Rw2G048080

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006042
Physical Location & Seq
Forward (+)
75500803 .. 75503997
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048080.1

Sequence Viewer

Length: 822 bp
ATGGGCGCTGTCTGTTGTTGTTTGAATGCTGATGATTTTGAAGATTATGTGAATCCAAACAGTTCTGCATTGAGGAACTGTCCATGTCTTGGTTGCTTTTTTCAAAACTTTGTAAATGTGTATTCATCACTGTTCAGAAGAGGGGAACTACAGTCGCTCCCCTCATCCATTCAGGGGGCTGCTTCGATGACTTCTGCAGCCTCACTTGATAGCTCTTTATCTGACATGTATCGCTCTCCTCCAAGGCCCTTGCCTTATGATGCTGACCCCAGATACATCCGTTTGCAGCGGGATGGATTGGTTTCTAGGCGTGAGAAGGGCACTAGTCATTCTCATGAGGAGACTGAACCTCTAAGAAGTGATACTGATGCAGATTCAGAATGTTTGAGTACAGGAGACAAATGGAATGGATCTACTTGTGAAGATGGATCAAAAGAACATCATTCCAGGTCCTCAATGAAGTTGTCATCTGCAAAAGCAACAACCGGATTTGGGATCATTTATACATCTTCAGAAGAAGAGGATGTATGCCCAACATGTCTTGAAGAATATACTCCAGAAAACCCCAAAATAATGACAAAATGCTCTCATCATTATCATCTCGCTTGTATATATGAGTGGATGGAGAGAAGTGAAAGTTGTCCTGTTTGTGGCAAGGTAACATTACCAATGTTTTTCCTTAAGCAGACTTTGTTCATACTCATGAGTCATGATAATATTTCAGCAGTCATAGTTTCTAGTAGGCATGCATCTCTATTATATTGTTATAATATATTTCTGGGATTTCTTAATTTCCCAGATATATCTTATATGGCCAAATAG

Protein Analysis

273

Amino Acids

30.55

Weight (kDa)

5.36

Isoelectric Point (pI)

