Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

7072644 .. 7076175

Gene Structure 3532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g11970.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGGAGGTGTTGATCCAAGCTTTCTCAGTTTTGTTCATCATACTTGGTACTTTAGGAGGCTACTTCTTCGGAAGAGCCAGAGGTCGAAAAGATCAAATCACAGTAGACTCCGATCAGCCACAACCTGTATCTTCCGCGGTGGACCAACCCAAAAACGATGTGAAACCGCCACCAGTTGCCGCCATGGTCTCCAAACCTAAAGACGAGGCCGACAAGGAGTCGATTATCTCTAAAAGAAATACACTTTTCAAGTCCAACCAGGCCCAACAAAAAGCCCACATCCTGTCTCTCCCGGCCGAATCCATCAAGATTACATTGCCGGATGGGCAGGTGAAGGATGGGAGGAAGTGGATAACTTCCCCTATGGAAATTGCTGAGGAGATTTCGAAGAGTTTGGCTTCAAAAGCTTTAGTTGCTGAGGTCAATGGGACTCCATGGGACATGGCAAGTCCTTTGGAGTGTGATTGTGAGCTTCAGATATTCACATTTGAAGACAACAAAGGCCGTGACACTTTCTGGCACTCTAGTGCTCATATTCTTGGACAGGCACTTGAGGCTGAGTATGGATGCAAGCTATGTATTGGTCCCTGCACAACAAAACAAGAGGGCTTCTACTATGATGCATATTATGGCAGTTTGGGCTTGAATGATGAGTACTTAAAGAAAATCGAATCAGAGGCAGAGAAGGCTGTCAAGGCAAAACAGTGCTTTGAGCGTATTGAAGTATCAAGGGAACAGGCCCTAGAGATTTTCTCTGATAACAAATTCAAGATTGAGATCATCAATGAGCTTCCTGCGGACAAGGCTATAACTGTATACAGATGTGGCAACTTGGTGGACTTGTGCCGCGGACCTCATATCCCAAGTACATCCTTTGTCAAAGCATTTAGATGCTTGAAGGCTTCTTCAGCATACTGGCGGGGTAATACAGATCGCGAAAGTCTGCAGCGAGTTTATGGAATATCATATCCGGATAAAAAACTTTTGCAGGCATATATCAAGCAGCAGGAAGAAGCAAAGAAGTATGACCATAGGTTGTTGGGAAAATCACAAGATCTCATATTCTGTCACCCATATAGCCCGGGAAGTTGGTTCTTCACCCCCAATGGAGCTCGGATTTATAAGAAACTACTGCAGTTCATGGAAAATGAATATTTAAAAAGGGGATATCAGGAGGTTATAACACCAAACATATTCAAAATGGATTTGTTTGAAAAATCTAAGCATACAGGACACTACAAACAGAACATGTTTCTTTTAGAGATTGAGAAAGAAGAATGTGGCATGAAGCCTATGAACTGTCCTGGTCACTGTGAAGAGTTTCGACATAGGCCTCGTTCTTATAGAGAACTTCCTCTTCGGATGGCTGAATTTGGGGTGCTGCATCGAAATGAGGCCAGTGGTGCGCTAGATGGATTGACTCGTGTCAGGAGATTTATGCAGGATGATGCTCATATATTTTGTAGGGAATCTCAACTGAAAGATGAACTGAAGGGTGTCCTGGACTTCATCAAGTATACCTATGACATATTTGGTCTCTCCTTTGATGTGGAGCTATCAACAAGGCCTGAAAGTTATCATGGAGAACGAGCAACATGGGATAAAGCCGAGGCAATTCTTACAGAAGCACTAAATGAGTTTGGCATGCCTTGGCAGATAAATGAAGGAGATGGAGCATTTTATGGACCAAAGATAGATTTTGGCGTGTGTAATGCTTTGAAGAAAAAATTTCAGTGTGCAACAATACAGCTTGATTTTCAGCTCCCTGATTATGAGCAAGCTGTATTCGGCTCCTTTGAGCGTTTCTTTGGTATACTTTTGGAGCACTACAAGGGGAAATGGCCCTTCTGGCTTAGTCCACGGCAGGCAATTGTTTGCCCCGTCTCAGAGAAATCCGAGGCCTATGCTCTACAGGTTCAGGAACGGATTCGTCGCGCTGGTTATCATGTTGATGTTGACACAAGTGATAGATCCGTGAGTAAAAAGGTGCGAGAAGCTCAGGTGGCGCAATACAACTACATATTAGTTGTTGGTGAGAAGGAAGCTGAAATTGGCCAGGTGAGCTTGCGGGTTCGAGATACAGCAGATGAACATGTCACAGTAATGAGCATCCATGATCTTCTCCAATTCTTCAAAGACAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

