Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006211

Physical Location & Seq

Genomic Coordinates Forward (+)

20176080 .. 20188567

Gene Structure 12488 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G49680.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7764 bp

ATGGCAGCTTTCCTCGTCATGTTTATCTTCACGATCGCTCTGTTCGTCGCACTCCTTTGGGTTTTCTTCAAGTCTCTCCCTTGGATTCTCCGGCATAGTGCTGGAATAACTCTCAGTTTTCAGTTTGATGGATGGAACTGCTTGAAGGATGTTGCATTGCAATTCAAGAAGGGTTCTATTGAATCTATAGTCATTGGAGAATTTAAAGCCAATCTAAGTCAATCCCTGGTTGAACTTTGTGCTACGGCTTTTATCCAGGACCCTAAAGTTATATTCTCTATATGTGACCTGAAAATAGTTACTAGACCCTCCCACTCAAGTAAAGGTCCGCGCAAACCAAAAACTCGGAAATCGAGCTCTGGTGGCAAGGGAAAGTTGATGCTATTTGCTAATATTGGCAGATTCTTTTCAGTTTCCATGACAAATATGGTAGTGCAGACACCTAAAGCTACAGCAGAAATCAAGGAGTTGGAGCTCGATTTATCTAAGGACAGAGGGTCAGGGAATTTTTTCATGAAGTTGTATCTATTGCCGATATTTGTACAGATTGGTGAACCACATGTTACTTCCACTCATTCACCTGAGATGGACAGTGACATTTGCCTCGCTAGGCAGACACCTTCCAAAACCGCAGAGGGGTCTTCTTCTTCTTCTTTTCATTGCGAAAAGATCTCCTTGTCCTGCGAATTTGGTCCTAATAGAAAATCAAGTCCAAGTATAAAGAACGTCGAAGTAGACCTAGCTAATGCTGTTTTAAACCTTAACGAGAAGCTGCTGTTGAAAAACAAATCCTCGACAAGTGCTGCTTCCAAAGGTGAAGTGATTGATTCAAGTTCAGGTAACACCACTTCAGAGAAGCCTCCAAAGCAACCAATGAATGTTTTGGTTGCAAAGCATGCTTCAAAATTCCCTGAGAAGGTTTTATTTGATTTAACGAAGCTTGAAATCAGATTTGTGCATCAAGAACATGATTTTTCTATCGCCAACAGCATAAAAGGCTTTCAATTGAGAAGTGCCAAATCTCAATCTGGTGAAGATGGCAAGGAAGACACATGTCTTGATTTTGCCATGGAACTCCAGGAGATGCATCTTTTTAGAGAATCTGAAGTTTCTGTCTTGGAGATGACAAAATTTGGAGTTTTCACCAAGGTGTATTGTCCGATACAGGAATCCTTACCTGTTAGAGCCGAGGTTGAGATTAAGCTGGGAGGTATAATGAGCAACATTATAATGACGAGATTTGAGCCGCTGTTGCGTTTGCACTTCTCTAGAAAGAAAAAGATGGTTCTCAAAGAGGAAAGACCTACCATTGCAAAATCAGAAACCACTGGTTTTAAGGCTGTGGTATGGAAATGTGCCACCTCAGCCCCTGATGTGACGGTTGTGCTATACAATCCTGGCGGTTCTCCTATATACCAATGCGGCTTAGATTCATTTCAGGCCACTGCAAATAATATGTCAAATAGGGGTACAGTTGTACAGATGGAGCTAAATGAACTGACTTTATGCATGGTAGATGAACATAAGGGATGTTTGAAAGAAAGCCTTTTTGGTTTAGAGTCAGATCCAGGTTCATTAATCAATATAAGAAAGGTCAGATCGGAATGGGGCAAGAAAGAAGTGTTACCTGAGGGCGATGGTTCTAAGGGTAAACAAACTCTGGTGGTTGACGTATCAGAAATAGGTCTTTTATTTTCTTTCAGGAGTGTTGAAGCGCTTACAGTAAATGCAATATCAAGTCAGGCTTATATTAAAAGTTTAACTGGTTCTAGTAGTAAGAACAAACAGGAAAAAGGGGCACATAGATCGAAGCCGCCATCGGGCAGAGGGACTCAACTGTTGAAACTGAATGTTGAACGTTTCTCTCTTAATTTCGCTGGAGATTCAAGCCTGGAAAATACAGTCATTGATGATCCTAAACGTGTCAATTATGGATCACAGGGGGGAAGAATTATAATTAGTGTCTCAGCTGACGGCTCACCTCGAACTGCCAGCGTTTTCTCTACTTTGTCCGAAGAACATGAGAAGTTGAAGTACATAATTTCGTTTGAAATACTCAAATTCGGATTTACTCTAAACAAGGAGATTCAGTCTACACAGGTAGAACTTGAAACAGCAAAGGCGATCTACCAGGAATTTTTGGAGGAACCTCACCAAGTTTCCAGAGTGACACTTTGTGACATCCAAAACGCTAAGTTTGTACGTCGAATAGGCGGTGGTAAGGAAGTTTCAATCTGTTCGCTCTTCAGTGCCTCTAATATTGCCGTCAGATGGGAGCCTGATGTGCACATATCAATGGTCGAGCTTGGTCTGCGTTTGAAGTCCCTAGTCTTAACTCAGAAACTTAAGCAACATGGTAACAGAAACCCTGAAGAGGCTTCCACTGTAACCGGTGATAAACAGAAGGAAGAACCAACTACTACCCCGAATTCACTTGATAAGAAAAAGAAGAAGGAATCTATATTTGCTGTTGACGTAGAGATGTTAAGCATAACTGCGGAGGCTGGGGATGGGGTGGAGGCTGAGGTGCAGATTCAGTCAATTTTCTCAGAGAATGTGGGTATTGGAGTACTTCTGGAAGGATTTATGCTTGGTTTTTGTGGATGTAGAATAGTCAAAAGTAGCCGGGTACAGATATCACGAATACCCAGCATGCCTTCCACTTCATCCAGTGTGACCCCAGCAACAGGTGGGACTCCATGGGATTGGATAGTTCAAGGAGTTGATATACATATATGCATGCCCTTCAGGCTACAGCTCCGTGCTATTGATGATGCTGTTGAAGAAATGCTGAGGGCTTTGAAGCTTGTAACCAACGCCAAAACCAAACTCATTTTTCCAATAAAAAAGGAAAGCTCAACACCTAAAAAACCTGGGTCGAAAAAAGTTGGGCGTATAAGATTCGGCATTCGCAAGTTAATATTTGATATTGAGGAAGAACCCCTCCAGGGTTGGCTTGATGAACATTACCATCTTATGAGGAAAGAAGCTTATGAGTTAGCCATAAGGTCGAAATTCCTTGATGAATTAATGTCCAGTGGAAACCAGGTTCCTAAAACTGGGGGAGATGAGTCAGACGGTGAGAAGAAAATTTCTTTCGAGGGAGAAGAAATTGACCCACAAGATCCTGCCATTATCCAGATGATGAATGAAAAATTATATAAACAGTCGTTCAGTTCATATTACAAGTCTTGTCAAAGTTTAAGACTTTCAGATGGTTCAGGTGCCTGTAAAGAAGGTTTTCAGGCTGGTTTTAAGATGAGCACTTCTAGGACATCTCTACTTTCTGTCTCTGTTACTGATTTGGATCTAAGTTTGACAGCTATTGGTGGAGGTGAAGCTGGGATGATAGAAATTGTTAAGAAGCTTGATCCTGTAGCTGAAGAAAAAGACATACCTTTTTCGCGGCTTTATGGAAGCAATCTTCGGTTAAACACTGGAACTCTCGCTGTTCAAATACGAAATTACACATTTCCTCTTCTCTCAACAGCTTTCGGTAAATGCGAAGGGTGTCTTGTGTTGGCTCAGCAGGCAACAGCTTTTCAGCCACAAATAATCCATGACGTATACATAGGAAGATGGAGAAAGGTGCAGATGCTCCGTTCAGCGAGTGGAACAACACCCGCAATGAAAACATATCTTGACCTGCCGATAAAATTCCAGAAAGGAGAGATCTCTTTTGGAATTGGATATGAACCAGTACTTGCTGATATCAGCTATGCCTTCACTGTGGCTCTTCGTAGAGCTAATCTCAGTCTCAAGGGTCCTGGTCTTCTTCAGCCTCCTAAAAAGGAGAAAAGTTTACCATGGTGGGATGAAATGAGAAATTACGTCCATGGAAATGTAACCTTGTCCTTTTCTGAGACAAAGTGGATTGTTCTTGGTAGTCCAGATCCCTATGAGAAGCTTGACAAGCTCCACATGACCTCTGGCTCTGTGGAAATTCAGCAATATGATGGTCGTGTGCACTTCTCTGCTGAGGACATAAAGATTTTCTTTAGCAGTTTTGAAGGTTTGGCTAGGCATTATCCGAACTCTCCAGTTTGTCCATCAAGCTACCCTTTCCTTGAGGTTCCACGCTTCAGTCTGGAAGTCCGGATGGATTGGGAATGCGAGTCTGGAAGTCCACTAAATCATTATCTGTTTGCTTTACCTATTGAAGGAAAGGCTCGTGATAAAATCTATGATCCGTTCAGGTCTACTTCTCTCTCACTACGCTGGGATTTCACTTTAAGACCTGAGAACCCATCAGTATCTGCAGTTGATCAAACAAAAAAAGTTGGATCCGAGTGTAAGCCTGAGAAATCTTCTTTTTCTCCACCCACAATAAACATTGGTGCTCATGATTTGGCATGGTTGATCAGATTCTGGAACATGAATTACCTTCCTCCTTACAAACTACGCACGTTTTCCCGGTGGCCTCGCTTTGGAGTTCCAAGAATCCCAAGATCAGGAAATTTGTCCCTAGACAGAGTGATGACGGAATATAATCTCCGTTTAGATGTTACACCTATTTGTATCAAGCATATGACACTGGATTCTAATAATCCTGCCAAAGGCTTGACATTTGATATGACCAAGCTAAAGTATGAAATCTGCTTCAGCCGTGGCAACCAGGACTTTACTTTTGAATGCAAGCGTGAGACTCTTGATCCAGTGTACCAGGGAATAGACCTCCATTTGCCTAAAGCCTTTTTGAGAAGGGATCAGCATTGCTCAAAGCCAGCTCAGATGAGTCGAACAAGTTCACTATCTGGATCAACTGATAGAGTTACTTCTGATAATGGGACTTCTACCAGTGATGGCACTGAGAAGCATCCTGACGATGGATTTTTGTTTTCTTCTGATTATTTCACAATCAGGAGGCAGGCCCCTAAGGCTGACCCTGAAAGACTGATGGTATGGAAAGAAGAAGGAAAAATATATCGTGAAAAAGTAGATGCAAGATCTACAAAGGAGAAACAGAGTGAACCTGAGGAGAATTCACATTCCGATCCGAGCGATGATGATGGATACAATGTCGTAATAGCGGACAATTGTCAGCGTATTTTTGTTTACGGACTCAAACTATTATGGAACATAGAAAACAGAGATGCTGTTTTGTCTTTTGCTGGTGGGATGTCCAAGGCATTTCAACCACCCAAGCCTTCTCCATCACGTCAGTATGCTCAGAGAAAGTTACTTGAGGGCAACCAGAAGCATTCTGAATCAGAAGTTTCTCAAGATGAACCAACTAAACAGCCTTCCACAGGCAGTGGAAATCTCGCTTCCCAATCTAAGGAGCCTGCGGAAGTTCTTTCACCTTCATCAGAACCAATTAAAACAGAGAACTTTGCATCTTTCCCTCTTGGAGCCACAAAGACTGGCGATTCAAATGATCCAGAGGAAGAAGGAACCCGGCATTTTATGGTGAACGTTGTTGAGCCACAATTCAATCTCCATTCAGAAGACATAAATGGTAGATTTTTGCTCGCTGCTGCTAGTGGCCGTGTTTTAGCACGATCATTTCATTCAGTTGTTCATGTTGCGTATGACATGATTGAGAAGGCAGCTCAAAATGAAAATGATCATAATCCTGAAAATGGCACTGACATGACATGGACACGCATGGAAGTGTCCATGATGTTGGAGCATGTACAAGCTCATGTGGCACCAACAGATGTCGATCCAGGAGCTGGGGTGCAATGGCTCCCAAAGATTAGAAGAAGCTCCCCGAAGGCAAAGCGTACTGGCGCACTGCTTGAAAGGGTTTTTATGCCGTGTGACATGTACTTTCAATATACCAGGCACAAAGGTGTGACTCCCGACCTGAAGGTGAAGCCACTGAAAGAGCTTACTTTCAATTCTCGGAATATTACAGCATCGATGACATCACGTCAGTTTCAAGTGATGACAGATGTTTTGTCCAATCTTTTGTTTGCAAGGTTACCAAAGGCTCATAACGACAGTTTAAAACTTTCTGGTGAAGAAGATGATGAAGTTGAAGAGGAAATTGATGAGGTAGTTCCTGATGGCATTGAGGAAGTGGAACTTGCGAAAATTGAACTTGAAGCGAAAGAAAGGGATAGGATGATGCTTCTTGATGACATCAGAAAATTGACGCAGAACGAAAGTAACTCAGGAAACATAAATCTCGAAAAGGAAAGCGATTTCTGGATGATCAGCGGTGGGAGGCCAGTATTGGTGGAACGACTGAGGAAAGCGTATCTCAGTGTGCAACAGTCTAGGAAGACAGCATATACAGCACTGCGGACTTCTGTGAAGAATGCTGCGGAGCTAAGGCTGTTGGAAAAAGATAAGAACAAAAGACCATCCTCTGCCATGCGCATATCCTTACAAATCAACAAAGTCATCTGGTCTATGGTTTTAGATGGAAAAACATTCGCTGAGGTTGAAATAGACAATATGATATATGATTTTAACCGGGACTACAGAGATATTGGGATTGCCCAATTCACAACGAGATATGTTGTCCTGCGGAACTGTCTGCCTAATGCCAAATGTGATACTGTTCTATCAGCTTGGAATCCTCCTCCGGAATGGGGCAAGAAAGTTATGTTACAGGTAGATGCAAGACAAGGAGCCCCAAAAGATGGACAGGCTCCGTATGAACTTTTCCAGGTTGAAATATATCCTCTAAAAATCCATTTGACAGAAACAATGTATACAATGATGTGGGAGTATATCTTTCCAGGAGAGGAGCAACATTCACAGAGAAGGGAGGAAGTTTGGAAAGTGTCAACAACCTCAGGATCAAGGCGCAGGAAAGGTTCATTCGCTCAAGAAGCGGCAGCATTGCTCGCTGCATCTGATCTAGGCCAGGGGTCAAAGAATCAAAGCCTGAAGTCAAGCACGATCCGTGGATCAGGGCGAGAACTGAGAAGAACATCCTCTTTTGACAGATCATGGGAGGAGACTGTGGCAGAGTCTGTAGCTACTGAGCTTGTTCTATCTTCTATGGAGCATCAAGGTGAATCCTCAAAAGGCAAGCTGAAGGATTCAAAAACTTCCAAGGCTGGTGGCCGTTCAGTTCATGAAGAAAAAAAGGGGGAAAAGTCATTAGAAGACAAGAAATCTAGGCCTCAAAAGATAATGCAATTCCAAACCATCAAGATTAGTCAGGTGGAATTGTTGATCACTTACGAGGGCTCAAGATTTGTTGTGAACGATATGAAGCTTTGTATGGACACATTTCATCGTGTTGAATTCAGCGGAACATGGAGGAGACTCTTCTCTCGAGTAAAGAAGCACATAATATGGGGAGTCCTCAAGTCGGTAACCGGAATGCAAATGAAGAAATTCAAAGACAAAGCCCATGTTCCAAAAGATGATATTGGTTTGAGAGACAAAGACGAGTCAGGGAGAACAGATCAGGAGTCAGGGGCATGGGTGAAACGTCCTGGTGACAATGCAGGTGATGGATTTGTCACATCGATTAGAGGTATTTTCAATACACAAAGGCGAAAGGCCAAGAAATTCGTGCTTCGGACAATGAGAGGGGAAGCAGAAGACAACTTCCCTGGGGAATGGAGTGACAACGAATCAGACTTCTCTCCTTTCGCAAGACAATTGACGATTACTAAGGCTAAGAAACTCATCAGGCGTCACTCCAAGAAGTTCCAAAATCAAAACACTACAAAAGGTTCTAAGAAGACCCAACTCTCGCCTACGCTCTCTCCACCCAAGGAAGAAGACCAATACGAATCTGACTCTTCCAGTGGATCGTCTGCTTACGAAGAGTTCCTTGACCAGAATCAGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005575 GO:0005623 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0009267 GO:0009605 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010015 GO:0010053 GO:0010054 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0016036 GO:0016043 GO:0016049 GO:0021700 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030154 GO:0030307 GO:0030427 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035618 GO:0035619 GO:0035838 GO:0040007 GO:0040008 GO:0042594 GO:0042995 GO:0044463 GO:0044464 GO:0044706 GO:0045927 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051286 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070297 GO:0070298 GO:0071496 GO:0071695 GO:0071840 GO:0080147 GO:0090404 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0120038 GO:1902531 GO:1902532 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Apt1 Fmp27_GFWDK

Protein Analysis

2587

Amino Acids

290.41

Weight (kDa)

8.57

Isoelectric Point (pI)

