threonine-tRNA ligase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| tRNA_SAD | PF07973 | 170 - 219 | 2.7e-14 | Threonyl and Alanyl tRNA synthetase second additional domain |
| tRNA-synt_2b | PF00587 | 247 - 438 | 3.3e-36 | tRNA synthetase class II core domain (G, H, P, S and T) |
| HGTP_anticodon | PF03129 | 450 - 539 | 1.7e-18 | Anticodon binding domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 720, 740 |
| Acc36I | ACCTGC | 1 cut(s) | 1198 |
| AccB1I | GGYRCC | 2 cut(s) | 707, 1243 |
| AccI | GTMKAC | 2 cut(s) | 516, 945 |
| AciI | CCGC | 1 cut(s) | 554 |
| AclWI | GGATC | 2 cut(s) | 86, 438 |
| AcoI | YGGCCR | 1 cut(s) | 1325 |
| AcsI | RAATTY | 1 cut(s) | 444 |
| AcuI | CTGAAG | 2 cut(s) | 188, 995 |
| AcyI | GRCGYC | 1 cut(s) | 921 |
| AfaI | GTAC | 5 cut(s) | 1001, 1045, 1473, 1496, 1551 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 152, 817, 1369 |
| AgsI | TTSAA | 9 cut(s) | 343, 358, 419, 443, 466, 473, 1575, 1612, 1625 |
| AhlI | ACTAGT | 1 cut(s) | 290 |
| AjiI | CACGTC | 1 cut(s) | 1346 |
| AjnI | CCWGG | 3 cut(s) | 51, 887, 1073 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 987, 1019 |
| Alw21I | GWGCWC | 2 cut(s) | 832, 1311 |
| Alw26I | GTCTC | 1 cut(s) | 1372 |
| Alw44I | GTGCAC | 1 cut(s) | 828 |
| AlwI | GGATC | 2 cut(s) | 86, 438 |
| AoxI | GGCC | 6 cut(s) | 45, 196, 393, 819, 989, 1325 |
| ApaLI | GTGCAC | 1 cut(s) | 828 |
| ApeKI | GCWGC | 2 cut(s) | 61, 1037 |
| ApoI | RAATTY | 1 cut(s) | 444 |
| AseI | ATTAAT | 1 cut(s) | 842 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 173, 1629 |
| AspLEI | GCGC | 4 cut(s) | 30, 75, 224, 414 |
| AspS9I | GGNCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| AsuC2I | CCSGG | 2 cut(s) | 200, 1403 |
| AsuHPI | GGTGA | 6 cut(s) | 139, 167, 341, 1235, 1529, 1555 |
| AvaII | GGWCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| BaeGI | GKGCMC | 1 cut(s) | 832 |
| BanI | GGYRCC | 2 cut(s) | 707, 1243 |
| Bbv12I | GWGCWC | 2 cut(s) | 832, 1311 |
| BbvCI | CCTCAGC | 1 cut(s) | 1032 |
| BbvI | GCAGC | 2 cut(s) | 73, 1049 |
| BccI | CCATC | 3 cut(s) | 56, 1088, 1480 |
| BceAI | ACGGC | 1 cut(s) | 1312 |
| BciT130I | CCWGG | 3 cut(s) | 53, 889, 1075 |
| BciVI | GTATCC | 1 cut(s) | 758 |
| BcnI | CCSGG | 2 cut(s) | 200, 1403 |
| BcoDI | GTCTC | 1 cut(s) | 1372 |
| BcuI | ACTAGT | 1 cut(s) | 290 |
| BfaI | CTAG | 2 cut(s) | 219, 291 |
| BfmI | CTRYAG | 1 cut(s) | 517 |
