Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001448

Physical Location & Seq

Genomic Coordinates Forward (+)

14025211 .. 14027522

Gene Structure 2312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29969

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGGTAATAGCCCAATATACTTACAATATCAGGATTTCATTGCGGGATGGGCAGGTGATGGAGGGGAAGAAGTGGATAACTTCCCCTTTGGAAATTGCTGAGGAGATTTCGAAGAGTTTGGCTTCGAAAGCTTTGATTGCTGAGGTCAGTGGGACTCTATGGGACATGGCAAGGCCTTTGGAGCGTGACTGTGAGCTTAAGATATTTACATTTGAAGACAACAAAGGCCGTGACACCTTCTGGTACTCTAGTGCTCATATTCTTGGACAGGCACTTGAGGCCAAGTATGGATGCAAGCTCTGTATTGGTCCCTGCACAACAAAAGAAGAGGGTTTCTACTATGATGCATACTATGGCAGTTTGGGCTTGAATGATGAGCACTTAAAGAAAATTGAATCAGAGGCAGAGAAGGCTGTTAAGGCCAAACAGTGCTTTGAGCGCATTGAAGTATCAATGGAACAGGCCCTCGAGATTTTCTCTGATAACAAATTCAAGGCTTCTTCAGCCTACTGGAGGGGAAATAAAGATCGCGAAAGTCTGCAGCGAGTTTATGGAATATCATATCCGGATAAAAAACTTTTGCAGGCATATATCAAGCAGCAGGAAGAAGCAAAAAAATATGACCATAGGTTGTTGGGTAAATCACAGGAGCTCATATTTTGTCACCCATATAGCCCGGGAAGTTGGTTCTTCACCCCCAACGGAGCTCGGATTTATAAGAAATTAATGCAGTTCATGGAAAATGAATATTTGGAAAGAGGATATCAGGAGGTTATAACACCAAACATATTCAAAATGGATTTGTTCGAAAAATCTAAGCATGCAGGACACTACAAACAGAATATGTTTCTTTTAGAGATTGAGAAAGAAGAATATGGTTTGAAGCCAATGAACTGTCCTGGTCACTGTGAAGAGTTTCGACATAGGCCTCGTTCTTATAGAGAACTTCCTCTTCGGATGGCTGAATTTGGGGATGATGCTCATATATTTTGTAGGGAGTCCCAACTGAAAGATGAACTCAAGGGTGTCCTCGACTTCATCAAGTATACCTATGACATTTTTGGTCTCTCTTTTGATGTGGAGCTATCAACAAGGCCAGAAAGTTACCATGGAGAACGAGCAACATGGGATAAAGCCGAGGCAATTCTTACAGAAGCACTAAATGAGTTTGGCAAGCCTTGGCAGATAAATGAAGGGGATGGGGCATTTTATGGACCAAAGATAGATTTTGGTGTGTGTAATGCTTTGAAGAAAAAATTTCAGTGTGCTACAATACAGCTTGATTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004829 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006435 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0030312 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071944 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon TGS tRNA-synt_2b tRNA_SAD

Protein Analysis

429

Amino Acids

49.93

Weight (kDa)