45.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hobbit	PF10344	4 - 2511	0.0	Hobbit/FMP27/BTLP2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58250 AT1G58250 AT5G49680
fragaria_vesca	FvH4_5g34390
malus_domestica	MD08G1201600.v1.1 MD08G1201800.v1.1 MD08G1202400.v1.1 MD08G1208300.v1.1 MD15G1388100.v1.1
prunus_persica	Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1
pyrus_communis	pycom15g34840
rosa_chinensis	RchiOBHm_Chr7g0235041
rosa_laevigata	RLG00000001185
rosa_multiflora	Rmu_co8243721.1_g000001 Rmu_sc0002239.1_g000001 Rmu_sc0013687.1_g000001 Rmu_sc0015410.1_g000007
rosa_roxburghii	Rroxscaffold_3G00226360
rosa_rugosa	Rorug07G0287300
rosa_samantha	Rh7AG442100 Rh7BG414600 Rh7CG463100 Rh7DG431700
rosa_wichuraiana	Rw7G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1229, 1955
AarI	CACCTGC	1 cut(s)	7403
Acc16I	TGCGCA	1 cut(s)	6326
Acc36I	ACCTGC	2 cut(s)	3654, 7403
AccB1I	GGYRCC	2 cut(s)	3224, 5650
AccB7I	CCANNNNNTGG	2 cut(s)	5675, 6593
AccI	GTMKAC	5 cut(s)	735, 2093, 3567, 4199, 6665
AccII	CGCG	2 cut(s)	331, 3406
AccIII	TCCGGA	2 cut(s)	4095, 6535
AclI	AACGTT	2 cut(s)	1858, 5415
AcoI	YGGCCR	2 cut(s)	5485, 7021
AcyI	GRCGYC	1 cut(s)	7603
AdeI	CACNNNGTG	1 cut(s)	2177
AfeI	AGCGCT	1 cut(s)	1716
AflII	CTTAAG	1 cut(s)	2345
AflIII	ACRYGT	4 cut(s)	559, 1052, 1921, 5766
AgeI	ACCGGT	1 cut(s)	2390
AhdI	GACNNNNNGTC	2 cut(s)	496, 5874
AjiI	CACGTC	2 cut(s)	5159, 5876
AjuI	GAANNNNNNNTTGG	2 cut(s)	1533, 1565
AleI	CACNNNNGTG	2 cut(s)	1148, 5793
AloI	GAACNNNNNNTCC	2 cut(s)	3033, 3065
Alw21I	GWGCWC	6 cut(s)	359, 477, 2289, 3266, 3969, 4342
Alw26I	GTCTC	9 cut(s)	78, 1969, 3295, 3761, 3857, 4637, 6908, 7219, 7338
Alw44I	GTGCAC	2 cut(s)	2285, 3965
AlwNI	CAGNNNCTG	5 cut(s)	1370, 4256, 4368, 5675, 6929
Ama87I	CYCGRG	1 cut(s)	7236
Aor13HI	TCCGGA	2 cut(s)	4095, 6535
Aor51HI	AGCGCT	1 cut(s)	1716
AoxI	GGCC	9 cut(s)	1440, 4418, 4869, 5485, 6173, 6817, 7021, 7079, 7467
ApaLI	GTGCAC	2 cut(s)	2285, 3965
ApeKI	GCWGC	9 cut(s)	5, 772, 803, 5474, 5477, 5549, 6269, 6791, 6803
ArsI	GACNNNNNNTTYG	6 cut(s)	1442, 1474, 4610, 4642, 5884, 5916
AseI	ATTAAT	2 cut(s)	1577, 3029
AsiGI	ACCGGT	1 cut(s)	2390
Asp700I	GAANNNNTTC	4 cut(s)	509, 3240, 4744, 7298
AspLEI	GCGC	5 cut(s)	333, 1717, 5735, 6327, 6762
AspS9I	GGNCC	5 cut(s)	259, 326, 692, 3764, 4870
AsuC2I	CCSGG	4 cut(s)	2627, 4414, 5398, 6425
AvaI	CYCGRG	1 cut(s)	7236
AvaII	GGWCC	4 cut(s)	259, 326, 692, 3764
AxyI	CCTNAGG	4 cut(s)	1629, 4875, 4974, 6748
BaeGI	GKGCMC	3 cut(s)	1801, 2289, 3969
BaeI	ACNNNNGTAYC	2 cut(s)	1461, 1494
BamHI	GGATCC	1 cut(s)	4283
BanI	GGYRCC	2 cut(s)	3224, 5650
BanII	GRGCYC	4 cut(s)	359, 477, 6586, 7151
BarI	GAAGNNNNNNTAC	4 cut(s)	832, 864, 2018, 2050
BauI	CACGAG	1 cut(s)	4170
BbsI	GAAGAC	9 cut(s)	633, 1053, 3764, 5454, 6236, 7071, 7515, 7658, 7698
Bbv12I	GWGCWC	6 cut(s)	359, 477, 2289, 3266, 3969, 4342
BbvCI	CCTCAGC	5 cut(s)	1363, 2523, 2792, 3978, 6387
BbvI	GCAGC	9 cut(s)	17, 759, 790, 5461, 5464, 5561, 6256, 6790, 6803
BceAI	ACGGC	7 cut(s)	261, 1990, 2249, 4590, 5472, 5743, 7008
