MD06G1055200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008335
Physical Location & Seq
Forward (+)
8234893 .. 8238002
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1055200.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGATGAGCCTTTCTTCAATCTCCCGGTTCGTCTCCTCCTCTTTCGGCCTCAAAAATCACAAATTGTCAATCCTGCAGTTCTCTACGGCGGCGGCATCGGCTGAAACAGTGTACAATCACCTGCAGATAAATGGTGGCAACGTAGAGGAAATCCTAGCGTCGATTCAGGTGAAATTGGACTCCAAATATGTAAGTCAGGTTTTACAGAGATGCAATGGTAGCCAATCACAAATGGGTCTTAGGTTTTTCATATGGGCTGGTCTTCAATCCACTTATAGGCACAGTTATTTTATGTATAGCAAAGCTTGTGAATTGTGTGAGATTAGGATAAAACCGACTGTGATTTTCGATGTTTTGGAGGCTTATAAAGTTGAAGGTTGCTTTGTTAATCTTAAGATGTTTAAGGTTGTTTTTAATTTGTGTAAAGAAGCTAAGCTTGCTGATGAAGCTTTGAGGGTATTAAGGATAATGCCGGAATTTGGTTTGCGTCCAGATACTACGGTGTATAATGTTATTATAAGGTTGTTTTGTGATATGGAAGATATGGATATGGCTGAAAGTTTGGTGAAGGAGATGGATTTGGTGGATCTTTGTCCCGATTTGATCACGTACATGGTATTGATCAAAGGGTTTTGTAATGTGGGTCGGTTAGATGATGCTTGTGGGTTGTTTAAGGTTATGAAGGAACATGGTTGTTTGCCTAATGCTGTGGTGTACTCGGCACTTCTTGATGGATTTTGTAGGTGTGATAACATGAAGAGAGCTTTAGAGTTATTGAAGGAAATGGAGAAGGAAGGTGGGGATTGTACTCCTAATGTAGTTACATATACATCTGTGATTCAAAAGCTTTGTGACAAGGGATTGTCGACGGATGCCTTGGTTATTTTGGACCGAATGGAAGCTTGTGGATGTGCCCCAAGTCGCGTTACAGTTAGTTCTCTTATTAAGGGTTTGTGTGTGGAAGATCGAGTTGAAGAGGCTTATAAGTTAATTGATAAAGTTGTTGCAGGAAGTAGTGTCAGCTATGGTGATTGCTATAGTTCTCTTGTTGTGTCCTTGGCAAGAGGTAGGAAACCTGAGGAGGCAGAAAAGGTCTTTAAGATGATGGTTGATGGCAGGATCAAACCAAATAGTTTGGCCTGTAGCATTATGTTGAAAAAACTTTGTTTGGATGGTCGGGTGGTTGACACTTTGTGCTTGTTTGATGAACTTAATAAAATGGAATGCTTGTCTTCCATCGACTCTGATGTTTATCCTGTTCTTTTAATGGGACTTTGTCAGCAAATGCATTTAGTGGATGCTGCAAAGCTAGCGAAAACAATGCTAAAAAAAGGATTTAAACTGAAAGCTCCTTACGTTGACAATATAGCTGAGATCCTGAAGAAATCTGGAGATGAGGAGTTAATTAAGAATTTGACTATAATTGCAAGGTGA

Protein Analysis

478

Amino Acids

53.23

Weight (kDa)

