Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006211

Physical Location & Seq

Genomic Coordinates Reverse (-)

48042729 .. 48065910

Gene Structure 23182 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1388100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 8022 bp

ATGGCAGCTTCGCCTGTCAATTTCCTCTTCTCTTTCTTGGTTATTTGCATCACATTATGGCTCCTCTTCAGGTTGCTGGCTTGGATTTTGAGCCACGTGGTGGGAGCATCTATCAGGTTCCGGTTTGGCGGATGGAAGTGTATAAGGGATCTCGTGGTGGAGTTCAAAAAGGGCGCTGTTGAATCTGTTTCTGTCGGTGAAATTAAACTCAGTTTACGTCAGTCCTTGGTCAAACTTTTTGGTTTTATTTCTAAAGACCCTAAGCTGCAAGTGTTAATATGTGACTTAGAAGTTGTGATGAGGCCTTCGAGTAGAAGTACAGCAAAGGCTAAACCTCGAAGACCTCGTACGACAAAGGCAAATTCAGGCCGGGGAAAGTGGATGGTTGTGGCTAATATTGCAAGATATTTGTCGGTTTCCATCACAGATTTGGTTTTGAAGATGCCCAAAGCATCCATAGAAGTGAAGGAACTGAAAGTGGACATATCTAAAGATGGTGCATCCAAGCAAAATCTTATTGTTAAGCTACAGATATCGCCTATTGTTGTTCTGGGGAGTGACCCACGGGTTAGTTATGACCAGTTATCCAATTTTTCCACTGGAGGGTCCATTTCTGCAAGCCAATCATCATCTTCCATGATGGAAAGGGCATCTGCTCTTTTTATTTGTGAAGATTTCATCCTCTCTTGTGAATTTGGTCATGATAGGGAAGTTGGTGTAATGATAAAGAGTGTGGACATTGCCTGTGGAGAGATTACTGTGAACCTAAATGAGGAGATGCTTTCAAAAAGCAAAAGTTCATCACAAACTTCTTCTCAACCTGATAACGTTCTAGGGTCCACTACTGACTCTGTTGCTTCTAAAAAGCCACACAAAAAACAACAAATGATTGTTGCACTCTCAAAGTACACCTCTCTATTTCCAGAAAAGGTTTCCTTCAGTTTACCAAAACTTGACATGAGATTTGTGCATCGGGAATATGATTTTTCTGTTGAGAATAACATCATGGGCATCCAATTAAAAAGCATCAAATCACAATCTAGTGAAGATGTGGGGGAGACTACACGCCTTGATGTTCAATTGGATTTCAGTGAGATTCATTTGCTTAGAGAAGCTGGCACTTCTGTTTTGGAGATACTGAAAGTTGATGTTGTATCATTCTTTTACATTCCAGTACAGCTGACCTCGCCTGTTAGAGCTGAAGTGGATGTAAAGCTAGGAGGTACACAATGCAATGTTATAACAAATAGATTAAAGCCATGGTTGCGCCTCCACTTCTCAAAAAAGAAACGAATGGTGCTCCGGGAGGAAACTTCCACTCTTGACAAGCCACCACCAACCGATGATAACAATGCCATCATGTGGACATGTACTGTTTCAGCCCCTGAGATGACTATTGTGGTTTATAGTATCAGTGGGTTGCCATTGTATCATGGTTGCTCTCAATCATCGCACGTATTCGCGAATAACATATCAAATACAGGAACCACTGTACATATGGAACTTGGCGAGCTAAATTTGCACATGGCAGATGAATACCAAGAATGTTTGAAAGACAACCGTTTTGGCGTAGAATCAAATTCGGGTTCTATGATTAATGTTGCAAAGGTTAGTCTGGACTGGGGCAAGAAGGACATGGAACCATCTGAAGATGGTCCCAAATATAAATTGGTTTTATCTGTTGATGTGACTGGTATGGGTGTGTTTTTTACATTCAAGCGTGTCGAGTCGCTTATATCAACTGCAATGTCTTTTCAATCACTTTTGAAAACTTTGTCTTCTTCTGAAAGAAGAGCATCACATAGCCGAGGGCGTCCGTCCAAATCATCAGGGAAAGGGACTCGGCTATTGAAGTTAAATCTTGAAAGATGTTCTGTGAAATTTTGTGGTGAGGCAGGCTTAGAAAATACTGTTGTTGCAGATCCTAAGCGTGTAAACTATGGATCACAAGGTGGTCGAGTTGTAATAAGTACTTCAGATGATGGCACACCCCGGGTTGCTGGCGTAATGTCCACAACATCTGATGAACGCAAGAAATTGAGGTATTCCATTTCTCTTGACATCTTCCATTTAAGTTTGTGCGTGAATAAGGACAAACAATCCACACAGATTGAACTTGAAAGAGCCAGATCTATATATCAGGACCACTTGGAGGAAGATAAGCCGAAAACGAAAGTGGCTTTGTTTGACATCCAAAATGCCAAATTTGTACGGCGTTCTGGTGGTCTGAAAGAGGTTGCTGTGTGCTCTCTTTTCAGTGCTACTGATATTACAGTTAGGTGGGAACCTGATGTACAAATTTCACTAGTTGAACTTGGACTACAGTTGAAGTTACTTGTACATAATCAGAAGCTTCAGGGACATGTTAATGAAGATTCCCCTGGCATGAGAGGTTCTGAACAGAAAAAAGAAGTCATTCCAGAACCAGTGAATTTAGACAAGCCCAAGACGAGGGAATCTATTTTTGCTGTCGATGTGGAAATGCTGTCCATATTTGCTGAGGCTGGTGATGGGGTTGATGCAATGGTTCAGGTACAATCAATTTTTTCTGAGAATGCACGTATAGGTGTACTTCTTGAAGGGCTTATGCTTAGTTTTAATGGGTCCAGAGTATTAAAAAGTAGCCGGATGCAAATTTCACATATTCCTAGTGCTTCAAGCCCTTCTGACGCAAATACACCTGTAGCTACAACATGGGATTGGGTAATTCAAGGCCTTGATGTTCATATTAGCTTGCCATATAGGTTACAATTGCGTGCCATTGATGATTCTATTGAAGAAATGTTGCGCGCTTTGAAGCTTGTAAGTGCTGCTAGAACTAGTCTCATCTTTCCCATGAAGAAAGATACCTCAAAACCTAAGAAGCCTAGTTCAATGAAATTTGGCTGTTTAAAGTTCGGCATACGAAAGTTAACTGCTGATATTGAAGAAGAACCGCTGCAAGGTTGGCTTGATGAACACTATCGGCTGATGAAGAATGAGGCATCTGAGTTAGCTGTGAGGTTGAAGTTTCTTGATGAATTTGTTTCCAAAGCCAACCAGATTCCTAAAACCACTGAAACAGTCGATTCTACTCAAGCAAGAAAAACTTGCTTTAATGGAGTTGAGATTGATTTACAAGATCCATCAGCAGTTAGCAAGATGCAAGGAGAAATGTATAAGCAATCATTCAAATCGTATTACAAGGCATGCAAAAATCTAGCACCTTCTGAAGGTTCAGGTGCCTGTAGGGAAGGGTTTCAGGCTGGTTTTAAGCCAAGTACTGCCAGGACTTCTCTTCTTTCCATAACAGCAAGAGACTTGGATGTAACCTTGACAATGATTGATGGTGGAGATGATGGGATGATAGAGGTCGTAAAGATGCTAGATCCAGTTTGTCGTGAAAATGATATACCATTTTCTCGACTATATGGGGGTAATATTCTCTTGCAAGCAGGTTCTGTTGTCGTCCAGCTACGTGATTATACATCTCCTCTTTTGTGTGGTACTTCGGGTAAATGTGAAGGTCGTCTTGTGCTTGCACAGCAGGCAACAAGTTTTCAGCCTCAAGTATACAAAGAAGTCTATATTGGGAGATGGAGAAAGGTGAACATGCTTCGTTCGGCTAGTGGCACAACTCCACCTATGAAAACATTTACAGATTTGTCCTTACATTTTCAGAAAGCCGAAGTGTCCTTTGGGGTAGGATATGAACCTGCTTTTGCTGATGTCAGTTATGCTTTTACTGTGGCCCTTCGCAGGGCTAATCTATGTGTTAGGAACCCTAATCCACCTCCTATTCCTCCCAAAAAGGAAAAGAGCTTACCATGGTGGGATGATATGAGAAATTATATTCATGGAAATATCACCGTATTATTTTCAGAAGCCATAATTAATATACTTGCAACTACCGATCCTTATGAAAAGCTTGACAAACTTCAAACAGTAACTGGTTCTGTGGAGATTCAGCAGTCAGATGGCCGTATTTATGTATCAGCAAATGCTTTCAAGATTTTCTTAAGTAGTTTGGAGAGTTTGGCAACTAATCGTGGTTTAAAACTTCCTATTGGTGTATCTGGTCCATTGCTAGAGGCTCCAGCCTTTACAGTTGAAGTTACATTAAGTTGGGAATGTGAATCTGGAAATCCAATGAATCATTATTTGTTTGCACTTCCTGCTGAAGGAAGACCTCGTGATAAGATTTTTGATCCATACAGATCCACCTCTCTATCCCTTCGCTGGACATTTATGCTTAGGCCCTCTCCACATGAAAAGCAAGGCCCTTTTTCTACTGAGGCGGGTAATGGGGATGTAGAGGGAAATGTTTATGGTCCTCCTCATAAAAATGACAATGTTTCCATTCTTTCACCGACAATAAATGTTGGAGCTCATGATCTAGCATGGTTGATGAAGTTTGGGAACATGACTTATCTACCTCCTCACAAATTGCGGTCCTTTGCTCGTTGGCCTCGTTTTGGAGTTCCACGAATTCCTAGATCTGGTAACTTGTCATTGGACAGAGTGATGACAGAATTTATGCTCCGCATTGATGCTGCACCTACTTGTATAAAACATATGCCGCTAGATGATGATGATCCAGCGAAAGGATTGACATTCAGTATGACTAAGCTCAAATGTGAAATGTGTTATAGTCGTGGTAAGCAAAAGTTTACTTTCGAATGCAAGCGTGACCCTCTTGATCTTGTTTACCAGGGTTTTGACCTTCATATGCCTAAGGCATTTTTAAATAAAGAAGAAAGCACTAGTGTTGCAAAAGTGGTTCAAATGACAATAAAAAATTCACAATCTGCATCTATGGATAGAGTTCCCAATGAGAAAGGTAACTATGTGAGTAGTTGCACAGAGAAGCATCGTGATGATGGATTTCTATTGTCGTCTGATTATTTCACAATCAGAAGGCAGGCCCCAAAAGCTGATCCAGCGAGGTTATTAGCTTGGCAAGAGGCTGGAAAAAAAAATCTTGAGATGATGTATGTGAGGTCTGAGTTTGAAAATGGGAGTGAGAGTGATGAGCATGCACGGTCTGATCATAGTGATGATGATGGATACAATGTGGTAATAGCTGACAATTGTCAGCGCATATTTGTTTATGGGCTCAAGCTTCTGTGGAATATTGAGAATAGAGATGCTGTTTGGTCATTTGGTGGTGGTTTATCCAAAGCATTTCAGCCACCCAAACCTTCTCCTTCTAGGCAGTATGCACAAAGGAAACTACACGAGGAGAACCAGGCACATACTGATGGTGAGATGCAACAGGATGGTAGCTCAAAACCCTCTACTGCCAGCCATGGTGTTACTTCCTCTTCCATTGAGCATGCAGAGACCTCTGTTTCACTTTCATCCCCAGCACATCCCGTTAAATTTGAAAACTCATCATCAGCCACCGAGAACTCATCATCTGCTGCAGTTGGGAACTCATCATCTGTGACATCTGGTAACTCTCCATCTGTTGCAGTTGGGAACTCGTCATCTGCTATAGTTGCAAATAGTAGAGGCACAAATGATTCTGAGGAGGACGGGACTCGTCATTTCATGGTGAATGTTATTGAGCCCCAATTTAATCTTCACTCAGAAGATGCAAATGGAAGATTTCTGCTTGCTGCTGTTAGTGGCCGTGTCTTGGCTAGGTCTTTTCATTCCGTCCTTCAGGTTGGCTCTGAGATGCTCGAGCAAGCACTTGGTACTGGGAATGTAAATATTCCTGAATGTGAACCTGAAATGACATGGAAACGCATGGAGTTTTCTGTTATGTTGGAACATGTTCAAGCTCATGTTGCTCCAACAGATGTTGATCCAGGTGCTGGAGTGCAGTGGCTTCCAAAGATTCGTAGGAGTTCTCCCAAAGTGAAGCGTACTGGTGCATTACTTGAGAGAGTTTTTATGCCCTGTGATATGTACTTCCGGTACACAAGGCACAAAGGTGGAACTCCAGAGTTAAAGGTGAAGCCCTTGAAAGAGCTGACTTTCAATTCCCATAATATTACGGCAACTATGACTTCTCGCCAGTTTCAGGTTATGCTGGATGTGTTAACAAATCTTCTCTTTGCACGGCCTCCCAAGCCTAGAAAAAGCAGTCTGTCTCTTCCTGGTGAAGATGATGAAGATGTGGAGGAGGAGGCAGATGAGGTTGTTCCTGATGGTGTTGAAGAGGTAGAGCTTGCAAAAGTGGACCTTGAGCAGAAAGAGCGGGAGCGGAAATTGATTCTTGGTGACATAAGGAAACTGTCTCTTTGGTGTGATACTAATGGGGACCTATATCCAGAAAAGGAAGGTGACTTGTGGATGGTAAATTGTACAAGATCCACTCTGGTGCAAGGACTTAAAAGAGACCTTGTGAATTCCAAAAAGTCAAGGAAAGCATCATATGCATCATTAAGAATGGCTCTACATAAGGCTGCCCAGCTACGACTGATGGAAAAGGAGAAGAACAAAAGTCCTTCCTATGCTATGCGCATTTCTATGCAAATTTACAAAGTGGTTTGGAACATGCTTGTTGATGGTAAATCTTTTGCCGAGGCTGAGATCAATGACATGATTTATGACTTTGACCGGGATTACAAGGATGTTGGTGTTGCTCAGTTTACGACAAAGAATTTTGTTGTCAGAAACTGCCTGCCAAATGCCAAGTCAGATATGCTTTTATCAGCATGGAATCCTCCTCCTGAATGGGGAAAGAAAGTTCTGCTTCGTGTGGATGCAAAGCAGGGAGCTCCCAAGGACGGAAATTCTACTCTTGAACAGTTTCAGGTAGAGATCTATCCTCTCAAGATCCATTTAACCGAGACAATGTACAGAATGATGTGGGGATATTTATTCCCAGAAGAAGAGCAAGATTCACAAAGACGGCAGGAAGTTTGGAAGGTTTCAACAACTGCTGGTGCAAAACGTGTGAAGAAAGGCTCATTAATCCCTGATATTTCTGTATTAAGCAGTCTGACAAATAAAGAGTCTGAGGCCTCATCCAAATCAAGTGCTGCTCCCCCCGCTTCCAGTCAATCCTCCTCTGTTCATGCAGATCCTGTGCAAGAATCAAAATTGAAAAACCTAAAGACAACAGTTGTTGGTAGTCCAACTCGAGAGTTGAGAAGAACATCTTCCTTTGACAGATCATGGGAAGACACTGTGGCAGAATCAGTTGCTACAGAACTTGTCTTACAAAGCATTACTGGGCCACTTGGTTCCATTGAAGCAGATGAATCCTTGAAGAATAAATTGAAAGACCCCAAAGCGATCAAATCTGGCAGGTCATCCCATGAAGAAAAGAAGGTAACAAAGTCACAAGAAGAGAAAAGATCTAGGCCTCGAAAGATGATGGAGTTTCACAATATCAAGATAAGCCAGGTTGAGCTGTGTGTTACTTACGAAGGATCTAGATTTGTTGTAAATGATCTTAAGTTGCTGATGGATACGTTTCACCGTGTTGAGTTCACTGGTACTTGGCGGAGACTCTTTTCGCGGGTGAAGAAGCATATCATTTGGGGAGTCTTGAAGTCGGTGACAGGAATGCAGGGTAAGAAGTTCAAGGACAAGGCTAATAGTCAGAGGGAACCCAGTGGATCGGTTGTCCCCGACAGTGACCTTAATTTCAGCGATAATGAAGGTCAGCCAGGACAGCCTGACCAGAATCCAATAACCTTTCTCAAGCGTCCAACTGATGGAGCAGGTGATGGATTTGTCACTTCCGTTAGAGGCCTTTTCAATACTCAACGTCGCAAGGCCAAGGCATTCGTACTGCGAACCATGAGGGGTGAAGCAGAGAATGATTTCCAAGGAGATTGGAGTGAAAGTGATGTTGAGTTTTCTCCATTTGCTAGGCAGCTCACTATAACTAAAGCTAAGAGACTTATCAGGCGGCACACGAAAAAATATCGTTCAAGAAAAGGTTCATCCTCACAACAGATAGATTCTCTCCCATCCTCGCCAAGGGAGGCCACTGCATTCGAGAGTGATTCTTCTAGTGGATCCTCACCCTTCGAAGATTTTAATGAGGGAAACATTTTGTCCTCCAAAGAGGTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005575 GO:0005623 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0009267 GO:0009605 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010015 GO:0010053 GO:0010054 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0016036 GO:0016043 GO:0016049 