Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

15412675 .. 15415914

Gene Structure 3240 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032456

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGCACATGGAGGTGTTGATCCCAGCTTTCTCAGTTTTGTTCATCATAATTGGTACTTTAGGAAGCTACTTCTTCGGAAGAGCCAGAGGTCGAGAAGATCGAATCGCAGTACTCTCCGATCAACCACAACCTGTAACTTCCGTGGTGGACCGGGCCAAAAACGATGTGCAACCACCACCAGTCACCACCATGGTTTCCCAACCCAAAGACGAGGCCACCATGGAGTCCGTTATCCCCAAAAGAATCAAACTTTTCAAGTCCATCCAGGCCCAACAAAAGGCCCACATTCAGTCTCTTCCCCCGGAATTCATCAAATCTATCAATGGTCTGATATGGTTGCAGGCACTTGAGGCCAAGTATGGATGTAAGCTCTGTATTGGTCCCTGCCCAGCAAAAGAAGATGGGTTCTACTATGATGCATACTATGGCAGTTTGGGCTTGAATGATGAGCACTTAAAGAAAATTGAATCAGAGGTAGAGAAGGCTGTTAAGGCCAAACAGTGCTTTGAGCGCATTGAAGTATCAATGGAACAGGCCCTCGAGATTTTCTCTGATAACAAATTCAAGATTGAGATCATCAATGAGCTTCCTGTAGATAAGGCTATAACTGTATACAGATGTGGCAACTTGGTGGACTTGTGCCGTGGACCTCATATCCCAAATACATCCTTTGTCAAAGCATTTAGATGTTTGAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGCGAAAGTCTGCACCCGGGAAGTTGGTTCTTCACCCCCAATGGAGCTCGGATTTATAAGAAATTAATGCAGTTCATGGAAAATGAATATTTGGAAAGAGGATATCAGGAGGTTATAACACCAAACATATTCAAAATGGATTTGTTTGAAAAATCTAAGCATGCAGGACACTACAAACAGAATATGTTTCTTTTAGAGACTGAGAAAGAAGAATATGGTTTGAAGCCAATGAACTGTCCTGGTCACTGTGAAGAGTTTCGACATAGGCCTCGTTCTTATAGAGAACTTCCTCTTCGGATAGCTGAATTTGGGGTGCTGCATCGAAATGAGGTCAATGGTGCACTAGATGGATTGACTCGTGTCAGGAGATTCGTGCAGGATGATGCTCATATATTTTGTAGGGAGTCCCAACTGAAAGATGAACTCAAGGGTGTCCTCGACTTCATGAAGTATACCTATGACATCTTTGGTCTCTCTTTTGATGTGGAGCTATCAACAAGGCGAGAAAGTTACCATGGAGAACAAGCAACATGGGATAAAGCCGAGGCAATTCTTACAGAAATAAATGAAGGGGATGGGGCATTTTATGGACCAAAGATAGATTTTGGTGTGTGTAATGCTTTGAAGAAAAAATTTCAGTGTGCTACAATACAGCTTGATTTTCAGCTCCCTGATTATGGTAAAGAAGGCAAGAGGGAGAGACCTGTTATGATACACAGAGCTGTATTCGGCTCCTTTGAGCGTTTCTTTGGTATACTTTTGGAGCACTACAAAGGAAAATGGCCCTTCTGGCTTAGTCCACGTCAGGCAATTGTTTGCTCCGTCTCAGAGAAATCCGAGGCTTATGCTCTACAGGTTCAGGAACAGATTCGTCGCGCTGGTTATCATGTTGATGTTGACACAAGTGATAGATCCGTGAGGAAAAAGGTGCGAGAAGCTCAAGTGGCACAATACAACTATGTTCTAGTTGTTGGTGAGAAGGCAGCCAAAATTGACCAGGTGAGCTTACGGGCTAGAGATACAGAAGATGAAGATGTCACAGTAATGAGCATCGATGATCTTCTCCAATTCTTCAAAGACGAAGTTGAAGCTTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

610

Amino Acids

70.19

