Rorug07G0082200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008335
Physical Location & Seq
Reverse (-)
6474630 .. 6477179
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0082200.1

Sequence Viewer

Length: 1299 bp
ATGGCTTTGGCCTCGGCTTTGCTGGAGATTCTGCGAAGGCCCACGATTGTTGAAGTGCTTGGGGAGCTTGTGAAGTTCATAACCCCTCTTTGGATTGCTGTGATTGTCGGGGTTTTCGTTGGGTGGGCGTGGCGGCCTAAATGGGTGACCGTGGTTGGTAGAGAATTGTTGGATAGCTCCACGCAAAAGGAGTCGTCTTCCTCATCATCTTCTTCTTCTTCGTCGCCACCCGCACCATCCACGTGTGGGATTTCGATTTCGAGTTTGAGTTCGTTGCTGCCGAATTGCATTCCTTGGGCTGCTGACGATAGAAATCAGAACCAGGCACCTACCACCAATAATGATTGCAGTTCCTCACAGATTGAACAAGAAGAATCCTGTAATGTCTCCAATGAGGATTTACGCCACATATGTCAGCTTGTGGAGGAGAAAGATGGCGGTCCTGCTTGGATTCATATGATGGATCGGTCAACCCCAACTATGGGATATCAAGCTTGGCGTAGAGATCCTCAGACTGGACCTCCACAATACCGAAGTAGAACTGTATTTGAAGATGCTTCACCTGAGACAGTGAGGGATTTCTTTTGGGATGATGAATTTCGTGCAAAGTGGGATGATATGCTTATTCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACCATGCTAGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACACGATAGGCCGTCGCATTTGGGAATCGGGGCGATCATACTACTGTGTAACTAAGGGAGTTCCTTCCTCTGCTGTGCCAAGGCGGGACAAACCTAGACGTGTTGATCTGTACTATTCAAGTTGGTGCATTCGTGCAGTTGAATCAAAAAGAGGGGATGGCCAGCTTACTTCATGTGAGGTTTTACTGTTTCACCATGAAGATATGGGTATTCCATGGGAGATTGCGAAACTTGGTGTTCGTCAAGGTATGTGGGGAGCTGTTAAAAAGATTGAACCAGGCCTACGTTTATATCAGAAGGAAAGAGCCGCTAAAGTGCCACTTTCGCGGTGTGCTAAAATGGCTCAGATCAACACGAAAGTCAGTGCTGATTACTTGAGATCGGTAGAAAACAGCACTAAGGATTCCATGGAGGTTGAAAATTCAGATCCAGCGGAGAAACCAGCAAGGAGGAACATACCTAAAGCTCTAGTTGTCGGAGGGGCAATAGTTCTTGCTTGCAGTCTTGATCGGGGCCTCTTGACTAAGGCAGTTATATTTGGAGTAGCTCGAAGATTTGCCAAGATCGGGAGGAGGTTGTGA

Protein Analysis

432

Amino Acids

48.51

Weight (kDa)

8.65

Isoelectric Point (pI)