GO:0021700 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030154 GO:0030307 GO:0030427 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035618 GO:0035619 GO:0035838 GO:0040007 GO:0040008 GO:0042594 GO:0042995 GO:0044463 GO:0044464 GO:0044706 GO:0045927 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051286 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070297 GO:0070298 GO:0071496 GO:0071695 GO:0071840 GO:0080147 GO:0090404 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0120038 GO:1902531 GO:1902532 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Apt1 Fmp27_GFWDK

Protein Analysis

2674

Amino Acids

298.04

Weight (kDa)

8.71

Isoelectric Point (pI)

46.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hobbit	PF10344	5 - 2594	0.0	Hobbit/FMP27/BTLP2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58250 AT1G58250 AT5G49680
fragaria_vesca	FvH4_5g34390
malus_domestica	MD08G1201600.v1.1 MD08G1201800.v1.1 MD08G1202400.v1.1 MD08G1208300.v1.1 MD15G1388100.v1.1
prunus_persica	Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1
pyrus_communis	pycom15g34840
rosa_chinensis	RchiOBHm_Chr7g0235041
rosa_laevigata	RLG00000001185
rosa_multiflora	Rmu_co8243721.1_g000001 Rmu_sc0002239.1_g000001 Rmu_sc0013687.1_g000001 Rmu_sc0015410.1_g000007
rosa_roxburghii	Rroxscaffold_3G00226360
rosa_rugosa	Rorug07G0287300
rosa_samantha	Rh7AG442100 Rh7BG414600 Rh7CG463100 Rh7DG431700
rosa_wichuraiana	Rw7G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1241
AarI	CACCTGC	1 cut(s)	7652
Acc16I	TGCGCA	1 cut(s)	6452
Acc36I	ACCTGC	4 cut(s)	3425, 3703, 7233, 7652
AccB1I	GGYRCC	1 cut(s)	3221
AccB7I	CCANNNNNTGG	4 cut(s)	100, 1362, 5801, 7655
AccBSI	CCGCTC	2 cut(s)	6187, 6193
AccI	GTMKAC	1 cut(s)	3552
AccII	CGCG	3 cut(s)	1463, 2790, 7456
AclI	AACGTT	1 cut(s)	828
AcoI	YGGCCR	2 cut(s)	3958, 5611
AcuI	CTGAAG	9 cut(s)	52, 922, 1221, 1668, 1959, 2339, 3231, 4179, 5630
AcvI	CACGTG	1 cut(s)	97
AcyI	GRCGYC	1 cut(s)	1813
AdeI	CACNNNGTG	2 cut(s)	100, 1952
AflII	CTTAAG	2 cut(s)	3997, 7391
AflIII	ACRYGT	4 cut(s)	1367, 2362, 5758, 6886
AhlI	ACTAGT	3 cut(s)	2305, 2819, 4742
AjnI	CCWGG	8 cut(s)	2380, 3266, 4689, 5226, 5794, 6085, 7338, 7606
AjuI	GAANNNNNNNTTGG	6 cut(s)	3552, 3584, 3660, 3692, 5547, 5579
AleI	CACNNNNGTG	2 cut(s)	5919, 6308
AloI	GAACNNNNNNTCC	2 cut(s)	6663, 6695
Alw21I	GWGCWC	4 cut(s)	1302, 2249, 4369, 6712
AlwNI	CAGNNNCTG	6 cut(s)	1385, 3440, 3929, 5801, 6609, 7019
Ama87I	CYCGRG	3 cut(s)	1992, 5666, 7074
ArsI	GACNNNNNNTTYG	8 cut(s)	947, 979, 1547, 1579, 6611, 6643, 6932, 6964
AseI	ATTAAT	3 cut(s)	1596, 3873, 6905
Asp700I	GAANNNNTTC	7 cut(s)	2415, 3237, 5760, 6741, 7093, 7763, 7882
AspLEI	GCGC	6 cut(s)	176, 1269, 2790, 2792, 5079, 6453
AsuC2I	CCSGG	5 cut(s)	371, 1304, 1993, 1994, 6551
AsuII	TTCGAA	2 cut(s)	4656, 7974
AvaI	CYCGRG	3 cut(s)	1992, 5666, 7074
AxyI	CCTNAGG	1 cut(s)	4713
BamHI	GGATCC	1 cut(s)	7961