715

Amino Acids

81.75

Weight (kDa)

6.25

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	102 - 162	1.8e-17	TGS domain
tRNA_SAD	PF07973	270 - 319	7.3e-13	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	419 - 590	8.3e-33	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	622 - 712	4.1e-19	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1122, 1181
AarI	CACCTGC	1 cut(s)	319
Acc36I	ACCTGC	1 cut(s)	319
AccI	GTMKAC	4 cut(s)	105, 816, 1517, 1813
AccII	CGCG	4 cut(s)	137, 849, 936, 1935
AccIII	TCCGGA	1 cut(s)	970
AciI	CCGC	9 cut(s)	135, 137, 167, 180, 797, 847, 849, 919, 2068
AclWI	GGATC	2 cut(s)	7, 1965
AcoI	YGGCCR	2 cut(s)	294, 2053
AcsI	RAATTY	3 cut(s)	764, 1370, 1727
AcuI	CTGAAG	3 cut(s)	458, 891, 1511
AfaI	GTAC	3 cut(s)	49, 656, 868
AflIII	ACRYGT	2 cut(s)	1248, 2092
AhdI	GACNNNNNGTC	1 cut(s)	217
AjnI	CCWGG	4 cut(s)	258, 1303, 1500, 2055
AjuI	GAANNNNNNNTTGG	2 cut(s)	2034, 2066
AleI	CACNNNNGTG	1 cut(s)	525
Alw21I	GWGCWC	3 cut(s)	532, 1114, 1827
Alw26I	GTCTC	4 cut(s)	193, 291, 1541, 1888
AlwI	GGATC	2 cut(s)	7, 1965
Ama87I	CYCGRG	1 cut(s)	1081
Aor13HI	TCCGGA	1 cut(s)	970
ApeKI	GCWGC	3 cut(s)	946, 1003, 1381
ApoI	RAATTY	3 cut(s)	764, 1370, 1727
Asp700I	GAANNNNTTC	2 cut(s)	1320, 1926
AspLEI	GCGC	3 cut(s)	1408, 1937, 2008
AspS9I	GGNCC	7 cut(s)	142, 262, 584, 739, 851, 1685, 1842
AsuC2I	CCSGG	3 cut(s)	293, 1082, 1083
AsuHPI	GGTGA	5 cut(s)	343, 1061, 1090, 2045, 2071
AsuII	TTCGAA	1 cut(s)	386
AvaI	CYCGRG	1 cut(s)	1081
AvaII	GGWCC	4 cut(s)	142, 584, 851, 1685
BalI	TGGCCA	1 cut(s)	2055
BanII	GRGCYC	1 cut(s)	1114
BauI	CACGAG	1 cut(s)	1422
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	3 cut(s)	532, 1114, 1827
BbvCI	CCTCAGC	2 cut(s)	375, 417
BbvI	GCAGC	3 cut(s)	958, 1015, 1368
BccI	CCATC	6 cut(s)	311, 317, 332, 1357, 1406, 1664
BceAI	ACGGC	2 cut(s)	489, 1877
BcgI	CGANNNNNNTGC	2 cut(s)	1886, 1920
BciT130I	CCWGG	4 cut(s)	260, 1305, 1502, 2057
BcnI	CCSGG	3 cut(s)	293, 1082, 1083
BcoDI	GTCTC	4 cut(s)	193, 291, 1541, 1888
BfaI	CTAG	3 cut(s)	525, 743, 1409
BfmI	CTRYAG	3 cut(s)	944, 1133, 1910
BfuAI	ACCTGC	1 cut(s)	319
BglI	GCCNNNNNGGC	2 cut(s)	325, 1849
BglII	AGATCT	1 cut(s)	1054
BisI	GCNGC	5 cut(s)	180, 847, 947, 1004, 1382
BlsI	GCNGC	5 cut(s)	181, 848, 948, 1005, 1383
