AT5G47360

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008335
Physical Location & Seq
Reverse (-)
19214445 .. 19215878
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47360.1

Sequence Viewer

Length: 1434 bp
ATGCCAAATTCATTGATTTCTCGTCTGGTATCTCCTTCATTACGATCTCAGCCCTCAAAAATCTCAGCTTTACGTTTTTTAACAACTGTCTCAGCCGCCGAGAGATTATACGGCCAGCTTCAAGGATGCACAAGTAATCTCGAGAAAGAATTAGCTTCGGCTAATGTACAATTAGACTCCTCTTGTATCAACGAGGTACTAAGAAGATGCGATCCAAATCAATTTCAATCGGGTCTTAGGTTTTTCATATGGGCAGGAACTCTATCCAGCCATAGACACAGTGCTTACATGTATACCAAAGCTTGCGACATTCTTAAGATTAGAGCAAAGCCAGATTTGATCAAATACGTTATTGAATCTTATCGGAAAGAAGAATGCTTTGTTAATGTTAAGACGATGAGGATTGTTCTGACTCTGTGTAATCAAGCGAATCTCGCTGATGAGGCATTGTGGGTATTAAGGAAGTTTCCTGAATTTAATGTATGTGCAGACACTGTTGCGTATAATTTGGTGATTAGGTTGTTTGCTGATAAGGGAGATTTGAATATAGCTGATATGTTGATAAAAGAGATGGATTGTGTTGGTCTTTATCCAGATGTGATTACTTATACGTCGATGATCAATGGGTATTGCAATGCGGGTAAGATTGATGACGCTTGGAGGTTAGCTAAGGAGATGAGTAAGCATGATTGCGTGCTTAACAGTGTGACGTATTCTAGGATTCTTGAAGGAGTTTGCAAGTCTGGTGATATGGAGAGAGCGTTAGAGTTGTTGGCGGAGATGGAGAAAGAAGACGGTGGTGGATTGATTAGTCCGAATGCGGTTACTTATACTTTGGTGATTCAAGCGTTTTGTGAGAAGAGGAGGGTTGAAGAGGCGTTGTTGGTATTGGATAGAATGGGAAACCGAGGGTGCATGCCGAATCGTGTTACTGCTTGTGTTTTGATTCAGGGGGTTTTGGAGAATGATGAGGATGTGAAGGCTCTTTCTAAGTTGATTGATAAGTTGGTGAAACTAGGCGGTGTTTCTCTTTCTGAGTGTTTTAGTTCGGCTACTGTGTCGCTAATCCGTATGAAAAGATGGGAAGAGGCTGAAAAGATTTTCCGTCTGATGTTGGTCCGCGGGGTTAGACCTGATGGTCTTGCGTGCAGTCATGTTTTCAGGGAGTTATGTTTGTTGGAAAGGTATCTCGATTGTTTCCTTCTGTATCAAGAAATCGAGAAGAAGGATGTGAAATCAACAATAGACTCGGATATACATGCGGTTCTTTTGCTCGGTCTCTGCCAACAGGGGAATTCTTGGGAAGCTGCTAAGCTTGCGAAATCAATGCTTGACAAGAAAATGAGATTGAAAGTTTCTCATGTTGAGAAAATCATTGAAGCCTTGAAGAAAACTGGTGATGAAGATCTCATGAGTCGTTTCTCTATTGATTAA

Protein Analysis

477

Amino Acids

53.78

Weight (kDa)

