MD04G1061400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008335
Physical Location & Seq
Forward (+)
8091715 .. 8094707
2993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1061400.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGATGAACCTTTCTTCAATACATCGGTTCTTCTCCTCCTCAGTCGGCCTGAAAAATCACAAATTATCAATCCTGAACTTCTCTACGGCGTCGGCGGCGGCCGAAACAGTGTACAATCACCTGCAGAAAAATGGTAGCAACGTAGAGGAAATCCTTGCCTCGGTTCAGGTGAAATTGGACTCCAAATATGTAAGTCAGGTTTTACAGAGATGCTATGGTAGCCGTTCCCAAATGGGTCTTAGGTTTTTCATATGGGCAGGTCTTCAATCCACTTATAGGCACAGCTATTTTATGTATAGCAAAGCTTGTGAATTGTGTGAGATTAGGATAAAACCGAGTGTGATTTTCGATGTTTTGGAGGCTTATAGAATTGAAGGGTGCTCTGTTAATCTGAAGACGTTTAAGGTTGTTTTTAATTTGTGTAAAGAAGCTAAGCTTGCTGATGAAGCATTAAGGGTATTAAGGAAAATGCCGGAATTTGGTTTGCGTCCGGATACTACGGTGTATAATGTCGTTATAAGATTGTTTTGTGATAATGGTGATATGGATATGGCTGAAAGTTTGGTGAAGGAGATGCGGTTGGTGGATCTTTATCCCGATTTGATCACGTACATGGTACTGATCAAGGGGTTTTGTAATGTGGGTCGGTTAGATAATGCTTGTGGCTTGTTTAAGGTTATGAAGGAACATGGTTGTTTGCCGAATGCTGTGGTGTACTCGGCACTTCTTGATGGATTTTGTAGGTGTGATAATATGGAGAGAGCTTTGGAGTTATTGAAGGAAATGGAGAAGGAAGGTGGGGATTGTAGTCCTAATGTAGTAACATATACATCTGTGATTCAAAAGCTTTGTGACAAGGGACGGTCCACGAATGCCTTGGTTATTTTGGACCGAATGGAAGCTTGTGGATGTGCCCCAAGTCGCGTTACAGTTAGTTCTCTGATTAAGGGTTTGTGCGTTGAGGATCGAGTTGAAGAGGCTTGTAAGTTGATTGATAGAGTTGTTGCTGGACGTAGTGTCACATATAGTGATTGCTATAGCTCTCTTGTTGTGTCGTTGGCAAGAGGTGGGAAAAGTGAGGAGGCAGAGAAGGTCTTTAGGATGATGGTTGATGGCAGGGTCAAACCAAATAGTTTGGCCTCTAGCATTATTTTGAAAAAACTTTGTTTGGAGGGTCAGGTGGTAGAAGCGTTGTGCTTGTTTGATGAACTTGAGAAAATGCAATGCTTGTCTTCCATCGACTCTGATGTGTATTCTATCCTCTTAGTGGGGCTTTGTCAACAAAAGCATTTAGTGGAGGCTGCAAAGCTGGCAAGAGCAATGCTCAATAAAGGAATTAAACTGAAAGCTCCTTATGTTGACACAATAGCTGAGATATTGAAGAAATCCGGAGATGAGGAGTTAATTAAGAATTTGACTAGAATTGCAAGGTGA

Protein Analysis

478

Amino Acids

53.28

Weight (kDa)

