Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006211

Physical Location & Seq

Genomic Coordinates Reverse (-)

26146874 .. 26171179

Gene Structure 24306 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1201800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 8031 bp

ATGGCGGCTTCGCCCGTCAATTTCCTCTTCTGTTTCTTGGTCATTTGCATCACGTTATGGCTCCTCTTCATATTTTCTTCAAGGTTGCTGGCTTGGATTTTGAGCCGCGTAGTGGGAGCATCTATCAGATTCCGGTTTGGCGGATGGAAGTGTATAAGGGATCTCGTGGTGGAGTTCAAAAAGGGCGCTGTTGAATCTGTTTCTGTTGGTGAAATTAAACTCAGCTTACGTCAGTCCTTGGTCAAACTGTTTGGTTTTATTTCTAAAGACCCTAAGCTGCAAGTGTTAATATGTGACTTAGAAGTTGTGATGAGGCCTTCGAGTAGAAGTACAGCAAAGGCTAAACCTCGAAGACCTCGTACGACAAAGGCCAATTCAGGCAGGGGAAAGTGGATGGTTGTGGCTAATATTGCAAGATATTTGTCGGTTTCCATCACAGATTTGGTTTTGAAGATGCCCAAAGCATCCATAGAAGTGAAGGAACTGAAAGTGGACATATCTAAAGATGGTGCATCCAAGCAAAATCTTATTGTTAAGCTACAGATATCACCTATTGTTGTTCTGAGGAGTGACCCACGGGTTAGTTGTGACTTATCCAATTTTTCCACTGGAGGATCCATTTCTGCAAGCCAGACATCATCCTCCATGATGGAAAGGACATCTGCTCTTTTTATTTGTGAAGATTTCATCCTCTCTTGTGAATTTGGTCATGATAGGGAAGTCGGTGTAATGATCAAGAATGTGGACATTGCCTGTGGAGAGATTACTGTGAACCTAAATGAGGAGTTGCTTTCAAAAAGCAAAAGTTCATCACAAACTTCTTTTCAACCTGATAACGTTCTAGGGTCCTCTACTGATTCTGTTGCTTCTAAAAAGCCACACAAAAAACAACAAATGATTGTTGCACTCTCAAAGTACACCTCTCTATTTCCAGAAAAGGTTTCCTTCAGTTTACCAAAACTGGACGTGAGATTTGTGCATCGGGAATATGATTTTTCTGTCGAGAATAACATAATGGGTATCCAATTAAAAAGCACCAAATCACAATCCAGTGAAGATGTGGGGGAGACTACACGCCTTGATGTTCAATTGGATTTCAGTGAGATTCATTTGCTTAGAGAAGCTGGCATTTCTGTTTTGGAGATACTGAAAGTTGATGTTGCATCATTCTTTTACATTCCAGTACAGCCAACCTTGCCTGTCAGAGCTGAAATGGATGTAAAGCTTGGAGGTACACAATGCAACGTTATAATGAATAGATTAAAGCCATGGTTGCGCCTCCACTTCTCAAAAAAGAAACGAATGGTGCTTCGGGAGGGAACTTCCACTCTTGACAAGCCACCGCCAACCGATGATACCAAAGCCATCATGTGGACATGTACTGTTTCAGCCCCTGAGATGACTATTGTGGTTTATAGCATCAGTGGGTTGCCATTGTATCATGGTTGCTCTCAATCATCGCACATATTTGCGAATAACATATCAAATACGGGAACCACTGTACATATGGAACTTGGTGAGCTAAATTTGCACATGGCAGATGAATACCAAGAATGCTTGAAAGAGAGCCTTTTTGGTGTAGAATCAAATTCGGGTTCTATAATTAATGTTGGAAAGGTTAGTCTGGACTGGGGCAAAAAGGACATGGAATCATCCAAAGAAGACGGTCCGAAATGTAAATTGGTTTTATCTGTTGATGTGACTGGTATGGGTGTGTTTTTTACATTCAAGCGTGTTGAGTCGCTTATATCAACTGCAATGTCCTTTCAAGCACTTTTGAAAACTTTGTCTTCTTCTGAAAGAAGAGCATCACAAAGCCGAGGGCGTCCGTCCAAACCATCAGGGAAAGGGACTCAGCTGTTGAAGTTAAATCTTGAACGATGTTCTGTGAAATTTTGTGGTGAGGCAGGCTTAGAAAATACTGTTGTTGCAGATCCTAAGCGTGTGAACTATGGATCACAAGGTGGTCGAGTTGTAATAAGTACCTCAGATGATGGCACACCCCGGGTTGCAGACATAATGTCCACAACATCTGATGAACGCAAGAAACTGAGGTATTCCATTTCTCTTGACATCTTCCATTTAAGTTTGCGCGTGAACAAGGAGAAACAATCCACACAGATTGAACTTGAAAGAGCCAGATCTATCTATCAGGACCACTTGGAGGAAGATAAACCGGAAACGAAAGTGGCTTTGTTTGACATCCAGAATTCCAAATTTGTACGGCGTTCTGGTGGTCTGAAAGAGGTTGCTGTTTGCTCTCTTTTCAGTGCTACTGATATTACAGTTAGGTGGGAACCTGATGTACAATTATCACTAGTTGAACTTGGACTACAGTTGAAGTTACTTGTACATAATCAGAAGCTTCAGAGACATGGTAGTGAAGATGCCCCTGGCATGAAAGGTTCTGACCAGAAAAAAGAAGTCATTGCAGAACCAGTGAATTTAGACAAGCCCAAGAAGAGGGAATCTATTTTTGCTGTAGATGTGGAAATGCTGTCCATATTTGCTGAGGCTGGTGATGGGGTTGATGCAATGGTTCAGGTACAATCAATTTTTTCTGAGAATGCCCGTATAGGTGTACTTCTTGAAGGGCTTATGCTTAGTTTTAATGGGTCCAGAGTATTAAAAAGTAGCCGGATGCAAATTTCACGTGTTCCTAGTGCCTCATGCCCTTCCGACGCAAAAACACCTGTAGCTACAACATGGGATTGGGTAATTCAAGGCCTTGATGTTCATATTTGCTTGCCATATAGGTTAGAATTGCGCGCCATTGATGATTCTGTTGAAGAAATGTTGCGTGCTTTGAAGCTTGTAAGTGCTGCTAGAACTAGCCTCATCTTTCCCACGAAGAAAGATACCTCAAAACCTAAGAAGCCTGGTTCAATGAAAGTTGGCTGTTTAAAGTTTGGCATACGAAAGTTAACTGCTGATATTGAAGAAGAGCCGCTGCAAGGTTGGCTTGATGAACATTATCAGCTGATGAAGAATGAGGCATCTGAGTTAGCTGTTAGGTTGAAGTTTCTTGATGAATTTGTTTCCAAAGCCAACCAGATTCCTAAAACAACCGAAACAGTAGATTCTACTCAAGGAAGAAAAACTTTCTTTAATGGAGTTGAGATTGATGTACAAGATCCATCAGCAGTTAATGAGATGAAAGGAGAAATTTATAAACAATCATTCAAATCTTATTACAGGGCATGCAAAAATCTAGCACCATCTGAAGGTTCAGGTGCCTGTAGGGAAGGGTTTCAGTCTGGTTTTAAGCCAAGTACTTCTAGGACTTCTCTTCTTTCCATAACAGCAAGAGACTTAGATGTAACCTTGACAAGGATTGATGGTGGAGATGATGGGATGATAGAGGTCATAAAGACGCTAGATCCGGTTTGTTGTGAAAATGATATACCATTTTCTCGACTATATGGGAGTAATATTCTCTTGCACGCAGGTTCTCTGGTCGTTCAGCTACGTGATTATGCATCTCCTCTTTTGTGTGGTACTTCTGGTAAATGTGAAGGTCGTCTTGTACTTGCACAGCAGGCAACAAGTTTTCAGCCTCAAGTATACAAAGAAGTCTATATTGGGAGATGGAGAAAGGTGAACATGCTTCGTTCGGCTAGTGGCACAACTCCACCTATGAAAACATTTACAGATTTATCCTTACATTTTCAGAAAGCTGAAGTGTCCTTTGGGGTGGGGTATGAACCTGCTTTTGCTGATGTCACTTATGCTTTTTCTGTGGCCCTTCGCAGGGCTAATCTATCTGTTAGGAACCCTAATCCACTTCCCACTCCTCCCAAAAAGGAAAAGAGCTTACCATGGTGGGATGATATGAGAAATTATATTCATGGAAACATCACCTTATTATTTTCAGAAGCCAAATTTAATATACTAGCGACTACCGATCCTTATGAAAAGCTTGACAAACTTCAAATAATAACTGGTTCTATGGAGATTCAGCAGTCTGATGGCCGTATTTATGTATCAGCAAATGCTTTCAAGATTTTCTTAAGCAGTTTGGAGAGTTTGGCAAATAATCGTGGTTTAAAACTTCCTAAAGGTGTATCCGGTCCATTGCTAGAGGCTCCAGCCTTTACAGTTGAAGTTACATTAGGTTGGGAATGTGAATCTGGAAATCCCATGAATCATTATTTGTTTGCACTTCCTGCTGAAGGAAGACCTCGTGATAAGGTTTTTGATCCATACAGATCCACCTCTCTATCCCTTCGCTGGACCTTTTCTCTTAGGCCCTCTCCACTTGAAAAGCAAAGCACTTTTTCTACCGAGGCAGGTAATGGTGATGTAGATGGAACTGTTTATGGTCCTCCTCATAAAGATGACAATGTTTCCGTTCTTTCACCGACACTAAATGTTGGCGCTCATGATCTAGCATGGTTGATAAAGTTTTGGAACATGAATTATCTTCCTCCTCACAAATTGCGGTCCTTTGCTCGTTGGCCTCGTTTTGGAGTTCCAAGAGTTCCTAGATCTGGTAACTTGTCATTGGACAGAGTAATGACAGAATTTATGCTCCGCATTGATGCTGCACCTACATGTTTAAAACATATGCCGCTAGATGATGATGATCCAGCTAAGGGACTGACATTTAATATGACTAAGCTCAAATGTGAAATGTGTTATAGTCGTGGTAAGCAAAAGTATACTTTTGAATGCAAGCGTGAGCCTCTTGATCTTGTTTACCAGGGTTTTGACCTTCATATGCCTAAGGCATTTTTAAATAAAAAAGAAAGTACTAGTGTTGCAAAAGTGGTTCAAATGACAATAAAAAATTCACAATCTGCATCTACGGATAGAGGTCCCAATGAGAAAAGTAACTATGTGAGTAGTTGCACAGAGAAGCATCGTGATGATGGATTTCTATTGTCATCTGATTATTTCACAATAAGAAGGCAGGCGCCAAAAGCTGACCCAGCCAGGTTATTAGCTTGGCAAGAGGCTGGAAGAAAAAATCTTGAGATGACATATGTGAGGTCTGAGTTTGAAAATGGGAGTGAGAGTGATGAGCATACACGGTCTGATCATAGTGATGATGATGGATACAATGTGGTAATAGCTGACAATTGTCAGCGTATATTTGTTTATGGGCTAAAGCTTCTGTGGACTATTGAGAATAGAGATGCTGTTTGGTCTTTTGTTGGTGGTTTATCCAAAGCATTTCAGCCATCCAAACCTTCTCCTTCTAGGCAGTATGCCCAAAGGAAATTACACGAGGAGAACCAGGCACATACTGGTGGTGAAATGCAACAAGATGGTAACTCTAAACCCTCTACTACCAGCCATGGTGTTACTTCCTCTTCCGTCGAGCATGCAGAGACCTCTGGTTCACTTTCATCTCCAGCACATCCCGTTAAATTAGAAAACTCATCATCAGCCACCGAGAACTCATCATCTGTTGCAGTTGGGAACTCATCATCAGCGACATCTGGTAACTCTCCATCTGTTGTGGTTGGGAACTCGTCATCTGCTGTAGTTGCAAAGAATAGAGACACAAATGATTCTGAGGAGGAGGGGACTCGTCATTTCATGGTGAATGTTATTGAGCCCCAATTTAATCTTCACTCAGAAGATGCAAATGGAAGATTTCTGCTTGCTGCTGTTAGTGGCCGGGTCTTGGCTAGGTCTTTTCATTCAGTCCTTCAGGTTGGCTCTGAGATGCTCGAGCAAGCACTTGGTACTGGGAATGTAAATATTCCAGAATGTGAACCTGAAATGACATGGAAACGCATGGAGTTTTCTGTTATGTTGGAACATGTTCAAGCTCATGTTGCGCCAACAGATGTTGATCCAGGTGCTGGATTGCAGTGGCTTCCAAAGATTCGTAGAAGTTCTCCAAAAGTTAAACGTACTGGTGCATTACTTGAGAGAGTTTTTATGCCCTGCGATATGTACTTCCGGTACACAAGGCACAAAGGTGGAACTCCAGAGTTAAAGGTTAAGCCCTTGAAAGAGCTGACTTTCAATTCCCGTAATATTACAGCAACTATGACTTCTCGCCAGTTTCAGGTTATGCTGGATGTGTTAACAAATCTTCTCTTTGCACGGCTTCCCAAGCCTAGAAAAAGTAGTCTGTCTCTTCCTGCTGAAGATGATGAAGATGTGGAGGAGGAGGCAGATGAGGTTGTTCCTGATGGTGTTGAAGAGGTAGAGCTTGCAAAAGTGGACCTTGAGCAGAAAGAGCGGGAGCAGAAATTGGTTCTTGGTGACATAAGGAAATTGTCTCTTCGGTGTGATACCACTGGGGACCTATATCCAGAAAAGGAAGGGGACTTGTGGATGATAAATTGTACAAGATCCACTCTGGTGCAAGGACTTAAAAGAGAGCTTGTGAATTCCAAAAAGTCAAGGAAAGCATCATATGCATCATTAAGAATGGCTCTACATAAGGCTGCCCAGCTACGCCTGATGGAAAAAGAGAAGAACAAAAGTCCATCCTATGCTATGCGCATTTCTTTGCAAATTAACAAAGTGGTTTGGAGCATGATTGTTGATGGTAAATCTTTTGCCGAGGCTGAGATCAATGACATGATTTATGACTTTGACCGGGATTACAAAGATGTTGGTGTTGCTCAGTTTACAACAAAAAATTTTGTTGTCAGAAACTGCCTGCCAAATGCCAAATCAGATATGCTTTTATCAGCATGGAATCCTCCTACTGAATGGGAAAAGAAGGTTTTGCTTCGCGTGGATGCAAAGCAAGGAGCTCCTAAGGATGGAAATTCTTCTCTTGAACGTTTTCAGGTAGAGATCTATCCTCTCAAGATTCATTTAACTGAGGCAATGTACAGAATGATGTGGGGATATTTATTCCCAGAAGAAGAACAAGATTCACAAAGACGGCAGGAAGTTTGGAAGGTTTCAACAACTGCTGGTGCAAAACGTGTGAAGAAAGGCTCATTAGTCCCAGAGATTTCTGCATTAAGCGGTCAAACAAATAAAGAGTCTGAGGCTTCATCCAAAGCAAGTGCCTCTGCCCCTGCTACCAGTCAATCCTCCGTTCATGCAGATCCTGTGCAAGAATCAAAATTGCAAAACCTAAAGACAACAGTTGGCGGTAGTCCAACCAGAGAGTTAAGAAGAACGTCTTCCTTTGACAGATCATGGGAAGACACTGTGGCAGAATCAGTTGCTACAGAACTTGTCTTACAGAGCATTACTGGGCCACTTGGTTCTATTGAACCAGATGAGTCCTCAAAGAATAAATTGAAAGACCCCAAAGCTGTCAAATCTGGTAGGTCATCCCATGAAGAAAAGAAGGTAACAAAATCACAAGAAGAGAAAAGATCTAGGCCGCGAAAGATGATGGAGTTTCACAATATCAAGATAAGCCAGGTTGAGCTATGTGTTACTTACGAAGGATCTAGATTTGTTGTAAATGATCTTAAGTTGCTGATGGATACATTTCACCGTGTTGAGTTCACCGGCACTTGGCGGAGACTCTTTTCGCGGGTTAAGAAGCATATCATTTGGGGAGTCTTGAAGTCTGTGACGGGAATGCAGGGTAAGAAGTTCAAGGACAAGGCTAATAGTCAGAGGGAACCCAGTGGATCGGGTGTTCCTGATAGTGACCTTAATTTCAGTGACAATGAAGGTCAGCCAGAACAGCCTGACCAGAATCCTATACCGTTTCTCAAGCGTCCAAGTGACGGAGCAGGTGATGGATTTGTCACTTCCATCAGAGGCCTTTTTAATACTCAACGTCGCAAGGCCAAGGCATTCGTACTGCGAACCATGAGGGGTGAAGCAGAGAATGATTTCCAAGGAGATTGGAGTGAAAGTGATGCAGAGTTCTCTCCATTTGCTAGGCAGCTCACTATAACGAAAGCTAAGAGACTTATCCGGCGGCATACAAAAAAATTCCGTTCAAGAAAAGGTTCATCCTCGCAACAGAGAGATTCACTCGCATCCTCGCCAAGGGAGACCACTGCATTCGAGAGTGATTCTTCGAGTGGATCCTCGCCCTACGAAGATTTTAATGAGGGAAACATTTTGTCCTCCAGAGAGGTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0005575 GO:0005623 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0009267 GO:0009605 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0009991 GO:0010015 GO:0010053 GO:0010054 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0016036 GO:0016043 GO:0016049 GO:0021700 GO:0022414 GO:0022622 GO:0023051 GO:0023057 GO:0030154 GO:0030307 