6.56

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 116 - 243 3.2e-13 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 164 - 213 3.2e-11 PPR repeat family
PPR_3 PF13812 187 - 244 4.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 197 - 228 5.9e-10 PPR repeat
PPR_2 PF13041 199 - 248 1.2e-15 PPR repeat family
PPR PF01535 202 - 232 4.6e-07 PPR repeat
PPR_long PF17177 223 - 334 2.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 231 - 263 6.1e-11 PPR repeat
PPR_2 PF13041 234 - 285 9.2e-14 PPR repeat family
PPR_3 PF13812 234 - 281 7.4e-07 Pentatricopeptide repeat domain
PPR PF01535 237 - 266 2.2e-07 PPR repeat
PPR_1 PF12854 268 - 300 1.1e-07 PPR repeat
PPR_3 PF13812 270 - 316 4.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 271 - 319 3.2e-12 PPR repeat family
PPR_1 PF12854 303 - 333 9.2e-07 PPR repeat
PPR_2 PF13041 346 - 389 3.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 366, 518, 984
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 129
AccI GTMKAC 1 cut(s) 866
AccII CGCG 1 cut(s) 924
AciI CCGC 2 cut(s) 89, 92
AclWI GGATC 3 cut(s) 594, 1127, 1369
AcsI RAATTY 2 cut(s) 476, 1411
AcuI CTGAAG 1 cut(s) 1400
AdeI CACNNNGTG 1 cut(s) 1194
AfaI GTAC 4 cut(s) 113, 611, 716, 808
AfiI CCNNNNNNNGG 2 cut(s) 276, 479
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 7 cut(s) 18, 266, 374, 778, 842, 974, 1156
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 3 cut(s) 594, 1127, 1369
AoxI GGCC 2 cut(s) 46, 1137
ApeKI GCWGC 1 cut(s) 1301
ApoI RAATTY 2 cut(s) 476, 1411
AspS9I GGNCC 1 cut(s) 889
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 4 cut(s) 110, 181, 577, 1040
AsuNHI GCTAGC 1 cut(s) 1309
AvaII GGWCC 1 cut(s) 889
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 1 cut(s) 916
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BbsI GAAGAC 2 cut(s) 254, 1224
BbvI GCAGC 1 cut(s) 1288
BccI CCATC 6 cut(s) 568, 725, 1099, 1106, 1166, 1244
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 1303, 1337
BclI TGATCA 2 cut(s) 603, 621
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 2 cut(s) 155, 1310
BfmI CTRYAG 4 cut(s) 74, 122, 1036, 1141
BfrI CTTAAG 1 cut(s) 392
BfuAI ACCTGC 1 cut(s) 129
BisI GCNGC 3 cut(s) 90, 93, 1302
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 3 cut(s) 91, 94, 1303
Bme1390I CCNGG 1 cut(s) 25
Bme18I GGWCC 1 cut(s) 889
BmgT120I GGNCC 1 cut(s) 889
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 5 cut(s) 104, 200, 646, 862, 1288
BmtI GCTAGC 1 cut(s) 1313
BpiI GAAGAC 2 cut(s) 254, 1224
BpmI CTGGAG 1 cut(s) 1410
Bpu1102I GCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 1251
BsaJI CCNNGG 2 cut(s) 876, 1056
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 479
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 1251
BseDI CCNNGG 2 cut(s) 876, 1056
BseGI GGATG 4 cut(s) 877, 914, 1177, 1303
BseJI GATNNNNATC 1 cut(s) 1251
BseLI CCNNNNNNNGG 2 cut(s) 276, 479
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 1068, 1362
BseRI GAGGAG 4 cut(s) 25, 28, 1094, 1412
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 1 cut(s) 1288
Bsh1236I CGCG 1 cut(s) 924
BshFI GGCC 2 cut(s) 48, 1139
BsiSI CCGG 2 cut(s) 25, 473
BslFI GGGAC 2 cut(s) 579, 1284
BslI CCNNNNNNNGG 2 cut(s) 276, 479
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 2 cut(s) 579, 1284
