Rw7G009610

Cycloeucalenol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008396
Physical Location & Seq
Reverse (-)
8960033 .. 8963307
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009610.1

Sequence Viewer

Length: 873 bp
ATGGGTGGTAAAAAGGAATCTTCTTCTTCTTCTCCGAGTTTATGGCTGGCTTCCAATCCAAGCAAGCGATGGGGGGAGCTCTTCTTTCTCTTCTACACTCCTTTCTGGCTCACTCTCTGCCTCGGAATCGTCGTCCCCTACAAGCTCTACGAGAGGTTTACGGAATGGGAGTATTTGCTAATTGGATTGGTCTCGGCTGTTCCTTCGTTTTTGTTACCCATGTTATTCGTTGGAAAGGCAGATAGAAGCTTGTGTTGGAAGGATCGGTATTGGGTCAAGGCCAGTGTGTGGACAATACTTTTTAGCTATGTTGGGAATTACTTTTGGACCCACTATTTCTTCACGGTTTTGGGAGCTTCGTATACTTTCCCGTCATGGAAAATGAACAATGTACCACATACTACCTTCCTTCTTACACATGTTTGCTTCCTATTTTATCATGTCGGATCAAACATGACACTTCGCAGACTACGCCATTCTATTGCTGACATGCCAAAACAAGTTCAATTGCTTTTTGAGGCTGCATGGATATTGGCTTTTGCTTATTTCATAGCATACCTGGAGACTGTAGCTATTTCCAACTTCCCTTATTATGAATTTGTCGACCGGGCGATTATGTACAAAGTTGGGTGCTTGTTTTATGCCATCTACTTCATTGTTAGCTTTCCCATGTTTCTTAGGATTGACGAGAAACCTGGTGATGCATGGGACTTGCCAAGAGCGGCCATTGATGCTTTGGGTGCTGCAATGCTGGTGACGATAATACTAGATCTATGGCGTATATTTCTGGGACCTATTGTTTCGATTCCAGAAACTAAACAGTGCATTCAACCCGGACTACCATGGTTCCCTGGACATGACAACATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

33.73

Weight (kDa)

