Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

36161973 .. 36168889

Gene Structure 6917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g47820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2241 bp

ATGCGTCTGAAGACTCTGAAGGTGTTTATTCCATCTTCTTCCTCAGTTTGTCTCGTCCTAATTGGCACTATAGGAGGCTACTTCTTAGGAAGAGCCAGAGGTCGAAAAGCAGCACTCGTTGATCAGCAACAAGATGATGTAGCTTCCGTTGCGGACCTTCTCGAAAACAATGTGCAACCACCACCAGCTGCCGCCGTGGTTTCTCTATCTAAAGACGAACAGAAGGCCATTATCAGCAGAAAAATCAAACTTTTTGAGTCCATCCAGGCCGAACAAGAAGAACCCATTCAGTCTCTACCAGCAGAATCCATCAAGATTACATTGTGGTATGGCCAAGTGAAGGAGGTGAAGGAAGGGAAGAAGTGGATAACTTCCCCTTTGGACATAGCTGAGGAGGTTTCGGAGAGTTTTGCTTCGAAAGCTTTGGTTGCTGAGGTCAATGGGACACCATGGGACATGTCAAAGCCTTTGGAGTGTGACTGTGAGCTTAAGATATTCACATTTGAAGACAACAAAGGCCGTGACACATTCTGGCACTCGAGTTCTCATATTCTTGGACATGCACTTGAAGCCGAGTTTGGATGCAAGCTCTGCATTGGTCCATGCACAACAAAACAAGAGGGGTTCTACTATGATGCATATTACGGAGGTTTGGGGTTGAATGATGAGCACTTGAAGAAAATTGAAGCATTGGCAAAGAACGCTGTCGAGGCGAAACATTGCTTCAAGGACATTGAAGTATCTGTGGAACAGGCTCTTGAGATTTTCTCTGATAATAAATTCAAGGTTGAGATCATCAATGAGCTTCATGCAGACAACAAGGCCATAATTGTTTACAGATGTGGCGACTTGGTGGACTTGTGCCCCGGACCTCATATACCAAACACTTCCTTTGTCAAGGCATTTAGATGTTTGAAGGCTTCTTCAGCTTACTGGAGGGGAAATAAACATAGCGAAAGTCTGCAGCGAGTTTATGGAATATCTTATCCAGATGAAAATAGTTTGAAGGCTTATATCAAGCAGCAGGAAGAAGCAAAAAAGTATGACCATAGGGTGTTGGCTAAATCACAGGAGCTCATGTTTTGTCTCCCAAATAGCCCCGGAAGTTGGTTCTTCGCCCCAAACGGAACTCGGATTTATAAGAAGTTAATGCAGTTCATGGAAAAGGAGTATCTGAAAAGAGGATATCAGGAGGTTATAACACCAAACATGTTCAAAATGGATTTGTTTGAAAGATCTAAGCATGCAGGACACTACAAAGAGAATATGTTTCTTTTAGACATTGAGGAAGATGAATATGGTGTGAAGCCAATGAACTGTCCTGGTCACTGTGAAGTGTTTCGACATAGGCCTCATTCTTATAGAGAACTTCCTCTTCAGATAGCTGAATTTGGAGTGCTGCATCGGCATGAGGCCAGTGGTGCACTCGATTGCTTGACTCGTGTGAGGAGATTTGTGCAGGATGATGCTCATATATTTTGTCGGGAGTCACAGCTGAAAGATGAGCTCAAGGGTGTCCTTGACTTCATCAAGTATACATATGACATATTTGGTCTCTCTTTTGATGTAGAGTTATCCACAAGGCCGGAAAGTTACCATGGAGGAAGAGCAACATGGGATAAAGCCGAGGCGATTCTCACAGAAGCACTAAATGATTTTGGCATGCCTTGGCAGATAAATGAAGGGGATGGAGCATTTTACGGACCAAAGATAGATTTCGGCGTGTGTAATGCTTTGAAGAAGAAATTTCAGTGTGCAACAATACAGCTTGATTTTCAGCTACCGGATTGTGCGCAGTTCGACCTGCATTACACTTCAGATAAACAAGGCAAGGGGGAGAAACCGGTTATGATTCACAGAGCTGTATTCGGCTCCTTTGAGCGTTTCATTGGTATACTTTTGGAGCACTACAAAGGGAAATGGCCCTTCTGGCTTAGTCCACGGCAGTTAATTGTTTGCTCAGTCACACAGGAATTTGAGGCTTATGCTGTTCAGGTTCAGGAAAGGATTCGTCGCGATGGTTATCATGTTGATGTTGACATCAGTGATAGATCAATGAGTAAAAAGATTAGAGAAGCTCAGGTGGCACAATACAACTATGTTTTAGTTGTTGGTGACAAGGAAGCCAAAAGTGACCAGGTGAGTGTACGAGCTAGAGATAAACAAGATGAACTTATGGGAGTAATGAGTATCGATGATCTTCTCAGAGAACTGAAAAACAAAGTTGAAGCTTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

