Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

22050704 .. 22056944

Gene Structure 6241 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG103300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGCTGCAATGCTCTAAAACCTGGATATCAACAGCAGCCGCTTTCTCATTTTCTCTTCTCCGCCGTAACTCTCCCTCATTCTCCACTTTGACTCCGCCGCCGTACCGGAAAGACTTCTCTTTCTCCTTCCTACAACCACCGCCAGCCGCCGCCATGGTGCCCCACCCCAAAGACGACGCTTACCTCGATAGCGTAATTCCCAAGCGAATCCAGCTCTTCGAGGCCATCCAGGCTCAGCAGCTCGCCCACCGCCAGTCCCTCCCTTCCGATCCAATCAAGATTACGTTGCCCGACGGGAAGGTGAAGGAGGGGAAGACGTGGGTGACTTCGCCCTTCGACGTTGCGAAGGAGATTTCGAAGAGCTTGGCGTCAAATGCCTTGATTTCTGAGGTCAATGGGGTACTGTGGGACATGAGTAGGCCTTTGGAGGGTGACTGTGAGCTCAAGCTCTTCACATTTGACAATGATGAAGGCAGGGACACTTTCTGGCATTCTAGCGCGCACATTCTCGGACAGGCACTTGAGGTTGAGTACGGATGCAAGTTGTGTATCGGTCCCTGCACCACCAGAGGAGAGGGTTTCTATTATGATGCATTTTATGGTGATTTGGGGTTGAACGAGGATCACTTCAAGCAAATTGAATCAGGAGCAGATAAGGCTGTTAAGGCCAAACAGCCATTTGAGCGCATTGAAGTAACAAGGGATCAGGCACTTGAGATTTTTTCTGATAACAGATTCAAGGTTGAAATCATCAATGATCTTCCTGCTGACAAAACTATCACCGTCTACAGATGTGGCCCCTTGGTTGACTTGTGTCGTGGACCGCACATACCAAACACATCATTTGTCAAAGCATTGGCGTGTTTGAAGGCTTCTTCTGCCTACTGGAGAGGAAATAAAGATCGTGAAAGCTTACAGAGAGTTTATGGAATATCTTATCCTGATAAGAAAAGTTTGCAGTTATATCTCAAGCGTCTGGAAGAAGCAAAAAAATATGATCATAGATTGTTAGGTACAAAGCAGGAGCTGTTCTTTAATCACCCACTTAGTCCAGGAAGTTGGTTCTTCCTGCCCAATGGCACCCGGATTTATAACAAGCTCATGGAGTTTATAAAAAATCAATACAGAGAGAGAGGATACCAAGAGGTTATATCGCCAAACATGTACAATATGCAACTTTGGGAAACATCTGGTCATGCTGCAAATTACAAGGAGAATATGTTTGTCTTTGAGATTGAGAAACAGGAGTTTGGATTGAAGCCAATGAATTGTCCGGGGCATTGTTTAATGTTTCAGCACAGAGTTCGTTCTTATAGAGAGCTTCCTCTTCGACTGGCTGACTTTGGGGTTTTGCACCGGAATGAGGCCAGTGGTGCACTCACTGGATTAACTCGTGTCAGGAGATTCCAGCAGGATGATGCTCATATCTTTTGCAGGGAGTCACAAATAAAAGATGAAGTTACGGGCGTCTTAGACTTCATCCAATATACCTACGATATATTTGGCTTCACTTTTGATCTGAAGCTATCAACAAGGCCAGAAAAGTACCTTGGAGAGATAGAAACATGGGAGAAAGCTGAGTCAGCTCTTACAGAAGCATTAAATGAGTTTGGCAAACCCTGGCAGATAAATGAAGGGGATGGTGCATTTTACGGACCAAAGATAGATATCAGTGTATCTGATGCATTGAACAGGAAGTTTCAGTGTGCAACATTACAGCTTGATTTTCAGCTACCTGCTCGCTTTGAGCTGTATTACTCAGCAGAGGGTGAAGAAGGCAAGAGGGAGGCACCTGTTATGATACACAGAGCCATTTTGGGTTCTGTTGAGCGTATGTTTGCTATACTGTTGGAGCACTACAAGGGGAAATGGCCATTCTGGCTTAGTCCACGTCAGGCAATTGTTTGCCCTGTCTCGGACAAATCCCAGCCCTATGCTCAGCAGGTACGGGACAAGATCTTTCAGGCTGGTTATTTTGTTGATGTTGACACAAGTGATAGGACAATACAGAAAAAGGTACGAGAAGCTCAGATGGCACAGTACAACTACATTCTAGTTGTTGGTGAGGAGGAAGTCAAAACTGGACAGGTGAGCGTACGGGTTAGAGATAAGGCAGATTCCAACGTAATGAGCATGGATAGCCTACTCAAACACTTTAAGGAGGAAGTTGAAGCTTTCCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

