pycom04g05510

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008335
Physical Location & Seq
Forward (+)
5315748 .. 5317178
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05510.1

Sequence Viewer

Length: 1431 bp
ATGATGAACCTTTCTTCAATACATCGGTTCTCCTCCTCCTCAGTCGGCCTGAAAAATCACACATTATCAATCCTGAACTTCTCTACGGCGGCGGCGGCCGAAACAGTGTACAATCACCTGCAGAAAAATGGTGGCAACGTAGAGGAAATCCTTGCCTCGGTTCAGGTGAAATTGGACTCCAAATATGTAAGTCAGGTTTTACAGAGATGCTATGGTAGCCGTTCCCAAATGGGTCTTAGGTTTTTCATATGGGCAGGTCTTCAATCCACTTATAGGCACAGCTATTTTATGTATAGCAAAGCTTGTGAATTGTGTGAGATTAGGATAAAACCGACTGTGATTTTCGATGTTTTGGAGGCTTATAGAGTTGAAGGGTGCTCTGTTAATCTGAAGACGTTTAAGGTTGTTTTTAATTTGTGTAAAGAAGCTAAGCTTGCTGATGAAGCTTTAAGGGTATTAAGGAAAATGCCGGAATTTGGTTTGCATCCGGATACTACGGTGTATAATGTCGTTATAAGATTGTTTTGTGATAATGGTGATATGGATGTGGCTGAAAGTTTGGTGAAGGAGATGCGGTTGGTGGATCTTTATCCCGATTTGATCACGTACATGGTACTGATCAAAGGGTTTTGTAATGTGGGTCGGTTAGATAATGCTTGTGGCTTGTTTAAGGTTATGAAGGAACATGGTTGTTTGCCGAATGCTGTGGTGTACTCGGCACTTCTTGATGGATTTTGTAGGTGTGATAATATGGAGAGAGCCTTGGAGTTATTGAAGGAAATGGAGAAGGAAGGTGGGGATTGTAGTCCTAATGTAGTAACATATACATCTGTGATTCAAAAGCTTTGTGACAAGGGACGGTCCACTAATGCCTTGGTTATTTTGGACCGAATGGAAGCTTGTGGATGTGCCCCAAGTCGCGTTACAGTTAGTTCTCTGATTAAGGGTTTGTGTGTCGAGGATCGAGTTGAAGAGGCTTGTAAGTTGATTGGTAGAGTTGTTGCTGGACGTAGTGTCACATATAGTGATTGCTATAGCTCTCTTGTTGTGTCATTGGCAAGAGGTGGGAAAAGTGAGGAGACAGAGAAGGTCTTTAGGATGATGGTTGATGGCAGGGTCAAACCAAATAGTTTGGCCTCTAGCATTATTTTGAAAAAACTTTGTTTGGAGGGTCGGGTGGTAGAAGCGTTGTGCTTGTTTGATGAACTTGAGAAAATGCAATGCTTGTCTTCCATCGACTCTGATGCGTATTCTATCCTCTTAGTGGGGCTTTGTCAACAAAAGCATTTAGTGGAGGCTGCAAAGCTGGCAAGAGCAATGCTCAATAAAGGAATTAAACTGAAAGCTCCTTATGTTGACACAATAACTGAGATATTGAAGAAATCCGGAGATGAGGAGTTAATTAAGAATTTGACGAGAATTGCAAGCTGA

Protein Analysis

477

Amino Acids

52.96

Weight (kDa)

8.24

Isoelectric Point (pI)

