threonine-tRNA ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 410 |
| AccB1I | GGYRCC | 3 cut(s) | 830, 968, 1690 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 895, 1270 |
| AccBSI | CCGCTC | 1 cut(s) | 1542 |
| AccI | GTMKAC | 1 cut(s) | 1107 |
| AccII | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| AclWI | GGATC | 4 cut(s) | 1016, 1070, 1083, 1270 |
| AcoI | YGGCCR | 2 cut(s) | 570, 720 |
| AcsI | RAATTY | 1 cut(s) | 1636 |
| AcuI | CTGAAG | 2 cut(s) | 1307, 1751 |
| AcvI | CACGTG | 2 cut(s) | 309, 1582 |
| AcyI | GRCGYC | 1 cut(s) | 969 |
| AfaI | GTAC | 6 cut(s) | 452, 731, 770, 887, 1256, 1262 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 134, 398, 572, 895, 965, 971, 1270, 1700, 1725 |
| AgsI | TTSAA | 4 cut(s) | 257, 339, 385, 1501 |
| AjiI | CACGTC | 1 cut(s) | 1270 |
| AjnI | CCWGG | 4 cut(s) | 413, 516, 1263, 1483 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1607, 1639 |
| AluBI | AGCT | 6 cut(s) | 388, 481, 670, 808, 1055, 1741 |
| AluI | AGCT | 6 cut(s) | 388, 481, 670, 808, 1055, 1741 |
| Alw21I | GWGCWC | 2 cut(s) | 483, 1581 |
| Alw26I | GTCTC | 2 cut(s) | 904, 1376 |
| Alw44I | GTGCAC | 1 cut(s) | 1577 |
| AlwI | GGATC | 4 cut(s) | 1016, 1070, 1083, 1270 |
| Ama87I | CYCGRG | 2 cut(s) | 397, 1250 |
| AoxI | GGCC | 9 cut(s) | 471, 489, 519, 528, 570, 720, 906, 1406, 1709 |
| ApaI | GGGCCC | 1 cut(s) | 493 |
| ApaLI | GTGCAC | 1 cut(s) | 1577 |
| ApeKI | GCWGC | 1 cut(s) | 351 |
| ApoI | RAATTY | 1 cut(s) | 1636 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 582, 614, 671, 703 |
| AspLEI | GCGC | 5 cut(s) | 411, 793, 971, 1135, 1405 |
| AspS9I | GGNCC | 7 cut(s) | 314, 401, 489, 490, 520, 535, 1709 |
| AsuC2I | CCSGG | 6 cut(s) | 398, 399, 574, 966, 1069, 1567 |
| AsuHPI | GGTGA | 8 cut(s) | 868, 908, 944, 1085, 1144, 1474, 1693, 1708 |
| AvaI | CYCGRG | 2 cut(s) | 397, 1250 |
| AvaII | GGWCC | 3 cut(s) | 314, 401, 535 |
| BaeGI | GKGCMC | 2 cut(s) | 493, 1581 |
| BamHI | GGATCC | 1 cut(s) | 1075 |
| BanI | GGYRCC | 3 cut(s) | 830, 968, 1690 |
| BanII | GRGCYC | 3 cut(s) | 483, 493, 939 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 629, 661 |
| BbrPI | CACGTG | 2 cut(s) | 309, 1582 |
| BbsI | GAAGAC | 2 cut(s) | 102, 507 |
| Bbv12I | GWGCWC | 2 cut(s) | 483, 1581 |
| BbvI | GCAGC | 1 cut(s) | 338 |
| BccI | CCATC | 3 cut(s) | 74, 326, 1014 |
| BceAI | ACGGC | 5 cut(s) | 256, 458, 721, 1317, 1446 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 400, 434, 655, 689, 1293, 1327 |
| BciT130I | CCWGG | 4 cut(s) | 415, 518, 1265, 1485 |
| BciVI | GTATCC | 1 cut(s) | 573 |
| BclI | TGATCA | 1 cut(s) | 1464 |
| BcnI | CCSGG | 6 cut(s) | 398, 399, 574, 966, 1069, 1567 |