5.92

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGS	PF02824	10 - 70	3.9e-15	TGS domain
tRNA-synt_2b	PF00587	284 - 324	7.2e-07	tRNA synthetase class II core domain (G, H, P, S and T)
tRNA-synt_2b	PF00587	325 - 428	5.5e-08	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001448)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18130 AT5G26830
fragaria_vesca	FvH4_3g11970 FvH4_4g07680 FvH4_4g07680 FvH4_4g07680 FvH4_4g07690 FvH4_4g07690 FvH4_6g47820
malus_domestica	MD13G1089000.v1.1 MD16G1089100.v1.1
prunus_persica	Prupe.1G256800_v2.0.a1 Prupe.7G261600_v2.0.a1 Prupe.8G070800_v2.0.a1 Prupe.8G074400_v2.0.a1 Prupe.8G074400_v2.0.a1
pyrus_communis	pycom13g07710 pycom16g07630
rosa_chinensis	RchiOBHm_Chr4g0400731 RchiOBHm_Chr4g0444241 RchiOBHm_Chr5g0019561 RchiOBHm_Chr5g0019571 RchiOBHm_Chr5g0025481
rosa_laevigata	RLG00000005338 RLG00000008608 RLG00000009182 RLG00000032456
rosa_multiflora	Rmu_sc0002636.1_g000026 Rmu_sc0002759.1_g000056
rosa_roxburghii	Rroxscaffold_155G00443880 Rroxscaffold_1G00058510 Rroxscaffold_3G00273610 Rroxscaffold_5G00345580
rosa_rugosa	Rorug04G0025500 Rorug04G0093800 Rorug05G0052000 Rorug06G0426500
rosa_samantha	Rh4AG103300 Rh4AG411800 Rh4CG111600 Rh4DG095200 Rh5AG142600 Rh5BG141100 Rh5CG153100 Rh5CG153200 Rh5DG141700 Rh5DG141800 Rh7CG027000 Rh7CG027200 Rh7DG026700
rosa_wichuraiana	Rw4G008360 Rw5G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	717, 776
AarI	CACCTGC	1 cut(s)	43
Acc36I	ACCTGC	1 cut(s)	43
AccI	GTMKAC	1 cut(s)	1046
AccII	CGCG	1 cut(s)	531
AccIII	TCCGGA	1 cut(s)	565
AciI	CCGC	1 cut(s)	43
AcsI	RAATTY	3 cut(s)	488, 965, 1256
AcuI	CTGAAG	1 cut(s)	486
AfaI	GTAC	1 cut(s)	245
AfiI	CCNNNNNNNGG	1 cut(s)	513
AflII	CTTAAG	1 cut(s)	197
AgsI	TTSAA	8 cut(s)	215, 370, 395, 446, 493, 793, 883, 1249
AjnI	CCWGG	1 cut(s)	898
AluBI	AGCT	7 cut(s)	131, 196, 298, 652, 707, 1084, 1279
AluI	AGCT	7 cut(s)	131, 196, 298, 652, 707, 1084, 1279
Alw21I	GWGCWC	4 cut(s)	256, 381, 654, 709
Alw26I	GTCTC	1 cut(s)	1070
Ama87I	CYCGRG	2 cut(s)	467, 676
Aor13HI	TCCGGA	1 cut(s)	565
AoxI	GGCC	7 cut(s)	173, 226, 279, 420, 462, 926, 1094
ApeKI	GCWGC	2 cut(s)	541, 598
ApoI	RAATTY	3 cut(s)	488, 965, 1256
AseI	ATTAAT	1 cut(s)	725
Asp700I	GAANNNNTTC	1 cut(s)	915
AspLEI	GCGC	1 cut(s)	441
AspS9I	GGNCC	3 cut(s)	308, 463, 1214
AsuC2I	CCSGG	2 cut(s)	677, 678