747

Amino Acids

85.2

Weight (kDa)

6.07

Isoelectric Point (pI)

39.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	104 - 167	6.1e-12	TGS domain
tRNA_SAD	PF07973	278 - 325	1.2e-11	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	427 - 637	1.2e-41	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	649 - 740	8.9e-19	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1140, 1199
Acc16I	TGCGCA	1 cut(s)	1794
Acc36I	ACCTGC	1 cut(s)	1812
AccI	GTMKAC	2 cut(s)	1535, 1894
AccII	CGCG	1 cut(s)	2016
AciI	CCGC	2 cut(s)	152, 192
AcoI	YGGCCR	1 cut(s)	331
AcsI	RAATTY	4 cut(s)	779, 1388, 1745, 1973
AcuI	CTGAAG	5 cut(s)	29, 38, 909, 1361, 1800
AfaI	GTAC	1 cut(s)	2148
AfiI	CCNNNNNNNGG	2 cut(s)	340, 1107
AflII	CTTAAG	1 cut(s)	488
AflIII	ACRYGT	2 cut(s)	456, 1209
AgeI	ACCGGT	1 cut(s)	1843
AjnI	CCWGG	3 cut(s)	264, 1321, 2136
AjuI	GAANNNNNNNTTGG	2 cut(s)	561, 593
Alw21I	GWGCWC	5 cut(s)	672, 1077, 1426, 1509, 1908
Alw26I	GTCTC	4 cut(s)	56, 297, 1091, 1559
Alw44I	GTGCAC	1 cut(s)	1422
Ama87I	CYCGRG	1 cut(s)	538
AoxI	GGCC	9 cut(s)	225, 267, 331, 517, 822, 1349, 1413, 1583, 1922
ApaLI	GTGCAC	1 cut(s)	1422
ApeKI	GCWGC	5 cut(s)	110, 188, 964, 1021, 1399
ApoI	RAATTY	4 cut(s)	779, 1388, 1745, 1973
AsiGI	ACCGGT	1 cut(s)	1843
Asp700I	GAANNNNTTC	3 cut(s)	285, 1338, 2007
AspLEI	GCGC	1 cut(s)	1795
AspS9I	GGNCC	5 cut(s)	154, 599, 869, 1703, 1923
AsuC2I	CCSGG	2 cut(s)	867, 1101
AsuHPI	GGTGA	3 cut(s)	358, 2126, 2152
AsuII	TTCGAA	1 cut(s)	416
AvaI	CYCGRG	1 cut(s)	538
AvaII	GGWCC	4 cut(s)	154, 599, 869, 1703
BaeGI	GKGCMC	2 cut(s)	866, 1426
BalI	TGGCCA	1 cut(s)	333
BanII	GRGCYC	2 cut(s)	1077, 1509
BauI	CACGAG	1 cut(s)	1440
BbsI	GAAGAC	2 cut(s)	17, 513
Bbv12I	GWGCWC	5 cut(s)	672, 1077, 1426, 1509, 1908
BbvCI	CCTCAGC	2 cut(s)	390, 432
BbvI	GCAGC	5 cut(s)	122, 175, 976, 1033, 1386
BccI	CCATC	5 cut(s)	40, 269, 317, 1682, 2012
BceAI	ACGGC	3 cut(s)	179, 504, 1958
BciT130I	CCWGG	3 cut(s)	266, 1323, 2138
BclI	TGATCA	1 cut(s)	121
BcnI	CCSGG	2 cut(s)	867, 1101
BcoDI	GTCTC	4 cut(s)	56, 297, 1091, 1559
BfaI	CTAG	1 cut(s)	2154
BfmI	CTRYAG	2 cut(s)	69, 962
BfrI	CTTAAG	1 cut(s)	488
