Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

14623777 .. 14627515

Gene Structure 3739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG141100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGGAGGGGAAGAAGTGGATAACTTCCCCTTTGGAAATTGCTGAGGAGATTTCGAAGAGTTTGGCTTCGAAAGCTTTGATTGCTGAGGTCAGTGGGACTCTATGGGACATGGCAAGGCCTTTGGAGCGTGACTGTGAGCTTAAGATATTCACATTTGAAGACAACAAAGGCCGTGACACCTTCTGGCACTCTAGTGCTCATATTCTTGGACAGGCACTTGAGGCCAAGTATGGATGCAAGCTCTGTATTGGTCCCTGCACAACAAAAGAAGAGGGGTTCTACTATGATGCATACTATGGCAGTTTGGGCTTGAATGATGAGCACTTAAAGAAAATTGAATCAGAGGTAGAGAAGGCTGTTAAGGCCAAACAGTGCTTTGAGCGCATTGAAGTATCAATGGAACAGGCCCTCGAGATTTTCTCTGATAACAAATTCAAGATTGAGATCATCAATGAGCTTCCTATAGATAAGGCTATAACTGTATACAGATGTGGCAACTTGGTGGACTTGTGCCGTGGACCTCATATCCCAAATACATCCTTTGTCAAAGCATTTAGATGTTTGAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGCGAAAGTCTGCAGCGAGTTTATGGAATATCATATCCGGATAAAAAACTTTTGCAGGCATATATCAAGCAGCAGGAAGAAGCAAAAAAATATGACCATAGGTTGTTGGGTAAATCACAGGAGCTCATATTTTGTCACCCATATAGCCCAGGAAGTTGGTTCTTCACCCCCAACGGAGCTCGGATTTATAAGAAATTAATGCAGTTCATGGAAAATGAATATTTGGAAAGAGGATATCAGGAGGTTATAACACCAAACATATTCAAAATGGATTTGTTTGAAAAATCTAAGCATGCAGGACACTACAAACAGAATATGTTTCTTTTAGAGATTGAGAAAGAGGAATATGGTTTGAAGCCAATGAACTGTCCTGGTCACTGTGAAGAGTTTAGACATAGGCCTCGTTCTTATAGAGAACTTCCTCTTCGGATGGCTGAATTTGGGGTGCTGCATCGAAATGAGGCCAGTGGTGCACTAGATGGATTGACTCGTGTCAGGAGATTCGTGCAGGATGATGCTCATATATTTTGTAGGGAGTCCCAACTGAAAGATGAACTCAAGGGTGTCCTCGATTTCATCAAGTATACCTATGACATCTTTGGTCTCTCTTTTGATGTGGAGCTATCAACAAGGCCAGAAAGTTACCATGGAGAACGAGCAACATGGGATAAAGCCGAGGCAATTCTTACAGAAGCACTAAATGAGTTTGGCAAGCCTTGGCAGATAAATGAAGGGGATGGGGCATTTTATGGACCAAAGATAGATTTCGGTGTGTGTAATGCTTTGAAGAAAAAATTTCAGTGTGCAACAATACAGCTTGATTTTCAGCTCCCTGATTATGAGCAGTTCGACCTACATTACAATTCAGAGGGTAAAGAAGGCAAGAGGGAGAGGCCTGTTATGATACACAGAGCTGTATTCGGCTCCTTTGAGCGTTTCTTTGGTATACTTTTGGAGCACTACAAAGGAAAATGGCCCTTCTGGCTTAGTCCACGTCAGGCAATTGTTTGCCCCATCTCAGAGAAATCCGAGGCTTATGCTCTACAGGTTCAGGAACGGATTCGTCGCGCTGGTTATCATGTTGATGTTGACACAAGTGATAGATCCGTGAGTAAAAAGGTGCGAGAAGCTCAGGTGGCACAATACAACTACGTTCTAGTTGTTGGTGAGAAGGAAGCCAAAATTGACCAGGTGAGCTTACGGGCTAGAGATACAGAAGATGAAGATGTCACAGTAATGAGCATTGATGATCTTCTCCAATTCTTCAAAGACGAAGTTGAAGCTTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

630

Amino Acids

73.19

Weight (kDa)

6.06

Isoelectric Point (pI)

39.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	2 - 51	7.5e-11	TGS domain
