threonine-tRNA ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1083, 1103, 1485 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 140 |
| Acc16I | TGCGCA | 1 cut(s) | 1699 |
| AccB1I | GGYRCC | 1 cut(s) | 148 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 967 |
| AccI | GTMKAC | 1 cut(s) | 1478 |
| AccII | CGCG | 2 cut(s) | 170, 1386 |
| AciI | CCGC | 4 cut(s) | 61, 241, 285, 638 |
| AclWI | GGATC | 2 cut(s) | 254, 1950 |
| AcoI | YGGCCR | 1 cut(s) | 29 |
| AcsI | RAATTY | 2 cut(s) | 1238, 1733 |
| AcuI | CTGAAG | 2 cut(s) | 852, 1532 |
| AcyI | GRCGYC | 2 cut(s) | 149, 359 |
| AfaI | GTAC | 5 cut(s) | 1006, 1157, 1389, 2010, 2033 |
| AfeI | AGCGCT | 1 cut(s) | 367 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 415, 419, 879, 967 |
| AflII | CTTAAG | 1 cut(s) | 434 |
| AflIII | ACRYGT | 3 cut(s) | 168, 1152, 1384 |
| AjiI | CACGTC | 1 cut(s) | 1883 |
| AjnI | CCWGG | 4 cut(s) | 26, 219, 1042, 1264 |
| Alw21I | GWGCWC | 4 cut(s) | 493, 554, 1020, 1369 |
| Alw26I | GTCTC | 3 cut(s) | 1774, 1909, 2091 |
| Alw44I | GTGCAC | 2 cut(s) | 550, 1365 |
| AlwI | GGATC | 2 cut(s) | 254, 1950 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 967 |
| Ama87I | CYCGRG | 1 cut(s) | 176 |
| Aor51HI | AGCGCT | 1 cut(s) | 367 |
| AoxI | GGCC | 8 cut(s) | 29, 180, 213, 787, 812, 1356, 1862, 2151 |
| ApaI | GGGCCC | 1 cut(s) | 816 |
| ApaLI | GTGCAC | 2 cut(s) | 550, 1365 |
| ApeKI | GCWGC | 7 cut(s) | 4, 38, 41, 229, 949, 1190, 1574 |
| ApoI | RAATTY | 2 cut(s) | 1238, 1733 |
| AspLEI | GCGC | 4 cut(s) | 151, 198, 368, 1700 |
| AspS9I | GGNCC | 8 cut(s) | 10, 391, 545, 788, 812, 813, 1646, 1863 |
| AsuC2I | CCSGG | 1 cut(s) | 282 |
| AsuHPI | GGTGA | 7 cut(s) | 325, 605, 1022, 1137, 2092, 2104, 2104 |
| AsuII | TTCGAA | 1 cut(s) | 347 |
| AvaI | CYCGRG | 1 cut(s) | 176 |
| AvaII | GGWCC | 4 cut(s) | 10, 391, 545, 1646 |
| BaeGI | GKGCMC | 3 cut(s) | 554, 816, 1369 |
| BalI | TGGCCA | 1 cut(s) | 31 |
| BanI | GGYRCC | 1 cut(s) | 148 |
| BanII | GRGCYC | 2 cut(s) | 816, 1020 |
| BbsI | GAAGAC | 1 cut(s) | 459 |
| Bbv12I | GWGCWC | 4 cut(s) | 493, 554, 1020, 1369 |
| BbvCI | CCTCAGC | 2 cut(s) | 634, 1569 |
| BbvI | GCAGC | 7 cut(s) | 16, 50, 53, 241, 961, 1177, 1561 |
| BccI | CCATC | 3 cut(s) | 224, 1625, 1959 |
| BceAI | ACGGC | 4 cut(s) | 48, 125, 167, 393 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1277, 1311 |
