MD09G1074400.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006042
Physical Location & Seq
Reverse (-)
5168970 .. 5172664
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1074400.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGGTTCTGTTTGTTGTTGTTTGAATGCTGAGGATTTTGAAGATTATGTGAATCCAAACAGTTCTGCATATAGGGAATGTGTCTGTCTTCGTTGTTTTCTTCAGAACTTTCTAAATGTGTATACATCACTATTCAGAAGAGGGGAAGTGCATTCAATCCCCTCATCCATTCAGGGGGCTGCATCAATGAATTCCTCGGGATCACTTGACAACTCCTTATCTGACATGTATCGTTCGCCTCCAAGGCCCTTGCCTTATGATGCTGACCCTAGATACATCCGGTTACAGCGGGATGGGTTGATTTCTAGGCGGGAGAAGGGCTCAAGCCACTCTCATGAGGAGGCTGAACCTCTAAGAAGTGATACTGATGCAGATTCTGAAAGTTTGAGTACAGGAGAAAAATGGAATGGCTCTGCTTGTGAAGATGGGTCAATAGAACATCGTTCCAAGTCCTCAATGAAGTTATCATCAGCCAAAACCACAACTGGAGTTGGGAACTTCTATACATCATCAGAGGATGAGGATGTTTGCCCAACATGTCTTGAAGAATACACGCCAGAAAACCCCAAAATAATGACAAAATGCTCTCATCATTTTCACCTTGGTTGTATATATGAATGGATGGAGAGAAGTGAAAGCTGCCCTGTTTGTGGCAAGGTGATGGCATTCGATGAAACGACTTGA

Protein Analysis

228

Amino Acids

25.34

Weight (kDa)

