Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Reverse (-)

80417470 .. 80421109

Gene Structure 3640 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00058510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGAGGTGTTGATCCCAGCTTTCTCAGTTTTGTTCATCATAATTGGTACTTTAGGAGGCTACTTCTTCGGAAGAGCCAGAGGTCGAAAAGATCGAATCGCAGTACTCTCCGATCAGCCACAACCTGTAACTTCCGTGGTGGACCGGCCTAAAAACGATGTGCAACAACCACCGGTCACCGCCATGGTTTCCCAACCTAAAGACGAGGCCACCATGGAGTCCATTATCCCCAAAAGAATCAAAATTTTCGAGTCCATCCAGGCCCAACGAAAGGCCCACATTCAGTCTCTTCCCCGGAATCCATCAAGTGAAGTTGAAAAGTTCATGTCATTTCATGCTCGGAAACATAGTAGAAGTTTGCTTTTGATTACATTGCGGGATGGGCAGGTGATGGAGGGGAAGAAGTGGATAACTTCCCCTTTGGAAATTGCTGAGGAGATTTCGAAGAGTTTGGCTTCGAAAGCTTTGATTGCTGAGGTCAGTGGGACTCTATGGGACATGGCAAGGCCTTTGGAGCGTGACTGTGAGCTTAAGATATTCACATTTGAAGACTACAAAGGCCGTGACACCTTCTGGCACTCTAGTGCTCATATTCTTGGACAGGCACTTGAGGCCAAGTATGGATGCAAGCTCTGTATTGGTCCCTGCACAACAAAAGAAGAGGGGTTCTACTATGATGCATACTATGGCAGTTTGGGCTTCAATGATGAGCACTTAAAGAAAATTGAATCAGAGGTAGAGAAGGCTGTCAAGGCCAAACAGTGCTTTGAGCGCATTGAAGTATCAATGGAACAGGCCCTCGAGATTTTCTCTGATAACAAATTCAGGATTGAGATCATCAATGAGCTTCCTATAGATAAGGCTATAACTGTATACAGATGTGGCAACTTGGTGGACTTGTGTCATGGACCTCATATCCCAAATACATCCTTTGTCAAAGCATTTAGATGTTTGAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGCGAAAGTCTGCAGCGAGTTTATGGAATATCATATCCGGATAAAAAACTTTTGCAGGCATATATCAAGCAGCAGGAAGAAGCAAAAAAATATGACCATAGGTTGTTGGGTAAATCACAGGAGCTCATATTTTGTCACCCATATAGCCCGGGAAGTTGGTTCTTCACCCCCAACGGAGCTCGGATTTATAAGAAATTGATGCAGTTCATGGAAAATGAATATTTGGAAAGAGGATATCAGGAGGTGATAACACCAAACATATTCAAAATGGATTTGTTTGAAAAATCCAAGCATGCAGGACACTACAAACAGAATATGTTTCTTTTAGAGATTGAGAAAGAAGAATATGGTTTGAAGCCAATGAACTGTCCTGGTCACTGTGAAGAGTTTCGACATAGGCCTCGTTCTTATAGAGAACTTCCTCTTCGGATGGCTGAATTTGGGGTGCTGCATCGAAATGAGGTCAGTGGTGCACTAGATGGATTGACTCGTGTCAGGAGATTCGTGCAGGATGATGCTCATATATTTTGTAGGGAGTCCCAACTGAAAGATGAACTCAAGGGTGTCCTCGACTTCATCAAGTATACCTATGACATCTTTGGTCTCTCTTTTGATGTGGAGCTATCAACAAGGCCAGAAAGTTACCATGGAGAACGAGCAACATGGGATAAAGCCGAGGCAATTCTTACAGAAGCACTAAATGAGTTTGGCAAGCCTTGGCAGATAAATGAAGGGGATGGGGCATTTTATGGACCAAAGATAGATTTCGGTTTCGACCTACATTACAATTCAGAGGGTAAAGAAGGCAAGAGGGAGAGGCCTGTTATGATACACAGAGCTGTATTCGGATCCTTTGAGCGTTTCTTTGGTATACTTTTGGAGCACTACAAAGGAAAATGGCCCTTCTGGCTTAGTCCACGTCAGGCAATTGTTTGCCCCGTCTCAGAGAAATCCGAGGCTTATGCTCTACAGGTTCAGGAACGGATTCGTTGCGCTGGTTATCACGTTGATGTTGACACAAGTGATAGATCTGTGAGTAAAAAGGTGCGAGAAGCTCAGGTGGCACAATACAACTACGTTCTAGTTGTTGGTGAGAAGGAAGCCAAAATTGACCAGGTGAGCTTACGGGCTAGAGATACAGAAGATGAAGATGTCACAGTAATGAGCATTGATGATCTTCTCCAATTCTTCAAAGACGAAGTTGAAGCTTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