AsuHPI	GGTGA	3 cut(s)	67, 656, 685
AsuII	TTCGAA	3 cut(s)	110, 125, 807
AvaI	CYCGRG	2 cut(s)	467, 676
AvaII	GGWCC	2 cut(s)	308, 1214
BaeI	ACNNNNGTAYC	2 cut(s)	235, 268
BanII	GRGCYC	2 cut(s)	654, 709
BbsI	GAAGAC	1 cut(s)	222
Bbv12I	GWGCWC	4 cut(s)	256, 381, 654, 709
BbvCI	CCTCAGC	2 cut(s)	99, 141
BbvI	GCAGC	2 cut(s)	553, 610
BccI	CCATC	4 cut(s)	41, 52, 952, 1193
BceAI	ACGGC	1 cut(s)	213
BciT130I	CCWGG	1 cut(s)	900
BcnI	CCSGG	2 cut(s)	677, 678
BcoDI	GTCTC	1 cut(s)	1070
BfaI	CTAG	1 cut(s)	249
BfmI	CTRYAG	1 cut(s)	539
BfrI	CTTAAG	1 cut(s)	197
BfuAI	ACCTGC	1 cut(s)	43
BisI	GCNGC	2 cut(s)	542, 599
BlsI	GCNGC	2 cut(s)	543, 600
Bme1390I	CCNGG	3 cut(s)	677, 678, 900
Bme18I	GGWCC	2 cut(s)	308, 1214
BmeT110I	CYCGRG	2 cut(s)	467, 676
BmgT120I	GGNCC	3 cut(s)	308, 463, 1214
BmiI	GGNNCC	1 cut(s)	310
BmrFI	CCNGG	3 cut(s)	677, 678, 900
BmsI	GCATC	3 cut(s)	281, 334, 967
BpiI	GAAGAC	1 cut(s)	222
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	1 cut(s)	532
Bpu10I	CCTNAGC	2 cut(s)	99, 141
Bpu14I	TTCGAA	3 cut(s)	110, 125, 807
BpuEI	CTTGAG	2 cut(s)	296, 1004
BpuMI	CCSGG	2 cut(s)	677, 678
BsaI	GGTCTC	1 cut(s)	1070
BsaJI	CCNNGG	4 cut(s)	676, 1108, 1137, 1178
BsaWI	WCCGGW	1 cut(s)	565
Bsc4I	CCNNNNNNNGG	1 cut(s)	513
Bse1I	ACTGG	1 cut(s)	515
Bse3DI	GCAATG	1 cut(s)	38
BseAI	TCCGGA	1 cut(s)	565
BseBI	CCWGG	1 cut(s)	900
BseDI	CCNNGG	4 cut(s)	676, 1108, 1137, 1178
BseGI	GGATG	5 cut(s)	52, 296, 963, 979, 1204
BseLI	CCNNNNNNNGG	1 cut(s)	513
BseMI	GCAATG	1 cut(s)	38
BseMII	CTCAG	2 cut(s)	90, 132
BseNI	ACTGG	1 cut(s)	515
BseRI	GAGGAG	1 cut(s)	116
BseXI	GCAGC	2 cut(s)	553, 610
BsgI	GTGCAG	1 cut(s)	298
Bsh1236I	CGCG	1 cut(s)	531
BshFI	GGCC	7 cut(s)	175, 228, 281, 422, 464, 928, 1096
BsiHKAI	GWGCWC	4 cut(s)	256, 381, 654, 709
BsiHKCI	CYCGRG	2 cut(s)	467, 676
BsiSI	CCGG	2 cut(s)	566, 677
BslFI	GGGAC	4 cut(s)	166, 176, 294, 985
BslI	CCNNNNNNNGG	1 cut(s)	513
BsmAI	GTCTC	1 cut(s)	1070
BsmFI	GGGAC	4 cut(s)	166, 176, 294, 985
BsnI	GGCC	7 cut(s)	175, 228, 281, 422, 464, 928, 1096
Bso31I	GGTCTC	1 cut(s)	1070
BsoBI	CYCGRG	2 cut(s)	467, 676
Bsp119I	TTCGAA	3 cut(s)	110, 125, 807
Bsp1286I	GDGCHC	4 cut(s)	256, 381, 654, 709
Bsp13I	TCCGGA	1 cut(s)	565
Bsp143I	GATC	1 cut(s)	526
Bsp19I	CCATGG	1 cut(s)	1108
Bsp68I	TCGCGA	1 cut(s)	531
BspACI	CCGC	1 cut(s)	43
BspANI	GGCC	7 cut(s)	175, 228, 281, 422, 464, 928, 1096
