RLG00000001330
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008043
Physical Location & Seq
Reverse (-)
14153550 .. 14154738
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001330

Sequence Viewer

Length: 564 bp
ATGAGCAGCAGCTCTTCCATGGCTTCCCTTCTCACCCCAACCCCAACCATTACACTCCAACACAAGAAAGATCATTTCAGGCTCAACACAAACCCACCACCCAGAATTTCCAAGAGAAATTTTGGGCGCCATGGGCTTTGTTTTGTGACAAGAGCTAAGGTTGGGGGAGATGTACTTGAGAAACAATCAGTTAGTGCTGAAGACAAGAAGGCTCATTTGGTGATACTGATCCAGATTATGGCCTGGTTAGTGTCCACCATGTTAATCTTGTGTGAAAACTTGGAGAGGTCGCTCGACTTTTATCAGAACATTCTTGGTCTTGAAATTAATGAGGCTAGGCCAGATGACAAACTCCCCTATAGGGGGGCTTGGTTATGGGTGGGTTCTGAGATGATTCACCTTATGGAGCTTCCAAACCCCGACCCTGTAACCGGAAGACCGGAGCATGGAGGCCGTGATAGGCACACGGTATTCCATACACGCTTAGTAAGTCTGGGAGGCCAGCAATCTTTTTTCGAGGTCCAGATGAAAATGCTCTGGAATTTGCACAGTTCTGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

188

Amino Acids

21.2

Weight (kDa)