735

Amino Acids

84.94

Weight (kDa)

6.55

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	123 - 181	2.1e-14	TGS domain
tRNA_SAD	PF07973	289 - 338	4.2e-13	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	438 - 626	2.1e-33	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	638 - 729	2.4e-18	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1179
AarI	CACCTGC	1 cut(s)	376
Acc36I	ACCTGC	1 cut(s)	376
AccI	GTMKAC	3 cut(s)	873, 1574, 1861
AccII	CGCG	1 cut(s)	993
AccIII	TCCGGA	1 cut(s)	1027
AciI	CCGC	2 cut(s)	180, 376
AclWI	GGATC	3 cut(s)	7, 1833, 1846
AcsI	RAATTY	3 cut(s)	243, 821, 1427
AcuI	CTGAAG	1 cut(s)	948
AfaI	GTAC	2 cut(s)	49, 105
AfiI	CCNNNNNNNGG	2 cut(s)	271, 975
AflII	CTTAAG	1 cut(s)	530
AgeI	ACCGGT	1 cut(s)	172
AjiI	CACGTC	1 cut(s)	1910
AjnI	CCWGG	3 cut(s)	258, 1360, 2103
AleI	CACNNNNGTG	1 cut(s)	582
Alw21I	GWGCWC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
Alw26I	GTCTC	3 cut(s)	291, 1598, 1936
Alw44I	GTGCAC	1 cut(s)	1461
AlwI	GGATC	3 cut(s)	7, 1833, 1846
Ama87I	CYCGRG	2 cut(s)	800, 1138
Aor13HI	TCCGGA	1 cut(s)	1027
ApaLI	GTGCAC	1 cut(s)	1461
ApeKI	GCWGC	3 cut(s)	1003, 1060, 1438
ApoI	RAATTY	3 cut(s)	243, 821, 1427
AsiGI	ACCGGT	1 cut(s)	172
Asp700I	GAANNNNTTC	2 cut(s)	1377, 1974
AspLEI	GCGC	2 cut(s)	774, 1985
AspS9I	GGNCC	8 cut(s)	142, 262, 274, 641, 796, 908, 1742, 1890
AsuC2I	CCSGG	3 cut(s)	295, 1139, 1140
AsuHPI	GGTGA	7 cut(s)	169, 400, 1118, 1147, 1246, 2093, 2119
AsuII	TTCGAA	2 cut(s)	443, 458
AvaI	CYCGRG	2 cut(s)	800, 1138
AvaII	GGWCC	4 cut(s)	142, 641, 908, 1742
BaeGI	GKGCMC	1 cut(s)	1465
BamHI	GGATCC	1 cut(s)	1838
BanII	GRGCYC	2 cut(s)	1116, 1171
BauI	CACGAG	1 cut(s)	1479
BbsI	GAAGAC	1 cut(s)	555
Bbv12I	GWGCWC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
BbvCI	CCTCAGC	2 cut(s)	432, 474
BbvI	GCAGC	3 cut(s)	1015, 1072, 1425
BccI	CCATC	7 cut(s)	263, 310, 374, 385, 1414, 1463, 1721
BceAI	ACGGC	1 cut(s)	546
BcgI	CGANNNNNNTGC	2 cut(s)	1934, 1968
BciT130I	CCWGG	3 cut(s)	260, 1362, 2105
BcnI	CCSGG	3 cut(s)	295, 1139, 1140
BcoDI	GTCTC	3 cut(s)	291, 1598, 1936
BfaI	CTAG	4 cut(s)	582, 1466, 2072, 2121
BfmI	CTRYAG	3 cut(s)	852, 1001, 1958
BfrI	CTTAAG	1 cut(s)	530