| BcoDI | GTCTC | 2 cut(s) | 904, 1376 |
| BfaI | CTAG | 2 cut(s) | 812, 858 |
| BfmI | CTRYAG | 1 cut(s) | 940 |
| BfoI | RGCGCY | 1 cut(s) | 972 |
| BfuI | GTATCC | 1 cut(s) | 573 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 527 |
| BisI | GCNGC | 5 cut(s) | 279, 352, 789, 1406, 1586 |
| BlpI | GCTNAGC | 1 cut(s) | 1538 |
| BlsI | GCNGC | 5 cut(s) | 280, 353, 790, 1407, 1587 |
| Bme18I | GGWCC | 3 cut(s) | 314, 401, 535 |
| BmeT110I | CYCGRG | 2 cut(s) | 397, 1250 |
| BmgBI | CACGTC | 1 cut(s) | 1270 |
| BmgT120I | GGNCC | 7 cut(s) | 314, 401, 489, 490, 520, 535, 1709 |
| BmiI | GGNNCC | 6 cut(s) | 402, 491, 832, 970, 1077, 1692 |
| BmrI | ACTGGG | 1 cut(s) | 718 |
| BmsI | GCATC | 3 cut(s) | 169, 802, 1516 |
| BmuI | ACTGGG | 1 cut(s) | 718 |
| BoxI | GACNNNNGTC | 1 cut(s) | 399 |
| BpiI | GAAGAC | 2 cut(s) | 102, 507 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1063, 1095 |
| Bpu10I | CCTNAGC | 1 cut(s) | 129 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1538 |
| BpuEI | CTTGAG | 1 cut(s) | 1639 |
| BpuMI | CCSGG | 6 cut(s) | 398, 399, 574, 966, 1069, 1567 |
| Bsa29I | ATCGAT | 1 cut(s) | 345 |
| BsaAI | YACGTR | 2 cut(s) | 309, 1582 |
| BsaHI | GRCGYC | 1 cut(s) | 969 |
| BsaI | GGTCTC | 1 cut(s) | 904 |
| BsaJI | CCNNGG | 6 cut(s) | 397, 531, 964, 1280, 1344, 1483 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 743, 773, 1265, 1295, 1442, 1472 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 134, 398, 572, 895, 965, 971, 1270, 1700, 1725 |
| Bse118I | RCCGGY | 2 cut(s) | 722, 947 |
| Bse1I | ACTGG | 1 cut(s) | 713 |
| Bse3DI | GCAATG | 1 cut(s) | 404 |
| BseBI | CCWGG | 4 cut(s) | 415, 518, 1265, 1485 |
| BseCI | ATCGAT | 1 cut(s) | 345 |
| BseDI | CCNNGG | 6 cut(s) | 397, 531, 964, 1280, 1344, 1483 |
| BseGI | GGATG | 3 cut(s) | 436, 1002, 1537 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 134, 398, 572, 895, 965, 971, 1270, 1700, 1725 |
| BseMI | GCAATG | 1 cut(s) | 404 |
| BseMII | CTCAG | 3 cut(s) | 1043, 1077, 1529 |
| BseNI | ACTGG | 1 cut(s) | 713 |
| BseRI | GAGGAG | 4 cut(s) | 751, 1463, 1466, 1676 |
| BseSI | GKGCMC | 2 cut(s) | 493, 1581 |
| BseX3I | CGGCCG | 1 cut(s) | 720 |
| BseXI | GCAGC | 1 cut(s) | 338 |
| BseYI | CCCAGC | 1 cut(s) | 522 |
| Bsh1236I | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| Bsh1285I | CGRYCG | 1 cut(s) | 723 |
| BshFI | GGCC | 9 cut(s) | 473, 491, 521, 530, 572, 722, 908, 1408, 1711 |
| BshNI | GGYRCC | 3 cut(s) | 830, 968, 1690 |
| BshVI | ATCGAT | 1 cut(s) | 345 |
| BsiEI | CGRYCG | 1 cut(s) | 723 |
| BsiHKAI | GWGCWC | 2 cut(s) | 483, 1581 |
| BsiHKCI | CYCGRG | 2 cut(s) | 397, 1250 |
