MD09G1085700.v1.1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006220
Physical Location & Seq
Forward (+)
6109025 .. 6112153
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1085700.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGTTTGGTATGCCGCATCAGATTCTGGGAACCATTTGAGATCCTTGGATTCTTACTTTGGGAAGCTCCAAAACGTCACAAATGTGCCTCTGGATGATTCAAACACGACAAGGGAGCAACCACTCAGTAAAAATAATGGTCAGCTGAGATTAAAGGAGGGTTTGGAGTCTCTTGATGATTATCTTGGAAAACTAAGAAATAAAGATGGAGACTCGACGAAAACATCTAGTTCTGTTGATCTTGCTACTGAAGACATTCCAATTGCAAAACCATCAGCTATCAATCGGGATTCCGGAAAAGGTGATGAGAGGGTACTGAAGGCATATAGAAATCCTATACGTAAAAGGGATGATCTTGGTCCAAAGAATTCTACAGACTCGCTCGAGTATAATGAAATCTCTGATCTCTACTTGATAAGCATATTAGGTTCTGTAAACATTGCAGTTTTTCTGTTCGAGATTGCGAGTCCTGTTAGGAACACTGACTTAGGGCTATTCTCTCTTCCATTGCTATATGGAGCAAAGATAAATGATTTGATCCTGGTTGGAGAATGGTGGAGGCTAGTCACACCCATGTTTCTGCACTCGGGGCTTTTCCACATAGCTGTTGGTTGCTGGGGACTTGTTGTATTTGGGCCTAAAGTGTGCAGAGGCTATGGATCATTCACATTTTTCTTGATTTACATACTCGGAGGAGTCTCCGGCAACTTGATTAGCTTTCTTCATACACCAGAGCCCACTGTTGGTGGAACGGGACCAATATTCGCCATGATCGGAGCTTGGCTCGCTTATCAGGTCCAAAACAAGGACATAATTTCCAAGGATATTTCGGAGAATATGTTCCAAAAGGCTGTCATAACCACGCTTCTTAGCTTCACTTTGAGCATTTTCTGTCCTATTGATGATTGGACACATTTTGGAGCAGCATTTGCAGGAGTGGCATATGGGTTTTTGACATGCCCAACTCTTCAACTGGATGATGCATCATCTTCTTCAGCAAGTGGCCAAGAGGAAGGAATCGCACTTGTTAGACGCTATGCAGATCCTTGCAAATCACTGTTTTTCTTTGCCCTATTTGTTCTTGTATTAAGCTCTCTACTTTTGTTTGTGGAACCTCCCCTGAATGTCATAGCATCAGATTCTTCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

384

Amino Acids

41.97

Weight (kDa)