BfuAI	ACCTGC	1 cut(s)	376
BglI	GCCNNNNNGGC	1 cut(s)	1897
BglII	AGATCT	1 cut(s)	2018
BisI	GCNGC	3 cut(s)	1004, 1061, 1439
BlsI	GCNGC	3 cut(s)	1005, 1062, 1440
BmcAI	AGTACT	1 cut(s)	105
Bme1390I	CCNGG	6 cut(s)	260, 295, 1139, 1140, 1362, 2105
Bme18I	GGWCC	4 cut(s)	142, 641, 908, 1742
BmeT110I	CYCGRG	2 cut(s)	800, 1138
BmgBI	CACGTC	1 cut(s)	1910
BmgT120I	GGNCC	8 cut(s)	142, 262, 274, 641, 796, 908, 1742, 1890
BmiI	GGNNCC	2 cut(s)	643, 1840
BmrFI	CCNGG	6 cut(s)	260, 295, 1139, 1140, 1362, 2105
BmsI	GCATC	5 cut(s)	614, 667, 1179, 1450, 1495
BoxI	GACNNNNGTC	2 cut(s)	900, 1481
BpiI	GAAGAC	1 cut(s)	555
BplI	GAGNNNNNCTC	2 cut(s)	794, 826
BpmI	CTGGAG	1 cut(s)	994
Bpu10I	CCTNAGC	3 cut(s)	432, 474, 2046
Bpu14I	TTCGAA	2 cut(s)	443, 458
BpuEI	CTTGAG	2 cut(s)	629, 1532
BpuMI	CCSGG	3 cut(s)	295, 1139, 1140
BsaBI	GATNNNNATC	1 cut(s)	833
BsaI	GGTCTC	1 cut(s)	1598
BsaJI	CCNNGG	9 cut(s)	135, 183, 213, 293, 1138, 1636, 1665, 1706, 1944
BsaWI	WCCGGW	2 cut(s)	172, 1027
Bsc4I	CCNNNNNNNGG	2 cut(s)	271, 975
Bse118I	RCCGGY	2 cut(s)	144, 172
Bse1I	ACTGG	1 cut(s)	977
Bse3DI	GCAATG	1 cut(s)	371
Bse8I	GATNNNNATC	1 cut(s)	833
BseAI	TCCGGA	1 cut(s)	1027
BseBI	CCWGG	3 cut(s)	260, 1362, 2105
BseDI	CCNNGG	9 cut(s)	135, 183, 213, 293, 1138, 1636, 1665, 1706, 1944
BseGI	GGATG	7 cut(s)	255, 385, 629, 926, 1425, 1507, 1732
BseJI	GATNNNNATC	1 cut(s)	833
BseLI	CCNNNNNNNGG	2 cut(s)	271, 975
BseMI	GCAATG	1 cut(s)	371
BseMII	CTCAG	5 cut(s)	39, 423, 465, 1947, 2060
BseNI	ACTGG	1 cut(s)	977
BseRI	GAGGAG	1 cut(s)	449
BseSI	GKGCMC	1 cut(s)	1465
BseXI	GCAGC	3 cut(s)	1015, 1072, 1425
BseYI	CCCAGC	1 cut(s)	16
BsgI	GTGCAG	2 cut(s)	631, 1517
Bsh1236I	CGCG	1 cut(s)	993
BshTI	ACCGGT	1 cut(s)	172
BsiHKAI	GWGCWC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
BsiHKCI	CYCGRG	2 cut(s)	800, 1138
BsiSI	CCGG	5 cut(s)	145, 173, 295, 1028, 1139
BslFI	GGGAC	4 cut(s)	499, 509, 627, 1513
BslI	CCNNNNNNNGG	2 cut(s)	271, 975
BsmAI	GTCTC	3 cut(s)	291, 1598, 1936
BsmBI	CGTCTC	1 cut(s)	1936
BsmFI	GGGAC	4 cut(s)	499, 509, 627, 1513
Bso31I	GGTCTC	1 cut(s)	1598
BsoBI	CYCGRG	2 cut(s)	800, 1138
Bsp119I	TTCGAA	2 cut(s)	443, 458
Bsp1286I	GDGCHC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
Bsp13I	TCCGGA	1 cut(s)	1027
Bsp143I	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
Bsp19I	CCATGG	3 cut(s)	183, 213, 1636