64.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 172 - 217 2e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 175 - 218 6.4e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 176 - 217 5.1e-11 Ring finger domain
zf-C3HC4_2 PF13923 177 - 217 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 177 - 217 2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 177 - 219 4.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AccB7I CCANNNNNTGG 1 cut(s) 89
AciI CCGC 1 cut(s) 289
AclWI GGATC 3 cut(s) 418, 436, 503
AcoI YGGCCR 1 cut(s) 813
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 2 cut(s) 225, 536
AgsI TTSAA 4 cut(s) 25, 41, 104, 545
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 1 cut(s) 446
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 335, 390
AlwI GGATC 3 cut(s) 418, 436, 503
AoxI GGCC 2 cut(s) 245, 813
ApeKI GCWGC 3 cut(s) 179, 197, 286
AspLEI GCGC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 246, 450
AvaII GGWCC 1 cut(s) 450
BaeGI GKGCMC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BalI TGGCCA 1 cut(s) 815
BbvI GCAGC 3 cut(s) 166, 209, 298
BccI CCATC 3 cut(s) 287, 419, 616
BciT130I CCWGG 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 335, 390
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 3 cut(s) 306, 324, 738
BfmI CTRYAG 2 cut(s) 149, 195
BfoI RGCGCY 1 cut(s) 9
BfrI CTTAAG 1 cut(s) 680
BisI GCNGC 3 cut(s) 180, 198, 287
BlsI GCNGC 3 cut(s) 181, 199, 288
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 2 cut(s) 246, 450
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 3 cut(s) 250, 358, 758
BpmI CTGGAG 1 cut(s) 540
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BseBI CCWGG 1 cut(s) 448
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 5 cut(s) 164, 276, 298, 529, 627
BseLI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BseRI GAGGAG 2 cut(s) 228, 353
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 3 cut(s) 166, 209, 298
BshFI GGCC 2 cut(s) 247, 815
BsiSI CCGG 1 cut(s) 486
BslI CCNNNNNNNGG 4 cut(s) 89, 174, 492, 650
BsmAI GTCTC 2 cut(s) 335, 390
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 2 cut(s) 247, 815
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 3 cut(s) 410, 428, 495
BspACI CCGC 1 cut(s) 289
BspANI GGCC 2 cut(s) 247, 815
BspHI TCATGA 3 cut(s) 334, 702, 709
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 3 cut(s) 418, 436, 503
BspTI CTTAAG 1 cut(s) 680
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 410, 428, 495
BssT1I CCWWGG 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 4 cut(s) 62, 80, 132, 153
Bst6I CTCTTC 2 cut(s) 133, 513
BstAFI CTTAAG 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 747
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 5 cut(s) 164, 276, 298, 529, 627
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 3 cut(s) 413, 431, 498
BstMAI GTCTC 2 cut(s) 335, 390
BstMBI GATC 3 cut(s) 410, 428, 495
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 3 cut(s) 229, 540, 749
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 2 cut(s) 149, 195
BstSLI GKGCMC 1 cut(s) 323
BstV1I GCAGC 3 cut(s) 166, 209, 298
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
BsuRI GGCC 2 cut(s) 247, 815
BtsCI GGATG 5 cut(s) 164, 276, 298, 529, 627
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 747
CciI TCATGA 3 cut(s) 334, 702, 709
CfoI GCGC 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 246, 450
Csp6I GTAC 1 cut(s) 390
CviAII CATG 7 cut(s) 84, 226, 335, 537, 703, 710, 746
CviJI RGCY 5 cut(s) 179, 200, 213, 247, 815
CviKI_1 RGCY 5 cut(s) 179, 200, 213, 247, 815
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 1 cut(s) 353
DpnI GATC 3 cut(s) 412, 430, 497
DpnII GATC 3 cut(s) 410, 428, 495
EaeI YGGCCR 1 cut(s) 813
Eam1104I CTCTTC 2 cut(s) 133, 513
EarI CTCTTC 2 cut(s) 133, 513
Eco130I CCWWGG 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 246, 450
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 7 cut(s) 87, 229, 338, 540, 706, 713, 749
FatI CATG 7 cut(s) 83, 225, 334, 536, 702, 709, 745
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 180, 198, 287
FokI GGATG 5 cut(s) 151, 263, 305, 536, 634
Fsp4HI GCNGC 3 cut(s) 180, 198, 287
FspBI CTAG 3 cut(s) 306, 324, 738
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 180, 198, 287
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 2 cut(s) 247, 815
HapII CCGG 1 cut(s) 486
HhaI GCGC 1 cut(s) 8
Hin1II CATG 7 cut(s) 87, 229, 338, 540, 706, 713, 749
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HinfI GANTC 3 cut(s) 52, 374, 706
HpaII CCGG 1 cut(s) 486
Hpy188I TCNGA 4 cut(s) 137, 223, 379, 514
Hpy188III TCNNGA 5 cut(s) 335, 542, 557, 703, 710
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 4 cut(s) 62, 80, 132, 153
HpyCH4V TGCA 6 cut(s) 68, 197, 286, 371, 473, 749
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 7 cut(s) 87, 229, 338, 540, 706, 713, 749
HspAI GCGC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 410, 428, 495
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 3 cut(s) 166, 209, 298
LweI GCATC 3 cut(s) 250, 358, 758
MaeI CTAG 3 cut(s) 306, 324, 738
MaeIII GTNAC 1 cut(s) 658
MalI GATC 3 cut(s) 412, 430, 497
MboI GATC 3 cut(s) 410, 428, 495
MboII GAAGA 7 cut(s) 53, 150, 434, 501, 527, 530, 557
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 815
MluCI AATT 1 cut(s) 790
MluNI TGGCCA 1 cut(s) 815
MlyI GAGTC 1 cut(s) 715
MnlI CCTC 9 cut(s) 66, 134, 172, 211, 249, 331, 360, 463, 514
Mox20I TGGCCA 1 cut(s) 815
Mph1103I ATGCAT 1 cut(s) 751
MscI TGGCCA 1 cut(s) 815
MseI TTAA 2 cut(s) 681, 789
MslI CAYNNNNRTG 2 cut(s) 333, 701
Msp20I TGGCCA 1 cut(s) 815
MspA1I CMGCKG 1 cut(s) 289
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 1 cut(s) 448
NdeII GATC 3 cut(s) 410, 428, 495
NlaIII CATG 7 cut(s) 87, 229, 338, 540, 706, 713, 749
NsiI ATGCAT 1 cut(s) 751
NspI RCATGY 3 cut(s) 229, 540, 749
PaeI GCATGC 1 cut(s) 749
PagI TCATGA 3 cut(s) 334, 702, 709
PciI ACATGT 2 cut(s) 225, 536
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 52, 374
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 3 cut(s) 181, 199, 288
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
PpuMI RGGWCCY 1 cut(s) 450
PscI ACATGT 2 cut(s) 225, 536
PsiI TTATAA 1 cut(s) 768
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 446
PspGI CCWGG 1 cut(s) 446
PspPI GGNCC 2 cut(s) 246, 450
PspPPI RGGWCCY 1 cut(s) 450
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 2 cut(s) 333, 701
SaqAI TTAA 2 cut(s) 681, 789
SatI GCNGC 3 cut(s) 180, 198, 287
Sau3AI GATC 3 cut(s) 410, 428, 495
Sau96I GGNCC 2 cut(s) 246, 450
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 1 cut(s) 323
SetI ASST 4 cut(s) 215, 352, 452, 660
SfaNI GCATC 3 cut(s) 250, 358, 758
SfcI CTRYAG 2 cut(s) 149, 195
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 333, 701
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
SpeI ACTAGT 1 cut(s) 323
SphI GCATGC 1 cut(s) 749
Sse9I AATT 1 cut(s) 790
SsiI CCGC 1 cut(s) 289
SspI AATATT 1 cut(s) 718
SspMI CTAG 3 cut(s) 306, 324, 738
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 4 cut(s) 62, 80, 132, 153
TaqI TCGA 1 cut(s) 185
TasI AATT 1 cut(s) 790
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 2 cut(s) 52, 374
Tru1I TTAA 2 cut(s) 681, 789
Tru9I TTAA 2 cut(s) 681, 789
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 179, 197, 286
TspDTI ATGAA 3 cut(s) 114, 473, 685
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 135
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 450
XceI RCATGY 3 cut(s) 229, 540, 749
XspI CTAG 3 cut(s) 306, 324, 738
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.