tRNA_SAD	PF07973	159 - 208	3.4e-14	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	308 - 521	2.6e-43	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	533 - 624	1.4e-18	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	789, 848
AccI	GTMKAC	3 cut(s)	483, 1184, 1546
AccII	CGCG	2 cut(s)	603, 1668
AccIII	TCCGGA	1 cut(s)	637
AclWI	GGATC	1 cut(s)	1698
AcsI	RAATTY	3 cut(s)	431, 1037, 1394
AcuI	CTGAAG	1 cut(s)	558
AfiI	CCNNNNNNNGG	1 cut(s)	585
AflII	CTTAAG	1 cut(s)	140
AjiI	CACGTC	1 cut(s)	1595
AjnI	CCWGG	3 cut(s)	748, 970, 1788
AleI	CACNNNNGTG	1 cut(s)	192
Alw21I	GWGCWC	6 cut(s)	199, 324, 726, 781, 1075, 1560
Alw26I	GTCTC	1 cut(s)	1208
Alw44I	GTGCAC	1 cut(s)	1071
AlwI	GGATC	1 cut(s)	1698
Ama87I	CYCGRG	1 cut(s)	410
Aor13HI	TCCGGA	1 cut(s)	637
ApaLI	GTGCAC	1 cut(s)	1071
ApeKI	GCWGC	3 cut(s)	613, 670, 1048
ApoI	RAATTY	3 cut(s)	431, 1037, 1394
AseI	ATTAAT	1 cut(s)	797
Asp700I	GAANNNNTTC	1 cut(s)	1659
AspLEI	GCGC	2 cut(s)	384, 1670
AspS9I	GGNCC	5 cut(s)	251, 406, 518, 1352, 1575
AsuHPI	GGTGA	4 cut(s)	728, 757, 1778, 1804
AsuII	TTCGAA	2 cut(s)	53, 68
AvaI	CYCGRG	1 cut(s)	410
AvaII	GGWCC	3 cut(s)	251, 518, 1352
BaeGI	GKGCMC	1 cut(s)	1075
BanII	GRGCYC	2 cut(s)	726, 781
BauI	CACGAG	1 cut(s)	1089
BbsI	GAAGAC	1 cut(s)	165
Bbv12I	GWGCWC	6 cut(s)	199, 324, 726, 781, 1075, 1560
BbvCI	CCTCAGC	2 cut(s)	42, 84
BbvI	GCAGC	3 cut(s)	625, 682, 1035
BccI	CCATC	4 cut(s)	1024, 1073, 1331, 1622
BceAI	ACGGC	2 cut(s)	156, 498
BcgI	CGANNNNNNTGC	2 cut(s)	1619, 1653
BciT130I	CCWGG	3 cut(s)	750, 972, 1790
BcoDI	GTCTC	1 cut(s)	1208
BfaI	CTAG	4 cut(s)	192, 1076, 1757, 1806
BfmI	CTRYAG	3 cut(s)	462, 611, 1643
BfrI	CTTAAG	1 cut(s)	140
BglI	GCCNNNNNGGC	1 cut(s)	1582
BisI	GCNGC	3 cut(s)	614, 671, 1049
BlsI	GCNGC	3 cut(s)	615, 672, 1050
Bme1390I	CCNGG	3 cut(s)	750, 972, 1790
Bme18I	GGWCC	3 cut(s)	251, 518, 1352
BmeT110I	CYCGRG	1 cut(s)	410
BmgBI	CACGTC	1 cut(s)	1595
BmgT120I	GGNCC	5 cut(s)	251, 406, 518, 1352, 1575
BmiI	GGNNCC	2 cut(s)	253, 1525
BmrFI	CCNGG	3 cut(s)	750, 972, 1790
BmsI	GCATC	4 cut(s)	224, 277, 1060, 1105
BoxI	GACNNNNGTC	1 cut(s)	1091
BpiI	GAAGAC	1 cut(s)	165
BplI	GAGNNNNNCTC	2 cut(s)	404, 436
BpmI	CTGGAG	1 cut(s)	604
Bpu10I	CCTNAGC	3 cut(s)	42, 84, 1731
Bpu14I	TTCGAA	2 cut(s)	53, 68
BpuEI	CTTGAG	2 cut(s)	239, 1142
BsaBI	GATNNNNATC	1 cut(s)	443
BsaI	GGTCTC	1 cut(s)	1208
BsaJI	CCNNGG	6 cut(s)	514, 748, 1246, 1275, 1316, 1629
BsaWI	WCCGGW	1 cut(s)	637
Bsc4I	CCNNNNNNNGG	1 cut(s)	585
Bse1I	ACTGG	2 cut(s)	587, 1065
Bse8I	GATNNNNATC	1 cut(s)	443
BseAI	TCCGGA	1 cut(s)	637
BseBI	CCWGG	3 cut(s)	750, 972, 1790
BseDI	CCNNGG	6 cut(s)	514, 748, 1246, 1275, 1316, 1629
BseGI	GGATG	5 cut(s)	239, 536, 1035, 1117, 1342
BseJI	GATNNNNATC	1 cut(s)	443
BseLI	CCNNNNNNNGG	1 cut(s)	585
BseMII	CTCAG	4 cut(s)	33, 75, 1632, 1745
BseNI	ACTGG	2 cut(s)	587, 1065
BseRI	GAGGAG	1 cut(s)	59
BseSI	GKGCMC	1 cut(s)	1075
BseXI	GCAGC	3 cut(s)	625, 682, 1035
BsgI	GTGCAG	2 cut(s)	241, 1127
Bsh1236I	CGCG	2 cut(s)	603, 1668
BsiHKAI	GWGCWC	6 cut(s)	199, 324, 726, 781, 1075, 1560
BsiHKCI	CYCGRG	1 cut(s)	410
BsiSI	CCGG	1 cut(s)	638
BslFI	GGGAC	4 cut(s)	109, 119, 237, 1123
BslI	CCNNNNNNNGG	1 cut(s)	585
BsmAI	GTCTC	1 cut(s)	1208
BsmFI	GGGAC	4 cut(s)	109, 119, 237, 1123
Bso31I	GGTCTC	1 cut(s)	1208
BsoBI	CYCGRG	1 cut(s)	410
Bsp119I	TTCGAA	2 cut(s)	53, 68
Bsp1286I	GDGCHC	6 cut(s)	199, 324, 726, 781, 1075, 1560
Bsp13I	TCCGGA	1 cut(s)	637
Bsp143I	GATC	4 cut(s)	444, 598, 1703, 1849
Bsp19I	CCATGG	1 cut(s)	1246
Bsp68I	TCGCGA	1 cut(s)	603
BspCNI	CTCAG	4 cut(s)	34, 76, 1631, 1744
BspEI	TCCGGA	1 cut(s)	637
BspFNI	CGCG	2 cut(s)	603, 1668
BspLI	GGNNCC	2 cut(s)	253, 1525
BspMAI	CTGCAG	1 cut(s)	615
BspPI	GGATC	1 cut(s)	1698
BspT104I	TTCGAA	2 cut(s)	53, 68
BspTI	CTTAAG	1 cut(s)	140
BspTNI	GGTCTC	1 cut(s)	1208
BsrI	ACTGG	2 cut(s)	587, 1065
BssECI	CCNNGG	6 cut(s)	514, 748, 1246, 1275, 1316, 1629
BssMI	GATC	4 cut(s)	444, 598, 1703, 1849
BssNAI	GTATAC	3 cut(s)	484, 1185, 1547
BssSI	CACGAG	1 cut(s)	1089
BssT1I	CCWWGG	2 cut(s)	1246, 1316
Bst1107I	GTATAC	3 cut(s)	484, 1185, 1547
Bst2BI	CACGAG	1 cut(s)	1089
Bst2UI	CCWGG	3 cut(s)	750, 972, 1790
Bst4CI	ACNGT	6 cut(s)	134, 372, 481, 968, 980, 1834
Bst6I	CTCTTC	4 cut(s)	50, 264, 978, 1029
BstAFI	CTTAAG	1 cut(s)	140
BstBI	TTCGAA	2 cut(s)	53, 68
BstC8I	GCNNGC	4 cut(s)	239, 657, 894, 1313
BstDEI	CTNAG	6 cut(s)	42, 84, 888, 1586, 1618, 1731
BstDSI	CCRYGG	2 cut(s)	514, 1246
BstENI	CCTNNNNNAGG	1 cut(s)	583
BstF5I	GGATG	5 cut(s)	239, 536, 1035, 1117, 1342
BstFNI	CGCG	2 cut(s)	603, 1668
BstHHI	GCGC	2 cut(s)	384, 1670
BstKTI	GATC	4 cut(s)	447, 601, 1706, 1852
BstMAI	GTCTC	1 cut(s)	1208
BstMBI	GATC	4 cut(s)	444, 598, 1703, 1849
BstNI	CCWGG	3 cut(s)	750, 972, 1790
