Rroxscaffold_3G00264610

Cycloeucalenol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008396
Physical Location & Seq
Forward (+)
58200291 .. 58203621
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264610.1

Sequence Viewer

Length: 726 bp
ATGGGTGGTAAAAAGGAATCTTCTTCTTCTTCTTCTTCTTCTCCGAGTTTATGGCTGGCTTCCAATCCAAGCAAGCGCTGGGGGAGCTCTTCTTTCTCTTCTACACTCCTTTCTGGCTCACTCTCTGCCTCGGAATCGTCGTCCCCTACAAGCTCTACGAGGCAGATAGAAGCTTGTGTTGGAAGGATCGGTATTGGGTCAAGGCAAGTGCTTTCCTGGGGCGAACTTCTTTATATTGGACCTGTACCACATACTACCTTCCTTCTCACACATGTTTGCTTCCTATTTTATCATGTCGGATCAAACATGACGCTTCGCAGACTACGCCATTCTATTGCTGACATGCCAAAACAAGTTCAATTGCTTTTTGAGGCTGCATGGATATTGGCTTTTGCTTATTTCATAGCATACCTGGAGACTGTAGCTATTTCCAACTTCCCTTATTATGAATTTGTCGACCGGGCGATTATGTACAAAGTTGGGTGCTTGTTTTATGCCATCTACTTCATTGTTAGCTTTCCCATGTTTCTTAGGATTGACGAGAAACCTGGTGATGCATGGGACTTGCCAAGAGCCGCCATTGATGCTCTGGGTGCTGCAATGCTGGTGACGATAATACTAGATCTGTGGCGCATATTTCTGGGACCTATTGTTCCGATTCCAGAAACTAAACAGTGCATTCAACCCGGACTACCATGGTTCCCTGGACATGACAACTTGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.76

Weight (kDa)

7.7

Isoelectric Point (pI)