| BciT130I | CCWGG | 4 cut(s) | 28, 221, 1044, 1266 |
| BciVI | GTATCC | 1 cut(s) | 1958 |
| BcnI | CCSGG | 1 cut(s) | 282 |
| BcoDI | GTCTC | 3 cut(s) | 1774, 1909, 2091 |
| BfaI | CTAG | 2 cut(s) | 486, 558 |
| BfmI | CTRYAG | 2 cut(s) | 1706, 1752 |
| BfoI | RGCGCY | 2 cut(s) | 152, 369 |
| BfrI | CTTAAG | 1 cut(s) | 434 |
| BfuI | GTATCC | 1 cut(s) | 1958 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 146, 221, 638, 1870 |
| BglII | AGATCT | 1 cut(s) | 1546 |
| BisI | GCNGC | 8 cut(s) | 5, 39, 42, 230, 639, 950, 1191, 1575 |
| BlpI | GCTNAGC | 1 cut(s) | 225 |
| BlsI | GCNGC | 8 cut(s) | 6, 40, 43, 231, 640, 951, 1192, 1576 |
| Bme1390I | CCNGG | 5 cut(s) | 28, 221, 282, 1044, 1266 |
| Bme18I | GGWCC | 4 cut(s) | 10, 391, 545, 1646 |
| BmeT110I | CYCGRG | 1 cut(s) | 176 |
| BmgBI | CACGTC | 1 cut(s) | 1883 |
| BmgT120I | GGNCC | 8 cut(s) | 10, 391, 545, 788, 812, 813, 1646, 1863 |
| BmiI | GGNNCC | 7 cut(s) | 12, 150, 392, 790, 814, 815, 1813 |
| BmrFI | CCNGG | 5 cut(s) | 28, 221, 282, 1044, 1266 |
| BmsI | GCATC | 7 cut(s) | 207, 518, 571, 1354, 1399, 1576, 1663 |
| BoxI | GACNNNNGTC | 1 cut(s) | 804 |
| BpiI | GAAGAC | 1 cut(s) | 459 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 397, 429, 698, 730 |
| BpmI | CTGGAG | 3 cut(s) | 898, 1952, 2123 |
| Bpu10I | CCTNAGC | 2 cut(s) | 634, 1569 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 225 |
| Bpu14I | TTCGAA | 1 cut(s) | 347 |
| BpuEI | CTTGAG | 2 cut(s) | 533, 725 |
| BpuMI | CCSGG | 1 cut(s) | 282 |
| BsaHI | GRCGYC | 2 cut(s) | 149, 359 |
| BsaI | GGTCTC | 1 cut(s) | 1774 |
| BsaJI | CCNNGG | 6 cut(s) | 336, 792, 1042, 1264, 1265, 1610 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 59, 88, 89, 118 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 415, 419, 879, 967 |
| Bse1I | ACTGG | 7 cut(s) | 244, 881, 1329, 1359, 1378, 1474, 1693 |
| Bse3DI | GCAATG | 3 cut(s) | 330, 627, 1357 |
| BseBI | CCWGG | 4 cut(s) | 28, 221, 1044, 1266 |
| BseDI | CCNNGG | 6 cut(s) | 336, 792, 1042, 1264, 1265, 1610 |
| BseGI | GGATG | 5 cut(s) | 216, 533, 1411, 1470, 1636 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 415, 419, 879, 967 |
| BseMI | GCAATG | 3 cut(s) | 330, 627, 1357 |
| BseMII | CTCAG | 5 cut(s) | 239, 648, 1560, 1920, 2033 |
| BseNI | ACTGG | 7 cut(s) | 244, 881, 1329, 1359, 1378, 1474, 1693 |
| BseRI | GAGGAG | 5 cut(s) | 76, 93, 96, 99, 576 |
