FvH4_6g46360

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006042
Physical Location & Seq
Forward (+)
35393599 .. 35397135
3537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46360.t2

Sequence Viewer

Length: 684 bp
ATGGGTGCTGTCTGTTGTTGTTTGAATGCCGATGATTTCGAAGATTATGTGAATCCAAACAGTTCTGCATTGAGGAACTGTCCGTGTGCCAACTGCTGTGTTCAGAACTTTGTAAATGTGTATTCATCACTGTTCAGAAGAGGGGAACTACAGTCGCTCCCTTCATCCGTTCAGGGGGCTGCTTCAATAACTTCTGCAGCCTCACTTGATAGCACTTTATCTGACATGTATCGCTCTCCTCCAAGGCCCTTGCCTTATGATGTTGACCCCAGATACATCCGCTTGCAGCGGGATGGATTAGTTTCTAGGCGTGAGAAAGGCACCAGTCATTCTCGTGAGGAGACTGAACCTCTAAGAAGTGATACCGATGCAGATTCAGAATGTTTGAGTGCAGGAGACAAATGGAATGAAACTGCTTGTGAAGATGGATCCAAGGAACATCGTTGCAGGTCCTCAATGAAGTTGTCATCGGCAAAAACAACAACTGGATTTGGTATTGTTTATACATCTTCAGAAGAAGAGGATGTATGCCCAACATGTCTTGAAGAATATACTCCAGAAAACCCCAAAATATTGACAAAATGCTCTCATCATTATCATCTTGGTTGTATATATGAGTGGATGGAGAGAAGTGAAAGTTGTCCTGTATGTGGCAAGGTCATGGCATTCGATGAAACGACTTAA

Protein Analysis

228

Amino Acids

25.22

Weight (kDa)

4.88

Isoelectric Point (pI)

