threonine--tRNA ligase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TGS | PF02824 | 75 - 135 | 6.5e-17 | TGS domain |
| tRNA_SAD | PF07973 | 243 - 292 | 7.6e-15 | Threonyl and Alanyl tRNA synthetase second additional domain |
| tRNA-synt_2b | PF00587 | 391 - 602 | 3.2e-47 | tRNA synthetase class II core domain (G, H, P, S and T) |
| HGTP_anticodon | PF03129 | 614 - 703 | 9.5e-22 | Anticodon binding domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 584 |
| Acc16I | TGCGCA | 1 cut(s) | 450 |
| Acc36I | ACCTGC | 1 cut(s) | 584 |
| AccI | GTMKAC | 2 cut(s) | 735, 1735 |
| AccII | CGCG | 1 cut(s) | 1837 |
| AccIII | TCCGGA | 1 cut(s) | 1139 |
| AciI | CCGC | 5 cut(s) | 138, 773, 1306, 1532, 1835 |
| AclWI | GGATC | 5 cut(s) | 169, 212, 660, 1542, 1774 |
| AcoI | YGGCCR | 1 cut(s) | 1284 |
| AcsI | RAATTY | 4 cut(s) | 404, 1646, 2099, 2120 |
| AcuI | CTGAAG | 2 cut(s) | 926, 1548 |
| AdeI | CACNNNGTG | 2 cut(s) | 1331, 2071 |
| AfaI | GTAC | 6 cut(s) | 88, 1115, 1384, 1442, 1496, 1988 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 377, 754, 970, 1249, 1312 |
| AflII | CTTAAG | 1 cut(s) | 200 |
| AflIII | ACRYGT | 1 cut(s) | 1437 |
| AjnI | CCWGG | 2 cut(s) | 1000, 1222 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 356, 388 |
| Alw21I | GWGCWC | 1 cut(s) | 1327 |
| Alw26I | GTCTC | 5 cut(s) | 60, 210, 266, 1273, 2046 |
| AlwI | GGATC | 5 cut(s) | 169, 212, 660, 1542, 1774 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 599, 1628, 2021 |
| Ama87I | CYCGRG | 3 cut(s) | 191, 470, 662 |
| Aor13HI | TCCGGA | 1 cut(s) | 1139 |
| ApeKI | GCWGC | 8 cut(s) | 104, 293, 599, 608, 901, 1709, 2021, 2060 |
| ApoI | RAATTY | 4 cut(s) | 404, 1646, 2099, 2120 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 803, 1890 |
| AspLEI | GCGC | 1 cut(s) | 451 |
| AspS9I | GGNCC | 8 cut(s) | 503, 559, 746, 770, 819, 961, 1604, 1818 |
| AsuHPI | GGTGA | 5 cut(s) | 392, 563, 854, 2021, 2047 |
| AvaI | CYCGRG | 3 cut(s) | 191, 470, 662 |
| AvaII | GGWCC | 3 cut(s) | 503, 770, 1604 |
| AxyI | CCTNAGG | 1 cut(s) | 117 |
| BanII | GRGCYC | 1 cut(s) | 1206 |
| BauI | CACGAG | 2 cut(s) | 1032, 1341 |
| BbsI | GAAGAC | 1 cut(s) | 69 |
| Bbv12I | GWGCWC | 1 cut(s) | 1327 |
| BbvCI | CCTCAGC | 1 cut(s) | 1713 |
| BbvI | GCAGC | 8 cut(s) | 116, 280, 586, 595, 913, 1721, 2008, 2072 |
| BccI | CCATC | 5 cut(s) | 236, 277, 533, 1045, 1583 |
| BceAI | ACGGC | 3 cut(s) | 33, 125, 1742 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 578, 612, 1235, 1269 |
| BciT130I | CCWGG | 2 cut(s) | 1002, 1224 |
| BclI | TGATCA | 1 cut(s) | 892 |
| BcoDI | GTCTC | 5 cut(s) | 60, 210, 266, 1273, 2046 |
| BfaI | CTAG | 3 cut(s) | 444, 582, 926 |
