MD06G1180300.v1.1

protein At5g50100, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010102
Physical Location & Seq
Forward (+)
31988523 .. 31992360
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1180300.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGCAATGAGGGCAGCTGCTTCAGCATGTAAAATTGGAGGAAGGCAAAGAAACCCTTCTCTCTCTTCAGTTCCTCTCTCTCTCCAACAACTCAAGCTCCATCAAATTCAACCCCAAAGATTCATATCAGCCCCTCCTATTAACCAACCTGGGTTTAAGTCTTCCGTAAGGGCTATAAGTGGGGCAGCTGCAGATCCTTTGACTCCAAAGAAAGAAAATGATCGAGAACAAACACCTCAGAATTGGAAGATTAAAATGCTCTATGACGGGGATTGCCCCTTGTGCATGCGAGAGGTCGATCCTTACCTTCAAACACCCGTGGACATGCTGAGAGAGAGAAATAAGCAATATGGTACCATCAAGTTTGTTGACATAAGTTCAGATGACTACTCGCCAGAGAAGAATCAAGGGCTGGACTACAAGACTGTTATGGGAAACATTCATGCCATCCTCTCTGATGGAACTGTGGTCACTGATGTCGAAGCATTCAGAAAGCTCTACGAGCAAGTGGGGCTCGGATGGGTATATGCCATAACCAAATACGAACCTATTGCAACAATAGCTGGTGCTGTATATGGCGTTTGGGCTAAATATCGTCTTCAAATCACAGGCCGGCCACCCCTAGAAGAGGTTTTGGAGTTGCGAAAGAAGAACAAGGGAGAAGTATGTAAAGATGCAAAAGATTGTAAACTTCCAGAGTAA

Protein Analysis

234

Amino Acids

26.11

Weight (kDa)