BmcAI	AGTACT	1 cut(s)	656
Bme1390I	CCNGG	7 cut(s)	260, 293, 1082, 1083, 1305, 1502, 2057
Bme18I	GGWCC	4 cut(s)	142, 584, 851, 1685
BmeRI	GACNNNNNGTC	1 cut(s)	217
BmeT110I	CYCGRG	1 cut(s)	1081
BmgT120I	GGNCC	7 cut(s)	142, 262, 584, 739, 851, 1685, 1842
BmiI	GGNNCC	2 cut(s)	586, 1792
BmrFI	CCNGG	7 cut(s)	260, 293, 1082, 1083, 1305, 1502, 2057
BmsI	GCATC	6 cut(s)	557, 610, 881, 1393, 1438, 2118
BoxI	GACNNNNGTC	1 cut(s)	1424
BpiI	GAAGAC	1 cut(s)	498
BplI	GAGNNNNNCTC	2 cut(s)	737, 769
Bpu10I	CCTNAGC	3 cut(s)	375, 417, 1998
Bpu14I	TTCGAA	1 cut(s)	386
BpuEI	CTTGAG	1 cut(s)	572
BpuMI	CCSGG	3 cut(s)	293, 1082, 1083
BsaBI	GATNNNNATC	1 cut(s)	776
BsaI	GGTCTC	2 cut(s)	193, 1541
BsaJI	CCNNGG	9 cut(s)	135, 183, 434, 847, 1081, 1608, 1649, 1859, 1896
BsaWI	WCCGGW	1 cut(s)	970
Bse1I	ACTGG	3 cut(s)	173, 920, 1398
Bse3DI	GCAATG	1 cut(s)	314
Bse8I	GATNNNNATC	1 cut(s)	776
BseAI	TCCGGA	1 cut(s)	970
BseBI	CCWGG	4 cut(s)	260, 1305, 1502, 2057
BseDI	CCNNGG	9 cut(s)	135, 183, 434, 847, 1081, 1608, 1649, 1859, 1896
BseGI	GGATG	8 cut(s)	279, 328, 343, 572, 869, 1368, 1450, 2109
BseJI	GATNNNNATC	1 cut(s)	776
BseMI	GCAATG	1 cut(s)	314
BseMII	CTCAG	6 cut(s)	39, 366, 408, 549, 1899, 2012
BseNI	ACTGG	3 cut(s)	173, 920, 1398
BseRI	GAGGAG	1 cut(s)	392
BseX3I	CGGCCG	1 cut(s)	294
BseXI	GCAGC	3 cut(s)	958, 1015, 1368
BsgI	GTGCAG	1 cut(s)	574
Bsh1236I	CGCG	4 cut(s)	137, 849, 936, 1935
Bsh1285I	CGRYCG	1 cut(s)	297
BsiEI	CGRYCG	1 cut(s)	297
BsiHKAI	GWGCWC	3 cut(s)	532, 1114, 1827
BsiHKCI	CYCGRG	1 cut(s)	1081
BsiSI	CCGG	4 cut(s)	293, 320, 971, 1082
BslFI	GGGAC	3 cut(s)	442, 452, 570
BsmAI	GTCTC	4 cut(s)	193, 291, 1541, 1888
BsmBI	CGTCTC	1 cut(s)	1888
BsmFI	GGGAC	3 cut(s)	442, 452, 570
Bso31I	GGTCTC	2 cut(s)	193, 1541
BsoBI	CYCGRG	1 cut(s)	1081
Bsp119I	TTCGAA	1 cut(s)	386
Bsp1286I	GDGCHC	3 cut(s)	532, 1114, 1827
Bsp13I	TCCGGA	1 cut(s)	970
Bsp143I	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
Bsp19I	CCATGG	2 cut(s)	183, 434
Bsp68I	TCGCGA	1 cut(s)	936
BspACI	CCGC	9 cut(s)	135, 137, 167, 180, 797, 847, 849, 919, 2068
BspCNI	CTCAG	6 cut(s)	38, 367, 409, 550, 1898, 2011
BspEI	TCCGGA	1 cut(s)	970
BspFNI	CGCG	4 cut(s)	137, 849, 936, 1935
BspLI	GGNNCC	2 cut(s)	586, 1792
BspMAI	CTGCAG	2 cut(s)	948, 1137
BspMI	ACCTGC	1 cut(s)	319
BspPI	GGATC	2 cut(s)	7, 1965
BspQI	GCTCTTC	1 cut(s)	67
BspT104I	TTCGAA	1 cut(s)	386