BfuAI	ACCTGC	1 cut(s)	1812
BglI	GCCNNNNNGGC	1 cut(s)	1930
BglII	AGATCT	1 cut(s)	1235
BisI	GCNGC	6 cut(s)	111, 189, 192, 965, 1022, 1400
BlsI	GCNGC	6 cut(s)	112, 190, 193, 966, 1023, 1401
Bme1390I	CCNGG	5 cut(s)	266, 867, 1101, 1323, 2138
Bme18I	GGWCC	4 cut(s)	154, 599, 869, 1703
BmeT110I	CYCGRG	1 cut(s)	538
BmgT120I	GGNCC	5 cut(s)	154, 599, 869, 1703, 1923
BmiI	GGNNCC	1 cut(s)	1873
BmrFI	CCNGG	5 cut(s)	266, 867, 1101, 1323, 2138
BmsI	GCATC	4 cut(s)	572, 625, 1411, 1456
BpiI	GAAGAC	2 cut(s)	17, 513
BplI	GAGNNNNNCTC	2 cut(s)	752, 784
BpmI	CTGGAG	1 cut(s)	955
Bpu10I	CCTNAGC	3 cut(s)	390, 432, 2079
Bpu14I	TTCGAA	1 cut(s)	416
BpuEI	CTTGAG	2 cut(s)	779, 1493
BpuMI	CCSGG	2 cut(s)	867, 1101
Bsa29I	ATCGAT	1 cut(s)	2193
BsaBI	GATNNNNATC	1 cut(s)	2022
BsaI	GGTCTC	1 cut(s)	1559
BsaJI	CCNNGG	8 cut(s)	195, 449, 865, 1099, 1597, 1626, 1667, 1940
BsaWI	WCCGGW	2 cut(s)	1783, 1843
BsaXI	ACNNNNNCTCC	2 cut(s)	1064, 1094
Bsc4I	CCNNNNNNNGG	2 cut(s)	340, 1107
Bse118I	RCCGGY	1 cut(s)	1843
Bse1I	ACTGG	2 cut(s)	938, 1416
Bse3DI	GCAATG	1 cut(s)	718
Bse8I	GATNNNNATC	1 cut(s)	2022
BseBI	CCWGG	3 cut(s)	266, 1323, 2138
BseCI	ATCGAT	1 cut(s)	2193
BseDI	CCNNGG	8 cut(s)	195, 449, 865, 1099, 1597, 1626, 1667, 1940
BseGI	GGATG	4 cut(s)	261, 587, 1468, 1693
BseJI	GATNNNNATC	1 cut(s)	2022
BseLI	CCNNNNNNNGG	2 cut(s)	340, 1107
BseMI	GCAATG	1 cut(s)	718
BseMII	CTCAG	6 cut(s)	57, 381, 423, 1974, 2093, 2218
BseNI	ACTGG	2 cut(s)	938, 1416
BseRI	GAGGAG	2 cut(s)	407, 1462
BseSI	GKGCMC	2 cut(s)	866, 1426
BseXI	GCAGC	5 cut(s)	122, 175, 976, 1033, 1386
BsgI	GTGCAG	1 cut(s)	1478
Bsh1236I	CGCG	1 cut(s)	2016
BshFI	GGCC	9 cut(s)	227, 269, 333, 519, 824, 1351, 1415, 1585, 1924
BshTI	ACCGGT	1 cut(s)	1843
BshVI	ATCGAT	1 cut(s)	2193
BsiHKAI	GWGCWC	5 cut(s)	672, 1077, 1426, 1509, 1908
BsiHKCI	CYCGRG	1 cut(s)	538
BsiSI	CCGG	5 cut(s)	867, 1101, 1586, 1784, 1844
BslFI	GGGAC	2 cut(s)	457, 467
BslI	CCNNNNNNNGG	2 cut(s)	340, 1107
BsmAI	GTCTC	4 cut(s)	56, 297, 1091, 1559
BsmFI	GGGAC	2 cut(s)	457, 467
BsnI	GGCC	9 cut(s)	227, 269, 333, 519, 824, 1351, 1415, 1585, 1924
Bso31I	GGTCTC	1 cut(s)	1559
BsoBI	CYCGRG	1 cut(s)	538
Bsp119I	TTCGAA	1 cut(s)	416
Bsp1286I	GDGCHC	6 cut(s)	672, 866, 1077, 1426, 1509, 1908
Bsp143I	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
Bsp19I	CCATGG	2 cut(s)	449, 1597
