MD06G1180000.v1.1

protein At5g50100, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010102
Physical Location & Seq
Forward (+)
31949545 .. 31952065
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1180000.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCAATGAGGGCAGCTGCTTCAGCATGTAAAATTGGAGGAAGGCAAAGAAACCCTTCTCTCTCTTCAGTTCCTCTCTCTCTCCAACAACTCAAGCTCCATCAAATTCAACCCCAAAGATTCATATCAGCCCCTCCTATTAACCAACCTGGGTTTAAGTCTTCCGTAAGGGCTATAAGTGGGGCAGCTGCAGATCCTTTGACTCCAAAGAAAGAAAATGATCGAGAACAAACACCTCAGAATTGGAAGATTAAAATGCTCTATGACGGGGATTGCCCCTTGTGCATGCGAGAGGTGGACATGCTGAGAGAGAGAAATAAGCAATATGGTACCATCAAGTTTGTTGACATAAGTTCAGATGACTACTCGCCAGAGAAGAATCAAGGGCTGGACTACAAGACTGTTATGGGAAACATTCATGCCATCCTCTCTGATGGAACTGTGGTCACTGATGTCGAAGCATTCAGAAAGCTCTACGAGCAAGTGGGGCTCGGATGGGTATATGCCATAACCAAATACGAACCTATTGCAACAATAGCTGGTGCTGTATATGGCGTTTGGGCTAAATATCGTCTTCAAATCACAGGCCGGCCACCCCTAGAAGAGGTTTTGGAGTTGCGAAAGAAGAACAAGGGAGAAGTATGTAAAGATGCAAAAGATTGTAAACTTCCAGAGTAA

Protein Analysis

226

Amino Acids

25.2

Weight (kDa)

