Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0006211

Physical Location & Seq

Genomic Coordinates Forward (+)

59712622 .. 59732592

Gene Structure 19971 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21065

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7077 bp

ATGAGTGATGCGATCTCAAAATGCACCACTTTATTTCCTGAAAAGGTTTCCTTCAATTTGCCAAAACTGGATGTGAGATTTGAGCATCGGGAACATGATTTTGCTCTTGAGAATAACATCATGGGCATCCAATTGAAAAGCATCAAATCACAATCAAGTGAAGATGTGGGAGAAAGCACGCGCCTTGATGTTCAATTAGAATTCAGCGAGATTCATCTGCTTAGAGAAGCTGGCACTTCTGTTTTGGAGATACTGAAACTTGATGTTATCTCATTTTTGTACATTCCAATACAGCCAAGCTCTCCTATTAGAGCTGAAATTGATGTAAAGCTGGGTGGTACTCAATGCAACCTTATAGTGAACAGATTAAAGCCATTGTTGCGCCTCCACTTCTCGAAAAAGAAAAGAATGGTACTTCGGGATGAAAAGCCTACTCTTGACAAGACATCTCCAACAATGGATACCAATATTATCATGTGGACATGTACTTTTTCAGCCCCTGAGATGACAATTGTGCTTCATAGCCTCAGTGGTTTGCCACTCTATCATGTCTGCTCACAATCATCACATGTATTTGCAAATAACATATCAAATACTGGAACTACTGTACATATGGAGCTCGGTGAACTTAATTTGCATATGGCGGATGAATACCAACAATGCTTGAAAGAAAACCTTTTTGGTGTGGAATCAAATTCAGGTTCCATAGTCAATGTTGCAAAGGTTAGTCTAGATTGGGGCAAAAAGGACATGGAATCATCTGAAGAAGGTGCTTCTAAAAGTAAATTAGTTTTATCTGTTGATGTGACTGGTATGGCTGTTTATTTTACTTTCAAGCGTGTTGAGTCGTTTATATCAACTGCCATGTCATTTCAAGCACTTTTTAAGAATTTGTCGTCTTCTGAAAAAAGAACATCACAAAGCCGAGGAGGGCGATCATCGAAATCCTCAGGGAAAGGGACTCGGCTATTGAAATTAAATCTTGAACGGTGTTCTTTGAATTTCTGTGGTGAGGTGGGTTTAGAAAAAATGGTTGTTCCAGATCCTAAGCGTGTAAATTATGGGTCACAAGGTGGTCGAATTGTTATAAGTGAGTCGGCTGATGGCACTCAACGGGTTGCAGAAGTAATTTCCACAGTATCTGATGATTGTAAGAACTTAAAGTACTCCATTTCTCTTGACATCTTCCATTTCAGCTTATGTGTGAACAAGGAAAAACAATCCACCCAGGTTGAACTTGAAAGAGCCAGATCTATCTATCAGGACTACTTGGAGGAACATAAGCCCGAACCAAAATTGGTACTGTTTGATATGCAGAATGCTAAATTTGTACGACGTTCTGGTGGTCTTAAAGAGATTGCTGTCTGCTCTCTTTTCAGTGCTACTGATATTACGATTAGATGGGAGCCTGATGTACATTTATCACTAATTGAACTTGGACTTCGATTGAAGTTACTGGTGCACAATCAGAAGCTTCAGGTACACGGTAAAGAACATATGGAAAATGTATCTAGCATGAGCAACTCCGAACAGAAGAAAGAAGCCATTACAGAACCAGTAAATTTAGACAAGCAAAAGAAAAGGGAATCTATCTTTGCTGTTGATGTGGAAATGCTGAATGTTTATGCTGAGGTTGGAGATGGAGTTGATGCAGTAGTCCAGGTGCAGTCAATATTTTCTGAGAATGCTCGCATAGGAGTACTTCTGGAAGGATTTCTGCTTTGTTTCAATGGGTGCAGAATACTCAAAAGTAGCCGGATGCAAATTTCATGTATTCCTAGTGCCTCTTGTGCCCCTGATGCGAAAATTCCTCCATCTACCACATGGGATTGGGTAATTCAAGGCCTTGATGTTCATATTTGCTTACCATACAGGTTAGAATTGCGTGCCATTGATGATTCTGTTGAAGAGATGCTGCGTGCTCTAAAGCTTGTAGCTGCTGCTAAAACTAGTCTTATCTTTCCGGTGAAGAAAGATCTTTCAAAAGCTAAGAAGCCCAGTTCAGTAAAATTTGGGTGTTTAAAGTTTTTAATACGGAAGTTAACTTTTGATATTGAAGAAGAACCACTGCAAGGTTGGCTTGATGAGCACTATCTCTTAATGAAGAATGAGGCATCTGAGTTAGCTGTTAGGTTGAAGTTGCTTGATGAATTTATTTCCAAAGTCAGCCAGTCTCCCAAGCCCACTGAAACAAATGATTCTACTCGAGAAAGAAAGACTTTCTTTAATGGAGTTGAGATTGACATACAAGATCCTTCAGCAGTTAGTAAGATGCAAGAAGAAATTTATAAACAATCATTCAGATCTTATTACAAGGCATGTCAGAATCTAGCACAATCAGAGGGTTCAGGTGCTTGTAGGGAAGGGCTTCAGGCTGGTTTTAAGCCTAGTACATCCAGAACTTCTCTTCTGTCTATATCAGCAACAGATTTAGATGTAAGCTTGACATTGATTGATGGTGGTGATGATGGGATGATAGATGTTATAAAGATGCTGGATCCTGTCTGTCGTGAAAATAATATACCATTTTCTAAACTTTATGGGGGTAATATTCTCCTGCACACAGGCTCACTTGTGGTTCAGCTACGTGACTTTACATTTCCTCTCTTGTCCGGAACTTCTGGTAAATGTGAAGGTCGCCTTGTGCTAGGACAGCAGGCAACAAGTTTTCAGCCTCAAGTACACAAAGATGTCTACATTGGGAAATGGAGAAAGGTGCACTTGCTTCGTTCTGCTACTGGTACAACTCCACCTATGAAAACATTCACAGATTTGTCCCTACGTTTTCAGAAAGCCGAGGTGTCCTTTGGAGTGGGATATGAACCACCTTTCGCTGATGTGAGTTATGCGTTTACCGTGGCTCTTCGCAGGGCTAATCTATGCATTAGGGACCCTAATCCACAACCCTTGCCGCCAAAGAAGGAAAAGAGCTTGCCATGGTGGGATGATATGAGAAACTACATTCATGGAAACATCAAGATTCTCTTTTCTGAAACCATATGGAATATTCTTGCGACTACTGATCCGTATGAAAAGCTTGACAAACTTCAAGTAACAGCTAGTCCTATGGAGATCCAGCAGTCAGATGGTCGTATTCATGTTTCTGCGAATGATTTTAAGGTTTTCTCAAGCAGTTTGGACACTCTGGCAAATAATCGTGGTTTAAAACTTCCTAAAGGTATATCTGGTCCTGTAATAGAAGCTCCAGCATTAACTGTTGAAGTTACAATGGATTGGGAATGTGAGTCTGGAAATCCTATGGATCATTATTTATTTGCACTTCCTATTGAAGGGAAACCTCGTGAAACGGTGTTTGATCCATTCAGATCCACCTCTCTCTCCCTTCGCTGGAATATCTTGCTTAGACGCTCTCCCTTACGCGAGAAACAAGCTTCACATTCTAATGCGGTAGATGGTGTAGATGTAGATGGAACTGTTTACGGTCCTCCTCATAAGGAAGAGAATGTTTCCAGTCTTCCACCAACAGTGAATGTTGGCGCTCATGATCTAGCATGGATTCTGAAGTTTTATAACATGCATTATCTTCCTCCTCACAAATTGCGGGCCTTTGCTCGTTTTCCTCGTTTTGGAGTTCCAAGAATCCCTAGATCCGGTAACTTGTCCTTAGACAGAGTGATGACAGAATTTATGCTCCGCATTGATGCTTCGCCTACTTGTATAAAGCATGTACCTTTAGATGATGACGATCCAGCTAAAGGACTGACATTCTTAATGACAAAGTTGAAATTGGAAATGTGTTCGAGCCGGGGTAAGCAAAAGTATACTTTTGATTGCAAGCGTGCACCTCTTGATCTTGTTTACCAGGGCCTTGACCTTCATATGCCCAAGGCATTTTTAAACAGAGAAGAAAGCACTAGTGTTGCAAAAGTAGTTCAAATGACGATAAAAAACACACAACCTGCATCAGTAGATAGAGTTCCAACTGAAAAGAGTAGCAACATGAGTAGTGGCACAGAGAAGCATCGTGATGATGGATTTCTATTGTCATCAGAGTATTTCACGATCAGAAGGCAGGCCCCAAAGGCTGATCCTGTTAGTTTATTAGCTTGGCAAGAGGCTGGAAGAAAAAATCTTGAGATGACTTATGTGAGGTCTGAATTTGAAAATGGAAGTGAGAGTGACGAGCATACCCGATCTGATCCAAGTGATGATGATGGATACAATGTGGTAATAGCTGACAATTGCCAACGTATATTTGTGTATGGACTCAAACTTCTGTGGAATATTGAGAATAGAGATGCTGTTTGGTCCTTTGTTGGTGGTCTATCCAAAGCATTTCAAGCCCCCAAACCTTCTCCCTCTAGGCAGTATGCACAAAAGAAATTACTGGAGGAGCAGCAGTCACATAGTGGAGGTGAAATGCAGCAAGATGGTAGCTCTAAGCCCACCACTACCAGCCATGGTGCTGCTTCTGCAGAGGTATCTGGTTCACTATCATCTCCGTCACCTTCCGTCAAATCGGAGATCTTGTCATCTGCAGTTGACGACTCTTCATCTGGTGTAGTAGTAGAAAAGCATAGAGAGACCAAAGATGCAGAGGAGGACGGGACTCGTCATTTCATGGTGAATGTTATTGAGCCCCAGTTCAATCTTCATTCTGAAGATGCCAATGGAAGGTTTTTGCTTGCTGCTGTTAGTGGCCGTGTTTTGGCCCGGTCATTTCATTCAGTTCTTCATGTTGGCTATGAGATGATTGAGAAGCTTCGTAATGATAATTTAAATATACCAGAATGTGAACCAGAAATGACATGGAAACGCATGGAGTTTTCTGTAATGTTAGAGCATGTTCAAGCTCATGTTGCTCCAACAGATGTTGATCCTGGAGCTGGATTGCAGTGGCTTCCAAAAATTCGTAGAAGCTCTCCAAAAGTAAAACGTACTGGTGCTTTACTTGAAAGGGTATTTATGCCCTGTGACATGTACTTTCGCTACACAAGGCACAAAGGTGGAACTCCAGAGTTGAAGGTGAAGCCCTTAAAAGAGCTGACTTTCAATTCCCATAATATCACGGCAACAATGACCTCTCGCCAATTTCAAGTTATGCTAGATGTGTTGACAAATCTTCTTTTTGCACGACTTCCCAAGCCTAGAAAAAGTAGTCTGTCACTGCCTGCTGAAGATGATGAAGATGTGGAGGAGGAGTCAGACGAAGTGGTTCCTGATGGTGTTGAAGAGGTGGAGCTTGCAAGAATAGAACTTGAGAAAAAGGAACGGGAGCAGAGATTGATTCTTGGGGACATAAGAAAGTTATCCCTTCACTGTGATACCACTGGAGACCTATATCCAGAAAAGGAAGGGGACTTGTGGATGATAAGTTGTACAAGATCTACTCTGGTGCAAGGACTAAAGAGAGAGCTGGTTAATTCAAAAAAATCTAGGAAAGCTGCGTATGCTTCATTAAGAATGGCTCTACATAAGGCTGCTCAGCTACGGCTAATGGAAAAGGAGAAAAACAAAAGTCCATCCTATGCTATGCGCATTTCTTTGCAAATTAACAAAGTGGTTTGGAGCATGCTTGTTGATGGTAAATCTTTTGCCGAGGCGGAGATCAATGACATGATTTATGACTTCGACCGAGATTACAAGGATGTTGGTGTTGCTCAGTTTACAACAAAAAATTTTGTTGTTAGAAACTGCCTCCCAAATGCCAAATCAGATATGCTTTTATCAGCATGGAATCCTCCACCTGAATGGGGAAAAAAAGTTATGCTTCGTGTGGATGCAAAGCAGGGAGCTCCAAAGGATGGAAGTTCTCCTTTGGAACTTTTTGAGGTAGAGATTTATCCTCTCAAGATCCATTTAACAGAAACAATGTACAGAATGATGTGGGGATATTTATTCCCAGAAGAAGAACAGGATTCACAAAGACGGCAGGAAGTTTGGAAGATCTCAACAACTACTGGTACAAAACGTGGAAAGAAAGCTTCTTTAGTCCCTGACATGTCTGCATTTAGCAGTCAAACAATGAAGGAGTCTGAGGGGTCATCCAAATTAAGTGCCTTGGCCCCTGGTACCAGTCAATCCCCTGTTCCTGCAGATTCTGTACAAGAATCAAAATTGCAAAGTAAAGCACTCATTGCGAGTGGTCCAAATCCTGAGTTAAGAAGAACATCTTCCTTTGACAGATCATGGGAAGAGACTGTTGCAGAATCAGTTGCTACTGAACTTGTCTTACAGAGCATTAGTGGGCCTCTTGGGTCTATTGAACAAGATGAATCCTCTAAGAATAAATTGAAAGATCCCAAAGCTATCAAATCTGGCAGGTCATCCCATGAAGAAAAGAAGGTACAAAAGTCACAAGAAGAGAAAAAATCTGCCAGGCCTCGAAAGATGATGGAGTTTCACAATATCAAGATAAGCCAGGTTGAGCTATGTGTTACCTATGAAGGATCTAGATTTGTGGTAAATGATCTCAAGTTGCTGATGGATACATTTCATCGTGTTGAGTTTACTGGTACTTGGCGGAGACTTTTTTCACGTGTTAAGAAGCACATCATTTGGGGAGTCCTGAAGTCTGTAACTGGAATGCAGGGTAAGAAGTTTAAAGACAAGGCTAACAGTCAGAGAGAACCCAGTGGATCTGGGGTTCCCGATAGTGAGCTTAATTTCAGCGACAATGAAGGCCAGCCAGGACAATCTGATCAACATCCAATAACCTTTCTCAAGCGCCCAACTGATGGAGCAGGTGATGGATTTGTCACATCCATCAGAGGCCTTTTCAATACTCAGCGTCGTAAGGCCAAGGCATTTGTGCTGCGGACCATGAGGGGTGAAGCTGAGAATGATTTCCAAGGAGATTGGAGTGAAAGTGATGCAGAGTTTTCTCCCTTTGCTAGGCAGCTCACTATAACAAAAGCTAAGAGGCTTATCAGGCGGCACACGAAGAAATTCCGTTCAAGAAAAGGTTCGTCTTCACAACAGAGAGAATCTCTTCCAACATCACCGAGGGAGACCACTCCTTTAGAAAGTGATTCTTCTGGTGGATCCTCACCCTTTGAGGATTTTAACGATGGATTTAATGAGGGCAACATGTTGGTTTCCAAAGAGGCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005575 GO:0005623 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0009267 GO:0009605 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010015 GO:0010053 GO:0010054 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0016036 GO:0016043 GO:0016049 GO:0021700 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030154 GO:0030307 GO:0030427 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035618 GO:0035619 GO:0035838 GO:0040007 GO:0040008 GO:0042594 GO:0042995 GO:0044463 GO:0044464 GO:0044706 GO:0045927 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051286 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070297 GO:0070298 GO:0071496 GO:0071695 GO:0071840 GO:0080147 GO:0090404 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0120038 GO:1902531 GO:1902532 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Apt1 Fmp27_GFWDK

