Rw7G018430

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008335
Physical Location & Seq
Forward (+)
19923415 .. 19924844
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018430.1

Sequence Viewer

Length: 1401 bp
ATGACGAACCTTTCTTCAATCTCTCGGTTCCTCTCTTCCTCAATTCGCCTCAAAAATCACAAGCTCTCAATTTCTCACTTCTCTACTGTCTCGTCGGCAGAGACGGTCTACAATCACCTCCAGAGAAATGGTGGCAACATTGAGAAAACCCTAGCCTCAATGAGGCTCAATTTGGACTCCAAATGTGTAAGTCAGGTTTTACATAGATGCTATCCCACCCAATCCCAAATGGGTCTTAGATTTTTCATATGGGCTGGGGTTCACTCCAGTTATAGGCATAGCTATTTTATGTTCAGCAAAGCTTGTGAATTGTATAGGATTAGGCAAAACCCAAGTGTGATTTTTGATGTTTTGGAGGCTTATAGTGCTGAAGGTTGCTCTGTTAATATGAAAATGTTTAAACTTGCTGATGAGGCTTTGAGGGTTCTGAGGAAAATGCCGGAGTTCGGGTTGCGAGGGGATAATGTAGTGTATAATGTTGTTATAAGGTTGTTTTGTGATAAGGGTGATTTGGATATGGCGGAGAGCTTGGTGAAGGAGATGAGCATGGTGGAGCTTTATCCGGATTTGATCACATACATGGTGATGATTAAAGGGTTGTGTAATGTGGGTCGGTTGGATGATGCGTGTGGGTTGTTTAAGTTTATGAAGGAAAATGGTTGTTTGCCTAATGTTGTGGTGTACTCGGCCCTTCTCGATGGATTCTGTAGGTTTGGGGATATGGAGAGAGCCTTGAAGTTGTTAGAGGAGATGGAGAAGGAAGGTGGGGATTGTAGTCCTAATGTAGTAACATATACGTCTGTGATTCAATATCTTTGTGACAAGGGTCGGTCAGTGGAGGCATTGCTTGTTTTGGACCGAATGGAAGTTCGTGGTTGTTTACCAACTCGCGTCACGGTTAGTTCTTTGATCACAGGTTTTGTTAAGGAGGATCAAGTTGAGCAGGCTTATAAGTTGGTTGATAGAGTTGTTGAAAGTGGAAGTGTTACAAAAACTGATTGCTATAGTTCTCTTGTTGTGTCTTTGGAAAGAGTCAGAAAACCCGAGGAGGCAGAGAAAGTCTTTAGGACGATGCTTGATAGTGGGGTCAAACCAAACAGTTTGGTGTCTACCATTATGTTAAAAAAACGTTGTTTGGAGGGACGTATGGTGGATGCATATTGCTTGTTTGATGAACTTGAGAAGATGGAATGCTTATCTTCTATTGATTCTGATACTTATTCTATTCTTTTAGTGGGGCTTTGTCAGCAAAGACACTTAGTGCAGGCTGCAAAGCTTGCAAGGGTAATGCTGAGTAAAGGAATTAAACTAAAAGCTCCATATGTTGATATTATAGCTGAGGTCCTGATGAAATCTGGAGATGAGGAGTTACTTGAACAATTGACAAGAAATGGAAGGTGA

Protein Analysis

466

Amino Acids

52.66

Weight (kDa)