BanI	GGYRCC	1 cut(s)	3221
BanII	GRGCYC	4 cut(s)	4369, 5097, 5553, 6712
BarI	GAAGNNNNNNTAC	2 cut(s)	331, 363
BauI	CACGAG	3 cut(s)	152, 4170, 5216
BbrPI	CACGTG	1 cut(s)	97
BbsI	GAAGAC	4 cut(s)	346, 1770, 4171, 7122
Bbv12I	GWGCWC	4 cut(s)	1302, 2249, 4369, 6712
BbvCI	CCTCAGC	1 cut(s)	2499
BceAI	ACGGC	6 cut(s)	2228, 3945, 5598, 6000, 6065, 6860
BciT130I	CCWGG	8 cut(s)	2382, 3268, 4691, 5228, 5796, 6087, 7340, 7608
BciVI	GTATCC	2 cut(s)	5039, 7399
BclI	TGATCA	1 cut(s)	5026
BcnI	CCSGG	5 cut(s)	371, 1304, 1993, 1994, 6551
BcuI	ACTAGT	3 cut(s)	2305, 2819, 4742
BfmI	CTRYAG	7 cut(s)	527, 2321, 2682, 3226, 5403, 5475, 7140
BfoI	RGCGCY	1 cut(s)	177
BfrI	CTTAAG	2 cut(s)	3997, 7391
BfuAI	ACCTGC	4 cut(s)	3425, 3703, 7233, 7652
BfuI	GTATCC	2 cut(s)	5039, 7399
BglII	AGATCT	4 cut(s)	2129, 4475, 6753, 7292
BmcAI	AGTACT	2 cut(s)	1972, 3262
BmeT110I	CYCGRG	3 cut(s)	1992, 5666, 7074
BmrI	ACTGGG	4 cut(s)	1630, 5694, 7176, 7545
BmuI	ACTGGG	4 cut(s)	1630, 5694, 7176, 7545
BoxI	GACNNNNGTC	1 cut(s)	5070
BpiI	GAAGAC	4 cut(s)	346, 1770, 4171, 7122
BpmI	CTGGAG	4 cut(s)	621, 4059, 5823, 5913
Bpu10I	CCTNAGC	4 cut(s)	261, 1926, 2499, 4232
Bpu14I	TTCGAA	2 cut(s)	4656, 7974
BpuEI	CTTGAG	8 cut(s)	3060, 3531, 4982, 5081, 5888, 6194, 6749, 7625
BpuMI	CCSGG	5 cut(s)	371, 1304, 1993, 1994, 6551
BsaAI	YACGTR	4 cut(s)	97, 1456, 2561, 3458
BsaBI	GATNNNNATC	2 cut(s)	4572, 5790
BsaHI	GRCGYC	1 cut(s)	1813
BsaI	GGTCTC	2 cut(s)	5315, 6321
BsaWI	WCCGGW	2 cut(s)	120, 5901
BsaXI	ACNNNNNCTCC	4 cut(s)	4990, 5020, 7771, 7801
Bse21I	CCTNAGG	1 cut(s)	4713
Bse3DI	GCAATG	5 cut(s)	738, 1240, 1752, 2529, 4061
Bse8I	GATNNNNATC	2 cut(s)	4572, 5790
BseBI	CCWGG	8 cut(s)	2382, 3268, 4691, 5228, 5796, 6087, 7340, 7608
BseJI	GATNNNNATC	2 cut(s)	4572, 5790
BseMI	GCAATG	5 cut(s)	738, 1240, 1752, 2529, 4061
BsePI	GCGCGC	1 cut(s)	2788
BseYI	CCCAGC	2 cut(s)	5344, 6399
BsgI	GTGCAG	2 cut(s)	4518, 5828
Bsh1236I	CGCG	3 cut(s)	1463, 2790, 7456
BshNI	GGYRCC	1 cut(s)	3221
BsiHKAI	GWGCWC	4 cut(s)	1302, 2249, 4369, 6712
BsiHKCI	CYCGRG	3 cut(s)	1992, 5666, 7074
BsiSI	CCGG	7 cut(s)	121, 370, 1303, 1993, 2626, 5902, 6550
BsiWI	CGTACG	1 cut(s)	347
BslFI	GGGAC	6 cut(s)	1641, 1852, 2373, 5533, 6263, 7550
BsmFI	GGGAC	6 cut(s)	1641, 1852, 2373, 5533, 6263, 7550
BsmI	GAATGC	5 cut(s)	2560, 4664, 7509, 7724, 7937
Bso31I	GGTCTC	2 cut(s)	5315, 6321
BsoBI	CYCGRG	3 cut(s)	1992, 5666, 7074
Bsp119I	TTCGAA	2 cut(s)	4656, 7974
Bsp1286I	GDGCHC	6 cut(s)	1302, 2249, 4369, 5097, 5553, 6712
Bsp1407I	TGTACA	5 cut(s)	1492, 2293, 2338, 6293, 6789
Bsp19I	CCATGG	3 cut(s)	1259, 3806, 5287
Bsp68I	TCGCGA	1 cut(s)	1463
BspFNI	CGCG	3 cut(s)	1463, 2790, 7456
BspHI	TCATGA	2 cut(s)	700, 4369
BspMAI	CTGCAG	1 cut(s)	5407
BspMI	ACCTGC	4 cut(s)	3425, 3703, 7233, 7652
BspQI	GCTCTTC	2 cut(s)	1786, 6819
BspT104I	TTCGAA	2 cut(s)	4656, 7974
BspT107I	GGYRCC	1 cut(s)	3221
BspTI	CTTAAG	2 cut(s)	3997, 7391
BspTNI	GGTCTC	2 cut(s)	5315, 6321
BsrBI	CCGCTC	2 cut(s)	6187, 6193