Weight (kDa)

6.63

Isoelectric Point (pI)

37.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_SAD	PF07973	202 - 245	2.9e-11	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	308 - 500	2.3e-40	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	512 - 603	1.1e-16	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	786, 845
AccI	GTMKAC	3 cut(s)	612, 1181, 1483
AccII	CGCG	2 cut(s)	732, 1605
AclWI	GGATC	2 cut(s)	13, 1635
AcsI	RAATTY	4 cut(s)	305, 560, 1034, 1361
AcuI	CTGAAG	1 cut(s)	687
AfaI	GTAC	2 cut(s)	55, 111
AfiI	CCNNNNNNNGG	3 cut(s)	277, 714, 1406
AjiI	CACGTC	1 cut(s)	1532
AjnI	CCWGG	3 cut(s)	264, 967, 1725
Alw21I	GWGCWC	4 cut(s)	453, 778, 1072, 1497
Alw26I	GTCTC	5 cut(s)	297, 920, 1205, 1423, 1558
Alw44I	GTGCAC	1 cut(s)	1068
AlwI	GGATC	2 cut(s)	13, 1635
Ama87I	CYCGRG	2 cut(s)	539, 745
AoxI	GGCC	9 cut(s)	153, 213, 267, 279, 351, 492, 534, 995, 1511
ApaLI	GTGCAC	1 cut(s)	1068
ApeKI	GCWGC	2 cut(s)	1045, 1712
ApoI	RAATTY	4 cut(s)	305, 560, 1034, 1361
AseI	ATTAAT	1 cut(s)	794
Asp700I	GAANNNNTTC	2 cut(s)	984, 1596
AspLEI	GCGC	2 cut(s)	513, 1607
AspS9I	GGNCC	9 cut(s)	148, 153, 268, 280, 380, 535, 647, 1319, 1512
AsuC2I	CCSGG	4 cut(s)	152, 302, 746, 747
AsuHPI	GGTGA	4 cut(s)	175, 754, 1715, 1741
AvaI	CYCGRG	2 cut(s)	539, 745
AvaII	GGWCC	4 cut(s)	148, 380, 647, 1319
BaeGI	GKGCMC	1 cut(s)	1072
BanII	GRGCYC	1 cut(s)	778
BauI	CACGAG	1 cut(s)	1086
Bbv12I	GWGCWC	4 cut(s)	453, 778, 1072, 1497
BbvI	GCAGC	2 cut(s)	1032, 1724
BccI	CCATC	4 cut(s)	269, 395, 1070, 1298
BceAI	ACGGC	1 cut(s)	627
BcgI	CGANNNNNNTGC	2 cut(s)	1556, 1590
BciT130I	CCWGG	3 cut(s)	266, 969, 1727
BcnI	CCSGG	4 cut(s)	152, 302, 746, 747
BcoDI	GTCTC	5 cut(s)	297, 920, 1205, 1423, 1558
BfaI	CTAG	3 cut(s)	1073, 1694, 1743
BfmI	CTRYAG	2 cut(s)	591, 1580
BglI	GCCNNNNNGGC	1 cut(s)	1519
BisI	GCNGC	2 cut(s)	1046, 1713
BlsI	GCNGC	2 cut(s)	1047, 1714
BmcAI	AGTACT	1 cut(s)	111
Bme1390I	CCNGG	7 cut(s)	152, 266, 302, 746, 747, 969, 1727
Bme18I	GGWCC	4 cut(s)	148, 380, 647, 1319
BmeT110I	CYCGRG	2 cut(s)	539, 745
BmgBI	CACGTC	1 cut(s)	1532
BmgT120I	GGNCC	9 cut(s)	148, 153, 268, 280, 380, 535, 647, 1319, 1512
BmiI	GGNNCC	2 cut(s)	382, 1462