56.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 456
AciI CCGC 1 cut(s) 576
AclWI GGATC 2 cut(s) 194, 307
AcsI RAATTY 1 cut(s) 449
AfaI GTAC 2 cut(s) 246, 473
AfeI AGCGCT 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 2 cut(s) 359, 683
AjnI CCWGG 4 cut(s) 215, 411, 547, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
AluBI AGCT 5 cut(s) 87, 153, 173, 425, 516
AluI AGCT 5 cut(s) 87, 153, 173, 425, 516
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 2 cut(s) 194, 307
Aor51HI AGCGCT 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 374, 596
ApoI RAATTY 1 cut(s) 449
AspLEI GCGC 2 cut(s) 78, 633
AspS9I GGNCC 2 cut(s) 239, 644
AsuC2I CCSGG 2 cut(s) 461, 687
AsuHPI GGTGA 2 cut(s) 563, 619
AvaII GGWCC 2 cut(s) 239, 644
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 361, 583
BccI CCATC 1 cut(s) 506
BciT130I CCWGG 4 cut(s) 217, 413, 549, 705
BcnI CCSGG 2 cut(s) 461, 687
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 1 cut(s) 620
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 79
BglII AGATCT 1 cut(s) 622
BisI GCNGC 3 cut(s) 375, 576, 597
BlsI GCNGC 3 cut(s) 376, 577, 598
Bme1390I CCNGG 6 cut(s) 217, 413, 461, 549, 687, 705
Bme18I GGWCC 2 cut(s) 239, 644
BmgT120I GGNCC 2 cut(s) 239, 644
BmiI GGNNCC 2 cut(s) 645, 701
BmrFI CCNGG 6 cut(s) 217, 413, 461, 549, 687, 705
BmsI GCATC 2 cut(s) 544, 574
BpmI CTGGAG 1 cut(s) 434
BpuMI CCSGG 2 cut(s) 461, 687
BsaJI CCNNGG 4 cut(s) 129, 216, 695, 703
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 4 cut(s) 217, 413, 549, 705
BseDI CCNNGG 4 cut(s) 129, 216, 695, 703
BseMI GCAATG 1 cut(s) 606
BseXI GCAGC 2 cut(s) 361, 583
BseYI CCCAGC 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 460
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 89
BsiSI CCGG 2 cut(s) 460, 687
BslFI GGGAC 3 cut(s) 127, 575, 657
BsmAI GTCTC 1 cut(s) 410
BsmFI GGGAC 3 cut(s) 127, 575, 657
BsmI GAATGC 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 89
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 3 cut(s) 186, 299, 622
Bsp19I CCATGG 1 cut(s) 695
BspACI CCGC 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 645, 701
BspPI GGATC 2 cut(s) 194, 307
BspQI GCTCTTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 471
BssECI CCNNGG 4 cut(s) 129, 216, 695, 703
BssMI GATC 3 cut(s) 186, 299, 622
BssT1I CCWWGG 1 cut(s) 695
Bst2UI CCWGG 4 cut(s) 217, 413, 549, 705
Bst4CI ACNGT 2 cut(s) 421, 675
Bst6I CTCTTC 2 cut(s) 94, 103
BstAUI TGTACA 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 57, 74
BstDEI CTNAG 1 cut(s) 530
BstDSI CCRYGG 1 cut(s) 695
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 2 cut(s) 78, 633
BstKTI GATC 3 cut(s) 189, 302, 625
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 3 cut(s) 186, 299, 622
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 4 cut(s) 84, 324, 584, 593
BstNI CCWGG 4 cut(s) 217, 413, 549, 705
BstNSI RCATGY 2 cut(s) 275, 346
BstSCI CCNGG 6 cut(s) 215, 411, 459, 547, 685, 703
BstSFI CTRYAG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 361, 583
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BtgI CCRYGG 1 cut(s) 695
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 2 cut(s) 57, 74
CfoI GCGC 2 cut(s) 78, 633
Cfr13I GGNCC 2 cut(s) 239, 644
CseI GACGC 1 cut(s) 319
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 2 cut(s) 245, 472
CviAII CATG 9 cut(s) 272, 293, 307, 343, 378, 523, 558, 696, 710
CviQI GTAC 2 cut(s) 245, 472
DdeI CTNAG 1 cut(s) 530
DpnI GATC 3 cut(s) 188, 301, 624
DpnII GATC 3 cut(s) 186, 299, 622
Eam1104I CTCTTC 2 cut(s) 94, 103
EarI CTCTTC 2 cut(s) 94, 103
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 1 cut(s) 89
Eco47I GGWCC 2 cut(s) 239, 644
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 87
EcoICRI GAGCTC 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 644
EcoRII CCWGG 4 cut(s) 215, 411, 547, 703
EcoT14I CCWWGG 1 cut(s) 695
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 695
FaeI CATG 9 cut(s) 275, 296, 310, 346, 381, 526, 561, 699, 713