BsrDI	GCAATG	5 cut(s)	738, 1240, 1752, 2529, 4061
BsrGI	TGTACA	5 cut(s)	1492, 2293, 2338, 6293, 6789
BssHII	GCGCGC	1 cut(s)	2788
BssNAI	GTATAC	1 cut(s)	3553
BssNI	GRCGYC	1 cut(s)	1813
BssSI	CACGAG	3 cut(s)	152, 4170, 5216
BssT1I	CCWWGG	8 cut(s)	225, 1259, 3806, 5287, 6714, 7719, 7768, 7922
Bst1107I	GTATAC	1 cut(s)	3553
Bst2BI	CACGAG	3 cut(s)	152, 4170, 5216
Bst2UI	CCWGG	8 cut(s)	2382, 3268, 4691, 5228, 5796, 6087, 7340, 7608
Bst6I	CTCTTC	9 cut(s)	32, 71, 1786, 3282, 5308, 6087, 6141, 6819, 7278
BstACI	GRCGYC	1 cut(s)	1813
BstAFI	CTTAAG	2 cut(s)	3997, 7391
BstAPI	GCANNNNNTGC	2 cut(s)	4154, 6365
BstAUI	TGTACA	5 cut(s)	1492, 2293, 2338, 6293, 6789
BstBAI	YACGTR	4 cut(s)	97, 1456, 2561, 3458
BstBI	TTCGAA	2 cut(s)	4656, 7974
BstDSI	CCRYGG	4 cut(s)	563, 1259, 3806, 5287
BstENI	CCTNNNNNAGG	4 cut(s)	770, 3210, 4158, 7921
BstFNI	CGCG	3 cut(s)	1463, 2790, 7456
BstH2I	RGCGCY	1 cut(s)	177
BstHHI	GCGC	6 cut(s)	176, 1269, 2790, 2792, 5079, 6453
BstNI	CCWGG	8 cut(s)	2382, 3268, 4691, 5228, 5796, 6087, 7340, 7608
BstNSI	RCATGY	8 cut(s)	1371, 2366, 3192, 3595, 5018, 5318, 5762, 6490
BstPAI	GACNNNNGTC	1 cut(s)	5070
BstSFI	CTRYAG	7 cut(s)	527, 2321, 2682, 3226, 5403, 5475, 7140
BstUI	CGCG	3 cut(s)	1463, 2790, 7456
BstV2I	GAAGAC	4 cut(s)	346, 1770, 4171, 7122
BstZ17I	GTATAC	1 cut(s)	3553
Bsu36I	CCTNAGG	1 cut(s)	4713
BsuI	GTATCC	2 cut(s)	5039, 7399
BtgI	CCRYGG	4 cut(s)	563, 1259, 3806, 5287
BtgZI	GCGATG	1 cut(s)	1434
BtsI	GCAGTG	2 cut(s)	5816, 7932
BtuMI	TCGCGA	1 cut(s)	1463
BveI	ACCTGC	4 cut(s)	3425, 3703, 7233, 7652
CaiI	CAGNNNCTG	6 cut(s)	1385, 3440, 3929, 5801, 6609, 7019
CciI	TCATGA	2 cut(s)	700, 4369
CfoI	GCGC	6 cut(s)	176, 1269, 2790, 2792, 5079, 6453
Cfr9I	CCCGGG	1 cut(s)	1992
CseI	GACGC	3 cut(s)	1802, 2678, 7634
CspCI	CAANNNNNGTGG	2 cut(s)	2092, 2127
DraI	TTTAAA	3 cut(s)	2892, 4035, 4725
DraIII	CACNNNGTG	2 cut(s)	100, 1952
EaeI	YGGCCR	2 cut(s)	3958, 5611
Eam1104I	CTCTTC	9 cut(s)	32, 71, 1786, 3282, 5308, 6087, 6141, 6819, 7278
EarI	CTCTTC	9 cut(s)	32, 71, 1786, 3282, 5308, 6087, 6141, 6819, 7278
EciI	GGCGGA	2 cut(s)	144, 7456
Ecl136II	GAGCTC	2 cut(s)	4367, 6710
Eco130I	CCWWGG	8 cut(s)	225, 1259, 3806, 5287, 6714, 7719, 7768, 7922
Eco147I	AGGCCT	5 cut(s)	304, 2715, 6956, 7300, 7692
Eco24I	GRGCYC	4 cut(s)	4369, 5097, 5553, 6712
Eco31I	GGTCTC	2 cut(s)	5315, 6321
Eco32I	GATATC	1 cut(s)	534
Eco53kI	GAGCTC	2 cut(s)	4367, 6710
Eco57I	CTGAAG	9 cut(s)	52, 922, 1221, 1668, 1959, 2339, 3231, 4179, 5630
Eco72I	CACGTG	1 cut(s)	97
Eco81I	CCTNAGG	1 cut(s)	4713
Eco88I	CYCGRG	3 cut(s)	1992, 5666, 7074
EcoICRI	GAGCTC	2 cut(s)	4367, 6710
EcoNI	CCTNNNNNAGG	4 cut(s)	770, 3210, 4158, 7921
EcoO109I	RGGNCCY	4 cut(s)	4236, 4259, 4903, 6250
EcoRI	GAATTC	2 cut(s)	4467, 6337
EcoRII	CCWGG	8 cut(s)	2380, 3266, 4689, 5226, 5794, 6085, 7338, 7606
EcoRV	GATATC	1 cut(s)	534
EcoT14I	CCWWGG	8 cut(s)	225, 1259, 3806, 5287, 6714, 7719, 7768, 7922