8.49

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 288
AccBSI CCGCTC 1 cut(s) 722
AccI GTMKAC 2 cut(s) 362, 603
AciI CCGC 1 cut(s) 722
AclWI GGATC 2 cut(s) 270, 454
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 1 cut(s) 596
AfaI GTAC 2 cut(s) 393, 620
AfiI CCNNNNNNNGG 1 cut(s) 288
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 2 cut(s) 506, 830
AjnI CCWGG 3 cut(s) 558, 694, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 238, 270
AluBI AGCT 7 cut(s) 79, 145, 249, 306, 356, 572, 663
AluI AGCT 7 cut(s) 79, 145, 249, 306, 356, 572, 663
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 196, 557
AlwI GGATC 2 cut(s) 270, 454
AoxI GGCC 2 cut(s) 279, 723
ApeKI GCWGC 2 cut(s) 521, 743
ApoI RAATTY 1 cut(s) 596
AspS9I GGNCC 2 cut(s) 327, 791
AsuC2I CCSGG 2 cut(s) 608, 834
AsuHPI GGTGA 2 cut(s) 710, 766
AvaII GGWCC 2 cut(s) 327, 791
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 508, 730
BccI CCATC 2 cut(s) 63, 653
BciT130I CCWGG 3 cut(s) 560, 696, 852
BcnI CCSGG 2 cut(s) 608, 834
BcoDI GTCTC 2 cut(s) 196, 557
BfaI CTAG 1 cut(s) 767
BfmI CTRYAG 1 cut(s) 567
BglII AGATCT 1 cut(s) 769
BisI GCNGC 3 cut(s) 522, 723, 744
BlsI GCNGC 3 cut(s) 523, 724, 745
Bme1390I CCNGG 5 cut(s) 560, 608, 696, 834, 852
Bme18I GGWCC 2 cut(s) 327, 791
BmgT120I GGNCC 2 cut(s) 327, 791
BmiI GGNNCC 3 cut(s) 329, 792, 848
BmrFI CCNGG 5 cut(s) 560, 608, 696, 834, 852
BmsI GCATC 2 cut(s) 691, 721
BpmI CTGGAG 1 cut(s) 581
BpuMI CCSGG 2 cut(s) 608, 834
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 3 cut(s) 121, 842, 850
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 3 cut(s) 560, 696, 852
BseDI CCNNGG 3 cut(s) 121, 842, 850
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMI GCAATG 1 cut(s) 753
BseNI ACTGG 1 cut(s) 282
BseXI GCAGC 2 cut(s) 508, 730
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 2 cut(s) 281, 725
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 2 cut(s) 607, 834
BslFI GGGAC 3 cut(s) 119, 722, 804
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 2 cut(s) 196, 557
BsmFI GGGAC 3 cut(s) 119, 722, 804
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 2 cut(s) 281, 725
Bso31I GGTCTC 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 81
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 3 cut(s) 262, 446, 769
Bsp19I CCATGG 1 cut(s) 842
BspACI CCGC 1 cut(s) 722
BspANI GGCC 2 cut(s) 281, 725
BspLI GGNNCC 3 cut(s) 329, 792, 848
BspPI GGATC 2 cut(s) 270, 454
BspQI GCTCTTC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 196
BsrBI CCGCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 753
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 3 cut(s) 121, 842, 850
BssMI GATC 3 cut(s) 262, 446, 769
BssNAI GTATAC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 842
Bst1107I GTATAC 1 cut(s) 363
Bst2UI CCWGG 3 cut(s) 560, 696, 852
Bst4CI ACNGT 3 cut(s) 346, 568, 822
Bst6I CTCTTC 2 cut(s) 86, 95
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 48, 65
BstDEI CTNAG 1 cut(s) 677
BstDSI CCRYGG 1 cut(s) 842
BstKTI GATC 3 cut(s) 265, 449, 772
BstMAI GTCTC 2 cut(s) 196, 557
BstMBI GATC 3 cut(s) 262, 446, 769
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 3 cut(s) 471, 731, 740
BstNI CCWGG 3 cut(s) 560, 696, 852
BstNSI RCATGY 2 cut(s) 422, 493
BstSCI CCNGG 5 cut(s) 558, 606, 694, 832, 850
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 508, 730
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZ17I GTATAC 1 cut(s) 363
BsuRI GGCC 2 cut(s) 281, 725
BtgI CCRYGG 1 cut(s) 842
BtgZI GCGATG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 289, 827
Cac8I GCNNGC 2 cut(s) 48, 65
Cfr13I GGNCC 2 cut(s) 327, 791
CsiI ACCWGGT 1 cut(s) 694
Csp6I GTAC 2 cut(s) 392, 619
CviQI GTAC 2 cut(s) 392, 619
DdeI CTNAG 1 cut(s) 677
DpnI GATC 3 cut(s) 264, 448, 771
DpnII GATC 3 cut(s) 262, 446, 769
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 2 cut(s) 86, 95
EarI CTCTTC 2 cut(s) 86, 95