| BfoI | RGCGCY | 1 cut(s) | 76 |
| BfuAI | ACCTGC | 1 cut(s) | 1198 |
| BfuI | GTATCC | 1 cut(s) | 758 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 53, 1333 |
| BglII | AGATCT | 1 cut(s) | 1410 |
| BisI | GCNGC | 2 cut(s) | 62, 1038 |
| BlpI | GCTNAGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 2 cut(s) | 63, 1039 |
| Bme1390I | CCNGG | 5 cut(s) | 53, 200, 889, 1075, 1403 |
| Bme18I | GGWCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| BmgBI | CACGTC | 1 cut(s) | 1346 |
| BmgT120I | GGNCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| BmiI | GGNNCC | 4 cut(s) | 280, 529, 709, 1245 |
| BmrFI | CCNGG | 5 cut(s) | 53, 200, 889, 1075, 1403 |
| BmsI | GCATC | 5 cut(s) | 39, 307, 862, 1039, 1126 |
| BpmI | CTGGAG | 1 cut(s) | 637 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1032 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 57 |
| BpuEI | CTTGAG | 1 cut(s) | 266 |
| BpuMI | CCSGG | 2 cut(s) | 200, 1403 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1121 |
| BsaHI | GRCGYC | 1 cut(s) | 921 |
| BsaJI | CCNNGG | 4 cut(s) | 531, 888, 1003, 1073 |
| BsaWI | WCCGGW | 1 cut(s) | 810 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 152, 817, 1369 |
| Bse1I | ACTGG | 6 cut(s) | 620, 792, 822, 841, 937, 1540 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1121 |
| BseBI | CCWGG | 3 cut(s) | 53, 889, 1075 |
| BseDI | CCNNGG | 4 cut(s) | 531, 888, 1003, 1073 |
| BseGI | GGATG | 5 cut(s) | 48, 266, 874, 933, 1099 |
| BseJI | GATNNNNATC | 1 cut(s) | 1121 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 152, 817, 1369 |
| BseMII | CTCAG | 5 cut(s) | 48, 384, 1023, 1227, 1496 |
| BseNI | ACTGG | 6 cut(s) | 620, 792, 822, 841, 937, 1540 |
| BseRI | GAGGAG | 1 cut(s) | 1535 |
| BseSI | GKGCMC | 1 cut(s) | 832 |
| BseXI | GCAGC | 2 cut(s) | 73, 1049 |
| BseYI | CCCAGC | 2 cut(s) | 69, 1380 |
| BshFI | GGCC | 6 cut(s) | 47, 198, 395, 821, 991, 1327 |
| BshNI | GGYRCC | 2 cut(s) | 707, 1243 |
| BsiHKAI | GWGCWC | 2 cut(s) | 832, 1311 |
| BsiSI | CCGG | 4 cut(s) | 116, 199, 811, 1402 |
| BslFI | GGGAC | 3 cut(s) | 215, 264, 1418 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 152, 817, 1369 |
| BsmAI | GTCTC | 1 cut(s) | 1372 |
| BsmFI | GGGAC | 3 cut(s) | 215, 264, 1418 |
| BsmI | GAATGC | 3 cut(s) | 214, 414, 604 |
| BsnI | GGCC | 6 cut(s) | 47, 198, 395, 821, 991, 1327 |