| BseSI | GKGCMC | 3 cut(s) | 554, 816, 1369 |
| BseXI | GCAGC | 7 cut(s) | 16, 50, 53, 241, 961, 1177, 1561 |
| BseYI | CCCAGC | 1 cut(s) | 120 |
| Bsh1236I | CGCG | 2 cut(s) | 170, 1386 |
| BshFI | GGCC | 8 cut(s) | 31, 182, 215, 789, 814, 1358, 1864, 2153 |
| BshNI | GGYRCC | 1 cut(s) | 148 |
| BsiHKAI | GWGCWC | 4 cut(s) | 493, 554, 1020, 1369 |
| BsiHKCI | CYCGRG | 1 cut(s) | 176 |
| BsiSI | CCGG | 2 cut(s) | 281, 755 |
| BsiWI | CGTACG | 1 cut(s) | 1387 |
| BslFI | GGGAC | 5 cut(s) | 232, 404, 413, 437, 1417 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 415, 419, 879, 967 |
| BsmAI | GTCTC | 3 cut(s) | 1774, 1909, 2091 |
| BsmFI | GGGAC | 5 cut(s) | 232, 404, 413, 437, 1417 |
| BsmI | GAATGC | 1 cut(s) | 481 |
| BsnI | GGCC | 8 cut(s) | 31, 182, 215, 789, 814, 1358, 1864, 2153 |
| Bso31I | GGTCTC | 1 cut(s) | 1774 |
| BsoBI | CYCGRG | 1 cut(s) | 176 |
| Bsp119I | TTCGAA | 1 cut(s) | 347 |
| Bsp120I | GGGCCC | 1 cut(s) | 812 |
| Bsp1286I | GDGCHC | 5 cut(s) | 493, 554, 816, 1020, 1369 |
| Bsp1407I | TGTACA | 1 cut(s) | 1155 |
| Bsp143I | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 225 |
| BspACI | CCGC | 4 cut(s) | 61, 241, 285, 638 |
| BspANI | GGCC | 8 cut(s) | 31, 182, 215, 789, 814, 1358, 1864, 2153 |
| BspCNI | CTCAG | 5 cut(s) | 238, 647, 1561, 1919, 2032 |
| BspFNI | CGCG | 2 cut(s) | 170, 1386 |
| BspLI | GGNNCC | 7 cut(s) | 12, 150, 392, 790, 814, 815, 1813 |
| BspMAI | CTGCAG | 1 cut(s) | 1756 |
| BspPI | GGATC | 2 cut(s) | 254, 1950 |
| BspQI | GCTCTTC | 3 cut(s) | 212, 344, 446 |
| BspT104I | TTCGAA | 1 cut(s) | 347 |
| BspT107I | GGYRCC | 1 cut(s) | 148 |
| BspTI | CTTAAG | 1 cut(s) | 434 |
| BspTNI | GGTCTC | 1 cut(s) | 1774 |
| BsrDI | GCAATG | 3 cut(s) | 330, 627, 1357 |
| BsrGI | TGTACA | 1 cut(s) | 1155 |
| BsrI | ACTGG | 7 cut(s) | 244, 881, 1329, 1359, 1378, 1474, 1693 |
| BssECI | CCNNGG | 6 cut(s) | 336, 792, 1042, 1264, 1265, 1610 |
| BssMI | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| BssNAI | GTATAC | 1 cut(s) | 1479 |
| BssNI | GRCGYC | 2 cut(s) | 149, 359 |
| BssT1I | CCWWGG | 3 cut(s) | 336, 792, 1610 |
| Bst1107I | GTATAC | 1 cut(s) | 1479 |
| Bst2UI | CCWGG | 4 cut(s) | 28, 221, 1044, 1266 |
| Bst4CI | ACNGT | 8 cut(s) | 137, 428, 593, 775, 999, 1839, 2031, 2116 |
| Bst6I | CTCTTC | 5 cut(s) | 117, 212, 299, 344, 446 |
| BstACI | GRCGYC | 2 cut(s) | 149, 359 |
