RchiOBHm_Chr7g0204361

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008335
Physical Location & Seq
Forward (+)
22041262 .. 22047615
6354 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18291

Sequence Viewer

Length: 1560 bp
ATGAAGCTTAACTCGATCCGACCCGATACCCGTTTCAAGCCCAATTCTCTTCCATTTCGGCCGGAAAGTCTGTGTCTCCTCCATTTCAGCGTCAAAGGGTTTAACCAAAGCCCTAAAACTTTCCTTGAAATGACGACCCTTTCTTCAATCTCTCGGTTCCTCTCTTCCTCAATTCGCCTCAAAAATCACAAGCTCTCAATTTCTCACTTCTCTACTGTCTCGTCGGCAGAGACGGTCTACAATCACCTCCAGAGAAATGGTGGCAACATTGAGAAAACCCTAGCCTCAATGAGGCTCAATTTGGACGCCAAATGTGTAAGTCAGGTTTTACATAGATGCTATCCCACCCAATCCCAAATGGGTCTTAGATTTTTCATATGGGCTGGGGTTCACTCCAGTTATAGGCATAGCTATTTTATGTTCAGCAAAGCTTGTGAATTGTATAGGATTAGGCAAAACCCAAGTGTGATTTTTGATGTTTTGGAGGCTTATAGTGTTGAAGGTTGCTCTGTTAATATGAAAATGTTTAAAGTTGTTTTCAATATGTGTAAAGAAGCTAAGCTTGCTGATGAGGCTTTGGGGGTTCTGAGGAAAATGCCGGAGTTCGGGTTGCGAGGGGATAATGTAGTGTATAATGTTGTTATAAGGCTGTTTTGTGATAAGGGTGATTTGGATATGGCTGAGAGTTTGGTGAAGGAGATGAGCATGGTGGAGCTTTATCCGGATTTGATCACATACATGGTGATGATTAAAGGGTTGTGTAATGTGGGTCGGTTGGATGATGCGTGTGGGTTGTTTAAGTTTATGAAGGAAAATGGTTGTTTGCCTAATGTTGTGGTGTTCTCGGCCCTTCTTGATGGATTCTGTAGGTTTGGGGATATGGAGAGAGCCTTGAAGTTGTTAGAGGAGATGGAGAAGGAAGGTGGGGATTGTAGTCCTAATGTAGTAACATATACGTCTGTGATTCAATATCTTTGTGACAAGGGTCGGACAGTGGAGGCATTGCTTGTTTTGGATCGAATGGAAGCTCGTGGTTGTTTACCAACTCGCGTCACGGTTAGTTCTTTGATCACGGGTTTTGTTAAGGAGGATCAAGTTGAGCTGGCTTATAAGTTGGTTGATAGAGTTGTTGAAAGTGGAAGTGTTACAAAAACTGATTGCTATAGTTCTCTTGTTGTGTCTTTGGAAAGAGTCAGAAAACCCGAGGAGGCAGAGAAAGTCTTTAGGACGATGCTTGATAGTGGGGTCAAACCAAACAGTTTGGTGTGTACCATTATGTTAAAAAAAAGTTGTTTGGAGGGACGTATGGTGGATGCATATTGCTTGTTTGATGAACTTGAGAAGATGGAATGCTTATCTTCTATTGATTCTGATACTTATTCTATTCTTTTAGTGGGGCTTTGTCAGCAAAGACACTTAGTGCAGGCTGCAAAGCTTGCAAGGGTAATGCTGAGTAAAGGAATTAAACTAAAAGCTCCATATGTTGATATTATAGCTGAGGTCCTGATGAAATCTGGAGATGAGGAGTTACTTGAACAATTGACAAGAAATGGAAGGTGA

Protein Analysis

519

Amino Acids

58.61

Weight (kDa)

