Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Reverse (-)

6232763 .. 6241566

Gene Structure 8804 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1089000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGCAGCTGGTCTCCCTTTCTAAAACCTGGCCGCCAGCAGCAGCAGCTATCTCTTTCTTTCTCCGCCGTAACTCCTACTCCCCATTCTCCTCACTCTCCACTTCTCCTCCTCCTCTTCTTCCCCAGCTCCCTACGGACAGCGCCGCCATGGCGCCCCACCCGAAAGACGACGCGTATCTCGAGGCCGTAATCCCGAAGCGCATCAAGCTCTTCGAGGCCATCCAGGCCCAGCAGCTCGCCCACCGCCAGTCCCTCCCCTCCGATCCAATCAAGATTACTTTGCCGGGCGGACAGGAGAAGGAAGGGAAGAGGTGGGTGACTTCGCCATTTGACATTGCCAAGGATATTTCCAAGAGCTTGGCTTCCAGCGCTGTGATTGCAAAGGTCAATGGGGACCTATGGGACATGGGCCGTCCTCTGGAAGGGGACTGTGAGCTTAAGCTCTTCAAGTTTGAAGACGATGAAGGGCGTGATACTTTCTGGCATTCTAGTGCTCACATTCTCGGACAGGCACTTGAGGTTGAGTATGGATGCAAGCTCTGCATTGGTCCGTGCACTACTAGAGGAGAGGGTTTCTATTACGATGCGTTCTACGGCGATTTGGGCTTGAATGACGAACACTTCAAGGACATTGCATCAGCGGCAGATAAGGCTGTTAAGGGTAAACAACCTTTTGAGCGTATTGAAGTCACAAGAGAACAAGCTCTTGAGCTTTTCTCTGATAATCAATTCAAGGTTGAAATCATCAATGATCTTCCGGCAGAGAAAACTATAACAGTATATAGATGTGGCCCCTTGGTTGACTTGTGTCGTGGGCCCCATATACCAAATACTTCTTTTGTCAAAGCATTTGCATGTTTGAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGTGAAAGTTTACAGAGAGTCTATGGAATATCATATCCTGATAAGAAAAGTTTGCAGCTATATATCCAGAAGCTGGAAGAAGCAAAAAAGTATGACCACAGACTGTTGGGTACAAAGCAGGAGCTCTTTTTTTGTCACCCACTTAGCCCAGGAAGTTGGTTCTTCCTCCCTAATGGAACTCGGATTTATAACAAACTAATGGAGTTTATAAAAAATCAATATAGAGAACGAGGCTATAAAGAGGTTATATCACCAAACATGTACAATATGCAACTTTGGGAAACATCTGGTCATGCTGCTAATTACAAAGAGAATATGTTTGTATTTGAGATTGAGAAACAAGAATTTGGATTGAAGCCAATGAATTGCCCAGGGCATTGTTTGATGTTTCAGCATAGGGTTCGCTCGTATAGAGAACTTCCACTTAGACTGGCTGATTTTGGTGTTTTGCATCGCAATGAGGCTAGTGGTGCACTGACTGGATTAACACGAGTACGGAGATTCCAGCAGGATGATGCCCATATCTTTTGCAGGGAGTCCCAAGTGAAAGATGAAGTAAAGGGTGTCTTAGATTTCATCCGGTATACTTATAATATTTTTGGCTTTTCTTTCGATCTGAAGCTATCAACAATGCCAGAAAAACACATGGGAGATCTGGCAACATGGGAGAAAGCTGAGGCTGCTCTTGCAGACGCATTAAATGAGTTTGGCAAGCCCTGGCAGATAAATGAAGGGGATGGTGCATTTTATGGACCAAAAATAGATATCAGTGTATCTGATGCACTGAACAGAAAATATCAGTGCGCAACTCTACAGCTTGATTTTCAGCTTCCACAAAAATTCGAGCTGAATTACTCTGCAGAGGGGGAAGAAGGCAAGTTGGAGAGACCTGTTATGATACACAGAGCTGTTTTAGGCTCCGTTGAGCGTATGTTTGCTATACTGTTGGAGAACTACAAAGGAAAGTGGCCCTTCTGGCTTAGTCCACGTCAGGCAATTGTTTGCCCTGTCTCAGACAAATCCCAACCTTATGCTCTGGAGGTTCGGGATCGTATCCATCAAGCTGGTTATTATGTTGATGTTGACACTACTGATAGAACCATTCAGAAAAAGGTACGAGAAGCTCAGATAGCACAGTACAACTACATTTTAGTTGTGGGCGAGGAAGAAGTCAAGACTGGACAGGTGAGCTTACGGGTGAGGGACAAGGGAGATGTCACCGTAATGACCATGGACAATCTGCTCCAGCACTTCAAGGCCGAAGTCGAAGCGTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

726

Amino Acids

82.79

Weight (kDa)

6.55

Isoelectric Point (pI)

35.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	90 - 150	1.5e-16	TGS domain
tRNA_SAD	PF07973	258 - 307	1.8e-13	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	406 - 618	1.2e-45	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	630 - 719	6.6e-21	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1086, 1106, 1488
Acc16I	TGCGCA	1 cut(s)	1702
AccB1I	GGYRCC	1 cut(s)	151
AccB7I	CCANNNNNTGG	1 cut(s)	970
AccI	GTMKAC	1 cut(s)	1481
AccII	CGCG	1 cut(s)	173
AciI	CCGC	6 cut(s)	32, 64, 144, 244, 288, 641
AclWI	GGATC	2 cut(s)	257, 1953
AcoI	YGGCCR	1 cut(s)	29
AcsI	RAATTY	2 cut(s)	1241, 1736
AcuI	CTGAAG	2 cut(s)	855, 1535
AcyI	GRCGYC	1 cut(s)	152
AfaI	GTAC	5 cut(s)	1009, 1160, 1392, 2013, 2036
AfeI	AGCGCT	1 cut(s)	370
AfiI	CCNNNNNNNGG	4 cut(s)	418, 422, 882, 970
AflII	CTTAAG	1 cut(s)	437
AflIII	ACRYGT	2 cut(s)	171, 1155
AjiI	CACGTC	1 cut(s)	1886
AjnI	CCWGG	5 cut(s)	26, 222, 1045, 1267, 1613
Alw21I	GWGCWC	4 cut(s)	496, 557, 1023, 1372
Alw26I	GTCTC	3 cut(s)	16, 1777, 1912
Alw44I	GTGCAC	2 cut(s)	553, 1368
AlwI	GGATC	2 cut(s)	257, 1953
AlwNI	CAGNNNCTG	1 cut(s)	970
Ama87I	CYCGRG	1 cut(s)	179
Aor51HI	AGCGCT	1 cut(s)	370
AoxI	GGCC	9 cut(s)	29, 183, 216, 225, 409, 790, 815, 1865, 2154
ApaI	GGGCCC	1 cut(s)	819
ApaLI	GTGCAC	2 cut(s)	553, 1368
ApeKI	GCWGC	8 cut(s)	4, 38, 41, 44, 232, 952, 1193, 1577
ApoI	RAATTY	2 cut(s)	1241, 1736
AspLEI	GCGC	5 cut(s)	143, 154, 201, 371, 1703
AspS9I	GGNCC	9 cut(s)	226, 394, 409, 548, 791, 815, 816, 1649, 1866
AsuC2I	CCSGG	1 cut(s)	285
AsuHPI	GGTGA	6 cut(s)	328, 1025, 1140, 2095, 2107, 2107
AvaI	CYCGRG	1 cut(s)	179
AvaII	GGWCC	3 cut(s)	394, 548, 1649
BaeGI	GKGCMC	3 cut(s)	557, 819, 1372
BanI	GGYRCC	1 cut(s)	151
BanII	GRGCYC	2 cut(s)	819, 1023
BauI	CACGAG	1 cut(s)	1386
BbsI	GAAGAC	1 cut(s)	462