GO:0030427 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035618 GO:0035619 GO:0035838 GO:0040007 GO:0040008 GO:0042594 GO:0042995 GO:0044463 GO:0044464 GO:0044706 GO:0045927 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051286 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0070297 GO:0070298 GO:0071496 GO:0071695 GO:0071840 GO:0080147 GO:0090404 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0120038 GO:1902531 GO:1902532 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Apt1 Fmp27_GFWDK

Protein Analysis

2677

Amino Acids

298.57

Weight (kDa)

8.81

Isoelectric Point (pI)

46.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hobbit	PF10344	5 - 2597	0.0	Hobbit/FMP27/BTLP2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006211)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58250 AT1G58250 AT5G49680
fragaria_vesca	FvH4_5g34390
malus_domestica	MD08G1201600.v1.1 MD08G1201800.v1.1 MD08G1202400.v1.1 MD08G1208300.v1.1 MD15G1388100.v1.1
prunus_persica	Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1 Prupe.1G535700_v2.0.a1
pyrus_communis	pycom15g34840
rosa_chinensis	RchiOBHm_Chr7g0235041
rosa_laevigata	RLG00000001185
rosa_multiflora	Rmu_co8243721.1_g000001 Rmu_sc0002239.1_g000001 Rmu_sc0013687.1_g000001 Rmu_sc0015410.1_g000007
rosa_roxburghii	Rroxscaffold_3G00226360
rosa_rugosa	Rorug07G0287300
rosa_samantha	Rh7AG442100 Rh7BG414600 Rh7CG463100 Rh7DG431700
rosa_wichuraiana	Rw7G036740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1250, 3171
AarI	CACCTGC	1 cut(s)	7661
Acc16I	TGCGCA	1 cut(s)	6464
Acc36I	ACCTGC	4 cut(s)	3437, 3715, 4280, 7661
AccB1I	GGYRCC	2 cut(s)	3233, 4915
AccB7I	CCANNNNNTGG	2 cut(s)	1371, 5813
AccBSI	CCGCTC	1 cut(s)	6199
AccI	GTMKAC	2 cut(s)	3564, 4661
AccII	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
AclI	AACGTT	3 cut(s)	837, 1245, 6751
AcoI	YGGCCR	2 cut(s)	3970, 5623
AcuI	CTGAAG	7 cut(s)	931, 2351, 3243, 3699, 4191, 5642, 6123
AcvI	CACGTG	1 cut(s)	2654
AcyI	GRCGYC	2 cut(s)	1825, 4916
AdeI	CACNNNGTG	1 cut(s)	1964
AflII	CTTAAG	2 cut(s)	4009, 7400
AflIII	ACRYGT	5 cut(s)	1376, 2653, 4553, 5770, 6898
AhdI	GACNNNNNGTC	1 cut(s)	2020
AhlI	ACTAGT	2 cut(s)	2317, 4754
AjiI	CACGTC	1 cut(s)	967
AjnI	CCWGG	7 cut(s)	2392, 2878, 4701, 4934, 5238, 5806, 7347
AjuI	GAANNNNNNNTTGG	8 cut(s)	1664, 1696, 3564, 3596, 3672, 3704, 5559, 5591
AleI	CACNNNNGTG	2 cut(s)	5931, 6320
AloI	GAACNNNNNNTCC	2 cut(s)	7557, 7589
Alw21I	GWGCWC	1 cut(s)	6724
AlwNI	CAGNNNCTG	4 cut(s)	1394, 5813, 6621, 7028
Ama87I	CYCGRG	2 cut(s)	2004, 5678
ApeKI	GCWGC	7 cut(s)	277, 2822, 2950, 4544, 5612, 6407, 7825
ArsI	GACNNNNNNTTYG	4 cut(s)	956, 988, 6944, 6976
AseI	ATTAAT	1 cut(s)	1605
Asp700I	GAANNNNTTC	7 cut(s)	3249, 5772, 6739, 6753, 7102, 7772, 7891
AspLEI	GCGC	9 cut(s)	188, 1278, 2094, 2769, 2771, 4379, 4918, 5791, 6465
AsuC2I	CCSGG	4 cut(s)	2005, 2006, 5627, 6563
AvaI	CYCGRG	2 cut(s)	2004, 5678
AxyI	CCTNAGG	2 cut(s)	4725, 6726
