Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Reverse (-)

6156057 .. 6162258

Gene Structure 6202 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1089100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2031 bp

ATGGTGCCCCATCCGAAAGACGACGCGTATCTCGAGGCCGTAATCCCGAAGCGCATTAAGCTCTTCGAGGCCATACAGGCCCAGCAGCTCGCCCACCGCCAGTCCCTCCCCTCCGATCCCATCAAGATTACACTGCCGGGTGGACAGGAGAAGGAAGGGAAGAGGTGGGAGACTTCGCCATTCGACATTGCCAAGGATATTTCCAAGAGCTTGGCTTCCAGCGCTGTGATTGCGAAGGTCAATGGGGACCTTTGGGACATGGCCAGGCCGCTGGAGTTTGACTGTGAGCTCAAGCTCTTCAAATTTGAAGACGATGAAGGACGTGATACTTTCTGGCATTCCAGCGCTCACATTCTTGGACAGGCACTTGAGGTTGAGTATGGATGCAAGCTCTGCATTGGTCCGTGCACTACAAGAGGAGAGGGTTTCTATTACGATGCTTTCTATGGTGATTTGGGGTTGAATGATGAACACTTCAAGGACATTGCATCAGCGGCAGATAAGGCTGTAAAGGCTAAACAGCCTTTTGAGCGTATCGAAGTAACACGTGAACAGGCACTTGAGATATTTTCTGATAATCAATTCAAGGTTGAAATCATAAATGATCTTCCGGCAGACAAAACTATCACAGTATATAGATGTGGCCCCTTGGTTGACTTGTGCCGTGGACCCCATATACCAAATACATCCTTTGTCAAAGCATTTGCATGTTTGAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGTGAAAGTTTACAGAGAGTTTATGGAATATCATATCCTGATAAGAAAAGTTTGCAGATATACATCCAGAAGCTGGAAGAAGCAAAAAAGTATGACCACAGACTGTTGGGTACAAAGCAGGAGCTCTTTTTTTGTCACCCACTTAGTCCAGGAAGTTGGTTCTTCCTCCCTAATGGGACTCGGATTTATAACAAACTAATGGAGTTCATAAAGAATCAATACAGAGAACGAGGCTATAAAGAGGTTATATCACCAAACATGTACAATATGCAACTTTGGGAAACATCTGGTCATGCTGCAAATTACAAAGAGAATATGTTTGTTTTTGAGATTGAGAAACAAGAATTTGGATTGAAGCCAATGAATTGCCCAGGGCATTGTTTGATGTTTCAGCATAGGGTTCGTTCTTATAGAGAACTTCCACTTAGACTCGCTGATTTTGGTGTTTTGCATCGCAATGAGGCCAGTGGTGCACTGACTGGATTAACACGCGTACGGAGATTCCAGCAGGATGATGCCCATATCTTTTGCAGGGAATCCCAAGTAAAAGATGAAGTGAAGGGTGTCTTGGATTTTATCCAGTATGCCTATAATGTTTTTGGCTTTTCTTTTGAACTGAAGCTATCAACAATGCCAGAAAAACACATGGGAGATTTGGCAACATGGGAGAAAGCTGAGGCTGCCCTTGCAGACGCATTAAATGAGTTTGGCAAGCCCTGGCAGATAAATGAAGGGGATGGTGCTTTTTATGGACCAAAGATAGATATCAGTGTCTCTGATGCATTGAACAGAAAATATCAGTGTGCAACACTACAGCTTGATTTTCAGCTTCCTCAGAAGTTCGAGCTGTATTACTCAGCAGAGGGGGAAGAAGGCAAGTTGGAGAGACCCGTTATGATACACAGAGCTGTTTTAGGTTCTGTTGAGCGTATGTTTGCTATATTGTTGGAGAACTACAAAGGGAAATGGCCCTTCTGGCTTAGTCCACGTCAAGCAATTGTTTGCCCCGTCTCGGACAAATCCCAACCTTACGCTCAGGAGGTTCGGGACCGTATCCACGAAGCTGGTTATTATGTGGATGTTGACACAAGTGATAGAACCATCCAAAAGAAGGTGCGAGAAGCTCAGATAGCGCAGTACAACTACATTTTAGTTGTTGGCGAGGAGGAAGTCAAGACTGGGCAGGTGAGCTTACGAGTTCGAGACCACGGAGACGTCACGGTAATGACAATGGAAAATCTACTCCAGCACTTCAAGGCTGAAGTTGAAGCTTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

677

Amino Acids

77.74

Weight (kDa)

6.08

Isoelectric Point (pI)

33.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	41 - 101	2.4e-16	TGS domain
tRNA_SAD	PF07973	209 - 258	1.7e-13	Threonyl and Alanyl tRNA synthetase second additional domain
tRNA-synt_2b	PF00587	357 - 569	5.6e-46	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	581 - 670	1.2e-21	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	939
AarI	CACCTGC	1 cut(s)	1924
AatII	GACGTC	1 cut(s)	1968
Acc36I	ACCTGC	1 cut(s)	1924
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	823
AccII	CGCG	2 cut(s)	26, 1242
AciI	CCGC	3 cut(s)	97, 269, 494
AclWI	GGATC	1 cut(s)	110
AcoI	YGGCCR	1 cut(s)	261
AcsI	RAATTY	2 cut(s)	302, 1094
AcuI	CTGAAG	3 cut(s)	708, 1388, 2031
AcvI	CACGTG	1 cut(s)	548
AcyI	GRCGYC	1 cut(s)	1965
AfaI	GTAC	4 cut(s)	862, 1013, 1245, 1889
AfeI	AGCGCT	2 cut(s)	223, 346
AfiI	CCNNNNNNNGG	4 cut(s)	735, 823, 1762, 1861
AflIII	ACRYGT	4 cut(s)	24, 545, 1008, 1240
AjiI	CACGTC	2 cut(s)	323, 1739
AjnI	CCWGG	4 cut(s)	263, 898, 1120, 1466
AjuI	GAANNNNNNNTTGG	2 cut(s)	1301, 1333
Alw21I	GWGCWC	4 cut(s)	291, 410, 876, 1225
Alw26I	GTCTC	6 cut(s)	164, 1528, 1630, 1765, 1947, 1956
Alw44I	GTGCAC	2 cut(s)	406, 1221
AlwI	GGATC	1 cut(s)	110
AlwNI	CAGNNNCTG	1 cut(s)	823
Ama87I	CYCGRG	1 cut(s)	32
Aor51HI	AGCGCT	2 cut(s)	223, 346
AoxI	GGCC	8 cut(s)	36, 69, 78, 261, 266, 643, 1212, 1718
ApaLI	GTGCAC	2 cut(s)	406, 1221
ApeKI	GCWGC	3 cut(s)	85, 1046, 1430
ApoI	RAATTY	2 cut(s)	302, 1094
AspLEI	GCGC	4 cut(s)	54, 224, 347, 1885
AspS9I	GGNCC	8 cut(s)	79, 247, 401, 644, 668, 1502, 1719, 1798
AsuC2I	CCSGG	1 cut(s)	138
AsuHPI	GGTGA	4 cut(s)	461, 878, 993, 1948
AvaI	CYCGRG	1 cut(s)	32
AvaII	GGWCC	5 cut(s)	247, 401, 668, 1502, 1798