| BstAFI | CTTAAG | 1 cut(s) | 434 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 537 |
| BstAUI | TGTACA | 1 cut(s) | 1155 |
| BstBI | TTCGAA | 1 cut(s) | 347 |
| BstC8I | GCNNGC | 3 cut(s) | 234, 533, 1607 |
| BstDEI | CTNAG | 9 cut(s) | 225, 634, 1037, 1319, 1462, 1569, 1874, 1906, 2019 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 417, 877 |
| BstF5I | GGATG | 5 cut(s) | 216, 533, 1411, 1470, 1636 |
| BstFNI | CGCG | 2 cut(s) | 170, 1386 |
| BstH2I | RGCGCY | 2 cut(s) | 152, 369 |
| BstHHI | GCGC | 4 cut(s) | 151, 198, 368, 1700 |
| BstKTI | GATC | 5 cut(s) | 262, 751, 895, 1549, 1945 |
| BstMAI | GTCTC | 3 cut(s) | 1774, 1909, 2091 |
| BstMBI | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| BstNI | CCWGG | 4 cut(s) | 28, 221, 1044, 1266 |
| BstNSI | RCATGY | 2 cut(s) | 855, 1156 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 804 |
| BstSCI | CCNGG | 5 cut(s) | 26, 219, 280, 1042, 1264 |
| BstSFI | CTRYAG | 2 cut(s) | 1706, 1752 |
| BstSLI | GKGCMC | 3 cut(s) | 554, 816, 1369 |
| BstUI | CGCG | 2 cut(s) | 170, 1386 |
| BstV1I | GCAGC | 7 cut(s) | 16, 50, 53, 241, 961, 1177, 1561 |
| BstV2I | GAAGAC | 1 cut(s) | 459 |
| BstX2I | RGATCY | 1 cut(s) | 1546 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1050, 1958 |
| BstYI | RGATCY | 1 cut(s) | 1546 |
| BstZ17I | GTATAC | 1 cut(s) | 1479 |
| BsuI | GTATCC | 1 cut(s) | 1958 |
| BsuRI | GGCC | 8 cut(s) | 31, 182, 215, 789, 814, 1358, 1864, 2153 |
| BtgZI | GCGATG | 1 cut(s) | 1331 |
| BtrI | CACGTC | 1 cut(s) | 1883 |
| BtsCI | GGATG | 5 cut(s) | 216, 533, 1411, 1470, 1636 |
| BtsIMutI | CAGTG | 6 cut(s) | 142, 1366, 1367, 1669, 1676, 1700 |
| Cac8I | GCNNGC | 3 cut(s) | 234, 533, 1607 |
| CaiI | CAGNNNCTG | 1 cut(s) | 967 |
| CfoI | GCGC | 4 cut(s) | 151, 198, 368, 1700 |
| Cfr13I | GGNCC | 8 cut(s) | 10, 391, 545, 788, 812, 813, 1646, 1863 |
| CseI | GACGC | 2 cut(s) | 176, 348 |
| Csp6I | GTAC | 5 cut(s) | 1005, 1156, 1388, 2009, 2032 |
| CviAII | CATG | 7 cut(s) | 145, 403, 852, 1153, 1187, 1540, 1557 |
| CviQI | GTAC | 5 cut(s) | 1005, 1156, 1388, 2009, 2032 |
| DdeI | CTNAG | 9 cut(s) | 225, 634, 1037, 1319, 1462, 1569, 1874, 1906, 2019 |
| DinI | GGCGCC | 1 cut(s) | 150 |
| DpnI | GATC | 5 cut(s) | 261, 750, 894, 1548, 1944 |
| DpnII | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 140 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 140 |
| EaeI | YGGCCR | 1 cut(s) | 29 |