Bsp68I	TCGCGA	1 cut(s)	993
BspACI	CCGC	2 cut(s)	180, 376
BspCNI	CTCAG	5 cut(s)	38, 424, 466, 1946, 2059
BspEI	TCCGGA	1 cut(s)	1027
BspFNI	CGCG	1 cut(s)	993
BspLI	GGNNCC	2 cut(s)	643, 1840
BspMAI	CTGCAG	1 cut(s)	1005
BspMI	ACCTGC	1 cut(s)	376
BspPI	GGATC	3 cut(s)	7, 1833, 1846
BspQI	GCTCTTC	1 cut(s)	67
BspT104I	TTCGAA	2 cut(s)	443, 458
BspTI	CTTAAG	1 cut(s)	530
BspTNI	GGTCTC	1 cut(s)	1598
BsrDI	GCAATG	1 cut(s)	371
BsrFI	RCCGGY	2 cut(s)	144, 172
BsrI	ACTGG	1 cut(s)	977
BssAI	RCCGGY	2 cut(s)	144, 172
BssECI	CCNNGG	9 cut(s)	135, 183, 213, 293, 1138, 1636, 1665, 1706, 1944
BssMI	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
BssNAI	GTATAC	3 cut(s)	874, 1575, 1862
BssSI	CACGAG	1 cut(s)	1479
BssT1I	CCWWGG	4 cut(s)	183, 213, 1636, 1706
Bst1107I	GTATAC	3 cut(s)	874, 1575, 1862
Bst2BI	CACGAG	1 cut(s)	1479
Bst2UI	CCWGG	3 cut(s)	260, 1362, 2105
Bst4CI	ACNGT	6 cut(s)	524, 762, 871, 1358, 1370, 2149
Bst6I	CTCTTC	6 cut(s)	67, 294, 440, 654, 1368, 1419
BstAFI	CTTAAG	1 cut(s)	530
BstBI	TTCGAA	2 cut(s)	443, 458
BstC8I	GCNNGC	4 cut(s)	629, 1047, 1284, 1703
BstDEI	CTNAG	6 cut(s)	25, 432, 474, 1901, 1933, 2046
BstDSI	CCRYGG	4 cut(s)	135, 183, 213, 1636
BstEII	GGTNACC	1 cut(s)	175
BstENI	CCTNNNNNAGG	1 cut(s)	973
BstF5I	GGATG	7 cut(s)	255, 385, 629, 926, 1425, 1507, 1732
BstFNI	CGCG	1 cut(s)	993
BstHHI	GCGC	2 cut(s)	774, 1985
BstKTI	GATC	8 cut(s)	15, 94, 115, 837, 991, 1841, 2021, 2167
BstMAI	GTCTC	3 cut(s)	291, 1598, 1936
BstMBI	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
BstNI	CCWGG	3 cut(s)	260, 1362, 2105
BstNSI	RCATGY	1 cut(s)	1286
BstPAI	GACNNNNGTC	2 cut(s)	900, 1481
BstPI	GGTNACC	1 cut(s)	175
BstSCI	CCNGG	6 cut(s)	258, 293, 1137, 1138, 1360, 2103
BstSFI	CTRYAG	3 cut(s)	852, 1001, 1958
BstSLI	GKGCMC	1 cut(s)	1465
BstUI	CGCG	1 cut(s)	993
BstV1I	GCAGC	3 cut(s)	1015, 1072, 1425
BstV2I	GAAGAC	1 cut(s)	555
BstX2I	RGATCY	2 cut(s)	1838, 2018
BstYI	RGATCY	2 cut(s)	1838, 2018
BstZ17I	GTATAC	3 cut(s)	874, 1575, 1862
BtgI	CCRYGG	4 cut(s)	135, 183, 213, 1636
BtrI	CACGTC	1 cut(s)	1910
BtsCI	GGATG	7 cut(s)	255, 385, 629, 926, 1425, 1507, 1732
BtsIMutI	CAGTG	4 cut(s)	487, 767, 1366, 1462
BtuMI	TCGCGA	1 cut(s)	993
BveI	ACCTGC	1 cut(s)	376
Cac8I	GCNNGC	4 cut(s)	629, 1047, 1284, 1703
CfoI	GCGC	2 cut(s)	774, 1985
Cfr10I	RCCGGY	2 cut(s)	144, 172
Cfr13I	GGNCC	8 cut(s)	142, 262, 274, 641, 796, 908, 1742, 1890