BamHI	GGATCC	2 cut(s)	614, 7970
BanI	GGYRCC	2 cut(s)	3233, 4915
BanII	GRGCYC	2 cut(s)	5565, 6724
BarI	GAAGNNNNNNTAC	2 cut(s)	343, 375
BauI	CACGAG	3 cut(s)	164, 4182, 5228
BbrPI	CACGTG	1 cut(s)	2654
BbsI	GAAGAC	6 cut(s)	358, 1668, 1782, 4183, 7095, 7131
Bbv12I	GWGCWC	1 cut(s)	6724
BbvCI	CCTCAGC	1 cut(s)	2511
BbvI	GCAGC	7 cut(s)	264, 2809, 2937, 4531, 5599, 6394, 7837
BceAI	ACGGC	4 cut(s)	2240, 3957, 6077, 6872
BciT130I	CCWGG	7 cut(s)	2394, 2880, 4703, 4936, 5240, 5808, 7349
BciVI	GTATCC	4 cut(s)	1031, 4075, 5051, 7408
BclI	TGATCA	2 cut(s)	732, 5038
BcnI	CCSGG	4 cut(s)	2005, 2006, 5627, 6563
BcuI	ACTAGT	2 cut(s)	2317, 4754
BfmI	CTRYAG	7 cut(s)	539, 2333, 2481, 2694, 3238, 5487, 7149
BfoI	RGCGCY	3 cut(s)	189, 4380, 4919
BfrI	CTTAAG	2 cut(s)	4009, 7400
BfuAI	ACCTGC	4 cut(s)	3437, 3715, 4280, 7661
BfuI	GTATCC	4 cut(s)	1031, 4075, 5051, 7408
BglII	AGATCT	4 cut(s)	2141, 4487, 6765, 7301
BmcAI	AGTACT	2 cut(s)	3274, 4753
BmeRI	GACNNNNNGTC	1 cut(s)	2020
BmeT110I	CYCGRG	2 cut(s)	2004, 5678
BmgBI	CACGTC	1 cut(s)	967
BmrI	ACTGGG	5 cut(s)	1639, 5706, 6267, 7185, 7554
BmuI	ACTGGG	5 cut(s)	1639, 5706, 6267, 7185, 7554
BoxI	GACNNNNGTC	1 cut(s)	5082
BpiI	GAAGAC	6 cut(s)	358, 1668, 1782, 4183, 7095, 7131
BplI	GAGNNNNNCTC	2 cut(s)	7902, 7934
BpmI	CTGGAG	5 cut(s)	630, 4071, 5340, 5925, 7999
Bpu10I	CCTNAGC	4 cut(s)	273, 1938, 2511, 4593
BpuEI	CTTGAG	7 cut(s)	3072, 3543, 4994, 5900, 6206, 6761, 7634
BpuMI	CCSGG	4 cut(s)	2005, 2006, 5627, 6563
BsaAI	YACGTR	2 cut(s)	2654, 3470
BsaBI	GATNNNNATC	2 cut(s)	4584, 5802
BsaHI	GRCGYC	2 cut(s)	1825, 4916
BsaI	GGTCTC	2 cut(s)	5327, 7931
BsaWI	WCCGGW	5 cut(s)	132, 2176, 3382, 4067, 5913
BsaXI	ACNNNNNCTCC	4 cut(s)	5002, 5032, 7780, 7810
Bse118I	RCCGGY	1 cut(s)	7439
Bse21I	CCTNAGG	2 cut(s)	4725, 6726
Bse3DI	GCAATG	6 cut(s)	747, 1764, 2427, 2541, 4073, 6804
Bse8I	GATNNNNATC	2 cut(s)	4584, 5802
BseBI	CCWGG	7 cut(s)	2394, 2880, 4703, 4936, 5240, 5808, 7349
BseJI	GATNNNNATC	2 cut(s)	4584, 5802
BseMI	GCAATG	6 cut(s)	747, 1764, 2427, 2541, 4073, 6804
BsePI	GCGCGC	1 cut(s)	2767
BseXI	GCAGC	7 cut(s)	264, 2809, 2937, 4531, 5599, 6394, 7837
BseYI	CCCAGC	2 cut(s)	4930, 6411
BsgI	GTGCAG	1 cut(s)	4530
Bsh1236I	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
BshNI	GGYRCC	2 cut(s)	3233, 4915
BsiHKAI	GWGCWC	1 cut(s)	6724
BsiHKCI	CYCGRG	2 cut(s)	2004, 5678
BsiWI	CGTACG	1 cut(s)	359
BslFI	GGGAC	7 cut(s)	1864, 4611, 4803, 5545, 6275, 6299, 6905
BsmFI	GGGAC	7 cut(s)	1864, 4611, 4803, 5545, 6275, 6299, 6905
BsmI	GAATGC	6 cut(s)	1559, 2572, 4676, 7518, 7733, 7946
Bso31I	GGTCTC	2 cut(s)	5327, 7931
BsoBI	CYCGRG	2 cut(s)	2004, 5678
Bsp1286I	GDGCHC	2 cut(s)	5565, 6724
Bsp1407I	TGTACA	6 cut(s)	1501, 2305, 2350, 3127, 6305, 6801
Bsp19I	CCATGG	3 cut(s)	1268, 3818, 5299
BspFNI	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
BspHI	TCATGA	2 cut(s)	709, 4381
BspMI	ACCTGC	4 cut(s)	3437, 3715, 4280, 7661
BspQI	GCTCTTC	2 cut(s)	1798, 2937
BspT107I	GGYRCC	2 cut(s)	3233, 4915