Protein Analysis

2358

Amino Acids

264.74

Weight (kDa)

7.87

Isoelectric Point (pI)

51.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hobbit	PF10344	1 - 2275	0.0	Hobbit/FMP27/BTLP2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58250 AT1G58250 AT5G49680
fragaria_vesca	FvH4_5g34390
malus_domestica	MD08G1201600.v1.1 MD08G1201800.v1.1 MD08G1202400.v1.1 MD08G1208300.v1.1 MD15G1388100.v1.1
prunus_persica	Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1
pyrus_communis	pycom15g34840
rosa_chinensis	RchiOBHm_Chr7g0235041
rosa_laevigata	RLG00000001185
rosa_multiflora	Rmu_co8243721.1_g000001 Rmu_sc0002239.1_g000001 Rmu_sc0013687.1_g000001 Rmu_sc0015410.1_g000007
rosa_roxburghii	Rroxscaffold_3G00226360
rosa_rugosa	Rorug07G0287300
rosa_samantha	Rh7AG442100 Rh7BG414600 Rh7CG463100 Rh7DG431700
rosa_wichuraiana	Rw7G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1088, 2289, 2486, 3531
AarI	CACCTGC	1 cut(s)	6695
Acc16I	TGCGCA	1 cut(s)	5498
Acc36I	ACCTGC	3 cut(s)	3928, 6273, 6695
Acc65I	GGTACC	1 cut(s)	6032
AccB1I	GGYRCC	1 cut(s)	6032
AccB7I	CCANNNNNTGG	2 cut(s)	5765, 6698
AccI	GTMKAC	2 cut(s)	2694, 3782
AccII	CGCG	2 cut(s)	181, 3381
AccIII	TCCGGA	1 cut(s)	2612
AciI	CCGC	9 cut(s)	644, 2912, 3407, 3562, 3655, 5564, 6484, 6778, 6895
AcoI	YGGCCR	1 cut(s)	4660
AcuI	CTGAAG	8 cut(s)	783, 1460, 2241, 2354, 3542, 4641, 5157, 6551
AcvI	CACGTG	1 cut(s)	6500
AdeI	CACNNNGTG	2 cut(s)	1073, 4370
AflIII	ACRYGT	6 cut(s)	482, 568, 4938, 5961, 6499, 7050
AhlI	ACTAGT	2 cut(s)	1949, 3875
AjnI	CCWGG	8 cut(s)	1227, 1659, 3822, 4840, 6028, 6338, 6381, 6649
AjuI	GAANNNNNNNTTGG	2 cut(s)	663, 695
AleI	CACNNNNGTG	1 cut(s)	4965
AloI	GAACNNNNNNTCC	2 cut(s)	6591, 6623
Alw21I	GWGCWC	7 cut(s)	621, 1464, 1924, 2091, 2721, 3805, 5758
Alw26I	GTCTC	6 cut(s)	2178, 4538, 5289, 6152, 6481, 6965
Alw44I	GTGCAC	3 cut(s)	1460, 2717, 3801
AlwNI	CAGNNNCTG	3 cut(s)	500, 1142, 6062
Ama87I	CYCGRG	1 cut(s)	2205
Aor13HI	TCCGGA	1 cut(s)	2612
ApaLI	GTGCAC	3 cut(s)	1460, 2717, 3801
Asp700I	GAANNNNTTC	9 cut(s)	1713, 2367, 2762, 5175, 6133, 6806, 6908, 6925, 6951
Asp718I	GGTACC	1 cut(s)	6032
AspLEI	GCGC	5 cut(s)	183, 384, 3500, 5499, 6690
AsuC2I	CCSGG	2 cut(s)	3767, 4675
AvaI	CYCGRG	1 cut(s)	2205
AvaII	GGWCC	6 cut(s)	2890, 3188, 3443, 4269, 6107, 6780
AxyI	CCTNAGG	1 cut(s)	949
BaeGI	GKGCMC	4 cut(s)	1464, 1795, 2721, 3805
BaeI	ACNNNNGTAYC	2 cut(s)	6299, 6332