BstNSI	RCATGY	1 cut(s)	896
BstPAI	GACNNNNGTC	1 cut(s)	1091
BstSCI	CCNGG	3 cut(s)	748, 970, 1788
BstSFI	CTRYAG	3 cut(s)	462, 611, 1643
BstSLI	GKGCMC	1 cut(s)	1075
BstUI	CGCG	2 cut(s)	603, 1668
BstV1I	GCAGC	3 cut(s)	625, 682, 1035
BstV2I	GAAGAC	1 cut(s)	165
BstX2I	RGATCY	1 cut(s)	1703
BstXI	CCANNNNNNTGG	1 cut(s)	756
BstYI	RGATCY	1 cut(s)	1703
BstZ17I	GTATAC	3 cut(s)	484, 1185, 1547
BtgI	CCRYGG	2 cut(s)	514, 1246
BtrI	CACGTC	1 cut(s)	1595
BtsCI	GGATG	5 cut(s)	239, 536, 1035, 1117, 1342
BtsIMutI	CAGTG	5 cut(s)	97, 377, 976, 1072, 1406
BtuMI	TCGCGA	1 cut(s)	603
Cac8I	GCNNGC	4 cut(s)	239, 657, 894, 1313
CfoI	GCGC	2 cut(s)	384, 1670
Cfr13I	GGNCC	5 cut(s)	251, 406, 518, 1352, 1575
CsiI	ACCWGGT	1 cut(s)	1788
CviAII	CATG	6 cut(s)	109, 808, 893, 1247, 1263, 1679
DdeI	CTNAG	6 cut(s)	42, 84, 888, 1586, 1618, 1731
DpnI	GATC	4 cut(s)	446, 600, 1705, 1851
DpnII	GATC	4 cut(s)	444, 598, 1703, 1849
Eam1104I	CTCTTC	4 cut(s)	50, 264, 978, 1029
EarI	CTCTTC	4 cut(s)	50, 264, 978, 1029
Ecl136II	GAGCTC	2 cut(s)	724, 779
Eco130I	CCWWGG	2 cut(s)	1246, 1316
Eco147I	AGGCCT	3 cut(s)	118, 1000, 1495
Eco24I	GRGCYC	2 cut(s)	726, 781
Eco31I	GGTCTC	1 cut(s)	1208
Eco32I	GATATC	1 cut(s)	836
Eco47I	GGWCC	3 cut(s)	251, 518, 1352
Eco53kI	GAGCTC	2 cut(s)	724, 779
Eco57I	CTGAAG	1 cut(s)	558
Eco88I	CYCGRG	1 cut(s)	410
EcoICRI	GAGCTC	2 cut(s)	724, 779
EcoNI	CCTNNNNNAGG	1 cut(s)	583
EcoO109I	RGGNCCY	1 cut(s)	406
EcoRII	CCWGG	3 cut(s)	748, 970, 1788
EcoRV	GATATC	1 cut(s)	836
EcoT14I	CCWWGG	2 cut(s)	1246, 1316
EcoT22I	ATGCAT	1 cut(s)	292
EcoT38I	GRGCYC	2 cut(s)	726, 781
ErhI	CCWWGG	2 cut(s)	1246, 1316
FaeI	CATG	6 cut(s)	112, 811, 896, 1250, 1266, 1682
FaqI	GGGAC	4 cut(s)	109, 119, 237, 1123
FatI	CATG	6 cut(s)	108, 807, 892, 1246, 1262, 1678
FblI	GTMKAC	3 cut(s)	483, 1184, 1546
Fnu4HI	GCNGC	3 cut(s)	614, 671, 1049
FokI	GGATG	5 cut(s)	246, 523, 1042, 1124, 1349
FriOI	GRGCYC	2 cut(s)	726, 781
Fsp4HI	GCNGC	3 cut(s)	614, 671, 1049
FspBI	CTAG	4 cut(s)	192, 1076, 1757, 1806
GlaI	GCGC	2 cut(s)	383, 1669
GluI	GCNGC	3 cut(s)	614, 671, 1049
GsuI	CTGGAG	1 cut(s)	604
HapII	CCGG	1 cut(s)	638
HhaI	GCGC	2 cut(s)	384, 1670
Hin1II	CATG	6 cut(s)	112, 811, 896, 1250, 1266, 1682
Hin6I	GCGC	2 cut(s)	382, 1668
HinP1I	GCGC	2 cut(s)	382, 1668
HincII	GTYRAC	1 cut(s)	1690
HindII	GTYRAC	1 cut(s)	1690
HindIII	AAGCTT	2 cut(s)	72, 1881