32.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 116 - 242 4.4e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 160 - 192 8.1e-08 PPR repeat
PPR_2 PF13041 163 - 212 1.4e-12 PPR repeat family
PPR_3 PF13812 186 - 243 1.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 196 - 227 1.2e-08 PPR repeat
PPR_2 PF13041 198 - 247 3.6e-15 PPR repeat family
PPR PF01535 201 - 231 1.6e-06 PPR repeat
PPR_long PF17177 217 - 315 6.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 230 - 262 6e-11 PPR repeat
PPR_2 PF13041 233 - 284 2.9e-14 PPR repeat family
PPR_3 PF13812 234 - 280 3.6e-06 Pentatricopeptide repeat domain
PPR PF01535 236 - 265 7.3e-08 PPR repeat
PPR_3 PF13812 258 - 315 5.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 299 6.3e-08 PPR repeat
PPR_2 PF13041 270 - 318 1.7e-12 PPR repeat family
PPR_1 PF12854 302 - 329 9.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AarI CACCTGC 1 cut(s) 126
Acc36I ACCTGC 2 cut(s) 126, 245
AccII CGCG 1 cut(s) 921
AccIII TCCGGA 2 cut(s) 487, 1385
AciI CCGC 4 cut(s) 89, 92, 95, 574
AclWI GGATC 2 cut(s) 591, 969
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 473, 1408
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 4 cut(s) 110, 608, 615, 713
AfiI CCNNNNNNNGG 4 cut(s) 157, 273, 476, 1264
AgsI TTSAA 8 cut(s) 18, 263, 371, 775, 839, 971, 1153, 1378
AhdI GACNNNNNGTC 1 cut(s) 1013
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 1073
AlwI GGATC 2 cut(s) 591, 969
Aor13HI TCCGGA 2 cut(s) 487, 1385
AoxI GGCC 3 cut(s) 46, 96, 1134
ApeKI GCWGC 1 cut(s) 1298
ApoI RAATTY 2 cut(s) 473, 1408
AspS9I GGNCC 2 cut(s) 861, 886
AsuHPI GGTGA 4 cut(s) 107, 178, 548, 574
AvaII GGWCC 2 cut(s) 861, 886
BaeGI GKGCMC 1 cut(s) 913
BaeI ACNNNNGTAYC 2 cut(s) 483, 516
BbsI GAAGAC 3 cut(s) 251, 398, 1221
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 1285
BccI CCATC 4 cut(s) 722, 1096, 1103, 1241
BceAI ACGGC 2 cut(s) 102, 204
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciVI GTATCC 1 cut(s) 484
BclI TGATCA 2 cut(s) 600, 618
BcoDI GTCTC 1 cut(s) 1073
BfaI CTAG 1 cut(s) 1140
BfmI CTRYAG 2 cut(s) 119, 1033
BfuAI ACCTGC 2 cut(s) 126, 245
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 4 cut(s) 90, 93, 96, 1299
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 4 cut(s) 91, 94, 97, 1300
Bme18I GGWCC 2 cut(s) 861, 886
BmeRI GACNNNNNGTC 1 cut(s) 1013
BmgT120I GGNCC 2 cut(s) 861, 886
BmsI GCATC 4 cut(s) 197, 493, 561, 1234
BpiI GAAGAC 3 cut(s) 251, 398, 1221
BplI GAGNNNNNCTC 2 cut(s) 1305, 1337
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 3 cut(s) 156, 762, 873
BsaWI WCCGGW 2 cut(s) 487, 1385
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 273, 476, 1264
Bse3DI GCAATG 2 cut(s) 1226, 1323
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 2 cut(s) 487, 1385
BseDI CCNNGG 3 cut(s) 156, 762, 873
BseGI GGATG 4 cut(s) 484, 550, 911, 1104
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 4 cut(s) 157, 273, 476, 1264
BseMI GCAATG 2 cut(s) 1226, 1323
BseMII CTCAG 2 cut(s) 54, 1359
BseRI GAGGAG 5 cut(s) 22, 25, 28, 1091, 1409
BseSI GKGCMC 1 cut(s) 913
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 1285
Bsh1236I CGCG 1 cut(s) 921
