Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Reverse (-)

56325281 .. 56331397

Gene Structure 6117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0426500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGGAAGAGAAGATGAGAATTGGAGGAGGGTTGCTTAGTGGAGCACAGTCTAGTATACTTGAGGAGATGAAGTTGTTAAAAGAACTGCAGGATCATTCTGGGTCCCGGAAGGCCATAAATTCCGAGTTATGGCATGCCTGCGCCGGCCCCCTTGTTTGTTTGCCTCAAGTGGGGAGTCTTGCTTATTACTTCCCTCAGGGCCACAGCGAGCAGGTGGCAGTTTCCACAAAGAGAATGGCAACCTCGCAAATTCCCAACTATCCGAATCTCCCGTCTCAGTTGCTATGCCAAGTTCAAAATGTTACGCTACACGCAGACAAAGAAACCGATGAGATCTTCACACAAATGTGTCTTAAACCAGTCAATTCTGAAAAAGATGTTTTCCCTGTACCAGACTTTGGACTGAAGCCTAGCAAGCATCCAAGTGAGTTTTTCTGCAAAATTTTGACTGCAAGTGATACAAGCACACATGGTGGTTTCTCTGTGCCGCGGAGGGCAGCAGAAAAGCTCTTTCCTCCCTTGGATTTCACAATGCAACCGCCAACCCAAGAACTTGTTGTCCGAGACTTGCATGATAATACCTGGACATTTCGCCACATTTACCGCGGCCAACCGAAGCGCCACCTTCTCACAACTGGTTGGAGTTTGTTTGTTGGCACAAAGAGGCTTAGAGCAGGCGATTCTGTGCTATTTATCAGGGATGAGAAGTCACAGCTATTGGTTGGCGTAAGGCGAGCAAATCGTCAGCAGACCACATTACCATCCTCAGTTCTATCTGCTGATAGCATGCATATCGGTGTCCTTGCTGCTGCAGCTCATGCTGCTGCTAATCGAAGTCCATTCACTATATTCTACAATCCAAGGGCATGTCCTTCAGAATTTGTTATACCTTTGGTCAAATTCCAAAAGGCTCTATATGGGACCCAACTGTCTGTTGGCATGAGGTTTGGAATGATGTTTGAGACAGAGGAGTCCGGCAAGCGTAGATACATGGGTACAATAGTTAATATTAGTGATTTCGATCCACTGAGATGGCCTGGTTCCAAGTGGCGAAATCTTCAGGTAGAGTGGGACGAGCCGGGGTGTTGCGATAAACAGAATAGGGTCAGCTCATGGGAAGTTGAGACTCCTGAAAGTCTTTTCATATTTCCTTCCCTGACTTCGAGTCTTAAACGTCCATTTCACTCTGGATACTTGGAAACTGAGTGGGCCAATATGATTAAAAGACCTTTTATCCGGGTTCCTGAGATTGGAAACATGAACTCTTTTCCTTATCCGATGTCAAATCTATGTTCAGAACAGCTAGTTAATATGCTACTAAAACCTCAAGTTAGCCAGGCTGGAACATTATCTGCTCTACAACAGGAATCGGCTGCTAATGGAGGTCCACTAGAAGATATGCAGGCCATGCAGGCGAAAATGAACCAAAAGAATCTGGTTTACTGTTCTGAAGGTATGTCTCTGCAGAGTCAAAACCCTTCCCAGTTGAACACCACAGCTAAGTTTGGAAGTCAGACACCAGTTGGAGTTAACACTGACAAAACGAAGTTAGAACCTGATCTTTCAACTGATCAGTTAAGCCAGTTAAGCTCTTCAGGACAGGGCAATGAGGAAAAATTGGCTGCTGGGATTGCTGGTAGCCCTTATAACAATGCATGCATCAACCAGAACCAGGGCCAATTACAAGCTAGCCCACGGCCTATGCAGCAACCTATGGAATCATTACTCTACCACTCCCAACAAACTGATCTACCTCAGTCAGATTTTAATAGTGCAAATGGTTCACTTCCATCCTTAGACAATGATGAATGCATGTTCTACCAACCCTTTGCTGGGATACTGAGATCACCAGGGCCTTTGTCTGCATATGGGTTGCAGGACTCTCCATCTGTTTTAACTGAAGCAAATAATTTCTCCCCAACTTCAATGGGTCAGGAAATGTGGGATAATAGCCTCAGTAGGCTTTTACCGCAAGTTGATCAGCTCACTTCATCCCATCAAGTCCCATCTGCCTTTAATTCTATTCCCAACTCGGGCAGTCTGAGAGATTTGTCCGATGAGAGCAACAACCAAAGTGGGGTTTATGGTTGCCCCTCTGTTGATGTTGGTACTGGTGTGAGTAATATTGTTGCTGACCCTTCTGTTACAAGCACCATAATGGATGAGTTCTCTAAACTAAAGCATGCCGACTTCCAGAATCCTTCCGAATGTTTGGTTGGCAACTTGAGCTCGAGTCAGGATCTGCAGTCCCAGATTACCTCTGCAAGCCTAGGGGATTCTCACGCTTTGTCCAGGCAAGAACTGGCAGACAACTCCGGTGGTACATCCTCAAGCAATGTTGATCTTGATGAGAGCAGTCTACTGCAGAATAGCTGGCACCAAGTGGTCCCACCCGTGCGAACTTACACCAAGGTTCAAAAGGCGGGATCTGTTGGTAGATCAATTGATGTTACTAGTTACACAAACTATGAGGAACTATGTTCTGCAATTGAGTGCATGTTTGGACTGGAAGGGCTGCTTAACGACCCAAGAGGTTCAGGGTGGAAACTAGTTTATGTGGACTATGAGAACGATGTTCTACTTGTCGGGGATGATCCTTGGGAGGAATTTGTGGGGTGTGTGCGCTGCATCAGAATCCTTTCGCCAACAGAAGTTCAGCAGATGAGTGAAGAGGGAATGAAGCTTCTCAACAGCGCTGCTGCTTTGCAAGGGATGAACACCACCGTGTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006950 GO:0008150 GO:0008152 GO:0009611 GO:0009888 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0031099 GO:0032502 GO:0040007 GO:0042060 GO:0042246 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048589 GO:0048856 GO:0050896 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF393

Protein Analysis

910

Amino Acids

100.06

Weight (kDa)

5.21

Isoelectric Point (pI)

57.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	145 - 246	1.2e-21	B3 DNA binding domain
ARF_AD	PF06507	294 - 376	7.4e-32	Auxin response factor ancillary domain
AUX_IAA	PF02309	798 - 886	7.5e-10	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1647
AarI	CACCTGC	1 cut(s)	202
AasI	GACNNNNNNGTC	2 cut(s)	928, 970
Acc36I	ACCTGC	1 cut(s)	202
AccB1I	GGYRCC	1 cut(s)	2376
AccB7I	CCANNNNNTGG	1 cut(s)	398
AccI	GTMKAC	2 cut(s)	55, 2359
AccII	CGCG	2 cut(s)	490, 606
AciI	CCGC	7 cut(s)	488, 490, 539, 604, 606, 1970, 2423
AclWI	GGATC	5 cut(s)	99, 1016, 2247, 2434, 2588
AcoI	YGGCCR	1 cut(s)	607
AcsI	RAATTY	6 cut(s)	118, 249, 441, 878, 899, 2606
AcuI	CTGAAG	6 cut(s)	425, 858, 1043, 1470, 1578, 1920
AdeI	CACNNNGTG	2 cut(s)	473, 2383
AfaI	GTAC	4 cut(s)	390, 997, 2110, 2323
AfeI	AGCGCT	1 cut(s)	2695