| Bsp1286I | GDGCHC | 2 cut(s) | 832, 1311 |
| Bsp143I | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 57 |
| BspACI | CCGC | 1 cut(s) | 554 |
| BspANI | GGCC | 6 cut(s) | 47, 198, 395, 821, 991, 1327 |
| BspCNI | CTCAG | 5 cut(s) | 49, 383, 1024, 1226, 1495 |
| BspLI | GGNNCC | 4 cut(s) | 280, 529, 709, 1245 |
| BspMI | ACCTGC | 1 cut(s) | 1198 |
| BspPI | GGATC | 2 cut(s) | 86, 438 |
| BspQI | GCTCTTC | 1 cut(s) | 44 |
| BspT107I | GGYRCC | 2 cut(s) | 707, 1243 |
| BsrI | ACTGG | 6 cut(s) | 620, 792, 822, 841, 937, 1540 |
| BssECI | CCNNGG | 4 cut(s) | 531, 888, 1003, 1073 |
| BssMI | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| BssNI | GRCGYC | 1 cut(s) | 921 |
| BssT1I | CCWWGG | 2 cut(s) | 531, 1003 |
| Bst2UI | CCWGG | 3 cut(s) | 53, 889, 1075 |
| Bst4CI | ACNGT | 5 cut(s) | 161, 188, 635, 1302, 1579 |
| Bst6I | CTCTTC | 3 cut(s) | 44, 294, 786 |
| BstACI | GRCGYC | 1 cut(s) | 921 |
| BstC8I | GCNNGC | 2 cut(s) | 66, 1195 |
| BstDEI | CTNAG | 7 cut(s) | 57, 370, 925, 1032, 1213, 1482, 1556 |
| BstF5I | GGATG | 5 cut(s) | 48, 266, 874, 933, 1099 |
| BstH2I | RGCGCY | 1 cut(s) | 76 |
| BstHHI | GCGC | 4 cut(s) | 30, 75, 224, 414 |
| BstKTI | GATC | 5 cut(s) | 94, 433, 487, 973, 1413 |
| BstMAI | GTCTC | 1 cut(s) | 1372 |
| BstMBI | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| BstNI | CCWGG | 3 cut(s) | 53, 889, 1075 |
| BstNSI | RCATGY | 1 cut(s) | 594 |
| BstSCI | CCNGG | 5 cut(s) | 51, 198, 887, 1073, 1401 |
| BstSFI | CTRYAG | 1 cut(s) | 517 |
| BstSLI | GKGCMC | 1 cut(s) | 832 |
| BstV1I | GCAGC | 2 cut(s) | 73, 1049 |
| BstX2I | RGATCY | 1 cut(s) | 1410 |
| BstYI | RGATCY | 1 cut(s) | 1410 |
| BsuI | GTATCC | 1 cut(s) | 758 |
| BsuRI | GGCC | 6 cut(s) | 47, 198, 395, 821, 991, 1327 |
| BtrI | CACGTC | 1 cut(s) | 1346 |
| BtsCI | GGATG | 5 cut(s) | 48, 266, 874, 933, 1099 |
| BtsIMutI | CAGTG | 5 cut(s) | 193, 829, 834, 1132, 1163 |
| BveI | ACCTGC | 1 cut(s) | 1198 |
| Cac8I | GCNNGC | 2 cut(s) | 66, 1195 |
| CfoI | GCGC | 4 cut(s) | 30, 75, 224, 414 |
| Cfr13I | GGNCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| CseI | GACGC | 1 cut(s) | 910 |
| Csp6I | GTAC | 5 cut(s) | 1000, 1044, 1472, 1495, 1550 |
| CviAII | CATG | 3 cut(s) | 591, 1020, 1588 |
| CviQI | GTAC | 5 cut(s) | 1000, 1044, 1472, 1495, 1550 |
| DdeI | CTNAG | 7 cut(s) | 57, 370, 925, 1032, 1213, 1482, 1556 |
| DpnI | GATC | 5 cut(s) | 93, 432, 486, 972, 1412 |
