FvH4_5g19630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008335
Physical Location & Seq
Forward (+)
11489643 .. 11493880
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19630.t2

Sequence Viewer

Length: 1425 bp
ATGACGACCCTTTCTTCAATCTCTCGATTCCTCTCTTCCTCAATTCGCCTCCAAAATCACAACCTCTCAATCTCTCGCTTCTCTACTGCCACGTCAGCAGAGACGGTCTTGAATCACCTCCACAGAAATGGCGGCAAAATTGAGAAAACCCTAGACTCAATGAGGCTCAATTTGGACGCCAAATGTGTAAGTCAGGTCTTACAGAGATGCTATCCCACCCAATCCCAATTGGGTCTTAGATTTTTCATATGGGCTGGGGTTCACTCCAGTTATAGGCATAGCTACTTTATGTTTAGTAAAGCTTGTGACTTGTATAAGATTAGGGAATACCCAAGTTTGATTTTTGATGTTTTGGAGGCTTATAGTGCTGAAGGTTGCTCTGTTAGTGTTAAGATGTTTAAAGTTTTGTTTAATGTGTGTAAAGAAGCTAAGCTTGCTGATGAGGCTTTGAGGGTTCTGAGGAAAATGCCGGAGTTCGGGTTGCGAGGGGATAATGTGGTGTATAATGTTGTTATCAGGCAGTTTTGTGAGAAGGGTGATATGGATATGGCTGAGAGTTTGGTGAAGGAGATGAGTGAGGTGGAGCTTTATCCGGATTTGATCACGTATATGGTGATGATTAAAGGGTTCTGTAATGTGGGTCGGTTGGATGATGCATGTGGACTGTTTATGTTTATGAAGGAAAATGGTTGTGTGCCGAATGTTGTGGTGTACTCGGCGCTTCTTGATGGATTCTGTAGGTTTGGGGATATGGAGAGAGCCTTGACGTTGTTGGAGGAGATGGAGAAGGAAGGTGGGGATTGTGGTCCTAATGTAGTAACATATACGACTGTGATTCAGTGTCTTTGTAACAAGCATCGGTCAGTGGAGGCATTGCTTGTTTTGGACCGGATGGAAGCTCGTGGTTGTTTACCAAATCGCGTCACGGTTAGTACTTTGATCACAGGTCTTGTTAAGGAGGATCAAGTTGAGCACGCTTATAAGTTGGTTGATAGAGTTGTTAAAAGTGGAAGTGTTACAAAAACTGATTGCTACAGTACTTTTGTTGTGTCTTTGGAAAGAGTTGGAAGACCTGAAGAGGCAGAGAAAGTCCTTAGGATGATGCTTAATAGTGGGGTTAAACCGAACAGTTTGGTGTGTACCATTATGTTAAAAAAATGTTGTTTGGAGGGACGTATGGTGGACGCATATTGCTTATTTGGTGAACTTGAGAAGATGGAATGCTTATCTTCTATTGAATCTGATACTTATTCTATTCTTTTGTTGGGGCTTTGTCAGCAAAGACACTTGGTGGAGGCTGCAGAGCTTGCAAGGGTAATGCTCAGTAAAGGAATTAAACTAAAAGGTCCATATGTTGATATTATATCTGAGGTCCTGGTGAAATCTGGAGACGAAGAGTTAGTTAAACAATTGACAAGGAATTGA

Protein Analysis

475

Amino Acids

53.37

Weight (kDa)

