MD01G1211100.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007019
Physical Location & Seq
Forward (+)
30480322 .. 30482525
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1211100.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGACAAGATCTACAACCACCACTCTTCGTCGTCGTCGGACTCCGACGACGACAAGAAGTCCAAGCACGCTGCTTCCGTCAAAGCCAAGGTTGAAGACGAGAAGCCGTCGGCGCGCGATGCCGTCAAATCCAAGATTTTCAGGCTCTTCGGCAGGGAAAAGCCCGTTCACAAGGTCTTGGGCGGCGGCAAACCTGCTGATGTTCTCCTATGGAGGAACAAGAAAATATCTGCAAGTGTGCTTGCCGGTGCCACTGTTTTATGGGTTTTCTTTGAGTTGCTCGAATACCATCTAATCACGCTGGTGTGCCATTTGTTGATACTTTCTCTGGCTGTCTTTTACCTGTGGTCCAATGCATCCAACTTTATCAACAAGTCGCAACCACAAATCCCAAAAGTTCAAATTCCTGAGAAGACCGTCCTTGAGCTTGCCTCTGCTCTGAGAATTGAACTCAACAAAGGATTTTATGTTTTGCGGAATATTGCATCCGGGAGAGATTTGAAGGCATTCCTTGGTGTTATAGCCGTCTTGTGGATTCTGTCGGTGGTTGGGAAATGGTTCAACTTTCTGACCTTGTTCTACATAATTTTTGTGTTGCTGCACACGCTGCCCGTGATTTATGAGAAATACGATGACCAGATTGATGCCTTTGGCGAGAAGGCGTGGATCGAAATAAAGAAGCAGTACGCAGTGTTTGATGCCAAGGTTTTGAGTAAAATCCCCAAGGGTCCAGTGAAGGAGAAGAAGAAGGATTAG

Protein Analysis

252

Amino Acids

28.47

Weight (kDa)

