AT5G50375

cycloeucalenol cycloisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008396
Physical Location & Seq
Forward (+)
20511642 .. 20514346
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50375.2

Sequence Viewer

Length: 843 bp
ATGTCAGGATCTTCTTCACCGAGCTTGTGGCTGGCACCGAATCCGAGCAAGAGATGGGGAGAGCTCTTCTTCCTCTTCTACACTCCTTTTTGGCTCACTCTCTGTTTAGGCATCGTTGTTCCTTACAAGCTTTACGAGACATTCACGGAGTTGGAGTATCTGCTTCTAGCTTTGGTTTCAGCTGTTCCTGCTTTCGTTATACCCATGTTACTCGTTGGAAAGGCCGACAGAAGTTTATGTTGGAAGGACCGCTATTGGGTTAAGGCAAATCTCTGGATAATTGTTTTCAGCTATGTCGGAAACTACTTTTGGACTCACTATTTCTTTAAAGTTCTTGGAGCGTCCTATACTTTTCCATCATGGAAAATGAATAATGTGCCTCACACAACATTCTTCCTAACACATGTTTGCTTCCTCTTTTACCACGTTGCATCGAACATTACTCTTCGGAGGCTACGCCATTCCACTGCTGATTTACCAGATTCTCTGAAATGGTGTTTTGAGGCTGCATGGATACTTGCGCTTTCTTATTTCATTGCATACTTGGAGACTATTGCTATCGCAAATTTTCCTTACTATGAGTTTGTGGACCGAAGTGCCATGTACAGAGTTGGATGTCTTTTCTATGCCATTTACTTCATTGTGAGCTTCCCAATGTTCTTCAGGATGGATGAGAAATCAACTGATGAATGGGATTTATCTCGAGTGGCTGTTGATGCTTTGGGTGCTGCTATGTTGGTAACAATCATTCTTGATCTATGGCGTCTCTTCTTGGGACCTATAGTTCCCTTACCGGAGGGACAAAACTGCCTTCAGTCTGGATTACCATGGTTCTCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

32.64

Weight (kDa)

