RchiOBHm_Chr2g0164901

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006042
Physical Location & Seq
Forward (+)
79961979 .. 79965757
3779 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53289

Sequence Viewer

Length: 684 bp
ATGGGCGCTGTCTGTTGTTGTTTGAATGCTGATGATTTTGAAGATTATGTGAATCCAAACAGTTCTGCATTGAGGAACTGTCCGTGTCTTGGTTGCTTTTTTCAAAACTTTGTAAATGTGTATTCATCACTGTTCAGAAGAGGGGAACTACAGTCGCTCCCCTCATCCATTCAGGGGGCTGCATCGATGACTTCTGCAGCCTCACTTGATAGCTCTTTATCTGACATGTATCGCTCTCCTCCAAGGCCCTTGCCTTATGATGCTGACCCCAGATACATCCGTTTGCAGCGGGATGGATTGGTTTCTAGGCGTGAGAAAGGCACTAGTCATTCTCATGAGGAGACTGAACCTCTAAGAAGTGATACTGATGCAGATTCAGAATGTTTGAGTACAGGAGACAAATGGAATGGATCTACTTGTGAAGATGGATCAAAAGAACATCATTCCAGGTCCTCGATGAAGTTGTCATCTGCAAAAGCAACAACCGGATTTGGGATCATTTATACATCTTCAGAAGAAGAGGATGTATGCCCAACATGTCTTGAAGAATATACTCCAGAAAACCCCAAAATAATGACAAAATGCTCTCATCATTATCATCTCGCTTGTATATATGAGTGGATGGAGAGAAGTGAAAGTTGTCCTGTTTGTGGCAAGGTCATGGCATTCGATGAAACGACTTAA

Protein Analysis

227

Amino Acids

25.23

Weight (kDa)

4.92

Isoelectric Point (pI)