Bbv12I	GWGCWC	4 cut(s)	496, 557, 1023, 1372
BbvCI	CCTCAGC	1 cut(s)	1572
BbvI	GCAGC	8 cut(s)	16, 50, 53, 56, 244, 964, 1180, 1564
BccI	CCATC	3 cut(s)	227, 1628, 1962
BceAI	ACGGC	4 cut(s)	51, 170, 396, 610
BcgI	CGANNNNNNTGC	2 cut(s)	1280, 1314
BciT130I	CCWGG	5 cut(s)	28, 224, 1047, 1269, 1615
BciVI	GTATCC	1 cut(s)	1961
BcnI	CCSGG	1 cut(s)	285
BcoDI	GTCTC	3 cut(s)	16, 1777, 1912
BfaI	CTAG	3 cut(s)	489, 561, 1362
BfmI	CTRYAG	2 cut(s)	1709, 1755
BfoI	RGCGCY	3 cut(s)	144, 155, 372
BfrI	CTTAAG	1 cut(s)	437
BfuI	GTATCC	1 cut(s)	1961
BglI	GCCNNNNNGGC	3 cut(s)	149, 224, 1873
BglII	AGATCT	1 cut(s)	1549
Bme1390I	CCNGG	6 cut(s)	28, 224, 285, 1047, 1269, 1615
Bme18I	GGWCC	3 cut(s)	394, 548, 1649
BmeT110I	CYCGRG	1 cut(s)	179
BmgBI	CACGTC	1 cut(s)	1886
BmgT120I	GGNCC	9 cut(s)	226, 394, 409, 548, 791, 815, 816, 1649, 1866
BmiI	GGNNCC	6 cut(s)	153, 395, 793, 817, 818, 1816
BmrFI	CCNGG	6 cut(s)	28, 224, 285, 1047, 1269, 1615
BmsI	GCATC	7 cut(s)	210, 521, 574, 644, 1357, 1402, 1666
BoxI	GACNNNNGTC	1 cut(s)	807
BpiI	GAAGAC	1 cut(s)	462
BplI	GAGNNNNNCTC	2 cut(s)	701, 733
BpmI	CTGGAG	3 cut(s)	901, 1955, 2126
Bpu10I	CCTNAGC	1 cut(s)	1572
BpuEI	CTTGAG	2 cut(s)	536, 728
BpuMI	CCSGG	1 cut(s)	285
BsaHI	GRCGYC	1 cut(s)	152
BsaI	GGTCTC	2 cut(s)	16, 1777
BsaJI	CCNNGG	8 cut(s)	147, 339, 795, 1045, 1267, 1268, 1613, 2127
BsaWI	WCCGGW	1 cut(s)	1476
BsaXI	ACNNNNNCTCC	4 cut(s)	62, 91, 92, 121
Bsc4I	CCNNNNNNNGG	4 cut(s)	418, 422, 882, 970
Bse1I	ACTGG	5 cut(s)	247, 884, 1332, 1381, 2080
Bse3DI	GCAATG	3 cut(s)	333, 630, 1360
BseBI	CCWGG	5 cut(s)	28, 224, 1047, 1269, 1615
BseDI	CCNNGG	8 cut(s)	147, 339, 795, 1045, 1267, 1268, 1613, 2127
BseGI	GGATG	5 cut(s)	219, 536, 1414, 1473, 1639
BseLI	CCNNNNNNNGG	4 cut(s)	418, 422, 882, 970
BseMI	GCAATG	3 cut(s)	333, 630, 1360
BseMII	CTCAG	3 cut(s)	1563, 1923, 2036
BseNI	ACTGG	5 cut(s)	247, 884, 1332, 1381, 2080
BseRI	GAGGAG	5 cut(s)	79, 96, 99, 102, 579
BseSI	GKGCMC	3 cut(s)	557, 819, 1372
BseXI	GCAGC	8 cut(s)	16, 50, 53, 56, 244, 964, 1180, 1564
BseYI	CCCAGC	2 cut(s)	123, 228
Bsh1236I	CGCG	1 cut(s)	173
BshFI	GGCC	9 cut(s)	31, 185, 218, 227, 411, 792, 817, 1867, 2156
BshNI	GGYRCC	1 cut(s)	151