6.72

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 116 - 242 7.1e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 164 - 212 3.1e-13 PPR repeat family
PPR_3 PF13812 186 - 243 1.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 196 - 227 1.2e-07 PPR repeat
PPR_2 PF13041 198 - 247 2e-15 PPR repeat family
PPR PF01535 201 - 231 1.6e-06 PPR repeat
PPR_long PF17177 222 - 336 1.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 229 - 262 3.3e-12 PPR repeat
PPR_2 PF13041 233 - 265 9.9e-08 PPR repeat family
PPR_3 PF13812 233 - 281 5.1e-07 Pentatricopeptide repeat domain
PPR PF01535 236 - 265 1.1e-07 PPR repeat
PPR_3 PF13812 257 - 315 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 268 - 299 2.2e-07 PPR repeat
PPR_2 PF13041 270 - 319 4e-14 PPR repeat family
PPR_2 PF13041 344 - 388 1.3e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AccII CGCG 1 cut(s) 921
AccIII TCCGGA 1 cut(s) 592
AciI CCGC 1 cut(s) 132
AclWI GGATC 1 cut(s) 969
AcuI CTGAAG 2 cut(s) 390, 1095
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 1291
AfaI GTAC 4 cut(s) 713, 934, 1039, 1141
AfiI CCNNNNNNNGG 2 cut(s) 273, 476
AgsI TTSAA 3 cut(s) 18, 112, 1238
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 1 cut(s) 1374
AluBI AGCT 7 cut(s) 282, 302, 428, 433, 586, 899, 1306
AluI AGCT 7 cut(s) 282, 302, 428, 433, 586, 899, 1306
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 2 cut(s) 95, 1383
AlwI GGATC 1 cut(s) 969
Aor13HI TCCGGA 1 cut(s) 592
ApeKI GCWGC 1 cut(s) 1298
AspLEI GCGC 1 cut(s) 721
AspS9I GGNCC 4 cut(s) 806, 886, 1346, 1372
AsuHPI GGTGA 6 cut(s) 107, 548, 574, 625, 1214, 1390
AvaII GGWCC 4 cut(s) 806, 886, 1346, 1372
AxyI CCTNAGG 1 cut(s) 1094
BauI CACGAG 1 cut(s) 900
BbsI GAAGAC 1 cut(s) 1075
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 1 cut(s) 1285
BccI CCATC 4 cut(s) 722, 775, 886, 1210
BciT130I CCWGG 1 cut(s) 1376
BclI TGATCA 2 cut(s) 600, 939
BcoDI GTCTC 2 cut(s) 95, 1383
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 3 cut(s) 736, 1033, 1299
BfoI RGCGCY 1 cut(s) 722
BisI GCNGC 2 cut(s) 133, 1299
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 2 cut(s) 134, 1300
BmcAI AGTACT 2 cut(s) 934, 1039
Bme1390I CCNGG 1 cut(s) 1376
Bme18I GGWCC 4 cut(s) 806, 886, 1346, 1372
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 4 cut(s) 806, 886, 1346, 1372
BmrFI CCNGG 1 cut(s) 1376
BmsI GCATC 4 cut(s) 197, 643, 865, 1092
BpiI GAAGAC 1 cut(s) 1075
BpmI CTGGAG 2 cut(s) 250, 1407
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 177
BsaWI WCCGGW 2 cut(s) 592, 888
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 476
Bse1I ACTGG 1 cut(s) 267
Bse21I CCTNAGG 1 cut(s) 1094
Bse3DI GCAATG 1 cut(s) 872
BseAI TCCGGA 1 cut(s) 592
BseBI CCWGG 1 cut(s) 1376
BseGI GGATG 3 cut(s) 655, 897, 1104
BseLI CCNNNNNNNGG 2 cut(s) 273, 476
BseMI GCAATG 1 cut(s) 872
BseMII CTCAG 4 cut(s) 449, 543, 1336, 1359
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 1 cut(s) 1285
BseYI CCCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 921
BsiHKAI GWGCWC 1 cut(s) 975
BsiSI CCGG 3 cut(s) 470, 593, 889
BslFI GGGAC 1 cut(s) 1185
BslI CCNNNNNNNGG 2 cut(s) 273, 476
BsmAI GTCTC 2 cut(s) 95, 1383
BsmBI CGTCTC 2 cut(s) 95, 1383
BsmFI GGGAC 1 cut(s) 1185
BsmI GAATGC 1 cut(s) 1226
Bsp1286I GDGCHC 1 cut(s) 975
Bsp13I TCCGGA 1 cut(s) 592