BcgI	CGANNNNNNTGC	2 cut(s)	1788, 1822
BciVI	GTATCC	1 cut(s)	5006
BclI	TGATCA	5 cut(s)	4264, 4359, 5566, 6159, 7134
BcnI	CCSGG	4 cut(s)	2627, 4414, 5398, 6425
BcoDI	GTCTC	9 cut(s)	78, 1969, 3295, 3761, 3857, 4637, 6908, 7219, 7338
BfmI	CTRYAG	7 cut(s)	186, 450, 2753, 3375, 4257, 6430, 6930
BfoI	RGCGCY	1 cut(s)	1718
BfrI	CTTAAG	1 cut(s)	2345
BfuAI	ACCTGC	2 cut(s)	3654, 7403
BfuI	GTATCC	1 cut(s)	5006
BglI	GCCNNNNNGGC	2 cut(s)	2749, 4877
BglII	AGATCT	3 cut(s)	669, 3672, 4946
BlpI	GCTNAGC	1 cut(s)	3525
BmcAI	AGTACT	2 cut(s)	2571, 3702
Bme18I	GGWCC	4 cut(s)	259, 326, 692, 3764
BmeRI	GACNNNNNGTC	2 cut(s)	496, 5874
BmeT110I	CYCGRG	1 cut(s)	7236
BmgBI	CACGTC	2 cut(s)	5159, 5876
BmgT120I	GGNCC	5 cut(s)	259, 326, 692, 3764, 4870
BmrI	ACTGGG	1 cut(s)	3069
BmuI	ACTGGG	1 cut(s)	3069
BoxI	GACNNNNGTC	2 cut(s)	1053, 5037
BpiI	GAAGAC	9 cut(s)	633, 1053, 3764, 5454, 6236, 7071, 7515, 7658, 7698
BplI	GAGNNNNNCTC	2 cut(s)	3735, 3767
BpmI	CTGGAG	4 cut(s)	1061, 1899, 2930, 4023
Bpu10I	CCTNAGC	6 cut(s)	1363, 2523, 2792, 3978, 6278, 6387
Bpu1102I	GCTNAGC	1 cut(s)	3525
BpuEI	CTTGAG	8 cut(s)	301, 3743, 4088, 5204, 5205, 6765, 7135, 7253
BpuMI	CCSGG	4 cut(s)	2627, 4414, 5398, 6425
Bsa29I	ATCGAT	2 cut(s)	5864, 7433
BsaBI	GATNNNNATC	3 cut(s)	3306, 5571, 5664
BsaHI	GRCGYC	1 cut(s)	7603
BsaWI	WCCGGW	4 cut(s)	2390, 4095, 6535, 7280
BsaXI	ACNNNNNCTCC	6 cut(s)	2075, 2105, 5664, 5694, 7660, 7690
Bse118I	RCCGGY	1 cut(s)	2390
Bse21I	CCTNAGG	4 cut(s)	1629, 4875, 4974, 6748
Bse3DI	GCAATG	7 cut(s)	155, 658, 1308, 3633, 4711, 5690, 6794
Bse8I	GATNNNNATC	3 cut(s)	3306, 5571, 5664
BseAI	TCCGGA	2 cut(s)	4095, 6535
BseCI	ATCGAT	2 cut(s)	5864, 7433
BseJI	GATNNNNATC	3 cut(s)	3306, 5571, 5664
BseMI	GCAATG	7 cut(s)	155, 658, 1308, 3633, 4711, 5690, 6794
BseRI	GAGGAG	5 cut(s)	4991, 6522, 6713, 6926, 7237
BseSI	GKGCMC	3 cut(s)	1801, 2289, 3969
BseXI	GCAGC	9 cut(s)	17, 759, 790, 5461, 5464, 5561, 6256, 6790, 6803
BseYI	CCCAGC	7 cut(s)	1204, 2504, 2648, 2680, 3341, 4218, 5675
BsgI	GTGCAG	3 cut(s)	455, 2549, 3611
Bsh1236I	CGCG	2 cut(s)	331, 3406
Bsh1285I	CGRYCG	1 cut(s)	36
BshFI	GGCC	9 cut(s)	1442, 4420, 4871, 5487, 6175, 6819, 7023, 7081, 7469
BshNI	GGYRCC	2 cut(s)	3224, 5650
BshTI	ACCGGT	1 cut(s)	2390
BshVI	ATCGAT	2 cut(s)	5864, 7433
BsiEI	CGRYCG	1 cut(s)	36
BsiHKAI	GWGCWC	6 cut(s)	359, 477, 2289, 3266, 3969, 4342
BsiHKCI	CYCGRG	1 cut(s)	7236
BsiSI	CCGG	9 cut(s)	91, 2391, 2626, 4096, 4414, 5398, 6424, 6536, 7281
BslFI	GGGAC	6 cut(s)	1843, 2308, 2707, 4447, 4801, 6440
BsmAI	GTCTC	9 cut(s)	78, 1969, 3295, 3761, 3857, 4637, 6908, 7219, 7338
BsmFI	GGGAC	6 cut(s)	1843, 2308, 2707, 4447, 4801, 6440
BsmI	GAATGC	6 cut(s)	2907, 4115, 4637, 5200, 6271, 7290
BsnI	GGCC	9 cut(s)	1442, 4420, 4871, 5487, 6175, 6819, 7023, 7081, 7469
BsoBI	CYCGRG	1 cut(s)	7236
Bsp1286I	GDGCHC	9 cut(s)	359, 477, 1801, 2289, 3266, 3969, 4342, 6586, 7151
Bsp13I	TCCGGA	2 cut(s)	4095, 6535
Bsp1407I	TGTACA	3 cut(s)	541, 1477, 5635
Bsp1720I	GCTNAGC	1 cut(s)	3525
Bsp19I	CCATGG	4 cut(s)	1068, 2699, 3806, 3835
BspANI	GGCC	9 cut(s)	1442, 4420, 4871, 5487, 6175, 6819, 7023, 7081, 7469
