MD15G1211500.v1.1
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008043
Physical Location & Seq
Reverse (-)
16989068 .. 16992050
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1211500.v1.1.491

Sequence Viewer

Length: 612 bp
ATGAGCAGCTGCTCTTCCATGGCTTCCCTTCTCATCCCACCTCCCACCCTTCCCCTCCAACTCAAGGAAGATGGGATTAGAGTCAGCACTTTACTTACGAACCCATCATCTAAAATTGACCGGACCGGTCTGAGAAGTTGCAGGCGTCACGGACGGTGTTCTGTGGCGAAAGCTAAGGCGACCGTTGAAGAAGATGTACTGGAGAAGCAATCAGTCGGTGCTCAGGACAAAAATGATTTTGGAGTTGTTAGTGTCCACCATGTCGGATTGCTGTGTGAAAACCTTGAAAGATCTCTCAGCTTTTACCAGAACATTTTGGGGCTTGAAATAAATGAGGCAAGGCCACATGATAAGCTCCCCTACAGAGGTGCTTGGTTATGGGTTGGTTCAGAGATGATTCATCTCATGGAACTTCCAAATCCCGACCCTTTGACCGGAAGACCAACGCATGGAGGCCGCGACCGCCATACTTGTATTGCAATCCGGGATGTGTCTAAGCTGAAAGTAATCCTCGACGATGCTGGTATCCCCTACACGCTGAGCAAGTCCGGGAGGCCGGCGATTTTCACTCGAGACCCGGATGCAAATGCTCTGGAGTTTACTCAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

204

Amino Acids

22.39

Weight (kDa)