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 2 cut(s) 48, 1139
Bsp1286I GDGCHC 1 cut(s) 916
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 89, 92
BspANI GGCC 2 cut(s) 48, 1139
BspCNI CTCAG 2 cut(s) 1069, 1363
BspFNI CGCG 1 cut(s) 924
BspMAI CTGCAG 2 cut(s) 78, 126
BspMI ACCTGC 1 cut(s) 129
BspOI GCTAGC 1 cut(s) 1313
BspPI GGATC 3 cut(s) 594, 1127, 1369
BspTI CTTAAG 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 220
BsrGI TGTACA 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 876, 1056
BssMI GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
BssT1I CCWWGG 2 cut(s) 876, 1056
Bst4CI ACNGT 5 cut(s) 109, 284, 340, 502, 931
Bst6I CTCTTC 2 cut(s) 752, 969
BstAFI CTTAAG 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 438, 1311
BstDEI CTNAG 4 cut(s) 239, 432, 1077, 1371
BstF5I GGATG 4 cut(s) 877, 914, 1177, 1303
BstFNI CGCG 1 cut(s) 924
BstKTI GATC 6 cut(s) 589, 606, 624, 967, 1122, 1377
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
BstMWI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
BstSCI CCNGG 1 cut(s) 23
BstSFI CTRYAG 4 cut(s) 74, 122, 1036, 1141
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 1 cut(s) 1288
BstV2I GAAGAC 2 cut(s) 254, 1224
BstX2I RGATCY 2 cut(s) 586, 1374
BstYI RGATCY 2 cut(s) 586, 1374
Bsu36I CCTNAGG 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 48, 1139
BtsCI GGATG 4 cut(s) 877, 914, 1177, 1303
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 438, 1311
Cfr13I GGNCC 1 cut(s) 889
CseI GACGC 2 cut(s) 147, 476
Csp6I GTAC 4 cut(s) 112, 610, 715, 807
CviAII CATG 3 cut(s) 613, 689, 754
CviQI GTAC 4 cut(s) 112, 610, 715, 807
DdeI CTNAG 4 cut(s) 239, 432, 1077, 1371
DpnI GATC 6 cut(s) 588, 605, 623, 966, 1121, 1376
DpnII GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
DraI TTTAAA 1 cut(s) 1339
DraIII CACNNNGTG 1 cut(s) 1194
Eam1104I CTCTTC 2 cut(s) 752, 969
EarI CTCTTC 2 cut(s) 752, 969
Eco130I CCWWGG 2 cut(s) 876, 1056
Eco47I GGWCC 1 cut(s) 889
Eco57I CTGAAG 1 cut(s) 1400
Eco81I CCTNAGG 1 cut(s) 1077
EcoT14I CCWWGG 2 cut(s) 876, 1056
EcoT22I ATGCAT 1 cut(s) 1290
ErhI CCWWGG 2 cut(s) 876, 1056
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 3 cut(s) 616, 692, 757
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 2 cut(s) 579, 1284
FatI CATG 3 cut(s) 612, 688, 753
FauNDI CATATG 1 cut(s) 251
FbaI TGATCA 2 cut(s) 603, 621
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 3 cut(s) 90, 93, 1302
FokI GGATG 4 cut(s) 884, 921, 1184, 1310
Fsp4HI GCNGC 3 cut(s) 90, 93, 1302
FspBI CTAG 2 cut(s) 155, 1310
GluI GCNGC 3 cut(s) 90, 93, 1302
GsuI CTGGAG 1 cut(s) 1410
HaeIII GGCC 2 cut(s) 48, 1139
HapII CCGG 2 cut(s) 25, 473
HgaI GACGC 2 cut(s) 147, 476
Hin1II CATG 3 cut(s) 616, 692, 757
HincII GTYRAC 3 cut(s) 867, 1186, 1360
HindII GTYRAC 3 cut(s) 867, 1186, 1360
HindIII AAGCTT 5 cut(s) 303, 434, 447, 845, 900
HinfI GANTC 4 cut(s) 163, 179, 838, 1241
HpaII CCGG 2 cut(s) 25, 473
HphI GGTGA 4 cut(s) 110, 181, 577, 1040
Hpy166II GTNNAC 5 cut(s) 112, 715, 867, 1186, 1360
Hpy188I TCNGA 1 cut(s) 1246
Hpy188III TCNNGA 5 cut(s) 491, 596, 728, 1378, 1389
Hpy8I GTNNAC 5 cut(s) 112, 715, 867, 1186, 1360
Hpy99I CGWCG 2 cut(s) 163, 871
HpyAV CCTTC 6 cut(s) 368, 562, 676, 772, 784, 788