FaqI GGGAC 3 cut(s) 127, 575, 657
FatI CATG 9 cut(s) 271, 292, 306, 342, 377, 522, 557, 695, 709
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 3 cut(s) 375, 576, 597
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 3 cut(s) 375, 576, 597
FspBI CTAG 1 cut(s) 620
GlaI GCGC 2 cut(s) 77, 632
GluI GCNGC 3 cut(s) 375, 576, 597
GsaI CCCAGC 1 cut(s) 82
GsuI CTGGAG 1 cut(s) 434
HaeII RGCGCY 1 cut(s) 79
HapII CCGG 2 cut(s) 460, 687
HgaI GACGC 1 cut(s) 319
HhaI GCGC 2 cut(s) 78, 633
Hin1II CATG 9 cut(s) 275, 296, 310, 346, 381, 526, 561, 699, 713
Hin6I GCGC 2 cut(s) 76, 631
HinP1I GCGC 2 cut(s) 76, 631
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 3 cut(s) 17, 134, 658
HpaII CCGG 2 cut(s) 460, 687
HphI GGTGA 2 cut(s) 563, 619
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 4 cut(s) 45, 133, 299, 657
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 1 cut(s) 457
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 3 cut(s) 177, 268, 272
HpyCH4III ACNGT 2 cut(s) 421, 675
HpyCH4V TGCA 4 cut(s) 377, 557, 599, 678
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 324, 584, 593
HpyF3I CTNAG 1 cut(s) 530
Hsp92II CATG 9 cut(s) 275, 296, 310, 346, 381, 526, 561, 699, 713
HspAI GCGC 2 cut(s) 76, 631
Kzo9I GATC 3 cut(s) 186, 299, 622
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 361, 583
LweI GCATC 2 cut(s) 544, 574
MabI ACCWGGT 1 cut(s) 547
MaeI CTAG 1 cut(s) 620
MaeIII GTNAC 1 cut(s) 607
MalI GATC 3 cut(s) 188, 301, 624
MboI GATC 3 cut(s) 186, 299, 622
MboII GAAGA 9 cut(s) 12, 15, 18, 21, 24, 27, 30, 81, 90
MfeI CAATTG 1 cut(s) 359
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 2 cut(s) 359, 449
MmeI TCCRAC 3 cut(s) 160, 277, 456
MnlI CCTC 3 cut(s) 139, 153, 364
Mph1103I ATGCAT 1 cut(s) 559
MspI CCGG 2 cut(s) 460, 687
MspR9I CCNGG 6 cut(s) 217, 413, 461, 549, 687, 705
MunI CAATTG 1 cut(s) 359
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 4 cut(s) 217, 413, 549, 705
MwoI GCNNNNNNNGC 4 cut(s) 84, 324, 584, 593
NciI CCSGG 2 cut(s) 461, 687
NcoI CCATGG 1 cut(s) 695
NdeII GATC 3 cut(s) 186, 299, 622
NlaIII CATG 9 cut(s) 275, 296, 310, 346, 381, 526, 561, 699, 713
NlaIV GGNNCC 2 cut(s) 645, 701
NmuCI GTSAC 1 cut(s) 607
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 2 cut(s) 275, 346
PciI ACATGT 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 3 cut(s) 17, 134, 658
PkrI GCNGC 3 cut(s) 376, 577, 598
PpuMI RGGWCCY 1 cut(s) 644
PscI ACATGT 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 4 cut(s) 215, 411, 547, 703
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 4 cut(s) 215, 411, 547, 703
PspN4I GGNNCC 2 cut(s) 645, 701
PspPI GGNCC 2 cut(s) 239, 644
PspPPI RGGWCCY 1 cut(s) 644
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 2 cut(s) 246, 473
RsaNI GTAC 2 cut(s) 245, 472
SacI GAGCTC 1 cut(s) 89
SalI GTCGAC 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 94
SatI GCNGC 3 cut(s) 375, 576, 597
Sau3AI GATC 3 cut(s) 186, 299, 622
Sau96I GGNCC 2 cut(s) 239, 644
ScrFI CCNGG 6 cut(s) 217, 413, 461, 549, 687, 705
SduI GDGCHC 1 cut(s) 89
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 2 cut(s) 544, 574
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 2 cut(s) 239, 644
Sse9I AATT 2 cut(s) 359, 449
SsiI CCGC 1 cut(s) 576
SspMI CTAG 1 cut(s) 620
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 6 cut(s) 215, 411, 459, 547, 685, 703
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 2 cut(s) 421, 675
TaqI TCGA 1 cut(s) 456
TasI AATT 2 cut(s) 359, 449
TatI WGTACW 1 cut(s) 471
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 3 cut(s) 17, 134, 658
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 2 cut(s) 374, 596
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 3 cut(s) 391, 462, 496
TspRI CASTG 1 cut(s) 680
VpaK11BI GGWCC 2 cut(s) 239, 644
XapI RAATTY 1 cut(s) 449
XceI RCATGY 2 cut(s) 275, 346
XcmI CCANNNNNNNNNTGG 2 cut(s) 75, 586
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 1 cut(s) 620
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.