Bsp143I GATC 3 cut(s) 600, 939, 961
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 1 cut(s) 132
BspCNI CTCAG 4 cut(s) 450, 544, 1335, 1360
BspEI TCCGGA 1 cut(s) 592
BspFNI CGCG 1 cut(s) 921
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 1 cut(s) 969
BsrDI GCAATG 1 cut(s) 872
BsrI ACTGG 1 cut(s) 267
BssMI GATC 3 cut(s) 600, 939, 961
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 900
Bst2BI CACGAG 1 cut(s) 900
Bst2UI CCWGG 1 cut(s) 1376
Bst4CI ACNGT 6 cut(s) 106, 666, 832, 928, 1037, 1130
Bst6I CTCTTC 3 cut(s) 40, 1071, 1389
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstBAI YACGTR 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 435, 975, 1308
BstDEI CTNAG 7 cut(s) 236, 429, 458, 552, 1094, 1322, 1368
BstF5I GGATG 3 cut(s) 655, 897, 1104
BstFNI CGCG 1 cut(s) 921
BstH2I RGCGCY 1 cut(s) 722
BstHHI GCGC 1 cut(s) 721
BstKTI GATC 3 cut(s) 603, 942, 964
BstMAI GTCTC 2 cut(s) 95, 1383
BstMBI GATC 3 cut(s) 600, 939, 961
BstMWI GCNNNNNNNGC 6 cut(s) 95, 365, 434, 443, 1276, 1307
BstNI CCWGG 1 cut(s) 1376
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 1374
BstSFI CTRYAG 3 cut(s) 736, 1033, 1299
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 1 cut(s) 1285
BstV2I GAAGAC 1 cut(s) 1075
BstXI CCANNNNNNTGG 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 1094
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 3 cut(s) 655, 897, 1104
BtsIMutI CAGTG 2 cut(s) 845, 870
Cac8I GCNNGC 3 cut(s) 435, 975, 1308
CfoI GCGC 1 cut(s) 721
Cfr13I GGNCC 4 cut(s) 806, 886, 1346, 1372
CseI GACGC 3 cut(s) 185, 910, 1193
Csp6I GTAC 4 cut(s) 712, 933, 1038, 1140
CviAII CATG 1 cut(s) 657
CviQI GTAC 4 cut(s) 712, 933, 1038, 1140
DdeI CTNAG 7 cut(s) 236, 429, 458, 552, 1094, 1322, 1368
DpnI GATC 3 cut(s) 602, 941, 963
DpnII GATC 3 cut(s) 600, 939, 961
DraI TTTAAA 1 cut(s) 400
DraIII CACNNNGTG 1 cut(s) 1291
Eam1104I CTCTTC 3 cut(s) 40, 1071, 1389
EarI CTCTTC 3 cut(s) 40, 1071, 1389
Eco47I GGWCC 4 cut(s) 806, 886, 1346, 1372
Eco57I CTGAAG 2 cut(s) 390, 1095
Eco81I CCTNAGG 1 cut(s) 1094
EcoO109I RGGNCCY 1 cut(s) 1372
EcoRII CCWGG 1 cut(s) 1374
EcoT22I ATGCAT 1 cut(s) 658
Esp3I CGTCTC 2 cut(s) 95, 1383
FaeI CATG 1 cut(s) 660
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 1185
FatI CATG 1 cut(s) 656
FauNDI CATATG 2 cut(s) 248, 1351
FbaI TGATCA 2 cut(s) 600, 939
Fnu4HI GCNGC 2 cut(s) 133, 1299
FokI GGATG 3 cut(s) 662, 904, 1111
Fsp4HI GCNGC 2 cut(s) 133, 1299
FspBI CTAG 1 cut(s) 152
GlaI GCGC 1 cut(s) 720
GluI GCNGC 2 cut(s) 133, 1299
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 2 cut(s) 250, 1407
HaeII RGCGCY 1 cut(s) 722
HapII CCGG 3 cut(s) 470, 593, 889
HgaI GACGC 3 cut(s) 185, 910, 1193
HhaI GCGC 1 cut(s) 721
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 1 cut(s) 660
Hin6I GCGC 1 cut(s) 719
HinP1I GCGC 1 cut(s) 719
HindIII AAGCTT 2 cut(s) 300, 431
HinfI GANTC 6 cut(s) 27, 112, 155, 732, 835, 1238
HpaII CCGG 3 cut(s) 470, 593, 889
HphI GGTGA 6 cut(s) 107, 548, 574, 625, 1214, 1390
Hpy166II GTNNAC 7 cut(s) 262, 662, 712, 911, 1140, 1183, 1205
Hpy188I TCNGA 3 cut(s) 459, 1243, 1369
Hpy188III TCNNGA 5 cut(s) 24, 109, 593, 725, 1386
Hpy8I GTNNAC 7 cut(s) 262, 662, 712, 911, 1140, 1183, 1205
HpyAV CCTTC 6 cut(s) 365, 526, 559, 673, 781, 785
HpyCH4III ACNGT 6 cut(s) 106, 666, 832, 928, 1037, 1130