9.1

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 87 - 197 1.8e-25 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 1 cut(s) 329
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 1 cut(s) 105
AcuI CTGAAG 2 cut(s) 6, 51
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 1 cut(s) 604
AgsI TTSAA 2 cut(s) 110, 578
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 5 cut(s) 17, 97, 188, 472, 539
AluI AGCT 5 cut(s) 17, 97, 188, 472, 539
AlwI GGATC 1 cut(s) 188
AoxI GGCC 2 cut(s) 586, 590
ApeKI GCWGC 4 cut(s) 14, 17, 185, 188
ApoI RAATTY 1 cut(s) 105
Asp700I GAANNNNTTC 1 cut(s) 55
Asp718I GGTACC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 153, 566
BbvI GCAGC 4 cut(s) 4, 26, 175, 197
BccI CCATC 5 cut(s) 108, 341, 428, 431, 489
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 4 cut(s) 15, 18, 186, 189
BlsI GCNGC 4 cut(s) 16, 19, 187, 190
Bme1390I CCNGG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 640
BpiI GAAGAC 2 cut(s) 153, 566
BpuEI CTTGAG 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 604
Bse118I RCCGGY 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 2 cut(s) 423, 500
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 604
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 251, 296
BseXI GCAGC 4 cut(s) 4, 26, 175, 197
BshFI GGCC 2 cut(s) 588, 592
BshNI GGYRCC 1 cut(s) 329
BsiSI CCGG 1 cut(s) 589
BslI CCNNNNNNNGG 1 cut(s) 604
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 588, 592
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 193, 220
BspANI GGCC 2 cut(s) 588, 592
BspCNI CTCAG 2 cut(s) 250, 297
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 193
BspPI GGATC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 2 cut(s) 193, 220
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 403, 442
Bst6I CTCTTC 2 cut(s) 70, 597
BstC8I GCNNGC 2 cut(s) 287, 590
BstDEI CTNAG 2 cut(s) 237, 305
BstENI CCTNNNNNAGG 1 cut(s) 602
BstF5I GGATG 2 cut(s) 423, 500
BstKTI GATC 2 cut(s) 196, 223
BstMBI GATC 2 cut(s) 193, 220
BstMWI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 478, 487, 536
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 3 cut(s) 30, 289, 304
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 4, 26, 175, 197
BstV2I GAAGAC 2 cut(s) 153, 566
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 2 cut(s) 588, 592
BtsCI GGATG 2 cut(s) 423, 500
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 2 cut(s) 287, 590
Cfr10I RCCGGY 1 cut(s) 588
Csp6I GTAC 1 cut(s) 330
CviAII CATG 4 cut(s) 27, 286, 301, 419
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 237, 305
DpnI GATC 2 cut(s) 195, 222
DpnII GATC 2 cut(s) 193, 220
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 2 cut(s) 70, 597
EarI CTCTTC 2 cut(s) 70, 597
Eco24I GRGCYC 1 cut(s) 492
Eco57I CTGAAG 2 cut(s) 6, 51
EcoNI CCTNNNNNAGG 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 492
FaeI CATG 4 cut(s) 30, 289, 304, 422
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FatI CATG 4 cut(s) 26, 285, 300, 418
Fnu4HI GCNGC 4 cut(s) 15, 18, 186, 189
FokI GGATG 2 cut(s) 410, 507
FriOI GRGCYC 1 cut(s) 492
FseI GGCCGGCC 1 cut(s) 592
Fsp4HI GCNGC 4 cut(s) 15, 18, 186, 189
FspBI CTAG 1 cut(s) 599
GluI GCNGC 4 cut(s) 15, 18, 186, 189
HaeIII GGCC 2 cut(s) 588, 592
HapII CCGG 1 cut(s) 589
Hin1II CATG 4 cut(s) 30, 289, 304, 422
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 120, 202, 379
HpaII CCGG 1 cut(s) 589
Hpy166II GTNNAC 3 cut(s) 298, 346, 665
Hpy188I TCNGA 5 cut(s) 240, 358, 433, 467, 494
Hpy188III TCNNGA 2 cut(s) 224, 671
Hpy8I GTNNAC 3 cut(s) 298, 346, 665
HpyAV CCTTC 2 cut(s) 36, 66
HpyCH4III ACNGT 2 cut(s) 403, 442
HpyCH4V TGCA 4 cut(s) 191, 285, 530, 653
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 478, 487, 536
HpyF3I CTNAG 2 cut(s) 237, 305
Hsp92II CATG 4 cut(s) 30, 289, 304, 422
KpnI GGTACC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 588
KroNI GCCGGC 1 cut(s) 590
Kzo9I GATC 2 cut(s) 193, 220
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 7 cut(s) 135, 162, 374, 384, 525, 570, 602
Lsp1109I GCAGC 4 cut(s) 4, 26, 175, 197
LweI GCATC 1 cut(s) 640
MaeI CTAG 1 cut(s) 599
MaeIII GTNAC 1 cut(s) 445
MalI GATC 2 cut(s) 195, 222
MboI GATC 2 cut(s) 193, 220
MboII GAAGA 7 cut(s) 57, 153, 259, 388, 566, 614, 637
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 3 cut(s) 33, 105, 241
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 3, 32, 84, 144, 246, 286, 437, 598
MroNI GCCGGC 1 cut(s) 588
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 3 cut(s) 141, 156, 252
MspA1I CMGCKG 2 cut(s) 17, 188
MspI CCGG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 478, 487, 536
NaeI GCCGGC 1 cut(s) 590
NdeII GATC 2 cut(s) 193, 220
NgoMIV GCCGGC 1 cut(s) 588
NlaIII CATG 4 cut(s) 30, 289, 304, 422
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 445
NspI RCATGY 3 cut(s) 30, 289, 304
PaeI GCATGC 1 cut(s) 289
PctI GAATGC 1 cut(s) 461
PdiI GCCGGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 120, 379
PkrI GCNGC 4 cut(s) 16, 19, 187, 190
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 17, 188
RigI GGCCGGCC 1 cut(s) 592
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 141, 156, 252
SatI GCNGC 4 cut(s) 15, 18, 186, 189
Sau3AI GATC 2 cut(s) 193, 220
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 492
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SphI GCATGC 1 cut(s) 289
Sse9I AATT 3 cut(s) 33, 105, 241
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 403, 442
TaqI TCGA 2 cut(s) 223, 456
TasI AATT 3 cut(s) 33, 105, 241
TfiI GAWTC 2 cut(s) 120, 379
Tru1I TTAA 3 cut(s) 141, 156, 252
Tru9I TTAA 3 cut(s) 141, 156, 252
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 4 cut(s) 14, 17, 185, 188
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 2 cut(s) 112, 407
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 602
XapI RAATTY 1 cut(s) 105
XceI RCATGY 3 cut(s) 30, 289, 304
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.