9.55

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 66 - 220 1.3e-58 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 248
AccII CGCG 2 cut(s) 115, 117
AciI CCGC 3 cut(s) 183, 186, 475
AclWI GGATC 1 cut(s) 674
AcsI RAATTY 1 cut(s) 402
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 1 cut(s) 531
AgsI TTSAA 5 cut(s) 95, 401, 449, 502, 562
AhdI GACNNNNNGTC 1 cut(s) 58
AleI CACNNNNGTG 1 cut(s) 302
AluBI AGCT 1 cut(s) 427
AluI AGCT 1 cut(s) 427
AlwI GGATC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 71, 598, 607
ApoI RAATTY 1 cut(s) 402
Asp700I GAANNNNTTC 2 cut(s) 506, 557
AspLEI GCGC 2 cut(s) 115, 117
AspS9I GGNCC 2 cut(s) 348, 728
AsuC2I CCSGG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 348, 728
BanI GGYRCC 1 cut(s) 248
BbsI GAAGAC 2 cut(s) 102, 419
BbvI GCAGC 3 cut(s) 58, 585, 594
BccI CCATC 1 cut(s) 297
BceAI ACGGC 3 cut(s) 91, 107, 509
BcnI CCSGG 1 cut(s) 490
BfuAI ACCTGC 1 cut(s) 202
BglII AGATCT 1 cut(s) 9
BisI GCNGC 5 cut(s) 72, 184, 187, 599, 608
BlsI GCNGC 5 cut(s) 73, 185, 188, 600, 609
Bme1390I CCNGG 1 cut(s) 490
Bme18I GGWCC 2 cut(s) 348, 728
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 348, 728
BmiI GGNNCC 2 cut(s) 250, 729
BmrFI CCNGG 1 cut(s) 490
BmsI GCATC 5 cut(s) 109, 365, 494, 634, 688
BpiI GAAGAC 2 cut(s) 102, 419
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 490
BsaJI CCNNGG 4 cut(s) 87, 511, 702, 723
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 731
BseDI CCNNGG 4 cut(s) 87, 511, 702, 723
BseGI GGATG 2 cut(s) 356, 485
BseLI CCNNNNNNNGG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 399, 431
BseNI ACTGG 1 cut(s) 731
BsePI GCGCGC 1 cut(s) 113
BseXI GCAGC 3 cut(s) 58, 585, 594
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 2 cut(s) 115, 117
BshNI GGYRCC 1 cut(s) 248
BsiSI CCGG 2 cut(s) 246, 489
BslI CCNNNNNNNGG 1 cut(s) 531
BsmI GAATGC 1 cut(s) 506
Bsp143I GATC 2 cut(s) 9, 666
BspACI CCGC 3 cut(s) 183, 186, 475
BspCNI CTCAG 2 cut(s) 400, 432
BspFNI CGCG 2 cut(s) 115, 117
BspLI GGNNCC 2 cut(s) 250, 729
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 1 cut(s) 674
BspQI GCTCTTC 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 1 cut(s) 731
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 4 cut(s) 87, 511, 702, 723
BssHII GCGCGC 1 cut(s) 113
BssMI GATC 2 cut(s) 9, 666
BssT1I CCWWGG 4 cut(s) 87, 511, 702, 723
Bst4CI ACNGT 2 cut(s) 256, 418
Bst6I CTCTTC 2 cut(s) 31, 152
BstAPI GCANNNNNTGC 1 cut(s) 607
BstC8I GCNNGC 4 cut(s) 69, 115, 243, 429
BstDEI CTNAG 2 cut(s) 408, 440
BstF5I GGATG 2 cut(s) 356, 485
BstFNI CGCG 2 cut(s) 115, 117
BstHHI GCGC 2 cut(s) 115, 117
BstKTI GATC 2 cut(s) 12, 669
BstMBI GATC 2 cut(s) 9, 666
BstMWI GCNNNNNNNGC 4 cut(s) 112, 119, 604, 607
BstSCI CCNGG 1 cut(s) 488
BstUI CGCG 2 cut(s) 115, 117
BstV1I GCAGC 3 cut(s) 58, 585, 594
BstV2I GAAGAC 2 cut(s) 102, 419
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 356, 485
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 252, 696, 738
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 4 cut(s) 69, 115, 243, 429
CfoI GCGC 2 cut(s) 115, 117
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 348, 728
Csp6I GTAC 1 cut(s) 685
CviJI RGCY 7 cut(s) 86, 106, 145, 163, 332, 427, 524
CviKI_1 RGCY 7 cut(s) 86, 106, 145, 163, 332, 427, 524
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 2 cut(s) 408, 440
DpnI GATC 2 cut(s) 11, 668
DpnII GATC 2 cut(s) 9, 666
DriI GACNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 31, 152
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 2 cut(s) 31, 152
Eco130I CCWWGG 4 cut(s) 87, 511, 702, 723
Eco47I GGWCC 2 cut(s) 348, 728
EcoT14I CCWWGG 4 cut(s) 87, 511, 702, 723
EcoT22I ATGCAT 1 cut(s) 358
ErhI CCWWGG 4 cut(s) 87, 511, 702, 723
FaiI YATR 6 cut(s) 211, 262, 468, 521, 584, 621
Fnu4HI GCNGC 5 cut(s) 72, 184, 187, 599, 608
FokI GGATG 2 cut(s) 343, 472
Fsp4HI GCNGC 5 cut(s) 72, 184, 187, 599, 608
GlaI GCGC 2 cut(s) 114, 116
GluI GCNGC 5 cut(s) 72, 184, 187, 599, 608
HapII CCGG 2 cut(s) 246, 489
HhaI GCGC 2 cut(s) 115, 117
Hin6I GCGC 2 cut(s) 113, 115
HinP1I GCGC 2 cut(s) 113, 115
HinfI GANTC 2 cut(s) 41, 535
HpaII CCGG 2 cut(s) 246, 489
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 4 cut(s) 40, 46, 441, 570
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 6 cut(s) 34, 37, 40, 50, 53, 112
HpyAV CCTTC 4 cut(s) 496, 652, 730, 742
HpyCH4III ACNGT 2 cut(s) 256, 418
HpyCH4V TGCA 4 cut(s) 233, 356, 485, 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 119, 604, 607
HpyF3I CTNAG 2 cut(s) 408, 440
HspAI GCGC 2 cut(s) 113, 115
Kzo9I GATC 2 cut(s) 9, 666
LguI GCTCTTC 1 cut(s) 152
Lsp1109I GCAGC 3 cut(s) 58, 585, 594
LweI GCATC 5 cut(s) 109, 365, 494, 634, 688
MalI GATC 2 cut(s) 11, 668
MboI GATC 2 cut(s) 9, 666
MboII GAAGA 5 cut(s) 18, 107, 139, 424, 754
MflI RGATCY 1 cut(s) 9
MluCI AATT 3 cut(s) 402, 444, 585
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 3 cut(s) 18, 69, 384
MnlI CCTC 2 cut(s) 207, 442
Mph1103I ATGCAT 1 cut(s) 358
MroXI GAANNNNTTC 2 cut(s) 506, 557
MslI CAYNNNNRTG 1 cut(s) 302
MspI CCGG 2 cut(s) 246, 489
MspR9I CCNGG 1 cut(s) 490
Mva1269I GAATGC 1 cut(s) 506
MvnI CGCG 2 cut(s) 115, 117
MwoI GCNNNNNNNGC 4 cut(s) 112, 119, 604, 607
NciI CCSGG 1 cut(s) 490
NdeII GATC 2 cut(s) 9, 666
NlaIV GGNNCC 2 cut(s) 250, 729
NsiI ATGCAT 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 302
PauI GCGCGC 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 506
PdmI GAANNNNTTC 2 cut(s) 506, 557
PfeI GAWTC 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 5 cut(s) 73, 185, 188, 600, 609
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PspN4I GGNNCC 2 cut(s) 250, 729
PspPI GGNCC 2 cut(s) 348, 728
PsuI RGATCY 1 cut(s) 9
PteI GCGCGC 1 cut(s) 113
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 5 cut(s) 72, 184, 187, 599, 608
Sau3AI GATC 2 cut(s) 9, 666
Sau96I GGNCC 2 cut(s) 348, 728
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 490
SetI ASST 7 cut(s) 93, 177, 196, 345, 429, 575, 708
SfaNI GCATC 5 cut(s) 109, 365, 494, 634, 688
SinI GGWCC 2 cut(s) 348, 728
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 3 cut(s) 402, 444, 585
SsiI CCGC 3 cut(s) 183, 186, 475
SspI AATATT 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 488
StyI CCWWGG 4 cut(s) 87, 511, 702, 723
TaaI ACNGT 2 cut(s) 256, 418
TaqI TCGA 2 cut(s) 282, 669
TasI AATT 3 cut(s) 402, 444, 585
TauI GCSGC 2 cut(s) 186, 189
TfiI GAWTC 1 cut(s) 535
TscAI CASTG 3 cut(s) 259, 696, 738
TseI GCWGC 3 cut(s) 71, 598, 607
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 3 cut(s) 259, 696, 738
VpaK11BI GGWCC 2 cut(s) 348, 728
XapI RAATTY 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 506, 557
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.