8.97

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 87 - 205 2e-21 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 353
AclWI GGATC 2 cut(s) 188, 293
AcoI YGGCCR 1 cut(s) 614
AcsI RAATTY 1 cut(s) 105
AcuI CTGAAG 2 cut(s) 6, 51
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 1 cut(s) 628
AgsI TTSAA 3 cut(s) 110, 311, 602
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 5 cut(s) 17, 97, 188, 496, 563
AluI AGCT 5 cut(s) 17, 97, 188, 496, 563
AlwI GGATC 2 cut(s) 188, 293
AoxI GGCC 2 cut(s) 610, 614
ApeKI GCWGC 4 cut(s) 14, 17, 185, 188
ApoI RAATTY 1 cut(s) 105
Asp700I GAANNNNTTC 1 cut(s) 55
Asp718I GGTACC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 153, 590
BbvI GCAGC 4 cut(s) 4, 26, 175, 197
BccI CCATC 5 cut(s) 108, 365, 452, 455, 513
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 4 cut(s) 15, 18, 186, 189
BlsI GCNGC 4 cut(s) 16, 19, 187, 190
Bme1390I CCNGG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 664
BpiI GAAGAC 2 cut(s) 153, 590
BpuEI CTTGAG 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 124
BsaJI CCNNGG 2 cut(s) 149, 318
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 628
Bse118I RCCGGY 1 cut(s) 612
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 149, 318
BseGI GGATG 2 cut(s) 447, 524
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 628
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 251, 320
BseXI GCAGC 4 cut(s) 4, 26, 175, 197
BshFI GGCC 2 cut(s) 612, 616
BshNI GGYRCC 1 cut(s) 353
BsiSI CCGG 1 cut(s) 613
BslI CCNNNNNNNGG 1 cut(s) 628
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 2 cut(s) 612, 616
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 3 cut(s) 193, 220, 298
BspANI GGCC 2 cut(s) 612, 616
BspCNI CTCAG 2 cut(s) 250, 321
BspLI GGNNCC 1 cut(s) 355
BspMAI CTGCAG 1 cut(s) 193
BspPI GGATC 2 cut(s) 188, 293
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 612
BssAI RCCGGY 1 cut(s) 612
BssECI CCNNGG 2 cut(s) 149, 318
BssMI GATC 3 cut(s) 193, 220, 298
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 427, 466
Bst6I CTCTTC 2 cut(s) 70, 621
BstC8I GCNNGC 2 cut(s) 287, 614
BstDEI CTNAG 2 cut(s) 237, 329
BstDSI CCRYGG 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 2 cut(s) 447, 524
BstKTI GATC 3 cut(s) 196, 223, 301
BstMBI GATC 3 cut(s) 193, 220, 298
BstMWI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 502, 511, 560
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 3 cut(s) 30, 289, 328
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 4, 26, 175, 197
BstV2I GAAGAC 2 cut(s) 153, 590
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 2 cut(s) 612, 616
BtgI CCRYGG 1 cut(s) 318
BtsCI GGATG 2 cut(s) 447, 524
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 2 cut(s) 287, 614
Cfr10I RCCGGY 1 cut(s) 612
Csp6I GTAC 1 cut(s) 354
CviAII CATG 4 cut(s) 27, 286, 325, 443
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 2 cut(s) 237, 329
DpnI GATC 3 cut(s) 195, 222, 300
DpnII GATC 3 cut(s) 193, 220, 298
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 2 cut(s) 70, 621
EarI CTCTTC 2 cut(s) 70, 621
Eco24I GRGCYC 1 cut(s) 516
Eco57I CTGAAG 2 cut(s) 6, 51
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoRII CCWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 516
FaeI CATG 4 cut(s) 30, 289, 328, 446
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FatI CATG 4 cut(s) 26, 285, 324, 442
Fnu4HI GCNGC 4 cut(s) 15, 18, 186, 189
FokI GGATG 2 cut(s) 434, 531
FriOI GRGCYC 1 cut(s) 516
FseI GGCCGGCC 1 cut(s) 616
Fsp4HI GCNGC 4 cut(s) 15, 18, 186, 189
FspBI CTAG 1 cut(s) 623
GluI GCNGC 4 cut(s) 15, 18, 186, 189
HaeIII GGCC 2 cut(s) 612, 616
HapII CCGG 1 cut(s) 613
Hin1II CATG 4 cut(s) 30, 289, 328, 446
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 3 cut(s) 120, 202, 403
HpaII CCGG 1 cut(s) 613
Hpy166II GTNNAC 3 cut(s) 322, 370, 689
Hpy188I TCNGA 5 cut(s) 240, 382, 457, 491, 518
Hpy188III TCNNGA 2 cut(s) 224, 695
Hpy8I GTNNAC 3 cut(s) 322, 370, 689
HpyAV CCTTC 3 cut(s) 36, 66, 317
HpyCH4III ACNGT 2 cut(s) 427, 466
HpyCH4V TGCA 4 cut(s) 191, 285, 554, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 502, 511, 560
HpyF3I CTNAG 2 cut(s) 237, 329
Hsp92II CATG 4 cut(s) 30, 289, 328, 446
KpnI GGTACC 1 cut(s) 357
KroI GCCGGC 1 cut(s) 612
KroNI GCCGGC 1 cut(s) 614
Kzo9I GATC 3 cut(s) 193, 220, 298
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 7 cut(s) 135, 162, 398, 408, 549, 594, 626
Lsp1109I GCAGC 4 cut(s) 4, 26, 175, 197
LweI GCATC 1 cut(s) 664
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 1 cut(s) 469
MalI GATC 3 cut(s) 195, 222, 300
MboI GATC 3 cut(s) 193, 220, 298
MboII GAAGA 7 cut(s) 57, 153, 259, 412, 590, 638, 661
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 3 cut(s) 33, 105, 241
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 3, 32, 84, 144, 246, 286, 461, 622
MroNI GCCGGC 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 3 cut(s) 141, 156, 252
MspA1I CMGCKG 2 cut(s) 17, 188
MspI CCGG 1 cut(s) 613
MspR9I CCNGG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 6 cut(s) 11, 23, 282, 502, 511, 560
NaeI GCCGGC 1 cut(s) 614
NdeII GATC 3 cut(s) 193, 220, 298
NgoMIV GCCGGC 1 cut(s) 612
NlaIII CATG 4 cut(s) 30, 289, 328, 446
NlaIV GGNNCC 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 3 cut(s) 30, 289, 328
PaeI GCATGC 1 cut(s) 289
PctI GAATGC 1 cut(s) 485
PdiI GCCGGC 1 cut(s) 614
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 120, 403
PkrI GCNGC 4 cut(s) 16, 19, 187, 190
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 355
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 17, 188
RigI GGCCGGCC 1 cut(s) 616
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SaqAI TTAA 3 cut(s) 141, 156, 252
SatI GCNGC 4 cut(s) 15, 18, 186, 189
Sau3AI GATC 3 cut(s) 193, 220, 298
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 516
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SphI GCATGC 1 cut(s) 289
Sse9I AATT 3 cut(s) 33, 105, 241
SspMI CTAG 1 cut(s) 623
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 427, 466
TaqI TCGA 3 cut(s) 223, 297, 480
TasI AATT 3 cut(s) 33, 105, 241
TfiI GAWTC 2 cut(s) 120, 403
Tru1I TTAA 3 cut(s) 141, 156, 252
Tru9I TTAA 3 cut(s) 141, 156, 252
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 4 cut(s) 14, 17, 185, 188
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 2 cut(s) 112, 431
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 1 cut(s) 478
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 1 cut(s) 105
XceI RCATGY 3 cut(s) 30, 289, 328
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.