pycom06g04620

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008335
Physical Location & Seq
Forward (+)
6020697 .. 6022130
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04620.1

Sequence Viewer

Length: 1434 bp
ATGATGCGCCTTTCTTCAATCTCCCGGTTCGTCTCCTCCTCATTCGGCCTCAAAAACCACAAATTGTCAATCCTGCTCTTCTCTACGGCGGCGGCATCGGCTGAAACAGTGTATAATCACCTGCAGAAAAATGGTGGCAACGTAGAGGAAATCCTAGCGTCGATTCAGGTGAAATTGGACTCCAAATATGTAAGTCAGGTTTTACAGAGATGCAATGGTAGCCAATCACAAATGGGTCTTAGGTTTTTCATATGGGCTGGTCTTCAATCCACTTATAGGCACAGTTATTTTATGTATAGCAAAGCTTGTGAATTGTGTGAGATTAGGATAAAACCGACTGTGATTTTCGATGTTTTGGAGGCTTATAAAGTTGAAGGGTGCTTTGTCAATCTTAAGATGTTTAAGGTTGTTTTTAATTTGTGTAAAGAAGCTAAGCTTGCTGGTGAAGCTTTGAGGGTATTAAGGATAATGCCGGAATTTGGTTTGCGTCCAGATACTACGGTGTATAATATTGTTATAAGGTTGTTTTGTGATATGGAAGATATGGATATGGCTGAAAGTTTGGTGAAGGAGATGGGTTTGGTGGATCTTTGTCCTGATTTGATCACGTACATGGTATTGATCAAAGGGTTTTGTAATGTGGGTCGGTTAGATGACGCTTCTGGGTTGTTTAAGGTTATGAAGGAACATGGTTGTTTGCCCAATGCTGTGGTGTACTCGGCACTTCTTGATGGATTTTGTAGGTGTGATAATATGAAGAAAGCTTTTGAGTTATTGAAGGAAATGGAGAAGGAAGGTGGGGATTGTACTCCTAATGTTGTAACATATACATCCGTGATTCAAAAGCTTTGTGACAAGGGATTGTCGATGGATGCCTTGGTTATTTTGGACCGAATGGAAGCTTGTGGATGCGCCCCAAGTCGTGTTACAGTTAGTTCTCTTATTAAGGGTTTGTGTGTGGAGGATCGAGTTGAAGAGGTTTATAAGTTAATTGATAGAGTTGTTGCAGGAAGTAGTGTCAGTTATGGTGATTGCTATAGTTCTCTTGTTGTGTCCTTGGCAAAAGGTAGGAAACCTGAGGAGGCAGAAAAGGTCTTTAAGATGATGGTTGATGGCAGGGTCAAACCAAATAGTTTGGCCTGTAGCATTATGTTGAAAAAACTTTGTTTGGATGGTCGGGTGGTTGACGCTTTGTGCTTGTTTGATGAACTTAATAAAATGGAATGCTTGTCTTCCATCGACTCTGATGTTTATCCTATTCTTTTAATGGGACTTTGTCAGCAAATGCATTTAGTGGATGCTGCAAAGCTGGCAAAAACAATGCTAAAAAAAGGAATTAAACTGAAAGCTACTTACGTTGACAATATAGCTGAGATCCTGAAGAAATCCGGAGATGAGGAGTTAATTAAGAATTTGACTATAATTGCAAGGTGA

Protein Analysis

478

Amino Acids

53.17

Weight (kDa)