BspCNI	CTCAG	2 cut(s)	91, 133
BspEI	TCCGGA	1 cut(s)	565
BspFNI	CGCG	1 cut(s)	531
BspLI	GGNNCC	1 cut(s)	310
BspMAI	CTGCAG	1 cut(s)	543
BspMI	ACCTGC	1 cut(s)	43
BspT104I	TTCGAA	3 cut(s)	110, 125, 807
BspTI	CTTAAG	1 cut(s)	197
BspTNI	GGTCTC	1 cut(s)	1070
BsrDI	GCAATG	1 cut(s)	38
BsrI	ACTGG	1 cut(s)	515
BssECI	CCNNGG	4 cut(s)	676, 1108, 1137, 1178
BssMI	GATC	1 cut(s)	526
BssNAI	GTATAC	1 cut(s)	1047
BssT1I	CCWWGG	2 cut(s)	1108, 1178
Bst1107I	GTATAC	1 cut(s)	1047
Bst2UI	CCWGG	1 cut(s)	900
Bst4CI	ACNGT	4 cut(s)	191, 429, 896, 908
Bst6I	CTCTTC	4 cut(s)	107, 321, 906, 957
BstAFI	CTTAAG	1 cut(s)	197
BstBI	TTCGAA	3 cut(s)	110, 125, 807
BstC8I	GCNNGC	4 cut(s)	296, 585, 822, 1175
BstDEI	CTNAG	3 cut(s)	99, 141, 816
BstDSI	CCRYGG	1 cut(s)	1108
BstENI	CCTNNNNNAGG	1 cut(s)	511
BstF5I	GGATG	5 cut(s)	52, 296, 963, 979, 1204
BstFNI	CGCG	1 cut(s)	531
BstHHI	GCGC	1 cut(s)	441
BstKTI	GATC	1 cut(s)	529
BstMAI	GTCTC	1 cut(s)	1070
BstMBI	GATC	1 cut(s)	526
BstNI	CCWGG	1 cut(s)	900
BstNSI	RCATGY	1 cut(s)	824
BstSCI	CCNGG	3 cut(s)	675, 676, 898
BstSFI	CTRYAG	1 cut(s)	539
BstUI	CGCG	1 cut(s)	531
BstV1I	GCAGC	2 cut(s)	553, 610
BstV2I	GAAGAC	1 cut(s)	222
BstZ17I	GTATAC	1 cut(s)	1047
BsuRI	GGCC	7 cut(s)	175, 228, 281, 422, 464, 928, 1096
BtgI	CCRYGG	1 cut(s)	1108
BtsCI	GGATG	5 cut(s)	52, 296, 963, 979, 1204
BtsIMutI	CAGTG	4 cut(s)	154, 434, 904, 1268
BtuMI	TCGCGA	1 cut(s)	531
BveI	ACCTGC	1 cut(s)	43
Cac8I	GCNNGC	4 cut(s)	296, 585, 822, 1175
CfoI	GCGC	1 cut(s)	441
Cfr13I	GGNCC	3 cut(s)	308, 463, 1214
Cfr9I	CCCGGG	1 cut(s)	676
Csp6I	GTAC	1 cut(s)	244
CviAII	CATG	5 cut(s)	166, 736, 821, 1109, 1125
CviQI	GTAC	1 cut(s)	244
DdeI	CTNAG	3 cut(s)	99, 141, 816
DpnI	GATC	1 cut(s)	528
DpnII	GATC	1 cut(s)	526
Eam1104I	CTCTTC	4 cut(s)	107, 321, 906, 957
EarI	CTCTTC	4 cut(s)	107, 321, 906, 957
Ecl136II	GAGCTC	2 cut(s)	652, 707
Eco130I	CCWWGG	2 cut(s)	1108, 1178
Eco147I	AGGCCT	2 cut(s)	175, 928
Eco24I	GRGCYC	2 cut(s)	654, 709
Eco31I	GGTCTC	1 cut(s)	1070
Eco32I	GATATC	1 cut(s)	764
Eco47I	GGWCC	2 cut(s)	308, 1214
Eco53kI	GAGCTC	2 cut(s)	652, 707
Eco57I	CTGAAG	1 cut(s)	486
Eco88I	CYCGRG	2 cut(s)	467, 676
EcoICRI	GAGCTC	2 cut(s)	652, 707
EcoNI	CCTNNNNNAGG	1 cut(s)	511
EcoO109I	RGGNCCY	1 cut(s)	463
EcoRII	CCWGG	1 cut(s)	898
EcoRV	GATATC	1 cut(s)	764
EcoT14I	CCWWGG	2 cut(s)	1108, 1178