AfiI	CCNNNNNNNGG	9 cut(s)	129, 170, 398, 1250, 1672, 1832, 1833, 2033, 2077
AgsI	TTSAA	5 cut(s)	296, 1489, 1566, 1926, 2417
AhdI	GACNNNNNGTC	1 cut(s)	1164
AhlI	ACTAGT	2 cut(s)	2453, 2548
AjnI	CCWGG	6 cut(s)	581, 1036, 1335, 1671, 1849, 2291
AjuI	GAANNNNNNNTTGG	2 cut(s)	2199, 2231
AleI	CACNNNNGTG	2 cut(s)	346, 2723
AloI	GAACNNNNNNTCC	2 cut(s)	543, 575
Alw21I	GWGCWC	2 cut(s)	46, 2231
Alw26I	GTCTC	5 cut(s)	279, 558, 956, 1118, 1464
AlwI	GGATC	5 cut(s)	99, 1016, 2247, 2434, 2588
AlwNI	CAGNNNCTG	1 cut(s)	2242
Ama87I	CYCGRG	2 cut(s)	2032, 2230
Aor51HI	AGCGCT	1 cut(s)	2695
ApoI	RAATTY	6 cut(s)	118, 249, 441, 878, 899, 2606
ArsI	GACNNNNNNTTYG	2 cut(s)	257, 289
Asp700I	GAANNNNTTC	1 cut(s)	2638
AspA2I	CCTAGG	1 cut(s)	2269
AspLEI	GCGC	4 cut(s)	143, 621, 2625, 2696
AspS9I	GGNCC	9 cut(s)	102, 146, 199, 921, 1209, 1385, 1675, 1853, 2386
AsuC2I	CCSGG	3 cut(s)	106, 1080, 1238
AsuHPI	GGTGA	1 cut(s)	1839
AsuNHI	GCTAGC	1 cut(s)	1688
AvaI	CYCGRG	2 cut(s)	2032, 2230
AvaII	GGWCC	4 cut(s)	102, 921, 1385, 2386
AvrII	CCTAGG	1 cut(s)	2269
AxyI	CCTNAGG	1 cut(s)	195
BaeI	ACNNNNGTAYC	6 cut(s)	979, 987, 1012, 1020, 2100, 2133
BanI	GGYRCC	1 cut(s)	2376
BanII	GRGCYC	1 cut(s)	2231
Bbv12I	GWGCWC	2 cut(s)	46, 2231
BccI	CCATC	6 cut(s)	769, 1026, 1798, 1894, 2004, 2014
BceAI	ACGGC	1 cut(s)	1712
BcgI	CGANNNNNNTGC	2 cut(s)	775, 809
BciT130I	CCWGG	6 cut(s)	583, 1038, 1337, 1673, 1851, 2293
BciVI	GTATCC	2 cut(s)	1184, 1830
BclI	TGATCA	2 cut(s)	1570, 1978
BcnI	CCSGG	3 cut(s)	106, 1080, 1238
BcoDI	GTCTC	5 cut(s)	279, 558, 956, 1118, 1464
BcuI	ACTAGT	2 cut(s)	2453, 2548
BfaI	CTAG	8 cut(s)	51, 411, 1304, 1391, 1689, 2270, 2454, 2549
BfmI	CTRYAG	5 cut(s)	86, 810, 1463, 2243, 2363
BfoI	RGCGCY	2 cut(s)	622, 2697
BfuAI	ACCTGC	1 cut(s)	202
BfuI	GTATCC	2 cut(s)	1184, 1830
BglI	GCCNNNNNGGC	1 cut(s)	1412
BglII	AGATCT	1 cut(s)	333
BlnI	CCTAGG	1 cut(s)	2269
Bme1390I	CCNGG	9 cut(s)	106, 583, 1038, 1080, 1238, 1337, 1673, 1851, 2293
Bme18I	GGWCC	4 cut(s)	102, 921, 1385, 2386
BmeRI	GACNNNNNGTC	1 cut(s)	1164
BmeT110I	CYCGRG	2 cut(s)	2032, 2230
BmgT120I	GGNCC	9 cut(s)	102, 146, 199, 921, 1209, 1385, 1675, 1853, 2386