7.92

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 116 - 243 7.1e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 164 - 213 7.2e-11 PPR repeat family
PPR_3 PF13812 187 - 244 9.2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 197 - 228 9e-10 PPR repeat
PPR_2 PF13041 199 - 248 3.5e-15 PPR repeat family
PPR PF01535 202 - 232 7.3e-07 PPR repeat
PPR_long PF17177 219 - 334 4.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 231 - 263 9.2e-12 PPR repeat
PPR_2 PF13041 234 - 285 1.4e-14 PPR repeat family
PPR PF01535 237 - 266 7.4e-08 PPR repeat
PPR_3 PF13812 237 - 281 5.9e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 258 - 316 1.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 268 - 300 4.9e-08 PPR repeat
PPR_2 PF13041 271 - 319 5.3e-12 PPR repeat family
PPR_2 PF13041 346 - 389 2.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 366, 518, 984
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 129
AccIII TCCGGA 1 cut(s) 1388
AciI CCGC 2 cut(s) 89, 92
AclWI GGATC 3 cut(s) 594, 972, 1369
AcsI RAATTY 2 cut(s) 476, 1411
AcuI CTGAAG 1 cut(s) 1400
AfaI GTAC 3 cut(s) 611, 716, 808
AfiI CCNNNNNNNGG 2 cut(s) 276, 479
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 7 cut(s) 18, 266, 374, 778, 842, 974, 1156
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 3 cut(s) 594, 972, 1369
Aor13HI TCCGGA 1 cut(s) 1388
AoxI GGCC 2 cut(s) 46, 1137
ApeKI GCWGC 1 cut(s) 1301
ApoI RAATTY 2 cut(s) 476, 1411
AspLEI GCGC 2 cut(s) 9, 914
AspS9I GGNCC 1 cut(s) 889
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 5 cut(s) 110, 181, 455, 577, 1040
AvaII GGWCC 1 cut(s) 889
AxyI CCTNAGG 1 cut(s) 1077
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BbsI GAAGAC 2 cut(s) 254, 1224
BbvI GCAGC 1 cut(s) 1288
BccI CCATC 7 cut(s) 568, 725, 862, 1099, 1106, 1166, 1244
BceAI ACGGC 1 cut(s) 102
BclI TGATCA 2 cut(s) 603, 621
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 3 cut(s) 122, 1036, 1141
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 129
BisI GCNGC 3 cut(s) 90, 93, 1302
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 3 cut(s) 91, 94, 1303
Bme1390I CCNGG 1 cut(s) 25
Bme18I GGWCC 1 cut(s) 889
BmgT120I GGNCC 1 cut(s) 889
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 5 cut(s) 104, 200, 862, 899, 1288
BpiI GAAGAC 2 cut(s) 254, 1224
Bpu1102I GCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 1251
BsaJI CCNNGG 2 cut(s) 876, 1056
BsaWI WCCGGW 1 cut(s) 1388
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 479
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 1251
BseAI TCCGGA 1 cut(s) 1388
BseDI CCNNGG 2 cut(s) 876, 1056
BseGI GGATG 5 cut(s) 830, 877, 914, 1177, 1303
BseJI GATNNNNATC 1 cut(s) 1251
BseLI CCNNNNNNNGG 2 cut(s) 276, 479
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 1068, 1362
BseRI GAGGAG 4 cut(s) 25, 28, 1094, 1412
BseXI GCAGC 1 cut(s) 1288
BshFI GGCC 2 cut(s) 48, 1139
BsiSI CCGG 3 cut(s) 25, 473, 1389
BslFI GGGAC 1 cut(s) 1284
BslI CCNNNNNNNGG 2 cut(s) 276, 479
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 1284
