AT5G57040

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008043
Physical Location & Seq
Reverse (-)
23083898 .. 23085246
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57040.1

Sequence Viewer

Length: 594 bp
ATGGCTTCTATTTTCAGACCTTCTTCAGCTTCACTTGATTTACGACCAAAGGTTATTTGCACGAATCTCTCTACGAAAGAGCGGTTTGAGTTTCAGAAGAAGAGTGTGAGGAAAGAAAGGATCAATGTTAGGTTTTATAGCTTGAAGGCTAAGGCACAAGGATCGTCAATTGAAGGGATTAGTGTTGTACAAGAGAAAGAGCTTAACAACAAAACCGATTATGGAGTTGTTGGGGTTCATCATGTCGGTCTGCTCTGCGAGAACCTAGAACGGTCACTCGAGTTTTACCAGAACATTTTAGGCCTTGAGATCAACGAAGCAAGGCCTCACGACAAGCTTCCATATAGAGGAGCATGGTTATGGGTAGGTTCAGAAATGATTCATCTTATGGAGCTTCCAAATCCAGATCCATTAACAGGTAGACCCGAGCACGGAGGCCGGGATCGACACGCTTGTATTGCAATCCGTGATGTTTCAAATCTGAAAGAGATCTTAGACAAAGCTGGAATTGCATATACAATGAGTAAGTCAGGGAGGCCAGCGATATTTACTCGTGATCCAGACGCAAACGCTCTTGAGTTCACCCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

197

Amino Acids

22.25

Weight (kDa)