BspTNI	GGTCTC	2 cut(s)	193, 1541
BsrDI	GCAATG	1 cut(s)	314
BsrI	ACTGG	3 cut(s)	173, 920, 1398
BssECI	CCNNGG	9 cut(s)	135, 183, 434, 847, 1081, 1608, 1649, 1859, 1896
BssMI	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
BssNAI	GTATAC	3 cut(s)	817, 1518, 1814
BssSI	CACGAG	1 cut(s)	1422
BssT1I	CCWWGG	3 cut(s)	183, 434, 1649
Bst1107I	GTATAC	3 cut(s)	817, 1518, 1814
Bst2BI	CACGAG	1 cut(s)	1422
Bst2UI	CCWGG	4 cut(s)	260, 1305, 1502, 2057
Bst4CI	ACNGT	7 cut(s)	103, 705, 814, 1301, 1313, 2101, 2141
Bst6I	CTCTTC	4 cut(s)	67, 383, 1311, 1362
BstBI	TTCGAA	1 cut(s)	386
BstC8I	GCNNGC	6 cut(s)	572, 990, 1646, 1779, 1866, 2066
BstDEI	CTNAG	8 cut(s)	25, 375, 417, 558, 1221, 1853, 1885, 1998
BstDSI	CCRYGG	5 cut(s)	135, 183, 434, 847, 1859
BstF5I	GGATG	8 cut(s)	279, 328, 343, 572, 869, 1368, 1450, 2109
BstFNI	CGCG	4 cut(s)	137, 849, 936, 1935
BstHHI	GCGC	3 cut(s)	1408, 1937, 2008
BstKTI	GATC	8 cut(s)	15, 94, 115, 780, 934, 1057, 1973, 2119
BstMAI	GTCTC	4 cut(s)	193, 291, 1541, 1888
BstMBI	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
BstMCI	CGRYCG	1 cut(s)	297
BstNI	CCWGG	4 cut(s)	260, 1305, 1502, 2057
BstNSI	RCATGY	3 cut(s)	1252, 1648, 2096
BstPAI	GACNNNNGTC	1 cut(s)	1424
BstSCI	CCNGG	7 cut(s)	258, 291, 1080, 1081, 1303, 1500, 2055
BstSFI	CTRYAG	3 cut(s)	944, 1133, 1910
BstUI	CGCG	4 cut(s)	137, 849, 936, 1935
BstV1I	GCAGC	3 cut(s)	958, 1015, 1368
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	2 cut(s)	1054, 1970
BstYI	RGATCY	2 cut(s)	1054, 1970
BstZ17I	GTATAC	3 cut(s)	817, 1518, 1814
BstZI	CGGCCG	1 cut(s)	294
BtgI	CCRYGG	5 cut(s)	135, 183, 434, 847, 1859
BtsCI	GGATG	8 cut(s)	279, 328, 343, 572, 869, 1368, 1450, 2109
BtsIMutI	CAGTG	4 cut(s)	710, 1309, 1405, 1739
BtuMI	TCGCGA	1 cut(s)	936
BveI	ACCTGC	1 cut(s)	319
Cac8I	GCNNGC	6 cut(s)	572, 990, 1646, 1779, 1866, 2066
CfoI	GCGC	3 cut(s)	1408, 1937, 2008
Cfr13I	GGNCC	7 cut(s)	142, 262, 584, 739, 851, 1685, 1842
Cfr42I	CCGCGG	2 cut(s)	138, 850
Cfr9I	CCCGGG	1 cut(s)	1081
Csp6I	GTAC	3 cut(s)	48, 655, 867
CviQI	GTAC	3 cut(s)	48, 655, 867
DdeI	CTNAG	8 cut(s)	25, 375, 417, 558, 1221, 1853, 1885, 1998
DpnI	GATC	8 cut(s)	14, 93, 114, 779, 933, 1056, 1972, 2118
DpnII	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
DraI	TTTAAA	1 cut(s)	1158
DriI	GACNNNNNGTC	1 cut(s)	217
EaeI	YGGCCR	2 cut(s)	294, 2053
EagI	CGGCCG	1 cut(s)	294