BspTI	CTTAAG	2 cut(s)	4009, 7400
BspTNI	GGTCTC	2 cut(s)	5327, 7931
BsrBI	CCGCTC	1 cut(s)	6199
BsrDI	GCAATG	6 cut(s)	747, 1764, 2427, 2541, 4073, 6804
BsrFI	RCCGGY	1 cut(s)	7439
BsrGI	TGTACA	6 cut(s)	1501, 2305, 2350, 3127, 6305, 6801
BssAI	RCCGGY	1 cut(s)	7439
BssHII	GCGCGC	1 cut(s)	2767
BssNAI	GTATAC	2 cut(s)	3565, 4662
BssNI	GRCGYC	2 cut(s)	1825, 4916
BssSI	CACGAG	3 cut(s)	164, 4182, 5228
BssT1I	CCWWGG	7 cut(s)	237, 1268, 3818, 5299, 7728, 7777, 7931
Bst1107I	GTATAC	2 cut(s)	3565, 4662
Bst2BI	CACGAG	3 cut(s)	164, 4182, 5228
Bst2UI	CCWGG	7 cut(s)	2394, 2880, 4703, 4936, 5240, 5808, 7349
BstACI	GRCGYC	2 cut(s)	1825, 4916
BstAFI	CTTAAG	2 cut(s)	4009, 7400
BstAPI	GCANNNNNTGC	2 cut(s)	4166, 6377
BstAUI	TGTACA	6 cut(s)	1501, 2305, 2350, 3127, 6305, 6801
BstBAI	YACGTR	2 cut(s)	2654, 3470
BstDSI	CCRYGG	4 cut(s)	575, 1268, 3818, 5299
BstENI	CCTNNNNNAGG	4 cut(s)	779, 3328, 4170, 7930
BstFNI	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
BstH2I	RGCGCY	3 cut(s)	189, 4380, 4919
BstHHI	GCGC	9 cut(s)	188, 1278, 2094, 2769, 2771, 4379, 4918, 5791, 6465
BstNI	CCWGG	7 cut(s)	2394, 2880, 4703, 4936, 5240, 5808, 7349
BstNSI	RCATGY	6 cut(s)	1380, 3204, 3607, 4557, 5330, 5774
BstPAI	GACNNNNGTC	1 cut(s)	5082
BstSFI	CTRYAG	7 cut(s)	539, 2333, 2481, 2694, 3238, 5487, 7149
BstUI	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
BstV1I	GCAGC	7 cut(s)	264, 2809, 2937, 4531, 5599, 6394, 7837
BstV2I	GAAGAC	6 cut(s)	358, 1668, 1782, 4183, 7095, 7131
BstZ17I	GTATAC	2 cut(s)	3565, 4662
Bsu36I	CCTNAGG	2 cut(s)	4725, 6726
BsuI	GTATCC	4 cut(s)	1031, 4075, 5051, 7408
BtgI	CCRYGG	4 cut(s)	575, 1268, 3818, 5299
BtgZI	GCGATG	1 cut(s)	1443
BtrI	CACGTC	1 cut(s)	967
BtsI	GCAGTG	2 cut(s)	5828, 7941
BveI	ACCTGC	4 cut(s)	3437, 3715, 4280, 7661
CaiI	CAGNNNCTG	4 cut(s)	1394, 5813, 6621, 7028
CciI	TCATGA	2 cut(s)	709, 4381
CfoI	GCGC	9 cut(s)	188, 1278, 2094, 2769, 2771, 4379, 4918, 5791, 6465
Cfr10I	RCCGGY	1 cut(s)	7439
Cfr9I	CCCGGG	1 cut(s)	2004
CpoI	CGGWCCG	1 cut(s)	1667
CseI	GACGC	4 cut(s)	1814, 2690, 3382, 7643
CspCI	CAANNNNNGTGG	2 cut(s)	2104, 2139
CspI	CGGWCCG	1 cut(s)	1667
DinI	GGCGCC	1 cut(s)	4917
DraI	TTTAAA	4 cut(s)	2904, 4047, 4560, 4737
DraIII	CACNNNGTG	1 cut(s)	1964
DriI	GACNNNNNGTC	1 cut(s)	2020
EaeI	YGGCCR	2 cut(s)	3970, 5623
Eam1105I	GACNNNNNGTC	1 cut(s)	2020
EciI	GGCGGA	2 cut(s)	156, 7465
Ecl136II	GAGCTC	1 cut(s)	6722
Eco130I	CCWWGG	7 cut(s)	237, 1268, 3818, 5299, 7728, 7777, 7931
Eco147I	AGGCCT	3 cut(s)	316, 2727, 7701
Eco24I	GRGCYC	2 cut(s)	5565, 6724
Eco31I	GGTCTC	2 cut(s)	5327, 7931
Eco32I	GATATC	1 cut(s)	546
Eco53kI	GAGCTC	1 cut(s)	6722
Eco57I	CTGAAG	7 cut(s)	931, 2351, 3243, 3699, 4191, 5642, 6123
Eco72I	CACGTG	1 cut(s)	2654
Eco81I	CCTNAGG	2 cut(s)	4725, 6726
Eco88I	CYCGRG	2 cut(s)	2004, 5678
EcoICRI	GAGCTC	1 cut(s)	6722
EcoNI	CCTNNNNNAGG	4 cut(s)	779, 3328, 4170, 7930
EcoO109I	RGGNCCY	4 cut(s)	846, 4248, 4817, 6262
EcoRI	GAATTC	2 cut(s)	2209, 6349