| DpnII | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| EaeI | YGGCCR | 1 cut(s) | 1325 |
| Eam1104I | CTCTTC | 3 cut(s) | 44, 294, 786 |
| EarI | CTCTTC | 3 cut(s) | 44, 294, 786 |
| Eco130I | CCWWGG | 2 cut(s) | 531, 1003 |
| Eco32I | GATATC | 1 cut(s) | 1123 |
| Eco47I | GGWCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| Eco57I | CTGAAG | 2 cut(s) | 188, 995 |
| EcoRII | CCWGG | 3 cut(s) | 51, 887, 1073 |
| EcoRV | GATATC | 1 cut(s) | 1123 |
| EcoT14I | CCWWGG | 2 cut(s) | 531, 1003 |
| EcoT22I | ATGCAT | 2 cut(s) | 1054, 1141 |
| ErhI | CCWWGG | 2 cut(s) | 531, 1003 |
| FaeI | CATG | 3 cut(s) | 594, 1023, 1591 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 908, 940 |
| FaqI | GGGAC | 3 cut(s) | 215, 264, 1418 |
| FatI | CATG | 3 cut(s) | 590, 1019, 1587 |
| FblI | GTMKAC | 2 cut(s) | 516, 945 |
| Fnu4HI | GCNGC | 2 cut(s) | 62, 1038 |
| FokI | GGATG | 5 cut(s) | 35, 273, 881, 920, 1106 |
| Fsp4HI | GCNGC | 2 cut(s) | 62, 1038 |
| FspBI | CTAG | 2 cut(s) | 219, 291 |
| GlaI | GCGC | 4 cut(s) | 29, 74, 223, 413 |
| GluI | GCNGC | 2 cut(s) | 62, 1038 |
| GsaI | CCCAGC | 2 cut(s) | 73, 1384 |
| GsuI | CTGGAG | 1 cut(s) | 637 |
| HaeII | RGCGCY | 1 cut(s) | 76 |
| HaeIII | GGCC | 6 cut(s) | 47, 198, 395, 821, 991, 1327 |
| HapII | CCGG | 4 cut(s) | 116, 199, 811, 1402 |
| HgaI | GACGC | 1 cut(s) | 910 |
| HhaI | GCGC | 4 cut(s) | 30, 75, 224, 414 |
| Hin1I | GRCGYC | 1 cut(s) | 921 |
| Hin1II | CATG | 3 cut(s) | 594, 1023, 1591 |
| Hin6I | GCGC | 4 cut(s) | 28, 73, 222, 412 |
| HinP1I | GCGC | 4 cut(s) | 28, 73, 222, 412 |
| HincII | GTYRAC | 1 cut(s) | 1441 |
| HindII | GTYRAC | 1 cut(s) | 1441 |
| HindIII | AAGCTT | 3 cut(s) | 640, 725, 1626 |
| HinfI | GANTC | 7 cut(s) | 368, 462, 513, 745, 858, 893, 1571 |
| HpaI | GTTAAC | 1 cut(s) | 1441 |
| HpaII | CCGG | 4 cut(s) | 116, 199, 811, 1402 |
| HphI | GGTGA | 6 cut(s) | 139, 167, 341, 1235, 1529, 1555 |
| Hpy166II | GTNNAC | 8 cut(s) | 177, 266, 517, 551, 830, 946, 1343, 1441 |
| Hpy188I | TCNGA | 9 cut(s) | 91, 236, 373, 454, 512, 975, 1135, 1372, 1485 |
| Hpy188III | TCNNGA | 3 cut(s) | 100, 671, 853 |
| Hpy8I | GTNNAC | 8 cut(s) | 177, 266, 517, 551, 830, 946, 1343, 1441 |
| HpyAV | CCTTC | 4 cut(s) | 118, 124, 188, 1223 |
| HpyCH4III | ACNGT | 5 cut(s) | 161, 188, 635, 1302, 1579 |
| HpyCH4IV | ACGT | 1 cut(s) | 1345 |
| HpyCH4V | TGCA | 8 cut(s) | 273, 590, 808, 830, 1052, 1100, 1139, 1163 |