BamHI	GGATCC	2 cut(s)	2497, 7004
BanI	GGYRCC	1 cut(s)	6032
BanII	GRGCYC	3 cut(s)	621, 4602, 5758
BauI	CACGAG	1 cut(s)	3300
BbrPI	CACGTG	1 cut(s)	6500
BbsI	GAAGAC	3 cut(s)	891, 3467, 6924
Bbv12I	GWGCWC	7 cut(s)	621, 1464, 1924, 2091, 2721, 3805, 5758
BbvCI	CCTCAGC	1 cut(s)	1629
BceAI	ACGGC	4 cut(s)	4647, 5046, 5468, 5906
BcgI	CGANNNNNNTGC	2 cut(s)	2708, 2742
BciT130I	CCWGG	8 cut(s)	1229, 1661, 3824, 4842, 6030, 6340, 6383, 6651
BciVI	GTATCC	3 cut(s)	454, 4172, 6442
BclI	TGATCA	1 cut(s)	6661
BcnI	CCSGG	2 cut(s)	3767, 4675
BcoDI	GTCTC	6 cut(s)	2178, 4538, 5289, 6152, 6481, 6965
BcuI	ACTAGT	2 cut(s)	1949, 3875
BfmI	CTRYAG	3 cut(s)	4434, 4497, 6054
BfoI	RGCGCY	2 cut(s)	3501, 6691
BfuAI	ACCTGC	3 cut(s)	3928, 6273, 6695
BfuI	GTATCC	3 cut(s)	454, 4172, 6442
BglI	GCCNNNNNGGC	1 cut(s)	4043
BglII	AGATCT	6 cut(s)	1250, 1975, 2303, 4485, 5345, 5907
BlpI	GCTNAGC	1 cut(s)	5445
BmcAI	AGTACT	2 cut(s)	1166, 1701
Bme18I	GGWCC	6 cut(s)	2890, 3188, 3443, 4269, 6107, 6780
BmeT110I	CYCGRG	1 cut(s)	2205
BmrI	ACTGGG	3 cut(s)	1992, 4597, 6588
BmuI	ACTGGG	3 cut(s)	1992, 4597, 6588
BpiI	GAAGAC	3 cut(s)	891, 3467, 6924
BplI	GAGNNNNNCTC	2 cut(s)	6934, 6966
BpmI	CTGGAG	5 cut(s)	3189, 4370, 4863, 4959, 5313
Bpu10I	CCTNAGC	2 cut(s)	1047, 1629
Bpu1102I	GCTNAGC	1 cut(s)	5445
BpuEI	CTTGAG	8 cut(s)	128, 2661, 3112, 4115, 5240, 5795, 6419, 6668
BpuMI	CCSGG	2 cut(s)	3767, 4675
BsaAI	YACGTR	2 cut(s)	2588, 6500
BsaBI	GATNNNNATC	3 cut(s)	3705, 4836, 6666
BsaI	GGTCTC	3 cut(s)	4538, 5289, 6965
BsaWI	WCCGGW	3 cut(s)	1963, 2612, 3611
Bse21I	CCTNAGG	1 cut(s)	949
Bse3DI	GCAATG	1 cut(s)	6096
Bse8I	GATNNNNATC	3 cut(s)	3705, 4836, 6666
BseAI	TCCGGA	1 cut(s)	2612
BseBI	CCWGG	8 cut(s)	1229, 1661, 3824, 4842, 6030, 6340, 6383, 6651
BseJI	GATNNNNATC	3 cut(s)	3705, 4836, 6666
BseMI	GCAATG	1 cut(s)	6096
BseRI	GAGGAG	7 cut(s)	942, 3438, 3540, 4367, 4574, 5171, 5174
BseSI	GKGCMC	4 cut(s)	1464, 1795, 2721, 3805
BseYI	CCCAGC	1 cut(s)	331
BsgI	GTGCAG	3 cut(s)	1685, 1756, 2543
Bsh1236I	CGCG	2 cut(s)	181, 3381
Bsh1285I	CGRYCG	1 cut(s)	5596
BshNI	GGYRCC	1 cut(s)	6032
BsiEI	CGRYCG	1 cut(s)	5596
BsiHKAI	GWGCWC	7 cut(s)	621, 1464, 1924, 2091, 2721, 3805, 5758
BsiHKCI	CYCGRG	1 cut(s)	2205
BsiSI	CCGG	6 cut(s)	1756, 1964, 2613, 3612, 3766, 4675
BslFI	GGGAC	7 cut(s)	973, 2761, 2903, 4582, 5270, 5333, 5939
BsmAI	GTCTC	6 cut(s)	2178, 4538, 5289, 6152, 6481, 6965
BsmFI	GGGAC	7 cut(s)	973, 2761, 2903, 4582, 5270, 5333, 5939
BsmI	GAATGC	3 cut(s)	1324, 1690, 6552
Bso31I	GGTCTC	3 cut(s)	4538, 5289, 6965
BsoBI	CYCGRG	1 cut(s)	2205
Bsp1286I	GDGCHC	9 cut(s)	621, 1464, 1795, 1924, 2091, 2721, 3805, 4602, 5758
Bsp13I	TCCGGA	1 cut(s)	2612
Bsp1407I	TGTACA	6 cut(s)	279, 607, 1414, 5339, 5835, 6064