EcoRII	CCWGG	7 cut(s)	2392, 2878, 4701, 4934, 5238, 5806, 7347
EcoRV	GATATC	1 cut(s)	546
EcoT14I	CCWWGG	7 cut(s)	237, 1268, 3818, 5299, 7728, 7777, 7931
EcoT22I	ATGCAT	2 cut(s)	3481, 6382
EcoT38I	GRGCYC	2 cut(s)	5565, 6724
EgeI	GGCGCC	1 cut(s)	4917
EheI	GGCGCC	1 cut(s)	4917
ErhI	CCWWGG	7 cut(s)	237, 1268, 3818, 5299, 7728, 7777, 7931
FaqI	GGGAC	7 cut(s)	1864, 4611, 4803, 5545, 6275, 6299, 6905
FauI	CCCGC	2 cut(s)	6192, 7458
FauNDI	CATATG	5 cut(s)	1506, 4566, 4719, 4984, 6376
FbaI	TGATCA	2 cut(s)	732, 5038
FblI	GTMKAC	2 cut(s)	3564, 4661
FriOI	GRGCYC	2 cut(s)	5565, 6724
FspAI	RTGCGCAY	1 cut(s)	6464
FspI	TGCGCA	1 cut(s)	6464
GlaI	GCGC	9 cut(s)	187, 1277, 2093, 2768, 2770, 4378, 4917, 5790, 6464
GsaI	CCCAGC	2 cut(s)	4934, 6415
GsuI	CTGGAG	5 cut(s)	630, 4071, 5340, 5925, 7999
HaeII	RGCGCY	3 cut(s)	189, 4380, 4919
HgaI	GACGC	4 cut(s)	1814, 2690, 3382, 7643
HhaI	GCGC	9 cut(s)	188, 1278, 2094, 2769, 2771, 4379, 4918, 5791, 6465
Hin1I	GRCGYC	2 cut(s)	1825, 4916
Hin6I	GCGC	9 cut(s)	186, 1276, 2092, 2767, 2769, 4377, 4916, 5789, 6463
HinP1I	GCGC	9 cut(s)	186, 1276, 2092, 2767, 2769, 4377, 4916, 5789, 6463
HincII	GTYRAC	2 cut(s)	2925, 6042
HindII	GTYRAC	2 cut(s)	2925, 6042
HindIII	AAGCTT	5 cut(s)	1223, 2363, 2810, 3917, 5111
HpaI	GTTAAC	2 cut(s)	2925, 6042
Hpy99I	CGWCG	3 cut(s)	2684, 5324, 7722
Hsp92I	GRCGYC	2 cut(s)	1825, 4916
HspAI	GCGC	9 cut(s)	186, 1276, 2092, 2767, 2769, 4377, 4916, 5789, 6463
KasI	GGCGCC	1 cut(s)	4915
Ksp22I	TGATCA	2 cut(s)	732, 5038
KspAI	GTTAAC	2 cut(s)	2925, 6042
LguI	GCTCTTC	2 cut(s)	1798, 2937
LmnI	GCTCC	9 cut(s)	66, 116, 4090, 4536, 6202, 6495, 6719, 6727, 7667
Lsp1109I	GCAGC	7 cut(s)	264, 2809, 2937, 4531, 5599, 6394, 7837
MbiI	CCGCTC	1 cut(s)	6199
MfeI	CAATTG	2 cut(s)	1088, 5080
MhlI	GDGCHC	2 cut(s)	5565, 6724
Mly113I	GGCGCC	1 cut(s)	4916
MlyI	GAGTC	7 cut(s)	1748, 1846, 5527, 6968, 7214, 7449, 7500
MmeI	TCCRAC	4 cut(s)	1591, 2703, 5745, 7103
Mph1103I	ATGCAT	2 cut(s)	3481, 6382
MroXI	GAANNNNTTC	7 cut(s)	3249, 5772, 6739, 6753, 7102, 7772, 7891
MslI	CAYNNNNRTG	9 cut(s)	473, 2379, 4335, 4552, 4866, 5046, 5250, 5931, 6320
MspA1I	CMGCKG	3 cut(s)	1858, 2950, 2980
MspCI	CTTAAG	2 cut(s)	4009, 7400
MunI	CAATTG	2 cut(s)	1088, 5080
Mva1269I	GAATGC	6 cut(s)	1559, 2572, 4676, 7518, 7733, 7946
MvaI	CCWGG	7 cut(s)	2394, 2880, 4703, 4936, 5240, 5808, 7349
MvnI	CGCG	6 cut(s)	108, 2094, 2769, 6702, 7312, 7465
NarI	GGCGCC	1 cut(s)	4916
NciI	CCSGG	4 cut(s)	2005, 2006, 5627, 6563
NcoI	CCATGG	3 cut(s)	1268, 3818, 5299
NdeI	CATATG	5 cut(s)	1506, 4566, 4719, 4984, 6376
NmeAIII	GCCGAG	2 cut(s)	1844, 6550
NsbI	TGCGCA	1 cut(s)	6464
NsiI	ATGCAT	2 cut(s)	3481, 6382
NspI	RCATGY	6 cut(s)	1380, 3204, 3607, 4557, 5330, 5774
OliI	CACNNNNGTG	2 cut(s)	5931, 6320
PaeI	GCATGC	2 cut(s)	3204, 5330
PaeR7I	CTCGAG	1 cut(s)	5678
PagI	TCATGA	2 cut(s)	709, 4381
PaqCI	CACCTGC	1 cut(s)	7661
PauI	GCGCGC	1 cut(s)	2767
PceI	AGGCCT	3 cut(s)	316, 2727, 7701
PciI	ACATGT	3 cut(s)	1376, 4553, 5770