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 2 cut(s) 48, 1139
Bsp13I TCCGGA 1 cut(s) 1388
Bsp143I GATC 5 cut(s) 586, 603, 621, 964, 1374
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 89, 92
BspANI GGCC 2 cut(s) 48, 1139
BspCNI CTCAG 2 cut(s) 1069, 1363
BspEI TCCGGA 1 cut(s) 1388
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 129
BspPI GGATC 3 cut(s) 594, 972, 1369
BspQI GCTCTTC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 876, 1056
BssMI GATC 5 cut(s) 586, 603, 621, 964, 1374
BssT1I CCWWGG 2 cut(s) 876, 1056
Bst4CI ACNGT 5 cut(s) 109, 284, 340, 502, 931
Bst6I CTCTTC 2 cut(s) 83, 969
BstAFI CTTAAG 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 438, 1311
BstDEI CTNAG 4 cut(s) 239, 432, 1077, 1371
BstF5I GGATG 5 cut(s) 830, 877, 914, 1177, 1303
BstHHI GCGC 2 cut(s) 9, 914
BstKTI GATC 5 cut(s) 589, 606, 624, 967, 1377
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 5 cut(s) 586, 603, 621, 964, 1374
BstMWI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
BstSCI CCNGG 1 cut(s) 23
BstSFI CTRYAG 3 cut(s) 122, 1036, 1141
BstV1I GCAGC 1 cut(s) 1288
BstV2I GAAGAC 2 cut(s) 254, 1224
BstX2I RGATCY 2 cut(s) 586, 1374
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 2 cut(s) 586, 1374
Bsu36I CCTNAGG 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 48, 1139
BtsCI GGATG 5 cut(s) 830, 877, 914, 1177, 1303
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 438, 1311
CfoI GCGC 2 cut(s) 9, 914
Cfr13I GGNCC 1 cut(s) 889
CseI GACGC 4 cut(s) 147, 476, 665, 1196
Csp6I GTAC 3 cut(s) 610, 715, 807
CviAII CATG 2 cut(s) 613, 689
CviQI GTAC 3 cut(s) 610, 715, 807
DdeI CTNAG 4 cut(s) 239, 432, 1077, 1371
DpnI GATC 5 cut(s) 588, 605, 623, 966, 1376
DpnII GATC 5 cut(s) 586, 603, 621, 964, 1374
Eam1104I CTCTTC 2 cut(s) 83, 969
EarI CTCTTC 2 cut(s) 83, 969
Eco130I CCWWGG 2 cut(s) 876, 1056
Eco47I GGWCC 1 cut(s) 889
Eco57I CTGAAG 1 cut(s) 1400
Eco81I CCTNAGG 1 cut(s) 1077
EcoT14I CCWWGG 2 cut(s) 876, 1056
EcoT22I ATGCAT 1 cut(s) 1290
ErhI CCWWGG 2 cut(s) 876, 1056
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 2 cut(s) 616, 692
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 1284
FatI CATG 2 cut(s) 612, 688
FauNDI CATATG 1 cut(s) 251
FbaI TGATCA 2 cut(s) 603, 621
Fnu4HI GCNGC 3 cut(s) 90, 93, 1302
FokI GGATG 5 cut(s) 817, 884, 921, 1184, 1310
Fsp4HI GCNGC 3 cut(s) 90, 93, 1302
FspBI CTAG 1 cut(s) 155
GlaI GCGC 2 cut(s) 8, 913
GluI GCNGC 3 cut(s) 90, 93, 1302
HaeIII GGCC 2 cut(s) 48, 1139
HapII CCGG 3 cut(s) 25, 473, 1389
HgaI GACGC 4 cut(s) 147, 476, 665, 1196
HhaI GCGC 2 cut(s) 9, 914
Hin1II CATG 2 cut(s) 616, 692
Hin6I GCGC 2 cut(s) 7, 912
HinP1I GCGC 2 cut(s) 7, 912
HincII GTYRAC 2 cut(s) 1186, 1360
HindII GTYRAC 2 cut(s) 1186, 1360
HindIII AAGCTT 6 cut(s) 303, 434, 447, 762, 845, 900
HinfI GANTC 4 cut(s) 163, 179, 838, 1241
HpaII CCGG 3 cut(s) 25, 473, 1389
HphI GGTGA 5 cut(s) 110, 181, 455, 577, 1040
Hpy166II GTNNAC 3 cut(s) 715, 1186, 1360
Hpy188I TCNGA 1 cut(s) 1246
Hpy188III TCNNGA 5 cut(s) 491, 596, 728, 1378, 1389
Hpy8I GTNNAC 3 cut(s) 715, 1186, 1360
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 6 cut(s) 368, 562, 676, 772, 784, 788