Cfr9I	CCCGGG	1 cut(s)	1138
CsiI	ACCWGGT	1 cut(s)	2103
Csp6I	GTAC	2 cut(s)	48, 104
CspAI	ACCGGT	1 cut(s)	172
CviQI	GTAC	2 cut(s)	48, 104
DdeI	CTNAG	6 cut(s)	25, 432, 474, 1901, 1933, 2046
DpnI	GATC	8 cut(s)	14, 93, 114, 836, 990, 1840, 2020, 2166
DpnII	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
Eam1104I	CTCTTC	6 cut(s)	67, 294, 440, 654, 1368, 1419
EarI	CTCTTC	6 cut(s)	67, 294, 440, 654, 1368, 1419
Ecl136II	GAGCTC	2 cut(s)	1114, 1169
Eco130I	CCWWGG	4 cut(s)	183, 213, 1636, 1706
Eco147I	AGGCCT	3 cut(s)	508, 1390, 1810
Eco24I	GRGCYC	2 cut(s)	1116, 1171
Eco31I	GGTCTC	1 cut(s)	1598
Eco32I	GATATC	1 cut(s)	1226
Eco47I	GGWCC	4 cut(s)	142, 641, 908, 1742
Eco53kI	GAGCTC	2 cut(s)	1114, 1169
Eco57I	CTGAAG	1 cut(s)	948
Eco88I	CYCGRG	2 cut(s)	800, 1138
Eco91I	GGTNACC	1 cut(s)	175
EcoICRI	GAGCTC	2 cut(s)	1114, 1169
EcoNI	CCTNNNNNAGG	1 cut(s)	973
EcoO109I	RGGNCCY	1 cut(s)	796
EcoO65I	GGTNACC	1 cut(s)	175
EcoRII	CCWGG	3 cut(s)	258, 1360, 2103
EcoRV	GATATC	1 cut(s)	1226
EcoT14I	CCWWGG	4 cut(s)	183, 213, 1636, 1706
EcoT22I	ATGCAT	1 cut(s)	682
EcoT38I	GRGCYC	2 cut(s)	1116, 1171
ErhI	CCWWGG	4 cut(s)	183, 213, 1636, 1706
Esp3I	CGTCTC	1 cut(s)	1936
FaqI	GGGAC	4 cut(s)	499, 509, 627, 1513
FauI	CCCGC	1 cut(s)	369
FblI	GTMKAC	3 cut(s)	873, 1574, 1861
Fnu4HI	GCNGC	3 cut(s)	1004, 1061, 1439
FokI	GGATG	7 cut(s)	242, 392, 636, 913, 1432, 1514, 1739
FriOI	GRGCYC	2 cut(s)	1116, 1171
Fsp4HI	GCNGC	3 cut(s)	1004, 1061, 1439
FspBI	CTAG	4 cut(s)	582, 1466, 2072, 2121
GlaI	GCGC	2 cut(s)	773, 1984
GluI	GCNGC	3 cut(s)	1004, 1061, 1439
GsaI	CCCAGC	1 cut(s)	20
GsuI	CTGGAG	1 cut(s)	994
HapII	CCGG	5 cut(s)	145, 173, 295, 1028, 1139
HhaI	GCGC	2 cut(s)	774, 1985
Hin6I	GCGC	2 cut(s)	772, 1983
HinP1I	GCGC	2 cut(s)	772, 1983
HincII	GTYRAC	1 cut(s)	2005
HindII	GTYRAC	1 cut(s)	2005
HindIII	AAGCTT	2 cut(s)	462, 2196
HpaII	CCGG	5 cut(s)	145, 173, 295, 1028, 1139
HphI	GGTGA	7 cut(s)	169, 400, 1118, 1147, 1246, 2093, 2119
Hpy166II	GTNNAC	8 cut(s)	142, 874, 895, 1463, 1575, 1862, 1907, 2005
Hpy188III	TCNNGA	7 cut(s)	802, 826, 992, 1028, 1229, 1486, 1967
Hpy8I	GTNNAC	8 cut(s)	142, 874, 895, 1463, 1575, 1862, 1907, 2005
HpyAV	CCTTC	7 cut(s)	580, 736, 949, 1715, 1787, 1903, 2080
HpyCH4III	ACNGT	6 cut(s)	524, 762, 871, 1358, 1370, 2149
HpyCH4IV	ACGT	3 cut(s)	1909, 1995, 2067
HpyF3I	CTNAG	6 cut(s)	25, 432, 474, 1901, 1933, 2046