| BfmI | CTRYAG | 4 cut(s) | 714, 736, 1548, 2025 |
| BfrI | CTTAAG | 1 cut(s) | 200 |
| BfuAI | ACCTGC | 1 cut(s) | 584 |
| BisI | GCNGC | 8 cut(s) | 105, 294, 600, 609, 902, 1710, 2022, 2061 |
| BlpI | GCTNAGC | 1 cut(s) | 1974 |
| BlsI | GCNGC | 8 cut(s) | 106, 295, 601, 610, 903, 1711, 2023, 2062 |
| Bme1390I | CCNGG | 2 cut(s) | 1002, 1224 |
| Bme18I | GGWCC | 3 cut(s) | 503, 770, 1604 |
| BmeT110I | CYCGRG | 3 cut(s) | 191, 470, 662 |
| BmgT120I | GGNCC | 8 cut(s) | 503, 559, 746, 770, 819, 961, 1604, 1818 |
| BmiI | GGNNCC | 1 cut(s) | 1355 |
| BmrFI | CCNGG | 2 cut(s) | 1002, 1224 |
| BmsI | GCATC | 5 cut(s) | 594, 1357, 1621, 1900, 1918 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1343 |
| BpiI | GAAGAC | 1 cut(s) | 69 |
| Bpu10I | CCTNAGC | 2 cut(s) | 612, 1713 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1974 |
| BpuEI | CTTGAG | 1 cut(s) | 413 |
| BsaAI | YACGTR | 1 cut(s) | 1438 |
| BsaBI | GATNNNNATC | 2 cut(s) | 287, 1616 |
| BsaJI | CCNNGG | 3 cut(s) | 1000, 1001, 1498 |
| BsaWI | WCCGGW | 1 cut(s) | 1139 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 470, 500 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 377, 754, 970, 1249, 1312 |
| Bse1I | ACTGG | 4 cut(s) | 839, 1079, 1287, 1651 |
| Bse21I | CCTNAGG | 1 cut(s) | 117 |
| Bse3DI | GCAATG | 3 cut(s) | 62, 1315, 1641 |
| Bse8I | GATNNNNATC | 2 cut(s) | 287, 1616 |
| BseAI | TCCGGA | 1 cut(s) | 1139 |
| BseBI | CCWGG | 2 cut(s) | 1002, 1224 |
| BseDI | CCNNGG | 3 cut(s) | 1000, 1001, 1498 |
| BseGI | GGATG | 6 cut(s) | 112, 127, 144, 1136, 1369, 1723 |
| BseJI | GATNNNNATC | 2 cut(s) | 287, 1616 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 377, 754, 970, 1249, 1312 |
| BseMI | GCAATG | 3 cut(s) | 62, 1315, 1641 |
| BseMII | CTCAG | 8 cut(s) | 311, 554, 666, 1009, 1704, 1886, 1988, 1997 |
| BseNI | ACTGG | 4 cut(s) | 839, 1079, 1287, 1651 |
| BseRI | GAGGAG | 1 cut(s) | 534 |
| BseXI | GCAGC | 8 cut(s) | 116, 280, 586, 595, 913, 1721, 2008, 2072 |
| BseYI | CCCAGC | 1 cut(s) | 1913 |
| BsgI | GTGCAG | 1 cut(s) | 1430 |
| Bsh1236I | CGCG | 1 cut(s) | 1837 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1327 |
| BsiHKCI | CYCGRG | 3 cut(s) | 191, 470, 662 |
| BsiSI | CCGG | 1 cut(s) | 1140 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 377, 754, 970, 1249, 1312 |
| BsmAI | GTCTC | 5 cut(s) | 60, 210, 266, 1273, 2046 |
| BsmBI | CGTCTC | 1 cut(s) | 60 |
| BsmI | GAATGC | 1 cut(s) | 439 |
| BsoBI | CYCGRG | 3 cut(s) | 191, 470, 662 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1206, 1327 |
| Bsp13I | TCCGGA | 1 cut(s) | 1139 |
| Bsp1407I | TGTACA | 3 cut(s) | 1113, 1382, 1440 |