EcoT22I	ATGCAT	1 cut(s)	6370
EcoT38I	GRGCYC	4 cut(s)	4369, 5097, 5553, 6712
ErhI	CCWWGG	8 cut(s)	225, 1259, 3806, 5287, 6714, 7719, 7768, 7922
FaqI	GGGAC	6 cut(s)	1641, 1852, 2373, 5533, 6263, 7550
FauI	CCCGC	4 cut(s)	4270, 6180, 6991, 7449
FauNDI	CATATG	4 cut(s)	1497, 4554, 4707, 6364
FbaI	TGATCA	1 cut(s)	5026
FblI	GTMKAC	1 cut(s)	3552
FriOI	GRGCYC	4 cut(s)	4369, 5097, 5553, 6712
FspAI	RTGCGCAY	1 cut(s)	6452
FspI	TGCGCA	1 cut(s)	6452
GlaI	GCGC	6 cut(s)	175, 1268, 2789, 2791, 5078, 6452
GsaI	CCCAGC	2 cut(s)	5348, 6403
GsuI	CTGGAG	4 cut(s)	621, 4059, 5823, 5913
HaeII	RGCGCY	1 cut(s)	177
HapII	CCGG	7 cut(s)	121, 370, 1303, 1993, 2626, 5902, 6550
HgaI	GACGC	3 cut(s)	1802, 2678, 7634
HhaI	GCGC	6 cut(s)	176, 1269, 2790, 2792, 5079, 6453
Hin1I	GRCGYC	1 cut(s)	1813
Hin6I	GCGC	6 cut(s)	174, 1267, 2788, 2790, 5077, 6451
HinP1I	GCGC	6 cut(s)	174, 1267, 2788, 2790, 5077, 6451
HincII	GTYRAC	2 cut(s)	2913, 6030
HindII	GTYRAC	2 cut(s)	2913, 6030
HindIII	AAGCTT	4 cut(s)	2351, 2798, 3905, 5099
HpaI	GTTAAC	2 cut(s)	2913, 6030
HpaII	CCGG	7 cut(s)	121, 370, 1303, 1993, 2626, 5902, 6550
Hpy99I	CGWCG	1 cut(s)	7713
HpyCH4IV	ACGT	9 cut(s)	96, 217, 828, 1455, 2560, 3457, 6886, 7409, 7708
HpySE526I	ACGT	9 cut(s)	96, 217, 828, 1455, 2560, 3457, 6886, 7409, 7708
Hsp92I	GRCGYC	1 cut(s)	1813
HspAI	GCGC	6 cut(s)	174, 1267, 2788, 2790, 5077, 6451
Ksp22I	TGATCA	1 cut(s)	5026
KspAI	GTTAAC	2 cut(s)	2913, 6030
LguI	GCTCTTC	2 cut(s)	1786, 6819
MaeII	ACGT	9 cut(s)	96, 217, 828, 1455, 2560, 3457, 6886, 7409, 7708
MbiI	CCGCTC	2 cut(s)	6187, 6193
MfeI	CAATTG	3 cut(s)	1079, 2750, 5068
MhlI	GDGCHC	6 cut(s)	1302, 2249, 4369, 5097, 5553, 6712
MlyI	GAGTC	7 cut(s)	842, 1736, 1834, 5515, 6956, 7440, 7491
MmeI	TCCRAC	5 cut(s)	4342, 5733, 5804, 7094, 7673
Mph1103I	ATGCAT	1 cut(s)	6370
MroXI	GAANNNNTTC	7 cut(s)	2415, 3237, 5760, 6741, 7093, 7763, 7882
MslI	CAYNNNNRTG	9 cut(s)	461, 2367, 4323, 4854, 5034, 5238, 5919, 6308, 6458
MspA1I	CMGCKG	2 cut(s)	1180, 2938
MspCI	CTTAAG	2 cut(s)	3997, 7391
MspI	CCGG	7 cut(s)	121, 370, 1303, 1993, 2626, 5902, 6550
MunI	CAATTG	3 cut(s)	1079, 2750, 5068
Mva1269I	GAATGC	5 cut(s)	2560, 4664, 7509, 7724, 7937
MvaI	CCWGG	8 cut(s)	2382, 3268, 4691, 5228, 5796, 6087, 7340, 7608
MvnI	CGCG	3 cut(s)	1463, 2790, 7456
NciI	CCSGG	5 cut(s)	371, 1304, 1993, 1994, 6551
NcoI	CCATGG	3 cut(s)	1259, 3806, 5287
NdeI	CATATG	4 cut(s)	1497, 4554, 4707, 6364
NmeAIII	GCCGAG	3 cut(s)	1822, 1832, 6538
NruI	TCGCGA	1 cut(s)	1463
NsbI	TGCGCA	1 cut(s)	6452
NsiI	ATGCAT	1 cut(s)	6370
NspI	RCATGY	8 cut(s)	1371, 2366, 3192, 3595, 5018, 5318, 5762, 6490
NspV	TTCGAA	2 cut(s)	4656, 7974
OliI	CACNNNNGTG	2 cut(s)	5919, 6308
PaeI	GCATGC	3 cut(s)	3192, 5018, 5318
PaeR7I	CTCGAG	2 cut(s)	5666, 7074
PagI	TCATGA	2 cut(s)	700, 4369
PaqCI	CACCTGC	1 cut(s)	7652
PauI	GCGCGC	1 cut(s)	2788
PceI	AGGCCT	5 cut(s)	304, 2715, 6956, 7300, 7692
PciI	ACATGT	3 cut(s)	1367, 2362, 5758
PciSI	GCTCTTC	2 cut(s)	1786, 6819