BaeGI	GKGCMC	3 cut(s)	9, 410, 1225
BalI	TGGCCA	1 cut(s)	263
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	291, 876
BbrPI	CACGTG	1 cut(s)	548
BbsI	GAAGAC	1 cut(s)	315
Bbv12I	GWGCWC	4 cut(s)	291, 410, 876, 1225
BbvCI	CCTCAGC	1 cut(s)	1425
BbvI	GCAGC	3 cut(s)	97, 1033, 1417
BccI	CCATC	4 cut(s)	18, 128, 1481, 1859
BceAI	ACGGC	2 cut(s)	23, 648
BcgI	CGANNNNNNTGC	2 cut(s)	1133, 1167
BciT130I	CCWGG	4 cut(s)	265, 900, 1122, 1468
BciVI	GTATCC	1 cut(s)	1814
BcnI	CCSGG	1 cut(s)	138
BcoDI	GTCTC	6 cut(s)	164, 1528, 1630, 1765, 1947, 1956
BfmI	CTRYAG	1 cut(s)	1562
BfoI	RGCGCY	2 cut(s)	225, 348
BfuAI	ACCTGC	1 cut(s)	1924
BfuI	GTATCC	1 cut(s)	1814
BglI	GCCNNNNNGGC	2 cut(s)	77, 1726
BisI	GCNGC	5 cut(s)	86, 269, 495, 1047, 1431
BlsI	GCNGC	5 cut(s)	87, 270, 496, 1048, 1432
Bme1390I	CCNGG	5 cut(s)	138, 265, 900, 1122, 1468
Bme18I	GGWCC	5 cut(s)	247, 401, 668, 1502, 1798
BmeT110I	CYCGRG	1 cut(s)	32
BmgBI	CACGTC	2 cut(s)	323, 1739
BmgT120I	GGNCC	8 cut(s)	79, 247, 401, 644, 668, 1502, 1719, 1798
BmiI	GGNNCC	5 cut(s)	6, 248, 646, 670, 1799
BmrFI	CCNGG	5 cut(s)	138, 265, 900, 1122, 1468
BmrI	ACTGGG	1 cut(s)	1938
BmsI	GCATC	6 cut(s)	374, 427, 497, 1210, 1255, 1519
BmuI	ACTGGG	1 cut(s)	1938
BpiI	GAAGAC	1 cut(s)	315
BpmI	CTGGAG	3 cut(s)	293, 754, 1979
Bpu10I	CCTNAGC	2 cut(s)	1425, 1785
BpuEI	CTTGAG	3 cut(s)	275, 389, 581
BpuMI	CCSGG	1 cut(s)	138
BsaAI	YACGTR	1 cut(s)	548
BsaBI	GATNNNNATC	2 cut(s)	812, 1514
BsaHI	GRCGYC	1 cut(s)	1965
BsaI	GGTCTC	2 cut(s)	1630, 1947
BsaJI	CCNNGG	7 cut(s)	192, 648, 664, 1120, 1121, 1466, 1957
Bsc4I	CCNNNNNNNGG	4 cut(s)	735, 823, 1762, 1861
Bse1I	ACTGG	6 cut(s)	100, 737, 1215, 1234, 1330, 1933
Bse3DI	GCAATG	3 cut(s)	186, 483, 1213
Bse8I	GATNNNNATC	2 cut(s)	812, 1514
BseBI	CCWGG	4 cut(s)	265, 900, 1122, 1468
BseDI	CCNNGG	7 cut(s)	192, 648, 664, 1120, 1121, 1466, 1957
BseGI	GGATG	8 cut(s)	10, 389, 686, 813, 1267, 1492, 1834, 1851
BseJI	GATNNNNATC	2 cut(s)	812, 1514
BseLI	CCNNNNNNNGG	4 cut(s)	735, 823, 1762, 1861
BseMI	GCAATG	3 cut(s)	186, 483, 1213
BseMII	CTCAG	5 cut(s)	1416, 1598, 1620, 1799, 1889