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 842
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 327, 791
Eco53kI GAGCTC 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 791
EcoRII CCWGG 3 cut(s) 558, 694, 850
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 842
FaqI GGGAC 3 cut(s) 119, 722, 804
FblI GTMKAC 2 cut(s) 362, 603
Fnu4HI GCNGC 3 cut(s) 522, 723, 744
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 522, 723, 744
FspBI CTAG 1 cut(s) 767
GluI GCNGC 3 cut(s) 522, 723, 744
GsuI CTGGAG 1 cut(s) 581
HaeIII GGCC 2 cut(s) 281, 725
HapII CCGG 2 cut(s) 607, 834
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 3 cut(s) 17, 126, 805
HpaII CCGG 2 cut(s) 607, 834
HphI GGTGA 2 cut(s) 710, 766
Hpy166II GTNNAC 4 cut(s) 159, 291, 363, 604
Hpy188I TCNGA 3 cut(s) 36, 125, 446
Hpy188III TCNNGA 1 cut(s) 809
Hpy8I GTNNAC 4 cut(s) 159, 291, 363, 604
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 4 cut(s) 213, 253, 415, 419
HpyCH4III ACNGT 3 cut(s) 346, 568, 822
HpyCH4V TGCA 4 cut(s) 524, 704, 746, 825
HpyF10VI GCNNNNNNNGC 3 cut(s) 471, 731, 740
HpyF3I CTNAG 1 cut(s) 677
Kzo9I GATC 3 cut(s) 262, 446, 769
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 2 cut(s) 76, 353
Lsp1109I GCAGC 2 cut(s) 508, 730
LweI GCATC 2 cut(s) 691, 721
MabI ACCWGGT 1 cut(s) 694
MaeI CTAG 1 cut(s) 767
MaeIII GTNAC 2 cut(s) 213, 754
MalI GATC 3 cut(s) 264, 448, 771
MbiI CCGCTC 1 cut(s) 722
MboI GATC 3 cut(s) 262, 446, 769
MboII GAAGA 7 cut(s) 12, 15, 18, 21, 73, 82, 331
MfeI CAATTG 1 cut(s) 506
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 4 cut(s) 180, 316, 506, 596
MmeI TCCRAC 4 cut(s) 211, 236, 424, 603
MnlI CCTC 3 cut(s) 131, 147, 511
Mph1103I ATGCAT 1 cut(s) 706
MspI CCGG 2 cut(s) 607, 834
MspR9I CCNGG 5 cut(s) 560, 608, 696, 834, 852
MunI CAATTG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 3 cut(s) 560, 696, 852
MwoI GCNNNNNNNGC 3 cut(s) 471, 731, 740
NciI CCSGG 2 cut(s) 608, 834
NcoI CCATGG 1 cut(s) 842
NdeII GATC 3 cut(s) 262, 446, 769
NlaIV GGNNCC 3 cut(s) 329, 792, 848
NmeAIII GCCGAG 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 2 cut(s) 422, 493
PciI ACATGT 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 825
PfeI GAWTC 3 cut(s) 17, 126, 805
PflMI CCANNNNNTGG 1 cut(s) 288
PkrI GCNGC 3 cut(s) 523, 724, 745
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 3 cut(s) 558, 694, 850
PspGI CCWGG 3 cut(s) 558, 694, 850
PspN4I GGNNCC 3 cut(s) 329, 792, 848
PspPI GGNCC 2 cut(s) 327, 791
PspPPI RGGWCCY 1 cut(s) 791
PsuI RGATCY 1 cut(s) 769
RsaI GTAC 2 cut(s) 393, 620
RsaNI GTAC 2 cut(s) 392, 619
SacI GAGCTC 1 cut(s) 81
SalI GTCGAC 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 86
SatI GCNGC 3 cut(s) 522, 723, 744
Sau3AI GATC 3 cut(s) 262, 446, 769
Sau96I GGNCC 2 cut(s) 327, 791
ScrFI CCNGG 5 cut(s) 560, 608, 696, 834, 852
SduI GDGCHC 1 cut(s) 81
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 2 cut(s) 691, 721
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 2 cut(s) 327, 791
Sse9I AATT 4 cut(s) 180, 316, 506, 596
SsiI CCGC 1 cut(s) 722
SspMI CTAG 1 cut(s) 767
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 5 cut(s) 558, 606, 694, 832, 850
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 3 cut(s) 346, 568, 822
TaqI TCGA 2 cut(s) 603, 803
TasI AATT 4 cut(s) 180, 316, 506, 596
TatI WGTACW 1 cut(s) 618
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 3 cut(s) 17, 126, 805
TscAI CASTG 2 cut(s) 289, 827
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 2 cut(s) 521, 743
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 4 cut(s) 398, 538, 609, 643
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 289, 827
Van91I CCANNNNNTGG 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 327, 791
XapI RAATTY 1 cut(s) 596
XceI RCATGY 2 cut(s) 422, 493
XcmI CCANNNNNNNNNTGG 2 cut(s) 66, 733
XmiI GTMKAC 2 cut(s) 362, 603
XspI CTAG 1 cut(s) 767
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.