7.39

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 122 - 243 6.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 164 - 212 1.3e-13 PPR repeat family
PPR_3 PF13812 187 - 242 2.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 195 - 227 3.7e-13 PPR repeat
PPR_2 PF13041 198 - 247 5.7e-17 PPR repeat family
PPR PF01535 201 - 231 8.6e-08 PPR repeat
PPR_1 PF12854 230 - 262 1.7e-07 PPR repeat
PPR_2 PF13041 234 - 264 5.8e-06 PPR repeat family
PPR_1 PF12854 270 - 300 8e-08 PPR repeat
PPR_2 PF13041 272 - 319 1.5e-12 PPR repeat family
PPR PF01535 275 - 305 3.3e-06 PPR repeat
TPR_24 PF23276 275 - 339 2.7e-06 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1137
AccI GTMKAC 1 cut(s) 293
AccII CGCG 1 cut(s) 1122
AciI CCGC 8 cut(s) 96, 638, 776, 821, 1020, 1120, 1122, 1262
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 3 cut(s) 7, 473, 1294
AfaI GTAC 2 cut(s) 168, 198
AflII CTTAAG 1 cut(s) 314
AflIII ACRYGT 1 cut(s) 288
AluBI AGCT 8 cut(s) 68, 118, 155, 302, 551, 668, 1307, 1315
AluI AGCT 8 cut(s) 68, 118, 155, 302, 551, 668, 1307, 1315
Alw26I GTCTC 2 cut(s) 94, 1283
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 494
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 1 cut(s) 1307
ApoI RAATTY 3 cut(s) 7, 473, 1294
AspS9I GGNCC 1 cut(s) 1117
AsuHPI GGTGA 5 cut(s) 523, 758, 850, 1021, 1409
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 1117
BbsI GAAGAC 1 cut(s) 798
BbvI GCAGC 1 cut(s) 1294
BccI CCATC 4 cut(s) 565, 775, 1074, 1130
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 1309, 1343
BclI TGATCA 2 cut(s) 339, 618
BcoDI GTCTC 2 cut(s) 94, 1283
BfaI CTAG 2 cut(s) 717, 1016
BfrI CTTAAG 1 cut(s) 314
BglII AGATCT 1 cut(s) 1405
BisI GCNGC 2 cut(s) 96, 1308
BlpI GCTNAGC 1 cut(s) 1311
BlsI GCNGC 2 cut(s) 97, 1309
Bme18I GGWCC 1 cut(s) 1117
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 1117
BmsI GCATC 2 cut(s) 116, 197
BpiI GAAGAC 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 669
Bpu1102I GCTNAGC 1 cut(s) 1311
BsaBI GATNNNNATC 2 cut(s) 216, 1404
BsaI GGTCTC 1 cut(s) 1283
BsaJI CCNNGG 2 cut(s) 907, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bse1I ACTGG 1 cut(s) 1399
Bse3DI GCAATG 1 cut(s) 640
Bse8I GATNNNNATC 2 cut(s) 216, 1404
BseDI CCNNGG 2 cut(s) 907, 1120
BseGI GGATG 3 cut(s) 131, 979, 1234
BseJI GATNNNNATC 2 cut(s) 216, 1404
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 4 cut(s) 62, 78, 105, 1026
BseNI ACTGG 1 cut(s) 1399
BseRI GAGGAG 2 cut(s) 169, 877
BseXI GCAGC 1 cut(s) 1294
BsgI GTGCAG 2 cut(s) 507, 1168
Bsh1236I CGCG 1 cut(s) 1122
BshFI GGCC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 140
BsmAI GTCTC 2 cut(s) 94, 1283
BsmI GAATGC 2 cut(s) 380, 823
BsnI GGCC 1 cut(s) 114
Bso31I GGTCTC 1 cut(s) 1283
BsoBI CYCGRG 1 cut(s) 140
Bsp1407I TGTACA 1 cut(s) 166
Bsp143I GATC 5 cut(s) 44, 211, 339, 618, 1405
Bsp1720I GCTNAGC 1 cut(s) 1311
BspACI CCGC 8 cut(s) 96, 638, 776, 821, 1020, 1120, 1122, 1262
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 4 cut(s) 61, 77, 104, 1027
BspFNI CGCG 1 cut(s) 1122
BspHI TCATGA 1 cut(s) 1410
BspPI GGATC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 1283