7.79

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 133 - 232 1.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 154 - 202 3e-12 PPR repeat family
PPR_3 PF13812 176 - 233 1.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 186 - 217 1.6e-08 PPR repeat
PPR_2 PF13041 188 - 237 7.2e-16 PPR repeat family
PPR PF01535 191 - 221 3.2e-07 PPR repeat
PPR_long PF17177 212 - 326 7.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 219 - 252 7.5e-13 PPR repeat
PPR_2 PF13041 223 - 255 9.7e-09 PPR repeat family
PPR_3 PF13812 223 - 271 2.6e-08 Pentatricopeptide repeat domain
PPR PF01535 226 - 255 2.5e-08 PPR repeat
PPR_3 PF13812 246 - 309 6.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 257 - 289 2.4e-08 PPR repeat
PPR_2 PF13041 260 - 309 3.3e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 485, 951
AccI GTMKAC 2 cut(s) 108, 1109
AccII CGCG 1 cut(s) 891
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 1 cut(s) 521
AclI AACGTT 1 cut(s) 1129
AclWI GGATC 1 cut(s) 939
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 1261
AfaI GTAC 1 cut(s) 683
AfiI CCNNNNNNNGG 3 cut(s) 162, 273, 446
AgsI TTSAA 5 cut(s) 18, 736, 809, 974, 1376
AluBI AGCT 8 cut(s) 64, 282, 302, 528, 556, 1276, 1316, 1337
AluI AGCT 8 cut(s) 64, 282, 302, 528, 556, 1276, 1316, 1337
Alw26I GTCTC 2 cut(s) 94, 95
AlwI GGATC 1 cut(s) 939
Ama87I CYCGRG 1 cut(s) 1043
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 687
ApeKI GCWGC 1 cut(s) 1268
AspS9I GGNCC 3 cut(s) 688, 856, 1342
AsuHPI GGTGA 4 cut(s) 107, 518, 544, 595
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 2 cut(s) 856, 1342
BbvCI CCTCAGC 1 cut(s) 1338
BbvI GCAGC 1 cut(s) 1255
BccI CCATC 3 cut(s) 692, 745, 1180
BclI TGATCA 2 cut(s) 570, 909
BcoDI GTCTC 2 cut(s) 94, 95
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 2 cut(s) 706, 1003
BisI GCNGC 1 cut(s) 1269
BlsI GCNGC 1 cut(s) 1270
Bme18I GGWCC 2 cut(s) 856, 1342
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 3 cut(s) 688, 856, 1342
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 4 cut(s) 197, 613, 1062, 1144
BoxI GACNNNNGTC 1 cut(s) 825
BpmI CTGGAG 3 cut(s) 104, 250, 1377
Bpu10I CCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 1 cut(s) 1199
BsaJI CCNNGG 1 cut(s) 1044
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 273, 446
Bse1I ACTGG 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 842
BseAI TCCGGA 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 1044
BseGI GGATG 2 cut(s) 625, 1159
BseLI CCNNNNNNNGG 3 cut(s) 162, 273, 446
BseMI GCAATG 1 cut(s) 842
BseMII CTCAG 3 cut(s) 419, 1283, 1329
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 3 cut(s) 761, 1061, 1379
BseXI GCAGC 1 cut(s) 1255
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 1283
Bsh1236I CGCG 1 cut(s) 891
BshFI GGCC 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 2 cut(s) 440, 563
BslFI GGGAC 1 cut(s) 1155
BslI CCNNNNNNNGG 3 cut(s) 162, 273, 446