Bsp1720I	GCTNAGC	1 cut(s)	5445
Bsp19I	CCATGG	2 cut(s)	2936, 4420
BspACI	CCGC	9 cut(s)	644, 2912, 3407, 3562, 3655, 5564, 6484, 6778, 6895
BspEI	TCCGGA	1 cut(s)	2612
BspFNI	CGCG	2 cut(s)	181, 3381
BspHI	TCATGA	1 cut(s)	3502
BspMAI	CTGCAG	3 cut(s)	4438, 4501, 6058
BspMI	ACCTGC	3 cut(s)	3928, 6273, 6695
BspQI	GCTCTTC	1 cut(s)	2868
BspT107I	GGYRCC	1 cut(s)	6032
BspTNI	GGTCTC	3 cut(s)	4538, 5289, 6965
BsrDI	GCAATG	1 cut(s)	6096
BsrGI	TGTACA	6 cut(s)	279, 607, 1414, 5339, 5835, 6064
BssNAI	GTATAC	1 cut(s)	3783
BssSI	CACGAG	1 cut(s)	3300
BssT1I	CCWWGG	6 cut(s)	2936, 3846, 4420, 6021, 6762, 6811
Bst1107I	GTATAC	1 cut(s)	3783
Bst2BI	CACGAG	1 cut(s)	3300
Bst2UI	CCWGG	8 cut(s)	1229, 1661, 3824, 4842, 6030, 6340, 6383, 6651
Bst6I	CTCTTC	9 cut(s)	1902, 2412, 2868, 3453, 4516, 5187, 6150, 6318, 6957
BstAPI	GCANNNNNTGC	1 cut(s)	6098
BstAUI	TGTACA	6 cut(s)	279, 607, 1414, 5339, 5835, 6064
BstBAI	YACGTR	2 cut(s)	2588, 6500
BstDSI	CCRYGG	3 cut(s)	2856, 2936, 4420
BstENI	CCTNNNNNAGG	2 cut(s)	3288, 6853
BstFNI	CGCG	2 cut(s)	181, 3381
BstH2I	RGCGCY	2 cut(s)	3501, 6691
BstHHI	GCGC	5 cut(s)	183, 384, 3500, 5499, 6690
BstMAI	GTCTC	6 cut(s)	2178, 4538, 5289, 6152, 6481, 6965
BstMCI	CGRYCG	1 cut(s)	5596
BstNI	CCWGG	8 cut(s)	1229, 1661, 3824, 4842, 6030, 6340, 6383, 6651
BstSFI	CTRYAG	3 cut(s)	4434, 4497, 6054
BstSLI	GKGCMC	4 cut(s)	1464, 1795, 2721, 3805
BstUI	CGCG	2 cut(s)	181, 3381
BstV2I	GAAGAC	3 cut(s)	891, 3467, 6924
BstXI	CCANNNNNNTGG	1 cut(s)	5712
BstZ17I	GTATAC	1 cut(s)	3783
Bsu36I	CCTNAGG	1 cut(s)	949
BsuI	GTATCC	3 cut(s)	454, 4172, 6442
BtgI	CCRYGG	3 cut(s)	2856, 2936, 4420
BtsI	GCAGTG	3 cut(s)	2066, 4862, 5126
BveI	ACCTGC	3 cut(s)	3928, 6273, 6695
CaiI	CAGNNNCTG	3 cut(s)	500, 1142, 6062
CciI	TCATGA	1 cut(s)	3502
CfoI	GCGC	5 cut(s)	183, 384, 3500, 5499, 6690
CseI	GACGC	2 cut(s)	3375, 6740
CspCI	CAANNNNNGTGG	4 cut(s)	1213, 1248, 2889, 2924
DraI	TTTAAA	5 cut(s)	2022, 3165, 3858, 4740, 6565
DraIII	CACNNNGTG	2 cut(s)	1073, 4370
EaeI	YGGCCR	1 cut(s)	4660
Eam1104I	CTCTTC	9 cut(s)	1902, 2412, 2868, 3453, 4516, 5187, 6150, 6318, 6957
EarI	CTCTTC	9 cut(s)	1902, 2412, 2868, 3453, 4516, 5187, 6150, 6318, 6957
EciI	GGCGGA	3 cut(s)	659, 5579, 6499
Ecl136II	GAGCTC	2 cut(s)	619, 5756
Eco130I	CCWWGG	6 cut(s)	2936, 3846, 4420, 6021, 6762, 6811
Eco147I	AGGCCT	3 cut(s)	1845, 6343, 6735
Eco24I	GRGCYC	3 cut(s)	621, 4602, 5758
Eco31I	GGTCTC	3 cut(s)	4538, 5289, 6965
Eco47I	GGWCC	6 cut(s)	2890, 3188, 3443, 4269, 6107, 6780
Eco53kI	GAGCTC	2 cut(s)	619, 5756
Eco57I	CTGAAG	8 cut(s)	783, 1460, 2241, 2354, 3542, 4641, 5157, 6551
Eco72I	CACGTG	1 cut(s)	6500
Eco81I	CCTNAGG	1 cut(s)	949
Eco88I	CYCGRG	1 cut(s)	2205
EcoICRI	GAGCTC	2 cut(s)	619, 5756