BseNI	ACTGG	6 cut(s)	100, 737, 1215, 1234, 1330, 1933
BseRI	GAGGAG	2 cut(s)	432, 1928
BseSI	GKGCMC	3 cut(s)	9, 410, 1225
BseXI	GCAGC	3 cut(s)	97, 1033, 1417
BseYI	CCCAGC	1 cut(s)	81
Bsh1236I	CGCG	2 cut(s)	26, 1242
BshFI	GGCC	8 cut(s)	38, 71, 80, 263, 268, 645, 1214, 1720
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	4 cut(s)	291, 410, 876, 1225
BsiHKCI	CYCGRG	1 cut(s)	32
BsiSI	CCGG	2 cut(s)	137, 611
BsiWI	CGTACG	1 cut(s)	1243
BslFI	GGGAC	5 cut(s)	88, 260, 269, 940, 1811
BslI	CCNNNNNNNGG	4 cut(s)	735, 823, 1762, 1861
BsmAI	GTCTC	6 cut(s)	164, 1528, 1630, 1765, 1947, 1956
BsmBI	CGTCTC	2 cut(s)	1765, 1956
BsmFI	GGGAC	5 cut(s)	88, 260, 269, 940, 1811
BsmI	GAATGC	1 cut(s)	337
BsnI	GGCC	8 cut(s)	38, 71, 80, 263, 268, 645, 1214, 1720
Bso31I	GGTCTC	2 cut(s)	1630, 1947
BsoBI	CYCGRG	1 cut(s)	32
Bsp1286I	GDGCHC	5 cut(s)	9, 291, 410, 876, 1225
Bsp1407I	TGTACA	1 cut(s)	1011
Bsp143I	GATC	3 cut(s)	115, 604, 748
BspACI	CCGC	3 cut(s)	97, 269, 494
BspANI	GGCC	8 cut(s)	38, 71, 80, 263, 268, 645, 1214, 1720
BspCNI	CTCAG	5 cut(s)	1417, 1597, 1619, 1798, 1888
BspFNI	CGCG	2 cut(s)	26, 1242
BspLI	GGNNCC	5 cut(s)	6, 248, 646, 670, 1799
BspMI	ACCTGC	1 cut(s)	1924
BspPI	GGATC	1 cut(s)	110
BspQI	GCTCTTC	2 cut(s)	68, 302
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	2 cut(s)	1630, 1947
BsrDI	GCAATG	3 cut(s)	186, 483, 1213
BsrGI	TGTACA	1 cut(s)	1011
BsrI	ACTGG	6 cut(s)	100, 737, 1215, 1234, 1330, 1933
BssECI	CCNNGG	7 cut(s)	192, 648, 664, 1120, 1121, 1466, 1957
BssMI	GATC	3 cut(s)	115, 604, 748
BssNI	GRCGYC	1 cut(s)	1965
BssT1I	CCWWGG	2 cut(s)	192, 648
Bst2UI	CCWGG	4 cut(s)	265, 900, 1122, 1468
Bst4CI	ACNGT	5 cut(s)	284, 631, 855, 1802, 1972
Bst6I	CTCTTC	3 cut(s)	68, 155, 302
BstACI	GRCGYC	1 cut(s)	1965
BstAPI	GCANNNNNTGC	1 cut(s)	393
BstAUI	TGTACA	1 cut(s)	1011
BstBAI	YACGTR	1 cut(s)	548
BstC8I	GCNNGC	3 cut(s)	90, 389, 1463
BstDEI	CTNAG	8 cut(s)	893, 1175, 1425, 1584, 1606, 1730, 1785, 1875
BstDSI	CCRYGG	2 cut(s)	664, 1957
BstENI	CCTNNNNNAGG	1 cut(s)	733
