AT5G41600

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007019
Physical Location & Seq
Forward (+)
16636029 .. 16637908
1880 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G41600.1

Sequence Viewer

Length: 774 bp
ATGGTGGAAGACCACAAGCACGAGGAATCGATCTTGGAGAAGATTGTTGAGAAGATCCATGGCCACGGTGACTCGTCTTCTCTGTCCGATTCCGACGACGATAAGAAATCTACATCGTCTTCGTCGTCGTCCTTCAAGTCAAAGATTTACCGACTTTTCGGAAGGGAGAAACCTGTTCACAAGGTTCTCGGTGGTGGAAAACCTGCTGATATATTCCTTTGGAGGAACAAGAAGGTATCTGGTGGAGTCTTGGGTGCTGTAACTGCGTCTTGGGTCTTATTCGAGTTATTCGAATACCATCTCCTCGCTTTTCTCTGTCACTTTGCGATTTTTGCTCTTGCAGCATTGTTCTTGTGGTCTAATGCTTGTACATTCATCCACAAGTCAACTCCTCACATCCCGGAAGTTCACATCCCTGAAGATCCTATTCTTCAACTTGTTTCTGGATTAAGGATTGAAATCAATCGTGGTTTGACCCTTCTTAGGAACATTGCATCAGGAAAAGATGTCAAGAAATTTATCCTGGTAATTGCTGGCTTGTGGGTTTTGTCCATTATTGGCAGCTGCTACAACTTCTTGACATTGTTCTACACTGCTACTGTCCTTCTCTTCACCATTCCTGTACTCTACGAGAAGTATGAGGACAAAGTAGATGCCTATGGGGAGAAGGCGATGAGGGAGATCAAAAAGCAATATGCAGTACTTGATGAGAAGGTATTGCGTAAGGTCATAAGCAAGATTCCAAGAGGAGCTCTGAACAAGAAGAAGGATTAA

Protein Analysis

257

Amino Acids

28.95

Weight (kDa)