BmiI	GGNNCC	9 cut(s)	103, 104, 148, 922, 923, 1042, 1242, 2378, 2388
BmrFI	CCNGG	9 cut(s)	106, 583, 1038, 1080, 1238, 1337, 1673, 1851, 2293
BmrI	ACTGGG	1 cut(s)	1477
BmsI	GCATC	3 cut(s)	427, 1668, 2637
BmtI	GCTAGC	1 cut(s)	1692
BmuI	ACTGGG	1 cut(s)	1477
BpuEI	CTTGAG	5 cut(s)	80, 150, 1311, 2245, 2314
BpuMI	CCSGG	3 cut(s)	106, 1080, 1238
BsaBI	GATNNNNATC	1 cut(s)	1020
BsaWI	WCCGGW	1 cut(s)	2315
BsaXI	ACNNNNNCTCC	2 cut(s)	56, 86
Bsc4I	CCNNNNNNNGG	9 cut(s)	129, 170, 398, 1250, 1672, 1832, 1833, 2033, 2077
Bse118I	RCCGGY	1 cut(s)	143
Bse1I	ACTGG	8 cut(s)	359, 640, 1483, 1520, 1582, 2116, 2307, 2511
Bse21I	CCTNAGG	1 cut(s)	195
Bse3DI	GCAATG	2 cut(s)	1612, 2341
Bse8I	GATNNNNATC	1 cut(s)	1020
BseBI	CCWGG	6 cut(s)	583, 1038, 1337, 1673, 1851, 2293
BseGI	GGATG	9 cut(s)	418, 706, 761, 1790, 1991, 2167, 2324, 2596, 2718
BseJI	GATNNNNATC	1 cut(s)	1020
BseLI	CCNNNNNNNGG	9 cut(s)	129, 170, 398, 1250, 1672, 1832, 1833, 2033, 2077
BseMI	GCAATG	2 cut(s)	1612, 2341
BseNI	ACTGG	8 cut(s)	359, 640, 1483, 1520, 1582, 2116, 2307, 2511
BseRI	GAGGAG	3 cut(s)	39, 77, 983
BseYI	CCCAGC	2 cut(s)	1625, 1832
Bsh1236I	CGCG	2 cut(s)	490, 606
BshNI	GGYRCC	1 cut(s)	2376
BsiHKAI	GWGCWC	2 cut(s)	46, 2231
BsiHKCI	CYCGRG	2 cut(s)	2032, 2230
BsiSI	CCGG	6 cut(s)	106, 144, 975, 1079, 1237, 2316
BslFI	GGGAC	6 cut(s)	88, 934, 1085, 1988, 2233, 2372
BslI	CCNNNNNNNGG	9 cut(s)	129, 170, 398, 1250, 1672, 1832, 1833, 2033, 2077
BsmAI	GTCTC	5 cut(s)	279, 558, 956, 1118, 1464
BsmBI	CGTCTC	1 cut(s)	279
BsmFI	GGGAC	6 cut(s)	88, 934, 1085, 1988, 2233, 2372
BsmI	GAATGC	1 cut(s)	1814
BsoBI	CYCGRG	2 cut(s)	2032, 2230
Bsp1286I	GDGCHC	2 cut(s)	46, 2231
BspACI	CCGC	7 cut(s)	488, 490, 539, 604, 606, 1970, 2423
BspFNI	CGCG	2 cut(s)	490, 606
BspLI	GGNNCC	9 cut(s)	103, 104, 148, 922, 923, 1042, 1242, 2378, 2388
BspMAI	CTGCAG	5 cut(s)	90, 814, 1467, 2247, 2367
BspMI	ACCTGC	1 cut(s)	202
BspOI	GCTAGC	1 cut(s)	1692
BspPI	GGATC	5 cut(s)	99, 1016, 2247, 2434, 2588
BspQI	GCTCTTC	1 cut(s)	1597
BspT107I	GGYRCC	1 cut(s)	2376
BsrDI	GCAATG	2 cut(s)	1612, 2341
BsrFI	RCCGGY	1 cut(s)	143
BsrI	ACTGG	8 cut(s)	359, 640, 1483, 1520, 1582, 2116, 2307, 2511