BsrDI GCAATG 1 cut(s) 640
BsrGI TGTACA 1 cut(s) 166
BsrI ACTGG 1 cut(s) 1399
BssECI CCNNGG 2 cut(s) 907, 1120
BssMI GATC 5 cut(s) 44, 211, 339, 618, 1405
BssNAI GTATAC 1 cut(s) 294
Bst1107I GTATAC 1 cut(s) 294
Bst4CI ACNGT 6 cut(s) 88, 281, 496, 704, 797, 1057
Bst6I CTCTTC 3 cut(s) 854, 867, 1080
BstAFI CTTAAG 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 166
BstC8I GCNNGC 6 cut(s) 116, 304, 695, 917, 1147, 1317
BstDEI CTNAG 9 cut(s) 48, 64, 91, 200, 236, 669, 990, 1035, 1311
BstDSI CCRYGG 1 cut(s) 1120
BstF5I GGATG 3 cut(s) 131, 979, 1234
BstFNI CGCG 1 cut(s) 1122
BstKTI GATC 5 cut(s) 47, 214, 342, 621, 1408
BstMAI GTCTC 2 cut(s) 94, 1283
BstMBI GATC 5 cut(s) 44, 211, 339, 618, 1405
BstMWI GCNNNNNNNGC 3 cut(s) 434, 443, 1316
BstNSI RCATGY 3 cut(s) 292, 919, 1262
BstUI CGCG 1 cut(s) 1122
BstV1I GCAGC 1 cut(s) 1294
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 1405
BstYI RGATCY 1 cut(s) 1405
BstZ17I GTATAC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 1120
BtsCI GGATG 3 cut(s) 131, 979, 1234
BtsIMutI CAGTG 3 cut(s) 286, 492, 709
Cac8I GCNNGC 6 cut(s) 116, 304, 695, 917, 1147, 1317
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 1410
Cfr13I GGNCC 1 cut(s) 1117
Cfr42I CCGCGG 1 cut(s) 1123
CseI GACGC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 167, 197
CviAII CATG 7 cut(s) 289, 686, 916, 1154, 1259, 1361, 1411
CviQI GTAC 2 cut(s) 167, 197
DdeI CTNAG 9 cut(s) 48, 64, 91, 200, 236, 669, 990, 1035, 1311
DpnI GATC 5 cut(s) 46, 213, 341, 620, 1407
DpnII GATC 5 cut(s) 44, 211, 339, 618, 1405
DrdI GACNNNNNNGTC 1 cut(s) 1137
DseDI GACNNNNNNGTC 1 cut(s) 1137
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 3 cut(s) 854, 867, 1080
EarI CTCTTC 3 cut(s) 854, 867, 1080
EciI GGCGGA 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 1283
Eco47I GGWCC 1 cut(s) 1117
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 1294
FaeI CATG 7 cut(s) 292, 689, 919, 1157, 1262, 1364, 1414
FatI CATG 7 cut(s) 288, 685, 915, 1153, 1258, 1360, 1410
FauI CCCGC 2 cut(s) 631, 1115
FauNDI CATATG 1 cut(s) 248
FbaI TGATCA 2 cut(s) 339, 618
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 96, 1308
FokI GGATG 3 cut(s) 138, 986, 1241
Fsp4HI GCNGC 2 cut(s) 96, 1308
FspBI CTAG 2 cut(s) 717, 1016
GluI GCNGC 2 cut(s) 96, 1308
HaeIII GGCC 1 cut(s) 114
HgaI GACGC 1 cut(s) 662
Hin1II CATG 7 cut(s) 292, 689, 919, 1157, 1262, 1364, 1414
HindIII AAGCTT 2 cut(s) 300, 1313
HphI GGTGA 5 cut(s) 523, 758, 850, 1021, 1409
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 6 cut(s) 366, 411, 816, 1036, 1110, 1252
Hpy188III TCNNGA 9 cut(s) 140, 142, 470, 593, 725, 1190, 1211, 1219, 1411
Hpy8I GTNNAC 1 cut(s) 294
Hpy99I CGWCG 1 cut(s) 616
HpyAV CCTTC 5 cut(s) 45, 722, 973, 1211, 1219
HpyCH4III ACNGT 6 cut(s) 88, 281, 496, 704, 797, 1057
HpyCH4IV ACGT 4 cut(s) 73, 348, 611, 710
HpyCH4V TGCA 6 cut(s) 129, 488, 633, 738, 915, 1149
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 443, 1316
HpyF3I CTNAG 9 cut(s) 48, 64, 91, 200, 236, 669, 990, 1035, 1311
HpySE526I ACGT 4 cut(s) 73, 348, 611, 710