Bsp68I	TCGCGA	1 cut(s)	2016
BspACI	CCGC	2 cut(s)	152, 192
BspANI	GGCC	9 cut(s)	227, 269, 333, 519, 824, 1351, 1415, 1585, 1924
BspCNI	CTCAG	6 cut(s)	56, 382, 424, 1973, 2092, 2217
BspDI	ATCGAT	1 cut(s)	2193
BspFNI	CGCG	1 cut(s)	2016
BspLI	GGNNCC	1 cut(s)	1873
BspMAI	CTGCAG	1 cut(s)	966
BspMI	ACCTGC	1 cut(s)	1812
BspQI	GCTCTTC	2 cut(s)	85, 1600
BspT104I	TTCGAA	1 cut(s)	416
BspTI	CTTAAG	1 cut(s)	488
BspTNI	GGTCTC	1 cut(s)	1559
BsrDI	GCAATG	1 cut(s)	718
BsrFI	RCCGGY	1 cut(s)	1843
BsrI	ACTGG	2 cut(s)	938, 1416
BssAI	RCCGGY	1 cut(s)	1843
BssECI	CCNNGG	8 cut(s)	195, 449, 865, 1099, 1597, 1626, 1667, 1940
BssMI	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
BssNAI	GTATAC	2 cut(s)	1536, 1895
BssSI	CACGAG	1 cut(s)	1440
BssT1I	CCWWGG	3 cut(s)	449, 1597, 1667
Bst1107I	GTATAC	2 cut(s)	1536, 1895
Bst2BI	CACGAG	1 cut(s)	1440
Bst2UI	CCWGG	3 cut(s)	266, 1323, 2138
Bst4CI	ACNGT	3 cut(s)	482, 1319, 1331
Bst6I	CTCTTC	3 cut(s)	85, 1380, 1600
BstAFI	CTTAAG	1 cut(s)	488
BstAPI	GCANNNNNTGC	1 cut(s)	591
BstBI	TTCGAA	1 cut(s)	416
BstC8I	GCNNGC	3 cut(s)	587, 1245, 1664
BstDEI	CTNAG	9 cut(s)	43, 85, 390, 432, 1239, 1934, 1960, 2079, 2204
BstDSI	CCRYGG	4 cut(s)	195, 449, 1597, 1940
BstF5I	GGATG	4 cut(s)	261, 587, 1468, 1693
BstFNI	CGCG	1 cut(s)	2016
BstHHI	GCGC	1 cut(s)	1795
BstKTI	GATC	5 cut(s)	124, 795, 1238, 2054, 2200
BstMAI	GTCTC	4 cut(s)	56, 297, 1091, 1559
BstMBI	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
BstNI	CCWGG	3 cut(s)	266, 1323, 2138
BstNSI	RCATGY	5 cut(s)	460, 563, 1213, 1247, 1666
BstSCI	CCNGG	5 cut(s)	264, 865, 1099, 1321, 2136
BstSFI	CTRYAG	2 cut(s)	69, 962
BstSLI	GKGCMC	2 cut(s)	866, 1426
BstUI	CGCG	1 cut(s)	2016
BstV1I	GCAGC	5 cut(s)	122, 175, 976, 1033, 1386
BstV2I	GAAGAC	2 cut(s)	17, 513
BstX2I	RGATCY	1 cut(s)	1235
BstYI	RGATCY	1 cut(s)	1235
BstZ17I	GTATAC	2 cut(s)	1536, 1895
Bsu15I	ATCGAT	1 cut(s)	2193
BsuRI	GGCC	9 cut(s)	227, 269, 333, 519, 824, 1351, 1415, 1585, 1924
BsuTUI	ATCGAT	1 cut(s)	2193
BtgI	CCRYGG	4 cut(s)	195, 449, 1597, 1940
BtgZI	GCGATG	1 cut(s)	2031
BtsCI	GGATG	4 cut(s)	261, 587, 1468, 1693
BtsIMutI	CAGTG	4 cut(s)	1327, 1423, 1757, 2050
BtuMI	TCGCGA	1 cut(s)	2016
BveI	ACCTGC	1 cut(s)	1812
Cac8I	GCNNGC	3 cut(s)	587, 1245, 1664
CfoI	GCGC	1 cut(s)	1795
Cfr10I	RCCGGY	1 cut(s)	1843
Cfr13I	GGNCC	5 cut(s)	154, 599, 869, 1703, 1923