| Eam1104I | CTCTTC | 5 cut(s) | 117, 212, 299, 344, 446 |
| EarI | CTCTTC | 5 cut(s) | 117, 212, 299, 344, 446 |
| EciI | GGCGGA | 2 cut(s) | 50, 300 |
| Ecl136II | GAGCTC | 1 cut(s) | 1018 |
| Eco130I | CCWWGG | 3 cut(s) | 336, 792, 1610 |
| Eco24I | GRGCYC | 2 cut(s) | 816, 1020 |
| Eco31I | GGTCTC | 1 cut(s) | 1774 |
| Eco32I | GATATC | 1 cut(s) | 1660 |
| Eco47I | GGWCC | 4 cut(s) | 10, 391, 545, 1646 |
| Eco47III | AGCGCT | 1 cut(s) | 367 |
| Eco53kI | GAGCTC | 1 cut(s) | 1018 |
| Eco57I | CTGAAG | 2 cut(s) | 852, 1532 |
| Eco88I | CYCGRG | 1 cut(s) | 176 |
| EcoICRI | GAGCTC | 1 cut(s) | 1018 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 417, 877 |
| EcoO109I | RGGNCCY | 2 cut(s) | 391, 813 |
| EcoRII | CCWGG | 4 cut(s) | 26, 219, 1042, 1264 |
| EcoRV | GATATC | 1 cut(s) | 1660 |
| EcoT14I | CCWWGG | 3 cut(s) | 336, 792, 1610 |
| EcoT22I | ATGCAT | 1 cut(s) | 1591 |
| EcoT38I | GRGCYC | 2 cut(s) | 816, 1020 |
| EgeI | GGCGCC | 1 cut(s) | 150 |
| EheI | GGCGCC | 1 cut(s) | 150 |
| ErhI | CCWWGG | 3 cut(s) | 336, 792, 1610 |
| FaeI | CATG | 7 cut(s) | 148, 406, 855, 1156, 1190, 1543, 1560 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1445, 1477 |
| FaqI | GGGAC | 5 cut(s) | 232, 404, 413, 437, 1417 |
| FatI | CATG | 7 cut(s) | 144, 402, 851, 1152, 1186, 1539, 1556 |
| FblI | GTMKAC | 1 cut(s) | 1478 |
| Fnu4HI | GCNGC | 8 cut(s) | 5, 39, 42, 230, 639, 950, 1191, 1575 |
| FokI | GGATG | 5 cut(s) | 203, 540, 1418, 1457, 1643 |
| FriOI | GRGCYC | 2 cut(s) | 816, 1020 |
| Fsp4HI | GCNGC | 8 cut(s) | 5, 39, 42, 230, 639, 950, 1191, 1575 |
| FspBI | CTAG | 2 cut(s) | 486, 558 |
| FspI | TGCGCA | 1 cut(s) | 1699 |
| GlaI | GCGC | 4 cut(s) | 150, 197, 367, 1699 |
| GluI | GCNGC | 8 cut(s) | 5, 39, 42, 230, 639, 950, 1191, 1575 |
| GsaI | CCCAGC | 1 cut(s) | 124 |
| GsuI | CTGGAG | 3 cut(s) | 898, 1952, 2123 |
| HaeII | RGCGCY | 2 cut(s) | 152, 369 |
| HaeIII | GGCC | 8 cut(s) | 31, 182, 215, 789, 814, 1358, 1864, 2153 |
| HapII | CCGG | 2 cut(s) | 281, 755 |
| HgaI | GACGC | 2 cut(s) | 176, 348 |
| HhaI | GCGC | 4 cut(s) | 151, 198, 368, 1700 |
| Hin1I | GRCGYC | 2 cut(s) | 149, 359 |
| Hin1II | CATG | 7 cut(s) | 148, 406, 855, 1156, 1190, 1543, 1560 |
| Hin6I | GCGC | 4 cut(s) | 149, 196, 366, 1698 |
| HinP1I | GCGC | 4 cut(s) | 149, 196, 366, 1698 |
| HincII | GTYRAC | 2 cut(s) | 799, 1978 |
| HindII | GTYRAC | 2 cut(s) | 799, 1978 |