BsiHKAI	GWGCWC	4 cut(s)	496, 557, 1023, 1372
BsiHKCI	CYCGRG	1 cut(s)	179
BsiSI	CCGG	3 cut(s)	284, 758, 1477
BslFI	GGGAC	6 cut(s)	235, 407, 416, 440, 1420, 2114
BslI	CCNNNNNNNGG	4 cut(s)	418, 422, 882, 970
BsmAI	GTCTC	3 cut(s)	16, 1777, 1912
BsmFI	GGGAC	6 cut(s)	235, 407, 416, 440, 1420, 2114
BsmI	GAATGC	1 cut(s)	484
BsnI	GGCC	9 cut(s)	31, 185, 218, 227, 411, 792, 817, 1867, 2156
Bso31I	GGTCTC	2 cut(s)	16, 1777
BsoBI	CYCGRG	1 cut(s)	179
Bsp120I	GGGCCC	1 cut(s)	815
Bsp1286I	GDGCHC	5 cut(s)	496, 557, 819, 1023, 1372
Bsp1407I	TGTACA	1 cut(s)	1158
Bsp143I	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
Bsp19I	CCATGG	2 cut(s)	147, 2127
BspACI	CCGC	6 cut(s)	32, 64, 144, 244, 288, 641
BspANI	GGCC	9 cut(s)	31, 185, 218, 227, 411, 792, 817, 1867, 2156
BspCNI	CTCAG	3 cut(s)	1564, 1922, 2035
BspFNI	CGCG	1 cut(s)	173
BspLI	GGNNCC	6 cut(s)	153, 395, 793, 817, 818, 1816
BspMAI	CTGCAG	1 cut(s)	1759
BspPI	GGATC	2 cut(s)	257, 1953
BspQI	GCTCTTC	2 cut(s)	215, 449
BspT107I	GGYRCC	1 cut(s)	151
BspTI	CTTAAG	1 cut(s)	437
BspTNI	GGTCTC	2 cut(s)	16, 1777
BsrDI	GCAATG	3 cut(s)	333, 630, 1360
BsrGI	TGTACA	1 cut(s)	1158
BsrI	ACTGG	5 cut(s)	247, 884, 1332, 1381, 2080
BssECI	CCNNGG	8 cut(s)	147, 339, 795, 1045, 1267, 1268, 1613, 2127
BssMI	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
BssNAI	GTATAC	1 cut(s)	1482
BssNI	GRCGYC	1 cut(s)	152
BssSI	CACGAG	1 cut(s)	1386
BssT1I	CCWWGG	4 cut(s)	147, 339, 795, 2127
Bst1107I	GTATAC	1 cut(s)	1482
Bst2BI	CACGAG	1 cut(s)	1386
Bst2UI	CCWGG	5 cut(s)	28, 224, 1047, 1269, 1615
Bst4CI	ACNGT	6 cut(s)	431, 778, 1002, 1842, 2034, 2119
Bst6I	CTCTTC	4 cut(s)	120, 215, 302, 449
BstACI	GRCGYC	1 cut(s)	152
BstAFI	CTTAAG	1 cut(s)	437
BstAPI	GCANNNNNTGC	1 cut(s)	540
BstAUI	TGTACA	1 cut(s)	1158
BstC8I	GCNNGC	4 cut(s)	36, 237, 536, 1610
BstDEI	CTNAG	7 cut(s)	1040, 1322, 1465, 1572, 1877, 1909, 2022
BstDSI	CCRYGG	2 cut(s)	147, 2127
BstENI	CCTNNNNNAGG	2 cut(s)	420, 880
BstF5I	GGATG	5 cut(s)	219, 536, 1414, 1473, 1639
BstFNI	CGCG	1 cut(s)	173
BstH2I	RGCGCY	3 cut(s)	144, 155, 372
BstHHI	GCGC	5 cut(s)	143, 154, 201, 371, 1703
BstKTI	GATC	6 cut(s)	265, 754, 898, 1513, 1552, 1948