BssAI	RCCGGY	1 cut(s)	143
BssNAI	GTATAC	1 cut(s)	56
BssT1I	CCWWGG	5 cut(s)	519, 860, 2269, 2409, 2597
Bst1107I	GTATAC	1 cut(s)	56
Bst2UI	CCWGG	6 cut(s)	583, 1038, 1337, 1673, 1851, 2293
Bst4CI	ACNGT	4 cut(s)	48, 930, 1445, 2725
Bst6I	CTCTTC	2 cut(s)	1597, 2664
BstAPI	GCANNNNNTGC	2 cut(s)	818, 1408
BstDSI	CCRYGG	3 cut(s)	488, 604, 1694
BstF5I	GGATG	9 cut(s)	418, 706, 761, 1790, 1991, 2167, 2324, 2596, 2718
BstFNI	CGCG	2 cut(s)	490, 606
BstH2I	RGCGCY	2 cut(s)	622, 2697
BstHHI	GCGC	4 cut(s)	143, 621, 2625, 2696
BstMAI	GTCTC	5 cut(s)	279, 558, 956, 1118, 1464
BstNI	CCWGG	6 cut(s)	583, 1038, 1337, 1673, 1851, 2293
BstNSI	RCATGY	7 cut(s)	137, 790, 870, 1659, 1816, 2186, 2500
BstSCI	CCNGG	9 cut(s)	104, 581, 1036, 1078, 1236, 1335, 1671, 1849, 2291
BstSFI	CTRYAG	5 cut(s)	86, 810, 1463, 2243, 2363
BstUI	CGCG	2 cut(s)	490, 606
BstX2I	RGATCY	3 cut(s)	333, 2239, 2426
BstXI	CCANNNNNNTGG	1 cut(s)	1032
BstYI	RGATCY	3 cut(s)	333, 2239, 2426
BstZ17I	GTATAC	1 cut(s)	56
Bsu36I	CCTNAGG	1 cut(s)	195
BsuI	GTATCC	2 cut(s)	1184, 1830
BtgI	CCRYGG	3 cut(s)	488, 604, 1694
BtsCI	GGATG	9 cut(s)	418, 706, 761, 1790, 1991, 2167, 2324, 2596, 2718
BtsIMutI	CAGTG	2 cut(s)	1025, 1533
BveI	ACCTGC	1 cut(s)	202
CaiI	CAGNNNCTG	1 cut(s)	2242
CfoI	GCGC	4 cut(s)	143, 621, 2625, 2696
Cfr10I	RCCGGY	1 cut(s)	143
Cfr13I	GGNCC	9 cut(s)	102, 146, 199, 921, 1209, 1385, 1675, 1853, 2386
Cfr42I	CCGCGG	2 cut(s)	491, 607
Csp6I	GTAC	4 cut(s)	389, 996, 2109, 2322
CspCI	CAANNNNNGTGG	4 cut(s)	2056, 2091, 2299, 2334
CviQI	GTAC	4 cut(s)	389, 996, 2109, 2322
DraIII	CACNNNGTG	2 cut(s)	473, 2383
DrdI	GACNNNNNNGTC	2 cut(s)	928, 970
DriI	GACNNNNNGTC	1 cut(s)	1164
DseDI	GACNNNNNNGTC	2 cut(s)	928, 970
EaeI	YGGCCR	1 cut(s)	607
Eam1104I	CTCTTC	2 cut(s)	1597, 2664
Eam1105I	GACNNNNNGTC	1 cut(s)	1164
EarI	CTCTTC	2 cut(s)	1597, 2664
Ecl136II	GAGCTC	1 cut(s)	2229
Eco130I	CCWWGG	5 cut(s)	519, 860, 2269, 2409, 2597
Eco24I	GRGCYC	1 cut(s)	2231
Eco47I	GGWCC	4 cut(s)	102, 921, 1385, 2386
Eco47III	AGCGCT	1 cut(s)	2695
Eco53kI	GAGCTC	1 cut(s)	2229
Eco57I	CTGAAG	6 cut(s)	425, 858, 1043, 1470, 1578, 1920