727

Amino Acids

83.46

Weight (kDa)

6.42

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	92 - 152	3.5e-17	TGS domain
tRNA_SAD	PF07973	260 - 309	3e-12	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	408 - 620	1.2e-45	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	632 - 721	1.1e-21	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1092, 1112
Acc36I	ACCTGC	2 cut(s)	1744, 1933
AccB1I	GGYRCC	3 cut(s)	157, 1079, 1789
AccI	GTMKAC	1 cut(s)	786
AccII	CGCG	1 cut(s)	500
AciI	CCGC	9 cut(s)	39, 61, 95, 98, 140, 147, 150, 250, 824
AclWI	GGATC	3 cut(s)	263, 630, 711
AcoI	YGGCCR	1 cut(s)	1871
AcuI	CTGAAG	1 cut(s)	1541
AcyI	GRCGYC	2 cut(s)	368, 1467
AfiI	CCNNNNNNNGG	3 cut(s)	428, 1363, 1915
AflIII	ACRYGT	1 cut(s)	1161
AjiI	CACGTC	2 cut(s)	318, 1892
AjnI	CCWGG	4 cut(s)	20, 228, 1051, 1619
AjuI	GAANNNNNNNTTGG	2 cut(s)	1533, 1565
Alw21I	GWGCWC	3 cut(s)	444, 1378, 1857
Alw26I	GTCTC	1 cut(s)	1918
Alw44I	GTGCAC	1 cut(s)	1374
AlwI	GGATC	3 cut(s)	263, 630, 711
AlwNI	CAGNNNCTG	1 cut(s)	1027
AoxI	GGCC	7 cut(s)	222, 419, 666, 796, 1365, 1535, 1871
ApaLI	GTGCAC	1 cut(s)	1374
ApeKI	GCWGC	4 cut(s)	4, 35, 238, 1199
Asp700I	GAANNNNTTC	1 cut(s)	113
AspLEI	GCGC	3 cut(s)	500, 502, 687
AspS9I	GGNCC	4 cut(s)	554, 797, 821, 1655
AsuC2I	CCSGG	2 cut(s)	1084, 1275
AsuHPI	GGTGA	9 cut(s)	313, 334, 443, 614, 772, 1031, 1781, 2075, 2101
AsuII	TTCGAA	1 cut(s)	356
AvaII	GGWCC	3 cut(s)	554, 821, 1655
BaeGI	GKGCMC	2 cut(s)	162, 1378
BalI	TGGCCA	1 cut(s)	1873
BanI	GGYRCC	3 cut(s)	157, 1079, 1789
BanII	GRGCYC	1 cut(s)	444
BauI	CACGAG	1 cut(s)	1392
BbsI	GAAGAC	1 cut(s)	320
Bbv12I	GWGCWC	3 cut(s)	444, 1378, 1857
BbvI	GCAGC	3 cut(s)	47, 250, 1186
BccI	CCATC	3 cut(s)	233, 1634, 2026
BceAI	ACGGC	2 cut(s)	48, 85
BcgI	CGANNNNNNTGC	2 cut(s)	1286, 1320
BciT130I	CCWGG	4 cut(s)	22, 230, 1053, 1621
BciVI	GTATCC	1 cut(s)	1130
BclI	TGATCA	1 cut(s)	997
BcnI	CCSGG	2 cut(s)	1084, 1275
BcoDI	GTCTC	1 cut(s)	1918
BfaI	CTAG	3 cut(s)	495, 2054, 2182
BfmI	CTRYAG	1 cut(s)	787
BfuAI	ACCTGC	2 cut(s)	1744, 1933
BfuI	GTATCC	1 cut(s)	1130
BglI	GCCNNNNNGGC	2 cut(s)	230, 1879
BglII	AGATCT	1 cut(s)	1956
BisI	GCNGC	8 cut(s)	5, 36, 39, 98, 147, 150, 239, 1200