BstMAI	GTCTC	3 cut(s)	16, 1777, 1912
BstMBI	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
BstNI	CCWGG	5 cut(s)	28, 224, 1047, 1269, 1615
BstNSI	RCATGY	2 cut(s)	858, 1159
BstPAI	GACNNNNGTC	1 cut(s)	807
BstSCI	CCNGG	6 cut(s)	26, 222, 283, 1045, 1267, 1613
BstSFI	CTRYAG	2 cut(s)	1709, 1755
BstSLI	GKGCMC	3 cut(s)	557, 819, 1372
BstUI	CGCG	1 cut(s)	173
BstV1I	GCAGC	8 cut(s)	16, 50, 53, 56, 244, 964, 1180, 1564
BstV2I	GAAGAC	1 cut(s)	462
BstX2I	RGATCY	1 cut(s)	1549
BstXI	CCANNNNNNTGG	3 cut(s)	358, 1053, 1961
BstYI	RGATCY	1 cut(s)	1549
BstZ17I	GTATAC	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	1961
BsuRI	GGCC	9 cut(s)	31, 185, 218, 227, 411, 792, 817, 1867, 2156
BtgI	CCRYGG	2 cut(s)	147, 2127
BtgZI	GCGATG	1 cut(s)	1334
BtrI	CACGTC	1 cut(s)	1886
BtsCI	GGATG	5 cut(s)	219, 536, 1414, 1473, 1639
BtsIMutI	CAGTG	4 cut(s)	1370, 1672, 1679, 1703
Cac8I	GCNNGC	4 cut(s)	36, 237, 536, 1610
CaiI	CAGNNNCTG	1 cut(s)	970
CfoI	GCGC	5 cut(s)	143, 154, 201, 371, 1703
Cfr13I	GGNCC	9 cut(s)	226, 394, 409, 548, 791, 815, 816, 1649, 1866
CseI	GACGC	2 cut(s)	179, 1598
Csp6I	GTAC	5 cut(s)	1008, 1159, 1391, 2012, 2035
CviAII	CATG	8 cut(s)	148, 406, 855, 1156, 1190, 1543, 1560, 2128
CviQI	GTAC	5 cut(s)	1008, 1159, 1391, 2012, 2035
DdeI	CTNAG	7 cut(s)	1040, 1322, 1465, 1572, 1877, 1909, 2022
DinI	GGCGCC	1 cut(s)	153
DpnI	GATC	6 cut(s)	264, 753, 897, 1512, 1551, 1947
DpnII	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
EaeI	YGGCCR	1 cut(s)	29
Eam1104I	CTCTTC	4 cut(s)	120, 215, 302, 449
EarI	CTCTTC	4 cut(s)	120, 215, 302, 449
EciI	GGCGGA	2 cut(s)	53, 303
Ecl136II	GAGCTC	1 cut(s)	1021
Eco130I	CCWWGG	4 cut(s)	147, 339, 795, 2127
Eco24I	GRGCYC	2 cut(s)	819, 1023
Eco31I	GGTCTC	2 cut(s)	16, 1777
Eco32I	GATATC	1 cut(s)	1663
Eco47I	GGWCC	3 cut(s)	394, 548, 1649
Eco47III	AGCGCT	1 cut(s)	370
Eco53kI	GAGCTC	1 cut(s)	1021
Eco57I	CTGAAG	2 cut(s)	855, 1535
Eco88I	CYCGRG	1 cut(s)	179
EcoICRI	GAGCTC	1 cut(s)	1021
EcoNI	CCTNNNNNAGG	2 cut(s)	420, 880
EcoO109I	RGGNCCY	2 cut(s)	394, 816
EcoRII	CCWGG	5 cut(s)	26, 222, 1045, 1267, 1613
EcoRV	GATATC	1 cut(s)	1663
EcoT14I	CCWWGG	4 cut(s)	147, 339, 795, 2127
EcoT38I	GRGCYC	2 cut(s)	819, 1023