| BslFI | GGGAC | 2 cut(s) | 725, 1156 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 134, 398, 572, 895, 965, 971, 1270, 1700, 1725 |
| BsmAI | GTCTC | 2 cut(s) | 904, 1376 |
| BsmFI | GGGAC | 2 cut(s) | 725, 1156 |
| BsnI | GGCC | 9 cut(s) | 473, 491, 521, 530, 572, 722, 908, 1408, 1711 |
| Bso31I | GGTCTC | 1 cut(s) | 904 |
| BsoBI | CYCGRG | 2 cut(s) | 397, 1250 |
| Bsp120I | GGGCCC | 1 cut(s) | 489 |
| Bsp1286I | GDGCHC | 4 cut(s) | 483, 493, 939, 1581 |
| Bsp1407I | TGTACA | 1 cut(s) | 729 |
| Bsp143I | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1538 |
| Bsp19I | CCATGG | 1 cut(s) | 531 |
| BspANI | GGCC | 9 cut(s) | 473, 491, 521, 530, 572, 722, 908, 1408, 1711 |
| BspCNI | CTCAG | 3 cut(s) | 1042, 1076, 1530 |
| BspDI | ATCGAT | 1 cut(s) | 345 |
| BspFNI | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| BspLI | GGNNCC | 6 cut(s) | 402, 491, 832, 970, 1077, 1692 |
| BspPI | GGATC | 4 cut(s) | 1016, 1070, 1083, 1270 |
| BspQI | GCTCTTC | 2 cut(s) | 620, 699 |
| BspT107I | GGYRCC | 3 cut(s) | 830, 968, 1690 |
| BspTNI | GGTCTC | 1 cut(s) | 904 |
| BsrBI | CCGCTC | 1 cut(s) | 1542 |
| BsrDI | GCAATG | 1 cut(s) | 404 |
| BsrFI | RCCGGY | 2 cut(s) | 722, 947 |
| BsrGI | TGTACA | 1 cut(s) | 729 |
| BsrI | ACTGG | 1 cut(s) | 713 |
| BssAI | RCCGGY | 2 cut(s) | 722, 947 |
| BssECI | CCNNGG | 6 cut(s) | 397, 531, 964, 1280, 1344, 1483 |
| BssMI | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| BssNI | GRCGYC | 1 cut(s) | 969 |
| BssT1I | CCWWGG | 1 cut(s) | 531 |
| Bst2UI | CCWGG | 4 cut(s) | 415, 518, 1265, 1485 |
| Bst4CI | ACNGT | 6 cut(s) | 247, 542, 557, 632, 944, 1336 |
| Bst6I | CTCTTC | 5 cut(s) | 89, 620, 699, 929, 1415 |
| BstACI | GRCGYC | 1 cut(s) | 969 |
| BstAUI | TGTACA | 1 cut(s) | 729 |
| BstBAI | YACGTR | 2 cut(s) | 309, 1582 |
| BstC8I | GCNNGC | 9 cut(s) | 229, 233, 356, 420, 489, 528, 668, 949, 1644 |
| BstDEI | CTNAG | 6 cut(s) | 129, 301, 903, 1029, 1063, 1538 |
| BstDSI | CCRYGG | 3 cut(s) | 531, 1280, 1344 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 132 |
| BstF5I | GGATG | 3 cut(s) | 436, 1002, 1537 |
| BstFNI | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| BstH2I | RGCGCY | 1 cut(s) | 972 |
| BstHHI | GCGC | 5 cut(s) | 411, 793, 971, 1135, 1405 |
| BstKTI | GATC | 6 cut(s) | 660, 1024, 1078, 1278, 1467, 1737 |
| BstMAI | GTCTC | 2 cut(s) | 904, 1376 |
| BstMBI | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| BstMCI | CGRYCG | 1 cut(s) | 723 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 144, 278, 415, 527, 1439, 1562, 1585 |
| BstNI | CCWGG | 4 cut(s) | 415, 518, 1265, 1485 |
| BstNSI | RCATGY | 2 cut(s) | 736, 1117 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 399 |
| BstSFI | CTRYAG | 1 cut(s) | 940 |