EcoT22I	ATGCAT	1 cut(s)	349
EcoT38I	GRGCYC	2 cut(s)	654, 709
ErhI	CCWWGG	2 cut(s)	1108, 1178
FaeI	CATG	5 cut(s)	169, 739, 824, 1112, 1128
FaqI	GGGAC	4 cut(s)	166, 176, 294, 985
FatI	CATG	5 cut(s)	165, 735, 820, 1108, 1124
FauI	CCCGC	1 cut(s)	36
FblI	GTMKAC	1 cut(s)	1046
Fnu4HI	GCNGC	2 cut(s)	542, 599
FokI	GGATG	5 cut(s)	59, 303, 970, 986, 1211
FriOI	GRGCYC	2 cut(s)	654, 709
Fsp4HI	GCNGC	2 cut(s)	542, 599
FspBI	CTAG	1 cut(s)	249
GlaI	GCGC	1 cut(s)	440
GluI	GCNGC	2 cut(s)	542, 599
GsuI	CTGGAG	1 cut(s)	532
HaeIII	GGCC	7 cut(s)	175, 228, 281, 422, 464, 928, 1096
HapII	CCGG	2 cut(s)	566, 677
HhaI	GCGC	1 cut(s)	441
Hin1II	CATG	5 cut(s)	169, 739, 824, 1112, 1128
Hin6I	GCGC	1 cut(s)	439
HinP1I	GCGC	1 cut(s)	439
HindIII	AAGCTT	1 cut(s)	129
HinfI	GANTC	3 cut(s)	154, 395, 998
HpaII	CCGG	2 cut(s)	566, 677
HphI	GGTGA	3 cut(s)	67, 656, 685
Hpy166II	GTNNAC	1 cut(s)	1047
Hpy188I	TCNGA	4 cut(s)	400, 481, 711, 957
Hpy188III	TCNNGA	5 cut(s)	31, 469, 530, 566, 767
Hpy8I	GTNNAC	1 cut(s)	1047
HpyAV	CCTTC	3 cut(s)	247, 403, 1187
HpyCH4III	ACNGT	4 cut(s)	191, 429, 896, 908
HpyCH4V	TGCA	7 cut(s)	294, 315, 347, 541, 583, 730, 824
HpyF3I	CTNAG	3 cut(s)	99, 141, 816
Hsp92II	CATG	5 cut(s)	169, 739, 824, 1112, 1128
HspAI	GCGC	1 cut(s)	439
Kpn2I	TCCGGA	1 cut(s)	565
Kzo9I	GATC	1 cut(s)	526
LmnI	GCTCC	4 cut(s)	181, 649, 704, 1081
Lsp1109I	GCAGC	2 cut(s)	553, 610
LweI	GCATC	3 cut(s)	281, 334, 967
MaeI	CTAG	1 cut(s)	249
MaeIII	GTNAC	5 cut(s)	185, 230, 662, 902, 1103
MalI	GATC	1 cut(s)	528
MboI	GATC	1 cut(s)	526
MhlI	GDGCHC	4 cut(s)	256, 381, 654, 709
MluCI	AATT	7 cut(s)	93, 390, 488, 722, 965, 1143, 1256
MlyI	GAGTC	2 cut(s)	148, 1007
Mph1103I	ATGCAT	1 cut(s)	349
MroI	TCCGGA	1 cut(s)	565
MroXI	GAANNNNTTC	1 cut(s)	915
MseI	TTAA	4 cut(s)	198, 383, 417, 725
MspCI	CTTAAG	1 cut(s)	197
MspI	CCGG	2 cut(s)	566, 677
MspR9I	CCNGG	3 cut(s)	677, 678, 900
MvaI	CCWGG	1 cut(s)	900
MvnI	CGCG	1 cut(s)	531
NciI	CCSGG	2 cut(s)	677, 678
NcoI	CCATGG	1 cut(s)	1108
NdeII	GATC	1 cut(s)	526
NlaIII	CATG	5 cut(s)	169, 739, 824, 1112, 1128
NlaIV	GGNNCC	1 cut(s)	310
NmeAIII	GCCGAG	1 cut(s)	1162
NmuCI	GTSAC	4 cut(s)	185, 230, 662, 902
NruI	TCGCGA	1 cut(s)	531
NsiI	ATGCAT	1 cut(s)	349
NspI	RCATGY	1 cut(s)	824
NspV	TTCGAA	3 cut(s)	110, 125, 807
PaeI	GCATGC	1 cut(s)	824
PaeR7I	CTCGAG	1 cut(s)	467