HinfI	GANTC	6 cut(s)	97, 338, 1087, 1101, 1136, 1660
HpaII	CCGG	1 cut(s)	638
HphI	GGTGA	4 cut(s)	728, 757, 1778, 1804
Hpy166II	GTNNAC	8 cut(s)	484, 505, 518, 1073, 1185, 1547, 1592, 1690
Hpy188I	TCNGA	7 cut(s)	343, 424, 783, 1029, 1468, 1621, 1630
Hpy188III	TCNNGA	7 cut(s)	412, 436, 602, 638, 839, 1096, 1652
Hpy8I	GTNNAC	8 cut(s)	484, 505, 518, 1073, 1185, 1547, 1592, 1690
Hpy99I	CGWCG	1 cut(s)	1668
HpyAV	CCTTC	7 cut(s)	190, 346, 559, 1325, 1472, 1588, 1765
HpyCH4III	ACNGT	6 cut(s)	134, 372, 481, 968, 980, 1834
HpyCH4IV	ACGT	2 cut(s)	1594, 1752
HpyF3I	CTNAG	6 cut(s)	42, 84, 888, 1586, 1618, 1731
HpySE526I	ACGT	2 cut(s)	1594, 1752
Hsp92II	CATG	6 cut(s)	112, 811, 896, 1250, 1266, 1682
HspAI	GCGC	2 cut(s)	382, 1668
Kpn2I	TCCGGA	1 cut(s)	637
Kzo9I	GATC	4 cut(s)	444, 598, 1703, 1849
LmnI	GCTCC	7 cut(s)	124, 721, 776, 1219, 1434, 1529, 1555
Lsp1109I	GCAGC	3 cut(s)	625, 682, 1035
LweI	GCATC	4 cut(s)	224, 277, 1060, 1105
MabI	ACCWGGT	1 cut(s)	1788
MaeI	CTAG	4 cut(s)	192, 1076, 1757, 1806
MaeII	ACGT	2 cut(s)	1594, 1752
MaeIII	GTNAC	6 cut(s)	128, 173, 734, 974, 1241, 1828
MalI	GATC	4 cut(s)	446, 600, 1705, 1851
MboI	GATC	4 cut(s)	444, 598, 1703, 1849
MfeI	CAATTG	1 cut(s)	1602
MflI	RGATCY	1 cut(s)	1703
MhlI	GDGCHC	6 cut(s)	199, 324, 726, 781, 1075, 1560
MlyI	GAGTC	3 cut(s)	91, 1081, 1145
Mph1103I	ATGCAT	1 cut(s)	292
MroI	TCCGGA	1 cut(s)	637
MroXI	GAANNNNTTC	1 cut(s)	1659
MseI	TTAA	4 cut(s)	141, 326, 360, 797
MslI	CAYNNNNRTG	5 cut(s)	192, 556, 1056, 1683, 1835
MspCI	CTTAAG	1 cut(s)	140
MspI	CCGG	1 cut(s)	638
MspR9I	CCNGG	3 cut(s)	750, 972, 1790
MunI	CAATTG	1 cut(s)	1602
MvaI	CCWGG	3 cut(s)	750, 972, 1790
MvnI	CGCG	2 cut(s)	603, 1668
NcoI	CCATGG	1 cut(s)	1246
NdeII	GATC	4 cut(s)	444, 598, 1703, 1849
NlaIII	CATG	6 cut(s)	112, 811, 896, 1250, 1266, 1682
NlaIV	GGNNCC	2 cut(s)	253, 1525
NmeAIII	GCCGAG	1 cut(s)	1300
NmuCI	GTSAC	5 cut(s)	128, 173, 734, 974, 1828
NruI	TCGCGA	1 cut(s)	603
NsiI	ATGCAT	1 cut(s)	292
NspI	RCATGY	1 cut(s)	896
NspV	TTCGAA	2 cut(s)	53, 68
OliI	CACNNNNGTG	1 cut(s)	192
PaeI	GCATGC	1 cut(s)	896
PaeR7I	CTCGAG	1 cut(s)	410
PceI	AGGCCT	3 cut(s)	118, 1000, 1495
PdmI	GAANNNNTTC	1 cut(s)	1659
PfeI	GAWTC	3 cut(s)	338, 1101, 1660
PkrI	GCNGC	3 cut(s)	615, 672, 1050
PleI	GAGTC	3 cut(s)	91, 1081, 1144
PpsI	GAGTC	3 cut(s)	91, 1081, 1144
PshAI	GACNNNNGTC	1 cut(s)	1091