| HpyF3I | CTNAG | 7 cut(s) | 57, 370, 925, 1032, 1213, 1482, 1556 |
| HpySE526I | ACGT | 1 cut(s) | 1345 |
| Hsp92I | GRCGYC | 1 cut(s) | 921 |
| Hsp92II | CATG | 3 cut(s) | 594, 1023, 1591 |
| HspAI | GCGC | 4 cut(s) | 28, 73, 222, 412 |
| KspAI | GTTAAC | 1 cut(s) | 1441 |
| Kzo9I | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| LguI | GCTCTTC | 1 cut(s) | 44 |
| LmnI | GCTCC | 2 cut(s) | 533, 1306 |
| Lsp1109I | GCAGC | 2 cut(s) | 73, 1049 |
| LweI | GCATC | 5 cut(s) | 39, 307, 862, 1039, 1126 |
| MaeI | CTAG | 2 cut(s) | 219, 291 |
| MaeII | ACGT | 1 cut(s) | 1345 |
| MaeIII | GTNAC | 2 cut(s) | 155, 894 |
| MalI | GATC | 5 cut(s) | 93, 432, 486, 972, 1412 |
| MboI | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| MboII | GAAGA | 7 cut(s) | 31, 311, 479, 597, 685, 773, 1238 |
| MfeI | CAATTG | 1 cut(s) | 1353 |
| MflI | RGATCY | 1 cut(s) | 1410 |
| MhlI | GDGCHC | 2 cut(s) | 832, 1311 |
| MluCI | AATT | 7 cut(s) | 18, 363, 444, 714, 843, 1353, 1509 |
| MlyI | GAGTC | 3 cut(s) | 362, 522, 902 |
| MmeI | TCCRAC | 1 cut(s) | 1284 |
| Mph1103I | ATGCAT | 2 cut(s) | 1054, 1141 |
| MroXI | GAANNNNTTC | 2 cut(s) | 173, 1629 |
| MseI | TTAA | 6 cut(s) | 390, 842, 915, 1055, 1142, 1440 |
| MslI | CAYNNNNRTG | 2 cut(s) | 589, 813 |
| MspA1I | CMGCKG | 1 cut(s) | 494 |
| MspI | CCGG | 4 cut(s) | 116, 199, 811, 1402 |
| MspR9I | CCNGG | 5 cut(s) | 53, 200, 889, 1075, 1403 |
| MunI | CAATTG | 1 cut(s) | 1353 |
| Mva1269I | GAATGC | 3 cut(s) | 214, 414, 604 |
| MvaI | CCWGG | 3 cut(s) | 53, 889, 1075 |
| NciI | CCSGG | 2 cut(s) | 200, 1403 |
| NdeII | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| NlaIII | CATG | 3 cut(s) | 594, 1023, 1591 |
| NlaIV | GGNNCC | 4 cut(s) | 280, 529, 709, 1245 |
| NmuCI | GTSAC | 2 cut(s) | 155, 894 |
| NsiI | ATGCAT | 2 cut(s) | 1054, 1141 |
| NspI | RCATGY | 1 cut(s) | 594 |
| PciSI | GCTCTTC | 1 cut(s) | 44 |
| PctI | GAATGC | 3 cut(s) | 214, 414, 604 |
| PdmI | GAANNNNTTC | 2 cut(s) | 173, 1629 |
| PfeI | GAWTC | 4 cut(s) | 462, 745, 858, 1571 |
| PfoI | TCCNGGA | 1 cut(s) | 1401 |
| PkrI | GCNGC | 2 cut(s) | 63, 1039 |
| PleI | GAGTC | 3 cut(s) | 362, 521, 901 |
| PpsI | GAGTC | 3 cut(s) | 362, 521, 901 |
| PshBI | ATTAAT | 1 cut(s) | 842 |
| PsiI | TTATAA | 2 cut(s) | 720, 740 |
| Psp6I | CCWGG | 3 cut(s) | 51, 887, 1073 |
| PspFI | CCCAGC | 2 cut(s) | 69, 1380 |