ClaI	ATCGAT	1 cut(s)	2193
CsiI	ACCWGGT	1 cut(s)	2136
Csp6I	GTAC	1 cut(s)	2147
CspAI	ACCGGT	1 cut(s)	1843
CviQI	GTAC	1 cut(s)	2147
DdeI	CTNAG	9 cut(s)	43, 85, 390, 432, 1239, 1934, 1960, 2079, 2204
DpnI	GATC	5 cut(s)	123, 794, 1237, 2053, 2199
DpnII	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
EaeI	YGGCCR	1 cut(s)	331
Eam1104I	CTCTTC	3 cut(s)	85, 1380, 1600
EarI	CTCTTC	3 cut(s)	85, 1380, 1600
Ecl136II	GAGCTC	2 cut(s)	1075, 1507
Eco130I	CCWWGG	3 cut(s)	449, 1597, 1667
Eco147I	AGGCCT	1 cut(s)	1351
Eco24I	GRGCYC	2 cut(s)	1077, 1509
Eco31I	GGTCTC	1 cut(s)	1559
Eco32I	GATATC	1 cut(s)	1187
Eco47I	GGWCC	4 cut(s)	154, 599, 869, 1703
Eco53kI	GAGCTC	2 cut(s)	1075, 1507
Eco57I	CTGAAG	5 cut(s)	29, 38, 909, 1361, 1800
Eco88I	CYCGRG	1 cut(s)	538
EcoICRI	GAGCTC	2 cut(s)	1075, 1507
EcoRII	CCWGG	3 cut(s)	264, 1321, 2136
EcoRV	GATATC	1 cut(s)	1187
EcoT14I	CCWWGG	3 cut(s)	449, 1597, 1667
EcoT22I	ATGCAT	1 cut(s)	640
EcoT38I	GRGCYC	2 cut(s)	1077, 1509
ErhI	CCWWGG	3 cut(s)	449, 1597, 1667
FalI	AAGNNNNNCTT	2 cut(s)	2157, 2189
FaqI	GGGAC	2 cut(s)	457, 467
FauNDI	CATATG	1 cut(s)	1540
FbaI	TGATCA	1 cut(s)	121
FblI	GTMKAC	2 cut(s)	1535, 1894
Fnu4HI	GCNGC	6 cut(s)	111, 189, 192, 965, 1022, 1400
FokI	GGATG	4 cut(s)	248, 594, 1475, 1700
FriOI	GRGCYC	2 cut(s)	1077, 1509
Fsp4HI	GCNGC	6 cut(s)	111, 189, 192, 965, 1022, 1400
FspBI	CTAG	1 cut(s)	2154
FspI	TGCGCA	1 cut(s)	1794
GlaI	GCGC	1 cut(s)	1794
GluI	GCNGC	6 cut(s)	111, 189, 192, 965, 1022, 1400
GsuI	CTGGAG	1 cut(s)	955
HaeIII	GGCC	9 cut(s)	227, 269, 333, 519, 824, 1351, 1415, 1585, 1924
HapII	CCGG	5 cut(s)	867, 1101, 1586, 1784, 1844
HhaI	GCGC	1 cut(s)	1795
Hin6I	GCGC	1 cut(s)	1793
HinP1I	GCGC	1 cut(s)	1793
HincII	GTYRAC	1 cut(s)	2038
HindII	GTYRAC	1 cut(s)	2038
HindIII	AAGCTT	2 cut(s)	420, 2229
HinfI	GANTC	8 cut(s)	13, 257, 305, 1438, 1487, 1633, 1852, 2008
HpaII	CCGG	5 cut(s)	867, 1101, 1586, 1784, 1844
HphI	GGTGA	3 cut(s)	358, 2126, 2152
Hpy166II	GTNNAC	8 cut(s)	835, 856, 1424, 1536, 1895, 1940, 2038, 2147
Hpy188I	TCNGA	9 cut(s)	9, 18, 403, 772, 1134, 1176, 1380, 1819, 2207
Hpy188III	TCNNGA	8 cut(s)	161, 313, 758, 989, 1190, 1484, 2000, 2015
Hpy8I	GTNNAC	8 cut(s)	835, 856, 1424, 1536, 1895, 1940, 2038, 2147
Hpy99I	CGWCG	1 cut(s)	2016
HpyCH4III	ACNGT	3 cut(s)	482, 1319, 1331
HpyF3I	CTNAG	9 cut(s)	43, 85, 390, 432, 1239, 1934, 1960, 2079, 2204