BmrFI	CCNGG	7 cut(s)	152, 266, 302, 746, 747, 969, 1727
BmsI	GCATC	4 cut(s)	406, 1057, 1102, 1788
BoxI	GACNNNNGTC	1 cut(s)	1088
BplI	GAGNNNNNCTC	2 cut(s)	533, 565
BpmI	CTGGAG	1 cut(s)	733
BpuEI	CTTGAG	3 cut(s)	368, 1139, 1653
BpuMI	CCSGG	4 cut(s)	152, 302, 746, 747
Bsa29I	ATCGAT	1 cut(s)	1782
BsaBI	GATNNNNATC	1 cut(s)	572
BsaI	GGTCTC	2 cut(s)	1205, 1423
BsaJI	CCNNGG	9 cut(s)	141, 189, 219, 300, 643, 745, 1243, 1272, 1566
Bsc4I	CCNNNNNNNGG	3 cut(s)	277, 714, 1406
Bse1I	ACTGG	2 cut(s)	179, 716
Bse8I	GATNNNNATC	1 cut(s)	572
BseBI	CCWGG	3 cut(s)	266, 969, 1727
BseCI	ATCGAT	1 cut(s)	1782
BseDI	CCNNGG	9 cut(s)	141, 189, 219, 300, 643, 745, 1243, 1272, 1566
BseGI	GGATG	5 cut(s)	261, 368, 665, 1114, 1309
BseJI	GATNNNNATC	1 cut(s)	572
BseLI	CCNNNNNNNGG	3 cut(s)	277, 714, 1406
BseMII	CTCAG	3 cut(s)	45, 921, 1569
BseNI	ACTGG	2 cut(s)	179, 716
BseSI	GKGCMC	1 cut(s)	1072
BseXI	GCAGC	2 cut(s)	1032, 1724
BseYI	CCCAGC	2 cut(s)	22, 388
BsgI	GTGCAG	2 cut(s)	725, 1124
Bsh1236I	CGCG	2 cut(s)	732, 1605
BshFI	GGCC	9 cut(s)	155, 215, 269, 281, 353, 494, 536, 997, 1513
BshVI	ATCGAT	1 cut(s)	1782
BsiHKAI	GWGCWC	4 cut(s)	453, 778, 1072, 1497
BsiHKCI	CYCGRG	2 cut(s)	539, 745
BsiSI	CCGG	3 cut(s)	151, 302, 746
BslFI	GGGAC	2 cut(s)	366, 1120
BslI	CCNNNNNNNGG	3 cut(s)	277, 714, 1406
BsmAI	GTCTC	5 cut(s)	297, 920, 1205, 1423, 1558
BsmBI	CGTCTC	1 cut(s)	1558
BsmFI	GGGAC	2 cut(s)	366, 1120
BsnI	GGCC	9 cut(s)	155, 215, 269, 281, 353, 494, 536, 997, 1513
Bso31I	GGTCTC	2 cut(s)	1205, 1423
BsoBI	CYCGRG	2 cut(s)	539, 745
Bsp1286I	GDGCHC	4 cut(s)	453, 778, 1072, 1497
Bsp143I	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
Bsp19I	CCATGG	3 cut(s)	189, 219, 1243
Bsp68I	TCGCGA	1 cut(s)	732
BspANI	GGCC	9 cut(s)	155, 215, 269, 281, 353, 494, 536, 997, 1513
BspCNI	CTCAG	3 cut(s)	44, 922, 1568
BspDI	ATCGAT	1 cut(s)	1782
BspFNI	CGCG	2 cut(s)	732, 1605
BspHI	TCATGA	1 cut(s)	1173
BspLI	GGNNCC	2 cut(s)	382, 1462
BspPI	GGATC	2 cut(s)	13, 1635
BspQI	GCTCTTC	1 cut(s)	73
BspTNI	GGTCTC	2 cut(s)	1205, 1423
BsrI	ACTGG	2 cut(s)	179, 716
BssECI	CCNNGG	9 cut(s)	141, 189, 219, 300, 643, 745, 1243, 1272, 1566
BssMI	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