| PspGI | CCWGG | 3 cut(s) | 51, 887, 1073 |
| PspN4I | GGNNCC | 4 cut(s) | 280, 529, 709, 1245 |
| PspPI | GGNCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| PsuI | RGATCY | 1 cut(s) | 1410 |
| PvuII | CAGCTG | 1 cut(s) | 494 |
| RsaI | GTAC | 5 cut(s) | 1001, 1045, 1473, 1496, 1551 |
| RsaNI | GTAC | 5 cut(s) | 1000, 1044, 1472, 1495, 1550 |
| RseI | CAYNNNNRTG | 2 cut(s) | 589, 813 |
| SapI | GCTCTTC | 1 cut(s) | 44 |
| SaqAI | TTAA | 6 cut(s) | 390, 842, 915, 1055, 1142, 1440 |
| SatI | GCNGC | 2 cut(s) | 62, 1038 |
| Sau3AI | GATC | 5 cut(s) | 91, 430, 484, 970, 1410 |
| Sau96I | GGNCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| SchI | GAGTC | 3 cut(s) | 362, 522, 902 |
| ScrFI | CCNGG | 5 cut(s) | 53, 200, 889, 1075, 1403 |
| SduI | GDGCHC | 2 cut(s) | 832, 1311 |
| SfaNI | GCATC | 5 cut(s) | 39, 307, 862, 1039, 1126 |
| SfcI | CTRYAG | 1 cut(s) | 517 |
| SinI | GGWCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 589, 813 |
| SmlI | CTYRAG | 1 cut(s) | 245 |
| SmoI | CTYRAG | 1 cut(s) | 245 |
| SpeI | ACTAGT | 1 cut(s) | 290 |
| Sse9I | AATT | 7 cut(s) | 18, 363, 444, 714, 843, 1353, 1509 |
| SsiI | CCGC | 1 cut(s) | 554 |
| SspI | AATATT | 1 cut(s) | 111 |
| SspMI | CTAG | 2 cut(s) | 219, 291 |
| StyD4I | CCNGG | 5 cut(s) | 51, 198, 887, 1073, 1401 |
| StyI | CCWWGG | 2 cut(s) | 531, 1003 |
| TaaI | ACNGT | 5 cut(s) | 161, 188, 635, 1302, 1579 |
| TaiI | ACGT | 1 cut(s) | 1348 |
| TaqI | TCGA | 3 cut(s) | 42, 784, 1281 |
| TasI | AATT | 7 cut(s) | 18, 363, 444, 714, 843, 1353, 1509 |
| TatI | WGTACW | 1 cut(s) | 1494 |
| TfiI | GAWTC | 4 cut(s) | 462, 745, 858, 1571 |
| Tru1I | TTAA | 6 cut(s) | 390, 842, 915, 1055, 1142, 1440 |
| Tru9I | TTAA | 6 cut(s) | 390, 842, 915, 1055, 1142, 1440 |
| TscAI | CASTG | 5 cut(s) | 193, 829, 841, 1132, 1163 |
| TseFI | GTSAC | 2 cut(s) | 155, 894 |
| TseI | GCWGC | 2 cut(s) | 61, 1037 |
| Tsp45I | GTSAC | 2 cut(s) | 155, 894 |
| TspDTI | ATGAA | 5 cut(s) | 207, 363, 922, 924, 1622 |
| TspRI | CASTG | 5 cut(s) | 193, 829, 841, 1132, 1163 |
| VneI | GTGCAC | 1 cut(s) | 828 |
| VpaK11BI | GGWCC | 5 cut(s) | 278, 551, 1109, 1399, 1455 |
| VspI | ATTAAT | 1 cut(s) | 842 |
| XapI | RAATTY | 1 cut(s) | 444 |
| XceI | RCATGY | 1 cut(s) | 594 |
| XmiI | GTMKAC | 2 cut(s) | 516, 945 |
| XmnI | GAANNNNTTC | 2 cut(s) | 173, 1629 |
| XspI | CTAG | 2 cut(s) | 219, 291 |
| Zsp2I | ATGCAT | 2 cut(s) | 1054, 1141 |