BlpI	GCTNAGC	2 cut(s)	234, 1938
BlsI	GCNGC	8 cut(s)	6, 37, 40, 99, 148, 151, 240, 1201
Bme1390I	CCNGG	6 cut(s)	22, 230, 1053, 1084, 1275, 1621
Bme18I	GGWCC	3 cut(s)	554, 821, 1655
BmgBI	CACGTC	2 cut(s)	318, 1892
BmgT120I	GGNCC	4 cut(s)	554, 797, 821, 1655
BmiI	GGNNCC	5 cut(s)	159, 556, 799, 1081, 1791
BmrFI	CCNGG	6 cut(s)	22, 230, 1053, 1084, 1275, 1621
BmsI	GCATC	4 cut(s)	527, 580, 1408, 1672
BoxI	GACNNNNGTC	1 cut(s)	813
BpiI	GAAGAC	1 cut(s)	320
BpmI	CTGGAG	1 cut(s)	907
Bpu1102I	GCTNAGC	2 cut(s)	234, 1938
Bpu14I	TTCGAA	1 cut(s)	356
BpuEI	CTTGAG	4 cut(s)	428, 542, 734, 953
BpuMI	CCSGG	2 cut(s)	1084, 1275
BsaBI	GATNNNNATC	1 cut(s)	1667
BsaHI	GRCGYC	2 cut(s)	368, 1467
BsaJI	CCNNGG	5 cut(s)	153, 801, 1274, 1549, 1619
BsaWI	WCCGGW	2 cut(s)	105, 1356
BsaXI	ACNNNNNCTCC	4 cut(s)	76, 106, 1205, 1235
Bsc4I	CCNNNNNNNGG	3 cut(s)	428, 1363, 1915
Bse1I	ACTGG	6 cut(s)	253, 890, 1338, 1368, 1387, 2086
Bse3DI	GCAATG	1 cut(s)	14
Bse8I	GATNNNNATC	1 cut(s)	1667
BseBI	CCWGG	4 cut(s)	22, 230, 1053, 1621
BseDI	CCNNGG	5 cut(s)	153, 801, 1274, 1549, 1619
BseGI	GGATG	5 cut(s)	225, 542, 1420, 1479, 1645
BseJI	GATNNNNATC	1 cut(s)	1667
BseLI	CCNNNNNNNGG	3 cut(s)	428, 1363, 1915
BseMI	GCAATG	1 cut(s)	14
BseMII	CTCAG	6 cut(s)	248, 378, 1569, 1773, 1952, 2042
BseNI	ACTGG	6 cut(s)	253, 890, 1338, 1368, 1387, 2086
BsePI	GCGCGC	1 cut(s)	498
BseRI	GAGGAG	2 cut(s)	585, 2081
BseSI	GKGCMC	2 cut(s)	162, 1378
BseXI	GCAGC	3 cut(s)	47, 250, 1186
BseYI	CCCAGC	1 cut(s)	1926
BsgI	GTGCAG	1 cut(s)	544
Bsh1236I	CGCG	1 cut(s)	500
BshFI	GGCC	7 cut(s)	224, 421, 668, 798, 1367, 1537, 1873
BshNI	GGYRCC	3 cut(s)	157, 1079, 1789
BsiHKAI	GWGCWC	3 cut(s)	444, 1378, 1857
BsiSI	CCGG	4 cut(s)	106, 1084, 1274, 1357
BsiWI	CGTACG	1 cut(s)	2095
BslFI	GGGAC	5 cut(s)	241, 422, 491, 540, 1964
BslI	CCNNNNNNNGG	3 cut(s)	428, 1363, 1915
BsmAI	GTCTC	1 cut(s)	1918
BsmFI	GGGAC	5 cut(s)	241, 422, 491, 540, 1964
BsmI	GAATGC	1 cut(s)	490
BsnI	GGCC	7 cut(s)	224, 421, 668, 798, 1367, 1537, 1873
Bsp119I	TTCGAA	1 cut(s)	356
Bsp1286I	GDGCHC	4 cut(s)	162, 444, 1378, 1857
Bsp1407I	TGTACA	1 cut(s)	1164
Bsp143I	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
Bsp1720I	GCTNAGC	2 cut(s)	234, 1938
Bsp19I	CCATGG	1 cut(s)	153
BspACI	CCGC	9 cut(s)	39, 61, 95, 98, 140, 147, 150, 250, 824
BspANI	GGCC	7 cut(s)	224, 421, 668, 798, 1367, 1537, 1873