5.11

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 183 - 321 8.1e-32 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 1 cut(s) 15
AclWI GGATC 4 cut(s) 36, 532, 667, 1037
AcoI YGGCCR 1 cut(s) 1003
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 3 cut(s) 270, 338, 978
AfaI GTAC 1 cut(s) 315
AfiI CCNNNNNNNGG 2 cut(s) 743, 805
AgsI TTSAA 2 cut(s) 102, 971
AjnI CCWGG 1 cut(s) 540
AleI CACNNNNGTG 1 cut(s) 83
AloI GAACNNNNNNTCC 2 cut(s) 824, 856
AluBI AGCT 8 cut(s) 67, 145, 278, 605, 717, 779, 873, 1092
AluI AGCT 8 cut(s) 67, 145, 278, 605, 717, 779, 873, 1092
Alw26I GTCTC 3 cut(s) 174, 204, 703
AlwI GGATC 4 cut(s) 36, 532, 667, 1037
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 2 cut(s) 383, 586
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 2 cut(s) 635, 1003
ApeKI GCWGC 1 cut(s) 923
ApoI RAATTY 1 cut(s) 367
Asp700I GAANNNNTTC 2 cut(s) 255, 839
AspS9I GGNCC 4 cut(s) 359, 635, 755, 796
AsuHPI GGTGA 1 cut(s) 314
AvaI CYCGRG 2 cut(s) 383, 586
AvaII GGWCC 3 cut(s) 359, 755, 796
BalI TGGCCA 1 cut(s) 1005
BanII GRGCYC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 935
BccI CCATC 2 cut(s) 200, 280
BciT130I CCWGG 1 cut(s) 542
BcoDI GTCTC 3 cut(s) 174, 204, 703
BfaI CTAG 2 cut(s) 228, 563
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 2 cut(s) 15, 924
BlsI GCNGC 2 cut(s) 16, 925
Bme1390I CCNGG 1 cut(s) 542
Bme18I GGWCC 3 cut(s) 359, 755, 796
BmeT110I CYCGRG 2 cut(s) 383, 586
BmgT120I GGNCC 4 cut(s) 359, 635, 755, 796
BmiI GGNNCC 3 cut(s) 32, 756, 1113
BmrFI CCNGG 1 cut(s) 542
BmsI GCATC 4 cut(s) 26, 970, 992, 1142
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 768, 800
BsaAI YACGTR 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 45, 819
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 824, 854
Bsc4I CCNNNNNNNGG 2 cut(s) 743, 805
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 2 cut(s) 438, 506
Bse8I GATNNNNATC 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 1 cut(s) 542
BseDI CCNNGG 2 cut(s) 45, 819
BseGI GGATG 3 cut(s) 100, 355, 982
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 743, 805
BseMI GCAATG 2 cut(s) 438, 506
BseMII CTCAG 2 cut(s) 137, 139
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 1 cut(s) 708
BseXI GCAGC 1 cut(s) 935
BseYI CCCAGC 1 cut(s) 615
BsgI GTGCAG 2 cut(s) 566, 667
BshFI GGCC 2 cut(s) 637, 1005
BsiHKCI CYCGRG 2 cut(s) 383, 586
BsiSI CCGG 2 cut(s) 294, 702
BslFI GGGAC 2 cut(s) 633, 768
BslI CCNNNNNNNGG 2 cut(s) 743, 805
BsmAI GTCTC 3 cut(s) 174, 204, 703
BsmFI GGGAC 2 cut(s) 633, 768
BsnI GGCC 2 cut(s) 637, 1005
BsoBI CYCGRG 2 cut(s) 383, 586
Bsp1286I GDGCHC 1 cut(s) 738
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
BspACI CCGC 1 cut(s) 15
BspANI GGCC 2 cut(s) 637, 1005
BspCNI CTCAG 2 cut(s) 138, 138
BspEI TCCGGA 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 32, 756, 1113
BspPI GGATC 4 cut(s) 36, 532, 667, 1037
BsrDI GCAATG 2 cut(s) 438, 506
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 2 cut(s) 45, 819
BssMI GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
BssT1I CCWWGG 2 cut(s) 45, 819
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 2 cut(s) 742, 1059
Bst6I CTCTTC 2 cut(s) 507, 972
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 5 cut(s) 125, 146, 194, 487, 869
BstF5I GGATG 3 cut(s) 100, 355, 982
BstKTI GATC 8 cut(s) 44, 241, 355, 406, 540, 662, 774, 1045
BstMAI GTCTC 3 cut(s) 174, 204, 703
BstMBI GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
BstMWI GCNNNNNNNGC 4 cut(s) 589, 785, 929, 938
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 1 cut(s) 960
BstSCI CCNGG 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 372
BstSNI TACGTA 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 935
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 2 cut(s) 41, 1042
BstYI RGATCY 2 cut(s) 41, 1042
BsuRI GGCC 2 cut(s) 637, 1005
BtsCI GGATG 3 cut(s) 100, 355, 982
BtsIMutI CAGTG 3 cut(s) 480, 738, 1055
Cac8I GCNNGC 1 cut(s) 786
CaiI CAGNNNCTG 1 cut(s) 26
Cfr13I GGNCC 4 cut(s) 359, 635, 755, 796
CseI GACGC 1 cut(s) 1041
Csp6I GTAC 1 cut(s) 314
CviAII CATG 3 cut(s) 574, 769, 957
CviQI GTAC 1 cut(s) 314
DdeI CTNAG 5 cut(s) 125, 146, 194, 487, 869
DpnI GATC 8 cut(s) 43, 240, 354, 405, 539, 661, 773, 1044
DpnII GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
EaeI YGGCCR 1 cut(s) 1003
Eam1104I CTCTTC 2 cut(s) 507, 972
EarI CTCTTC 2 cut(s) 507, 972
Eco105I TACGTA 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 45, 819
Eco24I GRGCYC 1 cut(s) 738
Eco47I GGWCC 3 cut(s) 359, 755, 796
Eco57I CTGAAG 3 cut(s) 270, 338, 978
Eco88I CYCGRG 2 cut(s) 383, 586
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 45, 819