BspDI	ATCGAT	2 cut(s)	5864, 7433
BspEI	TCCGGA	2 cut(s)	4095, 6535
BspFNI	CGCG	2 cut(s)	331, 3406
BspHI	TCATGA	3 cut(s)	513, 4342, 7033
BspMAI	CTGCAG	1 cut(s)	4261
BspMI	ACCTGC	2 cut(s)	3654, 7403
BspQI	GCTCTTC	2 cut(s)	2249, 3741
BspT107I	GGYRCC	2 cut(s)	3224, 5650
BspTI	CTTAAG	1 cut(s)	2345
BsrDI	GCAATG	7 cut(s)	155, 658, 1308, 3633, 4711, 5690, 6794
BsrFI	RCCGGY	1 cut(s)	2390
BsrGI	TGTACA	3 cut(s)	541, 1477, 5635
BssAI	RCCGGY	1 cut(s)	2390
BssNAI	GTATAC	2 cut(s)	3568, 6666
BssNI	GRCGYC	1 cut(s)	7603
BssSI	CACGAG	1 cut(s)	4170
BssT1I	CCWWGG	9 cut(s)	80, 1068, 1146, 2699, 3806, 3835, 5124, 7011, 7683
Bst1107I	GTATAC	2 cut(s)	3568, 6666
Bst2BI	CACGAG	1 cut(s)	4170
Bst6I	CTCTTC	8 cut(s)	2249, 2367, 3483, 3741, 5979, 7235, 7717, 7731
BstACI	GRCGYC	1 cut(s)	7603
BstAFI	CTTAAG	1 cut(s)	2345
BstAPI	GCANNNNNTGC	1 cut(s)	896
BstAUI	TGTACA	3 cut(s)	541, 1477, 5635
BstDSI	CCRYGG	6 cut(s)	1068, 2699, 3806, 3835, 4606, 6859
BstEII	GGTNACC	2 cut(s)	5925, 7276
BstENI	CCTNNNNNAGG	3 cut(s)	4158, 4554, 5247
BstFNI	CGCG	2 cut(s)	331, 3406
BstH2I	RGCGCY	1 cut(s)	1718
BstHHI	GCGC	5 cut(s)	333, 1717, 5735, 6327, 6762
BstMAI	GTCTC	9 cut(s)	78, 1969, 3295, 3761, 3857, 4637, 6908, 7219, 7338
BstMCI	CGRYCG	1 cut(s)	36
BstNSI	RCATGY	7 cut(s)	563, 899, 1056, 2656, 2743, 5636, 5770
BstPAI	GACNNNNGTC	2 cut(s)	1053, 5037
BstPI	GGTNACC	2 cut(s)	5925, 7276
BstSFI	CTRYAG	7 cut(s)	186, 450, 2753, 3375, 4257, 6430, 6930
BstSLI	GKGCMC	3 cut(s)	1801, 2289, 3969
BstUI	CGCG	2 cut(s)	331, 3406
BstV1I	GCAGC	9 cut(s)	17, 759, 790, 5461, 5464, 5561, 6256, 6790, 6803
BstV2I	GAAGAC	9 cut(s)	633, 1053, 3764, 5454, 6236, 7071, 7515, 7658, 7698
BstX2I	RGATCY	8 cut(s)	669, 1564, 3124, 3307, 3672, 3892, 4283, 4946
BstXI	CCANNNNNNTGG	2 cut(s)	2706, 5626
BstYI	RGATCY	8 cut(s)	669, 1564, 3124, 3307, 3672, 3892, 4283, 4946
BstZ17I	GTATAC	2 cut(s)	3568, 6666
Bsu15I	ATCGAT	2 cut(s)	5864, 7433
Bsu36I	CCTNAGG	4 cut(s)	1629, 4875, 4974, 6748
BsuI	GTATCC	1 cut(s)	5006
BsuRI	GGCC	9 cut(s)	1442, 4420, 4871, 5487, 6175, 6819, 7023, 7081, 7469
BsuTUI	ATCGAT	2 cut(s)	5864, 7433
BtgI	CCRYGG	6 cut(s)	1068, 2699, 3806, 3835, 4606, 6859
BtgZI	GCGATG	2 cut(s)	1650, 5016
BtrI	CACGTC	2 cut(s)	5159, 5876
BtsI	GCAGTG	4 cut(s)	1443, 5260, 5735, 6245
BveI	ACCTGC	2 cut(s)	3654, 7403
CaiI	CAGNNNCTG	5 cut(s)	1370, 4256, 4368, 5675, 6929
CciI	TCATGA	3 cut(s)	513, 4342, 7033
CfoI	GCGC	5 cut(s)	333, 1717, 5735, 6327, 6762
Cfr10I	RCCGGY	1 cut(s)	2390
Cfr13I	GGNCC	5 cut(s)	259, 326, 692, 3764, 4870
ClaI	ATCGAT	2 cut(s)	5864, 7433
CseI	GACGC	2 cut(s)	6109, 7592
CsiI	ACCWGGT	1 cut(s)	3045
CspAI	ACCGGT	1 cut(s)	2390
CspCI	CAANNNNNGTGG	2 cut(s)	7000, 7035
DraI	TTTAAA	3 cut(s)	205, 756, 5952
DraIII	CACNNNGTG	1 cut(s)	2177
DriI	GACNNNNNGTC	2 cut(s)	496, 5874
EaeI	YGGCCR	2 cut(s)	5485, 7021
Eam1104I	CTCTTC	8 cut(s)	2249, 2367, 3483, 3741, 5979, 7235, 7717, 7731
Eam1105I	GACNNNNNGTC	2 cut(s)	496, 5874
EarI	CTCTTC	8 cut(s)	2249, 2367, 3483, 3741, 5979, 7235, 7717, 7731
Ecl136II	GAGCTC	2 cut(s)	357, 475