HpyCH4IV ACGT 4 cut(s) 92, 605, 767, 1174
HpyCH4V TGCA 3 cut(s) 656, 1301, 1310
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 365, 434, 443, 1276, 1307
HpyF3I CTNAG 7 cut(s) 236, 429, 458, 552, 1094, 1322, 1368
HpySE526I ACGT 4 cut(s) 92, 605, 767, 1174
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 1 cut(s) 660
HspAI GCGC 1 cut(s) 719
Kpn2I TCCGGA 1 cut(s) 592
Ksp22I TGATCA 2 cut(s) 600, 939
Kzo9I GATC 3 cut(s) 600, 939, 961
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 1 cut(s) 1285
LweI GCATC 4 cut(s) 197, 643, 865, 1092
MaeI CTAG 1 cut(s) 152
MaeII ACGT 4 cut(s) 92, 605, 767, 1174
MaeIII GTNAC 5 cut(s) 305, 817, 848, 922, 1015
MalI GATC 3 cut(s) 602, 941, 963
MboI GATC 3 cut(s) 600, 939, 961
MboII GAAGA 7 cut(s) 6, 27, 1080, 1088, 1221, 1225, 1406
MfeI CAATTG 2 cut(s) 227, 1409
MhlI GDGCHC 1 cut(s) 975
MluCI AATT 7 cut(s) 42, 138, 169, 227, 1332, 1409, 1420
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 3 cut(s) 627, 753, 1045
Mph1103I ATGCAT 1 cut(s) 658
MroI TCCGGA 1 cut(s) 592
MslI CAYNNNNRTG 2 cut(s) 126, 608
MspI CCGG 3 cut(s) 470, 593, 889
MspR9I CCNGG 1 cut(s) 1376
MunI CAATTG 2 cut(s) 227, 1409
Mva1269I GAATGC 1 cut(s) 1226
MvaI CCWGG 1 cut(s) 1376
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 6 cut(s) 95, 365, 434, 443, 1276, 1307
NdeI CATATG 2 cut(s) 248, 1351
NdeII GATC 3 cut(s) 600, 939, 961
NlaIII CATG 1 cut(s) 660
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 2 cut(s) 305, 922
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 660
PctI GAATGC 1 cut(s) 1226
PfeI GAWTC 5 cut(s) 27, 112, 732, 835, 1238
PkrI GCNGC 2 cut(s) 134, 1300
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 1372
PsiI TTATAA 1 cut(s) 981
Psp5II RGGWCCY 1 cut(s) 1372
Psp6I CCWGG 1 cut(s) 1374
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 1 cut(s) 1374
PspPI GGNCC 4 cut(s) 806, 886, 1346, 1372
PspPPI RGGWCCY 1 cut(s) 1372
PstI CTGCAG 1 cut(s) 1303
RsaI GTAC 4 cut(s) 713, 934, 1039, 1141
RsaNI GTAC 4 cut(s) 712, 933, 1038, 1140
RseI CAYNNNNRTG 2 cut(s) 126, 608
SatI GCNGC 2 cut(s) 133, 1299
Sau3AI GATC 3 cut(s) 600, 939, 961
Sau96I GGNCC 4 cut(s) 806, 886, 1346, 1372
ScaI AGTACT 2 cut(s) 934, 1039
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 1376
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 4 cut(s) 197, 643, 865, 1092
SfcI CTRYAG 3 cut(s) 736, 1033, 1299
SinI GGWCC 4 cut(s) 806, 886, 1346, 1372
SmiMI CAYNNNNRTG 2 cut(s) 126, 608
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
Sse9I AATT 7 cut(s) 42, 138, 169, 227, 1332, 1409, 1420
SsiI CCGC 1 cut(s) 132
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 1374
TaaI ACNGT 6 cut(s) 106, 666, 832, 928, 1037, 1130
TaiI ACGT 4 cut(s) 95, 608, 770, 1177
TaqI TCGA 1 cut(s) 25
TaqII GACCGA 1 cut(s) 849
TasI AATT 7 cut(s) 42, 138, 169, 227, 1332, 1409, 1420
TatI WGTACW 3 cut(s) 711, 932, 1037
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 5 cut(s) 27, 112, 732, 835, 1238
TscAI CASTG 2 cut(s) 845, 870
TseFI GTSAC 2 cut(s) 305, 922
TseI GCWGC 1 cut(s) 1298
Tsp45I GTSAC 2 cut(s) 305, 922
TspDTI ATGAA 2 cut(s) 235, 692
TspRI CASTG 2 cut(s) 845, 870
VpaK11BI GGWCC 4 cut(s) 806, 886, 1346, 1372
XceI RCATGY 1 cut(s) 660
XspI CTAG 1 cut(s) 152
ZrmI AGTACT 2 cut(s) 934, 1039
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.