Rh1AG434700

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007019
Physical Location & Seq
Forward (+)
66011535 .. 66013546
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG434700.1

Sequence Viewer

Length: 819 bp
ATGGCGGAGCCAGAAGAGATACCAGGGGCGTCGCTCATGGAGAAGATCTACTCCCACCACCGCTCCTCGTCGTCTTCGTCGTCTTCCGACGACGACAAGCAGTCCAAGAAGGCCGATGCCGTCAAGAAGGCCGATGACGTCAAGCACAAGGCTGAGGAACACGACATGCCGTCCGCTCACGATTCCGTCAAGTCCAACAAATTCAGGATCTTCGGCCGGGAAAAGCCCGTTCATAAGGTCCTCGGCGGTGGCAAACCTGCTGATGTCCTCCTATGGAGGGACAAGAAAGTATCTGGAAGTGTTCTTGGTGGAGCCACTGTGCTATGGATTTTCTTTGAGTTGCTTGAATACCATTTGATAACTCTGGTCTGCCATATGTTGATACTTTCTCTGGGCGTTTTCTTCCTGTGGTCCAATGCATCCTCCATTATCAACAAGGCTCCACCAGAAATCCCAAAAGTTCAAATTCCGGAGAAGTGTGTCCTTGATCTTGCCTCTGCACTGAGGATTGAACTCAACAAAGGGTTCTATGTTCTCCGGGAAATTGCATCTGGGAGAGATTTGAGGACATTCCTCATTGTTATAGTTGTCTTGTGGATTGTGTCAGTGATTGGCAAATGGTTCAACTTCCTGACCTTATTCTACTTATCCTTTGTCTTGCTGCACACGGTGCCAGTGATCTATGAGAAACATGATGTCCAGATTGATGCTTTTGCTGAGAAGGCATGGATTGAGATTAAGAAGCAGTATGCAGTGTTTGATGCGAATGTTTTGAGTAAGATACCCAAGGGTCCAGTGAAAGAGAAGAAGAAGGACTAG

Protein Analysis

272

Amino Acids

30.67

Weight (kDa)