BstF5I	GGATG	8 cut(s)	10, 389, 686, 813, 1267, 1492, 1834, 1851
BstFNI	CGCG	2 cut(s)	26, 1242
BstH2I	RGCGCY	2 cut(s)	225, 348
BstHHI	GCGC	4 cut(s)	54, 224, 347, 1885
BstKTI	GATC	3 cut(s)	118, 607, 751
BstMAI	GTCTC	6 cut(s)	164, 1528, 1630, 1765, 1947, 1956
BstMBI	GATC	3 cut(s)	115, 604, 748
BstNI	CCWGG	4 cut(s)	265, 900, 1122, 1468
BstNSI	RCATGY	2 cut(s)	711, 1012
BstSCI	CCNGG	5 cut(s)	136, 263, 898, 1120, 1466
BstSFI	CTRYAG	1 cut(s)	1562
BstSLI	GKGCMC	3 cut(s)	9, 410, 1225
BstUI	CGCG	2 cut(s)	26, 1242
BstV1I	GCAGC	3 cut(s)	97, 1033, 1417
BstV2I	GAAGAC	1 cut(s)	315
BstXI	CCANNNNNNTGG	4 cut(s)	211, 271, 906, 1814
BsuI	GTATCC	1 cut(s)	1814
BsuRI	GGCC	8 cut(s)	38, 71, 80, 263, 268, 645, 1214, 1720
BtgI	CCRYGG	2 cut(s)	664, 1957
BtgZI	GCGATG	1 cut(s)	1187
BtrI	CACGTC	2 cut(s)	323, 1739
BtsCI	GGATG	8 cut(s)	10, 389, 686, 813, 1267, 1492, 1834, 1851
BtsI	GCAGTG	1 cut(s)	131
BtsIMutI	CAGTG	5 cut(s)	131, 1222, 1223, 1525, 1556
BveI	ACCTGC	1 cut(s)	1924
Cac8I	GCNNGC	3 cut(s)	90, 389, 1463
CaiI	CAGNNNCTG	1 cut(s)	823
CfoI	GCGC	4 cut(s)	54, 224, 347, 1885
Cfr13I	GGNCC	8 cut(s)	79, 247, 401, 644, 668, 1502, 1719, 1798
CseI	GACGC	2 cut(s)	32, 1451
Csp6I	GTAC	4 cut(s)	861, 1012, 1244, 1888
CviAII	CATG	6 cut(s)	259, 708, 1009, 1043, 1396, 1413
CviQI	GTAC	4 cut(s)	861, 1012, 1244, 1888
DdeI	CTNAG	8 cut(s)	893, 1175, 1425, 1584, 1606, 1730, 1785, 1875
DpnI	GATC	3 cut(s)	117, 606, 750
DpnII	GATC	3 cut(s)	115, 604, 748
EaeI	YGGCCR	1 cut(s)	261
Eam1104I	CTCTTC	3 cut(s)	68, 155, 302
EarI	CTCTTC	3 cut(s)	68, 155, 302
Ecl136II	GAGCTC	2 cut(s)	289, 874
Eco130I	CCWWGG	2 cut(s)	192, 648
Eco24I	GRGCYC	2 cut(s)	291, 876
Eco31I	GGTCTC	2 cut(s)	1630, 1947
Eco32I	GATATC	1 cut(s)	1516
Eco47I	GGWCC	5 cut(s)	247, 401, 668, 1502, 1798
Eco47III	AGCGCT	2 cut(s)	223, 346
Eco53kI	GAGCTC	2 cut(s)	289, 874
Eco57I	CTGAAG	3 cut(s)	708, 1388, 2031
Eco72I	CACGTG	1 cut(s)	548
Eco88I	CYCGRG	1 cut(s)	32
EcoICRI	GAGCTC	2 cut(s)	289, 874
EcoNI	CCTNNNNNAGG	1 cut(s)	733
EcoO109I	RGGNCCY	1 cut(s)	247
EcoRII	CCWGG	4 cut(s)	263, 898, 1120, 1466
EcoRV	GATATC	1 cut(s)	1516