EgeI	GGCGCC	1 cut(s)	153
EheI	GGCGCC	1 cut(s)	153
ErhI	CCWWGG	4 cut(s)	147, 339, 795, 2127
FaeI	CATG	8 cut(s)	151, 409, 858, 1159, 1193, 1546, 1563, 2131
FalI	AAGNNNNNCTT	4 cut(s)	1448, 1480, 1853, 1885
FaqI	GGGAC	6 cut(s)	235, 407, 416, 440, 1420, 2114
FatI	CATG	8 cut(s)	147, 405, 854, 1155, 1189, 1542, 1559, 2127
FblI	GTMKAC	1 cut(s)	1481
FokI	GGATG	5 cut(s)	206, 543, 1421, 1460, 1646
FriOI	GRGCYC	2 cut(s)	819, 1023
FspBI	CTAG	3 cut(s)	489, 561, 1362
FspI	TGCGCA	1 cut(s)	1702
GlaI	GCGC	5 cut(s)	142, 153, 200, 370, 1702
GsaI	CCCAGC	2 cut(s)	127, 232
GsuI	CTGGAG	3 cut(s)	901, 1955, 2126
HaeII	RGCGCY	3 cut(s)	144, 155, 372
HaeIII	GGCC	9 cut(s)	31, 185, 218, 227, 411, 792, 817, 1867, 2156
HapII	CCGG	3 cut(s)	284, 758, 1477
HgaI	GACGC	2 cut(s)	179, 1598
HhaI	GCGC	5 cut(s)	143, 154, 201, 371, 1703
Hin1I	GRCGYC	1 cut(s)	152
Hin1II	CATG	8 cut(s)	151, 409, 858, 1159, 1193, 1546, 1563, 2131
Hin6I	GCGC	5 cut(s)	141, 152, 199, 369, 1701
HinP1I	GCGC	5 cut(s)	141, 152, 199, 369, 1701
HincII	GTYRAC	2 cut(s)	802, 1981
HindII	GTYRAC	2 cut(s)	802, 1981
HinfI	GANTC	3 cut(s)	915, 1398, 1433
HpaII	CCGG	3 cut(s)	284, 758, 1477
HphI	GGTGA	6 cut(s)	328, 1025, 1140, 2095, 2107, 2107
Hpy166II	GTNNAC	8 cut(s)	555, 665, 802, 908, 1370, 1482, 1883, 1981
Hpy188I	TCNGA	9 cut(s)	262, 506, 721, 1080, 1515, 1675, 1912, 2004, 2025
Hpy8I	GTNNAC	8 cut(s)	555, 665, 802, 908, 1370, 1482, 1883, 1981
Hpy99I	CGWCG	1 cut(s)	173
HpyAV	CCTTC	8 cut(s)	292, 296, 416, 458, 856, 1622, 1763, 1879
HpyCH4III	ACNGT	6 cut(s)	431, 778, 1002, 1842, 2034, 2119
HpyCH4IV	ACGT	1 cut(s)	1885
HpyF3I	CTNAG	7 cut(s)	1040, 1322, 1465, 1572, 1877, 1909, 2022
HpySE526I	ACGT	1 cut(s)	1885
Hsp92I	GRCGYC	1 cut(s)	152
Hsp92II	CATG	8 cut(s)	151, 409, 858, 1159, 1193, 1546, 1563, 2131
HspAI	GCGC	5 cut(s)	141, 152, 199, 369, 1701
KasI	GGCGCC	1 cut(s)	151
Kzo9I	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
LguI	GCTCTTC	2 cut(s)	215, 449
LmnI	GCTCC	4 cut(s)	132, 1018, 1820, 2145
Lsp1109I	GCAGC	8 cut(s)	16, 50, 53, 56, 244, 964, 1180, 1564
LweI	GCATC	7 cut(s)	210, 521, 574, 644, 1357, 1402, 1666
MaeI	CTAG	3 cut(s)	489, 561, 1362
MaeII	ACGT	1 cut(s)	1885