BsmAI GTCTC 2 cut(s) 94, 95
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 1155
BsmI GAATGC 1 cut(s) 1196
BsnI GGCC 1 cut(s) 689
BsoBI CYCGRG 1 cut(s) 1043
Bsp13I TCCGGA 1 cut(s) 562
Bsp143I GATC 3 cut(s) 570, 909, 931
BspACI CCGC 1 cut(s) 521
BspANI GGCC 1 cut(s) 689
BspCNI CTCAG 3 cut(s) 420, 1284, 1330
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 939
BsrDI GCAATG 1 cut(s) 842
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 1044
BssMI GATC 3 cut(s) 570, 909, 931
Bst4CI ACNGT 4 cut(s) 88, 106, 898, 1100
Bst6I CTCTTC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstC8I GCNNGC 3 cut(s) 945, 1266, 1278
BstDEI CTNAG 5 cut(s) 236, 428, 1258, 1292, 1338
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 625, 1159
BstFNI CGCG 1 cut(s) 891
BstKTI GATC 3 cut(s) 573, 912, 934
BstMAI GTCTC 2 cut(s) 94, 95
BstMBI GATC 3 cut(s) 570, 909, 931
BstMWI GCNNNNNNNGC 4 cut(s) 365, 413, 1246, 1277
BstPAI GACNNNNGTC 1 cut(s) 825
BstSFI CTRYAG 2 cut(s) 706, 1003
BstUI CGCG 1 cut(s) 891
BstV1I GCAGC 1 cut(s) 1255
BstXI CCANNNNNNTGG 1 cut(s) 128
BsuRI GGCC 1 cut(s) 689
BtsCI GGATG 2 cut(s) 625, 1159
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 3 cut(s) 945, 1266, 1278
Cfr13I GGNCC 3 cut(s) 688, 856, 1342
CseI GACGC 1 cut(s) 880
Csp6I GTAC 1 cut(s) 682
CviAII CATG 2 cut(s) 547, 580
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 5 cut(s) 236, 428, 1258, 1292, 1338
DpnI GATC 3 cut(s) 572, 911, 933
DpnII GATC 3 cut(s) 570, 909, 931
DraI TTTAAA 1 cut(s) 400
DraIII CACNNNGTG 1 cut(s) 1261
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 536
Eco47I GGWCC 2 cut(s) 856, 1342
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 1043
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 1342
EcoT22I ATGCAT 1 cut(s) 1159
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 2 cut(s) 550, 583
FaqI GGGAC 1 cut(s) 1155
FatI CATG 2 cut(s) 546, 579
FauNDI CATATG 2 cut(s) 248, 1321
FbaI TGATCA 2 cut(s) 570, 909
FblI GTMKAC 2 cut(s) 108, 1109
Fnu4HI GCNGC 1 cut(s) 1269
FokI GGATG 2 cut(s) 632, 1166
Fsp4HI GCNGC 1 cut(s) 1269
FspBI CTAG 1 cut(s) 152
GluI GCNGC 1 cut(s) 1269
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 3 cut(s) 104, 250, 1377
HaeIII GGCC 1 cut(s) 689
HapII CCGG 2 cut(s) 440, 563
HgaI GACGC 1 cut(s) 880
Hin1II CATG 2 cut(s) 550, 583
HindIII AAGCTT 2 cut(s) 300, 1274
HinfI GANTC 5 cut(s) 176, 702, 805, 1032, 1208
HpaII CCGG 2 cut(s) 440, 563
HphI GGTGA 4 cut(s) 107, 518, 544, 595
Hpy166II GTNNAC 5 cut(s) 109, 262, 682, 881, 1110
Hpy188I TCNGA 3 cut(s) 429, 1037, 1213
Hpy188III TCNNGA 5 cut(s) 121, 563, 695, 1345, 1356
Hpy8I GTNNAC 5 cut(s) 109, 262, 682, 881, 1110
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 7 cut(s) 365, 529, 643, 701, 751, 755, 1389
HpyCH4III ACNGT 4 cut(s) 88, 106, 898, 1100
HpyCH4IV ACGT 3 cut(s) 797, 1129, 1144
HpyCH4V TGCA 4 cut(s) 1157, 1264, 1271, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 413, 1246, 1277