8.97

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 87 - 242 5.4e-57 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 670
AccBSI CCGCTC 2 cut(s) 63, 176
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 4 cut(s) 5, 61, 174, 246
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 2 cut(s) 200, 465
AcyI GRCGYC 2 cut(s) 29, 138
AdeI CACNNNGTG 1 cut(s) 670
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 4 cut(s) 347, 464, 512, 625
AhdI GACNNNNNGTC 2 cut(s) 100, 169
AjnI CCWGG 1 cut(s) 22
AlwI GGATC 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 3 cut(s) 111, 129, 214
ApeKI GCWGC 1 cut(s) 661
ApoI RAATTY 2 cut(s) 200, 465
AspS9I GGNCC 3 cut(s) 238, 411, 791
AsuC2I CCSGG 2 cut(s) 218, 539
AvaII GGWCC 3 cut(s) 238, 411, 791
BanI GGYRCC 1 cut(s) 670
BbsI GAAGAC 2 cut(s) 66, 75
BbvCI CCTCAGC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 648
BceAI ACGGC 2 cut(s) 104, 154
BciT130I CCWGG 1 cut(s) 24
BcnI CCSGG 2 cut(s) 218, 539
BfaI CTAG 1 cut(s) 817
BfuAI ACCTGC 1 cut(s) 265
BglII AGATCT 1 cut(s) 45
BisI GCNGC 1 cut(s) 662
BlsI GCNGC 1 cut(s) 663
Bme1390I CCNGG 3 cut(s) 24, 218, 539
Bme18I GGWCC 3 cut(s) 238, 411, 791
BmeRI GACNNNNNGTC 2 cut(s) 100, 169
BmgT120I GGNCC 3 cut(s) 238, 411, 791
BmiI GGNNCC 5 cut(s) 9, 313, 441, 672, 792
BmrFI CCNGG 3 cut(s) 24, 218, 539
BmsI GCATC 5 cut(s) 106, 428, 557, 697, 751
BpiI GAAGAC 2 cut(s) 66, 75
Bpu10I CCTNAGC 1 cut(s) 153
BpuMI CCSGG 2 cut(s) 218, 539
BsaHI GRCGYC 2 cut(s) 29, 138
BsaJI CCNNGG 3 cut(s) 23, 241, 786
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 303, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 674, 794
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 23, 241, 786
BseGI GGATG 1 cut(s) 419
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 3 cut(s) 144, 494, 708
BseNI ACTGG 2 cut(s) 674, 794
BseRI GAGGAG 1 cut(s) 55
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 648
BsgI GTGCAG 2 cut(s) 483, 647
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 3 cut(s) 113, 131, 216
BshNI GGYRCC 1 cut(s) 670
BsiEI CGRYCG 1 cut(s) 217
BsiSI CCGG 3 cut(s) 217, 470, 538
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 3 cut(s) 113, 131, 216
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 4 cut(s) 45, 207, 487, 678
BspACI CCGC 4 cut(s) 5, 61, 174, 246
BspANI GGCC 3 cut(s) 113, 131, 216
BspCNI CTCAG 3 cut(s) 145, 495, 709
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 5 cut(s) 9, 313, 441, 672, 792
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 670
BsrBI CCGCTC 2 cut(s) 63, 176
BsrI ACTGG 2 cut(s) 674, 794
BssECI CCNNGG 3 cut(s) 23, 241, 786
BssMI GATC 4 cut(s) 45, 207, 487, 678
BssNI GRCGYC 2 cut(s) 29, 138
BssT1I CCWWGG 1 cut(s) 786
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 319, 670
Bst6I CTCTTC 1 cut(s) 9
BstACI GRCGYC 2 cut(s) 29, 138
BstAPI GCANNNNNTGC 1 cut(s) 670
BstDEI CTNAG 3 cut(s) 153, 503, 717
BstF5I GGATG 1 cut(s) 419
BstKTI GATC 4 cut(s) 48, 210, 490, 681
BstMBI GATC 4 cut(s) 45, 207, 487, 678
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 670, 722
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 3 cut(s) 22, 216, 537
BstV1I GCAGC 1 cut(s) 648
BstV2I GAAGAC 2 cut(s) 66, 75
BstX2I RGATCY 2 cut(s) 45, 207
BstYI RGATCY 2 cut(s) 45, 207
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 3 cut(s) 113, 131, 216
BtsCI GGATG 1 cut(s) 419
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 6 cut(s) 315, 500, 612, 681, 759, 801
BveI ACCTGC 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 238, 411, 791
CseI GACGC 1 cut(s) 18
CviAII CATG 4 cut(s) 37, 166, 692, 726
CviJI RGCY 8 cut(s) 10, 113, 131, 152, 216, 226, 314, 440
CviKI_1 RGCY 8 cut(s) 10, 113, 131, 152, 216, 226, 314, 440