BssNAI	GTATAC	3 cut(s)	613, 1182, 1484
BssSI	CACGAG	1 cut(s)	1086
BssT1I	CCWWGG	3 cut(s)	189, 219, 1243
Bst1107I	GTATAC	3 cut(s)	613, 1182, 1484
Bst2BI	CACGAG	1 cut(s)	1086
Bst2UI	CCWGG	3 cut(s)	266, 969, 1727
Bst4CI	ACNGT	5 cut(s)	501, 610, 965, 977, 1771
Bst6I	CTCTTC	4 cut(s)	73, 300, 975, 1026
BstC8I	GCNNGC	2 cut(s)	342, 891
BstDEI	CTNAG	5 cut(s)	31, 885, 930, 1523, 1555
BstDSI	CCRYGG	5 cut(s)	141, 189, 219, 643, 1243
BstENI	CCTNNNNNAGG	1 cut(s)	712
BstF5I	GGATG	5 cut(s)	261, 368, 665, 1114, 1309
BstFNI	CGCG	2 cut(s)	732, 1605
BstHHI	GCGC	2 cut(s)	513, 1607
BstKTI	GATC	7 cut(s)	21, 100, 121, 576, 730, 1643, 1789
BstMAI	GTCTC	5 cut(s)	297, 920, 1205, 1423, 1558
BstMBI	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
BstMWI	GCNNNNNNNGC	7 cut(s)	350, 435, 491, 510, 704, 1519, 1673
BstNI	CCWGG	3 cut(s)	266, 969, 1727
BstNSI	RCATGY	1 cut(s)	893
BstPAI	GACNNNNGTC	1 cut(s)	1088
BstSCI	CCNGG	7 cut(s)	150, 264, 300, 744, 745, 967, 1725
BstSFI	CTRYAG	2 cut(s)	591, 1580
BstSLI	GKGCMC	1 cut(s)	1072
BstUI	CGCG	2 cut(s)	732, 1605
BstV1I	GCAGC	2 cut(s)	1032, 1724
BstX2I	RGATCY	1 cut(s)	1640
BstYI	RGATCY	1 cut(s)	1640
BstZ17I	GTATAC	3 cut(s)	613, 1182, 1484
Bsu15I	ATCGAT	1 cut(s)	1782
BsuRI	GGCC	9 cut(s)	155, 215, 269, 281, 353, 494, 536, 997, 1513
BsuTUI	ATCGAT	1 cut(s)	1782
BtgI	CCRYGG	5 cut(s)	141, 189, 219, 643, 1243
BtrI	CACGTC	1 cut(s)	1532
BtsCI	GGATG	5 cut(s)	261, 368, 665, 1114, 1309
BtsIMutI	CAGTG	3 cut(s)	506, 973, 1373
BtuMI	TCGCGA	1 cut(s)	732
Cac8I	GCNNGC	2 cut(s)	342, 891
CciI	TCATGA	1 cut(s)	1173
CfoI	GCGC	2 cut(s)	513, 1607
Cfr13I	GGNCC	9 cut(s)	148, 153, 268, 280, 380, 535, 647, 1319, 1512
Cfr9I	CCCGGG	1 cut(s)	745
ClaI	ATCGAT	1 cut(s)	1782
CsiI	ACCWGGT	1 cut(s)	1725
Csp6I	GTAC	2 cut(s)	54, 110
CviAII	CATG	9 cut(s)	7, 190, 220, 805, 890, 1174, 1244, 1260, 1616
CviQI	GTAC	2 cut(s)	54, 110
DdeI	CTNAG	5 cut(s)	31, 885, 930, 1523, 1555
DpnI	GATC	7 cut(s)	20, 99, 120, 575, 729, 1642, 1788
DpnII	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
Eam1104I	CTCTTC	4 cut(s)	73, 300, 975, 1026
EarI	CTCTTC	4 cut(s)	73, 300, 975, 1026
Ecl136II	GAGCTC	1 cut(s)	776
Eco130I	CCWWGG	3 cut(s)	189, 219, 1243
