AT5G38895

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006042
Physical Location & Seq
Forward (+)
15571254 .. 15573526
2273 bp
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UTR
Exon/CDS
Intron
AT5G38895.1

Sequence Viewer

Length: 666 bp
ATGGGTGCTTTCTGTTGTTGCTTTCAAGTTGATCTCTTTGAGAGTTATGTGAATCCAAACACTTCCATAACTAGGAACTGTCCTTGCCTTAATTGCTTCCTCCAGAGTTTCATGGATTTGTATGCTTCGTTGTTTAGTAGAGGGGGAATGCATCCAATACCTTCAACTGTAGAGACAGCTACAGTGATGAATTCAACAACTGCACTTGATGACTCTCTGTCTAGTGTATATCATTCTCCTCCAACACCATTGCCTTACGATGCTGATCCTAGGTACTTTCGTTTTGTTAAGGGCTCTAGTCATTCAGGTGAAGAATCAGAACCGTTAAGAGGTGATACTGAAATGAGTTCTGAAGCTTTGGGTGATGGTGGAGCCAAGTGGAGTAAGTCTGATTCTGAAGATGGTTCGAAAGAAGTGTATACGAAAGGTTCATCAACCTTTACAAAGTCGAAAACAATGCCGGGGATAGAAGTTTATTATGCAGATTCGGATGATGAAGATATTTGTCCAACCTGCCTTGATGATTACACTTTGGAGAATCCGAAGATAATCACCAAGTGTTCTCACCATTTCCACCTTAGCTGTATTTATGAATGGATGGAGAGAAGTGAAACTTGCCCCGTCTGTGGAAAGGTGATGGCATTTGATGAAAATGAAACTTCTTAG

Protein Analysis

221

Amino Acids

24.52

Weight (kDa)