HspAI	GCGC	1 cut(s)	1793
Ksp22I	TGATCA	1 cut(s)	121
Kzo9I	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
LguI	GCTCTTC	2 cut(s)	85, 1600
LmnI	GCTCC	4 cut(s)	1072, 1691, 1877, 1903
Lsp1109I	GCAGC	5 cut(s)	122, 175, 976, 1033, 1386
LweI	GCATC	4 cut(s)	572, 625, 1411, 1456
MabI	ACCWGGT	1 cut(s)	2136
MaeI	CTAG	1 cut(s)	2154
MaeIII	GTNAC	8 cut(s)	476, 521, 1325, 1488, 1592, 1963, 2114, 2132
MalI	GATC	5 cut(s)	123, 794, 1237, 2053, 2199
MboI	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
MflI	RGATCY	1 cut(s)	1235
MhlI	GDGCHC	6 cut(s)	672, 866, 1077, 1426, 1509, 1908
MlsI	TGGCCA	1 cut(s)	333
MluCI	AATT	8 cut(s)	60, 681, 779, 828, 1388, 1745, 1950, 1973
MluNI	TGGCCA	1 cut(s)	333
MlyI	GAGTC	4 cut(s)	7, 266, 1432, 1496
Mox20I	TGGCCA	1 cut(s)	333
Mph1103I	ATGCAT	1 cut(s)	640
MroXI	GAANNNNTTC	3 cut(s)	285, 1338, 2007
MscI	TGGCCA	1 cut(s)	333
MseI	TTAA	3 cut(s)	489, 1148, 1949
MslI	CAYNNNNRTG	3 cut(s)	907, 1407, 2031
Msp20I	TGGCCA	1 cut(s)	333
MspA1I	CMGCKG	2 cut(s)	188, 1495
MspCI	CTTAAG	1 cut(s)	488
MspI	CCGG	5 cut(s)	867, 1101, 1586, 1784, 1844
MspR9I	CCNGG	5 cut(s)	266, 867, 1101, 1323, 2138
MvaI	CCWGG	3 cut(s)	266, 1323, 2138
MvnI	CGCG	1 cut(s)	2016
NciI	CCSGG	2 cut(s)	867, 1101
NcoI	CCATGG	2 cut(s)	449, 1597
NdeI	CATATG	1 cut(s)	1540
NdeII	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
NlaIV	GGNNCC	1 cut(s)	1873
NmeAIII	GCCGAG	2 cut(s)	598, 1651
NmuCI	GTSAC	7 cut(s)	476, 521, 1325, 1488, 1963, 2114, 2132
NruI	TCGCGA	1 cut(s)	2016
NsbI	TGCGCA	1 cut(s)	1794
NsiI	ATGCAT	1 cut(s)	640
NspI	RCATGY	5 cut(s)	460, 563, 1213, 1247, 1666
NspV	TTCGAA	1 cut(s)	416
PaeI	GCATGC	2 cut(s)	1247, 1666
PaeR7I	CTCGAG	1 cut(s)	538
PceI	AGGCCT	1 cut(s)	1351
PciI	ACATGT	2 cut(s)	456, 1209
PciSI	GCTCTTC	2 cut(s)	85, 1600
PdmI	GAANNNNTTC	3 cut(s)	285, 1338, 2007
PfeI	GAWTC	4 cut(s)	305, 1633, 1852, 2008
PinAI	ACCGGT	1 cut(s)	1843
PkrI	GCNGC	6 cut(s)	112, 190, 193, 966, 1023, 1401
PleI	GAGTC	4 cut(s)	7, 265, 1432, 1495
PpsI	GAGTC	4 cut(s)	7, 265, 1432, 1495
PscI	ACATGT	2 cut(s)	456, 1209
PsiI	TTATAA	2 cut(s)	1140, 1199
Psp124BI	GAGCTC	2 cut(s)	1077, 1509
Psp6I	CCWGG	3 cut(s)	264, 1321, 2136
PspGI	CCWGG	3 cut(s)	264, 1321, 2136
PspN4I	GGNNCC	1 cut(s)	1873
PspPI	GGNCC	5 cut(s)	154, 599, 869, 1703, 1923
PspXI	VCTCGAGB	1 cut(s)	538