8.34

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 118 - 243 3.6e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 161 - 193 5.9e-07 PPR repeat
PPR_2 PF13041 164 - 213 4.6e-13 PPR repeat family
PPR PF01535 168 - 193 8.2e-06 PPR repeat
PPR_3 PF13812 187 - 244 1.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 197 - 228 1.2e-08 PPR repeat
PPR_2 PF13041 199 - 248 3.6e-15 PPR repeat family
PPR PF01535 202 - 232 1.6e-06 PPR repeat
PPR_long PF17177 220 - 316 8.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 231 - 263 6e-11 PPR repeat
PPR_2 PF13041 234 - 285 2.8e-14 PPR repeat family
PPR_3 PF13812 235 - 281 3.7e-06 Pentatricopeptide repeat domain
PPR PF01535 237 - 266 7.3e-08 PPR repeat
PPR_3 PF13812 259 - 316 5.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 268 - 300 6.3e-08 PPR repeat
PPR_2 PF13041 271 - 319 1.7e-12 PPR repeat family
PPR_1 PF12854 303 - 333 2.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 2 cut(s) 129, 248
AccII CGCG 1 cut(s) 924
AccIII TCCGGA 2 cut(s) 490, 1388
AciI CCGC 3 cut(s) 95, 98, 577
AclWI GGATC 2 cut(s) 594, 972
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 476, 1411
AcuI CTGAAG 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 4 cut(s) 113, 611, 618, 716
AfiI CCNNNNNNNGG 4 cut(s) 160, 276, 479, 1267
AgsI TTSAA 8 cut(s) 18, 266, 374, 778, 842, 974, 1156, 1381
AhdI GACNNNNNGTC 1 cut(s) 1016
Alw21I GWGCWC 1 cut(s) 383
AlwI GGATC 2 cut(s) 594, 972
Aor13HI TCCGGA 2 cut(s) 490, 1388
AoxI GGCC 3 cut(s) 46, 99, 1137
ApeKI GCWGC 1 cut(s) 1301
ApoI RAATTY 2 cut(s) 476, 1411
AspS9I GGNCC 2 cut(s) 864, 889
AsuHPI GGTGA 4 cut(s) 110, 181, 551, 577
AvaII GGWCC 2 cut(s) 864, 889
BaeGI GKGCMC 1 cut(s) 916
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BbsI GAAGAC 3 cut(s) 254, 401, 1224
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 1288
BccI CCATC 4 cut(s) 725, 1099, 1106, 1244
BceAI ACGGC 2 cut(s) 102, 207
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 2 cut(s) 603, 621
BfaI CTAG 2 cut(s) 1143, 1419
BfmI CTRYAG 2 cut(s) 122, 1036
BfuAI ACCTGC 2 cut(s) 129, 248
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 3 cut(s) 96, 99, 1302
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 3 cut(s) 97, 100, 1303
Bme18I GGWCC 2 cut(s) 864, 889
BmeRI GACNNNNNGTC 1 cut(s) 1016
BmgT120I GGNCC 2 cut(s) 864, 889
BmsI GCATC 2 cut(s) 200, 564
BpiI GAAGAC 3 cut(s) 254, 401, 1224
BplI GAGNNNNNCTC 2 cut(s) 1308, 1340
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 1232
BsaAI YACGTR 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 159, 876
BsaWI WCCGGW 2 cut(s) 490, 1388
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 276, 479, 1267
Bse3DI GCAATG 2 cut(s) 1229, 1326
Bse8I GATNNNNATC 1 cut(s) 591
BseAI TCCGGA 2 cut(s) 490, 1388
BseDI CCNNGG 2 cut(s) 159, 876
BseGI GGATG 2 cut(s) 914, 1107
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 4 cut(s) 160, 276, 479, 1267
BseMI GCAATG 2 cut(s) 1229, 1326
BseMII CTCAG 2 cut(s) 54, 1362
BseRI GAGGAG 4 cut(s) 25, 28, 1094, 1412
BseSI GKGCMC 1 cut(s) 916
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 1288