HpyCH4III ACNGT 5 cut(s) 109, 284, 340, 502, 931
HpyCH4IV ACGT 3 cut(s) 141, 608, 1356
HpyCH4V TGCA 6 cut(s) 124, 213, 1007, 1288, 1304, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
HpyF3I CTNAG 4 cut(s) 239, 432, 1077, 1371
HpySE526I ACGT 3 cut(s) 141, 608, 1356
Hsp92II CATG 2 cut(s) 616, 692
HspAI GCGC 2 cut(s) 7, 912
Kpn2I TCCGGA 1 cut(s) 1388
Ksp22I TGATCA 2 cut(s) 603, 621
Kzo9I GATC 5 cut(s) 586, 603, 621, 964, 1374
LguI GCTCTTC 1 cut(s) 83
Lsp1109I GCAGC 1 cut(s) 1288
LweI GCATC 5 cut(s) 104, 200, 862, 899, 1288
MaeI CTAG 1 cut(s) 155
MaeII ACGT 3 cut(s) 141, 608, 1356
MaeIII GTNAC 3 cut(s) 820, 851, 925
MalI GATC 5 cut(s) 588, 605, 623, 966, 1376
MboI GATC 5 cut(s) 586, 603, 621, 964, 1374
MboII GAAGA 8 cut(s) 6, 70, 254, 551, 769, 986, 1224, 1393
MflI RGATCY 2 cut(s) 586, 1374
MlyI GAGTC 2 cut(s) 173, 1235
Mph1103I ATGCAT 1 cut(s) 1290
MroI TCCGGA 1 cut(s) 1388
MslI CAYNNNNRTG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 3 cut(s) 25, 473, 1389
MspR9I CCNGG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 1229
MwoI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
NciI CCSGG 1 cut(s) 25
NdeI CATATG 1 cut(s) 251
NdeII GATC 5 cut(s) 586, 603, 621, 964, 1374
NlaIII CATG 2 cut(s) 616, 692
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 851
NsiI ATGCAT 1 cut(s) 1290
PacI TTAATTAA 1 cut(s) 1407
PaqCI CACCTGC 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 83
PctI GAATGC 1 cut(s) 1229
PfeI GAWTC 2 cut(s) 163, 838
PflFI GACNNNGTC 1 cut(s) 1275
PkrI GCNGC 3 cut(s) 91, 94, 1303
PleI GAGTC 2 cut(s) 173, 1235
PpsI GAGTC 2 cut(s) 173, 1235
Ppu21I YACGTR 1 cut(s) 609
PsiI TTATAA 3 cut(s) 366, 518, 984
PspPI GGNCC 1 cut(s) 889
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 2 cut(s) 586, 1374
PsyI GACNNNGTC 1 cut(s) 1275
RsaI GTAC 3 cut(s) 611, 716, 808
RsaNI GTAC 3 cut(s) 610, 715, 807
RseI CAYNNNNRTG 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 83
SatI GCNGC 3 cut(s) 90, 93, 1302
Sau3AI GATC 5 cut(s) 586, 603, 621, 964, 1374
Sau96I GGNCC 1 cut(s) 889
SchI GAGTC 2 cut(s) 173, 1235
ScrFI CCNGG 1 cut(s) 25
SfaNI GCATC 5 cut(s) 104, 200, 862, 899, 1288
SfcI CTRYAG 3 cut(s) 122, 1036, 1141
SinI GGWCC 1 cut(s) 889
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SsiI CCGC 2 cut(s) 89, 92
SspI AATATT 1 cut(s) 511
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 23
StyI CCWWGG 2 cut(s) 876, 1056
TaaI ACNGT 5 cut(s) 109, 284, 340, 502, 931
TaiI ACGT 3 cut(s) 144, 611, 1359
TaqI TCGA 5 cut(s) 161, 348, 866, 967, 1239
TaqII GACCGA 1 cut(s) 906
TatI WGTACW 2 cut(s) 714, 806
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 2 cut(s) 163, 838
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 1 cut(s) 1301
Tsp45I GTSAC 1 cut(s) 851
TspDTI ATGAA 4 cut(s) 238, 695, 770, 1221
TspGWI ACGGA 1 cut(s) 823
TspRI CASTG 1 cut(s) 114
Tth111I GACNNNGTC 1 cut(s) 1275
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 889
XapI RAATTY 2 cut(s) 476, 1411
XspI CTAG 1 cut(s) 155
Zsp2I ATGCAT 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.