Eco81I	CCTNAGG	1 cut(s)	195
Eco88I	CYCGRG	2 cut(s)	2032, 2230
EcoICRI	GAGCTC	1 cut(s)	2229
EcoO109I	RGGNCCY	3 cut(s)	102, 921, 1853
EcoRII	CCWGG	6 cut(s)	581, 1036, 1335, 1671, 1849, 2291
EcoT14I	CCWWGG	5 cut(s)	519, 860, 2269, 2409, 2597
EcoT22I	ATGCAT	4 cut(s)	792, 1657, 1661, 1814
EcoT38I	GRGCYC	1 cut(s)	2231
ErhI	CCWWGG	5 cut(s)	519, 860, 2269, 2409, 2597
Esp3I	CGTCTC	1 cut(s)	279
FalI	AAGNNNNNCTT	2 cut(s)	2502, 2534
FaqI	GGGAC	6 cut(s)	88, 934, 1085, 1988, 2233, 2372
FauI	CCCGC	1 cut(s)	2416
FauNDI	CATATG	1 cut(s)	1867
FbaI	TGATCA	2 cut(s)	1570, 1978
FblI	GTMKAC	2 cut(s)	55, 2359
FokI	GGATG	9 cut(s)	405, 713, 748, 1777, 1978, 2174, 2311, 2603, 2725
FriOI	GRGCYC	1 cut(s)	2231
FspBI	CTAG	8 cut(s)	51, 411, 1304, 1391, 1689, 2270, 2454, 2549
GlaI	GCGC	4 cut(s)	142, 620, 2624, 2695
GsaI	CCCAGC	2 cut(s)	1629, 1836
HaeII	RGCGCY	2 cut(s)	622, 2697
HapII	CCGG	6 cut(s)	106, 144, 975, 1079, 1237, 2316
HhaI	GCGC	4 cut(s)	143, 621, 2625, 2696
Hin6I	GCGC	4 cut(s)	141, 619, 2623, 2694
HinP1I	GCGC	4 cut(s)	141, 619, 2623, 2694
HincII	GTYRAC	1 cut(s)	1531
HindII	GTYRAC	1 cut(s)	1531
HindIII	AAGCTT	1 cut(s)	2681
HpaI	GTTAAC	1 cut(s)	1531
HpaII	CCGG	6 cut(s)	106, 144, 975, 1079, 1237, 2316
HphI	GGTGA	1 cut(s)	1839
Hpy166II	GTNNAC	7 cut(s)	56, 1388, 1441, 1531, 1784, 2360, 2560
Hpy188III	TCNNGA	8 cut(s)	1130, 1188, 1244, 1596, 1934, 2194, 2237, 2345
Hpy8I	GTNNAC	7 cut(s)	56, 1388, 1441, 1531, 1784, 2360, 2560
HpyAV	CCTTC	9 cut(s)	103, 635, 882, 1161, 1445, 1488, 2148, 2211, 2504
HpyCH4III	ACNGT	4 cut(s)	48, 930, 1445, 2725
HpyCH4IV	ACGT	1 cut(s)	1174
HpySE526I	ACGT	1 cut(s)	1174
HspAI	GCGC	4 cut(s)	141, 619, 2623, 2694
KflI	GGGWCCC	2 cut(s)	102, 921
KroI	GCCGGC	1 cut(s)	143
KroNI	GCCGGC	1 cut(s)	145
Ksp22I	TGATCA	2 cut(s)	1570, 1978
KspAI	GTTAAC	1 cut(s)	1531
KspI	CCGCGG	2 cut(s)	491, 607
LguI	GCTCTTC	1 cut(s)	1597
LmnI	GCTCC	1 cut(s)	41
LweI	GCATC	3 cut(s)	427, 1668, 2637
MaeI	CTAG	8 cut(s)	51, 411, 1304, 1391, 1689, 2270, 2454, 2549
MaeII	ACGT	1 cut(s)	1174
MaeIII	GTNAC	5 cut(s)	301, 708, 2143, 2449, 2456
MboII	GAAGA	7 cut(s)	17, 22, 328, 1049, 1406, 1584, 2681
