651, 792, 885
NlaIII CATG 1 cut(s) 701
NlaIV GGNNCC 1 cut(s) 12
NmuCI GTSAC 2 cut(s) 273, 679
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 1 cut(s) 701
OliI CACNNNNGTG 1 cut(s) 144
PaeI GCATGC 1 cut(s) 701
PfeI GAWTC 2 cut(s) 206, 810
PflMI CCANNNNNTGG 1 cut(s) 857
PkrI GCNGC 1 cut(s) 192
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 654
PspGI CCWGG 1 cut(s) 654
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 4 cut(s) 74, 749, 869, 1007
PvuII CAGCTG 1 cut(s) 193
RseI CAYNNNNRTG 2 cut(s) 144, 686
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 6 cut(s) 150, 382, 517, 651, 792, 885
Sau96I GGNCC 4 cut(s) 74, 749, 869, 1007
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 656
SfaNI GCATC 4 cut(s) 101, 160, 173, 346
SfcI CTRYAG 2 cut(s) 22, 975
SinI GGWCC 4 cut(s) 74, 749, 869, 1007
SmiMI CAYNNNNRTG 2 cut(s) 144, 686
SnaBI TACGTA 1 cut(s) 59
SphI GCATGC 1 cut(s) 701
Sse9I AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
SsiI CCGC 1 cut(s) 72
SspI AATATT 1 cut(s) 876
SspMI CTAG 4 cut(s) 95, 174, 527, 677
StyD4I CCNGG 1 cut(s) 654
TaaI ACNGT 1 cut(s) 856
TaiI ACGT 2 cut(s) 61, 603
TasI AATT 6 cut(s) 127, 253, 592, 820, 927, 1011
TfiI GAWTC 2 cut(s) 206, 810
TseFI GTSAC 2 cut(s) 273, 679
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 2 cut(s) 273, 679
TspDTI ATGAA 4 cut(s) 72, 194, 701, 765
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 4 cut(s) 74, 749, 869, 1007
XapI RAATTY 2 cut(s) 253, 592
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 1017
XspI CTAG 4 cut(s) 95, 174, 527, 677
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.