AT5G50100

Protein of unknown function, DUF393

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010102
Physical Location & Seq
Forward (+)
20371798 .. 20373441
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50100.1

Sequence Viewer

Length: 645 bp
ATGGCAACGAGAGGAGCTGTTGCTGCTGCTGCATCCACAATATGGAAGCACCGAAGAAACCCTTCTCTTCGATCATTATCTCGCCATTTCAATCCCAATTTCAATCATAGAATCATCCCAACAGGGTTTAAATATCAAGTTAGGGCGATACAAGGAACAAGTACTGATCCTGTCATTACACCATTAAAGAACAGAGAGGAACCAAAGCCACAAAACTGGAAAATCAAAATGCTTTATGATGGAGATTGTCCTCTCTGTATGCGTGAGGTTAATATGCTTATGGAAAGGAATGAAAAACATGGAACTATAAAGTTTGTCGACATAAGTTCAAATGATTATTCCCCGGAAGATAATCAAGGGCTTGATTACAAAACAGTGATGGGGCAGATTCATGCCATTCAGTCTGATGGTAACGTTGTTAAGGGTGTGGAGGCATTTAGGAGATTGTATGAAGAAGTTGGGCTTGGATGGGTTTACACTATCACCAAATTTGAACCAATAGGGAAGTTAGCAGATGTTGTGTATGATGTATGGGCTAAATACCGACTTCAAGTCACAGGGAGGCCATCTATAGAAGCTATTCTTGAAGCAAGAAAGAAAGATAAGGTAGAGACATGTGGTGAAAGCAAGAACTGCAAGATATGA

Protein Analysis

214

Amino Acids

24.43

Weight (kDa)