58.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 149 - 304 2.2e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AccII CGCG 1 cut(s) 1045
AciI CCGC 8 cut(s) 133, 231, 438, 685, 802, 1026, 1045, 1151
AclWI GGATC 3 cut(s) 471, 500, 1139
AcoI YGGCCR 1 cut(s) 877
AcsI RAATTY 2 cut(s) 596, 1138
AcvI CACGTG 1 cut(s) 243
AfaI GTAC 1 cut(s) 830
AfiI CCNNNNNNNGG 7 cut(s) 90, 246, 481, 482, 515, 659, 1284
AflIII ACRYGT 2 cut(s) 242, 817
AgsI TTSAA 7 cut(s) 53, 365, 551, 837, 860, 992, 1136
AjiI CACGTC 1 cut(s) 818
AjnI CCWGG 2 cut(s) 321, 994
AluBI AGCT 8 cut(s) 67, 177, 418, 494, 883, 977, 1184, 1265
AluI AGCT 8 cut(s) 67, 177, 418, 494, 883, 977, 1184, 1265
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 2 cut(s) 391, 560
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 3 cut(s) 471, 500, 1139
AoxI GGCC 7 cut(s) 9, 38, 134, 727, 877, 997, 1231
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 2 cut(s) 277, 299
ApoI RAATTY 2 cut(s) 596, 1138
AspS9I GGNCC 5 cut(s) 39, 440, 518, 682, 1231
AsuHPI GGTGA 3 cut(s) 157, 552, 902
AvaII GGWCC 3 cut(s) 440, 518, 682
BaeGI GKGCMC 1 cut(s) 678
BalI TGGCCA 1 cut(s) 879
BanI GGYRCC 1 cut(s) 325
BbrPI CACGTG 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 264, 286
BccI CCATC 4 cut(s) 244, 428, 454, 869
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 2 cut(s) 323, 996
BcoDI GTCTC 2 cut(s) 391, 560
BfaI CTAG 3 cut(s) 671, 813, 1187
BisI GCNGC 4 cut(s) 134, 278, 300, 1026
BlsI GCNGC 4 cut(s) 135, 279, 301, 1027
Bme1390I CCNGG 2 cut(s) 323, 996
Bme18I GGWCC 3 cut(s) 440, 518, 682
BmgBI CACGTC 1 cut(s) 818
BmgT120I GGNCC 5 cut(s) 39, 440, 518, 682, 1231
BmiI GGNNCC 4 cut(s) 327, 664, 683, 1232
BmrFI CCNGG 2 cut(s) 323, 996
BmrI ACTGGG 1 cut(s) 688
BmsI GCATC 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 688
BpiI GAAGAC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 1114
BsaAI YACGTR 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 6 cut(s) 12, 150, 293, 797, 932, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 246, 481, 482, 515, 659, 1284
Bse1I ACTGG 3 cut(s) 520, 664, 683
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 2 cut(s) 323, 996
BseDI CCNNGG 6 cut(s) 12, 150, 293, 797, 932, 1125
BseGI GGATG 4 cut(s) 236, 595, 619, 880
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 7 cut(s) 90, 246, 481, 482, 515, 659, 1284
BseMII CTCAG 3 cut(s) 524, 555, 1076
BseNI ACTGG 3 cut(s) 520, 664, 683
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 2 cut(s) 264, 286
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 1 cut(s) 1045
BshFI GGCC 7 cut(s) 11, 40, 136, 729, 879, 999, 1233
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 678
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 7 cut(s) 90, 246, 481, 482, 515, 659, 1284
BsmAI GTCTC 2 cut(s) 391, 560
BsmFI GGGAC 1 cut(s) 818
BsmI GAATGC 3 cut(s) 288, 653, 846
BsnI GGCC 7 cut(s) 11, 40, 136, 729, 879, 999, 1233
Bsp1286I GDGCHC 1 cut(s) 678
Bsp143I GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
Bsp19I CCATGG 2 cut(s) 932, 1125
BspACI CCGC 8 cut(s) 133, 231, 438, 685, 802, 1026, 1045, 1151
BspANI GGCC 7 cut(s) 11, 40, 136, 729, 879, 999, 1233
BspCNI CTCAG 3 cut(s) 523, 556, 1075
BspFNI CGCG 1 cut(s) 1045
BspLI GGNNCC 4 cut(s) 327, 664, 683, 1232
BspPI GGATC 3 cut(s) 471, 500, 1139
BspT107I GGYRCC 1 cut(s) 325