HpyF3I CTNAG 5 cut(s) 236, 428, 1258, 1292, 1338
HpySE526I ACGT 3 cut(s) 797, 1129, 1144
Hsp92II CATG 2 cut(s) 550, 583
Kpn2I TCCGGA 1 cut(s) 562
Ksp22I TGATCA 2 cut(s) 570, 909
Kzo9I GATC 3 cut(s) 570, 909, 931
LmnI GCTCC 2 cut(s) 553, 1321
Lsp1109I GCAGC 1 cut(s) 1255
LweI GCATC 4 cut(s) 197, 613, 1062, 1144
MaeI CTAG 1 cut(s) 152
MaeII ACGT 3 cut(s) 797, 1129, 1144
MaeIII GTNAC 5 cut(s) 787, 818, 892, 985, 1368
MalI GATC 3 cut(s) 572, 911, 933
MboI GATC 3 cut(s) 570, 909, 931
MboII GAAGA 4 cut(s) 6, 27, 1191, 1195
MfeI CAATTG 1 cut(s) 1379
MluCI AATT 6 cut(s) 42, 69, 169, 308, 1302, 1379
MlyI GAGTC 2 cut(s) 170, 1041
MmeI TCCRAC 1 cut(s) 597
Mph1103I ATGCAT 1 cut(s) 1159
MroI TCCGGA 1 cut(s) 562
MseI TTAA 7 cut(s) 384, 399, 591, 639, 924, 1121, 1305
MslI CAYNNNNRTG 2 cut(s) 578, 584
MspI CCGG 2 cut(s) 440, 563
MssI GTTTAAAC 1 cut(s) 400
MunI CAATTG 1 cut(s) 1379
Mva1269I GAATGC 1 cut(s) 1196
MvnI CGCG 1 cut(s) 891
MwoI GCNNNNNNNGC 4 cut(s) 365, 413, 1246, 1277
NdeI CATATG 2 cut(s) 248, 1321
NdeII GATC 3 cut(s) 570, 909, 931
NlaIII CATG 2 cut(s) 550, 583
NlaIV GGNNCC 1 cut(s) 29
NmeAIII GCCGAG 1 cut(s) 665
NmuCI GTSAC 2 cut(s) 818, 892
NsiI ATGCAT 1 cut(s) 1159
PctI GAATGC 1 cut(s) 1196
PfeI GAWTC 3 cut(s) 702, 805, 1208
PkrI GCNGC 1 cut(s) 1270
PleI GAGTC 2 cut(s) 170, 1040
PmeI GTTTAAAC 1 cut(s) 400
PpsI GAGTC 2 cut(s) 170, 1040
PpuMI RGGWCCY 1 cut(s) 1342
PshAI GACNNNNGTC 1 cut(s) 825
PsiI TTATAA 2 cut(s) 485, 951
Psp1406I AACGTT 1 cut(s) 1129
Psp5II RGGWCCY 1 cut(s) 1342
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 3 cut(s) 688, 856, 1342
PspPPI RGGWCCY 1 cut(s) 1342
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
RseI CAYNNNNRTG 2 cut(s) 578, 584
SaqAI TTAA 7 cut(s) 384, 399, 591, 639, 924, 1121, 1305
SatI GCNGC 1 cut(s) 1269
Sau3AI GATC 3 cut(s) 570, 909, 931
Sau96I GGNCC 3 cut(s) 688, 856, 1342
SchI GAGTC 2 cut(s) 170, 1041
SfaNI GCATC 4 cut(s) 197, 613, 1062, 1144
SfcI CTRYAG 2 cut(s) 706, 1003
SinI GGWCC 2 cut(s) 856, 1342
SmiMI CAYNNNNRTG 2 cut(s) 578, 584
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
Sse9I AATT 6 cut(s) 42, 69, 169, 308, 1302, 1379
SsiI CCGC 1 cut(s) 521
SspMI CTAG 1 cut(s) 152
TaaI ACNGT 4 cut(s) 88, 106, 898, 1100
TaiI ACGT 3 cut(s) 800, 1132, 1147
TaqI TCGA 1 cut(s) 696
TaqII GACCGA 2 cut(s) 819, 873
TasI AATT 6 cut(s) 42, 69, 169, 308, 1302, 1379
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 3 cut(s) 702, 805, 1208
Tru1I TTAA 7 cut(s) 384, 399, 591, 639, 924, 1121, 1305
Tru9I TTAA 7 cut(s) 384, 399, 591, 639, 924, 1121, 1305
TscAI CASTG 1 cut(s) 840
TseFI GTSAC 2 cut(s) 818, 892
TseI GCWGC 1 cut(s) 1268
Tsp45I GTSAC 2 cut(s) 818, 892
TspDTI ATGAA 5 cut(s) 235, 404, 662, 1188, 1364
TspRI CASTG 1 cut(s) 840
VpaK11BI GGWCC 2 cut(s) 856, 1342
XagI CCTNNNNNAGG 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 108, 1109
XspI CTAG 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.