8.27

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 160 - 263 1.3e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 207 - 255 3.4e-12 PPR repeat family
PPR_3 PF13812 229 - 286 2.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 239 - 270 1.9e-08 PPR repeat
PPR_2 PF13041 241 - 290 3.6e-15 PPR repeat family
PPR PF01535 244 - 274 3.7e-07 PPR repeat
PPR_long PF17177 261 - 379 9.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 272 - 305 3.4e-12 PPR repeat
PPR_2 PF13041 276 - 308 5.4e-08 PPR repeat family
PPR_3 PF13812 277 - 324 3.9e-07 Pentatricopeptide repeat domain
PPR PF01535 279 - 308 1.2e-07 PPR repeat
PPR_3 PF13812 299 - 362 3.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 310 - 342 6.7e-09 PPR repeat
PPR_2 PF13041 313 - 362 1.8e-13 PPR repeat family
PPR_2 PF13041 387 - 431 7.2e-06 PPR repeat family
PPR_2 PF13041 423 - 469 5.6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 644, 1110
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 1050
AccIII TCCGGA 1 cut(s) 721
AclWI GGATC 3 cut(s) 10, 1023, 1098
AcoI YGGCCR 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 306
AdeI CACNNNGTG 1 cut(s) 1420
AfaI GTAC 1 cut(s) 1270
AfiI CCNNNNNNNGG 3 cut(s) 291, 402, 605
AgsI TTSAA 9 cut(s) 37, 128, 147, 500, 541, 895, 968, 1133, 1535
Alw26I GTCTC 3 cut(s) 80, 223, 224
AlwI GGATC 3 cut(s) 10, 1023, 1098
Ama87I CYCGRG 1 cut(s) 1202
Aor13HI TCCGGA 1 cut(s) 721
AoxI GGCC 2 cut(s) 59, 846
ApeKI GCWGC 1 cut(s) 1427
AspS9I GGNCC 2 cut(s) 847, 1501
AsuHPI GGTGA 4 cut(s) 236, 677, 703, 754
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 1 cut(s) 1501
BauI CACGAG 1 cut(s) 1029
BbvCI CCTCAGC 1 cut(s) 1497
BbvI GCAGC 1 cut(s) 1414
BccI CCATC 3 cut(s) 851, 904, 1339
BclI TGATCA 2 cut(s) 729, 1068
BcoDI GTCTC 3 cut(s) 80, 223, 224
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 865, 1162
BisI GCNGC 1 cut(s) 1428
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 1429
Bme18I GGWCC 1 cut(s) 1501
BmeT110I CYCGRG 1 cut(s) 1202
BmgT120I GGNCC 2 cut(s) 847, 1501
BmiI GGNNCC 1 cut(s) 158
BmsI GCATC 4 cut(s) 326, 772, 1221, 1303
BoxI GACNNNNGTC 1 cut(s) 984
BpmI CTGGAG 3 cut(s) 233, 379, 1536
Bpu10I CCTNAGC 1 cut(s) 1497
Bpu1102I GCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 1358
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 1203
BsaWI WCCGGW 1 cut(s) 721
BsaXI ACNNNNNCTCC 2 cut(s) 593, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 402, 605
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 1001
BseAI TCCGGA 1 cut(s) 721
BseDI CCNNGG 1 cut(s) 1203
BseGI GGATG 2 cut(s) 784, 1318
BseLI CCNNNNNNNGG 3 cut(s) 291, 402, 605
BseMI GCAATG 1 cut(s) 1001
BseMII CTCAG 4 cut(s) 578, 672, 1442, 1488
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 4 cut(s) 68, 920, 1220, 1538
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 1414
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 1442
Bsh1236I CGCG 1 cut(s) 1050
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 61, 848
BsiEI CGRYCG 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 3 cut(s) 62, 599, 722
BslFI GGGAC 1 cut(s) 1314
BslI CCNNNNNNNGG 3 cut(s) 291, 402, 605
BsmAI GTCTC 3 cut(s) 80, 223, 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 1314