EcoNI	CCTNNNNNAGG	2 cut(s)	3288, 6853
EcoO109I	RGGNCCY	3 cut(s)	2890, 3826, 4036
EcoRI	GAATTC	1 cut(s)	200
EcoRII	CCWGG	8 cut(s)	1227, 1659, 3822, 4840, 6028, 6338, 6381, 6649
EcoT14I	CCWWGG	6 cut(s)	2936, 3846, 4420, 6021, 6762, 6811
EcoT22I	ATGCAT	2 cut(s)	2885, 3540
EcoT38I	GRGCYC	3 cut(s)	621, 4602, 5758
ErhI	CCWWGG	6 cut(s)	2936, 3846, 4420, 6021, 6762, 6811
FaqI	GGGAC	7 cut(s)	973, 2761, 2903, 4582, 5270, 5333, 5939
FauI	CCCGC	1 cut(s)	3555
FauNDI	CATATG	5 cut(s)	612, 639, 1497, 2999, 3840
FbaI	TGATCA	1 cut(s)	6661
FblI	GTMKAC	2 cut(s)	2694, 3782
FriOI	GRGCYC	3 cut(s)	621, 4602, 5758
FspAI	RTGCGCAY	1 cut(s)	5498
FspI	TGCGCA	1 cut(s)	5498
GlaI	GCGC	5 cut(s)	182, 383, 3499, 5498, 6689
GsaI	CCCAGC	1 cut(s)	335
GsuI	CTGGAG	5 cut(s)	3189, 4370, 4863, 4959, 5313
HaeII	RGCGCY	2 cut(s)	3501, 6691
HapII	CCGG	6 cut(s)	1756, 1964, 2613, 3612, 3766, 4675
HgaI	GACGC	2 cut(s)	3375, 6740
HhaI	GCGC	5 cut(s)	183, 384, 3500, 5499, 6690
Hin6I	GCGC	5 cut(s)	181, 382, 3498, 5497, 6688
HinP1I	GCGC	5 cut(s)	181, 382, 3498, 5497, 6688
HincII	GTYRAC	3 cut(s)	2043, 4504, 5076
HindII	GTYRAC	3 cut(s)	2043, 4504, 5076
HindIII	AAGCTT	7 cut(s)	1472, 1928, 2440, 3035, 3390, 4721, 5943
HpaI	GTTAAC	1 cut(s)	2043
HpaII	CCGG	6 cut(s)	1756, 1964, 2613, 3612, 3766, 4675
Hpy99I	CGWCG	2 cut(s)	1338, 6756
HpyCH4IV	ACGT	7 cut(s)	1336, 2587, 2782, 4210, 4897, 5932, 6499
HpySE526I	ACGT	7 cut(s)	1336, 2587, 2782, 4210, 4897, 5932, 6499
HspAI	GCGC	5 cut(s)	181, 382, 3498, 5497, 6688
KflI	GGGWCCC	1 cut(s)	2890
Kpn2I	TCCGGA	1 cut(s)	2612
KpnI	GGTACC	1 cut(s)	6036
Ksp22I	TGATCA	1 cut(s)	6661
KspAI	GTTAAC	1 cut(s)	2043
LguI	GCTCTTC	1 cut(s)	2868
MaeII	ACGT	7 cut(s)	1336, 2587, 2782, 4210, 4897, 5932, 6499
MfeI	CAATTG	3 cut(s)	131, 510, 4201
MhlI	GDGCHC	9 cut(s)	621, 1464, 1795, 1924, 2091, 2721, 3805, 4602, 5758
MmeI	TCCRAC	5 cut(s)	476, 1615, 3965, 4850, 6980
Mph1103I	ATGCAT	2 cut(s)	2885, 3540
MroI	TCCGGA	1 cut(s)	2612
MroXI	GAANNNNTTC	9 cut(s)	1713, 2367, 2762, 5175, 6133, 6806, 6908, 6925, 6951
MslI	CAYNNNNRTG	5 cut(s)	2688, 3402, 3987, 4965, 5710
MspI	CCGG	6 cut(s)	1756, 1964, 2613, 3612, 3766, 4675
MunI	CAATTG	3 cut(s)	131, 510, 4201
Mva1269I	GAATGC	3 cut(s)	1324, 1690, 6552
MvaI	CCWGG	8 cut(s)	1229, 1661, 3824, 4842, 6030, 6340, 6383, 6651
MvnI	CGCG	2 cut(s)	181, 3381
NciI	CCSGG	2 cut(s)	3767, 4675
NcoI	CCATGG	2 cut(s)	2936, 4420
NdeI	CATATG	5 cut(s)	612, 639, 1497, 2999, 3840
NmeAIII	GCCGAG	4 cut(s)	943, 950, 2821, 5584
NsbI	TGCGCA	1 cut(s)	5498
NsiI	ATGCAT	2 cut(s)	2885, 3540
OliI	CACNNNNGTG	1 cut(s)	4965
PaeI	GCATGC	1 cut(s)	5536
PaeR7I	CTCGAG	1 cut(s)	2205
PagI	TCATGA	1 cut(s)	3502
PaqCI	CACCTGC	1 cut(s)	6695
PceI	AGGCCT	3 cut(s)	1845, 6343, 6735