4.52

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 166 - 209 3.5e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 168 - 209 5.7e-12 Ring finger domain
zf-ANAPC11 PF12861 168 - 216 7.3e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4_2 PF13923 169 - 209 1.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 169 - 209 4.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 169 - 207 5e-06 RING-type zinc-finger
zf-RING_5 PF14634 169 - 211 5.6e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 419
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 2 cut(s) 372, 417
AdeI CACNNNGTG 1 cut(s) 558
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 72, 329, 626
AgsI TTSAA 3 cut(s) 26, 165, 195
AhdI GACNNNNNGTC 1 cut(s) 217
AluBI AGCT 3 cut(s) 179, 356, 582
AluI AGCT 3 cut(s) 179, 356, 582
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 190
AspA2I CCTAGG 1 cut(s) 269
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 6 cut(s) 320, 344, 374, 544, 557, 646
AsuII TTCGAA 1 cut(s) 407
AvrII CCTAGG 1 cut(s) 269
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BanII GRGCYC 1 cut(s) 296
BccI CCATC 4 cut(s) 359, 395, 592, 631
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 167
BfaI CTAG 4 cut(s) 72, 222, 270, 297
BfmI CTRYAG 2 cut(s) 168, 180
BfuAI ACCTGC 1 cut(s) 521
BlnI CCTAGG 1 cut(s) 269
Bme1390I CCNGG 1 cut(s) 462
BmeRI GACNNNNNGTC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 160, 250
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 269, 461
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 329, 626
Bse3DI GCAATG 1 cut(s) 248
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 269, 461
BseGI GGATG 3 cut(s) 151, 496, 603
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 72, 329, 626
BseMI GCAATG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 461
BslI CCNNNNNNNGG 3 cut(s) 72, 329, 626
BsmAI GTCTC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 2 cut(s) 31, 265
BspLI GGNNCC 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 1 cut(s) 260
BspT104I TTCGAA 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 269, 461
BssMI GATC 2 cut(s) 31, 265
BssNAI GTATAC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 420
Bst4CI ACNGT 4 cut(s) 80, 169, 184, 324
BstBI TTCGAA 1 cut(s) 407
BstDEI CTNAG 2 cut(s) 578, 663
BstF5I GGATG 3 cut(s) 151, 496, 603
BstKTI GATC 2 cut(s) 34, 268
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 31, 265
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 2 cut(s) 168, 180
BstZ17I GTATAC 1 cut(s) 420
BtsCI GGATG 3 cut(s) 151, 496, 603
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 521
Csp6I GTAC 1 cut(s) 274
CviAII CATG 1 cut(s) 112
CviJI RGCY 5 cut(s) 179, 294, 356, 374, 582
CviKI_1 RGCY 5 cut(s) 179, 294, 356, 374, 582
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 578, 663
DpnI GATC 2 cut(s) 33, 267
DpnII GATC 2 cut(s) 31, 265
DraIII CACNNNGTG 1 cut(s) 558
DriI GACNNNNNGTC 1 cut(s) 217
Eam1105I GACNNNNNGTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 296
Eco57I CTGAAG 2 cut(s) 372, 417
EcoRI GAATTC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 269
EcoT22I ATGCAT 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 1 cut(s) 115
FaiI YATR 8 cut(s) 48, 68, 113, 123, 229, 420, 480, 591
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FatI CATG 1 cut(s) 111
FblI GTMKAC 1 cut(s) 419
FokI GGATG 3 cut(s) 138, 503, 610
FriOI GRGCYC 1 cut(s) 296
FspBI CTAG 4 cut(s) 72, 222, 270, 297
GsuI CTGGAG 1 cut(s) 86
HapII CCGG 1 cut(s) 461
Hin1II CATG 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 6 cut(s) 52, 212, 314, 392, 485, 538
HpaII CCGG 1 cut(s) 461
HphI GGTGA 6 cut(s) 320, 344, 374, 544, 557, 646
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 6 cut(s) 319, 352, 391, 397, 490, 543
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 1 cut(s) 420
HpyAV CCTTC 1 cut(s) 171
HpyCH4III ACNGT 4 cut(s) 80, 169, 184, 324
HpyCH4V TGCA 3 cut(s) 151, 203, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpyF3I CTNAG 2 cut(s) 578, 663
Hsp92II CATG 1 cut(s) 115
Kzo9I GATC 2 cut(s) 31, 265
LmnI GCTCC 1 cut(s) 371
LpnPI CCDG 4 cut(s) 116, 291, 474, 526
LweI GCATC 2 cut(s) 160, 250
MaeI CTAG 4 cut(s) 72, 222, 270, 297
MalI GATC 2 cut(s) 33, 267
MboI GATC 2 cut(s) 31, 265
MboII GAAGA 4 cut(s) 323, 410, 509, 556
MhlI GDGCHC 1 cut(s) 296
MluCI AATT 2 cut(s) 91, 190
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 266, 533
MnlI CCTC 4 cut(s) 110, 134, 249, 323
Mph1103I ATGCAT 1 cut(s) 153
MseI TTAA 3 cut(s) 90, 288, 326
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 462
NdeII GATC 2 cut(s) 31, 265
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 153
NspV TTCGAA 1 cut(s) 407
PctI GAATGC 1 cut(s) 153
PfeI GAWTC 5 cut(s) 52, 314, 392, 485, 538
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 373
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 3 cut(s) 90, 288, 326
Sau3AI GATC 2 cut(s) 31, 265
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 296
SfaNI GCATC 2 cut(s) 160, 250
SfcI CTRYAG 2 cut(s) 168, 180
SfuI TTCGAA 1 cut(s) 407
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 2 cut(s) 91, 190
SspMI CTAG 4 cut(s) 72, 222, 270, 297
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 4 cut(s) 80, 169, 184, 324
TaqI TCGA 2 cut(s) 407, 449
TasI AATT 2 cut(s) 91, 190
TfiI GAWTC 5 cut(s) 52, 314, 392, 485, 538
Tru1I TTAA 3 cut(s) 90, 288, 326
Tru9I TTAA 3 cut(s) 90, 288, 326
TscAI CASTG 1 cut(s) 189
TspDTI ATGAA 6 cut(s) 100, 203, 420, 510, 606, 663
TspRI CASTG 1 cut(s) 189
XapI RAATTY 1 cut(s) 190
XmaJI CCTAGG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 419
XspI CTAG 4 cut(s) 72, 222, 270, 297
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.