BsrI ACTGG 3 cut(s) 520, 664, 683
BssECI CCNNGG 6 cut(s) 12, 150, 293, 797, 932, 1125
BssMI GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
BssT1I CCWWGG 4 cut(s) 293, 797, 932, 1125
Bst2UI CCWGG 2 cut(s) 323, 996
Bst4CI ACNGT 5 cut(s) 151, 544, 571, 764, 906
BstBAI YACGTR 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 881, 1216
BstDEI CTNAG 6 cut(s) 510, 564, 771, 1062, 1116, 1242
BstDSI CCRYGG 3 cut(s) 150, 932, 1125
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 4 cut(s) 236, 595, 619, 880
BstFNI CGCG 1 cut(s) 1045
BstKTI GATC 9 cut(s) 466, 508, 755, 826, 1068, 1100, 1147, 1228, 1284
BstMAI GTCTC 2 cut(s) 391, 560
BstMBI GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
BstMWI GCNNNNNNNGC 3 cut(s) 64, 1042, 1058
BstNI CCWGG 2 cut(s) 323, 996
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 321, 994
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 1 cut(s) 1045
BstV1I GCAGC 2 cut(s) 264, 286
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 505, 1144
BstYI RGATCY 2 cut(s) 505, 1144
BsuRI GGCC 7 cut(s) 11, 40, 136, 729, 879, 999, 1233
BtgI CCRYGG 3 cut(s) 150, 932, 1125
BtrI CACGTC 1 cut(s) 818
BtsCI GGATG 4 cut(s) 236, 595, 619, 880
BtsIMutI CAGTG 3 cut(s) 576, 676, 1087
Cac8I GCNNGC 2 cut(s) 881, 1216
Cfr13I GGNCC 5 cut(s) 39, 440, 518, 682, 1231
Csp6I GTAC 1 cut(s) 829
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 950, 985
CviAII CATG 6 cut(s) 629, 667, 891, 914, 933, 1126
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 6 cut(s) 510, 564, 771, 1062, 1116, 1242
DpnI GATC 9 cut(s) 465, 507, 754, 825, 1067, 1099, 1146, 1227, 1283
DpnII GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
EaeI YGGCCR 1 cut(s) 877
Eco130I CCWWGG 4 cut(s) 293, 797, 932, 1125
Eco147I AGGCCT 1 cut(s) 999
Eco32I GATATC 1 cut(s) 488
Eco47I GGWCC 3 cut(s) 440, 518, 682
Eco72I CACGTG 1 cut(s) 243
Eco91I GGTNACC 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 1231
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 321, 994
EcoRV GATATC 1 cut(s) 488
EcoT14I CCWWGG 4 cut(s) 293, 797, 932, 1125
ErhI CCWWGG 4 cut(s) 293, 797, 932, 1125
FaeI CATG 6 cut(s) 632, 670, 894, 917, 936, 1129
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 1 cut(s) 818
FatI CATG 6 cut(s) 628, 666, 890, 913, 932, 1125
FauI CCCGC 2 cut(s) 238, 795
FauNDI CATATG 2 cut(s) 410, 456
Fnu4HI GCNGC 4 cut(s) 134, 278, 300, 1026
FokI GGATG 4 cut(s) 223, 602, 626, 887
Fsp4HI GCNGC 4 cut(s) 134, 278, 300, 1026
FspBI CTAG 3 cut(s) 671, 813, 1187
GluI GCNGC 4 cut(s) 134, 278, 300, 1026
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 7 cut(s) 11, 40, 136, 729, 879, 999, 1233
Hin1II CATG 6 cut(s) 632, 670, 894, 917, 936, 1129
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 7 cut(s) 28, 191, 374, 451, 743, 860, 1121
HphI GGTGA 3 cut(s) 157, 552, 902
Hpy166II GTNNAC 2 cut(s) 471, 676
Hpy188I TCNGA 6 cut(s) 318, 513, 1014, 1065, 1144, 1196
Hpy188III TCNNGA 3 cut(s) 1223, 1237, 1285
Hpy8I GTNNAC 2 cut(s) 471, 676
Hpy99I CGWCG 2 cut(s) 226, 735
HpyAV CCTTC 3 cut(s) 30, 792, 1009
HpyCH4III ACNGT 5 cut(s) 151, 544, 571, 764, 906
HpyCH4IV ACGT 3 cut(s) 242, 817, 1003
HpyCH4V TGCA 7 cut(s) 288, 348, 605, 676, 846, 854, 1218
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 1042, 1058
HpyF3I CTNAG 6 cut(s) 510, 564, 771, 1062, 1116, 1242