PstI	CTGCAG	1 cut(s)	966
PsuI	RGATCY	1 cut(s)	1235
PvuII	CAGCTG	2 cut(s)	188, 1495
RruI	TCGCGA	1 cut(s)	2016
RsaI	GTAC	1 cut(s)	2148
RsaNI	GTAC	1 cut(s)	2147
RseI	CAYNNNNRTG	3 cut(s)	907, 1407, 2031
SacI	GAGCTC	2 cut(s)	1077, 1509
SapI	GCTCTTC	2 cut(s)	85, 1600
SaqAI	TTAA	3 cut(s)	489, 1148, 1949
SatI	GCNGC	6 cut(s)	111, 189, 192, 965, 1022, 1400
Sau3AI	GATC	5 cut(s)	121, 792, 1235, 2051, 2197
Sau96I	GGNCC	5 cut(s)	154, 599, 869, 1703, 1923
SchI	GAGTC	4 cut(s)	7, 266, 1432, 1496
ScrFI	CCNGG	5 cut(s)	266, 867, 1101, 1323, 2138
SduI	GDGCHC	6 cut(s)	672, 866, 1077, 1426, 1509, 1908
SexAI	ACCWGGT	1 cut(s)	2136
SfaNI	GCATC	4 cut(s)	572, 625, 1411, 1456
SfcI	CTRYAG	2 cut(s)	69, 962
Sfr274I	CTCGAG	1 cut(s)	538
SfuI	TTCGAA	1 cut(s)	416
SinI	GGWCC	4 cut(s)	154, 599, 869, 1703
SlaI	CTCGAG	1 cut(s)	538
SmiMI	CAYNNNNRTG	3 cut(s)	907, 1407, 2031
SmlI	CTYRAG	4 cut(s)	488, 538, 758, 1508
SmoI	CTYRAG	4 cut(s)	488, 538, 758, 1508
SphI	GCATGC	2 cut(s)	1247, 1666
Sse9I	AATT	8 cut(s)	60, 681, 779, 828, 1388, 1745, 1950, 1973
SseBI	AGGCCT	1 cut(s)	1351
SsiI	CCGC	2 cut(s)	152, 192
SspMI	CTAG	1 cut(s)	2154
SstI	GAGCTC	2 cut(s)	1077, 1509
StuI	AGGCCT	1 cut(s)	1351
StyD4I	CCNGG	5 cut(s)	264, 865, 1099, 1321, 2136
StyI	CCWWGG	3 cut(s)	449, 1597, 1667
TaaI	ACNGT	3 cut(s)	482, 1319, 1331
TaqI	TCGA	9 cut(s)	103, 162, 416, 539, 708, 1342, 1428, 1800, 2193
TasI	AATT	8 cut(s)	60, 681, 779, 828, 1388, 1745, 1950, 1973
TauI	GCSGC	1 cut(s)	194
TfiI	GAWTC	4 cut(s)	305, 1633, 1852, 2008
Tru1I	TTAA	3 cut(s)	489, 1148, 1949
Tru9I	TTAA	3 cut(s)	489, 1148, 1949
TscAI	CASTG	4 cut(s)	1334, 1423, 1757, 2050
TseFI	GTSAC	7 cut(s)	476, 521, 1325, 1488, 1963, 2114, 2132
TseI	GCWGC	5 cut(s)	110, 188, 964, 1021, 1399
Tsp45I	GTSAC	7 cut(s)	476, 521, 1325, 1488, 1963, 2114, 2132
TspDTI	ATGAA	9 cut(s)	797, 1008, 1147, 1308, 1328, 1516, 1695, 1876, 2184
TspGWI	ACGGA	4 cut(s)	136, 660, 1140, 1716
TspRI	CASTG	4 cut(s)	1334, 1423, 1757, 2050
Vha464I	CTTAAG	1 cut(s)	488
VneI	GTGCAC	1 cut(s)	1422
VpaK11BI	GGWCC	4 cut(s)	154, 599, 869, 1703
XapI	RAATTY	4 cut(s)	779, 1388, 1745, 1973
XceI	RCATGY	5 cut(s)	460, 563, 1213, 1247, 1666
XhoI	CTCGAG	1 cut(s)	538
XmiI	GTMKAC	2 cut(s)	1535, 1894
XmnI	GAANNNNTTC	3 cut(s)	285, 1338, 2007
XspI	CTAG	1 cut(s)	2154
Zsp2I	ATGCAT	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.