| BstSLI | GKGCMC | 2 cut(s) | 493, 1581 |
| BstUI | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| BstV1I | GCAGC | 1 cut(s) | 338 |
| BstV2I | GAAGAC | 2 cut(s) | 102, 507 |
| BstX2I | RGATCY | 3 cut(s) | 1021, 1075, 1275 |
| BstYI | RGATCY | 3 cut(s) | 1021, 1075, 1275 |
| BstZI | CGGCCG | 1 cut(s) | 720 |
| Bsu15I | ATCGAT | 1 cut(s) | 345 |
| BsuI | GTATCC | 1 cut(s) | 573 |
| BsuRI | GGCC | 9 cut(s) | 473, 491, 521, 530, 572, 722, 908, 1408, 1711 |
| BsuTUI | ATCGAT | 1 cut(s) | 345 |
| BtgI | CCRYGG | 3 cut(s) | 531, 1280, 1344 |
| BtrI | CACGTC | 1 cut(s) | 1270 |
| BtsCI | GGATG | 3 cut(s) | 436, 1002, 1537 |
| BtsI | GCAGTG | 1 cut(s) | 24 |
| BtsIMutI | CAGTG | 4 cut(s) | 24, 538, 949, 1474 |
| Cac8I | GCNNGC | 9 cut(s) | 229, 233, 356, 420, 489, 528, 668, 949, 1644 |
| CfoI | GCGC | 5 cut(s) | 411, 793, 971, 1135, 1405 |
| Cfr10I | RCCGGY | 2 cut(s) | 722, 947 |
| Cfr13I | GGNCC | 7 cut(s) | 314, 401, 489, 490, 520, 535, 1709 |
| Cfr42I | CCGCGG | 1 cut(s) | 1347 |
| Cfr9I | CCCGGG | 1 cut(s) | 397 |
| ClaI | ATCGAT | 1 cut(s) | 345 |
| CpoI | CGGWCCG | 1 cut(s) | 314 |
| CseI | GACGC | 1 cut(s) | 722 |
| Csp6I | GTAC | 6 cut(s) | 451, 730, 769, 886, 1255, 1261 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 358, 393, 590, 625, 808, 843 |
| CspI | CGGWCCG | 1 cut(s) | 314 |
| CviAII | CATG | 8 cut(s) | 484, 532, 661, 733, 883, 1048, 1097, 1114 |
| CviQI | GTAC | 6 cut(s) | 451, 730, 769, 886, 1255, 1261 |
| DdeI | CTNAG | 6 cut(s) | 129, 301, 903, 1029, 1063, 1538 |
| DinI | GGCGCC | 1 cut(s) | 970 |
| DpnI | GATC | 6 cut(s) | 659, 1023, 1077, 1277, 1466, 1736 |
| DpnII | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| EaeI | YGGCCR | 2 cut(s) | 570, 720 |
| EagI | CGGCCG | 1 cut(s) | 720 |
| Eam1104I | CTCTTC | 5 cut(s) | 89, 620, 699, 929, 1415 |
| EarI | CTCTTC | 5 cut(s) | 89, 620, 699, 929, 1415 |
| EciI | GGCGGA | 5 cut(s) | 335, 1448, 1571, 1661, 1673 |
| Ecl136II | GAGCTC | 1 cut(s) | 481 |
| EclXI | CGGCCG | 1 cut(s) | 720 |
| Eco130I | CCWWGG | 1 cut(s) | 531 |
| Eco24I | GRGCYC | 3 cut(s) | 483, 493, 939 |
| Eco31I | GGTCTC | 1 cut(s) | 904 |
| Eco32I | GATATC | 1 cut(s) | 1150 |
| Eco47I | GGWCC | 3 cut(s) | 314, 401, 535 |
| Eco52I | CGGCCG | 1 cut(s) | 720 |
| Eco53kI | GAGCTC | 1 cut(s) | 481 |
| Eco57I | CTGAAG | 2 cut(s) | 1307, 1751 |
| Eco72I | CACGTG | 2 cut(s) | 309, 1582 |
| Eco88I | CYCGRG | 2 cut(s) | 397, 1250 |
| EcoICRI | GAGCTC | 1 cut(s) | 481 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 132 |
| EcoO109I | RGGNCCY | 1 cut(s) | 490 |
| EcoRII | CCWGG | 4 cut(s) | 413, 516, 1263, 1483 |
| EcoRV | GATATC | 1 cut(s) | 1150 |
| EcoT14I | CCWWGG | 1 cut(s) | 531 |