PciI	ACATGT	5 cut(s)	482, 568, 4938, 5961, 7050
PciSI	GCTCTTC	1 cut(s)	2868
PcsI	WCGNNNNNNNCGW	1 cut(s)	3580
PctI	GAATGC	3 cut(s)	1324, 1690, 6552
PdmI	GAANNNNTTC	9 cut(s)	1713, 2367, 2762, 5175, 6133, 6806, 6908, 6925, 6951
PflMI	CCANNNNNTGG	2 cut(s)	5765, 6698
PfoI	TCCNGGA	1 cut(s)	4840
PmaCI	CACGTG	1 cut(s)	6500
PmlI	CACGTG	1 cut(s)	6500
Ppu21I	YACGTR	2 cut(s)	2588, 6500
PpuMI	RGGWCCY	1 cut(s)	2890
PscI	ACATGT	5 cut(s)	482, 568, 4938, 5961, 7050
PsiI	TTATAA	4 cut(s)	1088, 2289, 2486, 3531
Psp124BI	GAGCTC	2 cut(s)	621, 5758
Psp5II	RGGWCCY	1 cut(s)	2890
Psp6I	CCWGG	8 cut(s)	1227, 1659, 3822, 4840, 6028, 6338, 6381, 6649
PspCI	CACGTG	1 cut(s)	6500
PspFI	CCCAGC	1 cut(s)	331
PspGI	CCWGG	8 cut(s)	1227, 1659, 3822, 4840, 6028, 6338, 6381, 6649
PspPPI	RGGWCCY	1 cut(s)	2890
PsrI	GAACNNNNNNTAC	6 cut(s)	1148, 1180, 3921, 3953, 6177, 6209
PstI	CTGCAG	3 cut(s)	4438, 4501, 6058
PstNI	CAGNNNCTG	3 cut(s)	500, 1142, 6062
RseI	CAYNNNNRTG	5 cut(s)	2688, 3402, 3987, 4965, 5710
SacI	GAGCTC	2 cut(s)	621, 5758
SapI	GCTCTTC	1 cut(s)	2868
ScaI	AGTACT	2 cut(s)	1166, 1701
SduI	GDGCHC	9 cut(s)	621, 1464, 1795, 1924, 2091, 2721, 3805, 4602, 5758
SfcI	CTRYAG	3 cut(s)	4434, 4497, 6054
Sfr274I	CTCGAG	1 cut(s)	2205
SinI	GGWCC	6 cut(s)	2890, 3188, 3443, 4269, 6107, 6780
SlaI	CTCGAG	1 cut(s)	2205
SmiI	ATTTAAAT	1 cut(s)	4740
SmiMI	CAYNNNNRTG	5 cut(s)	2688, 3402, 3987, 4965, 5710
SmlI	CTYRAG	9 cut(s)	107, 2205, 2676, 3127, 4094, 5219, 5810, 6434, 6683
SmoI	CTYRAG	9 cut(s)	107, 2205, 2676, 3127, 4094, 5219, 5810, 6434, 6683
SpeI	ACTAGT	2 cut(s)	1949, 3875
SphI	GCATGC	1 cut(s)	5536
SseBI	AGGCCT	3 cut(s)	1845, 6343, 6735
SsiI	CCGC	9 cut(s)	644, 2912, 3407, 3562, 3655, 5564, 6484, 6778, 6895
SspI	AATATT	5 cut(s)	469, 1674, 2551, 3007, 4246
SstI	GAGCTC	2 cut(s)	621, 5758
StuI	AGGCCT	3 cut(s)	1845, 6343, 6735
StyI	CCWWGG	6 cut(s)	2936, 3846, 4420, 6021, 6762, 6811
SwaI	ATTTAAAT	1 cut(s)	4740
TaiI	ACGT	7 cut(s)	1339, 2590, 2785, 4213, 4900, 5935, 6502
TaqI	TCGA	8 cut(s)	395, 941, 1078, 1444, 2206, 3761, 5592, 6346
TaqII	GACCGA	1 cut(s)	5610
TauI	GCSGC	2 cut(s)	2914, 6898
TspGWI	ACGGA	5 cut(s)	2050, 3015, 4452, 4462, 6902
Van91I	CCANNNNNTGG	2 cut(s)	5765, 6698
VneI	GTGCAC	3 cut(s)	1460, 2717, 3801
VpaK11BI	GGWCC	6 cut(s)	2890, 3188, 3443, 4269, 6107, 6780
XagI	CCTNNNNNAGG	2 cut(s)	3288, 6853
XbaI	TCTAGA	2 cut(s)	730, 6413
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1821, 2073, 3083, 4767
XhoI	CTCGAG	1 cut(s)	2205
XmiI	GTMKAC	2 cut(s)	2694, 3782
XmnI	GAANNNNTTC	9 cut(s)	1713, 2367, 2762, 5175, 6133, 6806, 6908, 6925, 6951
ZrmI	AGTACT	2 cut(s)	1166, 1701
Zsp2I	ATGCAT	2 cut(s)	2885, 3540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.