BsmI GAATGC 1 cut(s) 1355
BsnI GGCC 2 cut(s) 61, 848
BsoBI CYCGRG 1 cut(s) 1202
Bsp13I TCCGGA 1 cut(s) 721
Bsp143I GATC 5 cut(s) 15, 729, 1015, 1068, 1090
Bsp1720I GCTNAGC 1 cut(s) 558
BspANI GGCC 2 cut(s) 61, 848
BspCNI CTCAG 4 cut(s) 579, 673, 1443, 1489
BspEI TCCGGA 1 cut(s) 721
BspFNI CGCG 1 cut(s) 1050
BspLI GGNNCC 1 cut(s) 158
BspPI GGATC 3 cut(s) 10, 1023, 1098
BsrDI GCAATG 1 cut(s) 1001
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 1 cut(s) 1203
BssMI GATC 5 cut(s) 15, 729, 1015, 1068, 1090
BssNI GRCGYC 1 cut(s) 306
BssSI CACGAG 1 cut(s) 1029
Bst2BI CACGAG 1 cut(s) 1029
Bst4CI ACNGT 5 cut(s) 217, 235, 994, 1057, 1259
Bst6I CTCTTC 2 cut(s) 54, 169
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 1436
BstC8I GCNNGC 4 cut(s) 564, 1104, 1425, 1437
BstDEI CTNAG 7 cut(s) 365, 558, 587, 681, 1417, 1451, 1497
BstENI CCTNNNNNAGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 784, 1318
BstFNI CGCG 1 cut(s) 1050
BstKTI GATC 5 cut(s) 18, 732, 1018, 1071, 1093
BstMAI GTCTC 3 cut(s) 80, 223, 224
BstMBI GATC 5 cut(s) 15, 729, 1015, 1068, 1090
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 4 cut(s) 563, 572, 1405, 1436
BstPAI GACNNNNGTC 1 cut(s) 984
BstSFI CTRYAG 2 cut(s) 865, 1162
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 1 cut(s) 1414
BstXI CCANNNNNNTGG 1 cut(s) 257
BstZI CGGCCG 1 cut(s) 59
BsuRI GGCC 2 cut(s) 61, 848
BtsCI GGATG 2 cut(s) 784, 1318
BtsIMutI CAGTG 1 cut(s) 999
Cac8I GCNNGC 4 cut(s) 564, 1104, 1425, 1437
Cfr13I GGNCC 2 cut(s) 847, 1501
CseI GACGC 3 cut(s) 79, 314, 1039
Csp6I GTAC 1 cut(s) 1269
CviAII CATG 2 cut(s) 706, 739
CviQI GTAC 1 cut(s) 1269
DdeI CTNAG 7 cut(s) 365, 558, 587, 681, 1417, 1451, 1497
DpnI GATC 5 cut(s) 17, 731, 1017, 1070, 1092
DpnII GATC 5 cut(s) 15, 729, 1015, 1068, 1090
DraI TTTAAA 1 cut(s) 529
DraIII CACNNNGTG 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 59
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 2 cut(s) 54, 169
EarI CTCTTC 2 cut(s) 54, 169
EclXI CGGCCG 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 1501
Eco52I CGGCCG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 1202
EcoNI CCTNNNNNAGG 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 1501
EcoT22I ATGCAT 1 cut(s) 1318
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 2 cut(s) 709, 742
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 1 cut(s) 1314
FatI CATG 2 cut(s) 705, 738
FauNDI CATATG 2 cut(s) 377, 1480
FbaI TGATCA 2 cut(s) 729, 1068
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 1428
FokI GGATG 2 cut(s) 791, 1325
Fsp4HI GCNGC 1 cut(s) 1428
FspBI CTAG 1 cut(s) 281
GluI GCNGC 1 cut(s) 1428
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 3 cut(s) 233, 379, 1536
HaeIII GGCC 2 cut(s) 61, 848
HapII CCGG 3 cut(s) 62, 599, 722
HgaI GACGC 3 cut(s) 79, 314, 1039
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 2 cut(s) 709, 742
HindIII AAGCTT 4 cut(s) 5, 429, 560, 1433
HinfI GANTC 4 cut(s) 861, 964, 1191, 1367
HpaII CCGG 3 cut(s) 62, 599, 722
HphI GGTGA 4 cut(s) 236, 677, 703, 754
Hpy166II GTNNAC 4 cut(s) 238, 391, 1040, 1269
Hpy188I TCNGA 5 cut(s) 20, 588, 990, 1196, 1372
Hpy188III TCNNGA 5 cut(s) 250, 722, 854, 1504, 1515