6.94

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 34
AciI CCGC 1 cut(s) 250
AclWI GGATC 1 cut(s) 16
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 2 cut(s) 646, 797
AcyI GRCGYC 1 cut(s) 763
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 403
AjuI GAANNNNNNNTTGG 4 cut(s) 223, 255, 292, 324
AloI GAACNNNNNNTCC 2 cut(s) 768, 800
AluBI AGCT 7 cut(s) 24, 64, 130, 170, 182, 291, 648
AluI AGCT 7 cut(s) 24, 64, 130, 170, 182, 291, 648
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 3 cut(s) 131, 542, 770
AlwI GGATC 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 506, 728
ApoI RAATTY 1 cut(s) 565
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 3 cut(s) 247, 589, 776
AsuHPI GGTGA 1 cut(s) 9
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 3 cut(s) 247, 589, 776
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 493, 715
BccI CCATC 3 cut(s) 48, 364, 661
BciVI GTATCC 1 cut(s) 507
BcoDI GTCTC 3 cut(s) 131, 542, 770
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 780
BfuI GTATCC 1 cut(s) 507
BisI GCNGC 2 cut(s) 507, 729
BlsI GCNGC 2 cut(s) 508, 730
Bme18I GGWCC 3 cut(s) 247, 589, 776
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 3 cut(s) 247, 589, 776
BmiI GGNNCC 2 cut(s) 36, 777
BmsI GCATC 3 cut(s) 120, 440, 706
BsaHI GRCGYC 1 cut(s) 763
BsaJI CCNNGG 1 cut(s) 827
BsaWI WCCGGW 1 cut(s) 793
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 827
BseGI GGATG 3 cut(s) 620, 672, 676
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 534
BseXI GCAGC 2 cut(s) 493, 715
BshFI GGCC 1 cut(s) 224
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 2 cut(s) 789, 813
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 3 cut(s) 131, 542, 770
BsmBI CGTCTC 1 cut(s) 770
BsmFI GGGAC 2 cut(s) 789, 813
BsnI GGCC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 2 cut(s) 8, 754
Bsp19I CCATGG 1 cut(s) 827
BspACI CCGC 1 cut(s) 250
BspANI GGCC 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 36, 777
BspPI GGATC 1 cut(s) 16
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 827
BssMI GATC 2 cut(s) 8, 754
BssNI GRCGYC 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 827
Bst6I CTCTTC 4 cut(s) 71, 80, 450, 773
BstACI GRCGYC 1 cut(s) 763
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 827
BstF5I GGATG 3 cut(s) 620, 672, 676
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 2 cut(s) 11, 757
BstMAI GTCTC 3 cut(s) 131, 542, 770
BstMBI GATC 2 cut(s) 8, 754
BstMWI GCNNNNNNNGC 3 cut(s) 188, 716, 725
BstNSI RCATGY 1 cut(s) 407
BstSFI CTRYAG 1 cut(s) 780
BstV1I GCAGC 2 cut(s) 493, 715
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuI GTATCC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 827
BtsCI GGATG 3 cut(s) 620, 672, 676
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 33
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 247, 589, 776
CseI GACGC 2 cut(s) 330, 752
Csp6I GTAC 1 cut(s) 604
CviAII CATG 6 cut(s) 205, 360, 404, 510, 601, 828
CviQI GTAC 1 cut(s) 604
DpnI GATC 2 cut(s) 10, 756
DpnII GATC 2 cut(s) 8, 754
DraI TTTAAA 1 cut(s) 328
Eam1104I CTCTTC 4 cut(s) 71, 80, 450, 773
EarI CTCTTC 4 cut(s) 71, 80, 450, 773
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 827
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 3 cut(s) 247, 589, 776
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 646, 797
Eco88I CYCGRG 1 cut(s) 702
EcoICRI GAGCTC 1 cut(s) 64
EcoO109I RGGNCCY 1 cut(s) 776
EcoT14I CCWWGG 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 1 cut(s) 770
FaeI CATG 6 cut(s) 208, 363, 407, 513, 604, 831
FaqI GGGAC 2 cut(s) 789, 813
FatI CATG 6 cut(s) 204, 359, 403, 509, 600, 827
Fnu4HI GCNGC 2 cut(s) 507, 729
FokI GGATG 3 cut(s) 627, 679, 683
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 2 cut(s) 507, 729
FspBI CTAG 1 cut(s) 167
GlaI GCGC 1 cut(s) 522
GluI GCNGC 2 cut(s) 507, 729
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 794
HgaI GACGC 2 cut(s) 330, 752
HhaI GCGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 763
Hin1II CATG 6 cut(s) 208, 363, 407, 513, 604, 831
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 40, 313, 482
HpaII CCGG 1 cut(s) 794
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 4 cut(s) 45, 299, 450, 489
Hpy188III TCNNGA 6 cut(s) 6, 274, 664, 702, 752, 819
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 2 cut(s) 238, 821
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 3 cut(s) 431, 509, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 716, 725
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 763
Hsp92II CATG 6 cut(s) 208, 363, 407, 513, 604, 831
HspAI GCGC 1 cut(s) 521
Kzo9I GATC 2 cut(s) 8, 754
LguI GCTCTTC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 338
LpnPI CCDG 7 cut(s) 17, 201, 259, 492, 649, 804, 807
Lsp1109I GCAGC 2 cut(s) 493, 715
LweI GCATC 3 cut(s) 120, 440, 706
MaeI CTAG 1 cut(s) 167
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 207, 739
MalI GATC 2 cut(s) 10, 756
MboI GATC 2 cut(s) 8, 754
MboII GAAGA 9 cut(s) 3, 6, 58, 61, 67, 385, 437, 652, 760
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 3 cut(s) 279, 565, 838
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 5 cut(s) 132, 196, 221, 277, 592
MnlI CCTC 6 cut(s) 83, 390, 425, 444, 496, 790
MseI TTAA 2 cut(s) 261, 327
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 1 cut(s) 794
MunI CAATTG 1 cut(s) 838
MwoI GCNNNNNNNGC 3 cut(s) 188, 716, 725
NcoI CCATGG 1 cut(s) 827
NdeII GATC 2 cut(s) 8, 754
NlaIII CATG 6 cut(s) 208, 363, 407, 513, 604, 831
NlaIV GGNNCC 2 cut(s) 36, 777
NspI RCATGY 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 702
PciI ACATGT 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 40, 482
PkrI GCNGC 2 cut(s) 508, 730
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 776
PscI ACATGT 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 776
PspN4I GGNNCC 2 cut(s) 36, 777
PspPI GGNCC 3 cut(s) 247, 589, 776
PspPPI RGGWCCY 1 cut(s) 776
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 261, 327
SatI GCNGC 2 cut(s) 507, 729
Sau3AI GATC 2 cut(s) 8, 754
Sau96I GGNCC 3 cut(s) 247, 589, 776
SchI GAGTC 1 cut(s) 307
SduI GDGCHC 1 cut(s) 66
SetI ASST 9 cut(s) 26, 66, 132, 172, 184, 293, 429, 650, 781
SfaNI GCATC 3 cut(s) 120, 440, 706
SfcI CTRYAG 1 cut(s) 780
Sfr274I CTCGAG 1 cut(s) 702
SinI GGWCC 3 cut(s) 247, 589, 776
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 702
SmoI CTYRAG 1 cut(s) 702
Sse9I AATT 3 cut(s) 279, 565, 838
SsiI CCGC 1 cut(s) 250
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 1 cut(s) 827
TaiI ACGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 434, 703
TaqII GACCGA 1 cut(s) 606
TasI AATT 3 cut(s) 279, 565, 838
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 2 cut(s) 40, 482
Tru1I TTAA 2 cut(s) 261, 327
Tru9I TTAA 2 cut(s) 261, 327
TscAI CASTG 1 cut(s) 472
TseI GCWGC 2 cut(s) 506, 728
TspDTI ATGAA 4 cut(s) 383, 523, 628, 702
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 1 cut(s) 472
VpaK11BI GGWCC 3 cut(s) 247, 589, 776
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 702
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.