8.75

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 79 - 194 8.4e-11 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 421
AciI CCGC 1 cut(s) 82
AclWI GGATC 5 cut(s) 128, 169, 401, 450, 551
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 3 cut(s) 347, 416, 431
AgsI TTSAA 3 cut(s) 145, 173, 477
AluBI AGCT 6 cut(s) 29, 141, 202, 337, 394, 503
AluI AGCT 6 cut(s) 29, 141, 202, 337, 394, 503
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 5 cut(s) 128, 169, 401, 450, 551
Ama87I CYCGRG 2 cut(s) 278, 425
AoxI GGCC 4 cut(s) 301, 323, 436, 536
Asp700I GAANNNNTTC 1 cut(s) 378
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 574
AvaI CYCGRG 2 cut(s) 278, 425
BauI CACGAG 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 432
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BcnI CCSGG 1 cut(s) 440
BfaI CTAG 1 cut(s) 266
BglII AGATCT 1 cut(s) 489
Bme1390I CCNGG 1 cut(s) 440
BmeT110I CYCGRG 2 cut(s) 278, 425
BmrFI CCNGG 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 440
Bsc4I CCNNNNNNNGG 3 cut(s) 347, 416, 431
BseLI CCNNNNNNNGG 3 cut(s) 347, 416, 431
BseRI GAGGAG 1 cut(s) 363
BshFI GGCC 4 cut(s) 303, 325, 438, 538
BsiHKAI GWGCWC 1 cut(s) 432
BsiHKCI CYCGRG 2 cut(s) 278, 425
BsiSI CCGG 1 cut(s) 439
BslI CCNNNNNNNGG 3 cut(s) 347, 416, 431
BsnI GGCC 4 cut(s) 303, 325, 438, 538
BsoBI CYCGRG 2 cut(s) 278, 425
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
BspACI CCGC 1 cut(s) 82
BspANI GGCC 4 cut(s) 303, 325, 438, 538
BspPI GGATC 5 cut(s) 128, 169, 401, 450, 551
BsrBI CCGCTC 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 187
BssMI GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
BssSI CACGAG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 552
Bst4CI ACNGT 1 cut(s) 273
Bst6I CTCTTC 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 187
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 150, 493
BstKTI GATC 7 cut(s) 123, 164, 312, 409, 445, 492, 559
BstMBI GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
BstMWI GCNNNNNNNGC 2 cut(s) 458, 509
BstSCI CCNGG 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 406, 489
BstYI RGATCY 2 cut(s) 406, 489
BsuRI GGCC 4 cut(s) 303, 325, 438, 538
Cac8I GCNNGC 1 cut(s) 540
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 188
CviAII CATG 2 cut(s) 242, 354
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 2 cut(s) 150, 493
DpnI GATC 7 cut(s) 122, 163, 311, 408, 444, 491, 558
DpnII GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco147I AGGCCT 2 cut(s) 303, 325
Eco57I CTGAAG 1 cut(s) 9
Eco88I CYCGRG 2 cut(s) 278, 425
FaeI CATG 2 cut(s) 245, 357
FatI CATG 2 cut(s) 241, 353
FblI GTMKAC 1 cut(s) 421
FspBI CTAG 1 cut(s) 266
HaeIII GGCC 4 cut(s) 303, 325, 438, 538
HapII CCGG 1 cut(s) 439
HgaI GACGC 1 cut(s) 572
Hin1II CATG 2 cut(s) 245, 357
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 2 cut(s) 64, 379
HpaII CCGG 1 cut(s) 439
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 2 cut(s) 422, 582
Hpy188I TCNGA 4 cut(s) 17, 96, 373, 483
Hpy188III TCNNGA 5 cut(s) 329, 404, 554, 560, 575
Hpy8I GTNNAC 2 cut(s) 422, 582
HpyAV CCTTC 3 cut(s) 30, 139, 167
HpyCH4III ACNGT 1 cut(s) 273
HpyCH4V TGCA 3 cut(s) 60, 461, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 509
HpyF3I CTNAG 2 cut(s) 150, 493
Hsp92II CATG 2 cut(s) 245, 357
Kzo9I GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
LmnI GCTCC 2 cut(s) 350, 391
LpnPI CCDG 8 cut(s) 302, 402, 417, 452, 489, 516, 552, 573
MaeI CTAG 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 273
MalI GATC 7 cut(s) 122, 163, 311, 408, 444, 491, 558
MbiI CCGCTC 1 cut(s) 82
MboI GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
MboII GAAGA 3 cut(s) 15, 109, 112
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 2 cut(s) 406, 489
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 2 cut(s) 168, 507
MnlI CCTC 5 cut(s) 102, 336, 341, 428, 528
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 2 cut(s) 204, 413
MslI CAYNNNNRTG 1 cut(s) 358
MspI CCGG 1 cut(s) 439
MspR9I CCNGG 1 cut(s) 440
MunI CAATTG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 458, 509
NciI CCSGG 1 cut(s) 440
NdeII GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
NlaIII CATG 2 cut(s) 245, 357
NmuCI GTSAC 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 278
PceI AGGCCT 2 cut(s) 303, 325
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 2 cut(s) 64, 379
PspXI VCTCGAGB 1 cut(s) 278
PsuI RGATCY 2 cut(s) 406, 489
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 358
SaqAI TTAA 2 cut(s) 204, 413
Sau3AI GATC 7 cut(s) 120, 161, 309, 406, 442, 489, 556
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 358
SmlI CTYRAG 3 cut(s) 278, 305, 575
SmoI CTYRAG 3 cut(s) 278, 305, 575
Sse9I AATT 2 cut(s) 168, 507
SseBI AGGCCT 2 cut(s) 303, 325
SsiI CCGC 1 cut(s) 82
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 2 cut(s) 303, 325
StyD4I CCNGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 279, 445
TaqII GACCGA 1 cut(s) 236
TasI AATT 2 cut(s) 168, 507
TatI WGTACW 1 cut(s) 187
TfiI GAWTC 2 cut(s) 64, 379
Tru1I TTAA 2 cut(s) 204, 413
Tru9I TTAA 2 cut(s) 204, 413
TseFI GTSAC 1 cut(s) 273
Tsp45I GTSAC 1 cut(s) 273
TspDTI ATGAA 2 cut(s) 227, 371
TspGWI ACGGA 2 cut(s) 447, 455
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.