Eam1104I	CTCTTC	4 cut(s)	67, 383, 1311, 1362
Eam1105I	GACNNNNNGTC	1 cut(s)	217
EarI	CTCTTC	4 cut(s)	67, 383, 1311, 1362
Ecl136II	GAGCTC	1 cut(s)	1112
EclXI	CGGCCG	1 cut(s)	294
Eco130I	CCWWGG	3 cut(s)	183, 434, 1649
Eco147I	AGGCCT	3 cut(s)	1333, 1567, 1901
Eco24I	GRGCYC	1 cut(s)	1114
Eco31I	GGTCTC	2 cut(s)	193, 1541
Eco32I	GATATC	1 cut(s)	1169
Eco47I	GGWCC	4 cut(s)	142, 584, 851, 1685
Eco52I	CGGCCG	1 cut(s)	294
Eco53kI	GAGCTC	1 cut(s)	1112
Eco57I	CTGAAG	3 cut(s)	458, 891, 1511
Eco88I	CYCGRG	1 cut(s)	1081
EcoICRI	GAGCTC	1 cut(s)	1112
EcoO109I	RGGNCCY	1 cut(s)	739
EcoRII	CCWGG	4 cut(s)	258, 1303, 1500, 2055
EcoRV	GATATC	1 cut(s)	1169
EcoT14I	CCWWGG	3 cut(s)	183, 434, 1649
EcoT22I	ATGCAT	1 cut(s)	625
EcoT38I	GRGCYC	1 cut(s)	1114
ErhI	CCWWGG	3 cut(s)	183, 434, 1649
Esp3I	CGTCTC	1 cut(s)	1888
FaqI	GGGAC	3 cut(s)	442, 452, 570
FauI	CCCGC	2 cut(s)	912, 2061
FblI	GTMKAC	4 cut(s)	105, 816, 1517, 1813
Fnu4HI	GCNGC	5 cut(s)	180, 847, 947, 1004, 1382
FokI	GGATG	8 cut(s)	266, 335, 350, 579, 856, 1375, 1457, 2096
FriOI	GRGCYC	1 cut(s)	1114
Fsp4HI	GCNGC	5 cut(s)	180, 847, 947, 1004, 1382
FspBI	CTAG	3 cut(s)	525, 743, 1409
GlaI	GCGC	3 cut(s)	1407, 1936, 2007
GluI	GCNGC	5 cut(s)	180, 847, 947, 1004, 1382
HapII	CCGG	4 cut(s)	293, 320, 971, 1082
HhaI	GCGC	3 cut(s)	1408, 1937, 2008
Hin6I	GCGC	3 cut(s)	1406, 1935, 2006
HinP1I	GCGC	3 cut(s)	1406, 1935, 2006
HincII	GTYRAC	1 cut(s)	1957
HindII	GTYRAC	1 cut(s)	1957
HindIII	AAGCTT	2 cut(s)	18, 405
HinfI	GANTC	8 cut(s)	107, 218, 299, 430, 671, 1420, 1469, 1927
HpaII	CCGG	4 cut(s)	293, 320, 971, 1082
HphI	GGTGA	5 cut(s)	343, 1061, 1090, 2045, 2071
Hpy166II	GTNNAC	8 cut(s)	106, 142, 817, 838, 1518, 1814, 1859, 1957
Hpy188I	TCNGA	9 cut(s)	71, 112, 477, 676, 757, 1116, 1362, 1888, 1897
Hpy188III	TCNNGA	8 cut(s)	307, 769, 935, 971, 1172, 1429, 1919, 2075
Hpy8I	GTNNAC	8 cut(s)	106, 142, 817, 838, 1518, 1814, 1859, 1957
Hpy99I	CGWCG	1 cut(s)	1935
HpyAV	CCTTC	7 cut(s)	328, 679, 892, 1486, 1658, 1855, 2032
HpyCH4III	ACNGT	7 cut(s)	103, 705, 814, 1301, 1313, 2101, 2141
HpyCH4V	TGCA	9 cut(s)	570, 591, 623, 946, 988, 1135, 1384, 1441, 1739
HpyF3I	CTNAG	8 cut(s)	25, 375, 417, 558, 1221, 1853, 1885, 1998
HspAI	GCGC	3 cut(s)	1406, 1935, 2006
Kpn2I	TCCGGA	1 cut(s)	970
KspI	CCGCGG	2 cut(s)	138, 850
Kzo9I	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
LguI	GCTCTTC	1 cut(s)	67