| EcoT38I | GRGCYC | 3 cut(s) | 483, 493, 939 |
| EgeI | GGCGCC | 1 cut(s) | 970 |
| EheI | GGCGCC | 1 cut(s) | 970 |
| ErhI | CCWWGG | 1 cut(s) | 531 |
| FaeI | CATG | 8 cut(s) | 487, 535, 664, 736, 886, 1051, 1100, 1117 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 98, 130, 122, 154, 1601, 1633 |
| FaqI | GGGAC | 2 cut(s) | 725, 1156 |
| FatI | CATG | 8 cut(s) | 483, 531, 660, 732, 882, 1047, 1096, 1113 |
| FauI | CCCGC | 4 cut(s) | 224, 363, 480, 1535 |
| FbaI | TGATCA | 1 cut(s) | 1464 |
| FblI | GTMKAC | 1 cut(s) | 1107 |
| Fnu4HI | GCNGC | 5 cut(s) | 279, 352, 789, 1406, 1586 |
| FokI | GGATG | 3 cut(s) | 443, 989, 1544 |
| FriOI | GRGCYC | 3 cut(s) | 483, 493, 939 |
| Fsp4HI | GCNGC | 5 cut(s) | 279, 352, 789, 1406, 1586 |
| FspBI | CTAG | 2 cut(s) | 812, 858 |
| FspI | TGCGCA | 1 cut(s) | 410 |
| GlaI | GCGC | 5 cut(s) | 410, 792, 970, 1134, 1404 |
| GluI | GCNGC | 5 cut(s) | 279, 352, 789, 1406, 1586 |
| GsaI | CCCAGC | 1 cut(s) | 526 |
| HaeII | RGCGCY | 1 cut(s) | 972 |
| HaeIII | GGCC | 9 cut(s) | 473, 491, 521, 530, 572, 722, 908, 1408, 1711 |
| HgaI | GACGC | 1 cut(s) | 722 |
| HhaI | GCGC | 5 cut(s) | 411, 793, 971, 1135, 1405 |
| Hin1I | GRCGYC | 1 cut(s) | 969 |
| Hin1II | CATG | 8 cut(s) | 487, 535, 664, 736, 886, 1051, 1100, 1117 |
| Hin6I | GCGC | 5 cut(s) | 409, 791, 969, 1133, 1403 |
| HinP1I | GCGC | 5 cut(s) | 409, 791, 969, 1133, 1403 |
| HincII | GTYRAC | 1 cut(s) | 1632 |
| HindII | GTYRAC | 1 cut(s) | 1632 |
| HindIII | AAGCTT | 1 cut(s) | 806 |
| HinfI | GANTC | 5 cut(s) | 861, 1204, 1215, 1229, 1241 |
| HpaI | GTTAAC | 1 cut(s) | 1632 |
| HphI | GGTGA | 8 cut(s) | 868, 908, 944, 1085, 1144, 1474, 1693, 1708 |
| Hpy166II | GTNNAC | 6 cut(s) | 451, 538, 601, 1108, 1579, 1632 |
| Hpy188I | TCNGA | 3 cut(s) | 583, 1032, 1214 |
| Hpy188III | TCNNGA | 9 cut(s) | 176, 254, 428, 497, 703, 812, 982, 1017, 1609 |
| Hpy8I | GTNNAC | 6 cut(s) | 451, 538, 601, 1108, 1579, 1632 |
| Hpy99I | CGWCG | 7 cut(s) | 479, 557, 680, 956, 1196, 1336, 1375 |
| HpyAV | CCTTC | 6 cut(s) | 503, 1321, 1419, 1432, 1540, 1700 |
| HpyCH4III | ACNGT | 6 cut(s) | 247, 542, 557, 632, 944, 1336 |
| HpyCH4IV | ACGT | 6 cut(s) | 308, 1104, 1269, 1373, 1581, 1685 |
| HpyCH4V | TGCA | 3 cut(s) | 147, 272, 1579 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 144, 278, 415, 527, 1439, 1562, 1585 |
| HpyF3I | CTNAG | 6 cut(s) | 129, 301, 903, 1029, 1063, 1538 |
| HpySE526I | ACGT | 6 cut(s) | 308, 1104, 1269, 1373, 1581, 1685 |
| Hsp92I | GRCGYC | 1 cut(s) | 969 |
| Hsp92II | CATG | 8 cut(s) | 487, 535, 664, 736, 886, 1051, 1100, 1117 |
| HspAI | GCGC | 5 cut(s) | 409, 791, 969, 1133, 1403 |
| KasI | GGCGCC | 1 cut(s) | 968 |
| KroI | GCCGGC | 1 cut(s) | 947 |