PcsI	WCGNNNNNNNCGW	2 cut(s)	343, 4447
PctI	GAATGC	5 cut(s)	2560, 4664, 7509, 7724, 7937
PdmI	GAANNNNTTC	7 cut(s)	2415, 3237, 5760, 6741, 7093, 7763, 7882
Pfl23II	CGTACG	1 cut(s)	347
PflMI	CCANNNNNTGG	4 cut(s)	100, 1362, 5801, 7655
PfoI	TCCNGGA	1 cut(s)	1302
PleI	GAGTC	7 cut(s)	842, 1735, 1834, 5515, 6955, 7440, 7490
PmaCI	CACGTG	1 cut(s)	97
PmlI	CACGTG	1 cut(s)	97
PpsI	GAGTC	7 cut(s)	842, 1735, 1834, 5515, 6955, 7440, 7490
Ppu21I	YACGTR	4 cut(s)	97, 1456, 2561, 3458
PpuMI	RGGWCCY	1 cut(s)	6250
PscI	ACATGT	3 cut(s)	1367, 2362, 5758
PshAI	GACNNNNGTC	1 cut(s)	5070
PshBI	ATTAAT	3 cut(s)	1596, 3873, 6905
PsiI	TTATAA	1 cut(s)	1241
Psp124BI	GAGCTC	2 cut(s)	4369, 6712
Psp1406I	AACGTT	1 cut(s)	828
Psp5II	RGGWCCY	1 cut(s)	6250
Psp6I	CCWGG	8 cut(s)	2380, 3266, 4689, 5226, 5794, 6085, 7338, 7606
PspCI	CACGTG	1 cut(s)	97
PspFI	CCCAGC	2 cut(s)	5344, 6399
PspGI	CCWGG	8 cut(s)	2380, 3266, 4689, 5226, 5794, 6085, 7338, 7606
PspLI	CGTACG	1 cut(s)	347
PspPPI	RGGWCCY	1 cut(s)	6250
PspXI	VCTCGAGB	1 cut(s)	5666
PsrI	GAACNNNNNNTAC	6 cut(s)	2277, 2304, 2309, 2336, 7137, 7169
PstI	CTGCAG	1 cut(s)	5407
PstNI	CAGNNNCTG	6 cut(s)	1385, 3440, 3929, 5801, 6609, 7019
PteI	GCGCGC	1 cut(s)	2788
PvuII	CAGCTG	1 cut(s)	1180
RruI	TCGCGA	1 cut(s)	1463
RseI	CAYNNNNRTG	9 cut(s)	461, 2367, 4323, 4854, 5034, 5238, 5919, 6308, 6458
SacI	GAGCTC	2 cut(s)	4369, 6712
SapI	GCTCTTC	2 cut(s)	1786, 6819
ScaI	AGTACT	2 cut(s)	1972, 3262
SchI	GAGTC	7 cut(s)	842, 1736, 1834, 5515, 6956, 7440, 7491
SduI	GDGCHC	6 cut(s)	1302, 2249, 4369, 5097, 5553, 6712
SfcI	CTRYAG	7 cut(s)	527, 2321, 2682, 3226, 5403, 5475, 7140
Sfr274I	CTCGAG	2 cut(s)	5666, 7074
SfuI	TTCGAA	2 cut(s)	4656, 7974
SlaI	CTCGAG	2 cut(s)	5666, 7074
SmaI	CCCGGG	1 cut(s)	1994
SmiMI	CAYNNNNRTG	9 cut(s)	461, 2367, 4323, 4854, 5034, 5238, 5919, 6308, 6458
SpeI	ACTAGT	3 cut(s)	2305, 2819, 4742
SphI	GCATGC	3 cut(s)	3192, 5018, 5318
SseBI	AGGCCT	5 cut(s)	304, 2715, 6956, 7300, 7692
SspI	AATATT	5 cut(s)	397, 3421, 5113, 5698, 5980
SstI	GAGCTC	2 cut(s)	4369, 6712
StuI	AGGCCT	5 cut(s)	304, 2715, 6956, 7300, 7692
StyI	CCWWGG	8 cut(s)	225, 1259, 3806, 5287, 6714, 7719, 7768, 7922
TaiI	ACGT	9 cut(s)	99, 220, 831, 1458, 2563, 3460, 6889, 7412, 7711
TauI	GCSGC	2 cut(s)	4561, 7855
TspGWI	ACGGA	4 cut(s)	1806, 5629, 6735, 7672
TspMI	CCCGGG	1 cut(s)	1992
Van91I	CCANNNNNTGG	4 cut(s)	100, 1362, 5801, 7655
Vha464I	CTTAAG	2 cut(s)	3997, 7391
VspI	ATTAAT	3 cut(s)	1596, 3873, 6905
XagI	CCTNNNNNAGG	4 cut(s)	770, 3210, 4158, 7921
XbaI	TCTAGA	1 cut(s)	7370
XceI	RCATGY	8 cut(s)	1371, 2366, 3192, 3595, 5018, 5318, 5762, 6490
XcmI	CCANNNNNNNNNTGG	3 cut(s)	427, 1495, 1666
XhoI	CTCGAG	2 cut(s)	5666, 7074
XmaI	CCCGGG	1 cut(s)	1992
XmiI	GTMKAC	1 cut(s)	3552
XmnI	GAANNNNTTC	7 cut(s)	2415, 3237, 5760, 6741, 7093, 7763, 7882
ZrmI	AGTACT	2 cut(s)	1972, 3262
Zsp2I	ATGCAT	1 cut(s)	6370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.