BspCNI	CTCAG	6 cut(s)	247, 379, 1570, 1772, 1951, 2041
BspFNI	CGCG	1 cut(s)	500
BspLI	GGNNCC	5 cut(s)	159, 556, 799, 1081, 1791
BspMI	ACCTGC	2 cut(s)	1744, 1933
BspPI	GGATC	3 cut(s)	263, 630, 711
BspQI	GCTCTTC	3 cut(s)	221, 353, 455
BspT104I	TTCGAA	1 cut(s)	356
BspT107I	GGYRCC	3 cut(s)	157, 1079, 1789
BsrDI	GCAATG	1 cut(s)	14
BsrGI	TGTACA	1 cut(s)	1164
BsrI	ACTGG	6 cut(s)	253, 890, 1338, 1368, 1387, 2086
BssECI	CCNNGG	5 cut(s)	153, 801, 1274, 1549, 1619
BssHII	GCGCGC	1 cut(s)	498
BssMI	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
BssNI	GRCGYC	2 cut(s)	368, 1467
BssSI	CACGAG	1 cut(s)	1392
BssT1I	CCWWGG	3 cut(s)	153, 801, 1549
Bst2BI	CACGAG	1 cut(s)	1392
Bst2UI	CCWGG	4 cut(s)	22, 230, 1053, 1621
Bst4CI	ACNGT	5 cut(s)	405, 437, 784, 1848, 2040
Bst6I	CTCTTC	5 cut(s)	60, 221, 353, 455, 1332
BstACI	GRCGYC	2 cut(s)	368, 1467
BstAUI	TGTACA	1 cut(s)	1164
BstBI	TTCGAA	1 cut(s)	356
BstC8I	GCNNGC	4 cut(s)	144, 243, 500, 1741
BstDEI	CTNAG	9 cut(s)	234, 387, 1046, 1471, 1578, 1759, 1883, 1938, 2028
BstDSI	CCRYGG	1 cut(s)	153
BstF5I	GGATG	5 cut(s)	225, 542, 1420, 1479, 1645
BstFNI	CGCG	1 cut(s)	500
BstHHI	GCGC	3 cut(s)	500, 502, 687
BstKTI	GATC	8 cut(s)	271, 625, 706, 760, 904, 1000, 1519, 1959
BstMAI	GTCTC	1 cut(s)	1918
BstMBI	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
BstNI	CCWGG	4 cut(s)	22, 230, 1053, 1621
BstNSI	RCATGY	1 cut(s)	1165
BstPAI	GACNNNNGTC	1 cut(s)	813
BstSCI	CCNGG	6 cut(s)	20, 228, 1051, 1082, 1273, 1619
BstSFI	CTRYAG	1 cut(s)	787
BstSLI	GKGCMC	2 cut(s)	162, 1378
BstUI	CGCG	1 cut(s)	500
BstV1I	GCAGC	3 cut(s)	47, 250, 1186
BstV2I	GAAGAC	1 cut(s)	320
BstX2I	RGATCY	1 cut(s)	1956
BstXI	CCANNNNNNTGG	1 cut(s)	1059
BstYI	RGATCY	1 cut(s)	1956
BsuI	GTATCC	1 cut(s)	1130
BsuRI	GGCC	7 cut(s)	224, 421, 668, 798, 1367, 1537, 1873
BtgI	CCRYGG	1 cut(s)	153
BtrI	CACGTC	2 cut(s)	318, 1892
BtsCI	GGATG	5 cut(s)	225, 542, 1420, 1479, 1645
BtsIMutI	CAGTG	4 cut(s)	1375, 1380, 1678, 1709
BveI	ACCTGC	2 cut(s)	1744, 1933
Cac8I	GCNNGC	4 cut(s)	144, 243, 500, 1741
CaiI	CAGNNNCTG	1 cut(s)	1027
CfoI	GCGC	3 cut(s)	500, 502, 687
Cfr13I	GGNCC	4 cut(s)	554, 797, 821, 1655
CseI	GACGC	4 cut(s)	185, 357, 962, 1456
CviAII	CATG	7 cut(s)	154, 412, 1102, 1162, 1196, 1566, 2134
DdeI	CTNAG	9 cut(s)	234, 387, 1046, 1471, 1578, 1759, 1883, 1938, 2028
DpnI	GATC	8 cut(s)	270, 624, 705, 759, 903, 999, 1518, 1958