| KroNI | GCCGGC | 1 cut(s) | 949 |
| Ksp22I | TGATCA | 1 cut(s) | 1464 |
| KspAI | GTTAAC | 1 cut(s) | 1632 |
| KspI | CCGCGG | 1 cut(s) | 1347 |
| Kzo9I | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| LguI | GCTCTTC | 2 cut(s) | 620, 699 |
| LmnI | GCTCC | 2 cut(s) | 721, 1052 |
| Lsp1109I | GCAGC | 1 cut(s) | 338 |
| LweI | GCATC | 3 cut(s) | 169, 802, 1516 |
| MaeI | CTAG | 2 cut(s) | 812, 858 |
| MaeII | ACGT | 6 cut(s) | 308, 1104, 1269, 1373, 1581, 1685 |
| MaeIII | GTNAC | 2 cut(s) | 1226, 1681 |
| MalI | GATC | 6 cut(s) | 659, 1023, 1077, 1277, 1466, 1736 |
| MbiI | CCGCTC | 1 cut(s) | 1542 |
| MboI | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| MflI | RGATCY | 3 cut(s) | 1021, 1075, 1275 |
| MhlI | GDGCHC | 4 cut(s) | 483, 493, 939, 1581 |
| MluCI | AATT | 4 cut(s) | 117, 191, 820, 1636 |
| Mly113I | GGCGCC | 1 cut(s) | 969 |
| MlyI | GAGTC | 3 cut(s) | 1223, 1224, 1250 |
| MmeI | TCCRAC | 1 cut(s) | 606 |
| MroNI | GCCGGC | 1 cut(s) | 947 |
| MseI | TTAA | 3 cut(s) | 116, 1170, 1631 |
| MslI | CAYNNNNRTG | 1 cut(s) | 881 |
| MspA1I | CMGCKG | 1 cut(s) | 1346 |
| MvaI | CCWGG | 4 cut(s) | 415, 518, 1265, 1485 |
| MvnI | CGCG | 4 cut(s) | 791, 1135, 1346, 1405 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 144, 278, 415, 527, 1439, 1562, 1585 |
| NaeI | GCCGGC | 1 cut(s) | 949 |
| NarI | GGCGCC | 1 cut(s) | 969 |
| NciI | CCSGG | 6 cut(s) | 398, 399, 574, 966, 1069, 1567 |
| NcoI | CCATGG | 1 cut(s) | 531 |
| NdeII | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| NgoMIV | GCCGGC | 1 cut(s) | 947 |
| NlaIII | CATG | 8 cut(s) | 487, 535, 664, 736, 886, 1051, 1100, 1117 |
| NlaIV | GGNNCC | 6 cut(s) | 402, 491, 832, 970, 1077, 1692 |
| NmuCI | GTSAC | 2 cut(s) | 1226, 1681 |
| NsbI | TGCGCA | 1 cut(s) | 410 |
| NspI | RCATGY | 2 cut(s) | 736, 1117 |
| PciSI | GCTCTTC | 2 cut(s) | 620, 699 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1691 |
| PdiI | GCCGGC | 1 cut(s) | 949 |
| PfeI | GAWTC | 2 cut(s) | 861, 1204 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 895, 1270 |
| PkrI | GCNGC | 5 cut(s) | 280, 353, 790, 1407, 1587 |
| PleI | GAGTC | 3 cut(s) | 1223, 1223, 1249 |
| PluTI | GGCGCC | 1 cut(s) | 972 |
| PmaCI | CACGTG | 2 cut(s) | 309, 1582 |
| PmlI | CACGTG | 2 cut(s) | 309, 1582 |
| PpsI | GAGTC | 3 cut(s) | 1223, 1223, 1249 |
| Ppu21I | YACGTR | 2 cut(s) | 309, 1582 |
| PshAI | GACNNNNGTC | 1 cut(s) | 399 |
| Psp124BI | GAGCTC | 1 cut(s) | 483 |
| Psp6I | CCWGG | 4 cut(s) | 413, 516, 1263, 1483 |
| PspCI | CACGTG | 2 cut(s) | 309, 1582 |
| PspFI | CCCAGC | 1 cut(s) | 522 |
| PspGI | CCWGG | 4 cut(s) | 413, 516, 1263, 1483 |
| PspN4I | GGNNCC | 6 cut(s) | 402, 491, 832, 970, 1077, 1692 |