| Bsp143I | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1974 |
| BspACI | CCGC | 5 cut(s) | 138, 773, 1306, 1532, 1835 |
| BspCNI | CTCAG | 8 cut(s) | 310, 555, 667, 1008, 1705, 1885, 1987, 1996 |
| BspEI | TCCGGA | 1 cut(s) | 1139 |
| BspFNI | CGCG | 1 cut(s) | 1837 |
| BspLI | GGNNCC | 1 cut(s) | 1355 |
| BspMAI | CTGCAG | 1 cut(s) | 718 |
| BspMI | ACCTGC | 1 cut(s) | 584 |
| BspPI | GGATC | 5 cut(s) | 169, 212, 660, 1542, 1774 |
| BspQI | GCTCTTC | 1 cut(s) | 2069 |
| BspTI | CTTAAG | 1 cut(s) | 200 |
| BsrDI | GCAATG | 3 cut(s) | 62, 1315, 1641 |
| BsrGI | TGTACA | 3 cut(s) | 1113, 1382, 1440 |
| BsrI | ACTGG | 4 cut(s) | 839, 1079, 1287, 1651 |
| BssECI | CCNNGG | 3 cut(s) | 1000, 1001, 1498 |
| BssMI | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| BssSI | CACGAG | 2 cut(s) | 1032, 1341 |
| BssT1I | CCWWGG | 1 cut(s) | 1498 |
| Bst2BI | CACGAG | 2 cut(s) | 1032, 1341 |
| Bst2UI | CCWGG | 2 cut(s) | 1002, 1224 |
| Bst4CI | ACNGT | 5 cut(s) | 91, 733, 854, 1353, 1624 |
| Bst6I | CTCTTC | 3 cut(s) | 213, 983, 2069 |
| BstAFI | CTTAAG | 1 cut(s) | 200 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 599, 608 |
| BstAUI | TGTACA | 3 cut(s) | 1113, 1382, 1440 |
| BstBAI | YACGTR | 1 cut(s) | 1438 |
| BstC8I | GCNNGC | 4 cut(s) | 22, 714, 863, 1980 |
| BstF5I | GGATG | 6 cut(s) | 112, 127, 144, 1136, 1369, 1723 |
| BstFNI | CGCG | 1 cut(s) | 1837 |
| BstHHI | GCGC | 1 cut(s) | 451 |
| BstKTI | GATC | 7 cut(s) | 177, 220, 225, 655, 895, 1537, 1769 |
| BstMAI | GTCTC | 5 cut(s) | 60, 210, 266, 1273, 2046 |
| BstMBI | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| BstNI | CCWGG | 2 cut(s) | 1002, 1224 |
| BstNSI | RCATGY | 1 cut(s) | 1148 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1343 |
| BstSCI | CCNGG | 2 cut(s) | 1000, 1222 |
| BstSFI | CTRYAG | 4 cut(s) | 714, 736, 1548, 2025 |
| BstUI | CGCG | 1 cut(s) | 1837 |
| BstV1I | GCAGC | 8 cut(s) | 116, 280, 586, 595, 913, 1721, 2008, 2072 |
| BstV2I | GAAGAC | 1 cut(s) | 69 |
| BstX2I | RGATCY | 3 cut(s) | 174, 1534, 1766 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1795 |
| BstYI | RGATCY | 3 cut(s) | 174, 1534, 1766 |
| Bsu36I | CCTNAGG | 1 cut(s) | 117 |
| BtsCI | GGATG | 6 cut(s) | 112, 127, 144, 1136, 1369, 1723 |
| BtsI | GCAGTG | 1 cut(s) | 1228 |
| BtsIMutI | CAGTG | 2 cut(s) | 1228, 1658 |
| BveI | ACCTGC | 1 cut(s) | 584 |
| Cac8I | GCNNGC | 4 cut(s) | 22, 714, 863, 1980 |
| CaiI | CAGNNNCTG | 3 cut(s) | 599, 1628, 2021 |
| CfoI | GCGC | 1 cut(s) | 451 |
| Cfr13I | GGNCC | 8 cut(s) | 503, 559, 746, 770, 819, 961, 1604, 1818 |
| Csp6I | GTAC | 6 cut(s) | 87, 1114, 1383, 1441, 1495, 1987 |