MfeI	CAATTG	2 cut(s)	2442, 2487
MflI	RGATCY	3 cut(s)	333, 2239, 2426
MhlI	GDGCHC	2 cut(s)	46, 2231
MlyI	GAGTC	7 cut(s)	184, 980, 1120, 1174, 1477, 1874, 2242
MmeI	TCCRAC	2 cut(s)	620, 1504
Mph1103I	ATGCAT	4 cut(s)	792, 1657, 1661, 1814
MroNI	GCCGGC	1 cut(s)	143
MroXI	GAANNNNTTC	1 cut(s)	2638
MslI	CAYNNNNRTG	7 cut(s)	344, 346, 423, 795, 1030, 2156, 2723
MspA1I	CMGCKG	2 cut(s)	490, 606
MspI	CCGG	6 cut(s)	106, 144, 975, 1079, 1237, 2316
MspR9I	CCNGG	9 cut(s)	106, 583, 1038, 1080, 1238, 1337, 1673, 1851, 2293
MunI	CAATTG	2 cut(s)	2442, 2487
Mva1269I	GAATGC	1 cut(s)	1814
MvaI	CCWGG	6 cut(s)	583, 1038, 1337, 1673, 1851, 2293
MvnI	CGCG	2 cut(s)	490, 606
NaeI	GCCGGC	1 cut(s)	145
NciI	CCSGG	3 cut(s)	106, 1080, 1238
NdeI	CATATG	1 cut(s)	1867
NgoMIV	GCCGGC	1 cut(s)	143
NheI	GCTAGC	1 cut(s)	1688
NlaIV	GGNNCC	9 cut(s)	103, 104, 148, 922, 923, 1042, 1242, 2378, 2388
NmuCI	GTSAC	1 cut(s)	708
NsiI	ATGCAT	4 cut(s)	792, 1657, 1661, 1814
NspI	RCATGY	7 cut(s)	137, 790, 870, 1659, 1816, 2186, 2500
OliI	CACNNNNGTG	2 cut(s)	346, 2723
PaeI	GCATGC	4 cut(s)	137, 790, 1659, 2186
PaeR7I	CTCGAG	1 cut(s)	2230
PaqCI	CACCTGC	1 cut(s)	202
PciSI	GCTCTTC	1 cut(s)	1597
PctI	GAATGC	1 cut(s)	1814
PdiI	GCCGGC	1 cut(s)	145
PdmI	GAANNNNTTC	1 cut(s)	2638
PfeI	GAWTC	8 cut(s)	265, 680, 1367, 1432, 1718, 2197, 2276, 2634
PflMI	CCANNNNNTGG	1 cut(s)	398
PfoI	TCCNGGA	1 cut(s)	104
PleI	GAGTC	7 cut(s)	183, 979, 1120, 1173, 1476, 1874, 2241
PpsI	GAGTC	7 cut(s)	183, 979, 1120, 1173, 1476, 1874, 2241
PpuMI	RGGWCCY	2 cut(s)	102, 921
PsiI	TTATAA	1 cut(s)	1647
Psp124BI	GAGCTC	1 cut(s)	2231
Psp5II	RGGWCCY	2 cut(s)	102, 921
Psp6I	CCWGG	6 cut(s)	581, 1036, 1335, 1671, 1849, 2291
PspFI	CCCAGC	2 cut(s)	1625, 1832
PspGI	CCWGG	6 cut(s)	581, 1036, 1335, 1671, 1849, 2291
PspN4I	GGNNCC	9 cut(s)	103, 104, 148, 922, 923, 1042, 1242, 2378, 2388
PspPI	GGNCC	9 cut(s)	102, 146, 199, 921, 1209, 1385, 1675, 1853, 2386
PspPPI	RGGWCCY	2 cut(s)	102, 921
PspXI	VCTCGAGB	1 cut(s)	2230
PstI	CTGCAG	5 cut(s)	90, 814, 1467, 2247, 2367
PstNI	CAGNNNCTG	1 cut(s)	2242
PsuI	RGATCY	3 cut(s)	333, 2239, 2426
RsaI	GTAC	4 cut(s)	390, 997, 2110, 2323