Eco147I	AGGCCT	1 cut(s)	997
Eco24I	GRGCYC	1 cut(s)	778
Eco31I	GGTCTC	2 cut(s)	1205, 1423
Eco32I	GATATC	1 cut(s)	833
Eco47I	GGWCC	4 cut(s)	148, 380, 647, 1319
Eco53kI	GAGCTC	1 cut(s)	776
Eco57I	CTGAAG	1 cut(s)	687
Eco88I	CYCGRG	2 cut(s)	539, 745
EcoICRI	GAGCTC	1 cut(s)	776
EcoNI	CCTNNNNNAGG	1 cut(s)	712
EcoO109I	RGGNCCY	1 cut(s)	535
EcoRI	GAATTC	1 cut(s)	305
EcoRII	CCWGG	3 cut(s)	264, 967, 1725
EcoRV	GATATC	1 cut(s)	833
EcoT14I	CCWWGG	3 cut(s)	189, 219, 1243
EcoT22I	ATGCAT	1 cut(s)	421
EcoT38I	GRGCYC	1 cut(s)	778
ErhI	CCWWGG	3 cut(s)	189, 219, 1243
Esp3I	CGTCTC	1 cut(s)	1558
FaeI	CATG	9 cut(s)	10, 193, 223, 808, 893, 1177, 1247, 1263, 1619
FaqI	GGGAC	2 cut(s)	366, 1120
FatI	CATG	9 cut(s)	6, 189, 219, 804, 889, 1173, 1243, 1259, 1615
FblI	GTMKAC	3 cut(s)	612, 1181, 1483
Fnu4HI	GCNGC	2 cut(s)	1046, 1713
FokI	GGATG	5 cut(s)	248, 375, 652, 1121, 1316
FriOI	GRGCYC	1 cut(s)	778
Fsp4HI	GCNGC	2 cut(s)	1046, 1713
FspBI	CTAG	3 cut(s)	1073, 1694, 1743
GlaI	GCGC	2 cut(s)	512, 1606
GluI	GCNGC	2 cut(s)	1046, 1713
GsaI	CCCAGC	2 cut(s)	26, 392
GsuI	CTGGAG	1 cut(s)	733
HaeIII	GGCC	9 cut(s)	155, 215, 269, 281, 353, 494, 536, 997, 1513
HapII	CCGG	3 cut(s)	151, 302, 746
HhaI	GCGC	2 cut(s)	513, 1607
Hin1II	CATG	9 cut(s)	10, 193, 223, 808, 893, 1177, 1247, 1263, 1619
Hin6I	GCGC	2 cut(s)	511, 1605
HinP1I	GCGC	2 cut(s)	511, 1605
HincII	GTYRAC	1 cut(s)	1627
HindII	GTYRAC	1 cut(s)	1627
HindIII	AAGCTT	1 cut(s)	1818
HinfI	GANTC	8 cut(s)	102, 224, 243, 467, 1084, 1098, 1133, 1597
HpaII	CCGG	3 cut(s)	151, 302, 746
HphI	GGTGA	4 cut(s)	175, 754, 1715, 1741
Hpy166II	GTNNAC	9 cut(s)	148, 613, 634, 647, 1070, 1182, 1484, 1529, 1627
Hpy188I	TCNGA	9 cut(s)	77, 118, 330, 472, 553, 780, 1026, 1558, 1567
Hpy188III	TCNNGA	8 cut(s)	92, 541, 565, 731, 836, 1093, 1174, 1589
Hpy8I	GTNNAC	9 cut(s)	148, 613, 634, 647, 1070, 1182, 1484, 1529, 1627
Hpy99I	CGWCG	1 cut(s)	1605
HpyAV	CCTTC	6 cut(s)	475, 688, 1292, 1409, 1525, 1702
HpyCH4III	ACNGT	5 cut(s)	501, 610, 965, 977, 1771
HpyCH4IV	ACGT	1 cut(s)	1531
HpyF10VI	GCNNNNNNNGC	7 cut(s)	350, 435, 491, 510, 704, 1519, 1673
HpyF3I	CTNAG	5 cut(s)	31, 885, 930, 1523, 1555
HpySE526I	ACGT	1 cut(s)	1531