HpyCH4III ACNGT 5 cut(s) 109, 284, 340, 502, 931
HpyCH4IV ACGT 3 cut(s) 141, 608, 1356
HpyCH4V TGCA 7 cut(s) 76, 124, 213, 1007, 1288, 1304, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
HpyF3I CTNAG 4 cut(s) 239, 432, 1077, 1371
HpySE526I ACGT 3 cut(s) 141, 608, 1356
Hsp92II CATG 3 cut(s) 616, 692, 757
Ksp22I TGATCA 2 cut(s) 603, 621
Kzo9I GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
LmnI GCTCC 1 cut(s) 1354
Lsp1109I GCAGC 1 cut(s) 1288
LweI GCATC 5 cut(s) 104, 200, 646, 862, 1288
MaeI CTAG 2 cut(s) 155, 1310
MaeII ACGT 3 cut(s) 141, 608, 1356
MaeIII GTNAC 3 cut(s) 820, 851, 925
MalI GATC 6 cut(s) 588, 605, 623, 966, 1121, 1376
MboI GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
MboII GAAGA 8 cut(s) 6, 254, 551, 769, 974, 986, 1224, 1393
MflI RGATCY 2 cut(s) 586, 1374
MhlI GDGCHC 1 cut(s) 916
MluCI AATT 9 cut(s) 62, 173, 311, 415, 476, 990, 1404, 1411, 1422
MlyI GAGTC 2 cut(s) 173, 1235
Mph1103I ATGCAT 1 cut(s) 1290
MslI CAYNNNNRTG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 2 cut(s) 25, 473
MspR9I CCNGG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 1229
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 5 cut(s) 98, 219, 437, 446, 1310
NciI CCSGG 1 cut(s) 25
NdeI CATATG 1 cut(s) 251
NdeII GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
NheI GCTAGC 1 cut(s) 1309
NlaIII CATG 3 cut(s) 616, 692, 757
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 851
NsiI ATGCAT 1 cut(s) 1290
PacI TTAATTAA 1 cut(s) 1407
PaqCI CACCTGC 1 cut(s) 129
PctI GAATGC 1 cut(s) 1229
PfeI GAWTC 2 cut(s) 163, 838
PflFI GACNNNGTC 1 cut(s) 1275
PkrI GCNGC 3 cut(s) 91, 94, 1303
PleI GAGTC 2 cut(s) 173, 1235
PpsI GAGTC 2 cut(s) 173, 1235
Ppu21I YACGTR 1 cut(s) 609
PsiI TTATAA 3 cut(s) 366, 518, 984
PspPI GGNCC 1 cut(s) 889
PstI CTGCAG 2 cut(s) 78, 126
PsuI RGATCY 2 cut(s) 586, 1374
PsyI GACNNNGTC 1 cut(s) 1275
RsaI GTAC 4 cut(s) 113, 611, 716, 808
RsaNI GTAC 4 cut(s) 112, 610, 715, 807
RseI CAYNNNNRTG 1 cut(s) 611
SalI GTCGAC 1 cut(s) 865
SatI GCNGC 3 cut(s) 90, 93, 1302
Sau3AI GATC 6 cut(s) 586, 603, 621, 964, 1119, 1374
Sau96I GGNCC 1 cut(s) 889
SchI GAGTC 2 cut(s) 173, 1235
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 5 cut(s) 104, 200, 646, 862, 1288
SfcI CTRYAG 4 cut(s) 74, 122, 1036, 1141
SinI GGWCC 1 cut(s) 889
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 9 cut(s) 62, 173, 311, 415, 476, 990, 1404, 1411, 1422
SsiI CCGC 2 cut(s) 89, 92
SspMI CTAG 2 cut(s) 155, 1310
StyD4I CCNGG 1 cut(s) 23
StyI CCWWGG 2 cut(s) 876, 1056
TaaI ACNGT 5 cut(s) 109, 284, 340, 502, 931
TaiI ACGT 3 cut(s) 144, 611, 1359
TaqI TCGA 5 cut(s) 161, 348, 866, 967, 1239
TaqII GACCGA 1 cut(s) 906
TasI AATT 9 cut(s) 62, 173, 311, 415, 476, 990, 1404, 1411, 1422
TatI WGTACW 3 cut(s) 111, 714, 806
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 2 cut(s) 163, 838
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 1 cut(s) 1301
Tsp45I GTSAC 1 cut(s) 851
TspDTI ATGAA 5 cut(s) 238, 459, 695, 770, 1221
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 1 cut(s) 114
Tth111I GACNNNGTC 1 cut(s) 1275
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 889
XapI RAATTY 2 cut(s) 476, 1411
XmiI GTMKAC 1 cut(s) 866
XspI CTAG 2 cut(s) 155, 1310
Zsp2I ATGCAT 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.