HpySE526I	ACGT	3 cut(s)	1909, 1995, 2067
HspAI	GCGC	2 cut(s)	772, 1983
Kpn2I	TCCGGA	1 cut(s)	1027
Kzo9I	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
LguI	GCTCTTC	1 cut(s)	67
LmnI	GCTCC	5 cut(s)	514, 1111, 1166, 1609, 1870
Lsp1109I	GCAGC	3 cut(s)	1015, 1072, 1425
LweI	GCATC	5 cut(s)	614, 667, 1179, 1450, 1495
MabI	ACCWGGT	1 cut(s)	2103
MaeI	CTAG	4 cut(s)	582, 1466, 2072, 2121
MaeII	ACGT	3 cut(s)	1909, 1995, 2067
MaeIII	GTNAC	8 cut(s)	127, 175, 518, 563, 1124, 1364, 1631, 2143
MalI	GATC	8 cut(s)	14, 93, 114, 836, 990, 1840, 2020, 2166
MboI	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
MfeI	CAATTG	1 cut(s)	1917
MflI	RGATCY	2 cut(s)	1838, 2018
MhlI	GDGCHC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
MlyI	GAGTC	5 cut(s)	227, 260, 481, 1471, 1535
Mph1103I	ATGCAT	1 cut(s)	682
MroI	TCCGGA	1 cut(s)	1027
MroXI	GAANNNNTTC	2 cut(s)	1377, 1974
MseI	TTAA	2 cut(s)	531, 716
MslI	CAYNNNNRTG	6 cut(s)	182, 582, 946, 1446, 1998, 2150
MspCI	CTTAAG	1 cut(s)	530
MspI	CCGG	5 cut(s)	145, 173, 295, 1028, 1139
MspR9I	CCNGG	6 cut(s)	260, 295, 1139, 1140, 1362, 2105
MunI	CAATTG	1 cut(s)	1917
MvaI	CCWGG	3 cut(s)	260, 1362, 2105
MvnI	CGCG	1 cut(s)	993
NciI	CCSGG	3 cut(s)	295, 1139, 1140
NcoI	CCATGG	3 cut(s)	183, 213, 1636
NdeII	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
NlaIV	GGNNCC	2 cut(s)	643, 1840
NmeAIII	GCCGAG	1 cut(s)	1690
NmuCI	GTSAC	6 cut(s)	175, 518, 563, 1124, 1364, 2143
NruI	TCGCGA	1 cut(s)	993
NsiI	ATGCAT	1 cut(s)	682
NspI	RCATGY	1 cut(s)	1286
NspV	TTCGAA	2 cut(s)	443, 458
OliI	CACNNNNGTG	1 cut(s)	582
PaeI	GCATGC	1 cut(s)	1286
PaeR7I	CTCGAG	1 cut(s)	800
PaqCI	CACCTGC	1 cut(s)	376
PceI	AGGCCT	3 cut(s)	508, 1390, 1810
PciSI	GCTCTTC	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	1377, 1974
PfeI	GAWTC	6 cut(s)	96, 237, 298, 728, 1491, 1975
PinAI	ACCGGT	1 cut(s)	172
PkrI	GCNGC	3 cut(s)	1005, 1062, 1440
PleI	GAGTC	5 cut(s)	226, 259, 481, 1471, 1534
PpsI	GAGTC	5 cut(s)	226, 259, 481, 1471, 1534
PshAI	GACNNNNGTC	2 cut(s)	900, 1481
PsiI	TTATAA	1 cut(s)	1179
Psp124BI	GAGCTC	2 cut(s)	1116, 1171
Psp6I	CCWGG	3 cut(s)	258, 1360, 2103
PspEI	GGTNACC	1 cut(s)	175
PspFI	CCCAGC	1 cut(s)	16
PspGI	CCWGG	3 cut(s)	258, 1360, 2103
PspN4I	GGNNCC	2 cut(s)	643, 1840
PspPI	GGNCC	8 cut(s)	142, 262, 274, 641, 796, 908, 1742, 1890
PstI	CTGCAG	1 cut(s)	1005