LmnI	GCTCC	6 cut(s)	1109, 1552, 1673, 1767, 1796, 1822
Lsp1109I	GCAGC	3 cut(s)	958, 1015, 1368
LweI	GCATC	6 cut(s)	557, 610, 881, 1393, 1438, 2118
MaeI	CTAG	3 cut(s)	525, 743, 1409
MaeIII	GTNAC	4 cut(s)	506, 1067, 1307, 2095
MalI	GATC	8 cut(s)	14, 93, 114, 779, 933, 1056, 1972, 2118
MboI	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
MfeI	CAATTG	1 cut(s)	1869
MflI	RGATCY	2 cut(s)	1054, 1970
MhlI	GDGCHC	3 cut(s)	532, 1114, 1827
MlsI	TGGCCA	1 cut(s)	2055
MluCI	AATT	8 cut(s)	369, 764, 1370, 1614, 1727, 1869, 2049, 2126
MluNI	TGGCCA	1 cut(s)	2055
MlyI	GAGTC	4 cut(s)	101, 227, 424, 1414
MmeI	TCCRAC	1 cut(s)	279
Mox20I	TGGCCA	1 cut(s)	2055
Mph1103I	ATGCAT	1 cut(s)	625
MroI	TCCGGA	1 cut(s)	970
MroXI	GAANNNNTTC	2 cut(s)	1320, 1926
MscI	TGGCCA	1 cut(s)	2055
MseI	TTAA	2 cut(s)	659, 1157
MslI	CAYNNNNRTG	5 cut(s)	525, 889, 1389, 1950, 2102
Msp20I	TGGCCA	1 cut(s)	2055
MspA1I	CMGCKG	2 cut(s)	137, 849
MspI	CCGG	4 cut(s)	293, 320, 971, 1082
MspR9I	CCNGG	7 cut(s)	260, 293, 1082, 1083, 1305, 1502, 2057
MunI	CAATTG	1 cut(s)	1869
MvaI	CCWGG	4 cut(s)	260, 1305, 1502, 2057
MvnI	CGCG	4 cut(s)	137, 849, 936, 1935
NciI	CCSGG	3 cut(s)	293, 1082, 1083
NcoI	CCATGG	2 cut(s)	183, 434
NdeII	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
NlaIV	GGNNCC	2 cut(s)	586, 1792
NmeAIII	GCCGAG	1 cut(s)	1633
NmuCI	GTSAC	4 cut(s)	506, 1067, 1307, 2095
NruI	TCGCGA	1 cut(s)	936
NsiI	ATGCAT	1 cut(s)	625
NspI	RCATGY	3 cut(s)	1252, 1648, 2096
NspV	TTCGAA	1 cut(s)	386
OliI	CACNNNNGTG	1 cut(s)	525
PaeI	GCATGC	1 cut(s)	1648
PaqCI	CACCTGC	1 cut(s)	319
PceI	AGGCCT	3 cut(s)	1333, 1567, 1901
PciI	ACATGT	2 cut(s)	1248, 2092
PciSI	GCTCTTC	1 cut(s)	67
PdmI	GAANNNNTTC	2 cut(s)	1320, 1926
PfeI	GAWTC	4 cut(s)	299, 671, 1469, 1927
PfoI	TCCNGGA	1 cut(s)	1500
PkrI	GCNGC	5 cut(s)	181, 848, 948, 1005, 1383
PleI	GAGTC	4 cut(s)	101, 226, 424, 1414
PpsI	GAGTC	4 cut(s)	101, 226, 424, 1414
PscI	ACATGT	2 cut(s)	1248, 2092
PshAI	GACNNNNGTC	1 cut(s)	1424
PsiI	TTATAA	2 cut(s)	1122, 1181
Psp124BI	GAGCTC	1 cut(s)	1114
Psp6I	CCWGG	4 cut(s)	258, 1303, 1500, 2055
PspGI	CCWGG	4 cut(s)	258, 1303, 1500, 2055
PspN4I	GGNNCC	2 cut(s)	586, 1792
PspPI	GGNCC	7 cut(s)	142, 262, 584, 739, 851, 1685, 1842
PstI	CTGCAG	2 cut(s)	948, 1137
PsuI	RGATCY	2 cut(s)	1054, 1970
RruI	TCGCGA	1 cut(s)	936
RsaI	GTAC	3 cut(s)	49, 656, 868