PaqCI	CACCTGC	1 cut(s)	43
PceI	AGGCCT	2 cut(s)	175, 928
PdmI	GAANNNNTTC	1 cut(s)	915
PfeI	GAWTC	1 cut(s)	395
PkrI	GCNGC	2 cut(s)	543, 600
PleI	GAGTC	2 cut(s)	148, 1006
PpsI	GAGTC	2 cut(s)	148, 1006
PshBI	ATTAAT	1 cut(s)	725
PsiI	TTATAA	2 cut(s)	717, 776
Psp124BI	GAGCTC	2 cut(s)	654, 709
Psp6I	CCWGG	1 cut(s)	898
PspGI	CCWGG	1 cut(s)	898
PspN4I	GGNNCC	1 cut(s)	310
PspPI	GGNCC	3 cut(s)	308, 463, 1214
PstI	CTGCAG	1 cut(s)	543
RruI	TCGCGA	1 cut(s)	531
RsaI	GTAC	1 cut(s)	245
RsaNI	GTAC	1 cut(s)	244
SacI	GAGCTC	2 cut(s)	654, 709
SaqAI	TTAA	4 cut(s)	198, 383, 417, 725
SatI	GCNGC	2 cut(s)	542, 599
Sau3AI	GATC	1 cut(s)	526
Sau96I	GGNCC	3 cut(s)	308, 463, 1214
SchI	GAGTC	2 cut(s)	148, 1007
ScrFI	CCNGG	3 cut(s)	677, 678, 900
SduI	GDGCHC	4 cut(s)	256, 381, 654, 709
SfaNI	GCATC	3 cut(s)	281, 334, 967
SfcI	CTRYAG	1 cut(s)	539
Sfr274I	CTCGAG	1 cut(s)	467
SfuI	TTCGAA	3 cut(s)	110, 125, 807
SinI	GGWCC	2 cut(s)	308, 1214
SlaI	CTCGAG	1 cut(s)	467
SmaI	CCCGGG	1 cut(s)	678
SmlI	CTYRAG	4 cut(s)	197, 275, 467, 1019
SmoI	CTYRAG	4 cut(s)	197, 275, 467, 1019
SphI	GCATGC	1 cut(s)	824
Sse9I	AATT	7 cut(s)	93, 390, 488, 722, 965, 1143, 1256
SseBI	AGGCCT	2 cut(s)	175, 928
SsiI	CCGC	1 cut(s)	43
SspI	AATATT	1 cut(s)	749
SspMI	CTAG	1 cut(s)	249
SstI	GAGCTC	2 cut(s)	654, 709
StuI	AGGCCT	2 cut(s)	175, 928
StyD4I	CCNGG	3 cut(s)	675, 676, 898
StyI	CCWWGG	2 cut(s)	1108, 1178
TaaI	ACNGT	4 cut(s)	191, 429, 896, 908
TaqI	TCGA	6 cut(s)	110, 125, 468, 807, 919, 1032
TasI	AATT	7 cut(s)	93, 390, 488, 722, 965, 1143, 1256
TfiI	GAWTC	1 cut(s)	395
Tru1I	TTAA	4 cut(s)	198, 383, 417, 725
Tru9I	TTAA	4 cut(s)	198, 383, 417, 725
TscAI	CASTG	4 cut(s)	154, 434, 911, 1268
TseFI	GTSAC	4 cut(s)	185, 230, 662, 902
TseI	GCWGC	2 cut(s)	541, 598
Tsp45I	GTSAC	4 cut(s)	185, 230, 662, 902
TspDTI	ATGAA	7 cut(s)	27, 724, 759, 905, 1027, 1029, 1206
TspGWI	ACGGA	1 cut(s)	717
TspMI	CCCGGG	1 cut(s)	676
TspRI	CASTG	4 cut(s)	154, 434, 911, 1268
Vha464I	CTTAAG	1 cut(s)	197
VpaK11BI	GGWCC	2 cut(s)	308, 1214
VspI	ATTAAT	1 cut(s)	725
XagI	CCTNNNNNAGG	1 cut(s)	511
XapI	RAATTY	3 cut(s)	488, 965, 1256
XceI	RCATGY	1 cut(s)	824
XhoI	CTCGAG	1 cut(s)	467
XmaI	CCCGGG	1 cut(s)	676
XmiI	GTMKAC	1 cut(s)	1046
XmnI	GAANNNNTTC	1 cut(s)	915
XspI	CTAG	1 cut(s)	249
Zsp2I	ATGCAT	1 cut(s)	349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.