EcoT22I ATGCAT 1 cut(s) 985
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 45, 819
FaeI CATG 3 cut(s) 577, 772, 960
FaqI GGGAC 2 cut(s) 633, 768
FatI CATG 3 cut(s) 573, 768, 956
FauNDI CATATG 1 cut(s) 943
Fnu4HI GCNGC 2 cut(s) 15, 924
FokI GGATG 3 cut(s) 107, 362, 989
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 2 cut(s) 15, 924
FspBI CTAG 2 cut(s) 228, 563
GluI GCNGC 2 cut(s) 15, 924
GsaI CCCAGC 1 cut(s) 619
HaeIII GGCC 2 cut(s) 637, 1005
HapII CCGG 2 cut(s) 294, 702
HgaI GACGC 1 cut(s) 1041
Hin1II CATG 3 cut(s) 577, 772, 960
HpaII CCGG 2 cut(s) 294, 702
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 6 cut(s) 22, 403, 692, 776, 832, 1138
Hpy188III TCNNGA 6 cut(s) 92, 173, 287, 294, 457, 676
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 2 cut(s) 313, 1007
HpyCH4III ACNGT 2 cut(s) 742, 1059
HpyCH4IV ACGT 2 cut(s) 75, 340
HpyCH4V TGCA 8 cut(s) 266, 443, 583, 648, 932, 983, 1040, 1050
HpyF10VI GCNNNNNNNGC 4 cut(s) 589, 785, 929, 938
HpyF3I CTNAG 5 cut(s) 125, 146, 194, 487, 869
HpySE526I ACGT 2 cut(s) 75, 340
Hsp92II CATG 3 cut(s) 577, 772, 960
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
LmnI GCTCC 5 cut(s) 72, 115, 518, 776, 920
Lsp1109I GCAGC 1 cut(s) 935
LweI GCATC 4 cut(s) 26, 970, 992, 1142
MaeI CTAG 2 cut(s) 228, 563
MaeII ACGT 2 cut(s) 75, 340
MaeIII GTNAC 2 cut(s) 76, 565
MalI GATC 8 cut(s) 43, 240, 354, 405, 539, 661, 773, 1044
MboI GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
MboII GAAGA 7 cut(s) 263, 494, 713, 959, 981, 984, 1134
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 2 cut(s) 41, 1042
MhlI GDGCHC 1 cut(s) 738
MlsI TGGCCA 1 cut(s) 1005
MluCI AATT 3 cut(s) 261, 367, 813
MluNI TGGCCA 1 cut(s) 1005
MlyI GAGTC 5 cut(s) 176, 206, 371, 475, 705
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 8 cut(s) 99, 151, 303, 552, 644, 686, 1003, 1125
Mox20I TGGCCA 1 cut(s) 1005
Mph1103I ATGCAT 1 cut(s) 985
MroI TCCGGA 1 cut(s) 293
MroXI GAANNNNTTC 2 cut(s) 255, 839
MscI TGGCCA 1 cut(s) 1005
MseI TTAA 2 cut(s) 152, 1088
MslI CAYNNNNRTG 2 cut(s) 83, 572
Msp20I TGGCCA 1 cut(s) 1005
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 2 cut(s) 294, 702
MspR9I CCNGG 1 cut(s) 542
MunI CAATTG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 542
MwoI GCNNNNNNNGC 4 cut(s) 589, 785, 929, 938
NdeI CATATG 1 cut(s) 943
NdeII GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
NlaIII CATG 3 cut(s) 577, 772, 960
NlaIV GGNNCC 3 cut(s) 32, 756, 1113
NmuCI GTSAC 2 cut(s) 76, 565
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 1 cut(s) 960
OliI CACNNNNGTG 1 cut(s) 83
PaeR7I CTCGAG 1 cut(s) 383
PdmI GAANNNNTTC 2 cut(s) 255, 839
PfeI GAWTC 6 cut(s) 23, 50, 98, 290, 1017, 1139
PkrI GCNGC 2 cut(s) 16, 925
PleI GAGTC 5 cut(s) 175, 206, 371, 474, 704
PpsI GAGTC 5 cut(s) 175, 206, 371, 474, 704
Ppu21I YACGTR 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 540
PspFI CCCAGC 1 cut(s) 615
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 3 cut(s) 32, 756, 1113
PspPI GGNCC 4 cut(s) 359, 635, 755, 796
PspXI VCTCGAGB 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 2 cut(s) 41, 1042
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
RseI CAYNNNNRTG 2 cut(s) 83, 572
SaqAI TTAA 2 cut(s) 152, 1088
SatI GCNGC 2 cut(s) 15, 924
Sau3AI GATC 8 cut(s) 41, 238, 352, 403, 537, 659, 771, 1042
Sau96I GGNCC 4 cut(s) 359, 635, 755, 796
SchI GAGTC 5 cut(s) 176, 206, 371, 475, 705
ScrFI CCNGG 1 cut(s) 542
SduI GDGCHC 1 cut(s) 738
SfaNI GCATC 4 cut(s) 26, 970, 992, 1142
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 383
SinI GGWCC 3 cut(s) 359, 755, 796
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 83, 572
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SnaBI TACGTA 1 cut(s) 341
Sse9I AATT 3 cut(s) 261, 367, 813
SsiI CCGC 1 cut(s) 15
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 228, 563
StyD4I CCNGG 1 cut(s) 540
StyI CCWWGG 2 cut(s) 45, 819
TaaI ACNGT 2 cut(s) 742, 1059
TaiI ACGT 2 cut(s) 78, 343
TaqI TCGA 3 cut(s) 215, 384, 456
TasI AATT 3 cut(s) 261, 367, 813
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 6 cut(s) 23, 50, 98, 290, 1017, 1139
Tru1I TTAA 2 cut(s) 152, 1088
Tru9I TTAA 2 cut(s) 152, 1088
TscAI CASTG 3 cut(s) 487, 745, 1062
TseFI GTSAC 2 cut(s) 76, 565
TseI GCWGC 1 cut(s) 923
Tsp45I GTSAC 2 cut(s) 76, 565
TspDTI ATGAA 2 cut(s) 408, 713
TspRI CASTG 3 cut(s) 487, 745, 1062
VpaK11BI GGWCC 3 cut(s) 359, 755, 796
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 960
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XhoI CTCGAG 1 cut(s) 383
XmnI GAANNNNTTC 2 cut(s) 255, 839
XspI CTAG 2 cut(s) 228, 563
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.