Bsh1236I CGCG 1 cut(s) 924
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 48, 101, 1139
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 3 cut(s) 473, 491, 1389
BslFI GGGAC 1 cut(s) 873
BslI CCNNNNNNNGG 4 cut(s) 160, 276, 479, 1267
BsmFI GGGAC 1 cut(s) 873
BsmI GAATGC 2 cut(s) 709, 877
BsnI GGCC 3 cut(s) 48, 101, 1139
Bsp1286I GDGCHC 2 cut(s) 383, 916
Bsp13I TCCGGA 2 cut(s) 490, 1388
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 4 cut(s) 586, 603, 621, 964
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 3 cut(s) 95, 98, 577
BspANI GGCC 3 cut(s) 48, 101, 1139
BspCNI CTCAG 2 cut(s) 53, 1363
BspEI TCCGGA 2 cut(s) 490, 1388
BspFNI CGCG 1 cut(s) 924
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 2 cut(s) 129, 248
BspPI GGATC 2 cut(s) 594, 972
BsrDI GCAATG 2 cut(s) 1229, 1326
BsrGI TGTACA 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 159, 876
BssMI GATC 4 cut(s) 586, 603, 621, 964
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 876
Bst4CI ACNGT 4 cut(s) 109, 502, 864, 931
Bst6I CTCTTC 1 cut(s) 969
BstACI GRCGYC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 438, 1311
BstDEI CTNAG 5 cut(s) 40, 239, 432, 1264, 1371
BstF5I GGATG 2 cut(s) 914, 1107
BstFNI CGCG 1 cut(s) 924
BstKTI GATC 4 cut(s) 589, 606, 624, 967
BstMBI GATC 4 cut(s) 586, 603, 621, 964
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 5 cut(s) 95, 219, 437, 446, 1310
BstSFI CTRYAG 2 cut(s) 122, 1036
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 1 cut(s) 1288
BstV2I GAAGAC 3 cut(s) 254, 401, 1224
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 3 cut(s) 48, 101, 1139
BtsCI GGATG 2 cut(s) 914, 1107
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 2 cut(s) 129, 248
Cac8I GCNNGC 2 cut(s) 438, 1311
Cfr13I GGNCC 2 cut(s) 864, 889
CseI GACGC 2 cut(s) 78, 476
Csp6I GTAC 4 cut(s) 112, 610, 617, 715
CviAII CATG 2 cut(s) 613, 689
CviQI GTAC 4 cut(s) 112, 610, 617, 715
DdeI CTNAG 5 cut(s) 40, 239, 432, 1264, 1371
DpnI GATC 4 cut(s) 588, 605, 623, 966
DpnII GATC 4 cut(s) 586, 603, 621, 964
DriI GACNNNNNGTC 1 cut(s) 1016
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 969
Eam1105I GACNNNNNGTC 1 cut(s) 1016
EarI CTCTTC 1 cut(s) 969
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 876
Eco47I GGWCC 2 cut(s) 864, 889
Eco52I CGGCCG 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 2 cut(s) 616, 692
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 873
FatI CATG 2 cut(s) 612, 688
FauNDI CATATG 1 cut(s) 251
FbaI TGATCA 2 cut(s) 603, 621
Fnu4HI GCNGC 3 cut(s) 96, 99, 1302
FokI GGATG 2 cut(s) 921, 1114
Fsp4HI GCNGC 3 cut(s) 96, 99, 1302
FspBI CTAG 2 cut(s) 1143, 1419
GluI GCNGC 3 cut(s) 96, 99, 1302
HaeIII GGCC 3 cut(s) 48, 101, 1139
HapII CCGG 3 cut(s) 473, 491, 1389
HgaI GACGC 2 cut(s) 78, 476
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 2 cut(s) 616, 692
HincII GTYRAC 2 cut(s) 1280, 1360
HindII GTYRAC 2 cut(s) 1280, 1360
HindIII AAGCTT 4 cut(s) 303, 434, 845, 900
HinfI GANTC 3 cut(s) 179, 838, 1241
HpaII CCGG 3 cut(s) 473, 491, 1389
HphI GGTGA 4 cut(s) 110, 181, 551, 577