MaeIII	GTNAC	5 cut(s)	68, 316, 688, 1031, 2113
MalI	GATC	6 cut(s)	264, 753, 897, 1512, 1551, 1947
MboI	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
MfeI	CAATTG	1 cut(s)	1893
MflI	RGATCY	1 cut(s)	1549
MhlI	GDGCHC	5 cut(s)	496, 557, 819, 1023, 1372
MluCI	AATT	7 cut(s)	728, 1198, 1241, 1261, 1736, 1747, 1893
MluI	ACGCGT	1 cut(s)	171
Mly113I	GGCGCC	1 cut(s)	152
MlyI	GAGTC	2 cut(s)	924, 1442
MmeI	TCCRAC	2 cut(s)	1758, 1824
MseI	TTAA	4 cut(s)	438, 657, 1382, 1595
MslI	CAYNNNNRTG	4 cut(s)	489, 853, 1353, 2120
MspA1I	CMGCKG	2 cut(s)	7, 641
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	3 cut(s)	284, 758, 1477
MspR9I	CCNGG	6 cut(s)	28, 224, 285, 1047, 1269, 1615
MunI	CAATTG	1 cut(s)	1893
Mva1269I	GAATGC	1 cut(s)	484
MvaI	CCWGG	5 cut(s)	28, 224, 1047, 1269, 1615
MvnI	CGCG	1 cut(s)	173
NarI	GGCGCC	1 cut(s)	152
NciI	CCSGG	1 cut(s)	285
NcoI	CCATGG	2 cut(s)	147, 2127
NdeII	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
NlaIII	CATG	8 cut(s)	151, 409, 858, 1159, 1193, 1546, 1563, 2131
NlaIV	GGNNCC	6 cut(s)	153, 395, 793, 817, 818, 1816
NmuCI	GTSAC	4 cut(s)	316, 688, 1031, 2113
NsbI	TGCGCA	1 cut(s)	1702
NspI	RCATGY	2 cut(s)	858, 1159
PaeR7I	CTCGAG	1 cut(s)	179
PasI	CCCWGGG	1 cut(s)	1268
PciI	ACATGT	1 cut(s)	1155
PciSI	GCTCTTC	2 cut(s)	215, 449
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PctI	GAATGC	1 cut(s)	484
PfeI	GAWTC	1 cut(s)	1398
PflMI	CCANNNNNTGG	1 cut(s)	970
PleI	GAGTC	2 cut(s)	923, 1441
PluTI	GGCGCC	1 cut(s)	155
PpsI	GAGTC	2 cut(s)	923, 1441
PpuMI	RGGWCCY	1 cut(s)	394
PscI	ACATGT	1 cut(s)	1155
PshAI	GACNNNNGTC	1 cut(s)	807
PsiI	TTATAA	3 cut(s)	1086, 1106, 1488
Psp124BI	GAGCTC	1 cut(s)	1023
Psp5II	RGGWCCY	1 cut(s)	394
Psp6I	CCWGG	5 cut(s)	26, 222, 1045, 1267, 1613
PspFI	CCCAGC	2 cut(s)	123, 228
PspGI	CCWGG	5 cut(s)	26, 222, 1045, 1267, 1613
PspN4I	GGNNCC	6 cut(s)	153, 395, 793, 817, 818, 1816
PspOMI	GGGCCC	1 cut(s)	815
PspPI	GGNCC	9 cut(s)	226, 394, 409, 548, 791, 815, 816, 1649, 1866
PspPPI	RGGWCCY	1 cut(s)	394
PstI	CTGCAG	1 cut(s)	1759
PstNI	CAGNNNCTG	1 cut(s)	970
PsuI	RGATCY	1 cut(s)	1549
PvuII	CAGCTG	1 cut(s)	7
RsaI	GTAC	5 cut(s)	1009, 1160, 1392, 2013, 2036
RsaNI	GTAC	5 cut(s)	1008, 1159, 1391, 2012, 2035