| CviAII | CATG | 5 cut(s) | 507, 721, 1120, 1145, 1515 |
| CviQI | GTAC | 6 cut(s) | 87, 1114, 1383, 1441, 1495, 1987 |
| DpnI | GATC | 7 cut(s) | 176, 219, 224, 654, 894, 1536, 1768 |
| DpnII | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| DraIII | CACNNNGTG | 2 cut(s) | 1331, 2071 |
| EaeI | YGGCCR | 1 cut(s) | 1284 |
| Eam1104I | CTCTTC | 3 cut(s) | 213, 983, 2069 |
| EarI | CTCTTC | 3 cut(s) | 213, 983, 2069 |
| EciI | GGCGGA | 1 cut(s) | 127 |
| Eco130I | CCWWGG | 1 cut(s) | 1498 |
| Eco24I | GRGCYC | 1 cut(s) | 1206 |
| Eco32I | GATATC | 2 cut(s) | 289, 882 |
| Eco47I | GGWCC | 3 cut(s) | 503, 770, 1604 |
| Eco57I | CTGAAG | 2 cut(s) | 926, 1548 |
| Eco81I | CCTNAGG | 1 cut(s) | 117 |
| Eco88I | CYCGRG | 3 cut(s) | 191, 470, 662 |
| EcoO109I | RGGNCCY | 1 cut(s) | 819 |
| EcoRI | GAATTC | 1 cut(s) | 2099 |
| EcoRII | CCWGG | 2 cut(s) | 1000, 1222 |
| EcoRV | GATATC | 2 cut(s) | 289, 882 |
| EcoT14I | CCWWGG | 1 cut(s) | 1498 |
| EcoT38I | GRGCYC | 1 cut(s) | 1206 |
| ErhI | CCWWGG | 1 cut(s) | 1498 |
| Esp3I | CGTCTC | 1 cut(s) | 60 |
| FaeI | CATG | 5 cut(s) | 510, 724, 1123, 1148, 1518 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1962, 1994 |
| FatI | CATG | 5 cut(s) | 506, 720, 1119, 1144, 1514 |
| FbaI | TGATCA | 1 cut(s) | 892 |
| FblI | GTMKAC | 2 cut(s) | 735, 1735 |
| Fnu4HI | GCNGC | 8 cut(s) | 105, 294, 600, 609, 902, 1710, 2022, 2061 |
| FokI | GGATG | 6 cut(s) | 99, 131, 134, 1123, 1376, 1730 |
| FriOI | GRGCYC | 1 cut(s) | 1206 |
| Fsp4HI | GCNGC | 8 cut(s) | 105, 294, 600, 609, 902, 1710, 2022, 2061 |
| FspAI | RTGCGCAY | 1 cut(s) | 450 |
| FspBI | CTAG | 3 cut(s) | 444, 582, 926 |
| FspI | TGCGCA | 1 cut(s) | 450 |
| GlaI | GCGC | 1 cut(s) | 450 |
| GluI | GCNGC | 8 cut(s) | 105, 294, 600, 609, 902, 1710, 2022, 2061 |
| GsaI | CCCAGC | 1 cut(s) | 1917 |
| HapII | CCGG | 1 cut(s) | 1140 |
| HhaI | GCGC | 1 cut(s) | 451 |
| Hin1II | CATG | 5 cut(s) | 510, 724, 1123, 1148, 1518 |
| Hin6I | GCGC | 1 cut(s) | 449 |
| HinP1I | GCGC | 1 cut(s) | 449 |
| HindIII | AAGCTT | 3 cut(s) | 161, 389, 1554 |
| HinfI | GANTC | 4 cut(s) | 184, 196, 1339, 1687 |
| HpaII | CCGG | 1 cut(s) | 1140 |
| HphI | GGTGA | 5 cut(s) | 392, 563, 854, 2021, 2047 |
| Hpy166II | GTNNAC | 6 cut(s) | 736, 770, 1173, 1441, 1458, 1736 |
| Hpy188I | TCNGA | 9 cut(s) | 222, 282, 300, 676, 897, 1349, 1630, 1686, 1864 |
| Hpy188III | TCNNGA | 8 cut(s) | 193, 425, 470, 664, 890, 926, 1140, 1825 |
| Hpy8I | GTNNAC | 6 cut(s) | 736, 770, 1173, 1441, 1458, 1736 |
| Hpy99I | CGWCG | 2 cut(s) | 36, 1353 |
| HpyAV | CCTTC | 8 cut(s) | 17, 251, 371, 643, 832, 1028, 1577, 1831 |
| HpyCH4III | ACNGT | 5 cut(s) | 91, 733, 854, 1353, 1624 |