Hsp92II CATG 7 cut(s) 292, 689, 919, 1157, 1262, 1364, 1414
Ksp22I TGATCA 2 cut(s) 339, 618
KspI CCGCGG 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 44, 211, 339, 618, 1405
Lsp1109I GCAGC 1 cut(s) 1294
LweI GCATC 2 cut(s) 116, 197
MaeI CTAG 2 cut(s) 717, 1016
MaeII ACGT 4 cut(s) 73, 348, 611, 710
MaeIII GTNAC 3 cut(s) 706, 823, 928
MalI GATC 5 cut(s) 46, 213, 341, 620, 1407
MboI GATC 5 cut(s) 44, 211, 339, 618, 1405
MboII GAAGA 9 cut(s) 216, 383, 803, 871, 884, 1097, 1234, 1399, 1415
MflI RGATCY 1 cut(s) 1405
MluCI AATT 7 cut(s) 7, 149, 170, 221, 473, 505, 1294
MlyI GAGTC 4 cut(s) 170, 406, 1241, 1423
MmeI TCCRAC 1 cut(s) 1158
MseI TTAA 8 cut(s) 80, 315, 384, 390, 458, 477, 699, 1432
MspA1I CMGCKG 1 cut(s) 1122
MspCI CTTAAG 1 cut(s) 314
Mva1269I GAATGC 2 cut(s) 380, 823
MvnI CGCG 1 cut(s) 1122
MwoI GCNNNNNNNGC 3 cut(s) 434, 443, 1316
NdeI CATATG 1 cut(s) 248
NdeII GATC 5 cut(s) 44, 211, 339, 618, 1405
NlaIII CATG 7 cut(s) 292, 689, 919, 1157, 1262, 1364, 1414
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 706
NspI RCATGY 3 cut(s) 292, 919, 1262
PaeI GCATGC 1 cut(s) 919
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 1410
PciI ACATGT 1 cut(s) 288
PctI GAATGC 2 cut(s) 380, 823
PfeI GAWTC 6 cut(s) 356, 430, 721, 841, 922, 946
PkrI GCNGC 2 cut(s) 97, 1309
PleI GAGTC 4 cut(s) 170, 406, 1241, 1422
PpsI GAGTC 4 cut(s) 170, 406, 1241, 1422
PscI ACATGT 1 cut(s) 288
PspPI GGNCC 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 1405
RsaI GTAC 2 cut(s) 168, 198
RsaNI GTAC 2 cut(s) 167, 197
SacII CCGCGG 1 cut(s) 1123
SaqAI TTAA 8 cut(s) 80, 315, 384, 390, 458, 477, 699, 1432
SatI GCNGC 2 cut(s) 96, 1308
Sau3AI GATC 5 cut(s) 44, 211, 339, 618, 1405
Sau96I GGNCC 1 cut(s) 1117
SchI GAGTC 4 cut(s) 170, 406, 1241, 1423
SfaNI GCATC 2 cut(s) 116, 197
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 1123
SgrBI CCGCGG 1 cut(s) 1123
SinI GGWCC 1 cut(s) 1117
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 140, 314
SmoI CTYRAG 2 cut(s) 140, 314
SphI GCATGC 1 cut(s) 919
Sse9I AATT 7 cut(s) 7, 149, 170, 221, 473, 505, 1294
SsiI CCGC 8 cut(s) 96, 638, 776, 821, 1020, 1120, 1122, 1262
SspMI CTAG 2 cut(s) 717, 1016
TaaI ACNGT 6 cut(s) 88, 281, 496, 704, 797, 1057
TaiI ACGT 4 cut(s) 76, 351, 614, 713
TaqI TCGA 4 cut(s) 141, 614, 1191, 1218
TaqII GACCGA 1 cut(s) 1265
TasI AATT 7 cut(s) 7, 149, 170, 221, 473, 505, 1294
TatI WGTACW 1 cut(s) 166
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 6 cut(s) 356, 430, 721, 841, 922, 946
Tru1I TTAA 8 cut(s) 80, 315, 384, 390, 458, 477, 699, 1432
Tru9I TTAA 8 cut(s) 80, 315, 384, 390, 458, 477, 699, 1432
TscAI CASTG 3 cut(s) 286, 499, 709
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 1 cut(s) 1307
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 4 cut(s) 27, 235, 1088, 1416
TspGWI ACGGA 2 cut(s) 1058, 1094
TspRI CASTG 3 cut(s) 286, 499, 709
Vha464I CTTAAG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 1117
XapI RAATTY 3 cut(s) 7, 473, 1294
XceI RCATGY 3 cut(s) 292, 919, 1262
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 2 cut(s) 717, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.