DdeI CTNAG 3 cut(s) 153, 503, 717
DpnI GATC 4 cut(s) 47, 209, 489, 680
DpnII GATC 4 cut(s) 45, 207, 487, 678
DraIII CACNNNGTG 1 cut(s) 670
DriI GACNNNNNGTC 2 cut(s) 100, 169
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 2 cut(s) 100, 169
EarI CTCTTC 1 cut(s) 9
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 786
Eco47I GGWCC 3 cut(s) 238, 411, 791
Eco52I CGGCCG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 786
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 786
FaeI CATG 4 cut(s) 40, 169, 695, 729
FaqI GGGAC 1 cut(s) 293
FatI CATG 4 cut(s) 36, 165, 691, 725
FauNDI CATATG 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 662
FokI GGATG 1 cut(s) 406
Fsp4HI GCNGC 1 cut(s) 662
FspBI CTAG 1 cut(s) 817
GluI GCNGC 1 cut(s) 662
HaeIII GGCC 3 cut(s) 113, 131, 216
HapII CCGG 3 cut(s) 217, 470, 538
HgaI GACGC 1 cut(s) 18
Hin1I GRCGYC 2 cut(s) 29, 138
Hin1II CATG 4 cut(s) 40, 169, 695, 729
HinfI GANTC 1 cut(s) 182
HpaII CCGG 3 cut(s) 217, 470, 538
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 7 cut(s) 124, 179, 205, 294, 470, 631, 700
Hpy99I CGWCG 5 cut(s) 34, 73, 82, 92, 95
HpyAV CCTTC 4 cut(s) 103, 121, 715, 805
HpyCH4III ACNGT 2 cut(s) 319, 670
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 5 cut(s) 419, 500, 548, 664, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 670, 722
HpyF3I CTNAG 3 cut(s) 153, 503, 717
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 2 cut(s) 29, 138
Hsp92II CATG 4 cut(s) 40, 169, 695, 729
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 4 cut(s) 45, 207, 487, 678
LmnI GCTCC 4 cut(s) 7, 68, 311, 445
Lsp1109I GCAGC 1 cut(s) 648
LweI GCATC 5 cut(s) 106, 428, 557, 697, 751
MaeI CTAG 1 cut(s) 817
MaeII ACGT 1 cut(s) 138
MalI GATC 4 cut(s) 47, 209, 489, 680
MbiI CCGCTC 2 cut(s) 63, 176
MboI GATC 4 cut(s) 45, 207, 487, 678
MboII GAAGA 7 cut(s) 26, 55, 66, 75, 202, 394, 817
MflI RGATCY 2 cut(s) 45, 207
MluCI AATT 3 cut(s) 200, 465, 543
MmeI TCCRAC 2 cut(s) 111, 219
Mph1103I ATGCAT 1 cut(s) 421
MroI TCCGGA 1 cut(s) 469
MseI TTAA 1 cut(s) 738
MspI CCGG 3 cut(s) 217, 470, 538
MspR9I CCNGG 3 cut(s) 24, 218, 539
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 670, 722
NciI CCSGG 2 cut(s) 218, 539
NdeI CATATG 1 cut(s) 375
NdeII GATC 4 cut(s) 45, 207, 487, 678
NlaIII CATG 4 cut(s) 40, 169, 695, 729
NlaIV GGNNCC 5 cut(s) 9, 313, 441, 672, 792
NmeAIII GCCGAG 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 1 cut(s) 169
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 77
PfeI GAWTC 1 cut(s) 182
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 1 cut(s) 663
PpuMI RGGWCCY 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 5 cut(s) 9, 313, 441, 672, 792
PspPI GGNCC 3 cut(s) 238, 411, 791
PspPPI RGGWCCY 1 cut(s) 238
PsuI RGATCY 2 cut(s) 45, 207
SaqAI TTAA 1 cut(s) 738
SatI GCNGC 1 cut(s) 662
Sau3AI GATC 4 cut(s) 45, 207, 487, 678
Sau96I GGNCC 3 cut(s) 238, 411, 791
ScrFI CCNGG 3 cut(s) 24, 218, 539
SetI ASST 4 cut(s) 141, 240, 259, 638
SfaNI GCATC 5 cut(s) 106, 428, 557, 697, 751
SinI GGWCC 3 cut(s) 238, 411, 791
Sse9I AATT 3 cut(s) 200, 465, 543
SsiI CCGC 4 cut(s) 5, 61, 174, 246
SspMI CTAG 1 cut(s) 817
StyD4I CCNGG 3 cut(s) 22, 216, 537
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 2 cut(s) 319, 670
TaiI ACGT 1 cut(s) 141
TasI AATT 3 cut(s) 200, 465, 543
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 6 cut(s) 322, 507, 612, 681, 759, 801
TseI GCWGC 1 cut(s) 661
TspDTI ATGAA 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 6 cut(s) 322, 507, 612, 681, 759, 801
VpaK11BI GGWCC 3 cut(s) 238, 411, 791
XapI RAATTY 2 cut(s) 200, 465
XceI RCATGY 1 cut(s) 169
XspI CTAG 1 cut(s) 817
ZraI GACGTC 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.