| HpyCH4IV | ACGT | 5 cut(s) | 154, 233, 1175, 1437, 1777 |
| HpySE526I | ACGT | 5 cut(s) | 154, 233, 1175, 1437, 1777 |
| Hsp92II | CATG | 5 cut(s) | 510, 724, 1123, 1148, 1518 |
| HspAI | GCGC | 1 cut(s) | 449 |
| Kpn2I | TCCGGA | 1 cut(s) | 1139 |
| Ksp22I | TGATCA | 1 cut(s) | 892 |
| Kzo9I | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| LguI | GCTCTTC | 1 cut(s) | 2069 |
| LmnI | GCTCC | 3 cut(s) | 9, 1322, 1798 |
| Lsp1109I | GCAGC | 8 cut(s) | 116, 280, 586, 595, 913, 1721, 2008, 2072 |
| LweI | GCATC | 5 cut(s) | 594, 1357, 1621, 1900, 1918 |
| MaeI | CTAG | 3 cut(s) | 444, 582, 926 |
| MaeII | ACGT | 5 cut(s) | 154, 233, 1175, 1437, 1777 |
| MaeIII | GTNAC | 9 cut(s) | 229, 234, 566, 643, 842, 1085, 1226, 1269, 1299 |
| MalI | GATC | 7 cut(s) | 176, 219, 224, 654, 894, 1536, 1768 |
| MboI | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| MflI | RGATCY | 3 cut(s) | 174, 1534, 1766 |
| MhlI | GDGCHC | 2 cut(s) | 1206, 1327 |
| MlyI | GAGTC | 1 cut(s) | 1333 |
| MmeI | TCCRAC | 4 cut(s) | 119, 1372, 1401, 1776 |
| MnlI | CCTC | 9 cut(s) | 512, 517, 810, 832, 1087, 1306, 1476, 1708, 2077 |
| MroI | TCCGGA | 1 cut(s) | 1139 |
| MroXI | GAANNNNTTC | 2 cut(s) | 803, 1890 |
| MseI | TTAA | 7 cut(s) | 201, 687, 1062, 1179, 1398, 1905, 1937 |
| MslI | CAYNNNNRTG | 2 cut(s) | 444, 1308 |
| MspA1I | CMGCKG | 3 cut(s) | 493, 904, 1712 |
| MspCI | CTTAAG | 1 cut(s) | 200 |
| MspI | CCGG | 1 cut(s) | 1140 |
| MspR9I | CCNGG | 2 cut(s) | 1002, 1224 |
| Mva1269I | GAATGC | 1 cut(s) | 439 |
| MvaI | CCWGG | 2 cut(s) | 1002, 1224 |
| MvnI | CGCG | 1 cut(s) | 1837 |
| NdeII | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| NlaIII | CATG | 5 cut(s) | 510, 724, 1123, 1148, 1518 |
| NlaIV | GGNNCC | 1 cut(s) | 1355 |
| NmeAIII | GCCGAG | 1 cut(s) | 1005 |
| NmuCI | GTSAC | 4 cut(s) | 566, 643, 842, 1226 |
| NsbI | TGCGCA | 1 cut(s) | 450 |
| NspI | RCATGY | 1 cut(s) | 1148 |
| PaeR7I | CTCGAG | 3 cut(s) | 191, 470, 662 |
| PaqCI | CACCTGC | 1 cut(s) | 584 |
| PasI | CCCWGGG | 1 cut(s) | 1001 |
| PciSI | GCTCTTC | 1 cut(s) | 2069 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 28 |
| PctI | GAATGC | 1 cut(s) | 439 |
| PdmI | GAANNNNTTC | 2 cut(s) | 803, 1890 |
| PfeI | GAWTC | 3 cut(s) | 184, 196, 1687 |
| PkrI | GCNGC | 8 cut(s) | 106, 295, 601, 610, 903, 1711, 2023, 2062 |
| PleI | GAGTC | 1 cut(s) | 1333 |
| PpsI | GAGTC | 1 cut(s) | 1333 |
| Ppu21I | YACGTR | 1 cut(s) | 1438 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1343 |
| Psp6I | CCWGG | 2 cut(s) | 1000, 1222 |
| PspFI | CCCAGC | 1 cut(s) | 1913 |
| PspGI | CCWGG | 2 cut(s) | 1000, 1222 |
| PspN4I | GGNNCC | 1 cut(s) | 1355 |