EcoT14I	CCWWGG	2 cut(s)	192, 648
EcoT22I	ATGCAT	1 cut(s)	1534
EcoT38I	GRGCYC	2 cut(s)	291, 876
ErhI	CCWWGG	2 cut(s)	192, 648
Esp3I	CGTCTC	2 cut(s)	1765, 1956
FaeI	CATG	6 cut(s)	262, 711, 1012, 1046, 1399, 1416
FalI	AAGNNNNNCTT	2 cut(s)	1301, 1333
FaqI	GGGAC	5 cut(s)	88, 260, 269, 940, 1811
FatI	CATG	6 cut(s)	258, 707, 1008, 1042, 1395, 1412
Fnu4HI	GCNGC	5 cut(s)	86, 269, 495, 1047, 1431
FokI	GGATG	7 cut(s)	396, 673, 800, 1274, 1499, 1838, 1841
FriOI	GRGCYC	2 cut(s)	291, 876
Fsp4HI	GCNGC	5 cut(s)	86, 269, 495, 1047, 1431
GlaI	GCGC	4 cut(s)	53, 223, 346, 1884
GluI	GCNGC	5 cut(s)	86, 269, 495, 1047, 1431
GsaI	CCCAGC	1 cut(s)	85
GsuI	CTGGAG	3 cut(s)	293, 754, 1979
HaeII	RGCGCY	2 cut(s)	225, 348
HaeIII	GGCC	8 cut(s)	38, 71, 80, 263, 268, 645, 1214, 1720
HapII	CCGG	2 cut(s)	137, 611
HgaI	GACGC	2 cut(s)	32, 1451
HhaI	GCGC	4 cut(s)	54, 224, 347, 1885
Hin1I	GRCGYC	1 cut(s)	1965
Hin1II	CATG	6 cut(s)	262, 711, 1012, 1046, 1399, 1416
Hin6I	GCGC	4 cut(s)	52, 222, 345, 1883
HinP1I	GCGC	4 cut(s)	52, 222, 345, 1883
HincII	GTYRAC	2 cut(s)	655, 1834
HindII	GTYRAC	2 cut(s)	655, 1834
HindIII	AAGCTT	1 cut(s)	2019
HinfI	GANTC	5 cut(s)	928, 964, 1179, 1251, 1286
HpaII	CCGG	2 cut(s)	137, 611
HphI	GGTGA	4 cut(s)	461, 878, 993, 1948
Hpy166II	GTNNAC	9 cut(s)	143, 408, 551, 655, 668, 761, 1223, 1736, 1834
Hpy188I	TCNGA	8 cut(s)	15, 115, 574, 933, 1528, 1587, 1765, 1878
Hpy8I	GTNNAC	9 cut(s)	143, 408, 551, 655, 668, 761, 1223, 1736, 1834
Hpy99I	CGWCG	1 cut(s)	26
HpyCH4III	ACNGT	5 cut(s)	284, 631, 855, 1802, 1972
HpyCH4IV	ACGT	4 cut(s)	322, 547, 1738, 1965
HpyF3I	CTNAG	8 cut(s)	893, 1175, 1425, 1584, 1606, 1730, 1785, 1875
HpySE526I	ACGT	4 cut(s)	322, 547, 1738, 1965
Hsp92I	GRCGYC	1 cut(s)	1965
Hsp92II	CATG	6 cut(s)	262, 711, 1012, 1046, 1399, 1416
HspAI	GCGC	4 cut(s)	52, 222, 345, 1883
Kzo9I	GATC	3 cut(s)	115, 604, 748
LguI	GCTCTTC	2 cut(s)	68, 302
LmnI	GCTCC	1 cut(s)	871
Lsp1109I	GCAGC	3 cut(s)	97, 1033, 1417
LweI	GCATC	6 cut(s)	374, 427, 497, 1210, 1255, 1519
MaeII	ACGT	4 cut(s)	322, 547, 1738, 1965