Eco130I	CCWWGG	9 cut(s)	80, 1068, 1146, 2699, 3806, 3835, 5124, 7011, 7683
Eco147I	AGGCCT	1 cut(s)	7081
Eco24I	GRGCYC	4 cut(s)	359, 477, 6586, 7151
Eco32I	GATATC	2 cut(s)	2637, 3712
Eco47I	GGWCC	4 cut(s)	259, 326, 692, 3764
Eco47III	AGCGCT	1 cut(s)	1716
Eco53kI	GAGCTC	2 cut(s)	357, 475
Eco81I	CCTNAGG	4 cut(s)	1629, 4875, 4974, 6748
Eco88I	CYCGRG	1 cut(s)	7236
Eco91I	GGTNACC	2 cut(s)	5925, 7276
EcoICRI	GAGCTC	2 cut(s)	357, 475
EcoNI	CCTNNNNNAGG	3 cut(s)	4158, 4554, 5247
EcoO109I	RGGNCCY	3 cut(s)	259, 3764, 4870
EcoO65I	GGTNACC	2 cut(s)	5925, 7276
EcoRI	GAATTC	3 cut(s)	2428, 4981, 7205
EcoRV	GATATC	2 cut(s)	2637, 3712
EcoT14I	CCWWGG	9 cut(s)	80, 1068, 1146, 2699, 3806, 3835, 5124, 7011, 7683
EcoT22I	ATGCAT	3 cut(s)	1089, 1511, 2741
EcoT38I	GRGCYC	4 cut(s)	359, 477, 6586, 7151
ErhI	CCWWGG	9 cut(s)	80, 1068, 1146, 2699, 3806, 3835, 5124, 7011, 7683
FaqI	GGGAC	6 cut(s)	1843, 2308, 2707, 4447, 4801, 6440
FauI	CCCGC	1 cut(s)	3631
FauNDI	CATATG	1 cut(s)	4527
FbaI	TGATCA	5 cut(s)	4264, 4359, 5566, 6159, 7134
FblI	GTMKAC	5 cut(s)	735, 2093, 3567, 4199, 6665
FriOI	GRGCYC	4 cut(s)	359, 477, 6586, 7151
FspAI	RTGCGCAY	1 cut(s)	6326
FspI	TGCGCA	1 cut(s)	6326
GlaI	GCGC	5 cut(s)	332, 1716, 5734, 6326, 6761
GsaI	CCCAGC	7 cut(s)	1208, 2508, 2652, 2684, 3345, 4222, 5679
GsuI	CTGGAG	4 cut(s)	1061, 1899, 2930, 4023
HaeII	RGCGCY	1 cut(s)	1718
HaeIII	GGCC	9 cut(s)	1442, 4420, 4871, 5487, 6175, 6819, 7023, 7081, 7469
HapII	CCGG	9 cut(s)	91, 2391, 2626, 4096, 4414, 5398, 6424, 6536, 7281
HgaI	GACGC	2 cut(s)	6109, 7592
HhaI	GCGC	5 cut(s)	333, 1717, 5735, 6327, 6762
Hin1I	GRCGYC	1 cut(s)	7603
Hin6I	GCGC	5 cut(s)	331, 1715, 5733, 6325, 6760
HinP1I	GCGC	5 cut(s)	331, 1715, 5733, 6325, 6760
HincII	GTYRAC	3 cut(s)	1669, 2473, 6741
HindII	GTYRAC	3 cut(s)	1669, 2473, 6741
HindIII	AAGCTT	6 cut(s)	938, 2804, 2988, 3365, 3905, 7175
HpaII	CCGG	9 cut(s)	91, 2391, 2626, 4096, 4414, 5398, 6424, 6536, 7281
Hpy99I	CGWCG	3 cut(s)	50, 731, 2208
Hsp92I	GRCGYC	1 cut(s)	7603
HspAI	GCGC	5 cut(s)	331, 1715, 5733, 6325, 6760
Kpn2I	TCCGGA	2 cut(s)	4095, 6535
Ksp22I	TGATCA	5 cut(s)	4264, 4359, 5566, 6159, 7134
LguI	GCTCTTC	2 cut(s)	2249, 3741
Lsp1109I	GCAGC	9 cut(s)	17, 759, 790, 5461, 5464, 5561, 6256, 6790, 6803
MabI	ACCWGGT	1 cut(s)	3045
MfeI	CAATTG	3 cut(s)	1004, 5035, 7568
MflI	RGATCY	8 cut(s)	669, 1564, 3124, 3307, 3672, 3892, 4283, 4946
MhlI	GDGCHC	9 cut(s)	359, 477, 1801, 2289, 3266, 3969, 4342, 6586, 7151
MmeI	TCCRAC	4 cut(s)	450, 4261, 5607, 6267
Mph1103I	ATGCAT	3 cut(s)	1089, 1511, 2741
MroI	TCCGGA	2 cut(s)	4095, 6535
MroXI	GAANNNNTTC	4 cut(s)	509, 3240, 4744, 7298
MslI	CAYNNNNRTG	8 cut(s)	1148, 1229, 2294, 3840, 5591, 5612, 5793, 6969
MspA1I	CMGCKG	4 cut(s)	1249, 1970, 6165, 7212
MspCI	CTTAAG	1 cut(s)	2345
MspI	CCGG	9 cut(s)	91, 2391, 2626, 4096, 4414, 5398, 6424, 6536, 7281
MunI	CAATTG	3 cut(s)	1004, 5035, 7568
Mva1269I	GAATGC	6 cut(s)	2907, 4115, 4637, 5200, 6271, 7290
MvnI	CGCG	2 cut(s)	331, 3406
NciI	CCSGG	4 cut(s)	2627, 4414, 5398, 6425
NcoI	CCATGG	4 cut(s)	1068, 2699, 3806, 3835
NdeI	CATATG	1 cut(s)	4527
NmeAIII	GCCGAG	1 cut(s)	1213