| HinfI | GANTC | 3 cut(s) | 912, 1395, 1430 |
| HpaII | CCGG | 2 cut(s) | 281, 755 |
| HphI | GGTGA | 7 cut(s) | 325, 605, 1022, 1137, 2092, 2104, 2104 |
| Hpy166II | GTNNAC | 8 cut(s) | 552, 662, 799, 1367, 1479, 1880, 1978, 2075 |
| Hpy188I | TCNGA | 8 cut(s) | 259, 503, 718, 1077, 1672, 1909, 2001, 2022 |
| Hpy8I | GTNNAC | 8 cut(s) | 552, 662, 799, 1367, 1479, 1880, 1978, 2075 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 9 cut(s) | 260, 289, 293, 413, 455, 853, 1619, 1760, 1876 |
| HpyCH4III | ACNGT | 8 cut(s) | 137, 428, 593, 775, 999, 1839, 2031, 2116 |
| HpyCH4IV | ACGT | 1 cut(s) | 1882 |
| HpyF3I | CTNAG | 9 cut(s) | 225, 634, 1037, 1319, 1462, 1569, 1874, 1906, 2019 |
| HpySE526I | ACGT | 1 cut(s) | 1882 |
| Hsp92I | GRCGYC | 2 cut(s) | 149, 359 |
| Hsp92II | CATG | 7 cut(s) | 148, 406, 855, 1156, 1190, 1543, 1560 |
| HspAI | GCGC | 4 cut(s) | 149, 196, 366, 1698 |
| KasI | GGCGCC | 1 cut(s) | 148 |
| Kzo9I | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| LguI | GCTCTTC | 3 cut(s) | 212, 344, 446 |
| LmnI | GCTCC | 2 cut(s) | 1015, 1817 |
| Lsp1109I | GCAGC | 7 cut(s) | 16, 50, 53, 241, 961, 1177, 1561 |
| LweI | GCATC | 7 cut(s) | 207, 518, 571, 1354, 1399, 1576, 1663 |
| MaeI | CTAG | 2 cut(s) | 486, 558 |
| MaeII | ACGT | 1 cut(s) | 1882 |
| MaeIII | GTNAC | 5 cut(s) | 65, 313, 685, 1028, 2110 |
| MalI | GATC | 5 cut(s) | 261, 750, 894, 1548, 1944 |
| MboI | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| MfeI | CAATTG | 1 cut(s) | 1890 |
| MflI | RGATCY | 1 cut(s) | 1546 |
| MhlI | GDGCHC | 5 cut(s) | 493, 554, 816, 1020, 1369 |
| MlsI | TGGCCA | 1 cut(s) | 31 |
| MluCI | AATT | 7 cut(s) | 725, 1195, 1238, 1258, 1733, 1744, 1890 |
| MluI | ACGCGT | 2 cut(s) | 168, 1384 |
| MluNI | TGGCCA | 1 cut(s) | 31 |
| Mly113I | GGCGCC | 1 cut(s) | 149 |
| MlyI | GAGTC | 2 cut(s) | 921, 1439 |
| MmeI | TCCRAC | 2 cut(s) | 1755, 1821 |
| Mox20I | TGGCCA | 1 cut(s) | 31 |
| Mph1103I | ATGCAT | 1 cut(s) | 1591 |
| MscI | TGGCCA | 1 cut(s) | 31 |
| MseI | TTAA | 4 cut(s) | 435, 654, 1379, 1592 |
| MslI | CAYNNNNRTG | 4 cut(s) | 486, 850, 1350, 2117 |
| Msp20I | TGGCCA | 1 cut(s) | 31 |
| MspA1I | CMGCKG | 2 cut(s) | 7, 638 |
| MspCI | CTTAAG | 1 cut(s) | 434 |
| MspI | CCGG | 2 cut(s) | 281, 755 |
| MspR9I | CCNGG | 5 cut(s) | 28, 221, 282, 1044, 1266 |
| MunI | CAATTG | 1 cut(s) | 1890 |
| Mva1269I | GAATGC | 1 cut(s) | 481 |