MaeIII	GTNAC	3 cut(s)	541, 884, 1966
MalI	GATC	3 cut(s)	117, 606, 750
MboI	GATC	3 cut(s)	115, 604, 748
MboII	GAAGA	9 cut(s)	55, 172, 289, 320, 599, 714, 839, 904, 1631
MfeI	CAATTG	1 cut(s)	1746
MhlI	GDGCHC	5 cut(s)	9, 291, 410, 876, 1225
MlsI	TGGCCA	1 cut(s)	263
MluCI	AATT	6 cut(s)	302, 581, 1051, 1094, 1114, 1746
MluI	ACGCGT	2 cut(s)	24, 1240
MluNI	TGGCCA	1 cut(s)	263
MlyI	GAGTC	2 cut(s)	922, 1173
MmeI	TCCRAC	2 cut(s)	1611, 1677
Mox20I	TGGCCA	1 cut(s)	263
Mph1103I	ATGCAT	1 cut(s)	1534
MscI	TGGCCA	1 cut(s)	263
MseI	TTAA	3 cut(s)	57, 1235, 1448
MslI	CAYNNNNRTG	3 cut(s)	706, 1206, 1973
Msp20I	TGGCCA	1 cut(s)	263
MspA1I	CMGCKG	2 cut(s)	271, 494
MspI	CCGG	2 cut(s)	137, 611
MspR9I	CCNGG	5 cut(s)	138, 265, 900, 1122, 1468
MunI	CAATTG	1 cut(s)	1746
Mva1269I	GAATGC	1 cut(s)	337
MvaI	CCWGG	4 cut(s)	265, 900, 1122, 1468
MvnI	CGCG	2 cut(s)	26, 1242
NciI	CCSGG	1 cut(s)	138
NdeII	GATC	3 cut(s)	115, 604, 748
NlaIII	CATG	6 cut(s)	262, 711, 1012, 1046, 1399, 1416
NlaIV	GGNNCC	5 cut(s)	6, 248, 646, 670, 1799
NmuCI	GTSAC	2 cut(s)	884, 1966
NsiI	ATGCAT	1 cut(s)	1534
NspI	RCATGY	2 cut(s)	711, 1012
PaeR7I	CTCGAG	1 cut(s)	32
PaqCI	CACCTGC	1 cut(s)	1924
PasI	CCCWGGG	1 cut(s)	1121
PciI	ACATGT	1 cut(s)	1008
PciSI	GCTCTTC	2 cut(s)	68, 302
PcsI	WCGNNNNNNNCGW	1 cut(s)	30
PctI	GAATGC	1 cut(s)	337
PfeI	GAWTC	3 cut(s)	964, 1251, 1286
Pfl23II	CGTACG	1 cut(s)	1243
PflMI	CCANNNNNTGG	1 cut(s)	823
PfoI	TCCNGGA	1 cut(s)	898
PkrI	GCNGC	5 cut(s)	87, 270, 496, 1048, 1432
PleI	GAGTC	2 cut(s)	922, 1173
PmaCI	CACGTG	1 cut(s)	548
PmlI	CACGTG	1 cut(s)	548
PpsI	GAGTC	2 cut(s)	922, 1173
Ppu21I	YACGTR	1 cut(s)	548
PpuMI	RGGWCCY	1 cut(s)	247
PscI	ACATGT	1 cut(s)	1008
PsiI	TTATAA	1 cut(s)	939
Psp124BI	GAGCTC	2 cut(s)	291, 876
Psp5II	RGGWCCY	1 cut(s)	247
Psp6I	CCWGG	4 cut(s)	263, 898, 1120, 1466
PspCI	CACGTG	1 cut(s)	548
PspFI	CCCAGC	1 cut(s)	81
PspGI	CCWGG	4 cut(s)	263, 898, 1120, 1466
PspLI	CGTACG	1 cut(s)	1243
PspN4I	GGNNCC	5 cut(s)	6, 248, 646, 670, 1799
PspPI	GGNCC	8 cut(s)	79, 247, 401, 644, 668, 1502, 1719, 1798
PspPPI	RGGWCCY	1 cut(s)	247