HpySE526I ACGT 3 cut(s) 242, 817, 1003
Hsp92II CATG 6 cut(s) 632, 670, 894, 917, 936, 1129
Kzo9I GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
LmnI GCTCC 3 cut(s) 64, 182, 974
Lsp1109I GCAGC 2 cut(s) 264, 286
LweI GCATC 1 cut(s) 544
MaeI CTAG 3 cut(s) 671, 813, 1187
MaeII ACGT 3 cut(s) 242, 817, 1003
MaeIII GTNAC 2 cut(s) 145, 766
MalI GATC 9 cut(s) 465, 507, 754, 825, 1067, 1099, 1146, 1227, 1283
MboI GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
MflI RGATCY 2 cut(s) 505, 1144
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 879
MluCI AATT 4 cut(s) 164, 283, 596, 1138
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 150, 1174
Mox20I TGGCCA 1 cut(s) 879
MscI TGGCCA 1 cut(s) 879
MseI TTAA 1 cut(s) 981
MslI CAYNNNNRTG 2 cut(s) 241, 671
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 1151
MspR9I CCNGG 2 cut(s) 323, 996
Mva1269I GAATGC 3 cut(s) 288, 653, 846
MvaI CCWGG 2 cut(s) 323, 996
MvnI CGCG 1 cut(s) 1045
MwoI GCNNNNNNNGC 3 cut(s) 64, 1042, 1058
NcoI CCATGG 2 cut(s) 932, 1125
NdeI CATATG 2 cut(s) 410, 456
NdeII GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
NlaIII CATG 6 cut(s) 632, 670, 894, 917, 936, 1129
NlaIV GGNNCC 4 cut(s) 327, 664, 683, 1232
NmuCI GTSAC 1 cut(s) 145
PceI AGGCCT 1 cut(s) 999
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 278
PctI GAATGC 3 cut(s) 288, 653, 846
PfeI GAWTC 6 cut(s) 28, 374, 451, 743, 860, 1121
PkrI GCNGC 4 cut(s) 135, 279, 301, 1027
PleI GAGTC 1 cut(s) 199
PmaCI CACGTG 1 cut(s) 243
PmlI CACGTG 1 cut(s) 243
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 321, 994
PspCI CACGTG 1 cut(s) 243
PspEI GGTNACC 1 cut(s) 145
PspGI CCWGG 2 cut(s) 321, 994
PspN4I GGNNCC 4 cut(s) 327, 664, 683, 1232
PspPI GGNCC 5 cut(s) 39, 440, 518, 682, 1231
PsrI GAACNNNNNNTAC 2 cut(s) 984, 1016
PsuI RGATCY 2 cut(s) 505, 1144
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
RseI CAYNNNNRTG 2 cut(s) 241, 671
SaqAI TTAA 1 cut(s) 981
SatI GCNGC 4 cut(s) 134, 278, 300, 1026
Sau3AI GATC 9 cut(s) 463, 505, 752, 823, 1065, 1097, 1144, 1225, 1281
Sau96I GGNCC 5 cut(s) 39, 440, 518, 682, 1231
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 323, 996
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 1 cut(s) 544
SinI GGWCC 3 cut(s) 440, 518, 682
SmiMI CAYNNNNRTG 2 cut(s) 241, 671
SmlI CTYRAG 1 cut(s) 1093
SmoI CTYRAG 1 cut(s) 1093
Sse9I AATT 4 cut(s) 164, 283, 596, 1138
SseBI AGGCCT 1 cut(s) 999
SsiI CCGC 8 cut(s) 133, 231, 438, 685, 802, 1026, 1045, 1151
SspMI CTAG 3 cut(s) 671, 813, 1187
StuI AGGCCT 1 cut(s) 999
StyD4I CCNGG 2 cut(s) 321, 994
StyI CCWWGG 4 cut(s) 293, 797, 932, 1125
TaaI ACNGT 5 cut(s) 151, 544, 571, 764, 906
TaiI ACGT 3 cut(s) 245, 820, 1006
TaqI TCGA 3 cut(s) 254, 260, 1267
TaqII GACCGA 1 cut(s) 456
TasI AATT 4 cut(s) 164, 283, 596, 1138
TatI WGTACW 1 cut(s) 828
TauI GCSGC 2 cut(s) 136, 1028
TfiI GAWTC 6 cut(s) 28, 374, 451, 743, 860, 1121
Tru1I TTAA 1 cut(s) 981
Tru9I TTAA 1 cut(s) 981
TscAI CASTG 3 cut(s) 576, 683, 1087
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 277, 299
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 6 cut(s) 67, 443, 609, 617, 879, 930
TspRI CASTG 3 cut(s) 576, 683, 1087
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 440, 518, 682
XapI RAATTY 2 cut(s) 596, 1138
XspI CTAG 3 cut(s) 671, 813, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.