4.95

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 174 - 217 2.8e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 176 - 217 1.1e-11 Ring finger domain
zf-ANAPC11 PF12861 176 - 224 4.4e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4_2 PF13923 177 - 217 4.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 177 - 217 5.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 177 - 219 2.8e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 177 - 215 4.2e-06 RING-type zinc-finger
zf-RING_11 PF17123 177 - 204 9e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 122
AciI CCGC 2 cut(s) 289, 310
AclWI GGATC 1 cut(s) 208
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 174, 650
AflIII ACRYGT 2 cut(s) 225, 536
AgsI TTSAA 4 cut(s) 25, 41, 156, 545
AluBI AGCT 1 cut(s) 639
AluI AGCT 1 cut(s) 639
AlwI GGATC 1 cut(s) 208
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 179, 639
ApoI RAATTY 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 590, 670
AvaI CYCGRG 1 cut(s) 196
BanII GRGCYC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 80
BbvCI CCTCAGC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 166, 626
BccI CCATC 4 cut(s) 287, 419, 616, 655
BfaI CTAG 2 cut(s) 270, 306
BglI GCCNNNNNGGC 1 cut(s) 244
BisI GCNGC 2 cut(s) 180, 640
BlsI GCNGC 2 cut(s) 181, 641
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 246
BmsI GCATC 3 cut(s) 191, 250, 358
BpiI GAAGAC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 307
BsaJI CCNNGG 3 cut(s) 195, 242, 601
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 650
Bse1I ACTGG 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 195, 242, 601
BseGI GGATG 6 cut(s) 164, 276, 298, 523, 529, 627
BseLI CCNNNNNNNGG 2 cut(s) 174, 650
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 166, 626
BshFI GGCC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 280
BslI CCNNNNNNNGG 2 cut(s) 174, 650
BsmI GAATGC 3 cut(s) 31, 151, 665
BsnI GGCC 1 cut(s) 247
BsoBI CYCGRG 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 1 cut(s) 200
BspACI CCGC 2 cut(s) 289, 310
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 334
BspPI GGATC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 195, 242, 601
BssMI GATC 1 cut(s) 200
BssNAI GTATAC 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 242, 601
Bst1107I GTATAC 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 30, 353
BstF5I GGATG 6 cut(s) 164, 276, 298, 523, 529, 627
BstKTI GATC 1 cut(s) 203
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 1 cut(s) 244
BstNSI RCATGY 2 cut(s) 229, 540
BstV1I GCAGC 2 cut(s) 166, 626
BstV2I GAAGAC 1 cut(s) 80
BstZ17I GTATAC 1 cut(s) 123
BsuRI GGCC 1 cut(s) 247
BtsCI GGATG 6 cut(s) 164, 276, 298, 523, 529, 627
CaiI CAGNNNCTG 1 cut(s) 377
CciI TCATGA 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 390
CviAII CATG 3 cut(s) 226, 335, 537
CviJI RGCY 8 cut(s) 179, 247, 321, 327, 344, 411, 473, 639
CviKI_1 RGCY 8 cut(s) 179, 247, 321, 327, 344, 411, 473, 639
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 2 cut(s) 30, 353
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 242, 601
Eco24I GRGCYC 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 190
EcoT14I CCWWGG 2 cut(s) 242, 601
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 2 cut(s) 242, 601
FaeI CATG 3 cut(s) 229, 338, 540
FatI CATG 3 cut(s) 225, 334, 536
FauI CCCGC 2 cut(s) 282, 303
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 180, 640
FokI GGATG 6 cut(s) 151, 263, 305, 530, 536, 634
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 180, 640
FspBI CTAG 2 cut(s) 270, 306
GluI GCNGC 2 cut(s) 180, 640
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 247
HapII CCGG 1 cut(s) 280
Hin1II CATG 3 cut(s) 229, 338, 540
HinfI GANTC 2 cut(s) 52, 374
HpaII CCGG 1 cut(s) 280
HphI GGTGA 2 cut(s) 590, 670
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 5 cut(s) 105, 137, 223, 379, 514
Hpy188III TCNNGA 3 cut(s) 198, 335, 542
Hpy8I GTNNAC 1 cut(s) 123
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 68, 151, 182, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 244
HpyF3I CTNAG 2 cut(s) 30, 353
Hsp92II CATG 3 cut(s) 229, 338, 540
Kzo9I GATC 1 cut(s) 200
LpnPI CCDG 6 cut(s) 158, 293, 378, 471, 570, 657
Lsp1109I GCAGC 2 cut(s) 166, 626
LweI GCATC 3 cut(s) 191, 250, 358
MaeI CTAG 2 cut(s) 270, 306
MaeIII GTNAC 1 cut(s) 282
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 6 cut(s) 53, 80, 92, 150, 434, 557
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 1 cut(s) 190
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 1 cut(s) 280
Mva1269I GAATGC 3 cut(s) 31, 151, 665
MwoI GCNNNNNNNGC 1 cut(s) 244
NdeII GATC 1 cut(s) 200
NlaIII CATG 3 cut(s) 229, 338, 540
NspI RCATGY 2 cut(s) 229, 540
PagI TCATGA 1 cut(s) 334
PciI ACATGT 2 cut(s) 225, 536
PctI GAATGC 3 cut(s) 31, 151, 665
PfeI GAWTC 2 cut(s) 52, 374
PkrI GCNGC 2 cut(s) 181, 641
PscI ACATGT 2 cut(s) 225, 536
PspPI GGNCC 1 cut(s) 246
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 333
SatI GCNGC 2 cut(s) 180, 640
Sau3AI GATC 1 cut(s) 200
Sau96I GGNCC 1 cut(s) 246
SduI GDGCHC 1 cut(s) 323
SetI ASST 4 cut(s) 352, 603, 641, 660
SfaNI GCATC 3 cut(s) 191, 250, 358
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
Sse9I AATT 1 cut(s) 190
SsiI CCGC 2 cut(s) 289, 310
SspMI CTAG 2 cut(s) 270, 306
StyI CCWWGG 2 cut(s) 242, 601
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 669
TasI AATT 1 cut(s) 190
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 2 cut(s) 52, 374
TseI GCWGC 2 cut(s) 179, 639
TspDTI ATGAA 3 cut(s) 203, 473, 630
XapI RAATTY 1 cut(s) 190
XceI RCATGY 2 cut(s) 229, 540
XmiI GTMKAC 1 cut(s) 122
XspI CTAG 2 cut(s) 270, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.