PstNI	CAGNNNCTG	1 cut(s)	823
RsaI	GTAC	4 cut(s)	862, 1013, 1245, 1889
RsaNI	GTAC	4 cut(s)	861, 1012, 1244, 1888
RseI	CAYNNNNRTG	3 cut(s)	706, 1206, 1973
SacI	GAGCTC	2 cut(s)	291, 876
SapI	GCTCTTC	2 cut(s)	68, 302
SaqAI	TTAA	3 cut(s)	57, 1235, 1448
SatI	GCNGC	5 cut(s)	86, 269, 495, 1047, 1431
Sau3AI	GATC	3 cut(s)	115, 604, 748
Sau96I	GGNCC	8 cut(s)	79, 247, 401, 644, 668, 1502, 1719, 1798
SchI	GAGTC	2 cut(s)	922, 1173
ScrFI	CCNGG	5 cut(s)	138, 265, 900, 1122, 1468
SduI	GDGCHC	5 cut(s)	9, 291, 410, 876, 1225
SfaNI	GCATC	6 cut(s)	374, 427, 497, 1210, 1255, 1519
SfcI	CTRYAG	1 cut(s)	1562
SfiI	GGCCNNNNNGGCC	1 cut(s)	77
Sfr274I	CTCGAG	1 cut(s)	32
SinI	GGWCC	5 cut(s)	247, 401, 668, 1502, 1798
SlaI	CTCGAG	1 cut(s)	32
SmiMI	CAYNNNNRTG	3 cut(s)	706, 1206, 1973
SmlI	CTYRAG	4 cut(s)	32, 290, 368, 560
SmoI	CTYRAG	4 cut(s)	32, 290, 368, 560
Sse9I	AATT	6 cut(s)	302, 581, 1051, 1094, 1114, 1746
SsiI	CCGC	3 cut(s)	97, 269, 494
SstI	GAGCTC	2 cut(s)	291, 876
StyD4I	CCNGG	5 cut(s)	136, 263, 898, 1120, 1466
StyI	CCWWGG	2 cut(s)	192, 648
TaaI	ACNGT	5 cut(s)	284, 631, 855, 1802, 1972
TaiI	ACGT	4 cut(s)	325, 550, 1741, 1968
TaqI	TCGA	6 cut(s)	33, 66, 183, 537, 1593, 1951
TasI	AATT	6 cut(s)	302, 581, 1051, 1094, 1114, 1746
TatI	WGTACW	2 cut(s)	1011, 1887
TauI	GCSGC	2 cut(s)	271, 497
TfiI	GAWTC	3 cut(s)	964, 1251, 1286
Tru1I	TTAA	3 cut(s)	57, 1235, 1448
Tru9I	TTAA	3 cut(s)	57, 1235, 1448
TscAI	CASTG	5 cut(s)	138, 1222, 1230, 1525, 1556
TseFI	GTSAC	2 cut(s)	884, 1966
TseI	GCWGC	3 cut(s)	85, 1046, 1430
Tsp45I	GTSAC	2 cut(s)	884, 1966
TspDTI	ATGAA	6 cut(s)	330, 483, 946, 1127, 1317, 1494
TspGWI	ACGGA	3 cut(s)	393, 1261, 1974
TspRI	CASTG	5 cut(s)	138, 1222, 1230, 1525, 1556
Van91I	CCANNNNNTGG	1 cut(s)	823
VneI	GTGCAC	2 cut(s)	406, 1221
VpaK11BI	GGWCC	5 cut(s)	247, 401, 668, 1502, 1798
XagI	CCTNNNNNAGG	1 cut(s)	733
XapI	RAATTY	2 cut(s)	302, 1094
XceI	RCATGY	2 cut(s)	711, 1012
XhoI	CTCGAG	1 cut(s)	32
ZraI	GACGTC	1 cut(s)	1966
Zsp2I	ATGCAT	1 cut(s)	1534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.