Hsp92II	CATG	9 cut(s)	10, 193, 223, 808, 893, 1177, 1247, 1263, 1619
HspAI	GCGC	2 cut(s)	511, 1605
Kzo9I	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
LguI	GCTCTTC	1 cut(s)	73
LmnI	GCTCC	6 cut(s)	773, 1216, 1401, 1466, 1492, 1553
Lsp1109I	GCAGC	2 cut(s)	1032, 1724
LweI	GCATC	4 cut(s)	406, 1057, 1102, 1788
MabI	ACCWGGT	1 cut(s)	1725
MaeI	CTAG	3 cut(s)	1073, 1694, 1743
MaeII	ACGT	1 cut(s)	1531
MaeIII	GTNAC	5 cut(s)	133, 181, 971, 1238, 1765
MalI	GATC	7 cut(s)	20, 99, 120, 575, 729, 1642, 1788
MboI	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
MfeI	CAATTG	1 cut(s)	1539
MflI	RGATCY	1 cut(s)	1640
MhlI	GDGCHC	4 cut(s)	453, 778, 1072, 1497
MlyI	GAGTC	3 cut(s)	233, 1078, 1142
Mph1103I	ATGCAT	1 cut(s)	421
MroXI	GAANNNNTTC	2 cut(s)	984, 1596
MseI	TTAA	3 cut(s)	455, 489, 794
MslI	CAYNNNNRTG	6 cut(s)	11, 188, 685, 1053, 1620, 1772
MspI	CCGG	3 cut(s)	151, 302, 746
MspR9I	CCNGG	7 cut(s)	152, 266, 302, 746, 747, 969, 1727
MunI	CAATTG	1 cut(s)	1539
MvaI	CCWGG	3 cut(s)	266, 969, 1727
MvnI	CGCG	2 cut(s)	732, 1605
MwoI	GCNNNNNNNGC	7 cut(s)	350, 435, 491, 510, 704, 1519, 1673
NciI	CCSGG	4 cut(s)	152, 302, 746, 747
NcoI	CCATGG	3 cut(s)	189, 219, 1243
NdeII	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
NlaIII	CATG	9 cut(s)	10, 193, 223, 808, 893, 1177, 1247, 1263, 1619
NlaIV	GGNNCC	2 cut(s)	382, 1462
NmeAIII	GCCGAG	1 cut(s)	1297
NmuCI	GTSAC	3 cut(s)	181, 971, 1765
NruI	TCGCGA	1 cut(s)	732
NsiI	ATGCAT	1 cut(s)	421
NspI	RCATGY	1 cut(s)	893
PaeI	GCATGC	1 cut(s)	893
PaeR7I	CTCGAG	1 cut(s)	539
PagI	TCATGA	1 cut(s)	1173
PceI	AGGCCT	1 cut(s)	997
PciSI	GCTCTTC	1 cut(s)	73
PcsI	WCGNNNNNNNCGW	1 cut(s)	97
PdmI	GAANNNNTTC	2 cut(s)	984, 1596
PfeI	GAWTC	5 cut(s)	102, 243, 467, 1098, 1597
PkrI	GCNGC	2 cut(s)	1047, 1714
PleI	GAGTC	3 cut(s)	232, 1078, 1141
PpsI	GAGTC	3 cut(s)	232, 1078, 1141
PshAI	GACNNNNGTC	1 cut(s)	1088
PshBI	ATTAAT	1 cut(s)	794
PsiI	TTATAA	2 cut(s)	786, 845
Psp124BI	GAGCTC	1 cut(s)	778
Psp6I	CCWGG	3 cut(s)	264, 967, 1725
PspFI	CCCAGC	2 cut(s)	22, 388
PspGI	CCWGG	3 cut(s)	264, 967, 1725
PspN4I	GGNNCC	2 cut(s)	382, 1462
PspPI	GGNCC	9 cut(s)	148, 153, 268, 280, 380, 535, 647, 1319, 1512