| PspPI | GGNCC | 8 cut(s) | 503, 559, 746, 770, 819, 961, 1604, 1818 |
| PstI | CTGCAG | 1 cut(s) | 718 |
| PstNI | CAGNNNCTG | 3 cut(s) | 599, 1628, 2021 |
| PsuI | RGATCY | 3 cut(s) | 174, 1534, 1766 |
| PvuII | CAGCTG | 3 cut(s) | 493, 904, 1712 |
| RsaI | GTAC | 6 cut(s) | 88, 1115, 1384, 1442, 1496, 1988 |
| RsaNI | GTAC | 6 cut(s) | 87, 1114, 1383, 1441, 1495, 1987 |
| RseI | CAYNNNNRTG | 2 cut(s) | 444, 1308 |
| SapI | GCTCTTC | 1 cut(s) | 2069 |
| SaqAI | TTAA | 7 cut(s) | 201, 687, 1062, 1179, 1398, 1905, 1937 |
| SatI | GCNGC | 8 cut(s) | 105, 294, 600, 609, 902, 1710, 2022, 2061 |
| Sau3AI | GATC | 7 cut(s) | 174, 217, 222, 652, 892, 1534, 1766 |
| Sau96I | GGNCC | 8 cut(s) | 503, 559, 746, 770, 819, 961, 1604, 1818 |
| SchI | GAGTC | 1 cut(s) | 1333 |
| ScrFI | CCNGG | 2 cut(s) | 1002, 1224 |
| SduI | GDGCHC | 2 cut(s) | 1206, 1327 |
| SfaNI | GCATC | 5 cut(s) | 594, 1357, 1621, 1900, 1918 |
| SfcI | CTRYAG | 4 cut(s) | 714, 736, 1548, 2025 |
| Sfr274I | CTCGAG | 3 cut(s) | 191, 470, 662 |
| SinI | GGWCC | 3 cut(s) | 503, 770, 1604 |
| SlaI | CTCGAG | 3 cut(s) | 191, 470, 662 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 444, 1308 |
| SmlI | CTYRAG | 5 cut(s) | 191, 200, 392, 470, 662 |
| SmoI | CTYRAG | 5 cut(s) | 191, 200, 392, 470, 662 |
| SsiI | CCGC | 5 cut(s) | 138, 773, 1306, 1532, 1835 |
| SspI | AATATT | 1 cut(s) | 1183 |
| SspMI | CTAG | 3 cut(s) | 444, 582, 926 |
| StyD4I | CCNGG | 2 cut(s) | 1000, 1222 |
| StyI | CCWWGG | 1 cut(s) | 1498 |
| TaaI | ACNGT | 5 cut(s) | 91, 733, 854, 1353, 1624 |
| TaiI | ACGT | 5 cut(s) | 157, 236, 1178, 1440, 1780 |
| TaqI | TCGA | 5 cut(s) | 168, 192, 471, 588, 663 |
| TatI | WGTACW | 5 cut(s) | 86, 1113, 1382, 1440, 1986 |
| TfiI | GAWTC | 3 cut(s) | 184, 196, 1687 |
| Tru1I | TTAA | 7 cut(s) | 201, 687, 1062, 1179, 1398, 1905, 1937 |
| Tru9I | TTAA | 7 cut(s) | 201, 687, 1062, 1179, 1398, 1905, 1937 |
| TscAI | CASTG | 2 cut(s) | 1235, 1658 |
| TseFI | GTSAC | 4 cut(s) | 566, 643, 842, 1226 |
| TseI | GCWGC | 8 cut(s) | 104, 293, 599, 608, 901, 1709, 2021, 2060 |
| Tsp45I | GTSAC | 4 cut(s) | 566, 643, 842, 1226 |
| TspDTI | ATGAA | 7 cut(s) | 138, 377, 1048, 1229, 1417, 1652, 1734 |
| TspGWI | ACGGA | 2 cut(s) | 1403, 2059 |
| TspRI | CASTG | 2 cut(s) | 1235, 1658 |
| Vha464I | CTTAAG | 1 cut(s) | 200 |
| VpaK11BI | GGWCC | 3 cut(s) | 503, 770, 1604 |
| XapI | RAATTY | 4 cut(s) | 404, 1646, 2099, 2120 |
| XbaI | TCTAGA | 1 cut(s) | 925 |
| XceI | RCATGY | 1 cut(s) | 1148 |
| XhoI | CTCGAG | 3 cut(s) | 191, 470, 662 |
| XmiI | GTMKAC | 2 cut(s) | 735, 1735 |
| XmnI | GAANNNNTTC | 2 cut(s) | 803, 1890 |
| XspI | CTAG | 3 cut(s) | 444, 582, 926 |