RseI	CAYNNNNRTG	4 cut(s)	489, 853, 1353, 2120
SacI	GAGCTC	1 cut(s)	1023
SapI	GCTCTTC	2 cut(s)	215, 449
SaqAI	TTAA	4 cut(s)	438, 657, 1382, 1595
Sau3AI	GATC	6 cut(s)	262, 751, 895, 1510, 1549, 1945
Sau96I	GGNCC	9 cut(s)	226, 394, 409, 548, 791, 815, 816, 1649, 1866
SchI	GAGTC	2 cut(s)	924, 1442
ScrFI	CCNGG	6 cut(s)	28, 224, 285, 1047, 1269, 1615
SduI	GDGCHC	5 cut(s)	496, 557, 819, 1023, 1372
SfaNI	GCATC	7 cut(s)	210, 521, 574, 644, 1357, 1402, 1666
SfcI	CTRYAG	2 cut(s)	1709, 1755
SfiI	GGCCNNNNNGGCC	1 cut(s)	224
SfoI	GGCGCC	1 cut(s)	153
Sfr274I	CTCGAG	1 cut(s)	179
SinI	GGWCC	3 cut(s)	394, 548, 1649
SlaI	CTCGAG	1 cut(s)	179
SmiMI	CAYNNNNRTG	4 cut(s)	489, 853, 1353, 2120
SmlI	CTYRAG	4 cut(s)	179, 437, 515, 707
SmoI	CTYRAG	4 cut(s)	179, 437, 515, 707
Sse9I	AATT	7 cut(s)	728, 1198, 1241, 1261, 1736, 1747, 1893
SsiI	CCGC	6 cut(s)	32, 64, 144, 244, 288, 641
SspDI	GGCGCC	1 cut(s)	151
SspI	AATATT	1 cut(s)	1492
SspMI	CTAG	3 cut(s)	489, 561, 1362
SstI	GAGCTC	1 cut(s)	1023
StyD4I	CCNGG	6 cut(s)	26, 222, 283, 1045, 1267, 1613
StyI	CCWWGG	4 cut(s)	147, 339, 795, 2127
TaaI	ACNGT	6 cut(s)	431, 778, 1002, 1842, 2034, 2119
TaiI	ACGT	1 cut(s)	1888
TaqI	TCGA	5 cut(s)	180, 213, 1509, 1740, 2163
TasI	AATT	7 cut(s)	728, 1198, 1241, 1261, 1736, 1747, 1893
TatI	WGTACW	2 cut(s)	1158, 2034
TauI	GCSGC	3 cut(s)	34, 146, 644
TfiI	GAWTC	1 cut(s)	1398
Tru1I	TTAA	4 cut(s)	438, 657, 1382, 1595
Tru9I	TTAA	4 cut(s)	438, 657, 1382, 1595
TscAI	CASTG	4 cut(s)	1377, 1672, 1686, 1703
TseFI	GTSAC	4 cut(s)	316, 688, 1031, 2113
TseI	GCWGC	8 cut(s)	4, 38, 41, 44, 232, 952, 1193, 1577
Tsp45I	GTSAC	4 cut(s)	316, 688, 1031, 2113
TspDTI	ATGAA	5 cut(s)	477, 1274, 1462, 1464, 1641
TspGWI	ACGGA	4 cut(s)	149, 540, 1408, 1807
TspRI	CASTG	4 cut(s)	1377, 1672, 1686, 1703
Van91I	CCANNNNNTGG	1 cut(s)	970
Vha464I	CTTAAG	1 cut(s)	437
VneI	GTGCAC	2 cut(s)	553, 1368
VpaK11BI	GGWCC	3 cut(s)	394, 548, 1649
XagI	CCTNNNNNAGG	2 cut(s)	420, 880
XapI	RAATTY	2 cut(s)	1241, 1736
XceI	RCATGY	2 cut(s)	858, 1159
XhoI	CTCGAG	1 cut(s)	179
XmiI	GTMKAC	1 cut(s)	1481
XspI	CTAG	3 cut(s)	489, 561, 1362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.