9.14

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 69 - 224 1e-56 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 211
AclWI GGATC 2 cut(s) 49, 416
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 515
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 3 cut(s) 370, 624, 702
AfiI CCNNNNNNNGG 1 cut(s) 483
AgsI TTSAA 3 cut(s) 136, 434, 458
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 2 cut(s) 564, 752
AluI AGCT 2 cut(s) 564, 752
Alw21I GWGCWC 1 cut(s) 754
AlwI GGATC 2 cut(s) 49, 416
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 341, 561, 564
ApoI RAATTY 1 cut(s) 515
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 2 cut(s) 80, 604
AsuII TTCGAA 1 cut(s) 291
BalI TGGCCA 1 cut(s) 63
BanII GRGCYC 1 cut(s) 754
BauI CACGAG 1 cut(s) 20
BbsI GAAGAC 3 cut(s) 15, 69, 111
Bbv12I GWGCWC 1 cut(s) 754
BbvI GCAGC 3 cut(s) 353, 551, 573
BccI CCATC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 524
BcnI CCSGG 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 211
BisI GCNGC 3 cut(s) 342, 562, 565
BlsI GCNGC 3 cut(s) 343, 563, 566
BmcAI AGTACT 1 cut(s) 702
Bme1390I CCNGG 2 cut(s) 401, 524
BmrFI CCNGG 2 cut(s) 401, 524
BmsI GCATC 2 cut(s) 503, 643
BpiI GAAGAC 3 cut(s) 15, 69, 111
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 401
Bsa29I ATCGAT 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 58, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 458
BseBI CCWGG 1 cut(s) 524
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 58, 64
BseGI GGATG 3 cut(s) 375, 396, 411
BseJI GATNNNNATC 1 cut(s) 458
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 1 cut(s) 489
BseRI GAGGAG 3 cut(s) 293, 381, 762
BseXI GCAGC 3 cut(s) 353, 551, 573
BshFI GGCC 1 cut(s) 63
BshVI ATCGAT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 754
BsiSI CCGG 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 483
BsnI GGCC 1 cut(s) 63
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 754
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 4 cut(s) 30, 54, 421, 681
Bsp19I CCATGG 1 cut(s) 58
BspANI GGCC 1 cut(s) 63
BspDI ATCGAT 1 cut(s) 29
BspMI ACCTGC 1 cut(s) 211
BspPI GGATC 2 cut(s) 49, 416
BspT104I TTCGAA 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 489
BsrGI TGTACA 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 58, 64
BssMI GATC 4 cut(s) 30, 54, 421, 681
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 2 cut(s) 68, 601
Bst6I CTCTTC 1 cut(s) 614
BstAUI TGTACA 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 1 cut(s) 482
BstDSI CCRYGG 2 cut(s) 58, 64
BstF5I GGATG 3 cut(s) 375, 396, 411
BstKTI GATC 4 cut(s) 33, 57, 424, 684
BstMBI GATC 4 cut(s) 30, 54, 421, 681
BstMWI GCNNNNNNNGC 3 cut(s) 263, 332, 341
BstNI CCWGG 1 cut(s) 524
BstSCI CCNGG 2 cut(s) 399, 522
BstV1I GCAGC 3 cut(s) 353, 551, 573
BstV2I GAAGAC 3 cut(s) 15, 69, 111
BstX2I RGATCY 2 cut(s) 54, 421
BstYI RGATCY 2 cut(s) 54, 421
Bsu15I ATCGAT 1 cut(s) 29
BsuRI GGCC 1 cut(s) 63
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 2 cut(s) 58, 64
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 3 cut(s) 375, 396, 411
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 1 cut(s) 591
BveI ACCTGC 1 cut(s) 211
Cac8I GCNNGC 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 1 cut(s) 255
Csp6I GTAC 3 cut(s) 369, 623, 701
CviAII CATG 1 cut(s) 59
CviJI RGCY 4 cut(s) 63, 537, 564, 752
CviKI_1 RGCY 4 cut(s) 63, 537, 564, 752
CviQI GTAC 3 cut(s) 369, 623, 701
DdeI CTNAG 1 cut(s) 482
DpnI GATC 4 cut(s) 32, 56, 423, 683
DpnII GATC 4 cut(s) 30, 54, 421, 681
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
Ecl136II GAGCTC 1 cut(s) 752
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 754
Eco53kI GAGCTC 1 cut(s) 752
Eco57I CTGAAG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 1 cut(s) 62
FaiI YATR 6 cut(s) 60, 212, 639, 660, 696, 731
FatI CATG 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 342, 562, 565
FokI GGATG 3 cut(s) 362, 383, 398
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 3 cut(s) 342, 562, 565
GluI GCNGC 3 cut(s) 342, 562, 565
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 255
Hin1II CATG 1 cut(s) 62
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 5 cut(s) 26, 71, 89, 246, 739
HpaII CCGG 1 cut(s) 401
HphI GGTGA 2 cut(s) 80, 604
Hpy166II GTNNAC 3 cut(s) 178, 387, 409
Hpy188I TCNGA 4 cut(s) 88, 94, 161, 756
Hpy188III TCNNGA 4 cut(s) 444, 498, 511, 577
Hpy8I GTNNAC 3 cut(s) 178, 387, 409
Hpy99I CGWCG 4 cut(s) 98, 101, 127, 130
HpyAV CCTTC 8 cut(s) 142, 156, 226, 488, 614, 661, 706, 760
HpyCH4III ACNGT 2 cut(s) 68, 601
HpyCH4V TGCA 3 cut(s) 341, 494, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 332, 341
HpyF3I CTNAG 1 cut(s) 482
Hsp92II CATG 1 cut(s) 62
Kzo9I GATC 4 cut(s) 30, 54, 421, 681
LmnI GCTCC 1 cut(s) 749
Lsp1109I GCAGC 3 cut(s) 353, 551, 573
LweI GCATC 2 cut(s) 503, 643
MaeIII GTNAC 3 cut(s) 68, 259, 317
MalI GATC 4 cut(s) 32, 56, 423, 683
MboI GATC 4 cut(s) 30, 54, 421, 681
MboII GAAGA 8 cut(s) 20, 52, 64, 69, 111, 422, 431, 601
MflI RGATCY 2 cut(s) 54, 421
MhlI GDGCHC 1 cut(s) 754
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 2 cut(s) 515, 528
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 2 cut(s) 65, 255
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 7 cut(s) 16, 216, 314, 402, 634, 669, 740
Mox20I TGGCCA 1 cut(s) 63
MscI TGGCCA 1 cut(s) 63
MseI TTAA 2 cut(s) 449, 772
Msp20I TGGCCA 1 cut(s) 63
MspA1I CMGCKG 1 cut(s) 564
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 2 cut(s) 401, 524
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 263, 332, 341
NciI CCSGG 1 cut(s) 401
NcoI CCATGG 1 cut(s) 58
NdeII GATC 4 cut(s) 30, 54, 421, 681
NlaIII CATG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 68, 317
NspV TTCGAA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 288
PfeI GAWTC 3 cut(s) 26, 89, 739
PfoI TCCNGGA 1 cut(s) 399
PkrI GCNGC 3 cut(s) 343, 563, 566
PleI GAGTC 2 cut(s) 65, 254
PpsI GAGTC 2 cut(s) 65, 254
Psp124BI GAGCTC 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PsuI RGATCY 2 cut(s) 54, 421
PvuII CAGCTG 1 cut(s) 564
RsaI GTAC 3 cut(s) 370, 624, 702
RsaNI GTAC 3 cut(s) 369, 623, 701
SacI GAGCTC 1 cut(s) 754
SaqAI TTAA 2 cut(s) 449, 772
SatI GCNGC 3 cut(s) 342, 562, 565
Sau3AI GATC 4 cut(s) 30, 54, 421, 681
ScaI AGTACT 1 cut(s) 702
SchI GAGTC 2 cut(s) 65, 255
ScrFI CCNGG 2 cut(s) 401, 524
SduI GDGCHC 1 cut(s) 754
SetI ASST 8 cut(s) 175, 186, 205, 237, 566, 717, 729, 754
SfaNI GCATC 2 cut(s) 503, 643
SfuI TTCGAA 1 cut(s) 291
Sse9I AATT 2 cut(s) 515, 528
SstI GAGCTC 1 cut(s) 754
StyD4I CCNGG 2 cut(s) 399, 522
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 2 cut(s) 68, 601
TaqI TCGA 3 cut(s) 29, 282, 291
TasI AATT 2 cut(s) 515, 528
TatI WGTACW 3 cut(s) 368, 622, 700
TfiI GAWTC 3 cut(s) 26, 89, 739
Tru1I TTAA 2 cut(s) 449, 772
Tru9I TTAA 2 cut(s) 449, 772
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 68, 317
TseI GCWGC 3 cut(s) 341, 561, 564
Tsp45I GTSAC 2 cut(s) 68, 317
TspDTI ATGAA 1 cut(s) 364
TspRI CASTG 1 cut(s) 598
XapI RAATTY 1 cut(s) 515
ZrmI AGTACT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.