65.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 172 - 217 1.5e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 175 - 224 6.9e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 176 - 217 3.8e-11 Ring finger domain
zf-C3HC4_2 PF13923 177 - 217 1.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 177 - 217 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 177 - 219 3.6e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 177 - 215 8.1e-06 RING-type zinc-finger
zf-RING_11 PF17123 177 - 204 8.6e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 289
AclWI GGATC 3 cut(s) 418, 436, 503
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
AflIII ACRYGT 2 cut(s) 225, 536
AgsI TTSAA 4 cut(s) 25, 41, 104, 545
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 1 cut(s) 446
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 335, 390
AlwI GGATC 3 cut(s) 418, 436, 503
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 3 cut(s) 179, 197, 286
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 246, 450
AvaII GGWCC 1 cut(s) 450
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BbvI GCAGC 3 cut(s) 166, 209, 298
BccI CCATC 3 cut(s) 287, 419, 616
BciT130I CCWGG 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 335, 390
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 2 cut(s) 306, 324
BfmI CTRYAG 2 cut(s) 149, 195
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 3 cut(s) 180, 198, 287
BlsI GCNGC 3 cut(s) 181, 199, 288
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 246, 450
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 3 cut(s) 191, 250, 358
BpmI CTGGAG 1 cut(s) 540
Bsa29I ATCGAT 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BseBI CCWGG 1 cut(s) 448
BseCI ATCGAT 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 5 cut(s) 164, 276, 298, 529, 627
BseLI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BseRI GAGGAG 2 cut(s) 228, 353
BseXI GCAGC 3 cut(s) 166, 209, 298
BshFI GGCC 1 cut(s) 247
BshVI ATCGAT 1 cut(s) 185
BsiSI CCGG 1 cut(s) 486
BslI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BsmAI GTCTC 2 cut(s) 335, 390
BsmI GAATGC 2 cut(s) 31, 665
BsnI GGCC 1 cut(s) 247
Bsp143I GATC 3 cut(s) 410, 428, 495
BspACI CCGC 1 cut(s) 289
BspANI GGCC 1 cut(s) 247
BspDI ATCGAT 1 cut(s) 185
BspHI TCATGA 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 3 cut(s) 418, 436, 503
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 410, 428, 495
BssT1I CCWWGG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 4 cut(s) 62, 80, 132, 153
Bst6I CTCTTC 2 cut(s) 133, 513
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 5 cut(s) 164, 276, 298, 529, 627
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 3 cut(s) 413, 431, 498
BstMAI GTCTC 2 cut(s) 335, 390
BstMBI GATC 3 cut(s) 410, 428, 495
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 2 cut(s) 229, 540
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 2 cut(s) 149, 195
BstV1I GCAGC 3 cut(s) 166, 209, 298
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
Bsu15I ATCGAT 1 cut(s) 185
BsuRI GGCC 1 cut(s) 247
BsuTUI ATCGAT 1 cut(s) 185
BtsCI GGATG 5 cut(s) 164, 276, 298, 529, 627
BtsIMutI CAGTG 1 cut(s) 128
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 246, 450
ClaI ATCGAT 1 cut(s) 185
Csp6I GTAC 1 cut(s) 390
CviAII CATG 4 cut(s) 226, 335, 537, 661
CviJI RGCY 4 cut(s) 179, 200, 213, 247
CviKI_1 RGCY 4 cut(s) 179, 200, 213, 247
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 1 cut(s) 353
DpnI GATC 3 cut(s) 412, 430, 497
DpnII GATC 3 cut(s) 410, 428, 495
Eam1104I CTCTTC 2 cut(s) 133, 513
EarI CTCTTC 2 cut(s) 133, 513
Eco130I CCWWGG 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 246, 450
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 4 cut(s) 229, 338, 540, 664
FatI CATG 4 cut(s) 225, 334, 536, 660
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 180, 198, 287
FokI GGATG 5 cut(s) 151, 263, 305, 536, 634
Fsp4HI GCNGC 3 cut(s) 180, 198, 287
FspBI CTAG 2 cut(s) 306, 324
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 180, 198, 287
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 247
HapII CCGG 1 cut(s) 486
HhaI GCGC 1 cut(s) 8
Hin1II CATG 4 cut(s) 229, 338, 540, 664
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HinfI GANTC 2 cut(s) 52, 374
HpaII CCGG 1 cut(s) 486
Hpy188I TCNGA 4 cut(s) 137, 223, 379, 514
Hpy188III TCNNGA 3 cut(s) 335, 542, 557
HpyCH4III ACNGT 4 cut(s) 62, 80, 132, 153
HpyCH4V TGCA 6 cut(s) 68, 182, 197, 286, 371, 473
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 4 cut(s) 229, 338, 540, 664
HspAI GCGC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 410, 428, 495
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 8 cut(s) 158, 283, 378, 433, 460, 499, 570, 657
Lsp1109I GCAGC 3 cut(s) 166, 209, 298
LweI GCATC 3 cut(s) 191, 250, 358
MaeI CTAG 2 cut(s) 306, 324
MalI GATC 3 cut(s) 412, 430, 497
MboI GATC 3 cut(s) 410, 428, 495
MboII GAAGA 7 cut(s) 53, 150, 434, 501, 527, 530, 557
MflI RGATCY 1 cut(s) 410
MnlI CCTC 9 cut(s) 66, 134, 172, 211, 249, 331, 360, 463, 514
MseI TTAA 1 cut(s) 682
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 2 cut(s) 31, 665
MvaI CCWGG 1 cut(s) 448
NdeII GATC 3 cut(s) 410, 428, 495
NlaIII CATG 4 cut(s) 229, 338, 540, 664
NspI RCATGY 2 cut(s) 229, 540
PagI TCATGA 1 cut(s) 334
PciI ACATGT 2 cut(s) 225, 536
PctI GAATGC 2 cut(s) 31, 665
PfeI GAWTC 2 cut(s) 52, 374
PkrI GCNGC 3 cut(s) 181, 199, 288
PpuMI RGGWCCY 1 cut(s) 450
PscI ACATGT 2 cut(s) 225, 536
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 446
PspGI CCWGG 1 cut(s) 446
PspPI GGNCC 2 cut(s) 246, 450
PspPPI RGGWCCY 1 cut(s) 450
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 1 cut(s) 682
SatI GCNGC 3 cut(s) 180, 198, 287
Sau3AI GATC 3 cut(s) 410, 428, 495
Sau96I GGNCC 2 cut(s) 246, 450
ScrFI CCNGG 1 cut(s) 448
SetI ASST 4 cut(s) 215, 352, 452, 660
SfaNI GCATC 3 cut(s) 191, 250, 358
SfcI CTRYAG 2 cut(s) 149, 195
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 333
SpeI ACTAGT 1 cut(s) 323
SsiI CCGC 1 cut(s) 289
SspMI CTAG 2 cut(s) 306, 324
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 4 cut(s) 62, 80, 132, 153
TaqI TCGA 3 cut(s) 185, 455, 669
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 2 cut(s) 52, 374
Tru1I TTAA 1 cut(s) 682
Tru9I TTAA 1 cut(s) 682
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 179, 197, 286
TspDTI ATGAA 2 cut(s) 114, 473
TspGWI ACGGA 2 cut(s) 72, 269
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 450
XceI RCATGY 2 cut(s) 229, 540
XspI CTAG 2 cut(s) 306, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.