NsbI	TGCGCA	1 cut(s)	6326
NsiI	ATGCAT	3 cut(s)	1089, 1511, 2741
NspI	RCATGY	7 cut(s)	563, 899, 1056, 2656, 2743, 5636, 5770
OliI	CACNNNNGTG	2 cut(s)	1148, 5793
PaeI	GCATGC	3 cut(s)	899, 2656, 2743
PaeR7I	CTCGAG	1 cut(s)	7236
PagI	TCATGA	3 cut(s)	513, 4342, 7033
PaqCI	CACCTGC	1 cut(s)	7403
PasI	CCCWGGG	1 cut(s)	7520
PceI	AGGCCT	1 cut(s)	7081
PciI	ACATGT	3 cut(s)	559, 1052, 5766
PciSI	GCTCTTC	2 cut(s)	2249, 3741
PcsI	WCGNNNNNNNCGW	2 cut(s)	42, 3501
PctI	GAATGC	6 cut(s)	2907, 4115, 4637, 5200, 6271, 7290
PdmI	GAANNNNTTC	4 cut(s)	509, 3240, 4744, 7298
PflMI	CCANNNNNTGG	2 cut(s)	5675, 6593
PfoI	TCCNGGA	4 cut(s)	255, 1077, 5668, 6691
PinAI	ACCGGT	1 cut(s)	2390
Ple19I	CGATCG	1 cut(s)	36
PpuMI	RGGWCCY	2 cut(s)	259, 3764
PscI	ACATGT	3 cut(s)	559, 1052, 5766
PshAI	GACNNNNGTC	2 cut(s)	1053, 5037
PshBI	ATTAAT	2 cut(s)	1577, 3029
PsiI	TTATAA	2 cut(s)	1229, 1955
Psp124BI	GAGCTC	2 cut(s)	359, 477
Psp1406I	AACGTT	2 cut(s)	1858, 5415
Psp5II	RGGWCCY	2 cut(s)	259, 3764
PspEI	GGTNACC	2 cut(s)	5925, 7276
PspFI	CCCAGC	7 cut(s)	1204, 2504, 2648, 2680, 3341, 4218, 5675
PspPI	GGNCC	5 cut(s)	259, 326, 692, 3764, 4870
PspPPI	RGGWCCY	2 cut(s)	259, 3764
PsrI	GAACNNNNNNTAC	2 cut(s)	4364, 4396
PstI	CTGCAG	1 cut(s)	4261
PstNI	CAGNNNCTG	5 cut(s)	1370, 4256, 4368, 5675, 6929
PsuI	RGATCY	8 cut(s)	669, 1564, 3124, 3307, 3672, 3892, 4283, 4946
PvuI	CGATCG	1 cut(s)	36
PvuII	CAGCTG	1 cut(s)	1970
RseI	CAYNNNNRTG	8 cut(s)	1148, 1229, 2294, 3840, 5591, 5612, 5793, 6969
SacI	GAGCTC	2 cut(s)	359, 477
SapI	GCTCTTC	2 cut(s)	2249, 3741
Sau96I	GGNCC	5 cut(s)	259, 326, 692, 3764, 4870
ScaI	AGTACT	2 cut(s)	2571, 3702
SduI	GDGCHC	9 cut(s)	359, 477, 1801, 2289, 3266, 3969, 4342, 6586, 7151
SexAI	ACCWGGT	1 cut(s)	3045
SfcI	CTRYAG	7 cut(s)	186, 450, 2753, 3375, 4257, 6430, 6930
Sfr274I	CTCGAG	1 cut(s)	7236
SinI	GGWCC	4 cut(s)	259, 326, 692, 3764
SlaI	CTCGAG	1 cut(s)	7236
SmiMI	CAYNNNNRTG	8 cut(s)	1148, 1229, 2294, 3840, 5591, 5612, 5793, 6969
SphI	GCATGC	3 cut(s)	899, 2656, 2743
SseBI	AGGCCT	1 cut(s)	7081
SspI	AATATT	4 cut(s)	394, 2260, 2922, 5854
SstI	GAGCTC	2 cut(s)	359, 477
StuI	AGGCCT	1 cut(s)	7081
StyI	CCWWGG	9 cut(s)	80, 1068, 1146, 2699, 3806, 3835, 5124, 7011, 7683
TatI	WGTACW	7 cut(s)	541, 1477, 2034, 2569, 3700, 5635, 5769
TauI	GCSGC	5 cut(s)	1249, 1425, 1816, 3409, 6791
TseI	GCWGC	9 cut(s)	5, 772, 803, 5474, 5477, 5549, 6269, 6791, 6803
TspGWI	ACGGA	8 cut(s)	2750, 3590, 4179, 4484, 4496, 5073, 6594, 6848
Van91I	CCANNNNNTGG	2 cut(s)	5675, 6593
Vha464I	CTTAAG	1 cut(s)	2345
VneI	GTGCAC	2 cut(s)	2285, 3965
VpaK11BI	GGWCC	4 cut(s)	259, 326, 692, 3764
VspI	ATTAAT	2 cut(s)	1577, 3029
XagI	CCTNNNNNAGG	3 cut(s)	4158, 4554, 5247
XbaI	TCTAGA	1 cut(s)	1268
XceI	RCATGY	7 cut(s)	563, 899, 1056, 2656, 2743, 5636, 5770
XcmI	CCANNNNNNNNNTGG	2 cut(s)	424, 3926
XhoI	CTCGAG	1 cut(s)	7236
XmiI	GTMKAC	5 cut(s)	735, 2093, 3567, 4199, 6665
XmnI	GAANNNNTTC	4 cut(s)	509, 3240, 4744, 7298
ZrmI	AGTACT	2 cut(s)	2571, 3702
Zsp2I	ATGCAT	3 cut(s)	1089, 1511, 2741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.