| PspOMI | GGGCCC | 1 cut(s) | 489 |
| PspPI | GGNCC | 7 cut(s) | 314, 401, 489, 490, 520, 535, 1709 |
| PsuI | RGATCY | 3 cut(s) | 1021, 1075, 1275 |
| RsaI | GTAC | 6 cut(s) | 452, 731, 770, 887, 1256, 1262 |
| RsaNI | GTAC | 6 cut(s) | 451, 730, 769, 886, 1255, 1261 |
| RseI | CAYNNNNRTG | 1 cut(s) | 881 |
| Rsr2I | CGGWCCG | 1 cut(s) | 314 |
| RsrII | CGGWCCG | 1 cut(s) | 314 |
| SacI | GAGCTC | 1 cut(s) | 483 |
| SacII | CCGCGG | 1 cut(s) | 1347 |
| SapI | GCTCTTC | 2 cut(s) | 620, 699 |
| SaqAI | TTAA | 3 cut(s) | 116, 1170, 1631 |
| SatI | GCNGC | 5 cut(s) | 279, 352, 789, 1406, 1586 |
| Sau3AI | GATC | 6 cut(s) | 657, 1021, 1075, 1275, 1464, 1734 |
| Sau96I | GGNCC | 7 cut(s) | 314, 401, 489, 490, 520, 535, 1709 |
| SchI | GAGTC | 3 cut(s) | 1223, 1224, 1250 |
| SduI | GDGCHC | 4 cut(s) | 483, 493, 939, 1581 |
| SfaNI | GCATC | 3 cut(s) | 169, 802, 1516 |
| SfcI | CTRYAG | 1 cut(s) | 940 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 527 |
| SfoI | GGCGCC | 1 cut(s) | 970 |
| Sfr303I | CCGCGG | 1 cut(s) | 1347 |
| SgrBI | CCGCGG | 1 cut(s) | 1347 |
| SinI | GGWCC | 3 cut(s) | 314, 401, 535 |
| SmaI | CCCGGG | 1 cut(s) | 399 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 881 |
| SmlI | CTYRAG | 1 cut(s) | 1618 |
| SmoI | CTYRAG | 1 cut(s) | 1618 |
| Sse9I | AATT | 4 cut(s) | 117, 191, 820, 1636 |
| SspDI | GGCGCC | 1 cut(s) | 968 |
| SspMI | CTAG | 2 cut(s) | 812, 858 |
| SstI | GAGCTC | 1 cut(s) | 483 |
| StyI | CCWWGG | 1 cut(s) | 531 |
| TaaI | ACNGT | 6 cut(s) | 247, 542, 557, 632, 944, 1336 |
| TaiI | ACGT | 6 cut(s) | 311, 1107, 1272, 1376, 1584, 1688 |
| TasI | AATT | 4 cut(s) | 117, 191, 820, 1636 |
| TatI | WGTACW | 2 cut(s) | 450, 729 |
| TauI | GCSGC | 4 cut(s) | 281, 791, 1408, 1588 |
| TfiI | GAWTC | 2 cut(s) | 861, 1204 |
| Tru1I | TTAA | 3 cut(s) | 116, 1170, 1631 |
| Tru9I | TTAA | 3 cut(s) | 116, 1170, 1631 |
| TscAI | CASTG | 4 cut(s) | 31, 545, 949, 1474 |
| TseFI | GTSAC | 2 cut(s) | 1226, 1681 |
| TseI | GCWGC | 1 cut(s) | 351 |
| Tsp45I | GTSAC | 2 cut(s) | 1226, 1681 |
| TspGWI | ACGGA | 2 cut(s) | 255, 1214 |
| TspMI | CCCGGG | 1 cut(s) | 397 |
| TspRI | CASTG | 4 cut(s) | 31, 545, 949, 1474 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 895, 1270 |
| VneI | GTGCAC | 1 cut(s) | 1577 |
| VpaK11BI | GGWCC | 3 cut(s) | 314, 401, 535 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 132 |
| XapI | RAATTY | 1 cut(s) | 1636 |
| XbaI | TCTAGA | 1 cut(s) | 811 |
| XceI | RCATGY | 2 cut(s) | 736, 1117 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 705 |
| XmaI | CCCGGG | 1 cut(s) | 397 |
| XmiI | GTMKAC | 1 cut(s) | 1107 |
| XspI | CTAG | 2 cut(s) | 812, 858 |