RsaNI	GTAC	4 cut(s)	389, 996, 2109, 2322
RseI	CAYNNNNRTG	7 cut(s)	344, 346, 423, 795, 1030, 2156, 2723
SacI	GAGCTC	1 cut(s)	2231
SacII	CCGCGG	2 cut(s)	491, 607
SapI	GCTCTTC	1 cut(s)	1597
Sau96I	GGNCC	9 cut(s)	102, 146, 199, 921, 1209, 1385, 1675, 1853, 2386
SchI	GAGTC	7 cut(s)	184, 980, 1120, 1174, 1477, 1874, 2242
ScrFI	CCNGG	9 cut(s)	106, 583, 1038, 1080, 1238, 1337, 1673, 1851, 2293
SduI	GDGCHC	2 cut(s)	46, 2231
SfaNI	GCATC	3 cut(s)	427, 1668, 2637
SfcI	CTRYAG	5 cut(s)	86, 810, 1463, 2243, 2363
Sfr274I	CTCGAG	1 cut(s)	2230
Sfr303I	CCGCGG	2 cut(s)	491, 607
SgrBI	CCGCGG	2 cut(s)	491, 607
SinI	GGWCC	4 cut(s)	102, 921, 1385, 2386
SlaI	CTCGAG	1 cut(s)	2230
SmiMI	CAYNNNNRTG	7 cut(s)	344, 346, 423, 795, 1030, 2156, 2723
SmlI	CTYRAG	6 cut(s)	59, 165, 1326, 2224, 2230, 2329
SmoI	CTYRAG	6 cut(s)	59, 165, 1326, 2224, 2230, 2329
SpeI	ACTAGT	2 cut(s)	2453, 2548
SphI	GCATGC	4 cut(s)	137, 790, 1659, 2186
SsiI	CCGC	7 cut(s)	488, 490, 539, 604, 606, 1970, 2423
SspI	AATATT	2 cut(s)	1009, 2125
SspMI	CTAG	8 cut(s)	51, 411, 1304, 1391, 1689, 2270, 2454, 2549
SstI	GAGCTC	1 cut(s)	2231
StyD4I	CCNGG	9 cut(s)	104, 581, 1036, 1078, 1236, 1335, 1671, 1849, 2291
StyI	CCWWGG	5 cut(s)	519, 860, 2269, 2409, 2597
TaaI	ACNGT	4 cut(s)	48, 930, 1445, 2725
TaiI	ACGT	1 cut(s)	1177
TaqI	TCGA	4 cut(s)	832, 1020, 1163, 2231
TauI	GCSGC	2 cut(s)	490, 609
TfiI	GAWTC	8 cut(s)	265, 680, 1367, 1432, 1718, 2197, 2276, 2634
TscAI	CASTG	2 cut(s)	1032, 1540
TseFI	GTSAC	1 cut(s)	708
Tsp45I	GTSAC	1 cut(s)	708
TspDTI	ATGAA	8 cut(s)	83, 1132, 1274, 1436, 1821, 1980, 2693, 2729
TspRI	CASTG	2 cut(s)	1032, 1540
Van91I	CCANNNNNTGG	1 cut(s)	398
VpaK11BI	GGWCC	4 cut(s)	102, 921, 1385, 2386
XapI	RAATTY	6 cut(s)	118, 249, 441, 878, 899, 2606
XceI	RCATGY	7 cut(s)	137, 790, 870, 1659, 1816, 2186, 2500
XcmI	CCANNNNNNNNNTGG	4 cut(s)	232, 932, 1829, 2299
XhoI	CTCGAG	1 cut(s)	2230
XmaJI	CCTAGG	1 cut(s)	2269
XmiI	GTMKAC	2 cut(s)	55, 2359
XmnI	GAANNNNTTC	1 cut(s)	2638
XspI	CTAG	8 cut(s)	51, 411, 1304, 1391, 1689, 2270, 2454, 2549
Zsp2I	ATGCAT	4 cut(s)	792, 1657, 1661, 1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.