DpnII	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
EaeI	YGGCCR	1 cut(s)	1871
Eam1104I	CTCTTC	5 cut(s)	60, 221, 353, 455, 1332
EarI	CTCTTC	5 cut(s)	60, 221, 353, 455, 1332
EciI	GGCGGA	2 cut(s)	50, 84
Ecl136II	GAGCTC	1 cut(s)	442
Eco130I	CCWWGG	3 cut(s)	153, 801, 1549
Eco147I	AGGCCT	1 cut(s)	421
Eco24I	GRGCYC	1 cut(s)	444
Eco32I	GATATC	2 cut(s)	27, 1669
Eco47I	GGWCC	3 cut(s)	554, 821, 1655
Eco53kI	GAGCTC	1 cut(s)	442
Eco57I	CTGAAG	1 cut(s)	1541
EcoICRI	GAGCTC	1 cut(s)	442
EcoRII	CCWGG	4 cut(s)	20, 228, 1051, 1619
EcoRV	GATATC	2 cut(s)	27, 1669
EcoT14I	CCWWGG	3 cut(s)	153, 801, 1549
EcoT22I	ATGCAT	2 cut(s)	595, 1687
EcoT38I	GRGCYC	1 cut(s)	444
ErhI	CCWWGG	3 cut(s)	153, 801, 1549
FaeI	CATG	7 cut(s)	157, 415, 1105, 1165, 1199, 1569, 2137
FaqI	GGGAC	5 cut(s)	241, 422, 491, 540, 1964
FatI	CATG	7 cut(s)	153, 411, 1101, 1161, 1195, 1565, 2133
FbaI	TGATCA	1 cut(s)	997
FblI	GTMKAC	1 cut(s)	786
Fnu4HI	GCNGC	8 cut(s)	5, 36, 39, 98, 147, 150, 239, 1200
FokI	GGATG	5 cut(s)	212, 549, 1427, 1466, 1652
FriOI	GRGCYC	1 cut(s)	444
Fsp4HI	GCNGC	8 cut(s)	5, 36, 39, 98, 147, 150, 239, 1200
FspBI	CTAG	3 cut(s)	495, 2054, 2182
GlaI	GCGC	3 cut(s)	499, 501, 686
GluI	GCNGC	8 cut(s)	5, 36, 39, 98, 147, 150, 239, 1200
GsaI	CCCAGC	1 cut(s)	1930
GsuI	CTGGAG	1 cut(s)	907
HaeIII	GGCC	7 cut(s)	224, 421, 668, 798, 1367, 1537, 1873
HapII	CCGG	4 cut(s)	106, 1084, 1274, 1357
HgaI	GACGC	4 cut(s)	185, 357, 962, 1456
HhaI	GCGC	3 cut(s)	500, 502, 687
Hin1I	GRCGYC	2 cut(s)	368, 1467
Hin1II	CATG	7 cut(s)	157, 415, 1105, 1165, 1199, 1569, 2137
Hin6I	GCGC	3 cut(s)	498, 500, 685
HinP1I	GCGC	3 cut(s)	498, 500, 685
HincII	GTYRAC	2 cut(s)	808, 1987
HindII	GTYRAC	2 cut(s)	808, 1987
HindIII	AAGCTT	2 cut(s)	910, 2172
HinfI	GANTC	8 cut(s)	91, 207, 641, 735, 1404, 1439, 1580, 2117
HpaII	CCGG	4 cut(s)	106, 1084, 1274, 1357
HphI	GGTGA	9 cut(s)	313, 334, 443, 614, 772, 1031, 1781, 2075, 2101
Hpy166II	GTNNAC	6 cut(s)	787, 808, 821, 1376, 1889, 1987
Hpy188I	TCNGA	8 cut(s)	268, 388, 512, 727, 1521, 1681, 1918, 2031
Hpy188III	TCNNGA	6 cut(s)	277, 645, 905, 941, 977, 1399
Hpy8I	GTNNAC	6 cut(s)	787, 808, 821, 1376, 1889, 1987
Hpy99I	CGWCG	3 cut(s)	179, 296, 341
HpyCH4III	ACNGT	5 cut(s)	405, 437, 784, 1848, 2040
HpyCH4IV	ACGT	5 cut(s)	284, 317, 339, 1891, 2124