PciSI	GCTCTTC	2 cut(s)	1798, 2937
PcsI	WCGNNNNNNNCGW	2 cut(s)	355, 4459
PctI	GAATGC	6 cut(s)	1559, 2572, 4676, 7518, 7733, 7946
PdmI	GAANNNNTTC	7 cut(s)	3249, 5772, 6739, 6753, 7102, 7772, 7891
Pfl23II	CGTACG	1 cut(s)	359
PflMI	CCANNNNNTGG	2 cut(s)	1371, 5813
PleI	GAGTC	7 cut(s)	1747, 1846, 5527, 6967, 7213, 7449, 7499
PluTI	GGCGCC	1 cut(s)	4919
PmaCI	CACGTG	1 cut(s)	2654
PmlI	CACGTG	1 cut(s)	2654
PpsI	GAGTC	7 cut(s)	1747, 1846, 5527, 6967, 7213, 7449, 7499
Ppu21I	YACGTR	2 cut(s)	2654, 3470
PpuMI	RGGWCCY	3 cut(s)	846, 4817, 6262
PscI	ACATGT	3 cut(s)	1376, 4553, 5770
PshAI	GACNNNNGTC	1 cut(s)	5082
PshBI	ATTAAT	1 cut(s)	1605
PsiI	TTATAA	2 cut(s)	1250, 3171
Psp124BI	GAGCTC	1 cut(s)	6724
Psp1406I	AACGTT	3 cut(s)	837, 1245, 6751
Psp5II	RGGWCCY	3 cut(s)	846, 4817, 6262
Psp6I	CCWGG	7 cut(s)	2392, 2878, 4701, 4934, 5238, 5806, 7347
PspCI	CACGTG	1 cut(s)	2654
PspFI	CCCAGC	2 cut(s)	4930, 6411
PspGI	CCWGG	7 cut(s)	2392, 2878, 4701, 4934, 5238, 5806, 7347
PspLI	CGTACG	1 cut(s)	359
PspPPI	RGGWCCY	3 cut(s)	846, 4817, 6262
PspXI	VCTCGAGB	1 cut(s)	5678
PsrI	GAACNNNNNNTAC	6 cut(s)	2289, 2316, 2321, 2348, 7146, 7178
PstNI	CAGNNNCTG	4 cut(s)	1394, 5813, 6621, 7028
PteI	GCGCGC	1 cut(s)	2767
PvuII	CAGCTG	2 cut(s)	1858, 2980
RseI	CAYNNNNRTG	9 cut(s)	473, 2379, 4335, 4552, 4866, 5046, 5250, 5931, 6320
Rsr2I	CGGWCCG	1 cut(s)	1667
RsrII	CGGWCCG	1 cut(s)	1667
SacI	GAGCTC	1 cut(s)	6724
SapI	GCTCTTC	2 cut(s)	1798, 2937
ScaI	AGTACT	2 cut(s)	3274, 4753
SchI	GAGTC	7 cut(s)	1748, 1846, 5527, 6968, 7214, 7449, 7500
SduI	GDGCHC	2 cut(s)	5565, 6724
SfcI	CTRYAG	7 cut(s)	539, 2333, 2481, 2694, 3238, 5487, 7149
SfoI	GGCGCC	1 cut(s)	4917
Sfr274I	CTCGAG	1 cut(s)	5678
SlaI	CTCGAG	1 cut(s)	5678
SmaI	CCCGGG	1 cut(s)	2006
SmiMI	CAYNNNNRTG	9 cut(s)	473, 2379, 4335, 4552, 4866, 5046, 5250, 5931, 6320
SpeI	ACTAGT	2 cut(s)	2317, 4754
SphI	GCATGC	2 cut(s)	3204, 5330
SseBI	AGGCCT	3 cut(s)	316, 2727, 7701
SspDI	GGCGCC	1 cut(s)	4915
SspI	AATATT	4 cut(s)	409, 3433, 5710, 5992
SstI	GAGCTC	1 cut(s)	6724
StuI	AGGCCT	3 cut(s)	316, 2727, 7701
StyI	CCWWGG	7 cut(s)	237, 1268, 3818, 5299, 7728, 7777, 7931
TaqI	TCGA	9 cut(s)	320, 349, 1002, 1969, 3415, 5322, 5679, 7950, 7964
TauI	GCSGC	6 cut(s)	8, 108, 2950, 4573, 7312, 7864
TseI	GCWGC	7 cut(s)	277, 2822, 2950, 4544, 5612, 6407, 7825
TspGWI	ACGGA	7 cut(s)	1818, 4339, 4823, 5308, 7003, 7680, 7868
TspMI	CCCGGG	1 cut(s)	2004
Van91I	CCANNNNNTGG	2 cut(s)	1371, 5813
Vha464I	CTTAAG	2 cut(s)	4009, 7400
VspI	ATTAAT	1 cut(s)	1605
XagI	CCTNNNNNAGG	4 cut(s)	779, 3328, 4170, 7930
XbaI	TCTAGA	1 cut(s)	7379
XceI	RCATGY	6 cut(s)	1380, 3204, 3607, 4557, 5330, 5774
XcmI	CCANNNNNNNNNTGG	4 cut(s)	439, 1057, 1504, 5246
XhoI	CTCGAG	1 cut(s)	5678
XmaI	CCCGGG	1 cut(s)	2004
XmiI	GTMKAC	2 cut(s)	3564, 4661
XmnI	GAANNNNTTC	7 cut(s)	3249, 5772, 6739, 6753, 7102, 7772, 7891
ZrmI	AGTACT	2 cut(s)	3274, 4753
Zsp2I	ATGCAT	2 cut(s)	3481, 6382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.