| MvaI | CCWGG | 4 cut(s) | 28, 221, 1044, 1266 |
| MvnI | CGCG | 2 cut(s) | 170, 1386 |
| NarI | GGCGCC | 1 cut(s) | 149 |
| NciI | CCSGG | 1 cut(s) | 282 |
| NdeII | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| NlaIII | CATG | 7 cut(s) | 148, 406, 855, 1156, 1190, 1543, 1560 |
| NlaIV | GGNNCC | 7 cut(s) | 12, 150, 392, 790, 814, 815, 1813 |
| NmuCI | GTSAC | 4 cut(s) | 313, 685, 1028, 2110 |
| NsbI | TGCGCA | 1 cut(s) | 1699 |
| NsiI | ATGCAT | 1 cut(s) | 1591 |
| NspI | RCATGY | 2 cut(s) | 855, 1156 |
| NspV | TTCGAA | 1 cut(s) | 347 |
| PaeR7I | CTCGAG | 1 cut(s) | 176 |
| PasI | CCCWGGG | 1 cut(s) | 1265 |
| PciI | ACATGT | 1 cut(s) | 1152 |
| PciSI | GCTCTTC | 3 cut(s) | 212, 344, 446 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 174 |
| PctI | GAATGC | 1 cut(s) | 481 |
| PfeI | GAWTC | 1 cut(s) | 1395 |
| Pfl23II | CGTACG | 1 cut(s) | 1387 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 967 |
| PkrI | GCNGC | 8 cut(s) | 6, 40, 43, 231, 640, 951, 1192, 1576 |
| PleI | GAGTC | 2 cut(s) | 920, 1438 |
| PluTI | GGCGCC | 1 cut(s) | 152 |
| PpsI | GAGTC | 2 cut(s) | 920, 1438 |
| PpuMI | RGGWCCY | 1 cut(s) | 391 |
| PscI | ACATGT | 1 cut(s) | 1152 |
| PshAI | GACNNNNGTC | 1 cut(s) | 804 |
| PsiI | TTATAA | 3 cut(s) | 1083, 1103, 1485 |
| Psp124BI | GAGCTC | 1 cut(s) | 1020 |
| Psp5II | RGGWCCY | 1 cut(s) | 391 |
| Psp6I | CCWGG | 4 cut(s) | 26, 219, 1042, 1264 |
| PspFI | CCCAGC | 1 cut(s) | 120 |
| PspGI | CCWGG | 4 cut(s) | 26, 219, 1042, 1264 |
| PspLI | CGTACG | 1 cut(s) | 1387 |
| PspN4I | GGNNCC | 7 cut(s) | 12, 150, 392, 790, 814, 815, 1813 |
| PspOMI | GGGCCC | 1 cut(s) | 812 |
| PspPI | GGNCC | 8 cut(s) | 10, 391, 545, 788, 812, 813, 1646, 1863 |
| PspPPI | RGGWCCY | 1 cut(s) | 391 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1673, 1705 |
| PstI | CTGCAG | 1 cut(s) | 1756 |
| PstNI | CAGNNNCTG | 1 cut(s) | 967 |
| PsuI | RGATCY | 1 cut(s) | 1546 |
| PvuII | CAGCTG | 1 cut(s) | 7 |
| RsaI | GTAC | 5 cut(s) | 1006, 1157, 1389, 2010, 2033 |
| RsaNI | GTAC | 5 cut(s) | 1005, 1156, 1388, 2009, 2032 |
| RseI | CAYNNNNRTG | 4 cut(s) | 486, 850, 1350, 2117 |
| SacI | GAGCTC | 1 cut(s) | 1020 |
| SapI | GCTCTTC | 3 cut(s) | 212, 344, 446 |
| SaqAI | TTAA | 4 cut(s) | 435, 654, 1379, 1592 |
| SatI | GCNGC | 8 cut(s) | 5, 39, 42, 230, 639, 950, 1191, 1575 |
| Sau3AI | GATC | 5 cut(s) | 259, 748, 892, 1546, 1942 |