HpyF3I	CTNAG	9 cut(s)	234, 387, 1046, 1471, 1578, 1759, 1883, 1938, 2028
HpySE526I	ACGT	5 cut(s)	284, 317, 339, 1891, 2124
Hsp92I	GRCGYC	2 cut(s)	368, 1467
Hsp92II	CATG	7 cut(s)	157, 415, 1105, 1165, 1199, 1569, 2137
HspAI	GCGC	3 cut(s)	498, 500, 685
Ksp22I	TGATCA	1 cut(s)	997
Kzo9I	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
LguI	GCTCTTC	3 cut(s)	221, 353, 455
LmnI	GCTCC	3 cut(s)	647, 1024, 1852
Lsp1109I	GCAGC	3 cut(s)	47, 250, 1186
LweI	GCATC	4 cut(s)	527, 580, 1408, 1672
MaeI	CTAG	3 cut(s)	495, 2054, 2182
MaeII	ACGT	5 cut(s)	284, 317, 339, 1891, 2124
MaeIII	GTNAC	6 cut(s)	65, 322, 431, 694, 1440, 1459
MalI	GATC	8 cut(s)	270, 624, 705, 759, 903, 999, 1518, 1958
MboI	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
MfeI	CAATTG	1 cut(s)	1899
MflI	RGATCY	1 cut(s)	1956
MhlI	GDGCHC	4 cut(s)	162, 444, 1378, 1857
MlsI	TGGCCA	1 cut(s)	1873
MluCI	AATT	5 cut(s)	195, 636, 1204, 1267, 1899
MluNI	TGGCCA	1 cut(s)	1873
MlyI	GAGTC	3 cut(s)	85, 1448, 1589
MmeI	TCCRAC	2 cut(s)	1830, 2145
Mox20I	TGGCCA	1 cut(s)	1873
Mph1103I	ATGCAT	2 cut(s)	595, 1687
MroXI	GAANNNNTTC	1 cut(s)	113
MscI	TGGCCA	1 cut(s)	1873
MseI	TTAA	6 cut(s)	663, 1035, 1286, 1388, 1601, 2157
MslI	CAYNNNNRTG	2 cut(s)	859, 1359
Msp20I	TGGCCA	1 cut(s)	1873
MspI	CCGG	4 cut(s)	106, 1084, 1274, 1357
MspR9I	CCNGG	6 cut(s)	22, 230, 1053, 1084, 1275, 1621
MunI	CAATTG	1 cut(s)	1899
Mva1269I	GAATGC	1 cut(s)	490
MvaI	CCWGG	4 cut(s)	22, 230, 1053, 1621
MvnI	CGCG	1 cut(s)	500
NciI	CCSGG	2 cut(s)	1084, 1275
NcoI	CCATGG	1 cut(s)	153
NdeII	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
NlaIII	CATG	7 cut(s)	157, 415, 1105, 1165, 1199, 1569, 2137
NlaIV	GGNNCC	5 cut(s)	159, 556, 799, 1081, 1791
NmuCI	GTSAC	3 cut(s)	322, 431, 1440
NsiI	ATGCAT	2 cut(s)	595, 1687
NspI	RCATGY	1 cut(s)	1165
NspV	TTCGAA	1 cut(s)	356
PauI	GCGCGC	1 cut(s)	498
PceI	AGGCCT	1 cut(s)	421
PciI	ACATGT	1 cut(s)	1161
PciSI	GCTCTTC	3 cut(s)	221, 353, 455
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PctI	GAATGC	1 cut(s)	490
PdmI	GAANNNNTTC	1 cut(s)	113
PfeI	GAWTC	5 cut(s)	207, 641, 735, 1404, 2117
Pfl23II	CGTACG	1 cut(s)	2095
PfoI	TCCNGGA	1 cut(s)	1051
PkrI	GCNGC	8 cut(s)	6, 37, 40, 99, 148, 151, 240, 1201
PleI	GAGTC	3 cut(s)	85, 1447, 1588
PpsI	GAGTC	3 cut(s)	85, 1447, 1588
PscI	ACATGT	1 cut(s)	1161
PshAI	GACNNNNGTC	1 cut(s)	813
PsiI	TTATAA	2 cut(s)	1092, 1112