7.7

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 85 - 200 1.1e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 449
AccII CGCG 1 cut(s) 459
AciI CCGC 2 cut(s) 457, 463
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 4 cut(s) 64, 365, 434, 449
AgeI ACCGGT 1 cut(s) 125
AgsI TTSAA 3 cut(s) 188, 287, 326
AluBI AGCT 5 cut(s) 9, 173, 300, 355, 499
AluI AGCT 5 cut(s) 9, 173, 300, 355, 499
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 567
Ama87I CYCGRG 1 cut(s) 570
AoxI GGCC 3 cut(s) 341, 454, 554
ApeKI GCWGC 2 cut(s) 6, 9
AsiGI ACCGGT 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 123
AsuC2I CCSGG 3 cut(s) 485, 550, 578
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 1 cut(s) 123
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 18
BccI CCATC 2 cut(s) 65, 112
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 3 cut(s) 485, 550, 578
BcoDI GTCTC 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 361
BfuI GTATCC 1 cut(s) 536
BglII AGATCT 1 cut(s) 290
BisI GCNGC 3 cut(s) 7, 10, 457
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 3 cut(s) 8, 11, 458
Bme1390I CCNGG 3 cut(s) 485, 550, 578
Bme18I GGWCC 1 cut(s) 123
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 1 cut(s) 123
BmrFI CCNGG 3 cut(s) 485, 550, 578
BmsI GCATC 2 cut(s) 508, 571
BpiI GAAGAC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 174, 222
Bpu1102I GCTNAGC 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 47, 588
BpuMI CCSGG 3 cut(s) 485, 550, 578
BsaHI GRCGYC 1 cut(s) 145
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 3 cut(s) 120, 125, 434
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 365, 434, 449
Bse118I RCCGGY 2 cut(s) 125, 556
Bse1I ACTGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 3 cut(s) 33, 493, 586
BseLI CCNNNNNNNGG 4 cut(s) 64, 365, 434, 449
BseMII CTCAG 4 cut(s) 122, 236, 310, 530
BseNI ACTGG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 459
Bsh1285I CGRYCG 2 cut(s) 183, 463
BshFI GGCC 3 cut(s) 343, 456, 556
BshTI ACCGGT 1 cut(s) 125
BsiEI CGRYCG 2 cut(s) 183, 463
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 7 cut(s) 121, 126, 435, 484, 549, 557, 578
BslI CCNNNNNNNGG 4 cut(s) 64, 365, 434, 449
BsmAI GTCTC 1 cut(s) 567
BsnI GGCC 3 cut(s) 343, 456, 556
Bso31I GGTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 290
Bsp1720I GCTNAGC 1 cut(s) 539
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 2 cut(s) 457, 463
BspANI GGCC 3 cut(s) 343, 456, 556
BspCNI CTCAG 4 cut(s) 123, 235, 309, 531
BspFNI CGCG 1 cut(s) 459
BspQI GCTCTTC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 567
BsrFI RCCGGY 2 cut(s) 125, 556
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 2 cut(s) 125, 556
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 1 cut(s) 290
BssNI GRCGYC 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 156, 184
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 143, 558
BstDEI CTNAG 6 cut(s) 131, 174, 222, 296, 495, 539
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 3 cut(s) 33, 493, 586
BstFNI CGCG 1 cut(s) 459
BstKTI GATC 1 cut(s) 293
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 1 cut(s) 290
BstMCI CGRYCG 2 cut(s) 183, 463
BstMWI GCNNNNNNNGC 1 cut(s) 462
BstSCI CCNGG 3 cut(s) 483, 548, 576
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 3 cut(s) 343, 456, 556
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 3 cut(s) 33, 493, 586
Cac8I GCNNGC 2 cut(s) 143, 558
Cfr10I RCCGGY 2 cut(s) 125, 556
Cfr13I GGNCC 1 cut(s) 123
CpoI CGGWCCG 1 cut(s) 123
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 197
CspAI ACCGGT 1 cut(s) 125
CspI CGGWCCG 1 cut(s) 123
CviAII CATG 5 cut(s) 19, 260, 347, 406, 449
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 6 cut(s) 131, 174, 222, 296, 495, 539
DpnI GATC 1 cut(s) 292
DpnII GATC 1 cut(s) 290
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 5 cut(s) 22, 263, 350, 409, 452
FaiI YATR 7 cut(s) 20, 261, 348, 379, 407, 450, 468
FatI CATG 5 cut(s) 18, 259, 346, 405, 448
Fnu4HI GCNGC 3 cut(s) 7, 10, 457
FokI GGATG 3 cut(s) 20, 500, 593
Fsp4HI GCNGC 3 cut(s) 7, 10, 457
GluI GCNGC 3 cut(s) 7, 10, 457
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 3 cut(s) 343, 456, 556
HapII CCGG 7 cut(s) 121, 126, 435, 484, 549, 557, 578
HgaI GACGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 5 cut(s) 22, 263, 350, 409, 452
HinfI GANTC 2 cut(s) 81, 397
HpaII CCGG 7 cut(s) 121, 126, 435, 484, 549, 557, 578
Hpy166II GTNNAC 2 cut(s) 256, 600
Hpy188I TCNGA 3 cut(s) 132, 266, 391
Hpy188III TCNNGA 4 cut(s) 224, 422, 572, 593
Hpy8I GTNNAC 2 cut(s) 256, 600
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 38, 59
HpyCH4III ACNGT 2 cut(s) 156, 184
HpyCH4V TGCA 3 cut(s) 141, 479, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 462
HpyF3I CTNAG 6 cut(s) 131, 174, 222, 296, 495, 539
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 5 cut(s) 22, 263, 350, 409, 452
KroI GCCGGC 1 cut(s) 556
KroNI GCCGGC 1 cut(s) 558
Kzo9I GATC 1 cut(s) 290
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 2 cut(s) 508, 571
MaeIII GTNAC 1 cut(s) 146
MalI GATC 1 cut(s) 292
MboI GATC 1 cut(s) 290
MboII GAAGA 5 cut(s) 6, 80, 200, 203, 450
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 114
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 82, 244
MnlI CCTC 7 cut(s) 51, 65, 328, 359, 446, 521, 546
MroNI GCCGGC 1 cut(s) 556
MseI TTAA 1 cut(s) 610
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 7 cut(s) 121, 126, 435, 484, 549, 557, 578
MspR9I CCNGG 3 cut(s) 485, 550, 578
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 1 cut(s) 462
NaeI GCCGGC 1 cut(s) 558
NciI CCSGG 3 cut(s) 485, 550, 578
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 290
NgoMIV GCCGGC 1 cut(s) 556
NlaIII CATG 5 cut(s) 22, 263, 350, 409, 452
NmuCI GTSAC 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 570
PciSI GCTCTTC 1 cut(s) 19
PdiI GCCGGC 1 cut(s) 558
PfeI GAWTC 1 cut(s) 397
PflMI CCANNNNNTGG 1 cut(s) 449
PfoI TCCNGGA 2 cut(s) 483, 548
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 3 cut(s) 8, 11, 458
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 123
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
Rsr2I CGGWCCG 1 cut(s) 123
RsrII CGGWCCG 1 cut(s) 123
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 1 cut(s) 610
SatI GCNGC 3 cut(s) 7, 10, 457
Sau3AI GATC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 123
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 3 cut(s) 485, 550, 578
SduI GDGCHC 1 cut(s) 223
SetI ASST 8 cut(s) 11, 43, 175, 285, 302, 357, 370, 501
SfaNI GCATC 2 cut(s) 508, 571
SfcI CTRYAG 1 cut(s) 361
Sfr274I CTCGAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 123
SlaI CTCGAG 1 cut(s) 570
SmlI CTYRAG 3 cut(s) 62, 570, 603
SmoI CTYRAG 3 cut(s) 62, 570, 603
Sse9I AATT 1 cut(s) 114
SsiI CCGC 2 cut(s) 457, 463
StyD4I CCNGG 3 cut(s) 483, 548, 576
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 156, 184
TaqI TCGA 2 cut(s) 513, 571
TasI AATT 1 cut(s) 114
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 459
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 610
Tru9I TTAA 1 cut(s) 610
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 2 cut(s) 6, 9
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 165
Van91I CCANNNNNTGG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.