RsaNI	GTAC	3 cut(s)	48, 655, 867
RseI	CAYNNNNRTG	5 cut(s)	525, 889, 1389, 1950, 2102
SacI	GAGCTC	1 cut(s)	1114
SacII	CCGCGG	2 cut(s)	138, 850
SapI	GCTCTTC	1 cut(s)	67
SaqAI	TTAA	2 cut(s)	659, 1157
SatI	GCNGC	5 cut(s)	180, 847, 947, 1004, 1382
Sau3AI	GATC	8 cut(s)	12, 91, 112, 777, 931, 1054, 1970, 2116
Sau96I	GGNCC	7 cut(s)	142, 262, 584, 739, 851, 1685, 1842
ScaI	AGTACT	1 cut(s)	656
SchI	GAGTC	4 cut(s)	101, 227, 424, 1414
ScrFI	CCNGG	7 cut(s)	260, 293, 1082, 1083, 1305, 1502, 2057
SduI	GDGCHC	3 cut(s)	532, 1114, 1827
SfaNI	GCATC	6 cut(s)	557, 610, 881, 1393, 1438, 2118
SfcI	CTRYAG	3 cut(s)	944, 1133, 1910
Sfr303I	CCGCGG	2 cut(s)	138, 850
SfuI	TTCGAA	1 cut(s)	386
SgrBI	CCGCGG	2 cut(s)	138, 850
SinI	GGWCC	4 cut(s)	142, 584, 851, 1685
SmaI	CCCGGG	1 cut(s)	1083
SmiMI	CAYNNNNRTG	5 cut(s)	525, 889, 1389, 1950, 2102
SmlI	CTYRAG	1 cut(s)	551
SmoI	CTYRAG	1 cut(s)	551
SphI	GCATGC	1 cut(s)	1648
Sse9I	AATT	8 cut(s)	369, 764, 1370, 1614, 1727, 1869, 2049, 2126
SseBI	AGGCCT	3 cut(s)	1333, 1567, 1901
SsiI	CCGC	9 cut(s)	135, 137, 167, 180, 797, 847, 849, 919, 2068
SspI	AATATT	1 cut(s)	1154
SspMI	CTAG	3 cut(s)	525, 743, 1409
SstI	GAGCTC	1 cut(s)	1114
StuI	AGGCCT	3 cut(s)	1333, 1567, 1901
StyD4I	CCNGG	7 cut(s)	258, 291, 1080, 1081, 1303, 1500, 2055
StyI	CCWWGG	3 cut(s)	183, 434, 1649
TaaI	ACNGT	7 cut(s)	103, 705, 814, 1301, 1313, 2101, 2141
TaqI	TCGA	7 cut(s)	85, 221, 386, 669, 1324, 1387, 2074
TasI	AATT	8 cut(s)	369, 764, 1370, 1614, 1727, 1869, 2049, 2126
TatI	WGTACW	2 cut(s)	654, 866
TauI	GCSGC	2 cut(s)	182, 849
TfiI	GAWTC	4 cut(s)	299, 671, 1469, 1927
Tru1I	TTAA	2 cut(s)	659, 1157
Tru9I	TTAA	2 cut(s)	659, 1157
TscAI	CASTG	4 cut(s)	710, 1316, 1405, 1739
TseFI	GTSAC	4 cut(s)	506, 1067, 1307, 2095
TseI	GCWGC	3 cut(s)	946, 1003, 1381
Tsp45I	GTSAC	4 cut(s)	506, 1067, 1307, 2095
TspDTI	ATGAA	9 cut(s)	25, 1129, 1164, 1301, 1310, 1498, 1500, 1677, 2103
TspGWI	ACGGA	2 cut(s)	1939, 1963
TspMI	CCCGGG	1 cut(s)	1081
TspRI	CASTG	4 cut(s)	710, 1316, 1405, 1739
VpaK11BI	GGWCC	4 cut(s)	142, 584, 851, 1685
XapI	RAATTY	3 cut(s)	764, 1370, 1727
XceI	RCATGY	3 cut(s)	1252, 1648, 2096
XmaI	CCCGGG	1 cut(s)	1081
XmiI	GTMKAC	4 cut(s)	105, 816, 1517, 1813
XmnI	GAANNNNTTC	2 cut(s)	1320, 1926
XspI	CTAG	3 cut(s)	525, 743, 1409
ZrmI	AGTACT	1 cut(s)	656
Zsp2I	ATGCAT	1 cut(s)	625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.