Hpy166II GTNNAC 5 cut(s) 112, 715, 867, 1280, 1360
Hpy188I TCNGA 3 cut(s) 393, 942, 1246
Hpy188III TCNNGA 5 cut(s) 73, 491, 596, 728, 1389
Hpy8I GTNNAC 5 cut(s) 112, 715, 867, 1280, 1360
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 7 cut(s) 368, 562, 676, 772, 784, 788, 1084
HpyCH4III ACNGT 4 cut(s) 109, 502, 864, 931
HpyCH4IV ACGT 4 cut(s) 141, 398, 608, 1012
HpyCH4V TGCA 4 cut(s) 124, 1222, 1304, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 219, 437, 446, 1310
HpyF3I CTNAG 5 cut(s) 40, 239, 432, 1264, 1371
HpySE526I ACGT 4 cut(s) 141, 398, 608, 1012
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 2 cut(s) 616, 692
Kpn2I TCCGGA 2 cut(s) 490, 1388
Ksp22I TGATCA 2 cut(s) 603, 621
Kzo9I GATC 4 cut(s) 586, 603, 621, 964
LmnI GCTCC 1 cut(s) 1354
Lsp1109I GCAGC 1 cut(s) 1288
LweI GCATC 2 cut(s) 200, 564
MaeI CTAG 2 cut(s) 1143, 1419
MaeII ACGT 4 cut(s) 141, 398, 608, 1012
MaeIII GTNAC 4 cut(s) 820, 851, 925, 1018
MalI GATC 4 cut(s) 588, 605, 623, 966
MboI GATC 4 cut(s) 586, 603, 621, 964
MboII GAAGA 7 cut(s) 6, 22, 254, 406, 986, 1224, 1393
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 2 cut(s) 383, 916
MlyI GAGTC 2 cut(s) 173, 1235
MroI TCCGGA 2 cut(s) 490, 1388
MslI CAYNNNNRTG 1 cut(s) 611
MspI CCGG 3 cut(s) 473, 491, 1389
Mva1269I GAATGC 2 cut(s) 709, 877
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 5 cut(s) 95, 219, 437, 446, 1310
NdeI CATATG 1 cut(s) 251
NdeII GATC 4 cut(s) 586, 603, 621, 964
NlaIII CATG 2 cut(s) 616, 692
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 2 cut(s) 851, 1018
PacI TTAATTAA 1 cut(s) 1407
PaqCI CACCTGC 1 cut(s) 129
PctI GAATGC 2 cut(s) 709, 877
PfeI GAWTC 1 cut(s) 838
PkrI GCNGC 3 cut(s) 97, 100, 1303
PleI GAGTC 2 cut(s) 173, 1235
PpsI GAGTC 2 cut(s) 173, 1235
Ppu21I YACGTR 1 cut(s) 609
PsiI TTATAA 1 cut(s) 518
PspPI GGNCC 2 cut(s) 864, 889
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 4 cut(s) 113, 611, 618, 716
RsaNI GTAC 4 cut(s) 112, 610, 617, 715
RseI CAYNNNNRTG 1 cut(s) 611
SatI GCNGC 3 cut(s) 96, 99, 1302
Sau3AI GATC 4 cut(s) 586, 603, 621, 964
Sau96I GGNCC 2 cut(s) 864, 889
SchI GAGTC 2 cut(s) 173, 1235
SduI GDGCHC 2 cut(s) 383, 916
SfaNI GCATC 2 cut(s) 200, 564
SfcI CTRYAG 2 cut(s) 122, 1036
SinI GGWCC 2 cut(s) 864, 889
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 1211
SmoI CTYRAG 1 cut(s) 1211
SsiI CCGC 3 cut(s) 95, 98, 577
SspMI CTAG 2 cut(s) 1143, 1419
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 4 cut(s) 109, 502, 864, 931
TaiI ACGT 4 cut(s) 144, 401, 611, 1015
TaqI TCGA 3 cut(s) 348, 967, 1239
TaqII GACCGA 1 cut(s) 906
TatI WGTACW 2 cut(s) 111, 714
TauI GCSGC 2 cut(s) 98, 101
TfiI GAWTC 1 cut(s) 838
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 2 cut(s) 851, 1018
TseI GCWGC 1 cut(s) 1301
Tsp45I GTSAC 2 cut(s) 851, 1018
TspDTI ATGAA 5 cut(s) 20, 238, 459, 695, 1221
TspRI CASTG 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 864, 889
XapI RAATTY 2 cut(s) 476, 1411
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XspI CTAG 2 cut(s) 1143, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.