64.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 172 - 217 2.7e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 175 - 224 5.5e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 176 - 217 2.6e-11 Ring finger domain
zf-C3HC4_2 PF13923 177 - 217 4.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 177 - 215 1.4e-06 RING-type zinc-finger
zf-C3HC4 PF00097 177 - 217 1.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 177 - 219 3.7e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 320
AciI CCGC 2 cut(s) 280, 289
AclWI GGATC 2 cut(s) 423, 436
AcuI CTGAAG 1 cut(s) 495
AfiI CCNNNNNNNGG 2 cut(s) 174, 650
AflIII ACRYGT 2 cut(s) 225, 536
AgsI TTSAA 3 cut(s) 25, 186, 545
Alw26I GTCTC 2 cut(s) 335, 390
AlwI GGATC 2 cut(s) 423, 436
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 3 cut(s) 179, 197, 286
AspS9I GGNCC 2 cut(s) 246, 450
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 1 cut(s) 450
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 320
BauI CACGAG 1 cut(s) 333
BbvI GCAGC 3 cut(s) 166, 209, 298
BccI CCATC 3 cut(s) 287, 419, 616
BcoDI GTCTC 2 cut(s) 335, 390
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 2 cut(s) 149, 195
BfuAI ACCTGC 1 cut(s) 438
BisI GCNGC 3 cut(s) 180, 198, 287
BlsI GCNGC 3 cut(s) 181, 199, 288
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 246, 450
BmiI GGNNCC 2 cut(s) 322, 430
BmsI GCATC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 242, 432
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 650
Bse1I ACTGG 2 cut(s) 324, 490
BseDI CCNNGG 2 cut(s) 242, 432
BseGI GGATG 5 cut(s) 164, 276, 298, 529, 627
BseLI CCNNNNNNNGG 2 cut(s) 174, 650
BseNI ACTGG 2 cut(s) 324, 490
BseRI GAGGAG 2 cut(s) 228, 353
BseXI GCAGC 3 cut(s) 166, 209, 298
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 247
BshNI GGYRCC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 174, 650
BsmAI GTCTC 2 cut(s) 335, 390
BsmI GAATGC 2 cut(s) 31, 665
BsnI GGCC 1 cut(s) 247
Bsp119I TTCGAA 1 cut(s) 39
Bsp143I GATC 1 cut(s) 428
BspACI CCGC 2 cut(s) 280, 289
BspANI GGCC 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 322, 430
BspMAI CTGCAG 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 438
BspPI GGATC 2 cut(s) 423, 436
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 324, 490
BssECI CCNNGG 2 cut(s) 242, 432
BssMI GATC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 242, 432
Bst2BI CACGAG 1 cut(s) 333
Bst4CI ACNGT 4 cut(s) 62, 80, 132, 153
Bst6I CTCTTC 2 cut(s) 133, 513
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 5 cut(s) 164, 276, 298, 529, 627
BstKTI GATC 1 cut(s) 431
BstMAI GTCTC 2 cut(s) 335, 390
BstMBI GATC 1 cut(s) 428
BstNSI RCATGY 2 cut(s) 229, 540
BstSFI CTRYAG 2 cut(s) 149, 195
BstV1I GCAGC 3 cut(s) 166, 209, 298
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 1 cut(s) 247
BtsCI GGATG 5 cut(s) 164, 276, 298, 529, 627
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 246, 450
CviAII CATG 3 cut(s) 226, 537, 661
CviJI RGCY 3 cut(s) 179, 200, 247
CviKI_1 RGCY 3 cut(s) 179, 200, 247
DdeI CTNAG 1 cut(s) 353
DpnI GATC 1 cut(s) 430
DpnII GATC 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 133, 513
EarI CTCTTC 2 cut(s) 133, 513
Eco130I CCWWGG 2 cut(s) 242, 432
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 246, 450
EcoT14I CCWWGG 2 cut(s) 242, 432
ErhI CCWWGG 2 cut(s) 242, 432
FaeI CATG 3 cut(s) 229, 540, 664
FatI CATG 3 cut(s) 225, 536, 660
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 180, 198, 287
FokI GGATG 5 cut(s) 151, 263, 305, 536, 634
Fsp4HI GCNGC 3 cut(s) 180, 198, 287
FspBI CTAG 1 cut(s) 306
GluI GCNGC 3 cut(s) 180, 198, 287
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 1 cut(s) 247
Hin1II CATG 3 cut(s) 229, 540, 664
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 52, 374
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 5 cut(s) 105, 137, 223, 379, 514
Hpy188III TCNNGA 3 cut(s) 335, 542, 557
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 171
HpyCH4III ACNGT 4 cut(s) 62, 80, 132, 153
HpyCH4V TGCA 6 cut(s) 68, 197, 286, 371, 392, 447
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 3 cut(s) 229, 540, 664
Kzo9I GATC 1 cut(s) 428
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 8 cut(s) 158, 283, 337, 378, 433, 471, 570, 657
Lsp1109I GCAGC 3 cut(s) 166, 209, 298
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 306
MalI GATC 1 cut(s) 430
MboI GATC 1 cut(s) 428
MboII GAAGA 7 cut(s) 53, 150, 434, 501, 527, 530, 557
MflI RGATCY 1 cut(s) 428
MnlI CCTC 8 cut(s) 66, 134, 211, 249, 331, 360, 463, 514
MseI TTAA 1 cut(s) 682
MslI CAYNNNNRTG 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 289
Mva1269I GAATGC 2 cut(s) 31, 665
NdeII GATC 1 cut(s) 428
NlaIII CATG 3 cut(s) 229, 540, 664
NlaIV GGNNCC 2 cut(s) 322, 430
NspI RCATGY 2 cut(s) 229, 540
NspV TTCGAA 1 cut(s) 39
PciI ACATGT 2 cut(s) 225, 536
PctI GAATGC 2 cut(s) 31, 665
PfeI GAWTC 2 cut(s) 52, 374
PkrI GCNGC 3 cut(s) 181, 199, 288
PpuMI RGGWCCY 1 cut(s) 450
PscI ACATGT 2 cut(s) 225, 536
Psp5II RGGWCCY 1 cut(s) 450
PspN4I GGNNCC 2 cut(s) 322, 430
PspPI GGNCC 2 cut(s) 246, 450
PspPPI RGGWCCY 1 cut(s) 450
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 1 cut(s) 682
SatI GCNGC 3 cut(s) 180, 198, 287
Sau3AI GATC 1 cut(s) 428
Sau96I GGNCC 2 cut(s) 246, 450
SetI ASST 3 cut(s) 352, 452, 660
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 2 cut(s) 149, 195
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 333
SsiI CCGC 2 cut(s) 280, 289
SspI AATATT 1 cut(s) 573
SspMI CTAG 1 cut(s) 306
StyI CCWWGG 2 cut(s) 242, 432
TaaI ACNGT 4 cut(s) 62, 80, 132, 153
TaqI TCGA 2 cut(s) 39, 669
TfiI GAWTC 2 cut(s) 52, 374
Tru1I TTAA 1 cut(s) 682
Tru9I TTAA 1 cut(s) 682
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 179, 197, 286
TspDTI ATGAA 4 cut(s) 114, 153, 423, 473
TspGWI ACGGA 2 cut(s) 72, 157
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 450
XceI RCATGY 2 cut(s) 229, 540
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.