Psp124BI	GAGCTC	1 cut(s)	444
Psp6I	CCWGG	4 cut(s)	20, 228, 1051, 1619
PspFI	CCCAGC	1 cut(s)	1926
PspGI	CCWGG	4 cut(s)	20, 228, 1051, 1619
PspLI	CGTACG	1 cut(s)	2095
PspN4I	GGNNCC	5 cut(s)	159, 556, 799, 1081, 1791
PspPI	GGNCC	4 cut(s)	554, 797, 821, 1655
PstNI	CAGNNNCTG	1 cut(s)	1027
PsuI	RGATCY	1 cut(s)	1956
PteI	GCGCGC	1 cut(s)	498
RseI	CAYNNNNRTG	2 cut(s)	859, 1359
SacI	GAGCTC	1 cut(s)	444
SapI	GCTCTTC	3 cut(s)	221, 353, 455
SaqAI	TTAA	6 cut(s)	663, 1035, 1286, 1388, 1601, 2157
SatI	GCNGC	8 cut(s)	5, 36, 39, 98, 147, 150, 239, 1200
Sau3AI	GATC	8 cut(s)	268, 622, 703, 757, 901, 997, 1516, 1956
Sau96I	GGNCC	4 cut(s)	554, 797, 821, 1655
SchI	GAGTC	3 cut(s)	85, 1448, 1589
ScrFI	CCNGG	6 cut(s)	22, 230, 1053, 1084, 1275, 1621
SduI	GDGCHC	4 cut(s)	162, 444, 1378, 1857
SfaNI	GCATC	4 cut(s)	527, 580, 1408, 1672
SfcI	CTRYAG	1 cut(s)	787
SfuI	TTCGAA	1 cut(s)	356
SinI	GGWCC	3 cut(s)	554, 821, 1655
SmiMI	CAYNNNNRTG	2 cut(s)	859, 1359
SmlI	CTYRAG	4 cut(s)	443, 521, 713, 968
SmoI	CTYRAG	4 cut(s)	443, 521, 713, 968
Sse9I	AATT	5 cut(s)	195, 636, 1204, 1267, 1899
SseBI	AGGCCT	1 cut(s)	421
SsiI	CCGC	9 cut(s)	39, 61, 95, 98, 140, 147, 150, 250, 824
SspMI	CTAG	3 cut(s)	495, 2054, 2182
SstI	GAGCTC	1 cut(s)	444
StuI	AGGCCT	1 cut(s)	421
StyD4I	CCNGG	6 cut(s)	20, 228, 1051, 1082, 1273, 1619
StyI	CCWWGG	3 cut(s)	153, 801, 1549
TaaI	ACNGT	5 cut(s)	405, 437, 784, 1848, 2040
TaiI	ACGT	5 cut(s)	287, 320, 342, 1894, 2127
TaqI	TCGA	5 cut(s)	186, 219, 336, 356, 1330
TaqII	GACCGA	1 cut(s)	542
TasI	AATT	5 cut(s)	195, 636, 1204, 1267, 1899
TatI	WGTACW	2 cut(s)	1164, 2040
TauI	GCSGC	4 cut(s)	41, 100, 149, 152
TfiI	GAWTC	5 cut(s)	207, 641, 735, 1404, 2117
Tru1I	TTAA	6 cut(s)	663, 1035, 1286, 1388, 1601, 2157
Tru9I	TTAA	6 cut(s)	663, 1035, 1286, 1388, 1601, 2157
TscAI	CASTG	4 cut(s)	1375, 1387, 1678, 1709
TseFI	GTSAC	3 cut(s)	322, 431, 1440
TseI	GCWGC	4 cut(s)	4, 35, 238, 1199
Tsp45I	GTSAC	3 cut(s)	322, 431, 1440
TspDTI	ATGAA	5 cut(s)	483, 1280, 1468, 1470, 1647
TspGWI	ACGGA	2 cut(s)	549, 1668
TspRI	CASTG	4 cut(s)	1375, 1387, 1678, 1709
VneI	GTGCAC	1 cut(s)	1374
VpaK11BI	GGWCC	3 cut(s)	554, 821, 1655
XceI	RCATGY	1 cut(s)	1165
XmiI	GTMKAC	1 cut(s)	786
XmnI	GAANNNNTTC	1 cut(s)	113
XspI	CTAG	3 cut(s)	495, 2054, 2182
Zsp2I	ATGCAT	2 cut(s)	595, 1687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.