Hpy8I GTNNAC 4 cut(s) 238, 391, 1040, 1269
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 7 cut(s) 494, 688, 802, 860, 910, 914, 1548
HpyCH4III ACNGT 5 cut(s) 217, 235, 994, 1057, 1259
HpyCH4IV ACGT 2 cut(s) 956, 1303
HpyCH4V TGCA 4 cut(s) 1316, 1423, 1430, 1439
HpyF10VI GCNNNNNNNGC 4 cut(s) 563, 572, 1405, 1436
HpyF3I CTNAG 7 cut(s) 365, 558, 587, 681, 1417, 1451, 1497
HpySE526I ACGT 2 cut(s) 956, 1303
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 2 cut(s) 709, 742
Kpn2I TCCGGA 1 cut(s) 721
Ksp22I TGATCA 2 cut(s) 729, 1068
Kzo9I GATC 5 cut(s) 15, 729, 1015, 1068, 1090
LmnI GCTCC 2 cut(s) 712, 1480
Lsp1109I GCAGC 1 cut(s) 1414
LweI GCATC 4 cut(s) 326, 772, 1221, 1303
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 956, 1303
MaeIII GTNAC 5 cut(s) 946, 977, 1051, 1144, 1527
MalI GATC 5 cut(s) 17, 731, 1017, 1070, 1092
MboI GATC 5 cut(s) 15, 729, 1015, 1068, 1090
MboII GAAGA 5 cut(s) 41, 135, 156, 1350, 1354
MfeI CAATTG 1 cut(s) 1538
MluCI AATT 7 cut(s) 43, 171, 198, 298, 437, 1461, 1538
MlyI GAGTC 1 cut(s) 1200
MmeI TCCRAC 3 cut(s) 43, 756, 968
Mph1103I ATGCAT 1 cut(s) 1318
MroI TCCGGA 1 cut(s) 721
MseI TTAA 9 cut(s) 9, 102, 513, 528, 750, 798, 1083, 1280, 1464
MslI CAYNNNNRTG 2 cut(s) 737, 743
MspI CCGG 3 cut(s) 62, 599, 722
MunI CAATTG 1 cut(s) 1538
Mva1269I GAATGC 1 cut(s) 1355
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 4 cut(s) 563, 572, 1405, 1436
NdeI CATATG 2 cut(s) 377, 1480
NdeII GATC 5 cut(s) 15, 729, 1015, 1068, 1090
NlaIII CATG 2 cut(s) 709, 742
NlaIV GGNNCC 1 cut(s) 158
NmeAIII GCCGAG 1 cut(s) 824
NmuCI GTSAC 2 cut(s) 977, 1051
NsiI ATGCAT 1 cut(s) 1318
PctI GAATGC 1 cut(s) 1355
PfeI GAWTC 3 cut(s) 861, 964, 1367
PkrI GCNGC 1 cut(s) 1429
PleI GAGTC 1 cut(s) 1199
PpsI GAGTC 1 cut(s) 1199
PpuMI RGGWCCY 1 cut(s) 1501
PshAI GACNNNNGTC 1 cut(s) 984
PsiI TTATAA 2 cut(s) 644, 1110
Psp5II RGGWCCY 1 cut(s) 1501
PspFI CCCAGC 1 cut(s) 383
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 2 cut(s) 847, 1501
PspPPI RGGWCCY 1 cut(s) 1501
RsaI GTAC 1 cut(s) 1270
RsaNI GTAC 1 cut(s) 1269
RseI CAYNNNNRTG 2 cut(s) 737, 743
SaqAI TTAA 9 cut(s) 9, 102, 513, 528, 750, 798, 1083, 1280, 1464
SatI GCNGC 1 cut(s) 1428
Sau3AI GATC 5 cut(s) 15, 729, 1015, 1068, 1090
Sau96I GGNCC 2 cut(s) 847, 1501
SchI GAGTC 1 cut(s) 1200
SfaNI GCATC 4 cut(s) 326, 772, 1221, 1303
SfcI CTRYAG 2 cut(s) 865, 1162
SinI GGWCC 1 cut(s) 1501
SmiMI CAYNNNNRTG 2 cut(s) 737, 743
SmlI CTYRAG 1 cut(s) 1337
SmoI CTYRAG 1 cut(s) 1337
Sse9I AATT 7 cut(s) 43, 171, 198, 298, 437, 1461, 1538
SspMI CTAG 1 cut(s) 281
TaaI ACNGT 5 cut(s) 217, 235, 994, 1057, 1259
TaiI ACGT 2 cut(s) 959, 1306
TaqI TCGA 2 cut(s) 14, 1018
TasI AATT 7 cut(s) 43, 171, 198, 298, 437, 1461, 1538
TfiI GAWTC 3 cut(s) 861, 964, 1367
Tru1I TTAA 9 cut(s) 9, 102, 513, 528, 750, 798, 1083, 1280, 1464
Tru9I TTAA 9 cut(s) 9, 102, 513, 528, 750, 798, 1083, 1280, 1464
TscAI CASTG 1 cut(s) 999
TseFI GTSAC 2 cut(s) 977, 1051
TseI GCWGC 1 cut(s) 1427
Tsp45I GTSAC 2 cut(s) 977, 1051
TspDTI ATGAA 6 cut(s) 17, 364, 533, 821, 1347, 1523
TspRI CASTG 1 cut(s) 999
VpaK11BI GGWCC 1 cut(s) 1501
XagI CCTNNNNNAGG 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.