PshBI	ATTAAT	1 cut(s)	797
PsiI	TTATAA	2 cut(s)	789, 848
Psp124BI	GAGCTC	2 cut(s)	726, 781
Psp6I	CCWGG	3 cut(s)	748, 970, 1788
PspGI	CCWGG	3 cut(s)	748, 970, 1788
PspN4I	GGNNCC	2 cut(s)	253, 1525
PspPI	GGNCC	5 cut(s)	251, 406, 518, 1352, 1575
PstI	CTGCAG	1 cut(s)	615
PsuI	RGATCY	1 cut(s)	1703
RruI	TCGCGA	1 cut(s)	603
RseI	CAYNNNNRTG	5 cut(s)	192, 556, 1056, 1683, 1835
SacI	GAGCTC	2 cut(s)	726, 781
SaqAI	TTAA	4 cut(s)	141, 326, 360, 797
SatI	GCNGC	3 cut(s)	614, 671, 1049
Sau3AI	GATC	4 cut(s)	444, 598, 1703, 1849
Sau96I	GGNCC	5 cut(s)	251, 406, 518, 1352, 1575
SchI	GAGTC	3 cut(s)	91, 1081, 1145
ScrFI	CCNGG	3 cut(s)	750, 972, 1790
SduI	GDGCHC	6 cut(s)	199, 324, 726, 781, 1075, 1560
SexAI	ACCWGGT	1 cut(s)	1788
SfaNI	GCATC	4 cut(s)	224, 277, 1060, 1105
SfcI	CTRYAG	3 cut(s)	462, 611, 1643
Sfr274I	CTCGAG	1 cut(s)	410
SfuI	TTCGAA	2 cut(s)	53, 68
SinI	GGWCC	3 cut(s)	251, 518, 1352
SlaI	CTCGAG	1 cut(s)	410
SmiMI	CAYNNNNRTG	5 cut(s)	192, 556, 1056, 1683, 1835
SmlI	CTYRAG	4 cut(s)	140, 218, 410, 1157
SmoI	CTYRAG	4 cut(s)	140, 218, 410, 1157
SphI	GCATGC	1 cut(s)	896
SseBI	AGGCCT	3 cut(s)	118, 1000, 1495
SspI	AATATT	1 cut(s)	821
SspMI	CTAG	4 cut(s)	192, 1076, 1757, 1806
SstI	GAGCTC	2 cut(s)	726, 781
StuI	AGGCCT	3 cut(s)	118, 1000, 1495
StyD4I	CCNGG	3 cut(s)	748, 970, 1788
StyI	CCWWGG	2 cut(s)	1246, 1316
TaaI	ACNGT	6 cut(s)	134, 372, 481, 968, 980, 1834
TaiI	ACGT	2 cut(s)	1597, 1755
TaqI	TCGA	6 cut(s)	53, 68, 411, 1054, 1170, 1449
TfiI	GAWTC	3 cut(s)	338, 1101, 1660
Tru1I	TTAA	4 cut(s)	141, 326, 360, 797
Tru9I	TTAA	4 cut(s)	141, 326, 360, 797
TscAI	CASTG	5 cut(s)	97, 377, 983, 1072, 1406
TseFI	GTSAC	5 cut(s)	128, 173, 734, 974, 1828
TseI	GCWGC	3 cut(s)	613, 670, 1048
Tsp45I	GTSAC	5 cut(s)	128, 173, 734, 974, 1828
TspDTI	ATGAA	7 cut(s)	796, 831, 977, 1165, 1167, 1344, 1836
TspGWI	ACGGA	3 cut(s)	789, 1672, 1696
TspRI	CASTG	5 cut(s)	97, 377, 983, 1072, 1406
Vha464I	CTTAAG	1 cut(s)	140
VneI	GTGCAC	1 cut(s)	1071
VpaK11BI	GGWCC	3 cut(s)	251, 518, 1352
VspI	ATTAAT	1 cut(s)	797
XagI	CCTNNNNNAGG	1 cut(s)	583
XapI	RAATTY	3 cut(s)	431, 1037, 1394
XceI	RCATGY	1 cut(s)	896
XhoI	CTCGAG	1 cut(s)	410
XmiI	GTMKAC	3 cut(s)	483, 1184, 1546
XmnI	GAANNNNTTC	1 cut(s)	1659
XspI	CTAG	4 cut(s)	192, 1076, 1757, 1806
Zsp2I	ATGCAT	1 cut(s)	292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.