9.3

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 78 - 188 1.7e-27 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 42
AccI GTMKAC 1 cut(s) 318
AclI AACGTT 1 cut(s) 414
AclWI GGATC 1 cut(s) 161
AcsI RAATTY 1 cut(s) 488
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 6 cut(s) 91, 103, 330, 494, 551, 587
AhdI GACNNNNNGTC 1 cut(s) 551
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AluBI AGCT 2 cut(s) 17, 578
AluI AGCT 2 cut(s) 17, 578
Alw26I GTCTC 1 cut(s) 605
AlwI GGATC 1 cut(s) 161
AoxI GGCC 1 cut(s) 563
ApeKI GCWGC 3 cut(s) 23, 26, 29
ApoI RAATTY 1 cut(s) 488
Asp700I GAANNNNTTC 2 cut(s) 61, 579
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 475, 632
BbvI GCAGC 3 cut(s) 10, 13, 16
BccI CCATC 5 cut(s) 233, 373, 401, 462, 574
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 570
BisI GCNGC 3 cut(s) 24, 27, 30
BlsI GCNGC 3 cut(s) 25, 28, 31
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 344
BmeRI GACNNNNNGTC 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 344
BsaBI GATNNNNATC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse1I ACTGG 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 3 cut(s) 32, 114, 473
BseJI GATNNNNATC 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 3 cut(s) 10, 13, 16
BshFI GGCC 1 cut(s) 565
BsiSI CCGG 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 605
BsnI GGCC 1 cut(s) 565
Bsp143I GATC 2 cut(s) 71, 166
BspANI GGCC 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 2 cut(s) 71, 166
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 633
BstF5I GGATG 3 cut(s) 32, 114, 473
BstKTI GATC 2 cut(s) 74, 169
BstMAI GTCTC 1 cut(s) 605
BstMBI GATC 2 cut(s) 71, 166
BstMWI GCNNNNNNNGC 3 cut(s) 23, 29, 633
BstNSI RCATGY 1 cut(s) 618
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 3 cut(s) 10, 13, 16
BstXI CCANNNNNNTGG 1 cut(s) 216
BsuRI GGCC 1 cut(s) 565
BtsCI GGATG 3 cut(s) 32, 114, 473
BtsIMutI CAGTG 1 cut(s) 381
Csp6I GTAC 1 cut(s) 162
CviAII CATG 3 cut(s) 299, 392, 615
CviJI RGCY 7 cut(s) 17, 208, 361, 463, 536, 565, 578
CviKI_1 RGCY 7 cut(s) 17, 208, 361, 463, 536, 565, 578
CviQI GTAC 1 cut(s) 162
DpnI GATC 2 cut(s) 73, 168
DpnII GATC 2 cut(s) 71, 166
DraI TTTAAA 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 551
Eam1104I CTCTTC 1 cut(s) 72
Eam1105I GACNNNNNGTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 72
FaeI CATG 3 cut(s) 302, 395, 618
FalI AAGNNNNNCTT 6 cut(s) 46, 78, 447, 479, 567, 599
FatI CATG 3 cut(s) 298, 391, 614
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 3 cut(s) 24, 27, 30
FokI GGATG 3 cut(s) 19, 101, 480
Fsp4HI GCNGC 3 cut(s) 24, 27, 30
GluI GCNGC 3 cut(s) 24, 27, 30
HaeIII GGCC 1 cut(s) 565
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 302, 395, 618
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 2 cut(s) 111, 388
HpaII CCGG 1 cut(s) 344
HphI GGTGA 2 cut(s) 475, 632
Hpy166II GTNNAC 2 cut(s) 319, 475
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 1 cut(s) 584
Hpy8I GTNNAC 2 cut(s) 319, 475
HpyAV CCTTC 1 cut(s) 72
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 2 cut(s) 32, 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 29, 633
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 3 cut(s) 302, 395, 618
Kzo9I GATC 2 cut(s) 71, 166
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 5 cut(s) 108, 183, 202, 357, 543
Lsp1109I GCAGC 3 cut(s) 10, 13, 16
LweI GCATC 1 cut(s) 41
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 410, 553
MalI GATC 2 cut(s) 73, 168
MboI GATC 2 cut(s) 71, 166
MboII GAAGA 4 cut(s) 59, 66, 359, 464
MluCI AATT 2 cut(s) 97, 488
MnlI CCTC 6 cut(s) 5, 190, 259, 261, 424, 555
MroXI GAANNNNTTC 2 cut(s) 61, 579
MseI TTAA 4 cut(s) 129, 185, 270, 420
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 23, 29, 633
NciI CCSGG 1 cut(s) 344
NdeII GATC 2 cut(s) 71, 166
NlaIII CATG 3 cut(s) 302, 395, 618
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 553
NspI RCATGY 1 cut(s) 618
PciI ACATGT 1 cut(s) 614
PdmI GAANNNNTTC 2 cut(s) 61, 579
PfeI GAWTC 2 cut(s) 111, 388
PflMI CCANNNNNTGG 1 cut(s) 42
PkrI GCNGC 3 cut(s) 25, 28, 31
PscI ACATGT 1 cut(s) 614
Psp1406I AACGTT 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 201
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 4 cut(s) 129, 185, 270, 420
SatI GCNGC 3 cut(s) 24, 27, 30
Sau3AI GATC 2 cut(s) 71, 166
ScaI AGTACT 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 344
SetI ASST 5 cut(s) 19, 270, 417, 580, 609
SfaNI GCATC 1 cut(s) 41
SfcI CTRYAG 1 cut(s) 570
Sse9I AATT 2 cut(s) 97, 488
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 417
TaqI TCGA 2 cut(s) 70, 318
TasI AATT 2 cut(s) 97, 488
TatI WGTACW 1 cut(s) 161
TfiI GAWTC 2 cut(s) 111, 388
Tru1I TTAA 4 cut(s) 129, 185, 270, 420
Tru9I TTAA 4 cut(s) 129, 185, 270, 420
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 3 cut(s) 23, 26, 29
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 3 cut(s) 306, 380, 465
TspRI CASTG 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 42
XapI RAATTY 1 cut(s) 488
XceI RCATGY 1 cut(s) 618
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 2 cut(s) 61, 579
ZrmI AGTACT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.