PsuI	RGATCY	2 cut(s)	1838, 2018
RruI	TCGCGA	1 cut(s)	993
RsaI	GTAC	2 cut(s)	49, 105
RsaNI	GTAC	2 cut(s)	48, 104
RseI	CAYNNNNRTG	6 cut(s)	182, 582, 946, 1446, 1998, 2150
SacI	GAGCTC	2 cut(s)	1116, 1171
SapI	GCTCTTC	1 cut(s)	67
SaqAI	TTAA	2 cut(s)	531, 716
SatI	GCNGC	3 cut(s)	1004, 1061, 1439
Sau3AI	GATC	8 cut(s)	12, 91, 112, 834, 988, 1838, 2018, 2164
Sau96I	GGNCC	8 cut(s)	142, 262, 274, 641, 796, 908, 1742, 1890
ScaI	AGTACT	1 cut(s)	105
SchI	GAGTC	5 cut(s)	227, 260, 481, 1471, 1535
ScrFI	CCNGG	6 cut(s)	260, 295, 1139, 1140, 1362, 2105
SduI	GDGCHC	6 cut(s)	589, 714, 1116, 1171, 1465, 1875
SexAI	ACCWGGT	1 cut(s)	2103
SfaNI	GCATC	5 cut(s)	614, 667, 1179, 1450, 1495
SfcI	CTRYAG	3 cut(s)	852, 1001, 1958
Sfr274I	CTCGAG	1 cut(s)	800
SfuI	TTCGAA	2 cut(s)	443, 458
SinI	GGWCC	4 cut(s)	142, 641, 908, 1742
SlaI	CTCGAG	1 cut(s)	800
SmaI	CCCGGG	1 cut(s)	1140
SmiMI	CAYNNNNRTG	6 cut(s)	182, 582, 946, 1446, 1998, 2150
SmlI	CTYRAG	4 cut(s)	530, 608, 800, 1547
SmoI	CTYRAG	4 cut(s)	530, 608, 800, 1547
SphI	GCATGC	1 cut(s)	1286
SseBI	AGGCCT	3 cut(s)	508, 1390, 1810
SsiI	CCGC	2 cut(s)	180, 376
SspI	AATATT	1 cut(s)	1211
SspMI	CTAG	4 cut(s)	582, 1466, 2072, 2121
SstI	GAGCTC	2 cut(s)	1116, 1171
StuI	AGGCCT	3 cut(s)	508, 1390, 1810
StyD4I	CCNGG	6 cut(s)	258, 293, 1137, 1138, 1360, 2103
StyI	CCWWGG	4 cut(s)	183, 213, 1636, 1706
TaaI	ACNGT	6 cut(s)	524, 762, 871, 1358, 1370, 2149
TaiI	ACGT	3 cut(s)	1912, 1998, 2070
TatI	WGTACW	1 cut(s)	103
TfiI	GAWTC	6 cut(s)	96, 237, 298, 728, 1491, 1975
Tru1I	TTAA	2 cut(s)	531, 716
Tru9I	TTAA	2 cut(s)	531, 716
TscAI	CASTG	4 cut(s)	487, 767, 1373, 1462
TseFI	GTSAC	6 cut(s)	175, 518, 563, 1124, 1364, 2143
TseI	GCWGC	3 cut(s)	1003, 1060, 1438
Tsp45I	GTSAC	6 cut(s)	175, 518, 563, 1124, 1364, 2143
TspGWI	ACGGA	3 cut(s)	124, 1179, 1987
TspMI	CCCGGG	1 cut(s)	1138
TspRI	CASTG	4 cut(s)	487, 767, 1373, 1462
Vha464I	CTTAAG	1 cut(s)	530
VneI	GTGCAC	1 cut(s)	1461
VpaK11BI	GGWCC	4 cut(s)	142, 641, 908, 1742
XagI	CCTNNNNNAGG	1 cut(s)	973
XapI	RAATTY	3 cut(s)	243, 821, 1427
XceI	RCATGY	1 cut(s)	1286
XhoI	CTCGAG	1 cut(s)	800
XmaI	CCCGGG	1 cut(s)	1138
XmiI	GTMKAC	3 cut(s)	873, 1574, 1861
XmnI	GAANNNNTTC	2 cut(s)	1377, 1974
XspI	CTAG	4 cut(s)	582, 1466, 2072, 2121
ZrmI	AGTACT	1 cut(s)	105
Zsp2I	ATGCAT	1 cut(s)	682

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.