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 48, 98, 1136
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 3 cut(s) 470, 488, 1386
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 4 cut(s) 157, 273, 476, 1264
BsmAI GTCTC 1 cut(s) 1073
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 3 cut(s) 48, 98, 1136
Bsp1286I GDGCHC 2 cut(s) 380, 913
Bsp13I TCCGGA 2 cut(s) 487, 1385
Bsp1407I TGTACA 1 cut(s) 108
Bsp143I GATC 4 cut(s) 583, 600, 618, 961
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 4 cut(s) 89, 92, 95, 574
BspANI GGCC 3 cut(s) 48, 98, 1136
BspCNI CTCAG 2 cut(s) 53, 1360
BspEI TCCGGA 2 cut(s) 487, 1385
BspFNI CGCG 1 cut(s) 921
BspMAI CTGCAG 1 cut(s) 123
BspMI ACCTGC 2 cut(s) 126, 245
BspPI GGATC 2 cut(s) 591, 969
BsrDI GCAATG 2 cut(s) 1226, 1323
BsrGI TGTACA 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 156, 762, 873
BssMI GATC 4 cut(s) 583, 600, 618, 961
BssT1I CCWWGG 2 cut(s) 762, 873
Bst4CI ACNGT 5 cut(s) 106, 337, 499, 861, 928
Bst6I CTCTTC 1 cut(s) 966
BstAUI TGTACA 1 cut(s) 108
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 435, 1308, 1426
BstDEI CTNAG 5 cut(s) 40, 236, 429, 1261, 1368
BstF5I GGATG 4 cut(s) 484, 550, 911, 1104
BstFNI CGCG 1 cut(s) 921
BstKTI GATC 4 cut(s) 586, 603, 621, 964
BstMAI GTCTC 1 cut(s) 1073
BstMBI GATC 4 cut(s) 583, 600, 618, 961
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 5 cut(s) 95, 216, 434, 443, 1307
BstSFI CTRYAG 2 cut(s) 119, 1033
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 1 cut(s) 1285
BstV2I GAAGAC 3 cut(s) 251, 398, 1221
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 96
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 3 cut(s) 48, 98, 1136
BtsCI GGATG 4 cut(s) 484, 550, 911, 1104
BtsIMutI CAGTG 1 cut(s) 111
BveI ACCTGC 2 cut(s) 126, 245
Cac8I GCNNGC 3 cut(s) 435, 1308, 1426
Cfr13I GGNCC 2 cut(s) 861, 886
Csp6I GTAC 4 cut(s) 109, 607, 614, 712
CviAII CATG 2 cut(s) 610, 686
CviQI GTAC 4 cut(s) 109, 607, 614, 712
DdeI CTNAG 5 cut(s) 40, 236, 429, 1261, 1368
DpnI GATC 4 cut(s) 585, 602, 620, 963
DpnII GATC 4 cut(s) 583, 600, 618, 961
DriI GACNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 966
Eam1105I GACNNNNNGTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 966
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 762, 873
Eco47I GGWCC 2 cut(s) 861, 886
Eco52I CGGCCG 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 410
EcoT14I CCWWGG 2 cut(s) 762, 873
ErhI CCWWGG 2 cut(s) 762, 873
FaeI CATG 2 cut(s) 613, 689
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 870
FatI CATG 2 cut(s) 609, 685
FauNDI CATATG 1 cut(s) 248
FbaI TGATCA 2 cut(s) 600, 618
Fnu4HI GCNGC 4 cut(s) 90, 93, 96, 1299
FokI GGATG 4 cut(s) 471, 557, 918, 1111
Fsp4HI GCNGC 4 cut(s) 90, 93, 96, 1299
FspBI CTAG 1 cut(s) 1140
GluI GCNGC 4 cut(s) 90, 93, 96, 1299
HaeIII GGCC 3 cut(s) 48, 98, 1136
HapII CCGG 3 cut(s) 470, 488, 1386
Hin1II CATG 2 cut(s) 613, 689
HincII GTYRAC 2 cut(s) 1277, 1357
HindII GTYRAC 2 cut(s) 1277, 1357
HindIII AAGCTT 5 cut(s) 300, 431, 444, 842, 897
HinfI GANTC 3 cut(s) 176, 835, 1238
HpaII CCGG 3 cut(s) 470, 488, 1386
HphI GGTGA 4 cut(s) 107, 178, 548, 574