8.63

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 88 - 159 6e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 238
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 3 cut(s) 105, 118, 541
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 6 cut(s) 238, 361, 362, 363, 431, 446
AgsI TTSAA 1 cut(s) 323
AjnI CCWGG 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 3 cut(s) 12, 155, 409
AluI AGCT 3 cut(s) 12, 155, 409
AlwI GGATC 1 cut(s) 223
AoxI GGCC 4 cut(s) 240, 338, 451, 499
ApeKI GCWGC 2 cut(s) 6, 9
ApoI RAATTY 3 cut(s) 105, 118, 541
AseI ATTAAT 1 cut(s) 327
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 520
AsuHPI GGTGA 3 cut(s) 25, 232, 389
AvaII GGWCC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 207, 442
BbvI GCAGC 2 cut(s) 18, 21
BccI CCATC 1 cut(s) 551
BceAI ACGGC 1 cut(s) 438
BciT130I CCWGG 1 cut(s) 244
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 358
BfoI RGCGCY 1 cut(s) 130
BisI GCNGC 2 cut(s) 7, 10
BlsI GCNGC 2 cut(s) 8, 11
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 1 cut(s) 520
BmgT120I GGNCC 1 cut(s) 520
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 207, 442
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 18, 130
BsaWI WCCGGW 2 cut(s) 431, 439
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 361, 362, 363, 431, 446
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 18, 130
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 6 cut(s) 238, 361, 362, 363, 431, 446
BseMII CTCAG 1 cut(s) 378
BseXI GCAGC 2 cut(s) 18, 21
BshFI GGCC 4 cut(s) 242, 340, 453, 501
BshNI GGYRCC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 432, 440
BslI CCNNNNNNNGG 6 cut(s) 238, 361, 362, 363, 431, 446
BsnI GGCC 4 cut(s) 242, 340, 453, 501
Bsp143I GATC 2 cut(s) 70, 228
Bsp19I CCATGG 2 cut(s) 18, 130
BspANI GGCC 4 cut(s) 242, 340, 453, 501
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 126
BssECI CCNNGG 2 cut(s) 18, 130
BssMI GATC 2 cut(s) 70, 228
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 2 cut(s) 18, 130
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 469, 551
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 503
BstDEI CTNAG 3 cut(s) 156, 387, 484
BstDSI CCRYGG 2 cut(s) 18, 130
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 2 cut(s) 73, 231
BstMBI GATC 2 cut(s) 70, 228
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 2 cut(s) 18, 21
BstV2I GAAGAC 2 cut(s) 207, 442
BsuRI GGCC 4 cut(s) 242, 340, 453, 501
BtgI CCRYGG 2 cut(s) 18, 130
Cac8I GCNNGC 1 cut(s) 503
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 520
Csp6I GTAC 1 cut(s) 173
CviAII CATG 4 cut(s) 19, 131, 259, 446
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 3 cut(s) 156, 387, 484
DinI GGCGCC 1 cut(s) 128
DpnI GATC 2 cut(s) 72, 230
DpnII GATC 2 cut(s) 70, 228
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 18, 130
Eco47I GGWCC 1 cut(s) 520
Eco57I CTGAAG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 18, 130
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 18, 130
FaeI CATG 4 cut(s) 22, 134, 262, 449
FaiI YATR 9 cut(s) 20, 132, 239, 260, 360, 376, 404, 447, 477
FatI CATG 4 cut(s) 18, 130, 258, 445
Fnu4HI GCNGC 2 cut(s) 7, 10
Fsp4HI GCNGC 2 cut(s) 7, 10
FspBI CTAG 1 cut(s) 336
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 7, 10
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 4 cut(s) 242, 340, 453, 501
HapII CCGG 2 cut(s) 432, 440
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 4 cut(s) 22, 134, 262, 449
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 1 cut(s) 394
HpaII CCGG 2 cut(s) 432, 440
HphI GGTGA 3 cut(s) 25, 232, 389
Hpy166II GTNNAC 1 cut(s) 255
Hpy188I TCNGA 3 cut(s) 306, 388, 556
Hpy188III TCNNGA 4 cut(s) 232, 320, 523, 538
Hpy8I GTNNAC 1 cut(s) 255
HpyAV CCTTC 2 cut(s) 38, 202
HpyCH4III ACNGT 2 cut(s) 469, 551
HpyCH4V TGCA 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 3 cut(s) 156, 387, 484
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 4 cut(s) 22, 134, 262, 449
HspAI GCGC 1 cut(s) 127
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 70, 228
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 406, 442
Lsp1109I GCAGC 2 cut(s) 18, 21
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 145, 427
MalI GATC 2 cut(s) 72, 230
MboI GATC 2 cut(s) 70, 228
MboII GAAGA 3 cut(s) 6, 212, 447
MluCI AATT 4 cut(s) 105, 118, 324, 541
Mly113I GGCGCC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 5 cut(s) 279, 325, 443, 491, 511
MseI TTAA 2 cut(s) 263, 327
MspI CCGG 2 cut(s) 432, 440
MspR9I CCNGG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 1 cut(s) 133
NarI GGCGCC 1 cut(s) 127
NcoI CCATGG 2 cut(s) 18, 130
NdeII GATC 2 cut(s) 70, 228
NlaIII CATG 4 cut(s) 22, 134, 262, 449
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 19
PfeI GAWTC 1 cut(s) 394
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 2 cut(s) 8, 11
PluTI GGCGCC 1 cut(s) 130
PshBI ATTAAT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 520
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 2 cut(s) 263, 327
SatI GCNGC 2 cut(s) 7, 10
Sau3AI GATC 2 cut(s) 70, 228
Sau96I GGNCC 1 cut(s) 520
ScrFI CCNGG 1 cut(s) 244
SetI ASST 7 cut(s) 14, 157, 162, 290, 402, 411, 522
SfcI CTRYAG 1 cut(s) 358
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 4 cut(s) 105, 118, 324, 541
SspDI GGCGCC 1 cut(s) 126
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 2 cut(s) 18, 130
TaaI ACNGT 2 cut(s) 469, 551
TaqI TCGA 2 cut(s) 294, 516
TasI AATT 4 cut(s) 105, 118, 324, 541
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 2 cut(s) 263, 327
Tru9I TTAA 2 cut(s) 263, 327
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 6, 9
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 542
Van91I CCANNNNNTGG 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 520
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 3 cut(s) 105, 118, 541
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.