RLG00000005340

protein At5g50100, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010102
Physical Location & Seq
Reverse (-)
66896015 .. 66897880
1866 bp
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UTR
Exon/CDS
Intron
RLM00000005340

Sequence Viewer

Length: 672 bp
ATGGCTTTGAGAGCAGGGTGTAGTAGTATTAGAGGAAGGCAAAGATGTTCATCTCTGTTATCGGTTCCTCGATATCTGCAACTCCAGCCTCATCATCCTCACTCTGCTCCTCTGTGGCTCTCATCATTTCCAATTCATCAACCTGGGTTTAGATTTGGAATGAGAGCTATAAGTGAAGCGGCTGTGAATCCCGTAACTCCCAAGAAAGGAGATGAACGAGAATCAGCACCTGAGAATTGGAAGATTAAGATGCTATATGATGGGGATTGTCCACTATGTATGAGAGAGGTGAATATGCTGAGAGAGAGAAACAAGAGTTATGGTACTATCAAGTTTGTTGACATAAGCTCAGAGGATTATTCTCCAGAGGATAATATGGGGCTAGACTACAAAACTGTTATGGGAAACATTCATGCCATCCTCTCTGATGGAACCGTAGTTACTGATGTCGAAGCATTCAGAAAGTTATATGAGCAAGTTGGGCTCGGATGGGTCTATGCAATAACCAAATATGAACCGATTGGAACAATAGCAGGTGCTGTTTATGGTGTTTGGGCTAAATATCGTCTCCAAATTACAGGCAGACCACCTTTAGAAGAGGTTTTGGAATTGCGGAAGAAGAACAAGGGAGAAGTATGTAATGACAGAACTGATTGTAAACTACCAGAATAA

Protein Analysis

224

Amino Acids

25.22

Weight (kDa)

8.47

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 85 - 195 1.4e-24 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 524
AciI CCGC 2 cut(s) 179, 613
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 1 cut(s) 206
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 2 cut(s) 167, 348
AluI AGCT 2 cut(s) 167, 348
Alw26I GTCTC 1 cut(s) 572
AlwNI CAGNNNCTG 2 cut(s) 230, 539
AsuHPI GGTGA 1 cut(s) 301
BanII GRGCYC 1 cut(s) 486
BccI CCATC 4 cut(s) 254, 422, 425, 483
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 524
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 144
BmiI GGNNCC 2 cut(s) 66, 433
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 240
BpmI CTGGAG 2 cut(s) 68, 348
BsaBI GATNNNNATC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse8I GATNNNNATC 1 cut(s) 49
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 3 cut(s) 94, 417, 494
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 3 cut(s) 222, 290, 363
BseRI GAGGAG 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 572
BsmBI CGTCTC 1 cut(s) 572
BsmI GAATGC 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 486
BspACI CCGC 2 cut(s) 179, 613
BspCNI CTCAG 3 cut(s) 223, 291, 362
BspLI GGNNCC 2 cut(s) 66, 433
BspMI ACCTGC 1 cut(s) 524
BssECI CCNNGG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 397, 436
Bst6I CTCTTC 1 cut(s) 591
BstDEI CTNAG 3 cut(s) 231, 299, 349
BstF5I GGATG 3 cut(s) 94, 417, 494
BstMAI GTCTC 1 cut(s) 572
BstMWI GCNNNNNNNGC 3 cut(s) 11, 85, 481
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BtsCI GGATG 3 cut(s) 94, 417, 494
BveI ACCTGC 1 cut(s) 524
CaiI CAGNNNCTG 2 cut(s) 230, 539
Csp6I GTAC 1 cut(s) 324
CviAII CATG 1 cut(s) 413
CviJI RGCY 9 cut(s) 5, 88, 118, 167, 182, 348, 382, 484, 557
CviKI_1 RGCY 9 cut(s) 5, 88, 118, 167, 182, 348, 382, 484, 557
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 3 cut(s) 231, 299, 349
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 486
Eco32I GATATC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 1 cut(s) 416
FatI CATG 1 cut(s) 412
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 3 cut(s) 81, 404, 501
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 1 cut(s) 383
GluI GCNGC 1 cut(s) 180
GsuI CTGGAG 2 cut(s) 68, 348
Hin1II CATG 1 cut(s) 416
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 2 cut(s) 187, 221
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 272, 340, 659
Hpy188I TCNGA 4 cut(s) 352, 427, 461, 488
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 3 cut(s) 272, 340, 659
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 2 cut(s) 397, 436
HpyCH4V TGCA 2 cut(s) 79, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 85, 481
HpyF3I CTNAG 3 cut(s) 231, 299, 349
Hsp92II CATG 1 cut(s) 416
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 7 cut(s) 98, 129, 156, 243, 378, 519, 564
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 383
MaeIII GTNAC 2 cut(s) 193, 439
MboII GAAGA 4 cut(s) 253, 608, 628, 631
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 4 cut(s) 132, 235, 573, 608
MseI TTAA 1 cut(s) 246
MspR9I CCNGG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 11, 85, 481
NlaIII CATG 1 cut(s) 416
NlaIV GGNNCC 2 cut(s) 66, 433
PaqCI CACCTGC 1 cut(s) 524
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 2 cut(s) 187, 221
PkrI GCNGC 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 66, 433
PsrI GAACNNNNNNTAC 2 cut(s) 424, 456
PstNI CAGNNNCTG 2 cut(s) 230, 539
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 180
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 486
SetI ASST 8 cut(s) 145, 169, 232, 291, 350, 538, 592, 603
SfaNI GCATC 1 cut(s) 240
Sse9I AATT 4 cut(s) 132, 235, 573, 608
SsiI CCGC 2 cut(s) 179, 613
SspMI CTAG 1 cut(s) 383
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 397, 436
TaqI TCGA 2 cut(s) 70, 450
TasI AATT 4 cut(s) 132, 235, 573, 608
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 187, 221
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TspDTI ATGAA 5 cut(s) 39, 125, 228, 401, 528
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.