PsuI	RGATCY	1 cut(s)	1640
RruI	TCGCGA	1 cut(s)	732
RsaI	GTAC	2 cut(s)	55, 111
RsaNI	GTAC	2 cut(s)	54, 110
RseI	CAYNNNNRTG	6 cut(s)	11, 188, 685, 1053, 1620, 1772
SacI	GAGCTC	1 cut(s)	778
SapI	GCTCTTC	1 cut(s)	73
SaqAI	TTAA	3 cut(s)	455, 489, 794
SatI	GCNGC	2 cut(s)	1046, 1713
Sau3AI	GATC	7 cut(s)	18, 97, 118, 573, 727, 1640, 1786
Sau96I	GGNCC	9 cut(s)	148, 153, 268, 280, 380, 535, 647, 1319, 1512
ScaI	AGTACT	1 cut(s)	111
SchI	GAGTC	3 cut(s)	233, 1078, 1142
ScrFI	CCNGG	7 cut(s)	152, 266, 302, 746, 747, 969, 1727
SduI	GDGCHC	4 cut(s)	453, 778, 1072, 1497
SexAI	ACCWGGT	1 cut(s)	1725
SfaNI	GCATC	4 cut(s)	406, 1057, 1102, 1788
SfcI	CTRYAG	2 cut(s)	591, 1580
Sfr274I	CTCGAG	1 cut(s)	539
SinI	GGWCC	4 cut(s)	148, 380, 647, 1319
SlaI	CTCGAG	1 cut(s)	539
SmaI	CCCGGG	1 cut(s)	747
SmiMI	CAYNNNNRTG	6 cut(s)	11, 188, 685, 1053, 1620, 1772
SmlI	CTYRAG	4 cut(s)	347, 539, 1154, 1668
SmoI	CTYRAG	4 cut(s)	347, 539, 1154, 1668
SphI	GCATGC	1 cut(s)	893
SseBI	AGGCCT	1 cut(s)	997
SspI	AATATT	1 cut(s)	818
SspMI	CTAG	3 cut(s)	1073, 1694, 1743
SstI	GAGCTC	1 cut(s)	778
StuI	AGGCCT	1 cut(s)	997
StyD4I	CCNGG	7 cut(s)	150, 264, 300, 744, 745, 967, 1725
StyI	CCWWGG	3 cut(s)	189, 219, 1243
TaaI	ACNGT	5 cut(s)	501, 610, 965, 977, 1771
TaiI	ACGT	1 cut(s)	1534
TaqI	TCGA	7 cut(s)	91, 100, 540, 988, 1051, 1167, 1782
TatI	WGTACW	1 cut(s)	109
TfiI	GAWTC	5 cut(s)	102, 243, 467, 1098, 1597
Tru1I	TTAA	3 cut(s)	455, 489, 794
Tru9I	TTAA	3 cut(s)	455, 489, 794
TscAI	CASTG	3 cut(s)	506, 980, 1373
TseFI	GTSAC	3 cut(s)	181, 971, 1765
TseI	GCWGC	2 cut(s)	1045, 1712
Tsp45I	GTSAC	3 cut(s)	181, 971, 1765
TspGWI	ACGGA	4 cut(s)	130, 217, 1540, 1633
TspMI	CCCGGG	1 cut(s)	745
TspRI	CASTG	3 cut(s)	506, 980, 1373
VneI	GTGCAC	1 cut(s)	1068
VpaK11BI	GGWCC	4 cut(s)	148, 380, 647, 1319
VspI	ATTAAT	1 cut(s)	794
XagI	CCTNNNNNAGG	1 cut(s)	712
XapI	RAATTY	4 cut(s)	305, 560, 1034, 1361
XceI	RCATGY	1 cut(s)	893
XhoI	CTCGAG	1 cut(s)	539
XmaI	CCCGGG	1 cut(s)	745
XmiI	GTMKAC	3 cut(s)	612, 1181, 1483
XmnI	GAANNNNTTC	2 cut(s)	984, 1596
XspI	CTAG	3 cut(s)	1073, 1694, 1743
ZrmI	AGTACT	1 cut(s)	111
Zsp2I	ATGCAT	1 cut(s)	421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.