| Sau96I | GGNCC | 8 cut(s) | 10, 391, 545, 788, 812, 813, 1646, 1863 |
| SchI | GAGTC | 2 cut(s) | 921, 1439 |
| ScrFI | CCNGG | 5 cut(s) | 28, 221, 282, 1044, 1266 |
| SduI | GDGCHC | 5 cut(s) | 493, 554, 816, 1020, 1369 |
| SfaNI | GCATC | 7 cut(s) | 207, 518, 571, 1354, 1399, 1576, 1663 |
| SfcI | CTRYAG | 2 cut(s) | 1706, 1752 |
| SfoI | GGCGCC | 1 cut(s) | 150 |
| Sfr274I | CTCGAG | 1 cut(s) | 176 |
| SfuI | TTCGAA | 1 cut(s) | 347 |
| SinI | GGWCC | 4 cut(s) | 10, 391, 545, 1646 |
| SlaI | CTCGAG | 1 cut(s) | 176 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 486, 850, 1350, 2117 |
| SmlI | CTYRAG | 4 cut(s) | 176, 434, 512, 704 |
| SmoI | CTYRAG | 4 cut(s) | 176, 434, 512, 704 |
| Sse9I | AATT | 7 cut(s) | 725, 1195, 1238, 1258, 1733, 1744, 1890 |
| SsiI | CCGC | 4 cut(s) | 61, 241, 285, 638 |
| SspDI | GGCGCC | 1 cut(s) | 148 |
| SspMI | CTAG | 2 cut(s) | 486, 558 |
| SstI | GAGCTC | 1 cut(s) | 1020 |
| StyD4I | CCNGG | 5 cut(s) | 26, 219, 280, 1042, 1264 |
| StyI | CCWWGG | 3 cut(s) | 336, 792, 1610 |
| TaaI | ACNGT | 8 cut(s) | 137, 428, 593, 775, 999, 1839, 2031, 2116 |
| TaiI | ACGT | 1 cut(s) | 1885 |
| TaqI | TCGA | 6 cut(s) | 158, 177, 210, 347, 1506, 1737 |
| TasI | AATT | 7 cut(s) | 725, 1195, 1238, 1258, 1733, 1744, 1890 |
| TatI | WGTACW | 2 cut(s) | 1155, 2031 |
| TauI | GCSGC | 1 cut(s) | 641 |
| TfiI | GAWTC | 1 cut(s) | 1395 |
| Tru1I | TTAA | 4 cut(s) | 435, 654, 1379, 1592 |
| Tru9I | TTAA | 4 cut(s) | 435, 654, 1379, 1592 |
| TscAI | CASTG | 6 cut(s) | 142, 1366, 1374, 1669, 1683, 1700 |
| TseFI | GTSAC | 4 cut(s) | 313, 685, 1028, 2110 |
| TseI | GCWGC | 7 cut(s) | 4, 38, 41, 229, 949, 1190, 1574 |
| Tsp45I | GTSAC | 4 cut(s) | 313, 685, 1028, 2110 |
| TspDTI | ATGAA | 5 cut(s) | 474, 1271, 1459, 1461, 1638 |
| TspGWI | ACGGA | 4 cut(s) | 146, 537, 1405, 1804 |
| TspRI | CASTG | 6 cut(s) | 142, 1366, 1374, 1669, 1683, 1700 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 967 |
| Vha464I | CTTAAG | 1 cut(s) | 434 |
| VneI | GTGCAC | 2 cut(s) | 550, 1365 |
| VpaK11BI | GGWCC | 4 cut(s) | 10, 391, 545, 1646 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 417, 877 |
| XapI | RAATTY | 2 cut(s) | 1238, 1733 |
| XceI | RCATGY | 2 cut(s) | 855, 1156 |
| XhoI | CTCGAG | 1 cut(s) | 176 |
| XmiI | GTMKAC | 1 cut(s) | 1478 |
| XspI | CTAG | 2 cut(s) | 486, 558 |
| Zsp2I | ATGCAT | 1 cut(s) | 1591 |