Hpy166II GTNNAC 5 cut(s) 109, 712, 864, 1277, 1357
Hpy188I TCNGA 3 cut(s) 390, 939, 1243
Hpy188III TCNNGA 5 cut(s) 73, 488, 593, 725, 1386
Hpy8I GTNNAC 5 cut(s) 109, 712, 864, 1277, 1357
HpyAV CCTTC 7 cut(s) 365, 559, 673, 769, 781, 785, 1081
HpyCH4III ACNGT 5 cut(s) 106, 337, 499, 861, 928
HpyCH4IV ACGT 4 cut(s) 138, 395, 605, 1009
HpyCH4V TGCA 5 cut(s) 121, 484, 1219, 1301, 1424
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 216, 434, 443, 1307
HpyF3I CTNAG 5 cut(s) 40, 236, 429, 1261, 1368
HpySE526I ACGT 4 cut(s) 138, 395, 605, 1009
Hsp92II CATG 2 cut(s) 613, 689
Kpn2I TCCGGA 2 cut(s) 487, 1385
Ksp22I TGATCA 2 cut(s) 600, 618
Kzo9I GATC 4 cut(s) 583, 600, 618, 961
LmnI GCTCC 1 cut(s) 1351
Lsp1109I GCAGC 1 cut(s) 1285
LweI GCATC 4 cut(s) 197, 493, 561, 1234
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 4 cut(s) 138, 395, 605, 1009
MaeIII GTNAC 4 cut(s) 817, 848, 922, 1015
MalI GATC 4 cut(s) 585, 602, 620, 963
MboI GATC 4 cut(s) 583, 600, 618, 961
MboII GAAGA 6 cut(s) 6, 251, 403, 983, 1221, 1390
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 380, 913
MluCI AATT 8 cut(s) 170, 308, 412, 473, 1332, 1401, 1408, 1419
MlyI GAGTC 2 cut(s) 170, 1232
MroI TCCGGA 2 cut(s) 487, 1385
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 3 cut(s) 470, 488, 1386
Mva1269I GAATGC 1 cut(s) 706
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 5 cut(s) 95, 216, 434, 443, 1307
NdeI CATATG 1 cut(s) 248
NdeII GATC 4 cut(s) 583, 600, 618, 961
NlaIII CATG 2 cut(s) 613, 689
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 2 cut(s) 848, 1015
PacI TTAATTAA 1 cut(s) 1404
PaqCI CACCTGC 1 cut(s) 126
PctI GAATGC 1 cut(s) 706
PfeI GAWTC 1 cut(s) 835
PkrI GCNGC 4 cut(s) 91, 94, 97, 1300
PleI GAGTC 2 cut(s) 170, 1232
PpsI GAGTC 2 cut(s) 170, 1232
Ppu21I YACGTR 1 cut(s) 606
PsiI TTATAA 1 cut(s) 515
PspPI GGNCC 2 cut(s) 861, 886
PstI CTGCAG 1 cut(s) 123
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 4 cut(s) 110, 608, 615, 713
RsaNI GTAC 4 cut(s) 109, 607, 614, 712
RseI CAYNNNNRTG 1 cut(s) 608
SatI GCNGC 4 cut(s) 90, 93, 96, 1299
Sau3AI GATC 4 cut(s) 583, 600, 618, 961
Sau96I GGNCC 2 cut(s) 861, 886
SchI GAGTC 2 cut(s) 170, 1232
SduI GDGCHC 2 cut(s) 380, 913
SfaNI GCATC 4 cut(s) 197, 493, 561, 1234
SfcI CTRYAG 2 cut(s) 119, 1033
SinI GGWCC 2 cut(s) 861, 886
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
Sse9I AATT 8 cut(s) 170, 308, 412, 473, 1332, 1401, 1408, 1419
SsiI CCGC 4 cut(s) 89, 92, 95, 574
SspMI CTAG 1 cut(s) 1140
StyI CCWWGG 2 cut(s) 762, 873
TaaI ACNGT 5 cut(s) 106, 337, 499, 861, 928
TaiI ACGT 4 cut(s) 141, 398, 608, 1012
TaqI TCGA 4 cut(s) 345, 957, 964, 1236
TaqII GACCGA 1 cut(s) 903
TasI AATT 8 cut(s) 170, 308, 412, 473, 1332, 1401, 1408, 1419
TatI WGTACW 2 cut(s) 108, 711
TauI GCSGC 3 cut(s) 92, 95, 98
TfiI GAWTC 1 cut(s) 835
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 2 cut(s) 848, 1015
TseI GCWGC 1 cut(s) 1298
Tsp45I GTSAC 2 cut(s) 848, 1015
TspDTI ATGAA 5 cut(s) 20, 235, 456, 692, 1218
TspRI CASTG 1 cut(s) 111
VpaK11BI GGWCC 2 cut(s) 861, 886
XapI RAATTY 2 cut(s) 473, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XspI CTAG 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.